JPH05124910A - Sterilizing and disinfecting liquid and sterilizing and disinfecting material containing the same - Google Patents
Sterilizing and disinfecting liquid and sterilizing and disinfecting material containing the sameInfo
- Publication number
- JPH05124910A JPH05124910A JP3321575A JP32157591A JPH05124910A JP H05124910 A JPH05124910 A JP H05124910A JP 3321575 A JP3321575 A JP 3321575A JP 32157591 A JP32157591 A JP 32157591A JP H05124910 A JPH05124910 A JP H05124910A
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- JP
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- Prior art keywords
- sterilizing
- disinfecting
- solution
- benzalkonium chloride
- impregnated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Medicinal Preparation (AREA)
- Materials For Medical Uses (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】この発明は、殺菌消毒液及びこれ
を用いる殺菌消毒材に関する。更に詳しくは、塩化ベン
ザルコニウム（ＢＡＣ）、エタノール、及び水の三者を
含有する殺菌消毒液、及びこの消毒液を不織布に含浸さ
せた殺菌消毒材に関し、特に医療・臨床分野における手
指の殺菌・消毒用に利用されるものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a sterilizing and disinfecting liquid and a sterilizing and disinfecting material using the same. More specifically, it relates to a sterilizing and disinfecting solution containing benzalkonium chloride (BAC), ethanol, and water, and a sterilizing and disinfecting material in which a nonwoven fabric is impregnated with this disinfecting solution, and particularly to sterilization of fingers in medical and clinical fields. -It is used for disinfection.
【０００２】[0002]
【従来の技術と発明が解決しようとする課題】従来、医
療・臨床分野における殺菌消毒液として種々の薬剤が使
用されているが、中でも塩化ベンザルコニウムとエタノ
ールは、副作用（刺激性）が少なく、また経済性等の点
で優れているため、夫々単独で広く使用されている。し
かしながら、最近の医療・臨床分野においては、種々の
抗生物質に対する耐性菌が出現し、中でもメチシリン耐
性 Staphylococcus aureus （ＭＲＳＡ）の出現が関係
者を悩ませており、更に優れた殺菌消毒液の開発が望ま
れている。発明者等は永年これらの分野において、殺菌
消毒薬についての研究を続けてきたが、塩化ベンザルコ
ニウムとエタノールの混液は、殺菌消毒液として使用す
ると、意外にも相乗効果があり、夫々を単独で使用した
よりも殺菌効果が大きいことを知り本発明を完成した。
また、医療・臨床分野における殺菌消毒液は、予めガー
ゼ、脱脂綿等に含浸させた殺菌消毒材として使用される
場合が多い。すなわち、殺菌消毒液を含浸させた、ガー
ゼ、綿等により、手、指等を拭う（拭く）のが最も頻繁
に使用される使用形態である。しかしながら、殺菌消毒
液を含浸させたガーゼや脱脂綿等で皮膚（患部）等を拭
うと、殺菌消毒後の皮膚の表面に、前記ガーゼや綿等が
ほつれた繊維が残り易い。そして、これらの皮膚等の表
面に残った繊維は、汚染され易く、微生物（病原菌）の
繁殖場所となり、かつ殺菌も困難であるため困っていた
のである。そこで発明者等は、殺菌消毒液を含浸させる
基材について、種々検討した結果、ガーゼや脱脂綿等に
代えて、不織布が殺菌消毒後においても繊維が残らず、
殺菌効果も良好であることを知り本発明を完成した。更
に、最近の医療・臨床分野においては、種々の抗生物質
に対する耐性菌が出現し、中でもメチシリン耐性 Stap
hylococcus aureus （ＭＲＳＡ）の出現が関係者を悩ま
せている。Conventionally, various chemicals have been used as sterilizing and disinfecting solutions in the medical and clinical fields. Among them, benzalkonium chloride and ethanol have few side effects (irritation). Moreover, since they are excellent in terms of economy, they are widely used by themselves. However, in recent medical and clinical fields, resistant bacteria to various antibiotics have emerged, and among them, the emergence of methicillin-resistant Staphylococcus aureus (MRSA) has bothered the parties concerned, and the development of an even better bactericidal disinfectant solution. Is desired. The inventors have continued research on germicidal disinfectants in these fields for many years, but when a mixture of benzalkonium chloride and ethanol is used as a germicidal disinfectant, it has a surprising synergistic effect, and each of them is used alone. The present invention has been completed, knowing that the bactericidal effect is greater than that used in.
