JPH04299974A - Device for culturing plant requiring light - Google Patents

Abstract

PURPOSE:To provide a culture device for a light-requiring plant to be cultured, capable of effectively and uniformly irradiating the light on the culturing plant a stable culture operation. CONSTITUTION:A luminous cylinder 2 which comprises a cylinder provided with at least one of a layer for reflecting light from an artificial light source 3 and a layer for diffusing the light and which reduces the attenuation of the light in the axial direction of the cylinder to uniformly irradiate the light on the outside of the cylinder is disposed in a culture chamber 1, and the light is irradiated in the culture solution 4 in the culture layer 1 from the luminous cylinder 2. If necessary, a liquid or gas can be injected into or sealed in the luminous cylinder 2 from a liquid or gas supplying system 7 to control the temperature of the culture solution 4. The artificial light source 3 and the luminous cylinder 2 are integrally vertically moved by a stand 8 to control the depth of the luminous cylinder 2 inserted into the culture 4.

Description

[Detailed description of the invention]

[Industrial Application Field] The present invention relates to an apparatus for cultivating a cultured material that requires light, and in particular, the present invention relates to an apparatus for cultivating a cultured material that requires light.
The present invention also relates to a culture device suitable for uniformly irradiating light.

[Prior Art] When plants perform photosynthesis at a higher rate than in nature and cultivate bacteria, algae, etc., light sources commonly used include sunlight, fluorescent lamps, halogen lamps,
Culture devices using mercury lamps and the like have been used or proposed. FIGS. 8 and 9 show conventional examples of culturing apparatuses for culturing objects that require such light. FIG. 8(A) shows a structure in which a culture tank 31 made of a material that transmits light is covered with a light supply part 32 made of a fluorescent lamp, a halogen lamp, a mercury lamp, etc. installed on a support part 33. 31 and the light supply section 32 are completely separated. FIG. 8(B) shows that after condensing light obtained from a light source (not shown) into the culture tank 34,
The end face of the optical fiber 35 for transmission is arranged, and the culture solution 36 is irradiated with light from this end face. FIG. 9 shows a protection tube 38 that penetrates the center of the culture tank 37.
A fluorescent lamp 41 connected to an AC power source 40 is inserted into the protective tube 38, and light is transmitted from the fluorescent lamp 41 through the protective tube 38 to the culture solution 39 in the culture tank 37 outside the protective tube 38. It is irradiating.

[Problems to be Solved by the Invention] In the case of a culture apparatus as shown in FIG. It is difficult to irradiate light efficiently and uniformly,
Furthermore, the culture tank 31 needs to be made of a material that transmits light, which poses a practical problem in large culture apparatuses. Also,
In the culture apparatus shown in FIG. 8(B), the culture solution 3
In order to irradiate the entire optical fiber 6 with light, the diameter of the bundle of optical fibers 35 must be made large enough to correspond to the culture tank 34. However, the optical fiber 35 is difficult to process, and the cost increases due to its large capacity.Moreover, it is difficult to remove the material to be cultured that has adhered to the light emitting part of the optical fiber 35, so continuous culturing operations are not possible. It becomes difficult. Furthermore, in the case of the culture apparatus shown in FIG. 9, it is difficult to achieve uniform irradiation using the fluorescent lamp 41, and the intensity of the fluorescent lamp 41 is low.
As a result of requiring the protection tube 38, structural restrictions arise on the culture tank 37. The purpose of the present invention is to solve the above-mentioned problems of the prior art, to be able to be applied to large-scale culture equipment without causing structural restrictions on culture tanks, and to be able to It is an object of the present invention to provide a culturing device for a cultured material that requires light, which can efficiently and uniformly irradiate light to perform stable cultivation of the cultured material.

