JPH04357953A - Pharmaceutical stuck with antimicrobial material - Google Patents

Abstract

PURPOSE:To provide the pharmaceutical which can release an antimicrobial material consisting of allyl isothiocyanate or an oil-soluble extract contg. the allyl isothiocyanate obtd. from plants contg. the allyl isothiocyanate over a long period of time. CONSTITUTION:This pharmaceutical stuck with the antimicrobial material is prepd. by sticking the antimicrobial material consisting of the allyl isothiocyanate or the oil-soluble extract contg. the allyl isothiocyanate obtd. from a Japanese horse-radish, horse-radish or mustard which is the plant of Brassicaceae to a fibrous base. The releasing effect is exhibited over a long period of time in this way.

Description

[Detailed description of the invention]

0001

[Industrial Application Field] The present invention is used to preserve and maintain quality of processed livestock products, processed marine products, agricultural products, cooked foods, confectionery, feed, etc.
A preparation used for the purpose of protecting drawings from corrosion, treating athlete's foot, removing unpleasant body odor, etc., and attaching substances to the support that inhibit the growth of harmful microorganisms such as bacteria or fungi. It's about what happened.

0002

BACKGROUND OF THE INVENTION If foods are left as they are, microorganisms will easily propagate during storage, resulting in deterioration in quality and spoilage. Furthermore, it has been frequently experienced that microorganisms proliferate in hanging scrolls, books, genealogy trees, etc. during storage, reducing their value. Methods for preventing the growth of these microorganisms include adding preservatives, low-temperature storage, and high-temperature sterilization. Among these, preservatives can be easily used;
Although it does not require any special mechanical equipment, there are doubts about its safety for humans. On the other hand, low-temperature storage and high-temperature sterilization have the disadvantage of requiring time and expensive equipment. In order to solve these problems, allyl isothiocyanate produced by cruciferous plants such as wasabi has attracted attention. Allyl isothiocyanate exhibits a strong antibacterial effect in the form of a gas, but has no harmful effects on the human body. Therefore, attempts have been made to apply it as a preservative for foods. However, allyl isothiocyanate is a liquid at room temperature and is difficult to use as it is. In addition, allyl isothiocyanate is easily vaporized, and even when used to preserve a target object, it evaporates during use, making it difficult to maintain a concentration that provides a preservative effect over a long period of time. Therefore, in addition to molding allyl isothiocyanate and/or extracts from plants containing allyl isothiocyanate into a convenient shape,
The development of a formulation as a preservative with controlled vaporization rate has been awaited. Furthermore, since vaporized allyl isothiocyanate exhibits a strong inhibitory effect on the growth of fungi, it has been surmised that it is also useful for treating athlete's foot and removing body odor resulting from the growth of fungi.

0003

[Problems to be Solved by the Invention] However, until now, there has been no means for continuously releasing gaseous allyl isothiocyanate during the period required for storage and treatment, and this has not been put to practical use. Therefore, the development of antibacterial preparations that suppress the vaporization rate of allyl isothiocyanate has been awaited both in the food field and in the medical field.

0004

[Means for Solving the Problems] As a result of intensive studies in view of the above-mentioned circumstances, the present invention provides allyl isothiocyanate, which is harmless to health and whose gas has strong sterilizing power, to a fibrous support (mat or filter paper). This was done with the focus on the ability to prepare processed antibacterial preparations that improve usability and control the vaporization rate by retaining (including coating and impregnating). That's right.

[0005] The invention of claim 1 is an oil-soluble extract containing allyl isothiocyanate having bactericidal activity or allyl isothiocyanate obtained from wasabi, wasabi radish or mustard, which are plants of the cruciferous family (hereinafter referred to as allyl isothiocyanates). When allyl isothiocyanate is vaporized by making it into an antibacterial agent-adhesive preparation that is attached to a fibrous support under temperature conditions that give it a liquid state, the liquid allyl isothiocyanate is directly applied to the object to be stored. It provides something that suppresses the growth of microorganisms without contact. The invention of claim 2 provides an antibacterial substance adhesion preparation in which the allyl isothiocyanate is dissolved in a fatty acid ester compound or paraffin and then adhered to a fibrous support,
Compared to the case where allyl isothiocyanates are directly attached to a fibrous support, the effect of suppressing the vaporization rate and improving sustained release properties is greater. According to the third aspect of the invention, ethanol is added to the fibrous support together with allyl isothiocyanates for the purpose of eliminating the odor of allyl isothiocyanates. According to the fourth aspect of the present invention, the fibrous support is made of pulp, and thereby mass-produced products can be provided at low cost. The invention according to claim 5 is an antibacterial substance adhesion preparation in which the fatty acid ester compound is a compound selected from glycerin fatty acid ester, sorbitan fatty acid ester, phospholipid, and higher alcohol fatty acid ester.