In addition, a sterilizing / disinfecting solution in the medical / clinical field is often used as a sterilizing / disinfecting material obtained by previously impregnating gauze or absorbent cotton. That is, the most frequently used form is to wipe (wipe) hands, fingers, etc. with gauze, cotton, etc. impregnated with a sterilizing solution. However, when the skin (affected area) or the like is wiped with gauze or absorbent cotton impregnated with a sterilizing / disinfecting solution, the fibers on which the gauze, cotton, etc. are frayed tend to remain on the surface of the skin after sterilization. The fibers remaining on the surface of the skin and the like are easily contaminated, become a breeding site for microorganisms (pathogenic bacteria), and are difficult to sterilize, which is a problem. Therefore, the inventors, for the substrate to be impregnated with a sterilizing and disinfecting solution, as a result of various studies, in place of gauze, absorbent cotton, etc., no fibers remain after sterilizing and disinfecting the nonwoven fabric,
The inventors have completed the present invention knowing that the bactericidal effect is also good. Furthermore, in recent medical and clinical fields, resistant bacteria to various antibiotics have emerged, among which methicillin-resistant Stap
The emergence of hylococcus aureus (MRSA) has plagued stakeholders.
【０００３】本願発明は以上の課題を解決するためにな
されたもので、次の請求項（１）〜（５）により構成さ
れている。 （１）塩化ベンザルコニウム（ＢＡＣ）、エタノール、
及び水の三者を含有することを特徴とする殺菌消毒液。 （２）塩化ベンザルコニウム（ＢＡＣ）、エタノール、
及び水の三者の比が、１〜５０：５０〜３０００：５０
０〜１００００である前記（１）に記載する殺菌消毒
液。 （３）塩化ベンザルコニウム（ＢＡＣ）、エタノール、
及び水の三者を含有する殺菌消毒液を不織布に含浸させ
たことを特徴とする殺菌消毒材。 （４）不織布の材質が綿を主材料とする前記（３）に記
載する殺菌消毒材。 （５）塩化ベンザルコニウム（ＢＡＣ）、エタノール、
及び水の三者の比が、１〜５０：５０〜３０００：５０
０〜１００００である殺菌消毒液を使用する前記（３）
及び前記（４）に記載する殺菌消毒材。The present invention has been made to solve the above problems and is constituted by the following claims (1) to (5). (1) benzalkonium chloride (BAC), ethanol,
And a sterilizing and disinfecting solution containing three of water. (2) benzalkonium chloride (BAC), ethanol,
And the ratio of water to water is 1 to 50:50 to 3000: 50.
The sterilizing and disinfecting solution according to (1), which is 0 to 10000. (3) benzalkonium chloride (BAC), ethanol,
A sterilizing and disinfecting material, characterized in that a nonwoven fabric is impregnated with a sterilizing and disinfecting solution containing water and water. (4) The sterilizing and disinfecting material according to (3), wherein the material of the nonwoven fabric is mainly cotton. (5) benzalkonium chloride (BAC), ethanol,
And the ratio of water to water is 1 to 50:50 to 3000: 50.
The above (3) using a sterilizing solution of 0 to 10000
And the sterilizing material according to (4) above.
【０００４】本願発明に使用される不織布は、綿（コッ
トン）を多量に含むものが、保湿性、肌触り、ふき取り
性等が良好なので好ましく、特に綿１００％のものが望
ましい。The non-woven fabric used in the present invention is preferably one containing a large amount of cotton, since it has good moisturizing properties, touch to the skin, wiping-off properties, etc., and is particularly preferably 100% cotton.
