JPH05149955A - Automatic chemical analysis device - Google Patents

Abstract

PURPOSE:To check a cross contamination in an automatic chemical analysis device automatically. CONSTITUTION:A processing part 32 automatically issues a work sheet for searching for a combination of items which induce cross contamination of a reaction container according to the number of specified items and a work sheet for searching for a combination of items which induce cross contamination of a reagent-pouring mechanism. A data operation part 34 performs measurement according to the issued work sheets and then compares measurement results for each item with a reference value, thus enabling presence or absence of cross contamination to be judged. A file part 36 retains the judgment result as a cross contamination data at the time of normal measurement.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an automatic chemical analyzer for measuring the concentration or activity value of a target component in a sample containing multiple components such as blood and urine.

[0002]

2. Description of the Related Art The analysis section of such an automatic chemical analyzer is equipped with a sample injection mechanism for dispensing a sample into a reaction container along with an annular reaction line in which the reaction containers are arranged in a line and conveyed. At least a reagent injection mechanism for dispensing a reagent into the injected reaction container, an absorptiometer for measuring the absorbance of the reaction solution in the reaction container, and a cleaning mechanism for cleaning the reaction container are provided. In such a so-called single-multi type automatic analyzer, the reagent dispenser and the reaction container are washed and repeatedly used, but even if the reagent and the reaction solution attached to the reagent dispenser and the reaction container are washed. Can not be completely removed, and may cause cross contamination.
Cross contamination depends on the type of items and reagents.
Since it may or may not occur, each facility sets the reagents actually used in the automatic chemical analyzer to check for cross contamination.

[0003]

Since the automatic chemical analyzer is a random selection device, it is necessary to check the combination of all items. Due to the relationship between the number of items and the number of reaction vessels, the logic to create a worksheet for checking cross contamination is not the same, so request registration is performed in the same manner as general routine analysis. , Mistakes are easy to make. Moreover, there are inconveniences that it takes time to register the request and it is necessary to prepare many kinds of samples. An object of the present invention is to automatically check cross contamination in an automatic chemical analyzer.

[0004]

According to the present invention, a worksheet is automatically issued when the number of items is designated, cross contamination is automatically checked according to the worksheet, and the result is measured at the time of normal measurement. It is retained as the cross contamination data of. Therefore, the present invention is configured as shown in FIG. Three
Reference numeral 0 is an analysis unit of the automatic analyzer, which includes a reaction line, a sample injection mechanism, a reagent injection mechanism, an absorptiometer, a cleaning mechanism, and the like. Control unit Control unit 31 controls the operation of each unit of the analysis unit, calculates the concentration or activity value of the sample by the absorbance from the spectrophotometer, and also cross-contaminates the reaction container according to the designated number of items. A processing unit 3 for automatically issuing one or both of a worksheet for searching for a combination of items to be generated and a worksheet for searching for a combination of items to cause cross contamination of the reagent injection mechanism.
2 、 Measure according to the published worksheet,
A data calculation unit 34 for comparing the measurement result of each item with a reference value to determine the presence or absence of cross contamination, and a file unit 36 for holding the determination result as cross contamination data during normal measurement. ..

[0005]

When the operation mode for checking the cross contamination is designated, the processing section 32 automatically creates a check worksheet according to the designated number of items. The data calculator 34 analyzes according to the worksheet. When checking the cross contamination of the reaction container, the same sample is divided into at least two rounds of the reaction line for analysis. The measurement data is processed item by item, compared with a reference value, and if it deviates from the reference value, it is determined to be a combination with cross contamination and stored in the file unit 36. The data stored in the file unit 36 is used to prevent cross contamination during normal analysis.

[0006]

EXAMPLE A worksheet for checking cross contamination of a reaction container will be described. Consider a case where m kinds of items are applied to an automatic analyzer having N reaction vessels. When the items are T ₁ , T ₂ , ... Tm, the combinations of items to be checked are T ₁ → T ₁ , T ₁ → T ₂ , ..., T ₁
→ Tm, T ₂ → T ₁ , T ₂ → T ₂ , ..., T ₂ → Tm, Tm
→ T ₁ , Tm → T ₂ , ..., Tm → Tm m ² pieces are considered. The number of combinations is m (m−1), except for combinations with itself (T ₁ → T _1, etc.).

Consider a method of creating a worksheet for the case of m = 10. First, it is basically measured with the same number of reaction vessels as m. That is, as shown in FIG. 2, the reaction line is equipped with 10 reaction vessels, numbered 1-10. On the first lap, the items T _{1 to} T ₁₀ are measured in the reaction vessels 1 to 10. An example of the worksheet is shown in FIG. However, in the diagram of the worksheet, T indicating an item is omitted, and only the numeral of the item number is shown. On the second lap, place item T ₂ under item T _{1 on} the first lap and move T ₃ to the right,
...... Fill in with T ₁₀ , and in the 10th reaction vessel, item T
Measure ₁ . 3 lap puts the item T ₃ under the second round of item T ₁ (10 th reaction vessel), T ₄ back to the first reaction vessel 1, and _{...... T 10, T 1, T} 2 Fill to the right. That is, each time the measurement has completed one round, the number under the item T ₁ is moved up and set to the first, and thereafter, according to the order of the reaction vessels in FIG.
Fill the remaining reaction vessels with items. If this is continued up to the 10th lap, all combinations of 10 × (10-1) = 90 are obtained. Regardless of the value of m, a basic worksheet is completed by the same procedure, and it is necessary to measure m rounds.

