JPH05306978A - Microorganism measuring instrument - Google Patents

Abstract

PURPOSE:To obtain a microorganism measuring instrument which can automatically and easily collect microorganisms in a short time and, at the same time, can be increased and stabilized in microorganism recovery. CONSTITUTION:This instrument is provided with a sample water flowing-in means, microorganism disintegrating liquid injecting means, filter cartridge 15 having a microorganism catching filter 45 which divides an outer tube 41 into two parts on a sample water flowing-in port 42 and sample water flowing- out port 43 sides in the outer tube provided with the ports 42 and 43, filter cartridge feeding device, transferring device, microorganism disintegrating device 16, and sample water collecting device and the measurement of microorganism is automated by means of a control means which controls the measuring apparatus of the instrument.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for immunologically measuring microorganisms, and more particularly to pretreatment for measuring the number of microorganisms in sample water.

[0002]

2. Description of the Related Art Conventionally, the measurement of microorganisms in sample water has been carried out by concentrating the sample water with a membrane filter and ultrasonically crushing it, and then performing immunoassay.

When the sample water is concentrated with this membrane filter, it is necessary to use a pre-filter in order to shorten the concentration time and remove impurities.

FIG. 3 shows an explanatory view of a microorganism concentrating device using the above prefilter and membrane filter.

As shown in this figure, this microorganism concentrating apparatus has a funnel 51 for containing sample water, a filtering section 52 for filtering and concentrating the sample water, a side flask 53 for containing the filtered and concentrated sample water, and a funnel. It is composed of fixed scissors 56 for fixing 51 and the side flask 53.

FIG. 4 is an enlarged view of the filtration section of this microorganism concentrating device. As shown in this figure, the filter section 62 is formed by arranging a funnel 51, an O-ring 64, a membrane filter 65, and a side flask 53 in this order.

In order to concentrate the microorganisms with the above apparatus, sample water is put into the funnel 51, and the internal pressure is lowered by a suction pump through the side pipe of the side flask 53. As a result, the sample water is sucked into the side flask.

At this time, since the microorganisms in the sample water are captured by the membrane filter 65 of the filtration section, the membrane filter 65 is collected, and the buffer solution and the glass beads are added and shaken in the glass beaker.

Thus, the bacteria captured on the surface of the membrane filter 65 are uniformly suspended in the buffer solution, and then the suspension is pipetted and transferred to a sample tube.

Furthermore, the microorganisms in the sample water are measured by ultrasonically disrupting the suspension in the sample tube with an ultrasonic disruptor and then performing enzyme immunoassay.

[0011]

However, in the above-mentioned conventional measuring method, the collecting operation, which is a pretreatment of the enzyme immunoassay, is complicated and the collecting rate is difficult to be constant, and the measuring accuracy becomes low. Further, the recovery operation requires about 15 minutes, which causes a large loss of time.

Further, a large-scale apparatus or instrument such as a filtering apparatus such as a side-branched flask or a funnel and a shaker used at the time of recovery is required, and automation of pretreatment is also difficult.

The present invention has been made under the above-mentioned background, and a microbial immunology capable of performing a microbial recovery operation easily and in a short time and having a high microbial recovery rate and stabilization. An object of the present invention is to provide a method for dynamic measurement and a microorganism filtration container.

[0014]

In order to solve the above problems, the present invention provides an inside of an outer cylinder having a sample water inlet and a filtered water outlet, and the inside of the outer cylinder is a sample water inlet side and a sample water outlet side. A filter cartridge having a microbial capture filter for partitioning into sample, sample water supply means for supplying sample water, sample water inflow means for injecting sample water obtained from the sample water supply means into the filter cartridge, and inflow into the filter cartridge Forced filtering means for forcibly filtering the sample water to be separated into a microorganism and a filtrate that are captured as a residue on the microorganism trapping filter, a microorganism crushed liquid supply means for supplying a microbial crushed liquid, and the sample water After the completion of the filtration of (1), the microbial disruption liquid obtained from the microbial disruption liquid supply means is mixed with the microbial capture filter in the filter cartridge and the outside. Microorganism crushed liquid injection means for injecting into the gap with the sample water outflow side, and having the filter cartridge in a position capable of crushing microorganisms in advance, the microbial crushed liquid after the injection of the microbial crushed liquid into the gap is completed. Microorganism measurement having microbial crushing means for crushing, microbial measuring means, and microbial crushed liquid transporting means for transporting microbial crushed liquid injected into the gap to the microbial measuring means after crushing of the microorganisms is completed Provide the device.