【０００５】＜試験例１＞ （１）目 的 一定の大きさの木綿不織布に消毒薬を含浸させ、１０秒
間及び３０秒間菌液を作用させ、その殺菌効果を判定す
る。 （２）試験薬剤 （イ）塩化ベンザルコニウム主材の試験消毒液 塩化ベンザルコニウム（１０ｗ／ｖ）（不二薬工、以下
ＢＡＣと略す）を原液として蒸留水で、塩化ベンザルコ
ニウムの１００倍、１０００倍、及び１００００倍液を
調整する。これを１００倍、１０００倍、及び１０００
０倍希釈ＢＡＣとする。また、前記希釈液の各々に、エ
タノ−ル（和光純薬、９９．５％試験特級）を最終濃度
が１０％及び３０％になるように添加し、これを塩化ベ
ンザルコニウム主剤の試験消毒液とする。 （ロ）アルコ−ル消毒液 蒸留水にエタノ−ルを最終濃度が１０％及び３０％にな
るように添加し、これをアルコ−ル消毒液とする。 （３）試験菌 （イ）Staphylococcus aureus IFO 12732(FDA 209P) （ロ）Escherichin coli. IFO 3301 （４）試験方法 １５ｃｍ×７．５ｃｍの木綿製不織布を４つ折りにし、
これに試験薬剤を２ｍｌずつ含浸させる（対照として蒸
留水２ｍｌを含浸させる。）。この薬剤含浸布に試験菌
の懸濁液（１０7 個／ｍｌ）の０．１ｍｌを布で包み込
むようにして浸み込ませ、１０秒間、及び３０秒間もみ
ながら接触作用させる。 作用終了直後にストマッカ−用
袋に取り、これに１００ｍｌの生理食塩液で段階希釈
し、各希釈液１ｍｌをトリブチケ−スソイ寒天培地に混
釈培養する。３７°Ｃで２４時間培養後、生残した菌数
を数え、消毒効果を見る。 （５）試験結果 試験結果を、次の表１及び表２に示す。<Test Example 1> (1) Purpose A cotton nonwoven fabric of a certain size is impregnated with a disinfectant, and a bacterial solution is allowed to act for 10 seconds and 30 seconds to determine the sterilizing effect. (2) Test agent (a) Test disinfectant solution of benzalkonium chloride main material Benzalkonium chloride (10 w / v) (Fujiyakuko, hereinafter abbreviated as BAC) was used as a stock solution with distilled water to remove benzalkonium chloride. Prepare 100x, 1000x, and 10000x solutions. This is 100 times, 1000 times, and 1000 times
Use 0-fold diluted BAC. Also, ethanol (Wako Pure Chemical Industries, 99.5% test grade) was added to each of the above diluting solutions so that the final concentrations were 10% and 30%, and this was tested and disinfected with benzalkonium chloride main agent. Use as liquid. (B) Alcohol disinfecting solution Ethanol is added to distilled water to a final concentration of 10% and 30%, and this is used as an alcohol disinfecting solution. (3) Test bacterium (a) Staphylococcus aureus IFO 12732 (FDA 209P) (b) Escherichin coli. IFO 3301 (4) Test method Fold a cotton non-woven fabric of 15 cm x 7.5 cm into four,
This is impregnated with 2 ml each of the test agent (2 ml of distilled water is impregnated as a control). A 0.1 ml of a suspension (10 7 cells / ml) of the test bacterium is wrapped in the cloth soaked in the drug-impregnated cloth, and the cloth is impregnated with the cloth so that the cloth may be contacted for 10 seconds and 30 seconds. Immediately after the end of the action, it is taken in a bag for a stomacher, serially diluted with 100 ml of a physiological saline solution, and 1 ml of each diluted solution is pour-cultured in a Tributykes-Soy agar medium. After culturing at 37 ° C for 24 hours, the number of surviving bacteria is counted and the disinfection effect is observed. (5) Test results The test results are shown in Tables 1 and 2 below.
【０００６】[0006]
【表１】 [Table 1]
【０００７】[0007]
【表２】 [Table 2]
【０００８】表１及び表２の結果は、ＢＡＣとエタノー
ルの混合水溶液を使用すれば、いわゆる相乗的な殺菌効
果が得られることを示している。The results in Tables 1 and 2 show that a so-called synergistic bactericidal effect can be obtained by using a mixed aqueous solution of BAC and ethanol.
【０００９】＜試験例２＞ （１）目 的 一定の大きさの３種のふき取り基材（ガーゼ、脱脂綿、
及び不織布）に消毒液を含浸させ、ふき取り法により手
指の殺菌効果を見る。 （２）ふき取り基材 （Ａ）ガーゼ （Ｂ）脱脂綿 （Ｃ）不織布 （３）薬剤 （イ）ＢＡＣ１００倍希釈液＋１０％エタノール溶液 （ロ）ＢＡＣ１００倍希釈液＋３０％エタノール溶液 （４）実験方法 各基材（１５×１５ｃｍ）に（イ）または（ロ）の薬剤
を一定量（４．０ｍｌ）含浸させ、 それで手指をふき取
った後、 手指に付着している全菌数を測定した。なお、
培地は普通寒天培地（栄研・Ｋ・Ｋ) を用いた。 （５）試験結果 試験結果を、次の表３に示す。<Test Example 2> (1) Objective Three types of wiping base materials (gauze, absorbent cotton,
And non-woven fabric) is impregnated with a disinfectant solution, and the sterilizing effect of fingers is observed by the wiping method. (2) Wiping base material (A) Gauze (B) Cotton wool (C) Nonwoven fabric (3) Chemicals (A) BAC 100-fold diluted solution + 10% ethanol solution (B) BAC 100-fold diluted solution + 30% ethanol solution (4) Experimental method The base material (15 × 15 cm) was impregnated with a certain amount (4.0 ml) of the drug (a) or (b), and the fingers were wiped off, and the total number of bacteria adhering to the fingers was measured. In addition,
A common agar medium (Eiken / K / K) was used as the medium. (5) Test results The test results are shown in Table 3 below.
