JPH05163115A - Beautifying and whitening cosmetic - Google Patents

Abstract

PURPOSE:To obtain a beautifying and whitening cosmetic having suppressing effects on melanogenesis, effects on rapid lightening of the pigmentation color on the skin and suppressing effects on inflammation with ultraviolet rays without any skin irritation. CONSTITUTION:A beautifying and whitening cosmetic is obtained by blending (A) a melilotus extract with (B) a deproteinized blood serum. The amounts of the ingredients based on the beautifying and whitening cosmetic are 0.01-5.0wt.% ingredient (A) and 0.001-3.0wt.% ingredient (B). This beatifying and whitening cosmetic is excellent in beautifying and whitening effects and suppressing effects on inflammation. Furthermore, the ingredient (A) is extracted from a stem, a leaf, a root or the whole herb of Melilotus officinalis (L.) Lam. belonging to the subfamily Caesalpinioideae of the family Leguminosae and contains melilotoside, melilotic acid, coumarin, their related substances, etc. The ingredient (B) can be produced by adding acetone to blood of a calf, etc., free of cellulose, then adding a 1:1 mixture of acetone with ether, centrifuging the mixture, subsequently drying the resultant substance, adding distilled water and trypsin, fermenting the obtained mixture at pH 8 and 37 deg.C, then dialyzing the fermentation liquor with cellophane and concentrating the dialyzate.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a whitening cosmetic composition which has the effect of rapidly lightening dark-skinned skin and the effect of preventing skin irritation due to ultraviolet rays and is excellent in skin safety.

[0002]

2. Description of the Prior Art Ultraviolet rays change the color tone of skin and turn it black. This blackening is caused by the overproduction of melanin produced in melanocytes and delivered to epidermal cells, and melanin is produced by the oxidation of tyrosine.

[0003] Conventionally, in order to prevent skin blackening, stains and freckles and maintain the original white skin, vitamin C salts and fatty acid derivatives which prevent this oxidation, hydroquinone monobenzyl ether, hydrogen peroxide, etc. have been added. Whitening cosmetics have been proposed.

However, when a vitamin C derivative is incorporated into these whitening cosmetics, it is not always sufficient in terms of whitening effect and anti-inflammatory effect. On the other hand, when hydroquinone monobenzyl ether or the like is blended in the whitening cosmetic composition, it has the effect of lightening dark skin, but it has a drawback in that it has a problem in terms of skin safety. As described above, it is extremely difficult to obtain a whitening cosmetic composition which is excellent in whitening effect and inflammation suppressing effect and has high skin safety.

In view of the above situation, the present inventor has conducted earnest studies as an improvement of the drawbacks of the prior art, and as a result, the combination of melilot extract and serum deproteinization has a whitening effect and an inflammation suppressing effect. The inventors have found that the whitening cosmetic composition satisfies the conditions of being excellent and having high skin safety, and completed the present invention.

That is, an object of the present invention is to provide a whitening cosmetic composition which is excellent in whitening effect and inflammation suppressing effect and has high skin safety.

[0007]

In order to achieve the above object, the whitening cosmetic composition of the present invention has the following constitution. That is, the present invention is a whitening cosmetic characterized in that it contains a melilot extract and a serum deproteinization product.

The melilot extract used in the whitening cosmetic composition of the present invention is a component extracted from the stems, leaves, whole plants or roots of Scutellaria barbacarina belonging to the subfamily Scarabaeidae of the leguminous family. Extracted components include melilot side, melilot acid, coumarin and related substances. As the extraction solvent, hot water, hydrous alcohol,
Alcohols, acetone, ethyl acetate, chloroform and the like can be used.

The serum deproteinized product used in the whitening cosmetic composition of the present invention can be obtained, for example, by the following method. That is, the fibrin of blood such as a cow as a raw material is removed, acetone is added, and then a mixture of acetone: ether = 1: 1 is added, followed by centrifugation and drying. Distilled water and trypsin are added to the dried product to ferment at pH 8 and 37 ° C. After fermentation, cellophane dialysis is carried out and concentrated to obtain an extract. The obtained extract is a yellowish transparent liquid and contains 4 to 5% of solids. Further, these extracts can be purchased as Solcoceryl (manufactured by Solco Basel) or S-R71 (manufactured by Botoger).

The blending amount of the melilot extract in the whitening cosmetic composition of the present invention is preferably 0.0 based on the total amount.
1 to 5.0% by weight (hereinafter referred to as wt%).

The amount of serum deproteinized protein in the whitening cosmetic composition of the present invention is a dry solid amount, preferably 0.001 to 3.0 wt% based on the total amount.

The blending amount of the melilot extract is 0.01% by weight.
If the amount of the serum deprotein is less than 0.001w%, the effect of the present invention is not sufficient. Even if the amount of the melilot extract exceeds 5.0w% and the amount of the serum deprotein exceeds 3.0w%, respectively. , I cannot hope to improve the effect commensurate with the increase,
The feel during use tends to be poor and it is difficult to hold individual dosage forms.

The whitening cosmetic composition of the present invention can be formulated into lotions, emulsions, creams, packs and the like according to a conventional method.

In the whitening cosmetic composition of the present invention, dyes, fragrances, preservatives, surfactants, pigments and the like can be appropriately added within the range to achieve the object of the present invention.

