JPH0499448A - Oil seed-derived protein - Google Patents
Oil seed-derived proteinInfo
- Publication number
- JPH0499448A JPH0499448A JP2214749A JP21474990A JPH0499448A JP H0499448 A JPH0499448 A JP H0499448A JP 2214749 A JP2214749 A JP 2214749A JP 21474990 A JP21474990 A JP 21474990A JP H0499448 A JPH0499448 A JP H0499448A
- Authority
- JP
- Japan
- Prior art keywords
- oilseed
- derived protein
- protein
- oil seed
- molecular weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 41
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 41
- 238000004062 sedimentation Methods 0.000 claims abstract description 10
- 244000068988 Glycine max Species 0.000 claims abstract description 9
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 9
- 230000002255 enzymatic effect Effects 0.000 claims abstract description 8
- 239000000284 extract Substances 0.000 claims abstract description 8
- 239000012528 membrane Substances 0.000 claims abstract description 6
- 239000007864 aqueous solution Substances 0.000 claims abstract description 5
- 229910052751 metal Inorganic materials 0.000 claims abstract description 3
- 239000002184 metal Substances 0.000 claims abstract description 3
- 150000003839 salts Chemical class 0.000 claims abstract description 3
- 238000000108 ultra-filtration Methods 0.000 claims description 7
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical group CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 claims description 2
- 244000105624 Arachis hypogaea Species 0.000 claims description 2
- 244000056139 Brassica cretica Species 0.000 claims description 2
- 235000003351 Brassica cretica Nutrition 0.000 claims description 2
- 240000002791 Brassica napus Species 0.000 claims description 2
- 235000003343 Brassica rupestris Nutrition 0.000 claims description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 2
- 244000020518 Carthamus tinctorius Species 0.000 claims description 2
- 235000003255 Carthamus tinctorius Nutrition 0.000 claims description 2
- 235000003222 Helianthus annuus Nutrition 0.000 claims description 2
- 102000015439 Phospholipases Human genes 0.000 claims description 2
- 108010064785 Phospholipases Proteins 0.000 claims description 2
- 244000000231 Sesamum indicum Species 0.000 claims description 2
- 235000003434 Sesamum indicum Nutrition 0.000 claims description 2
- 240000008042 Zea mays Species 0.000 claims description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 2
- QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 claims description 2
- 235000005822 corn Nutrition 0.000 claims description 2
- 235000012343 cottonseed oil Nutrition 0.000 claims description 2
- 235000010460 mustard Nutrition 0.000 claims description 2
- 235000020232 peanut Nutrition 0.000 claims description 2
- 235000017060 Arachis glabrata Nutrition 0.000 claims 1
- 235000010777 Arachis hypogaea Nutrition 0.000 claims 1
- 235000018262 Arachis monticola Nutrition 0.000 claims 1
- 244000020551 Helianthus annuus Species 0.000 claims 1
- 240000007817 Olea europaea Species 0.000 claims 1
- 235000013305 food Nutrition 0.000 abstract description 6
- 239000000796 flavoring agent Substances 0.000 abstract description 4
- 235000019634 flavors Nutrition 0.000 abstract description 3
- 239000004615 ingredient Substances 0.000 abstract description 2
- 150000003904 phospholipids Chemical class 0.000 abstract description 2
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 28
- 230000000694 effects Effects 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 239000000463 material Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 102000003820 Lipoxygenases Human genes 0.000 description 2
- 108090000128 Lipoxygenases Proteins 0.000 description 2
- 102000011420 Phospholipase D Human genes 0.000 description 2
- 108090000553 Phospholipase D Proteins 0.000 description 2
- 102000014384 Type C Phospholipases Human genes 0.000 description 2
- 108010079194 Type C Phospholipases Proteins 0.000 description 2
- 108010019077 beta-Amylase Proteins 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 206010000060 Abdominal distension Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000208818 Helianthus Species 0.000 description 1
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
- 241000207836 Olea <angiosperm> Species 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 108010073771 Soybean Proteins Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 208000024330 bloating Diseases 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 235000019645 odor Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229940068041 phytic acid Drugs 0.000 description 1
- 235000002949 phytic acid Nutrition 0.000 description 1
- 239000000467 phytic acid Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000019710 soybean protein Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は油糧種子由来蛋白質に関し、詳しくは油糧種子
から得た沈降定数が２Ｓ〜７Ｓである蛋白質であって、
かつ酵素活性を有する油糧種子由来蛋白質に関する。[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to an oilseed-derived protein, and more specifically, a protein obtained from an oilseed and having a sedimentation constant of 2S to 7S,
The present invention also relates to an oilseed-derived protein having enzymatic activity.
