JPH04207160A - Method for removing nitrate nitrogen in crude feed, horticultural food or the like - Google Patents
Method for removing nitrate nitrogen in crude feed, horticultural food or the likeInfo
- Publication number
- JPH04207160A JPH04207160A JP2338615A JP33861590A JPH04207160A JP H04207160 A JPH04207160 A JP H04207160A JP 2338615 A JP2338615 A JP 2338615A JP 33861590 A JP33861590 A JP 33861590A JP H04207160 A JPH04207160 A JP H04207160A
- Authority
- JP
- Japan
- Prior art keywords
- nitrate nitrogen
- yeast
- food
- nitrate
- horticultural
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 title claims abstract description 25
- 235000013305 food Nutrition 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 17
- 108090000790 Enzymes Proteins 0.000 claims abstract description 4
- 102000004190 Enzymes Human genes 0.000 claims abstract description 4
- 235000013325 dietary fiber Nutrition 0.000 claims description 11
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 210000003850 cellular structure Anatomy 0.000 claims description 5
- 101710136001 Assimilatory nitrate reductase Proteins 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 8
- 229910002651 NO3 Inorganic materials 0.000 abstract description 4
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 abstract description 4
- 241000235648 Pichia Species 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract 2
- 210000005253 yeast cell Anatomy 0.000 abstract 2
- 239000003183 carcinogenic agent Substances 0.000 abstract 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 14
- 239000000126 substance Substances 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 10
- 238000012360 testing method Methods 0.000 description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 7
- 235000014655 lactic acid Nutrition 0.000 description 7
- 239000004310 lactic acid Substances 0.000 description 7
- 208000005374 Poisoning Diseases 0.000 description 5
- 230000000711 cancerogenic effect Effects 0.000 description 5
- 231100000315 carcinogenic Toxicity 0.000 description 5
- 231100000572 poisoning Toxicity 0.000 description 5
- 230000000607 poisoning effect Effects 0.000 description 5
- 208000024891 symptom Diseases 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 235000009508 confectionery Nutrition 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 244000025254 Cannabis sativa Species 0.000 description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 244000000626 Daucus carota Species 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 108090000913 Nitrate Reductases Proteins 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- 241000269400 Sirenidae Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000300264 Spinacia oleracea Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000005757 colony formation Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 150000002823 nitrates Chemical class 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 235000021110 pickles Nutrition 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 235000013324 preserved food Nutrition 0.000 description 1
- 239000004460 silage Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Fodder In General (AREA)
- General Preparation And Processing Of Foods (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、粗飼料、園芸食品等に含有され、家畜や人が
これを摂取すると体内で他の物質と化合して中毒症状を
呈したり、また、発ガン性物質を生成したりする硝酸態
窒素を除去するようにした粗飼料、園芸食品等における
硝酸態窒素の除去方法に関するものである。[Detailed Description of the Invention] [Industrial Application Field] The present invention is a compound that is contained in roughage, horticultural food, etc., and when livestock or humans ingest it, it combines with other substances in the body and causes poisoning symptoms. The present invention also relates to a method for removing nitrate nitrogen from roughage, horticultural foods, etc., which removes nitrate nitrogen, which produces carcinogenic substances.
従来、牧草のような粗飼料、また、野菜のような園芸食
品中には、硝酸、硝酸塩等の硝酸態窒素を含有している
ことが知られている。そして、これらを家畜や人が摂取
すると体内で亜硝酸となって他の物質と化合して中毒症
状を起こしたり、また、発ガン性物質を生成したりする
ことも知られている。It has been known that roughage such as grass and horticultural foods such as vegetables contain nitrate nitrogen such as nitric acid and nitrates. It is also known that when livestock or humans ingest these substances, they turn into nitrous acid in the body and combine with other substances, causing poisoning symptoms and producing carcinogenic substances.
ところが、上記のように家畜や人が摂取すると体内で亜
硝酸となり、他の物質と化合して中毒症状を起こしたり
、また、発ガン性物質を生成したりする元凶となる硝酸
態窒素を、粗飼料、園芸食品等から積極的に除去する技
術は開発されていないのが現状である。However, as mentioned above, when livestock or humans ingest it, it becomes nitrite in the body, and nitrate nitrogen is the culprit that can combine with other substances to cause poisoning symptoms and produce carcinogenic substances. Currently, no technology has been developed to actively remove it from roughage, garden foods, etc.