[Means for Solving the Problems] In order to achieve the above object, the present invention includes a cylindrical body provided in a culture tank with at least a layer that reflects light from a light source and a layer that diffuses light. A light-emitting cylinder is arranged to reduce the attenuation of light in the axial direction and uniformize the light irradiated to the outside of the cylinder, and the light is irradiated to the cultured material in the culture tank through the light-emitting cylinder. The structure is as follows. Further, the light emitting cylinder is installed movably with respect to the culture tank, and the length of the light emitting cylinder inserted into the culture solution in the culture tank can be adjusted. Furthermore, a filter that selectively transmits light in a specific wavelength range is arranged between the light source section and the light emitting cylinder, and the end of the light emitting cylinder on the culture tank side is closed, so that liquid or liquid inside the light emitting cylinder is closed. Introduce gas,
The structure is provided with a means for discharging the liquid or gas inside the light emitting cylinder and adjusting the temperature of the liquid in the culture tank by the light emitting cylinder. In addition, a rotating shaft having a stirring blade is provided in the culture tank, at least a part of the stirring blade and the rotating shaft are made of a light-emitting cylinder, and a plurality of pores are formed in the light-emitting cylinder. It is equipped with means for supplying gas to the inside and discharging the gas from the pores.

[Function] The culture tank consists of a cylindrical body with at least a layer that reflects the light from the light source and a layer that diffuses it, and reduces the attenuation of the light in the axial direction and irradiates the outside of the cylindrical body. By arranging the light-emitting tube that makes the light uniform, the light-emitting tube emits light uniformly, and the culture solution in the culture tank can be uniformly irradiated with light. The structure allows the luminous cylinder to be movably installed relative to the culture tank and adjusts the length of the luminous cylinder inserted into the culture solution in the culture tank, so that the luminous cylinder can be inserted into the culture medium while emitting light. can be inserted or pulled out. By partitioning the light source section and the light emitting tube and placing a filter between them that selectively transmits light in a specific wavelength range, the light emitting tube is divided into the light emitting section and the light source section, and the heat of the light source section is reduced. The influence of sunlight on the light emitting part is reduced, and sunlight can be supplied to the inside of the culture solution, seawater, etc. without using optical fibers, and a large-scale culture apparatus becomes possible. Closing the end of the luminescent cylinder on the culture tank side, introducing liquid or gas into the luminous cylinder, and discharging the liquid or gas inside the luminous cylinder to adjust the temperature of the liquid in the culture tank by the luminous cylinder. This makes it possible to adjust the temperature of the culture solution to the most suitable temperature for the culture, allowing efficient and stable culture operations. When a rotating shaft having a stirring blade is provided in the culture tank, and at least a portion of the stirring blade and the rotating shaft are formed of a light emitting cylinder,
The culture solution can be uniformly irradiated with light while stirring the culture solution, enabling efficient culture and simplifying the apparatus. Supplying gas such as carbon dioxide necessary for culture into the culture solution by forming multiple pores in the luminous cylinder, supplying gas inside the luminous cylinder, and discharging the gas from the pores. can.