〔従来の技術及び本発明が解決しようとする課題〕従来
、油糧種子から蛋白質は、その製造過程において、より
多くの蛋白質を溶出させる条件で抽出がなされ、全ての
範囲にわたる分子量画分の物質が抽出されてきた。[Prior art and the problem to be solved by the present invention] Conventionally, proteins from oilseeds have been extracted under conditions that elute a larger amount of protein during the production process, and substances in molecular weight fractions covering a whole range are extracted. has been extracted.
油糧種子由来蛋白質のうち特に大豆において酸やアルカ
リの処理で得られる分子量範囲が約６万〜２０万のいわ
ゆる７Ｓ蛋白質はそのカルシウムゲルまたは加熱ゲルが
、極めて保水性が高く柔らかい物性であるため、食品加
工用素材として極めて有用であることが知られている。Among oilseed-derived proteins, the so-called 7S protein, which has a molecular weight range of about 60,000 to 200,000 and can be obtained from soybeans in particular by acid or alkali treatment, has extremely high water retention and soft physical properties in its calcium gel or heated gel. , is known to be extremely useful as a food processing material.
また油糧種子には様々な酵素が含まれており、これらは
例えば大豆においてはβ−アミラーゼが分子量約６万、
リポキシゲナーゼ１，２．３ａ。Oilseeds also contain various enzymes, such as β-amylase in soybeans, which has a molecular weight of approximately 60,000;
Lipoxygenase 1,2.3a.
３ｂがそれぞれ約１０万等に示されるように、さほど分
子量が高くないところに酵素が集中している。As shown in the figure of approximately 100,000 yen each for 3b, the enzymes are concentrated in areas where the molecular weight is not very high.
これに対し、油糧種子抽出物のうち沈降定数で１１３以
上の高分子画分蛋白質は機能性も低く、酵素も余り存在
しない。On the other hand, among oilseed extracts, high molecular fraction proteins with a sedimentation constant of 113 or more have low functionality and do not contain many enzymes.
また低分子画分は、低分子゛蛋白質、及びペプチドのほ
か、青臭み、刺激臭、酸味、バターワックス臭等の、油
糧種子由来の異臭成分であり、この異臭成分のため油糧
種子由来蛋白質の利用範囲が狭められていた。さらにこ
の画分には、ミネラルに対してキレートを形成し、体内
への吸収を阻害するフィチン酸、又、腸内鼓張因子であ
るオリゴ糖等が含まれており、栄養学的に好ましくない
成分が含まれていた。In addition to low molecular weight proteins and peptides, the low-molecular fraction contains off-flavor components derived from oilseeds such as grassy, pungent, sour, and butterwax odors. The scope of protein utilization has been narrowed. Furthermore, this fraction contains phytic acid, which forms a chelate with minerals and inhibits their absorption into the body, and oligosaccharides, which are factors that cause intestinal bloating, which are nutritionally undesirable. contained the ingredients.
このように油糧種子由来の蛋白質に機能性を求めるとき
、又は酵素を分画しようとすると高分子画分蛋白質、及
び低分子画分はあまり関与しない夾雑物質となる。In this way, when seeking functionality from proteins derived from oilseeds or attempting to fractionate enzymes, high molecular fraction proteins and low molecular fractions become contaminants that do not participate much.