本発明は、上記の事情に鑑みなされたもので、粗飼料、
園芸食品等から体内に入ってから有害物質となる硝酸態
窒素を簡易に除去できる方法を提供することを目的とす
る。The present invention was made in view of the above circumstances, and includes roughage,
The purpose of the present invention is to provide a method for easily removing nitrate nitrogen, which becomes a harmful substance after entering the body from gardening foods, etc.
上記の目的を達成するために本発明は、硝酸態窒素を含
有している粗飼料、園芸食品等に、同化型硝酸塩還元酵
素を有する酵母を接種し、この酵素を利用して硝酸態窒
素を還元後、さらに菌体成分に変換し、食・飼料等から
硝酸態窒素を除去するようにしたことを特徴とするもの
である。In order to achieve the above object, the present invention inoculates roughage, horticultural food, etc. containing nitrate nitrogen with yeast having an assimilative nitrate reductase, and uses this enzyme to reduce nitrate nitrogen. After that, it is further converted into bacterial cell components to remove nitrate nitrogen from food, feed, etc.
上記の方法によって、硝酸態窒素は、酵母によって還元
され、さらに菌体成分に変換されて消失し、中毒症状を
起こしたり、発カン性物質を生成したりしない。By the above method, nitrate nitrogen is reduced by the yeast and further converted into bacterial cell components and disappears, and does not cause poisoning symptoms or produce carcinogenic substances.
以下、図面および別表を参照して、本発明をスィートツ
ルガムを材料として用いた実施例について説明する。EXAMPLES Hereinafter, embodiments of the present invention using sweet turgid gum as a material will be described with reference to the drawings and attached tables.
（１）実施手法
［使用菌株］
酵母は、保存・収集株、分離株針２０株強の中からサイ
レージに常在的なピキア（Ｐｉｃｈｉａ）属菌柱て゛、
硝酸塩同化能、蔗糖同化能か比較的強いキラー株を使用
した。乳酸菌は、ラクトバチルス プランタルム（Ｌａ
ｃｔｏｂａｃｉｌｌｕｓ ｐｌａｎｔａｒｕｍ）ＫＴ
Ｃ４−３株を用いた。酵母は、酵母エキスＣＦ、 １
０６加用ツアペツクドツクス（Ｃｚａｐｅｃ Ｄｏｘ
）培地にて３日間振盪培養後、乳酸菌は同じ＜：ＭＲ５
培地にて２日間培養後、表１に示す接種菌数（ｉｎｏｃ
ｕｌｕｍ ５ｉｚｅ）て接種した。(1) Implementation method [Bacterial strains used] Yeasts include Pichia genus bacteria, which are resident in silage, from among over 20 preserved and collected strains and isolated needle strains.
A killer strain with relatively strong nitrate assimilation ability and sucrose assimilation ability was used. Lactic acid bacteria are Lactobacillus plantarum (La
ctobacillus plantarum) KT
C4-3 strain was used. Yeast is yeast extract CF, 1
06 added Czapec Dox
) After 3 days of shaking culture in the medium, the lactic acid bacteria were the same <: MR5
After culturing in the medium for 2 days, the number of inoculated bacteria shown in Table 1 (inoc
ulum 5ize) and inoculated.
［材 料］
実験］、２（予備試験）では、圃場にて栽培したスィー
トツルガムを材料とし、微切断後、３木ロール型搾ｌＩ
−機にて茎部のみの残渣を調製後、−処理区当り新鮮物
１０ｋｇ、同しく実験３（本試験）では、別に調製した
残渣を１４０ｋｇ／区供試した。[Materials] Experiment] In 2 (preliminary test), sweet vine gum grown in the field was used as the material, and after finely cutting, it was pressed into a three-wood roll type.
- After preparing the residue of only the stem in a machine, - 10 kg of fresh material was prepared per treatment plot. Similarly, in Experiment 3 (main test), 140 kg of separately prepared residue was tested per plot.
その際、実験１ては、硝酸カリウムをＮＯ３として０．
４９６／新鮮物、同しく実験２，３ては０．２％補足し
た。At that time, in Experiment 1, potassium nitrate was used as NO3 and 0.
496/fresh, also supplemented with 0.2% in Experiments 2 and 3.