【００１０】[0010]
【表３】 [Table 3]
【００１１】表３の結果は、殺菌消毒液を含浸させる基
材として不織布を使用すれば、ガーゼ、脱脂綿等に比較
して、ふき取り殺菌効果を増強することができることを
示している。The results in Table 3 show that if a non-woven fabric is used as a base material impregnated with a sterilizing / disinfecting solution, the wipe sterilizing effect can be enhanced as compared with gauze and absorbent cotton.
【００１２】＜試験例３＞ （１）目 的 一定の大きさの木綿不織布に消毒薬を含浸させ、３０秒
間、及び２分間菌液を作用させた場合の殺菌効果を判定
する。 （２）試験薬剤（塩化ベンザルコニウム主材の試験消毒
液） 塩化ベンザルコニウム（１０ｗ／ｖ）（不二薬工、以下
ＢＡＣと略す）を原液として蒸留水で、５０倍、１００
倍液を調整する。この各々にエタノ−ル（和光純薬９
９．５％試験特級）を最終濃度が１０％及び３０％にな
るように添加し、これを塩化ベンザルコニウム塩化ベン
ザルコニウム主材の試験消毒液とする。 （３）試験菌 （イ）Staphylococcus aureus IFO 12732(FDA 209P) （ロ）ＭＲＳＡ（メチシリン耐性Ｓtaphylococcus aure
us 分離株） （ハ）Escherichin coli. IFO 3301 （ニ）Ｐseudomonas aeruginosa IFO 3445 （４）試験方法 １５ｃｍ×７．５ｃｍの木綿製不織布を４つ折りにし、
これに試験薬剤を２ｍｌずつ含浸させる（対照として蒸
留水２ｍｌを含浸させる。）。この薬剤含浸布を製品パ
ックに詰め、一週間室温に放置後、試験菌の懸濁液（１
０7 個／ｍｌ）の０．１ｍｌを布で包み込むようにして
浸み込ませ、３０秒間及び２分間、揉みながら接触作用
させる。 作用終了直後にストマッカ−用袋に取り、これ
に１００ｍｌの生理食塩液を加えて３０秒間ストマッカ
−で処理する。この液を生理食塩液で段階希釈し、各希
釈液１ｍｌをトリブチケ−スソイ寒天培地に混釈培養す
る。３７°Ｃで２４時間培養後、菌数を数え、蒸留水を
含浸させた対照と比較する。 （５）試験結果 試験結果を表４及び表５に示す。<Test Example 3> (1) Purpose A cotton nonwoven fabric of a certain size is impregnated with a disinfectant, and the bactericidal effect is evaluated when the bacterial solution is allowed to act for 30 seconds and 2 minutes. (2) Test agent (test disinfectant solution of benzalkonium chloride main material) Benzalkonium chloride (10 w / v) (Fujiyakuko, hereinafter abbreviated as BAC) is used as a stock solution with distilled water, 50 times, 100 times
Prepare the double solution. Ethanol (Wako Pure Chemicals 9
9.5% special grade) is added so that the final concentration is 10% and 30%, and this is used as a test disinfectant for benzalkonium chloride benzalkonium chloride main material. (3) Test bacterium (a) Staphylococcus aureus IFO 12732 (FDA 209P) (b) MRSA (methicillin-resistant Staphylococcus aure)
us Isolate) (C) Escherichin coli. IFO 3301 (D) Pseudomonas aeruginosa IFO 3445 (4) Test method Fold a 15 cm x 7.5 cm cotton non-woven fabric into four,
This is impregnated with 2 ml each of the test agent (2 ml of distilled water is impregnated as a control). This chemical-impregnated cloth was packed in a product pack and allowed to stand at room temperature for 1 week.
0.1 ml of (0 7 pieces / ml) is wrapped in a cloth so that it is soaked, and contact is performed for 30 seconds and 2 minutes while rubbing. Immediately after the end of the action, it is taken in a bag for a stomacher, 100 ml of physiological saline is added thereto, and the bag is treated with a stomacher for 30 seconds. This solution is serially diluted with a physiological saline solution, and 1 ml of each diluted solution is pour-cultured on a Tributykes-Sussoy agar medium. After culturing at 37 ° C for 24 hours, the number of bacteria is counted and compared with the control impregnated with distilled water. (5) Test results Test results are shown in Tables 4 and 5.