Further, in the above-mentioned microorganism measuring apparatus, the microorganism measuring apparatus comprises means for removing crushed liquid of microorganisms remaining in the filter cartridge after the crushed liquid of microorganisms is transported to the means for measuring microorganisms. By introducing the cleaning water supply means and the cleaning water obtained from the cleaning water supply means into the filter cartridge,
After cleaning the microorganism-trapping filter, and thereby ending the washing of the microorganism-trapping filter, a microorganism-trapping filter cleaning means capable of repeatedly measuring the sample water obtained from the sample water supply means is provided. Also provided is a microorganism measuring device characterized by:

[0016]

According to the present invention, there is provided a filter cartridge having a microorganism trapping filter inside the outer cylinder having the sample water inlet and the filtered water outlet, which partition the inside of the outer cylinder into the sample water inlet side and the sample water outlet side. To use.

The sample water obtained from the sample water supply means flows into this filter cartridge into the filter cartridge, and the sample water is forcibly filtered to separate into microorganisms and filtrate that are captured as a residue on the microorganism capture filter. ..

Usually, in order to filter the sample water containing microorganisms, a filter having a small pore size such as a micro filter is used, and the microorganisms in the sample water are captured on the filter as the filtration progresses.

Therefore, in the filtration of the sample water on the filter by gravity as is generally done, the filtration hardly progresses, and as the filtration proceeds, the filter is clogged with the microorganisms trapped on the filter. When the filtration proceeds to some extent, the sample water hardly passes through the filter.

Therefore, when capturing the microorganisms, it is necessary to forcibly advance the filtration by suction filtration or pressure filtration.
In the present specification, such forced filtration, such as suction filtration, compression filtration, centrifugal filtration, etc., is referred to as forced filtration.

When the sample water is filtered by performing the forced filtration as described above, the filter becomes clogged, and the liquid such as the sample water and the microbial disruption liquid is considered not to pass through the filter.

As a result, when forced filtration is carried out using the above filter cartridge, the microorganism trapping filter becomes clogged, and liquid is trapped in the gap between the microorganism trapping filter in the filter cartridge and the sample water outflow side of the outer cylinder. It becomes possible to hold.

After the sample water is forcibly filtered by the filter cartridge in this way, the microorganism trapping filter becomes clogged. Therefore, if the microorganism disruption liquid is injected into the gap in this state, the microorganism disruption liquid will pass through the filter. It is stored in the gap without permeation.

On the other hand, since microorganisms are trapped in the filter, storing the crushed microorganism liquid in the gap results in the same state as when the filter was put into the crushed microorganism liquid.

As described above, the sample water is filtered using the filter cartridge having the gap, and after the filtration is finished, the microbial disruption liquid is stored in the gap, so that the same container (filter cartridge) is stored. Thus, the sample water can be filtered and the microorganism trapping filter can be added to the microorganism disruption liquid.

Therefore, conventionally, after the filtration of the sample solution is completed, it is necessary to prepare a container containing the microorganism crushing solution and a microorganism crushing solution in advance, and to insert the microbial catching filter into the microorganism crushing solution. However, such a complicated and labor-intensive operation becomes unnecessary.

The microorganisms are crushed by driving the microbial crushing means which has the filter cartridge in advance at a position where the microorganisms can be crushed while the microorganisms captured as described above and the microbial crushing liquid are in contact with each other. be able to.

After the crushing of the microorganisms is completed, the crushed liquid of the microorganisms injected into the gap is transported to the microorganism measuring means, whereby the microorganisms can be easily measured.

The sample water supply means used in the present invention is not particularly limited, and may be, for example, a sample water tank in which sample water is stored in advance, or a sample collecting device for automatically collecting wastewater from a sewage treatment plant. Can be mentioned. Similarly, examples of the microbial crushed liquid supply means include a microbial crushed liquid tank in which the microbial crushed liquid is stored in advance.

As the sample liquid inflow means, the microorganism crushed liquid injection means, and the microbial crushed liquid transport means, it is possible to easily flow in the sample liquid etc. by using a conventional method, for example, combining a tube and a pump. The measuring means is not particularly limited, and for example, a commonly used measuring instrument such as EIA can be used. Further, as the microorganism crushing means, a commonly used means can be used, and for example, an ultrasonic crusher or the like is used.