When the number N of reaction vessels is larger than the number m of items, a basic work sheet is checked, and N-m empty reaction vessels are formed for one round, which wastes time.
Therefore, consider measuring m items a plurality of times for each round. The number of times the measurement can be performed for each rotation is determined by the quotient a of N ÷ m (the remainder is b, that is, N = a × m + b). Now, if 10 items are checked with an automatic chemical analyzer having 30 reaction vessels, the quotient a is 3 (= 30 ÷ 1).
It becomes 0), and 10 items should be measured 3 times for each round. By the way, the basic worksheet measures N
o. I had to measure 9 laps, 1 → 2, 2 → 3, …… 9 → 10, but when dividing it, 3 laps (9 ÷ 3
= 3) needs to be measured, the reaction container numbers 1 to 10 are the measurement numbers of the basic worksheet. 1-4, reaction vessel number 1
1 to 20 are measurement Nos. 4 to 7, reaction vessel numbers 21 to 30
Is the measurement number. By setting the number to be 7 to 10, the worksheet shown in FIG.

In the worksheet of FIG. 3 (B), the reaction container Nos. 1-10 No. 4 and N of reaction vessel numbers 11 to 20
o. No. 1 of the reaction container numbers 11 to 20. No. 4 and reaction container numbers 21 to 30. The order is the same as that of 1. 3C shows the measurement No. of the basic worksheet. 3 is the measurement No. of the reaction container numbers 11 to 20. No. 2 of the measurement number of the basic worksheet. 4 is the measurement No. 4 of the reaction container numbers 21-30. It was created by fitting it to 2.

Now, the items T ₁ → T ₂ → ... T ₁₀ → T ₁ → ...
Cross-contamination does not occur in the reagent dispenser when measuring in the order of. However, FIG. 3 (B)
When the same item is measured a plurality of times within one round of the reaction line as in (C) or (C), an order other than the above order, for example, T ₁₀ → T ₇ or T ₆ → T _{2 in} FIG. Combinations of different orders can also occur. Therefore, if a step of injecting a detergent or water as a dummy into the reaction container is added during such a combination, it is convenient because cross contamination in the reagent dispenser can be prevented. Therefore, when the number of reaction vessels is not an integral multiple of the number of items, dummy analysis may be performed using the remainder of the number of reaction vessels divided by the number of items. For example, when there are 32 reaction vessels, a worksheet as shown in FIG. Reaction container numbers 11 and 22 are dummy. In the dummy analysis, water is dispensed instead of the sample, and water or detergent is suctioned instead of the reagent and dispensed into the reaction container or the cleaning pot.

Consider a case where there are 29 reaction vessels in the reaction line. Considering a worksheet in which only 20 reaction vessels are used in one round of the reaction line, it becomes as shown in FIG. 4 (B). This worksheet is simple to sort,
It takes time only for empty reaction vessels. However, this empty reaction container can be placed between a group of 10 items and a group of the next 10 items for dummy dispensing.

Considering the case where 29 reaction vessels are used for one round of the reaction line, the worksheet becomes as shown in FIG. 4 (C). This worksheet requires less measurement time, but the logic is complex. The number of measurements is 110 and (C) in FIG.
Then it is 119. When considering the number of tests, a dummy may be inserted for every 10 items and the value may be set to +1 and 11 items may be considered as a unit.

Samples are dispensed sequentially according to such a worksheet (since the same sample is measured, the sample transfer means is stopped or a sample suction position dedicated to this measurement mode is provided)
Then, the reagent is dispensed. The presence or absence of cross contamination is judged based on the obtained results. For example, a target value and an allowable value are registered as reference values, and if they deviate from that, they are stored as a combination with cross contamination.If such a combination occurs during normal analysis, the analysis order is changed or detergent is distributed in the reaction container. It is used for ordering and skipping.

Next, the check for cross contamination of the reagent dispenser will be described. The worksheet for measuring 10 items is as shown in FIG. The numbers in the table represent the order of measurement. As a result of the measurement using the worksheet of FIG. 5, for example, if the 6th data is significantly different from the other 2nd, 4th, 8th, ... After the measurement, that is, the measurement of T ₃ → T ₁ , it is determined that cross contamination has occurred. The fifth data is 23
No. 39, 40, and so on, it is judged that cross contamination has occurred in the measurement from T _{1 to} T ₃ .

Ten data are obtained for each of the items T ₁ and T ₁₀ , and 11 data are obtained for each of the items T _{2 to} T ₉ . If there are the same combinations, for example, T ₁ →
T ₂ appears in the 3rd measurement and the 21st measurement, and in that case, either data may be used to determine the cross contamination. Analysis is performed according to the worksheet of FIG. 5 as in the case of reaction vessel contamination. In this case, dummy dispensing is not performed. However, during this analysis, if a combination that is judged to have reaction vessel contamination occurs when the reaction vessel enters the second lap, the reagent dispenser stops all movements and only the reaction vessel Will be sent in the air.