【００１３】[0013]
【表４】 [Table 4]
【００１４】[0014]
【表５】 [Table 5]
【００１５】表４及び表５の結果は、ＢＡＣとエタノー
ルの混合水溶液を使用すれば、グラム陰性菌、グラム陽
性菌を問わず、いわゆる相乗的な殺菌効果が得られるこ
とを示している。The results in Tables 4 and 5 show that if a mixed aqueous solution of BAC and ethanol is used, a so-called synergistic bactericidal effect can be obtained regardless of whether Gram-negative bacteria or Gram-positive bacteria.
【００１６】[0016]
【発明の効果】この発明は以上のように構成したから、
従来単独では効果の少なかった塩化ベンザルコニウム
（ＢＡＣ）とエタノールを効果的に使用することができ
ると共に、殺菌消毒液を含浸させる基材として不織布を
使用すれば、殺菌消毒後の皮膚の表面に繊維が残ること
もなく、手、指等を効果的に殺菌消毒することができ、
医療・臨床分野に貢献するところが極めて大きい。Since the present invention is constructed as described above,
It is possible to effectively use benzalkonium chloride (BAC) and ethanol, which have been less effective in the past alone, and to use a non-woven fabric as a base material for impregnating a sterilizing and disinfecting solution, the surface of the skin after sterilizing and disinfecting Without leaving fibers, you can effectively sterilize hands, fingers, etc.,
The contribution to the medical and clinical fields is extremely large.
【手続補正書】[Procedure amendment]
【提出日】平成４年１２月１０日[Submission date] December 10, 1992
【手続補正１】[Procedure Amendment 1]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】０００５[Correction target item name] 0005
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【０００５】＜試験例１＞ （１）目 的 一定の大きさの木綿不織布に消毒薬を含浸させ、１０秒
間及び３０秒間菌液を作用させ、その殺菌効果を判定す
る。 （２）試験薬剤 （イ）塩化ベンザルコニウム主材の試験消毒液 塩化ベンザルコニウム（１０ｗ／ｖ）（不二薬工、以下
ＢＡＣと略す）を原液として蒸留水で、塩化ベンザルコ
ニウムの１００倍、１０００倍、及び１００００倍液を
調整する。これを１００倍、１０００倍、及び１０００
０倍希釈ＢＡＣとする。また、前記希釈液の各々に、エ
タノール（和光純薬、９９．５％試験特級）を最終濃度
が１０％及び３０％になるように添加し、これを塩化ベ
ンザルコニウム主剤の試験消毒液とする。 （ロ）アルコール消毒液 蒸留水にエタノールを最終濃度が１０％及び３０％にな
るように添加し、これをアルコール消毒液とする。 （３）試験菌 （イ）Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓ
ＩＦＯ １２７３２（ＦＤＡ ２０９Ｐ） （ロ）Ｅｓｃｈｅｒｉｃｈｉａ ｃｏｌｉ．ＩＦＯ ３
３０１ （４）試験方法 １５ｃｍ×７．５ｃｍの木綿製不織布を４つ折りにし、
これに試験薬剤を２ｍｌずつ含浸させる（対照として蒸
留水２ｍｌを含浸させる。）。この薬剤含浸布に試験菌
の懸濁液（１０７個／ｍｌ）の０．１ｍｌを布で包み込
むようにして浸み込ませ、１０秒間、及び３０秒間もみ
ながら接触作用させる。作用終了直後にストマッカー用
袋に取り、これに１００ｍｌの生理食塩液で段階希釈
し、各希釈液１ｍｌをトリブチケースソイ寒天培地に混
釈培養する。３７°Ｃで２４時間培養後、生残した菌数
を数え、消毒効果を見る。 （５）試験結果 試験結果を、次の表１及び表２に示す。<Test Example 1> (1) Purpose A cotton nonwoven fabric of a certain size is impregnated with a disinfectant, and a bacterial solution is allowed to act for 10 seconds and 30 seconds to determine the sterilizing effect. (2) Test agent (a) Test disinfectant solution of benzalkonium chloride main material Benzalkonium chloride (10 w / v) (Fujiyakuko, hereinafter abbreviated as BAC) was used as a stock solution with distilled water to remove benzalkonium chloride. Prepare 100x, 1000x, and 10000x solutions. This is 100 times, 1000 times, and 1000 times
Use 0-fold diluted BAC. In addition, ethanol (Wako Pure Chemical Industries, 99.5% test grade) was added to each of the above diluting solutions so that the final concentrations were 10% and 30%, and this was used as a test disinfecting solution for the benzalkonium chloride base agent. To do. (B) Alcohol disinfectant solution Ethanol is added to distilled water to a final concentration of 10% and 30%, and this is used as an alcohol disinfectant solution. (3) Test bacterium (a) Staphylococcus aureus
IFO 12732 (FDA 209P) (b) Escherichi a coli. IFO 3
301 (4) Test method Fold a cotton non-woven fabric of 15 cm × 7.5 cm into four,
This is impregnated with 2 ml each of the test agent (2 ml of distilled water is impregnated as a control). A 0.1 ml of a suspension (10 7 cells / ml) of the test bacteria is wrapped in the cloth impregnated with the chemical and impregnated with the cloth, and the cloth is impregnated with the cloth for 10 seconds and 30 seconds for contact. Immediately after the end of the action, it is taken in a bag for a stomacher, serially diluted with 100 ml of a physiological saline solution, and 1 ml of each diluted solution is pour-cultured on Tributycase soy agar medium. After culturing at 37 ° C for 24 hours, the number of surviving bacteria is counted and the disinfection effect is observed. (5) Test results The test results are shown in Tables 1 and 2 below.