そして、高分子画分蛋白質並びに低分子画分蛋白質を除
いた油糧種子蛋白質については、用途或いは機能に関し
て詳細な検討がなされていないのが現状である。At present, no detailed studies have been conducted regarding the uses or functions of oilseed proteins other than high molecular fraction proteins and low molecular fraction proteins.
かかる現状において、本発明者らは上記課題を解決すべ
く鋭意研究の結果、本発明を完成するに至った。Under such current circumstances, the present inventors have completed the present invention as a result of intensive research to solve the above problems.
即ち、本発明は、沈降定数が２Ｓ〜７Ｓの範囲にある油
糧種子由来蛋白質を提供するものである。That is, the present invention provides an oilseed-derived protein having a sedimentation constant in the range of 2S to 7S.
本発明で沈降定数が２Ｓ〜７Ｓである分子量範囲を有す
る蛋白質とは分子量が１万〜２０万である蛋白質を示す
ものであり、好ましくは分子量１万〜１０万である蛋白
質を示すものである。In the present invention, a protein having a molecular weight range with a sedimentation constant of 2S to 7S refers to a protein with a molecular weight of 10,000 to 200,000, preferably a protein with a molecular weight of 10,000 to 100,000. .
本発明の特定の分子量範囲を有する油糧種子蛋白質はま
ず、油糧種子をそのままか、あるいは粉砕した物に、濃
度が０．０１ ｍＭ〜１Ｍの範囲で、９Ｈが５．０〜７
．０の範囲の無機及び／又は有機金属塩類水溶液を加え
、０〜４０°Ｃの範囲の温度にて蛋白質を抽出し、その
後、該抽出液を限外濾過膜により処理することにより得
られる。The oilseed protein having a specific molecular weight range of the present invention is prepared by adding 9H of 5.0 to 7 to the oilseed as it is or to the pulverized product at a concentration of 0.01 mM to 1M.
．． It is obtained by adding an aqueous solution of inorganic and/or organic metal salts in the range of 0°C to extract proteins at a temperature in the range of 0 to 40°C, and then treating the extract with an ultrafiltration membrane.
本発明において、限外濾過に用いる限外濾過膜の分画分
子量は少なくとも５０００であることが好ましく、更に
好ましくは１００００以上である。In the present invention, the ultrafiltration membrane used for ultrafiltration preferably has a molecular weight cut-off of at least 5,000, more preferably 10,000 or more.
本発明に好適な分画分子量の蛋白質を得るためにはさら
に分画分子量がｌＯ万以下である限外濾過膜を用いた限
外濾過を行うのが好ましい。In order to obtain a protein with a molecular weight cut-off suitable for the present invention, it is preferable to further perform ultrafiltration using an ultrafiltration membrane having a molecular weight cut-off of 100,000 or less.
本発明に用いられる油糧種子は、大豆、なたね、ひまわ
り、落花生、綿実、紅花、辛子、胡麻、オリーブ、コー
ン等が挙げられ、経済性、蛋白含有量、酵素活性の点か
ら、大豆が好ましい。Oilseeds used in the present invention include soybeans, rapeseed, sunflowers, peanuts, cottonseeds, safflowers, mustard, sesame, olives, and corn. is preferred.
油糧種子はそのままか、あるいは粉砕してもよく、その
場合粉砕物の平均粒径が０．１ｍｍ以上であることが望
ましい。The oilseeds may be used as they are or may be pulverized, and in that case it is desirable that the average particle size of the pulverized product is 0.1 mm or more.