Whether the cross-contamination check of the reaction container or the cross-contamination check of the reagent dispenser is performed, the output as shown in FIG. Is. In the example of the display, if you distinguish between the reaction container and reagent dispenser, item name, target value, allowable value, actual measured value after itself, measured value after other items Good. H or L marks may be added to the measured values that exceed the allowable value.

The measurement of the same request may be repeated twice in order to increase the reliability of the data. In this case, both of the reaction vessel contamination and the reagent dispenser cross contamination can use the value obtained in combination with itself as the target value. At the time of checking the cross contamination of the reaction vessel, the analysis is started from a clean state of the reaction vessel, so the value of the first round may be used as the target value. It is also possible to create a worksheet that can handle cases where a new item is added to a place where an already checked item exists.

FIG. 7 shows an example of the analysis unit of the automatic chemical analyzer to which the present invention is applied. Reference numeral 2 is a reaction disk, and reaction vessels 4 also serving as cuvettes are arranged in a row along the cuvette roller 3 to form an annular reaction line 5. A sample injection mechanism 6 is arranged along the reaction line 5 for injecting a sample specimen into the reaction container. In the sample injection mechanism 6, the sample cups 7 are arranged along the circumference of the sampling table 8, and the sample dispenser 9 is arranged to dispense the sample from the sample cup 7 at the sample suction and collection position 13. .. A dispensing nozzle 10 is provided at the tip of the sample dispenser 9, and the dispensing nozzle 10 moves along a moving path 11 between a reaction container at a sample dispensing position 14 and a sample cup at a sample aspirating and sampling position 13. Moving. A cleaning pot 12 is provided on the moving path 11 so that the nozzle 10 can be cleaned.

A reagent injection mechanism 16 is arranged along the reaction line 5 in order to inject the reagent into the reaction container into which the sample has been dispensed. In the reagent injection mechanism 16, a reagent container 17 is arranged along the circumference of the reagent tray 18, and a reagent dispenser 19 is arranged to dispense a reagent from the reagent container 17 at the reagent suction and collection position 23. ..
A dispensing nozzle 20 is provided at the tip of the reagent dispenser 19, and the dispensing nozzle 20 runs between the reaction container at the reagent dispensing position 24 and the reagent container at the reagent aspirating and sampling position 23 along the movement path 21. Moving. A cleaning pot 22 is arranged on the moving path 21 so that the nozzle 20 can be cleaned. A washing and dehydrator 26 is further arranged on the reaction line 5,
An absorptiometer 27 corresponding to the measurement unit along the reaction line 5
Is also arranged. The reaction line 5 rotates intermittently in the direction of arrow 15.

[0020]

According to the present invention, a worksheet for checking cross contamination is automatically issued, and measurement is automatically performed in accordance with the worksheet, so that a request for cross contamination check can be made. No need to register, no mistakes. In addition, it is not necessary to prepare many check samples. Since the result of the check is automatically determined and stored in the file section, there is no need to organize the data. As described above, according to the present invention, the cross contamination check can be automated, and the operability is improved.

[Brief description of drawings]

FIG. 1 is a block diagram showing the present invention.

FIG. 2 is a schematic view showing a reaction line having 10 reaction vessels.

3A to 3C are diagrams showing examples of worksheets for checking the cross contamination of the reaction container when the number of items is 10. FIG.

FIGS. 4A to 4C are diagrams showing examples of worksheets for checking cross-contamination of reaction vessels when the number of items is 10 and the number of reaction vessels is other than an integral multiple thereof.

FIG. 5 is a diagram showing an example of a worksheet for checking cross contamination of a reagent dispenser.

FIG. 6 is a diagram showing an example of display of measurement results of cross contamination.

FIG. 7 is a schematic plan view showing an analysis unit of an automatic chemical analysis device to which the present invention is applied.

[Explanation of symbols]

 30 analysis unit 32 processing unit 34 data calculation unit 36 file unit

Claims (1)

[Claims] 1. A ring-shaped reaction line in which reaction vessels are arranged and conveyed in a line, a sample injection mechanism for dispensing a sample into the reaction vessel, a reagent injection mechanism for dispensing a reagent into the reaction vessel into which the sample has been injected, An analysis unit having at least an absorptiometer for measuring the absorbance of the reaction solution in the reaction container and a cleaning mechanism for cleaning the reaction container, and controlling the operation of each unit of the analysis unit,
It comprises a control unit for calculating the concentration or activity value of the sample by the absorbance from the spectrophotometer, the control unit further, a combination of items causing cross-contamination of the reaction container according to the number of items specified. Worksheet to search for a combination of items that cause cross-contamination of the reagent injection mechanism and a processing unit that automatically issues one or both of the worksheets to search for, and perform measurement according to the issued worksheets A data operation unit that compares the measurement result for each with a reference value to determine the presence or absence of cross contamination, and a file unit that holds the determination result as cross contamination data during normal measurement, Automatic chemical analyzer.