【手続補正２】[Procedure Amendment 2]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】０００９[Correction target item name] 0009
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【０００９】＜試験例２＞ （１）目 的 一定の大きさの３種のふき取り基材（ガーゼ、脱脂綿、
及び不織布）に消毒液を含浸させ、ふき取り法により手
指の殺菌効果を見る。 （２）ふき取り基材 （Ａ）ガーゼ （Ｂ）脱脂綿 （Ｃ）不織布 （３）薬剤 （イ）ＢＡＣ１００倍希釈液＋１０％エタノール溶液 （ロ）ＢＡＣ１００倍希釈液＋３０％エタノール溶液 （４）実験方法 各基材（１５×１５ｃｍ）に（イ）または（ロ）の薬剤
を一定量（４．０ｍｌ）含浸させ、それで手指をふき取
った後、手指に付着している全菌数を測定した。なお、
培地は普通寒天培地（栄研・Ｋ・Ｋ）を用いた。 （５）試験結果 試験結果を、次の表３に示す。<Test Example 2> (1) Objective Three types of wiping base materials (gauze, absorbent cotton,
And non-woven fabric) is impregnated with a disinfectant solution, and the sterilizing effect of fingers is observed by the wiping method. (2) wiping the substrate (A) gauze (B) cotton (C) non-woven fabric (3) a drug (b) BAC100 dilution + 10% ethanol solution (b) BAC100 dilution + 30% ethanol solution (4) Experimental method Each A base material (15 × 15 cm) was impregnated with a certain amount (4.0 ml) of the drug ( a ) or ( b ), and the fingers were wiped off, and the total number of bacteria adhering to the fingers was measured. In addition,
The medium used was an ordinary agar medium (Eiken / K / K). (5) Test results The test results are shown in Table 3 below.
【手続補正３】[Procedure 3]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】００１２[Correction target item name] 0012
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【００１２】＜試験例３＞ （１）目 的 一定の大きさの木綿不織布に消毒薬を含浸させ、３０秒
間、及び２分間菌液を作用させた場合の殺菌効果を判定
する。 （２）試験薬剤（塩化ベンザルコニウム主材の試験消毒
液） 塩化ベンザルコニウム（１０ｗ／ｖ）（不二薬工、以下
ＢＡＣと略す）を原液として蒸留水で、５０倍、１００
倍液を調整する。この各々にエタノール（和光純薬９
９．５％試験特級）を最終濃度が１０％及び３０％にな
るように添加し、これを塩化ベンザルコニウム塩化ベン
ザルコニウム主材の試験消毒液とする。 （３）試験菌 （イ）Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓ
ＩＦＯ １２７３２（ＦＤＡ ２０９Ｐ） （ロ）ＭＲＳＡ（メチシリン耐性Ｓ ｔａｐｈｙｌｏｃ
ｏｃｃｕｓ ａｕｒｅｕｓ 分離株） （ハ）Ｅｓｃｈｅｒｉｃｈｉａ ｃｏｌｉ．ＩＦＯ ３
３０１ （ニ）Ｐ ｓｅｕｄｏｍｏｎａｓ ａｅｒｕｇｉｎｏｓ
ａ ＩＦＯ ３４４５ （４）試験方法 １５ｃｍ×７．５ｃｍの木綿製不織布を４つ折りにし、
これに試験薬剤を２ｍｌずつ含浸させる（対照として蒸
留水２ｍｌを含浸させる。）。この薬剤含浸布を製品パ
ックに詰め、一週間室温に放置後、試験菌の懸濁液（１
０７個／ｍｌ）の０．１ｍｌを布で包み込むようにして
浸み込ませ、３０秒間及び２分間、揉みながら接触作用
させる。作用終了直後にストマッカー用袋に取り、これ
に１００ｍｌの生理食塩液を加えて３０秒間ストマッカ
ーで処理する。この液を生理食塩液で段階希釈し、各希
釈液１ｍｌをトリブチケースソイ寒天培地に混釈培養す
る。３７°Ｃで２４時間培養後、菌数を数え、蒸留水を
含浸させた対照と比較する。 （５）試験結果 試験結果を表４及び表５に示す。<Test Example 3> (1) Purpose A cotton nonwoven fabric of a certain size is impregnated with a disinfectant, and the bactericidal effect is evaluated when the bacterial solution is allowed to act for 30 seconds and 2 minutes. (2) Test agent (test disinfectant solution of benzalkonium chloride main material) Benzalkonium chloride (10 w / v) (Fujiyakuko, hereinafter abbreviated as BAC) is used as a stock solution with distilled water, 50 times, 100 times
Prepare the double solution. Ethanol (Wako Pure Chemicals 9