Preferably, by combining a valve (for example, an electromagnetic valve) with the above pipe or pump, it is possible to easily inflow the sample solution, inject and transport the microbial disruption solution, and the like.

Further, by providing the above-mentioned microorganism measuring apparatus with a measuring apparatus for measuring the inflow amount of sample water, crushed microorganisms, etc., and calculating the concentration ratio of microorganisms from these measurement results,
It is also possible to measure the microorganisms more accurately.

Further, after the microbial disruption liquid is transported to the microorganism measuring means, the residual microbial disruption liquid in the filter cartridge is removed, and then washing water is allowed to flow into the filter cartridge to wash the microorganism trapping filter. preferable.

This makes it possible to repeatedly measure the sample water obtained from the sample water supply means after the washing of the microorganism trapping filter is completed. For example, a treated water collecting device for collecting the treated water at a sewage treatment plant is used as the sample water. By using it as a sampling means, it is possible to easily continuously monitor changes in water quality in the sewage treatment plant.

When washing the microorganism trapping filter with the above-mentioned washing water, the washing water is preferably introduced from the filtered water outlet side of the filter cartridge. This further enhances the filter cleaning effect.

As described above, in the microorganism measuring apparatus according to the present invention, the sample water supplying means, the sample water inflowing means, the microbial crushed liquid supplying means, the microbial crushed liquid injecting means, the microbial crushing means, the microbial transporting means, the forced filtration. The microorganisms can be measured automatically by controlling the means and the like by the control device.

Automation by the above control device is very easy. For example, continuous measurement of sample water, which conventionally required manpower, such as change with time of treated water in a sewage treatment plant, can be automatically and easily performed. You can

Furthermore, a pressure gauge, a flow meter, etc. are provided in the above-mentioned microorganism measuring apparatus, and based on the information obtained from these, and the information obtained from the above-mentioned apparatus for measuring the inflow amount of the sample water, the microbial disruption liquid, etc. By controlling various control objects such as the valve and the sample liquid inflow means, it is possible to measure the microorganism more accurately and efficiently.

[0039]

Example In this example, ultrasonic disruption of microorganisms was performed using the filtration container (filter cartridge) shown in FIG.

In FIG. 2, reference numeral 41 is an outer cylinder having a sample water inlet 42 and a filtered water outlet 43.

Inside the outer cylinder 41, one end side is cylindrical and is opened. The opening side is arranged on the filtered water outlet 43 side so that the inside of the outer cylinder is on the sample water inlet 42 side and the filtered water outlet 43 side. A partitioning filter support portion 44 is provided. The opening side of the filter support may be arranged on the sample water outlet 42 side.

Further, the surface of the filter support portion 44 on the side of the sample water inlet 42 is covered with a microfilter 45 which is a microorganism trapping filter.

The filter support portion 44 is filtered by the micro filter 45 by a method such as being formed of a water permeable material or having a water permeable structure such as a metal mesh. The structure will allow water to pass through. Further, as the micro filter, one having a pore size of 0.45 μm or less is preferably used. Therefore, in the filtration container, the sample water flowing in from the sample water inlet 42 is always filtered by the microfilter 45 and then flows out from the filtered water outlet 43.

In particular, the microfilter 45 is usually filtered by suction filtration, compression filtration or the like, and it does not matter that the sample water or the crushed microorganism solution does not pass through the microfilter 45 under normal pressure.

Therefore, in the above-mentioned filtration container, after filtering the sample water by suction filtration or the like, the microbial disruption liquid is added from the sample water inlet 42 of the outer cylinder 41 so that the space between the microfilter 45 and the outer cylinder 41 is increased. It is possible to store the disrupted liquid of microorganisms. Hereinafter, in the present specification, the gap between the microfilter 45 and the outer cylinder 41, which is capable of storing the disrupted microorganism liquid as described above, is referred to as the disrupted microorganism reservoir 46.

FIG. 1 shows a block diagram of the microorganism measuring apparatus according to this embodiment.

In the figure, 1 is a sample water tank, 9 is a sample water transport pipe for connecting the sample water tank 1 and the electromagnetic valve 6, and the sample water transport pipe 9 has an electromagnetic valve 2, a flowmeter 3, and a sample. A pump 4 and a pressure gauge 5, which are water inflow means, are provided. By driving the pump 4, the sample water can be injected into the filter cartridge 9 through the electromagnetic valve 6 and the injection pipe 12.

At this time, the flow rate of the sample water is changed by the flow meter 3.
Further, the pressure gauge 5 can measure the liquid pressure of each sample water.