Embodiments Hereinafter, embodiments of the present invention will be explained based on the drawings. FIG. 1(A) shows a culture apparatus showing a first embodiment of the present invention. In FIG. 1(A), a light emitting cylinder 2 is placed in the center of a culture tank 1, and this light emitting cylinder 2 is closed at its tip (end on the side of the culture tank 1) and at its rear end. The section is open and connected to the artificial light source section 3 via a filter 6. The filter 6 has the function of converting the wavelength of light or selectively supplying light in a specific wavelength range. As the filter 6, a glass filter or a group 2a-5b compound, for example, a SrS-based compound is suitable. When a SrS-based compound is used in a filter, only a specific excitation wavelength is selectively shifted to a different wavelength, and light is emitted.
Since the energy of the light of the emitted wavelength increases, when the same light source is used, it is possible to increase the energy of any wavelength required for the cultured material. Further, in the case of a glass filter, only the target wavelength is transmitted, but a plurality of glass filters may be installed as necessary. The material of the light emitting cylinder 2 is preferably hydrophobic, since the light emitting cylinder 2 is inserted into a culture solution, seawater, etc., and preferably one that does not denature by steam treatment. Further, since gas and liquid are injected inside the light emitting cylinder 2, it is desirable that the material of the internal diffusion material etc. is heat resistant and pressure resistant. A culture solution 4 is stored in the culture tank 1, and a cultured material 5 is dispersed within the culture solution 3. In the figure, 7 indicates the gas/liquid supply system,
Gas or liquid is supplied or sealed into the artificial light source section 3 or the light emitting cylinder 2. Furthermore, the light emitting cylinder 2 and the artificial light source section 3 are integrally movable up and down by a stand 8. This moving mechanism allows the length of the light emitting cylinder 2 inserted into the culture tank 1 to be adjusted. Here, as the above-mentioned liquid, for example, water or the like is used to control the temperature of the light-emitting cylinder 2 and/or the artificial light source section 3, and as the above-mentioned gas, air or the like is used to control the temperature of the light-emitting cylinder 2 and/or the artificial light source section 3.
and/or used for temperature control of the artificial light source section 3. In such a culture device, the light emitting cylinder 2 is entirely made of glass, and one side of the cylinder is open and the other side is closed. The interior of the light-emitting cylinder 2 is coated with a diffuse reflection material, and by attaching a light source to the open side of the cylinder, the cylinder emits light uniformly. Therefore, when such a light-emitting cylinder 2 is used, it can be inserted into or removed from the culture solution 4 in a state where light is emitted, so that it becomes easy to clean the cultured object that has adhered to the light-emitting part. FIG. 1(B) is a configuration diagram showing a side and top plan view of a culture apparatus showing a second embodiment of the present invention. In this culture apparatus, a filter 6 is installed at the upper end of a light emitting cylinder 2 arranged in the center of a culture tank 1, and a longitudinal end of an optical fiber 9 is connected to this filter 6. In this culture device, sunlight is collected, transmitted through the optical fiber 9, and emitted from the inside of the light emitting cylinder 2, so the light emitting area is expanded compared to the case where the light is emitted directly from the cross section of the optical fiber 9. , the culture efficiency will be improved. In FIG. 1(B), a filter 4 is installed between the light-emitting cylinder 2 and the optical fiber 9, but this filter 9 is not necessarily provided, and only light with a wavelength suitable for the cultured material is used. In the case of a light source for irradiation, the optical fiber 9 can be omitted. FIG. 2(A) shows a culture apparatus showing a third embodiment of the present invention. This culture device uses a light emitting cylinder 2 at the shaft of a stirring blade 10 installed in the center of a culture tank 1. In this culture device, the culture solution 4 is stirred by the motor 11 and light is evenly supplied radially from the light emitting cylinder 2, so that the culture effect is high. Note that the light emitting cylinder 2 may constitute at least a part of the stirring blade 10 and its shaft portion depending on the amount of culture solution in the culture tank. Figure 2 (
B) is a culture device showing a fourth embodiment of the present invention. In this culture apparatus, a light-emitting cylinder 2 installed at the center of a culture tank 1 can be moved up and down in the axial direction of the light-emitting cylinder 2 by an elevator 12. Therefore, in this device, the length of the light emitting cylinder 2 inserted into the culture medium 4 can be arbitrarily selected, so that the amount of light supplied to the cultured object in the culture medium 4 can be adjusted. The growth rate can be controlled by Furthermore, since the light-emitting tube 2 can be extracted from the culture solution 4 without removing the culture solution 4 from the culture tank 1, it becomes easy to clean the cultured matter attached to the light-emitting tube 2. 3, 4 and 5 show a culture device showing a fifth embodiment of the present invention, and FIG. 4 is a detailed internal view of FIG.
FIG. 5 is a plan view of FIG. 4. This culture device consists of culture tank 1
A cylindrical opening is formed in the center of the upper part, and the light emitting cylinder 2 is inserted into this opening and a seal cap 13 is applied with a packing 14 interposed therebetween. A ring-shaped light-emitting cylinder support part 17 is provided on the upper side of the light-emitting cylinder 2, and a ring-shaped synthetic resin ring 18 is interposed in this light-emitting cylinder support part 17, and is fixed to the light-emitting cylinder 2 with bolts 19. has been
A ring 15 having a gas injection hole 15a is provided in the light emitting cylinder support part 17 so as to communicate with the internal space 2a of the light emitting cylinder 2. Further, two gas exhaust holes 16 are bored in the light emitting cylinder support portion 17 . In the artificial light source section 3, as shown in FIG. 4, a filter 21 is fixed to a filter support section 22 on the upper end surface of a light emitting cylinder support section 17, and above the filter 21 a lamp 23 is fixed to a hemispherical reflector plate 24. is installed inside. The lamp 23 is connected to a voltage regulator or the like via a cable 25. Further, a fan 20 is installed on the side wall surface of the artificial light source section 3. In the above-mentioned culture device, gas is injected from the gas inlet 15a through the nozzle 15 into the internal space 2a of the light emitting tube 2, and this gas reaches the surface of the tip 2c of the light emitting tube, and the gas is discharged. It is discharged from the hole 16 to the outside of the cylinder. By controlling the temperature, amount, etc. of the gas supplied to the internal space 2a of the light emitting cylinder 2 in this way, the temperature of the culture solution 4 can be controlled and adjusted to a temperature suitable for culture. Further, by forming the pores 2b in the circumferential side surface of the light emitting cylinder 2, gas can be supplied from the inside of the light emitting cylinder 2 into the culture solution 4. Examples of the gas supplied into the culture medium from this pore 2b include CO2, air + CO2, N2, N2 + air, etc. When using gases such as CO2, air + CO2, etc., it is necessary to control the temperature of the culture medium at the same time. Promotes photosynthetic reactions in cultures. In addition, the light emitted from the lamp 23 is almost uniformly irradiated in the axial direction of the light emitting cylinder 2 through the hemispherical reflecting plate 24, and this light is uniformly irradiated from the light emitting cylinder 2 to the culture medium 4. be done. Furthermore, as shown in FIG. 3, when fixing the light emitting cylinder 2, the bolt 19 is
This prevents the light emitting cylinder 2 from being damaged during the work of fixing the light emitting cylinder 2. Furthermore, lamp 13
It is possible to prevent heat accumulation in the casing constituting the artificial light source section 3 by the light irradiation from the artificial light source section 3. Although the lamp 23 is provided in FIG. 3, it goes without saying that an optical fiber may be provided instead of the lamp 23. FIG. 6 shows the basic principle of the light emitting cylinder in the present invention,
The light emitting cylinder basically consists of a light source section and a light emitting section. The light emitted from the light source travels along the axial direction of the pipe while being reflected within the pipe, as shown by arrow A in the figure, within the light emitting unit. FIG. 7 is an explanatory diagram showing the structure, material, and function of the light emitting cylinder. As shown in FIG. 7, the light emitting cylinder 2 has a structure in which an extractor 26, a prism light guide 27, a diffusion sheet 28, a diffusion film 29, and a housing 30 are laminated in order from the inside thereof. The extractor 1a is made of a polyethylene sheet or the like, and is provided to transmit the light taken in from the light source and supply the light to the prism light guide 27. The prism light guide 27 is made of acrylic resin or the like, and reflects the light beam that has passed through the extractor 26 to change the direction, or uses birefringence to disperse the light, and sends it to the diffusion sheet 28. Provided to transmit light. The diffusion sheet 28 is formed from a polyethylene sheet or the like, and is provided to diffuse (disperse) the beam of light that has passed through the prism light guide 27 and transmit the light to the outer peripheral portion. The diffusion film 29 is formed from a polyester film or the like, and is provided to diffuse (disperse) the beam of light that has passed through the diffusion sheet 29 in this portion and transmit the light to the outer peripheral portion. The housing 30 functions to protect the internal laminated structure and transmit light to the outside, and is made of, for example, acrylic resin, glass, or the like. As mentioned above, the light (ray) that enters the inside of the cylinder from the light source of the light emitting cylinder 2 is divided into two types: light that travels while being reflected in the axial direction inside the cylinder (A in Fig. 6), and light that exits outside the cylinder (A). It is roughly divided into B and C) in FIG. Considering these optical paths and light amounts, in order to uniformly irradiate light to the outside from all surfaces of the light emitting cylinder 1, (1) the type, thickness, and number of laminated materials of the internal laminated portion in the axial direction; (2) The shape, apex angle, pitch number, prism angle, prism height, etc. of the prism light guide 27 shown in FIG. 7 are changed in the axial direction. This internal structure is arbitrarily changed to mainly prevent light attenuation in the axial direction. Therefore, the light from the light emitting cylinder 2 is uniformly irradiated in the axial direction.