以上の工程を経て沈降定数が２ｓ〜７Ｓ（分子量範囲が
１万〜２０万、好ましくは１万〜１０万）であって、か
つ酵素活性を有する油糧種子由来の蛋白質が製造される
が、このものは水溶液の状態でも良く、また酵素活性を
保持したままの乾燥法で乾燥させた固体の状態でもよく
、その形態を規定するものではない。Through the above steps, an oilseed-derived protein with a sedimentation constant of 2s to 7S (molecular weight range of 10,000 to 200,000, preferably 10,000 to 100,000) and an enzyme activity is produced. This product may be in the form of an aqueous solution or may be in the form of a solid dried by a drying method while retaining the enzyme activity, and its form is not limited.
本発明で得られる油糧種子由来蛋白質が有する酵素活性
とは、主としてホスホリパーゼに由来し、特に好ましく
はホスホリパーゼＣ、ホスホリパーゼＤに由来するもの
である。The enzyme activity possessed by the oilseed-derived protein obtained in the present invention is mainly derived from phospholipase, and particularly preferably from phospholipase C and phospholipase D.
本発明で得られる特定分子量範囲を持ち、かつ酵素活性
を有する油糧種子由来蛋白質は、その酵素活性を利用し
て、各種リン脂質の改質に利用できる他、風味成分を全
（含まないことから、各種飲料、栄養食品等の食品素材
としても利用できる。The oilseed-derived protein having a specific molecular weight range and enzymatic activity obtained by the present invention can be used to modify various phospholipids by utilizing its enzymatic activity. It can also be used as a food material for various beverages, nutritional foods, etc.
〔実施例］
以下に本発明の実施例を述べるが、本発明はこれらの実
施例に限定されるものではない。[Examples] Examples of the present invention will be described below, but the present invention is not limited to these Examples.
実施例１
大豆を平均粒径２．０−■になるように粉砕し、これに
温度２５℃においてｐＨ６の０．０５Ｍ酢酸ナトリウム
水溶液を大豆に対して６倍重量加え、２時間浸漬した後
、大豆を分離し、沈降定数が１１５以上の高分子画分を
ほとんど含まない大豆蛋白質抽出液を得た。これを分画
分子量１３０００の限外濾過膜で処理し、沈降定数が２
Ｓ〜７Ｓである蛋白質抽出液を得た。この蛋白質抽出液
はβ−アミラーゼ活性、リポキシゲナーゼ活性、ホスホ
リパーゼＤ活性、ホスホリパーゼＣ活性を有していた。Example 1 Soybeans were ground to an average particle size of 2.0-■, and a 0.05M aqueous solution of sodium acetate with a pH of 6 was added to the soybeans at a temperature of 25°C by 6 times the weight of the soybeans, and the mixture was immersed for 2 hours. Soybeans were separated to obtain a soybean protein extract containing almost no polymer fraction with a sedimentation constant of 115 or more. This was treated with an ultrafiltration membrane with a molecular weight cutoff of 13,000, and the sedimentation constant was 2.
Protein extracts ranging from S to 7S were obtained. This protein extract had β-amylase activity, lipoxygenase activity, phospholipase D activity, and phospholipase C activity.
本発明で得られる特定分子量範囲を持ち、かつ酵素活性
を有する油糧種子由来蛋白質は、油糧種子特有の風味成
分を全く含有しないため、酵素反応に利用した場合、或
いは食品素材として利用した場合、いずれの場合も反応
生成物或いは食品の風味を低下させることなく品質の向
上を図ることができる。The oilseed-derived protein having a specific molecular weight range and enzymatic activity obtained by the present invention does not contain any flavor components unique to oilseeds, so when used in enzyme reactions or as a food material. In either case, the quality of the reaction product or food can be improved without deteriorating its flavor.