By switching the electromagnetic valve 2, the transport of the sample water is stopped and the sample water transport pipe 9
It is also possible to open the electromagnetic valve side of and to bring the inside of the sample water transport pipe 9 to normal pressure.

Reference numeral 8 denotes a microbial crushed liquid tank, 10 denotes a microbial crushed liquid transport pipe connecting the microbial crushed liquid tank 8 and the electromagnetic valve 6, and the microbial crushed liquid transport pipe 10 is a pump as a microbial crushed liquid injection means. 7 is provided. By driving this pump 7, the microbial disruption liquid tank 8
The microorganism crushed liquid therein can be injected into the filter cartridge 15 through the electromagnetic valve 6 and the injection pipe 12.

Further, the injection pipe 12 is provided with an electromagnetic valve 14 and a waste liquid pipe 13. By switching the electromagnetic valve 14, the sample water or the microbial disruption liquid transported to the injection pipe 12 is discharged to the waste liquid pipe 13 or a filter. Cartridge 15
It is configured to be transported to.

This filter cartridge 15 is set in advance in a position where ultrasonic crushing can be performed in the ultrasonic crusher, and a position where the sample water and the microbial disruption liquid can be supplied by the injection pipe 12 through the sample water inlet 42. It is installed in.

Further, in this embodiment, a through hole is provided in the sample water outflow portion of the filter cartridge 15, and the microbial disruption liquid collecting pipe 17 having the electromagnetic valve 18 and the pump 19 is passed through this through hole. At this time, the sample water and the like do not leak from the gap between the through hole and the sample water sampling tube.

By releasing the electromagnetic valve 18 and driving the pump 19, the microbial disrupted liquid stored in the microbial disrupted liquid storage portion 46 in the filter cartridge 15 is removed from the EIA.
It is configured so that it can be transported to the device 20.

The injection tube 12 is connected to the filter cartridge 1
5 is extended to the inside of the microbial crushed liquid storage portion 46 to enable collection of the microbial crushed liquid, and a branch pipe is provided in the injection pipe 12, and this branched pipe electromagnetic valve and pump are provided to collect the microbial crushed liquid in the EIA device 20. It may be configured to be transported to. In this case, it is not necessary to provide the through hole in the filter cartridge 15.

The filtered water outlet 43 of the filter cartridge 15 is provided with a filtered water transport pipe 21 having an electromagnetic valve 22 and an electromagnetic valve 23.

The electromagnetic valve 23 is provided with a transport pipe 27 and a pump 24. By switching the electromagnetic valve 23, the filtrate filtered by the filter cartridge 15 is transported to the tank 25 through the transport pipe 27. It is configured to be possible.

In the above apparatus, the pump, electromagnetic valve and the like are controlled by the control device, and the microorganisms can be automatically measured. The control contents are shown below.

In the initial state, each valve is set to the following state.

Valve 2 ... Closes the pipe 32 side and opens the sample water transport pipe 9 side.

Valve 6: The microbial disruption liquid transport pipe 10 side is closed and the sample water transport pipe 9 side and the injection pipe 12 side are opened.

Valve 14 ... The waste liquid pipe 13 side is closed and the injection pipe 12 side is opened.

Valve 18 ... Close Valve 22 ... Open.

Valve 23: The side of the transport pipe 33 is closed and the side of the transport pipe 27 and the side of the filtered water transport pipe 21 are opened.

The valves 26, 31 ... Are closed.

Further, all the pumps are put in a waiting state for operation.

1. The sample water is supplied to the sample water tank 1 by the water sampling device 11.

2. The sample water is sent from the sample water tank 1 to the filter cartridge 46 by driving the pump 4, and the sample water is filtered by this. In this embodiment, since the valve is initially set as described above, the sample water flows into the filter cartridge 15 through the sample water transport pipe 9 and the injection pipe 12 and is filtered under pressure in this filter cartridge. ..

As a result, the microorganisms in the sample water remain as a residue on the filter surface. The filtered water is discharged through the filtered water transport pipe 21 and the transport pipe 27.

3. The inflow amount of the sample water is measured by the flowmeter 3, and the pump 4 is stopped when the inflow amount of the sample water reaches a preset integrated amount.

Alternatively, the pressure of the sample water is measured by the pressure gauge 5, and when the measured value reaches a preset value by the controller, it is judged that the filter is clogged and the pump 4
Can also be stopped.