In a more preferred embodiment of the present invention, allyl isothiocyanates alone or diluted with edible fats and oils or paraffin to 1 to 80% by weight are used in an amount of 0.1 to 15 times the weight of the fibrous support. If necessary, ethanol is added in an amount of 0.5 to 15 times the weight of the fibrous support. Examples of pulp mats constituting the fibrous support include wood pulp, linter pulp, or composites thereof, and examples of filter paper include filter paper for pressure filtration.

[0007]

[Action] Since allyl isothiocyanates are attached to a fibrous support, it can be made into a solid antibacterial preparation, and only prevents liquid allyl isothiocyanates from coming into direct contact with the object to be stored. The required amount of allyl isothiocyanates can be easily generated and handled, improving usability. Furthermore, by dissolving allyl isothiocyanate in a fatty acid ester compound or paraffin and then attaching it to a fibrous support, it is possible to suppress the vaporization rate. In addition, by adding ethanol together with allyl isothiocyanates,
The odor peculiar to allyl isothiocyanate is eliminated, and it can be provided as a highly practical product that does not give off a pungent odor when handled.

[0008]

EXAMPLE In order to confirm the effects of the present invention, the degree of release of allyl isothiocyanate was measured for various samples using the following apparatus. The apparatus is as shown in Figure 1, with 50
A stirring blade 2 made of a magnetic material is provided at the bottom of a 0 ml glass bottle 1, and the atmosphere inside the glass bottle 1 is stirred by a magnetic stirrer 3 on the bottom. A rubber stopper 4 is provided at the top of the glass bottle 1, and a sample mat 6, such as a mat, is suspended inside the bottle 1 with a thread 5. Each experimental device was operated at 0°C and room temperature (20±
The test was conducted under three temperature conditions: 5°C) and 40°C. In the test, the gas in the bottle 7 was sampled with a fine needle.
Allyl isothiocyanate concentration was measured by gas chromatography.

Example 1 Allyl isothiocyanate was diluted with corn oil and
The concentrations were adjusted to % by weight, 10% by weight, and 20% by weight. Separately 500 mg of each of these mixtures was placed on a pulp mat (2.8 mm x 22 mm x 35 mm, weight 83 mg).
A total of nine samples, three of each of the three types of antibacterial substances described above, were prepared. Using these samples (suspending pulp mats one by one)
The degree of release of allyl isothiocyanate in the preparation was measured using the apparatus shown in FIG. The results are shown in Figures 2-4. As is clear from Figures 2 to 4, allyl isothiocyanate gas is released over time even at 0°C for the samples at each concentration, and the amount released is correlated with the concentration of allyl isothiocyanate deposited. It was suggested. At room temperature, significant release was observed for all formulations at any concentration, and the amount of release depended on the concentration of allyl isothiocyanate deposited. At 40°C, an even larger amount of gas was released, but the gas was not released all at once at an early stage, but was released almost linearly until 6 hours after being left.
Sustained release effects were observed even under high temperature conditions.

[0010] Among the above preparations, one 5% by weight allyl isothiocyanate adhering preparation was placed in a polyethylene kitchen bag with a thickness of 0.025 mm along with three small rice cakes (hemispherical with a radius of about 3 cm, about 60 g/piece). and stored at room temperature. As a result, no mold growth was observed even after 14 days of storage, but in a control case in which mochi was stored in a similar bag without adding the mat formulation, green mold appeared on the mochi after 14 days of storage. completely covered the surface. This experiment confirmed the sustainability of the antibacterial effect of this antibacterial substance-attached preparation.