9.5% special grade) is added so that the final concentration is 10% and 30%, and this is used as a test disinfectant for benzalkonium chloride benzalkonium chloride main material. (3) Test bacterium (a) Staphylococcus aureus
IFO 12732 (FDA 209P) (b) MRSA (methicillin-resistant S taphyloc
occus aureus isolates) (c) Escherichi a coli. IFO 3
301 (d) P seudomonas aeruginos
a IFO 3445 (4) Test method Fold 15 cm x 7.5 cm cotton nonwoven fabric into four,
This is impregnated with 2 ml each of the test agent (2 ml of distilled water is impregnated as a control). This chemical-impregnated cloth was packed in a product pack and allowed to stand at room temperature for 1 week.
The 0.1ml of 0 7 / ml) impregnated in the enveloping cloth, 30 seconds and 2 minutes, contacting action while rubbing. Immediately after the end of the action, it is taken up in a bag for a stomacher, 100 ml of physiological saline is added thereto, and the bag is treated with a stomacher for 30 seconds. This solution is serially diluted with physiological saline, and 1 ml of each diluted solution is pour-cultured on Tributycase soy agar medium. After culturing at 37 ° C for 24 hours, the number of bacteria is counted and compared with the control impregnated with distilled water. (5) Test results Test results are shown in Tables 4 and 5.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.5 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ａ６１Ｌ 15/44 // Ｃ０７Ｃ 211/63 6917−4Ｈ (Ａ６１Ｋ 31/14 31:045） 8413−4Ｃ (72)発明者 福嶋 祥介 神奈川県横浜市中区長者町８丁目133番地 (72)発明者 伊東 ▲たい▼白 神奈川県相模原市共和２丁目５番16号─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 5 Identification code Internal reference number FI Technical display location A61L 15/44 // C07C 211/63 6917-4H (A61K 31/14 31: 045) 8413-4C (72) Inventor Shosuke Fukushima 8-133 Chojamachi, Naka-ku, Yokohama-shi, Kanagawa Prefecture (72) Inventor Ito ▲ tai ▼ Shirohara, Kanagawa Prefecture 2-5-16 Kyowa, Sagamihara-shi
Claims (5)
ノール、及び水の三者を含有することを特徴とする殺菌
消毒液。1. A sterilizing solution containing benzalkonium chloride (BAC), ethanol and water.
ノール、及び水の三者の比が、１〜５０：５０〜３００
０：５００〜１００００である請求項１に記載する殺菌
消毒液。2. The ratio of benzalkonium chloride (BAC), ethanol, and water is 1 to 50:50 to 300.
The sterilizing and disinfecting solution according to claim 1, which is 0: 500 to 10,000.
ノール、及び水の三者を含有する殺菌消毒液を不織布に
含浸させたことを特徴とする殺菌消毒材。3. A sterilizing and disinfecting material, wherein a nonwoven fabric is impregnated with a sterilizing and disinfecting solution containing benzalkonium chloride (BAC), ethanol and water.
に記載する殺菌消毒材。4. The non-woven fabric material is mainly cotton.
The sterilizing and disinfecting material described in.
ノール、及び水の三者の比が、１〜５０：５０〜３００
０：５００〜１００００である殺菌消毒液を使用する請
求項３及び請求項４に記載する殺菌消毒材。5. The ratio of benzalkonium chloride (BAC), ethanol, and water is from 1 to 50:50 to 300.