As described above, according to the present invention, (1) the growth rate of the cultured material is improved, and the light utilization rate for CO2 fixation by the cultured material is improved, (2) the culture tank is (3) Light source section and light emitting section By installing a filter between the two parts, it is possible to variably supply light in a specific wavelength range to the cultured material.
4) Compared to the conventional example in which light is transmitted through an optical fiber and emitted light from its end face, the light emitting area is increased and the work of peeling the cultured material is easier. (5) Either the rotating shaft or the stirring blade is used. When configured with a light emitting cylinder, the light emitting part rotates and emits light, so that it is possible to perform uniform light emitting operation and at the same time prevent attachment of cultured objects. (6) Structure that separates the light source part and the light emitting part By doing this, it is possible to prevent the temperature of the culture solution from rising. (7) By making the light-emitting tube body removable from the culture solution, it becomes easy to peel off and wash the cultured matter that adheres to the light-emitting part. , (8) Compared to fluorescent lamps, the light-emitting cylinder prevents the culture medium from generating heat and makes it easier to adjust the illuminance.
(9) The temperature of the culture medium can be adjusted by having a structure that allows gas or liquid to be supplied inside the light emitting cylinder, and by supplying gas from the inside of the light emitting cylinder into the culture medium, the gas necessary for culture can be supplied. can be supplied, improving culture efficiency.