In this case, the flow rate integrated value up to that point is stored.

4. The electromagnetic valve 2 is switched to open the suction side of the pump 4 to the atmosphere (close the sample water tank side of the sample water transport pipe 9 and open the pipe 32 side). Next, the sample water remaining in the filter cartridge is pushed out to complete the filtration.

For example, the pump 4 is restarted with the suction side of the pump 4 open to the atmosphere, and the sample water transport pipe 9
Also, the sample water can be pushed out by a method of sending air into the filter cartridge 15 through the injection pipe 12 and performing pressure filtration. At this time, the air pressure can be detected by the pressure gauge 5, and the end of filtration can be detected based on this value to perform control.

5. After completing the filtration, the pump 4 is stopped again, the electromagnetic valve is switched to 6, the sample water transport pipe 9 side is closed, and the microbial disruption liquid transport pipe 10 side and the injection pipe 12 side are opened.

Further, after the electromagnetic valve 22 is closed, the pump 7 is driven so that a certain amount of the microbial disruption liquid is passed through the microbial disruption liquid transport pipe 10 and the injection pipe 12 to the filter cartridge 9
Inject.

As described above, the crushed liquid of microorganisms is not filtered under normal pressure, and thus the crushed liquid of microorganisms is stored in the crushed liquid storing portion 46 in the filter cartridge 15. At this time, if the transport amount of the crushed microbial liquid is large, the crushed microbial liquid is filtered under pressure by the pump 7, so the injection amount of the crushed microorganism liquid is set to an amount that does not cause the above-mentioned pressure filtration.

6. The ultrasonic crusher 16 is driven to crush the microorganisms in the filter.

7. Open the electromagnetic valve 18 and pump 19.
Is driven to suck the microbial crushed liquid in the microbial crushed liquid storage section 46. As a result, it is possible to send a fixed amount of the disrupted microbial solution that has undergone ultrasonic disruption to the EIA measuring device.

8. The electromagnetic valve 22 is opened. Further, the electromagnetic valve 14 is switched to close the electromagnetic valve 6 side of the injection pipe 12, and both the filter cartridge 15 side and the waste liquid pipe 13 side of the injection pipe 12 are opened.

Further, by switching the valve 23, the transport pipe 2
7 side is closed, and both the transportation pipe 33 side and the filtered water transportation pipe 21 side are opened.

After that, the pump 24 is driven to send the wash water in the wash water tank 25 into the filter cartridge 15 through the transport pipe 33 and the filtered water transport pipe 21 to backwash the filter in the filter cartridge.

By backwashing the filter, the filtered washing water is discharged as waste liquid through the injection pipe 12 and the waste liquid pipe.

As a result, the residue (microorganisms, microbial disruption liquid, etc.) in the filter cartridge is filtered, and the state where the microorganisms can be filtered again is restored.

9. The concentration of microorganisms and the like are measured by an EIA measuring device. At this time, the concentration ratio of the microorganism is calculated from the amount of the sample water in the item 3 and the amount of the buffer solution in the item 5,
It is transmitted as data of measurement in EIA. Thereby, the amount of microorganisms in the original sample water can be accurately measured.

The above operation is repeated. This operation can be easily repeated by controlling the pumps, valves and the like using an existing sequencer or computer.

In the third paragraph, the level of the filtrate was measured using a flow meter, but as shown in parentheses in FIG. 1, a water receiving tank 29 having a level meter 28 is provided, and this water receiving tank 29 is provided. Measure the water quality in the level meter 19,
The pump 4 may be controlled based on this value.

By carrying out the measurement based on the above control by the apparatus constructed as described above, it is possible to automatically, easily and accurately measure the microorganisms.

Therefore, particularly in a wastewater treatment plant or the like, it is possible to grasp the change in water quality in real time, which greatly contributes to water quality management and the like.

In FIG. 1, pump 4 and pump 7
However, these pumps may be provided between the electromagnetic valve 6 and the filter cartridge 9. At this time, one pump can be substituted for these pumps by switching and controlling the electromagnetic valve 6.

Furthermore, although the crushed liquid side of microorganisms is closed and the pumps are provided on the pumping side by one pump, these pumps may be provided on the suction side of the filter cartridge 9.
At this time, the pump 4 and the pump 7 may be replaced by one pump.

[0092]

EFFECTS OF THE INVENTION Since the present invention constitutes the microorganism measuring apparatus as described above, the following effects can be obtained.