Example 2 100 mg of allyl isothiocyanate was added to a pulp mat (
3 mm x 22 mm x 35 mm) to obtain a sample according to the present invention.

Example 3 100 mg of allyl isothiocyanate was added to 500 m of rapeseed oil.
After dissolving in g, pulp mat (3 mm x 22 mm x
35 mm) to obtain a sample according to the invention.

Example 4 100 mg of allyl isothiocyanate was added to 500 m of rapeseed oil.
g, and further added 500 mg of ethanol. This solution (total 1000 mg) was applied to a pulp mat (3 mm).
x 22 mm x 35 mm) to obtain a sample according to the present invention.

Comparative Example 1 A sample of Comparative Example 1 was prepared by placing 100 mg of allyl isothiocyanate in a glass Petri dish with an inner diameter of 75 mm.

The samples of Examples 2 to 4 and Comparative Example 1 were allowed to stand at room temperature and the released amount (%) of allyl isothiocyanate was examined, as shown in FIG.
Compared to the sample of Comparative Example 1, the release of allyl isothiocyanate was suppressed in Samples No. 4 to 4. In particular, the samples of Examples 3 and 4 showed remarkable sustained release effects. In addition, the sample of Example 4 did not have any aroma peculiar to allyl isothiocyanate. The amount of allyl isothiocyanate released was determined by measuring the weight loss of the pulp mat or petri dish. (The same applies to the following examples.)

[
Example 5 250 mg of an oil-soluble extract containing allyl isothiocyanate extracted from mustard seeds was placed in a pulp mat (3 mm x 44
A sample according to the present invention was obtained by attaching the sample to an area (mm x 70 mm).

Example 6 After dissolving 250 mg of the allyl isothiocyanate-containing oil-soluble extract used in Example 5 in 1750 mg of liquid paraffin, a pulp mat (3 mm x 44 mm x 70 mm) was prepared.
) to obtain a sample according to the present invention.

Example 7 After 250 mg of the allyl isothiocyanate-containing oil-soluble extract used in Example 5 was dissolved in 1750 mg of liquid paraffin, 1600 mg of ethanol was further added. Apply this solution to a pulp mat (3mm x 44mm x 70mm)
A sample according to the present invention was obtained.

Comparative Example 2 A sample of Comparative Example 2 was prepared by placing 225 mg of allyl isothiocyanate in a glass Petri dish with an inner diameter of 75 mm.

The samples of Examples 5 to 7 and Comparative Example 2 were allowed to stand at room temperature and the released amount (%) of allyl isothiocyanate was examined, as shown in FIG.
Compared with the sample of Comparative Example 2, the release of allyl isothiocyanate was suppressed in the samples from 1 to 7. In particular, the samples of Examples 6 and 7 were found to have a remarkable sustained release effect. In addition, the sample of Example 7 did not have any aroma peculiar to allyl isothiocyanate.

Example 8 200 mg of allyl isothiocyanate was added to a filter paper (Toyo Roshi)
Co., Ltd. filter paper No. 1034-3A, 3 mm x 40 mm x 7
0 mm) to obtain a sample according to the present invention.

Example 9 After dissolving 200 mg of allyl isothiocyanate in 500 mg of honeydew, filter paper (filter paper No. 1034 manufactured by Toyo Roshi Co., Ltd.) was dissolved.
-3A, 3 mm x 40 mm x 70 mm) to obtain a sample according to the present invention.

Comparative Example 3 A sample of Comparative Example 3 was prepared by placing 200 mg of allyl isothiocyanate in a glass Petri dish with an inner diameter of 90 mm.

The samples of Examples 8 and 9 and Comparative Example 3 were allowed to stand at room temperature and the degree of release (%) of allyl isothiocyanate was examined, as shown in FIG.
Samples No. 9 and 9 exhibited suppressed release of allyl isothiocyanate compared to the sample of Comparative Example 3. In particular, a remarkable sustained release effect was observed in the sample of Example 9.