The sterilizing material according to claim 3 or 4, which uses a sterilizing solution of 0: 500 to 10,000.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3321575A JPH05124910A (en) | 1991-10-31 | 1991-10-31 | Sterilizing and disinfecting liquid and sterilizing and disinfecting material containing the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3321575A JPH05124910A (en) | 1991-10-31 | 1991-10-31 | Sterilizing and disinfecting liquid and sterilizing and disinfecting material containing the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH05124910A true JPH05124910A (en) | 1993-05-21 |
Family
ID=18134094
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3321575A Pending JPH05124910A (en) | 1991-10-31 | 1991-10-31 | Sterilizing and disinfecting liquid and sterilizing and disinfecting material containing the same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH05124910A (en) |
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|---|---|---|---|---|
| JP2000273004A (en) * | 1999-03-19 | 2000-10-03 | Nicca Chemical Co Ltd | Germicidal disinfectant composition |
| JP2002193706A (en) * | 2000-12-25 | 2002-07-10 | Kenei Pharmaceutical Co Ltd | Antibacterial disinfectant for external use |
| KR100425943B1 (en) * | 2000-11-30 | 2004-04-03 | 구본길 | Germicide with the skin care of lotion type |
| WO2005079872A1 (en) * | 2004-02-23 | 2005-09-01 | Kobayashi Pharmaceutical Co., Ltd. | Composition for air sterilization and method of air sterilization |
| WO2005079873A1 (en) * | 2004-02-23 | 2005-09-01 | Kobayashi Pharmaceutical Co., Ltd. | Composition for air sterilization and method of air sterilization |
| US7541045B2 (en) | 1996-04-12 | 2009-06-02 | University Of Arkansas | Concentrated, non-foaming solution of quaternary ammonium compounds and methods of use |
| WO2010061764A1 (en) * | 2008-11-27 | 2010-06-03 | ユニ・チャーム株式会社 | Chemical liquid for alcohol sterilization sheet and alcohol sterilization sheet containing same |
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-
1991
- 1991-10-31 JP JP3321575A patent/JPH05124910A/en active Pending
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7541045B2 (en) | 1996-04-12 | 2009-06-02 | University Of Arkansas | Concentrated, non-foaming solution of quaternary ammonium compounds and methods of use |
| JP2000273004A (en) * | 1999-03-19 | 2000-10-03 | Nicca Chemical Co Ltd | Germicidal disinfectant composition |
| US8771731B2 (en) | 1999-04-28 | 2014-07-08 | The Regents Of The University Of Michigan | Antimicrobial nanoemulsion compositions and methods |
| JP2012136533A (en) * | 1999-12-30 | 2012-07-19 | Regents Of The Univ Of Michigan | Antimicrobial composition and method for using the same |
| KR100425943B1 (en) * | 2000-11-30 | 2004-04-03 | 구본길 | Germicide with the skin care of lotion type |
| JP2002193706A (en) * | 2000-12-25 | 2002-07-10 | Kenei Pharmaceutical Co Ltd | Antibacterial disinfectant for external use |
| WO2005079873A1 (en) * | 2004-02-23 | 2005-09-01 | Kobayashi Pharmaceutical Co., Ltd. | Composition for air sterilization and method of air sterilization |
| JP2005230394A (en) * | 2004-02-23 | 2005-09-02 | Kobayashi Pharmaceut Co Ltd | Composition for space sterilization and space sterilization method |
| JP4632676B2 (en) * | 2004-02-23 | 2011-02-16 | 小林製薬株式会社 | Spatial disinfection composition and spatial disinfection method |
| JP4632675B2 (en) * | 2004-02-23 | 2011-02-16 | 小林製薬株式会社 | Spatial disinfection composition and spatial disinfection method |
| JP2005230395A (en) * | 2004-02-23 | 2005-09-02 | Kobayashi Pharmaceut Co Ltd | Composition for space sterilization and space sterilization method |
| WO2005079872A1 (en) * | 2004-02-23 | 2005-09-01 | Kobayashi Pharmaceutical Co., Ltd. | Composition for air sterilization and method of air sterilization |
| US9801842B2 (en) | 2007-05-02 | 2017-10-31 | The Regents Of The University Of Michigan | Nanoemulsion therapeutic compositions and methods of using the same |
| US9974844B2 (en) | 2008-11-17 | 2018-05-22 | The Regents Of The University Of Michigan | Cancer vaccine compositions and methods of using the same |
| US11116825B2 (en) | 2008-11-17 | 2021-09-14 | The Regents Of The University Of Michigan | Cancer vaccine compositions and methods of using the same |
| WO2010061764A1 (en) * | 2008-11-27 | 2010-06-03 | ユニ・チャーム株式会社 | Chemical liquid for alcohol sterilization sheet and alcohol sterilization sheet containing same |
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