[Brief explanation of drawings]

FIG. 1 (A) is a schematic configuration diagram of a culture device showing a first embodiment of the present invention, and (B) is a schematic configuration diagram showing a second embodiment of the culture device of the present invention.

FIG. 2: (A) is a schematic configuration diagram showing a third embodiment of the culture device of the present invention, and (B) is an explanatory diagram showing the fourth embodiment of the culture device of the present invention, showing the operating state of the device. It is.

FIG. 3 is a schematic configuration diagram showing a fifth embodiment of the culture apparatus of the present invention.

FIG. 4 is a detailed internal view of the artificial light source section in FIG. 3;

FIG. 5 is a bottom view of FIG. 4;

FIG. 6 is an explanatory diagram showing the basic principle of a light emitting cylinder.

FIG. 7 is an explanatory diagram showing the structure, material, and function of the light emitting cylinder.

FIG. 8 (A) is an explanatory diagram showing a conventional culture device, (B)
1 is a schematic configuration diagram showing a conventional culture device.

FIG. 9 is a schematic configuration diagram showing a conventional culture device.

[Explanation of symbols]

1 Culture tank 2　Light emitting cylinder 3 Artificial light source section 4 Culture solution 5. Cultured material 6 Filter 7 Air/liquid supply system 8 Stand 9 Optical fiber 10 Stirring blade 11 Motor 12 Elevator 13 Seal cap 14 Packing 15 Nozzle 16 Gas exhaust hole 17 Light emitting cylinder support part 18 Synthetic resin ring 19 bolts 20 Fan 21 Filter 22 Filter support part 23 Lamp 24 Reflector plate 25 Cable

Claims (5)

[Claims] Claim 1: A cylindrical body provided in a culture tank with at least a layer that reflects light from a light source and a layer that diffuses the light, and irradiates the outside of the cylindrical body while reducing attenuation of light in the axial direction. A cultured object that requires light, characterized in that a light-emitting cylinder is arranged to uniformize the light emitted, and the cultured object in the culture tank is irradiated with light through the light-emitting cylinder. culture equipment. 2. A claim characterized in that the light emitting cylinder is movably installed with respect to the culture tank, and the length of the light emitting cylinder inserted into the culture solution in the culture tank can be adjusted. Item 1
A culturing device for cultured materials that requires light. 3. The cultured object requiring light according to claim 1, further comprising a filter that selectively transmits light in a specific wavelength range between the light source section and the light emitting cylinder. Culture device. 4. The culture tank side end of the light emitting cylinder is closed, and a liquid or gas is introduced into the light emitting cylinder, and the liquid or gas inside the light emitting cylinder is discharged. 4. The apparatus for cultivating something to be cultured that requires light according to claim 1, further comprising means for adjusting the temperature of the liquid in the culture tank.Claim 4: A rotating shaft having a stirring blade in the culture tank. 2. The apparatus for cultivating a cultured material requiring light according to claim 1, wherein at least a part of the stirring blade and the rotating shaft are constituted by the light emitting cylinder. 5. The cultivated material requiring light according to claim 4, wherein a plurality of pores are formed in the light emitting cylinder and means for supplying gas into the inside of the light emitting cylinder is provided. culture equipment.