[0015]

EXAMPLES The present invention will be described in detail below based on examples and comparative examples.

Each test of (1) tyrosinase activity inhibition test, (2) skin color lightness recovery test, (3) whitening practical test, (4) ultraviolet erythema inhibition test, and (5) optical patch test described in Examples. The law is as follows.

(1) Tyrosinase activity inhibition test 0.3 m per 1 ml of Macklebein buffer (pH 6.8)
A sample solution of each concentration is added to the tyrosine solution of g / ml concentration, and pre-incubation is performed at 37 ° C for 10 minutes. 1m to this
0.1 ml of tyrosinase (manufactured by Sigma) at a concentration of g / ml
Was added and heated at 37 ° C. for 15 minutes, and then the absorbance (A) was measured at a wavelength of 475 nm using a spectrophotometer. On the other hand, the absorbance (B) of 0.1 ml of buffer solution added in place of tyrosinase, 0.1% buffer solution in place of the sample solution.
The absorbance (C) of the solution added with ml and the absorbance (D) of the sample solution added with 0.2 ml of the buffer solution instead of tyrosinase were measured, and the inhibition rate (%) was calculated according to the following formula.

Inhibition rate (%) = (A−B) / (C−D) × 100

(2) Skin color lightness recovery test The skin of the upper arm of 20 test subjects was irradiated with the minimum amount of erythema of UV rays in the UVA and UVB regions for 3 consecutive days. The reference brightness (V0 value, V0 'value) of the skin of the applied part was measured. Then, apply 3 times a day for 4 weeks continuously and start irradiation 1.
After 2 or 4 weeks, the skin lightness (Vn value, Vn 'value) of the sample cream-applied part and the base cream-applied part was measured,
The skin color recovery was evaluated according to the criteria shown in Table 1.

The brightness of the skin (V value of Munsell display system)
Was calculated from the X, Y, and Z values obtained by measurement with a high-speed spectrocolorimeter. The evaluation is shown by the average value of the evaluation points of 20 test subjects after 4 weeks.

[0021]

[Table 1]

(3) Practical Whitening Test A left forearm flexion side part of the forearm flexion side skin of 20 test subjects exposed to sunlight in summer for 3 hours (1.5 hours a day for 2 days) The sample cream and the base cream were applied once daily in the morning and evening for 13 weeks on the skin from the day of exposure to sunlight and from the day 7 of the day of exposure to sunlight to the skin on the right forearm flexor side.

(4) UV Erythema Suppression Test U was applied to the dorsal skin of 10 hair-removed Hartley guinea pigs.
Irradiation with two times the minimum erythema dose of ultraviolet rays in the VB region is performed at two locations each. The sample was applied 24 hours before and immediately after irradiation, the sample application site and the base application site were set, and 24 hours later, the erythema state was evaluated according to the criteria in Table 2.

[0024]

[Table 2]

(5) Optical patch test After 25 hours of closed patch was performed for 24 hours using a patch plate having a diameter of 1.0 cm, which was prepared by applying 0.05 g of the sample to the skin on the flexion side of the forearm of 25 subjects, sunlight in summer was applied. 6 hours (3 a day
For 2 days).

The evaluation was performed according to the criteria shown in Table 3 for subject 2
The skin condition of 5 persons was evaluated and judged. Judgment result is irradiation 2
After 4 hours, the number of people was (±) or more.

[0027]

[Table 3]

Examples 1 to 3 and Comparative Examples 1 to 4 Two-Phase Lotion In the raw material composition of Table 4, the active ingredients are blended as shown in Table 4 to prepare a two-phase lotion, and the above-mentioned various tests are conducted. Was carried out.

[0029]

[Table 4]

(1) Preparation method The components B and C shown in Table 5 are uniformly dissolved in the component D, and then the components A and D are uniformly mixed with stirring and dispersed, and then filled in a container. At the time of use, the contents should be evenly dispersed by shaking.

[0031]

[Table 5]

(2) Characteristics Table 5 shows the results of various tests. As shown in Table 5, Comparative Example 1 did not show good results in various tests.

The whitening cosmetics of the present invention of Examples 1 to 3 clearly showed good results in all the tests, and no skin irritation occurred in the tests on human skin.

Examples 4 to 7 and Comparative Examples 5 to 8 Skin Cream The skin cream was prepared by blending the active ingredients in the raw material composition shown in Table 6 as shown in Table 7, and the above-mentioned various tests were carried out.

[0035]

[Table 6]

[0036]

[Table 7]

(1) Preparation method Component B shown in Table 7 is mixed with component D, and component A and component D are uniformly heated and dissolved to bring the temperature to 80 ° C. Next, the component D is poured into the component A, mixed by stirring, cooled, while stirring, and the component C is added at about 50 ° C. and cooled to 30 ° C.

The results of various tests are shown in Table 7. As shown in Table 7, Examples 4 to 6 show clearly good results in all of the tests, good results in the tests with human skin, and no skin irritation in the tests with human skin. Did not happen.

[0039]

As described above, the present invention is excellent in the effect of suppressing the production of melanin pigment, the effect of rapidly lightening skin pigmentation, and the effect of suppressing skin inflammation due to ultraviolet rays.
It is obvious to provide a useful whitening cosmetic which does not cause skin irritation.

Claims (1)

[Claims] 1. A whitening cosmetic composition, which comprises a melilot extract and a serum deproteinization product.