Using the same filter paper as that obtained in Example 8,
The following tests were conducted. In other words, five male adults who had been suffering from athlete's foot caused by Trichophyton on their fingers and toes for more than two years were given one patch on each side of the inside of the shoes they use on a daily basis. The patient was inserted and allowed to live the same life as before. The filter paper was replaced with a new one every 3 days and was used for 15 days. As a result, 3 out of 5 subjects were completely cured of athlete's foot in their toes, and no recurrence of athlete's foot was observed for more than three months after the end of the test. Regarding the remaining two patients, one was expected to be completely cured because the pain and itching caused by athlete's foot were eliminated and the eczema disappeared during the test period, but the itching and eczema returned one week after the test ended. Furthermore, one person experienced relief from the pain and itching of athlete's foot only during the test period. Regarding the unpleasant odor of the feet, the three subjects who made a complete recovery disappeared from the third to fifth day after the start of the test. The remaining two patients had also disappeared by the end of the test. This test confirmed that the antibacterial preparation according to the present invention is effective in removing athlete's foot and unpleasant foot odor associated with athlete's foot.

[0026]

Effects of the Invention As described above, the present invention is easy to use by attaching and retaining allyl isothiocyanates, which have a preservative effect as a gas when vaporized, on a solid fibrous support such as a fibrous mat or filter paper. , the liquid allyl isothiocyanate does not directly adhere to the object to be preserved, and the allyl isothiocyanate is attached to the support, so that the degree of adhesion and the density of the mat, paper, etc. are selected, and By selecting the temperature, it is possible to control the vaporization rate, and if the characteristic odor of allyl isothiocyanate becomes a nuisance, we provide an antibacterial material-adhesive preparation that suppresses it by using ethanol together. can do.

[Brief explanation of the drawing]

FIG. 1 is a perspective view schematically showing an experimental apparatus for confirming the effects of the present invention.

FIG. 2 is a graph showing the degree of release of allyl isothiocyanate over time from samples at various concentrations at 0° C. for Example 1.

FIG. 3 is a graph showing the degree of release of allyl isothiocyanate over time from samples at various concentrations at room temperature for Example 1.

FIG. 4 is a graph showing the degree of release of allyl isothiocyanate over time from samples at various concentrations at 40° C. for Example 1.

FIG. 5 is a graph showing the degree of release of allyl isothiocyanate over time for each sample of Examples 2 to 4 and Comparative Example 1.

FIG. 6 is a graph showing the degree of release of allyl isothiocyanate over time for each sample of Examples 5 to 7 and Comparative Example 2.

FIG. 7 is a graph showing the degree of release of allyl isothiocyanate over time for each sample of Examples 8 and 9 and Comparative Example 3.

[Explanation of symbols]

1: Glass bottle 2: Stirring blade 3: Stirrer 4: Stopper 5: Thread 6: Matt

Claims (5)

[Claims] Claim 1: An antibacterial substance consisting of allyl isothiocyanate or an oil-soluble extract containing allyl isothiocyanate obtained from wasabi, wasabi radish, or mustard, which are plants of the cruciferous family, is adhered to a fibrous support. An antibacterial substance-attached preparation characterized by: [Claim 2] A fatty acid ester compound of an antibacterial substance consisting of an oil-soluble extract containing allyl isothiocyanate or allyl isothiocyanate obtained from wasabi, wasabi radish, or mustard, which are plants of the cruciferous family, on a fibrous support; An antibacterial substance adhesion preparation characterized by adhering a liquid dissolved in paraffin. [Claim 3] An antibacterial substance consisting of an oil-soluble extract containing allyl isothiocyanate or allyl isothiocyanate obtained from wasabi, wasabi radish, or mustard, which are plants of the cruciferous family, or dissolved in a fatty acid ester compound or paraffin. Claim 1: The antibacterial substance obtained by adding ethanol to a liquid is adhered to a fibrous support.
or the antibacterial substance-attached preparation described in 2. 4. The antibacterial preparation preparation adhering body according to claim 1 or 2, wherein the fibrous support is composed of pulp. [Claim 5] The fatty acid ester compound is glycerin fatty acid ester, sorbitan fatty acid ester, phospholipid,
The antibacterial substance adhesion preparation according to claim 2 or 3, which is a compound selected from higher alcohol fatty acid esters alone or in combination.