A. The operation of collecting the microorganisms can be performed easily and in a short time, and the recovery rate of the microorganisms can be increased and stabilized.

B. A minute amount of microorganisms can be automatically and continuously measured, and concentration and crushing of microorganisms can be automatically and continuously performed.

C. The sample water is filtered using a filter cartridge having a gap, and after the filtration is completed, the microbial disruption liquid is stored in this gap to bring the microorganisms captured by the filter into contact with the microbial disruption liquid. There is.

Therefore, conventionally, after the filtration of the sample solution is completed, it is necessary to prepare in advance a container containing the microorganism crushing solution and the microorganism crushing solution, and to put the microbial crushing filter into the microorganism crushing solution. However, such a complicated and labor-intensive operation is unnecessary. Therefore, human resources can be efficiently used, which is economically advantageous. d. By using a control device, etc., microorganisms in the sample water can be measured automatically and continuously.
It is economically advantageous. For example, it is easy to continuously monitor the quality change of treated water in a sewage treatment plant.

E. It is also possible to easily construct a feedback system in which the number of E. coli is a target value in controlling the amount of chlorine injection in a chlorine sterilization facility.

[Brief description of drawings]

FIG. 1 is an explanatory diagram of a microorganism measuring apparatus according to an embodiment of the present invention.

FIG. 2 is an explanatory diagram of a filter cartridge according to an embodiment of the present invention.

FIG. 3 is an explanatory view of a microorganism filtration device according to a conventional example.

FIG. 4 is an explanatory view of a microorganism filtration device according to a conventional example.

[Explanation of symbols]

 1 ... Sample water tank, 2 ... Electromagnetic valve 3 ... Flowmeter 4 ... Pump 5 ... Pressure gauge 6 ... Electromagnetic valve 7 ... Pump 8 ... Microbial disrupted liquid tank 9 ... Sample water transport pipe 10 ... Microbial disrupted liquid transport pipe 11 ... Water device 12 ... injection pipe 13 ... waste liquid pipe 14 ... electromagnetic valve 15 ... filter cartridge 16 ... microorganism crushing device 17 ... microorganism crushed liquid collecting pipe 18 ... electromagnetic valve 19 ... pump 20 ... EIA device 21 ... filtered water transport pipe 22 ... electromagnetic Valve 23 ... Electromagnetic valve 24 ... Pump 25 ... Tank 26 ... Valve 27 ... Transport pipe 28 ... Level meter 29 ... Water receiving tank 32 ... Pipe 33 ... Transport pipe 41 ... Outer cylinder 42 ... Sample water inlet 43 ... Filtered water outlet 44 ... Filter support part 45 ... Micro filter 46 ... Microbial disrupted liquid storage part

Claims (2)

[Claims] 1. A filter cartridge having a microbial capture filter for partitioning the inside of an outer cylinder into a sample water inlet side and a sample water outlet side inside an outer cylinder provided with a sample water inlet and a filtered water outlet, and a sample water Sample water supply means for supplying, sample water inflow means for injecting sample water obtained from the sample water supply means into the filter cartridge, and forced filtration of sample water flowing into the filter cartridge to obtain the microorganism-trapping filter. Forced filtration means for separating microorganisms and filtrates captured as a residue on top, microbial crushed liquid supply means for supplying microbial crushed liquid, and after completion of filtration of the sample water, from the microbial crushed liquid supply means The resulting microorganism disruption liquid is injected into the gap between the microorganism trapping filter in the filter cartridge and the sample water outflow side of the outer cylinder to destroy the microorganism. A liquid injecting means, a filter cartridge having a position capable of crushing microorganisms in advance, a microbial crushing means for crushing the microorganisms after the injection of the microbial crushed liquid into the gap portion, a microorganism measuring means, and A microbial disruption liquid transporting means for transporting the microbial disruption liquid injected into the gap to the microorganism measuring means after the disruption of the microorganisms is completed. 2. The microorganism measuring device according to claim 1, wherein the microorganism measuring device removes residual microorganism disrupting liquid in the filter cartridge after the microorganism disrupting liquid is transported to the microorganism measuring means. A removing means, a cleaning water supplying means, and a cleaning water obtained from the cleaning water supplying means are caused to flow into the filter cartridge to clean the microorganism-trapping filter, and thereby after cleaning the microorganism-trapping filter. A microorganism measuring device, comprising: a microorganism capturing filter cleaning means capable of repeatedly measuring the sample water obtained from the sample water supplying means.