出願人代理人 古 谷 馨 （外３名）Applicant's agent Kaoru Furutani (3 other people)
Claims (1)
白質。 ２、油糧種子をそのままか、あるいは粉砕した物に、濃
度が０．０１ｍＭ〜１Ｍの範囲で、ｐＨが５．０〜７．
０の範囲の無機及び／又は有機金属塩類水溶液を加え、
０〜４０℃の範囲の温度にて蛋白質を抽出し、その後、
該抽出液を限外濾過膜により処理することにより得られ
る、特定分子量範囲を持ち、なおかつ酵素活性を有する
請求項１記載の油糧種子由来蛋白質。 ３、油糧種子が、大豆、なたね、ひまわり、落花生、綿
実、紅花、辛子、胡麻、オリーブ及びコーンからなる群
から選ばれたものである請求項１記載の油糧種子由来蛋
白質。 ４、油糧種子由来蛋白質が酵素活性を有する請求項１記
載の油糧種子由来蛋白質。 ５、酵素活性を有する油糧種子由来蛋白質がホスホリパ
ーゼである請求項４記載の油糧種子由来蛋白質。[Scope of Claims] 1. An oilseed-derived protein having a sedimentation constant in the range of 2S to 7S. 2. Oilseeds, either as they are or crushed, have a concentration of 0.01mM to 1M and a pH of 5.0 to 7.
Add an aqueous solution of inorganic and/or organic metal salts in the range of 0,
Extract the protein at a temperature ranging from 0 to 40°C, and then
The oilseed-derived protein according to claim 1, which is obtained by treating the extract with an ultrafiltration membrane, has a specific molecular weight range, and has enzymatic activity. 3. The oilseed-derived protein according to claim 1, wherein the oilseed is selected from the group consisting of soybean, rapeseed, sunflower, peanut, cottonseed, safflower, mustard, sesame, olive, and corn. 4. The oilseed-derived protein according to claim 1, wherein the oilseed-derived protein has enzymatic activity. 5. The oilseed-derived protein according to claim 4, wherein the oilseed-derived protein having enzymatic activity is phospholipase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2214749A JPH0499448A (en) | 1990-08-14 | 1990-08-14 | Oil seed-derived protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2214749A JPH0499448A (en) | 1990-08-14 | 1990-08-14 | Oil seed-derived protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0499448A true JPH0499448A (en) | 1992-03-31 |
Family
ID=16660944
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2214749A Pending JPH0499448A (en) | 1990-08-14 | 1990-08-14 | Oil seed-derived protein |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0499448A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5549864A (en) * | 1994-04-14 | 1996-08-27 | Eastman Kodak Company | Process for decurling a strip of photosensitive material |
| JP2007518409A (en) * | 2004-01-20 | 2007-07-12 | バーコン ニュートラサイエンス （エムビー） コーポレイション | Novel canola protein isolate |
| JP2008526240A (en) * | 2005-01-18 | 2008-07-24 | フラウンホーファ−ゲゼルシャフト ツァー フォルデルング デア アンゲバンデン フォルシュンク エー． ファオ． | Method for producing vegetable protein ingredients for ice cream and ice cream containing vegetable protein ingredients |
-
1990
- 1990-08-14 JP JP2214749A patent/JPH0499448A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5549864A (en) * | 1994-04-14 | 1996-08-27 | Eastman Kodak Company | Process for decurling a strip of photosensitive material |
| US5580588A (en) * | 1994-04-14 | 1996-12-03 | Eastman Kodak Company | Apparatus for decurling a strip of photosensitive material |
| JP2007518409A (en) * | 2004-01-20 | 2007-07-12 | バーコン ニュートラサイエンス （エムビー） コーポレイション | Novel canola protein isolate |
| JP2008526240A (en) * | 2005-01-18 | 2008-07-24 | フラウンホーファ−ゲゼルシャフト ツァー フォルデルング デア アンゲバンデン フォルシュンク エー． ファオ． | Method for producing vegetable protein ingredients for ice cream and ice cream containing vegetable protein ingredients |
| JP4824702B2 (en) * | 2005-01-18 | 2011-11-30 | フラウンホーファ−ゲゼルシャフト ツァー フォルデルング デア アンゲバンデン フォルシュンク エー． ファオ． | Method for producing vegetable protein ingredients for ice cream and ice cream containing vegetable protein ingredients |
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