［処理区］ ３回の実験とも表２に示す４処理区により実施した。[Treatment area] All three experiments were conducted using the four treatment groups shown in Table 2.
［前処理工程］
酵母および乳酸菌を接種後の残渣は、予備試験ては厚さ
３． ５ｃｍ、同しく本試験では１５ｃｎ＋にて室内に
２日間堆積した。この間３〜５回切り返しく第１図参照
）を行った後、材料はサイロに詰めた。[Pretreatment Step] In a preliminary test, the residue after inoculating yeast and lactic acid bacteria had a thickness of 3.5 mm. 5 cm, also in this test 15 cn+, was deposited indoors for 2 days. During this period, the material was turned back and forth 3 to 5 times (see Figure 1), and then packed into a silo.
［分析・測定法等コ
水分は凍結乾燥法、硝酸態等はイオンクロマトグラフ法
およびブレムナ−（Ｂ ｒ ｅｍｎ ｅ ｒ）法の併用
により、ｐｈ等の物理・化学的測定は慣行の方法、微生
物数の計算等は希釈平板により行った。[Analysis/Measurement Methods] Moisture was measured by freeze-drying, nitrate was measured using a combination of ion chromatography and Bremner methods, and physical and chemical measurements such as pH were performed using conventional methods and microorganisms. Calculations, etc. were performed using dilution plates.
（２）実施結果
■、予備試験では、接種２日後、酵母接種区（３゜４区
）の品温は、酵母無接種区（１，２区）に比較し約３℃
高温にあった。また、本試験においては、接種約１６時
間後より品温の上昇か認められた。特に酵母と乳酸菌を
併用した４区で発熱が顕著であった（第１図参照）。(2) Results■, In the preliminary test, two days after inoculation, the product temperature in the yeast inoculated area (3°C area 4) was about 3℃ compared to the yeast non-inoculated area (1st and 2nd areas).
It was at a high temperature. Furthermore, in this test, an increase in product temperature was observed approximately 16 hours after inoculation. Particularly, fever was remarkable in the 4 plots where yeast and lactic acid bacteria were used together (see Figure 1).
■、３回の実験とも、乳酸菌接種区（２，４区）におい
て乳酸菌数の顕著な増加は認められなかった。一方、酵
母は著しい増殖を示し、３，４区て８．８８〜Ｑ、６７
ｃ ｆ ｕ／ｇ （常用対数表示、集落形成単位）に達
した（表２参照）。(2) In all three experiments, no significant increase in the number of lactic acid bacteria was observed in the lactic acid bacteria inoculated areas (groups 2 and 4). On the other hand, yeast showed remarkable growth, with 8.88 to 67 in the 3rd and 4th plots.
c f u/g (common logarithmic expression, colony formation unit) was reached (see Table 2).
■ いずれの実験においても、酵母接種区では、酵母無
接種区に比較し、硝酸態窒素（ＮＯ３）の消失が顕著で
あり、特に予備試験では６６．５〜７６．２％と、酵母
無接種区の約７倍も高い消失を示した（表２参照）。■ In both experiments, the loss of nitrate nitrogen (NO3) was remarkable in the yeast-inoculated plot compared to the yeast-uninoculated plot, especially in the preliminary test, it was 66.5 to 76.2%, which was 66.5% to 76.2% in the yeast-inoculated plot. The loss was approximately 7 times higher than in the ward (see Table 2).
■、２日間を通し、いずれの材料においても亜硝酸（Ｎ
Ｏ２）は不検出（第２図参照）であり、アンモニア態窒
素も不検出〜極微量であった。また、酵母菌はこの間に
顕著なｃｆｕの増加をみていることにより、酵母接種区
で消失したＮＯ３はそのまま菌体成分へ変換・除去され
たといえる。■Throughout the two days, nitrite (N
O2) was not detected (see Figure 2), and ammonia nitrogen was also not detected to a very small amount. Furthermore, since the cfu of yeast bacteria significantly increased during this period, it can be said that the NO3 that disappeared in the yeast inoculated area was directly converted and removed into bacterial cell components.
なお、サイロは未開封であるが、ＰＶＣサイロへ詰めて
調製したサイレージ（予備試験）では、外観上いずれの
サイロでも、不良発酵の発生は感知されていない。Although the silos are unopened, no defective fermentation has been detected in any of the silages prepared by filling them in PVC silos (preliminary test).
この試験結果から、本発明の硝酸態窒素の除去方法は、
牧草や飼料用長大作物（粗飼料）およびそれらを発酵、
または乾燥して調製するサイレーンや乾苧と同様に硝酸
態窒素を含有するホウレンソウ、トマト、ニンジン、タ
イコン等の園芸食品およびそれらを加工する漬物や乾物
にも同様に適用できるものである。From this test result, the nitrate nitrogen removal method of the present invention is as follows:
Grass and long crops for feed (roughage) and fermenting them,
It can also be applied to horticultural foods, such as spinach, tomatoes, carrots, and radish, which contain nitrate nitrogen, as well as sirens and dried ramets prepared by drying, as well as pickles and dried foods processed from them.
以上説明したように本発明の粗飼料、園芸食品等におけ
る硝酸態窒素の除去方法によれば、硝酸態窒素を含有す
る粗飼料、園芸食品等に対し硝酸塩同化能を有する酵母
を接種するたけて硝酸態窒素を菌体成分に変換し、消去
、除去できるので、その作業は簡単であり、従来、体内
に入って中毒症状を起こしたり、発ガン性物質を生成し
ていた危険を、未然に防止することができる。As explained above, according to the method for removing nitrate nitrogen from roughage, horticultural food, etc. of the present invention, yeast having the ability to assimilate nitrate is inoculated into roughage, horticultural food, etc. containing nitrate nitrogen. Nitrogen can be converted into bacterial cell components, erased, and removed, so the process is simple and prevents the dangers that traditionally occur when entering the body, causing poisoning symptoms or producing carcinogenic substances. be able to.
第１図は本発明の一実施例を示すし、酵母、乳酸菌を接
種後の材料品温の推移を示すグラフ、第２図はスィート
ツルガム搾汁残渣の水抽出液のイオンクロマトグラムで
ある。Figure 1 shows an example of the present invention, and is a graph showing changes in material temperature after inoculation with yeast and lactic acid bacteria, and Figure 2 is an ion chromatogram of an aqueous extract of sweet turgid juice residue. .
Claims (1)
型硝酸塩還元酵素を有する酵母を接種し、この酵素を利
用して硝酸態窒素を還元後、さらに菌体成分に変換し、
食・飼料等から硝酸態窒素を除去するようにしたことを
特徴とする粗飼料、園芸食品等における硝酸態窒素の除
去方法。Yeast having an assimilatory nitrate reductase is inoculated into roughage, garden foods, etc. containing nitrate nitrogen, and this enzyme is used to reduce nitrate nitrogen, which is then further converted into bacterial cell components.
A method for removing nitrate nitrogen from roughage feed, horticultural food, etc., characterized in that nitrate nitrogen is removed from food, feed, etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2338615A JP2674674B2 (en) | 1990-11-30 | 1990-11-30 | Removal method of nitrate nitrogen in roughage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2338615A JP2674674B2 (en) | 1990-11-30 | 1990-11-30 | Removal method of nitrate nitrogen in roughage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH04207160A true JPH04207160A (en) | 1992-07-29 |
| JP2674674B2 JP2674674B2 (en) | 1997-11-12 |
Family
ID=18319848
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2338615A Expired - Lifetime JP2674674B2 (en) | 1990-11-30 | 1990-11-30 | Removal method of nitrate nitrogen in roughage |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2674674B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0805205A1 (en) * | 1996-05-02 | 1997-11-05 | Societe Des Produits Nestle S.A. | Nitrate reduction system of Staphylococcus carnosus |
| JP2011010594A (en) * | 2009-07-01 | 2011-01-20 | National Agriculture & Food Research Organization | Microorganism reducing nitrate nitrogen and nitrite nitrogen in fermented feed |
| JP2018176127A (en) * | 2017-04-20 | 2018-11-15 | 有限会社オフイスヨコオ | Method for removing nitrate nitrogen and nitrate nitrogen remover |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102316396B1 (en) * | 2020-10-28 | 2021-10-26 | 한국식품연구원 | Lactobacillus plantarum WiKim0112 having nitrates-scavenging ability and composition comprising the same |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52143272A (en) * | 1976-05-24 | 1977-11-29 | Morinaga & Co | Decomposition of xanthine derivative by imcroorganism |
| JPS6043346A (en) * | 1983-07-20 | 1985-03-07 | ボブリル・リミテツド | Enzymatic removal of free amine from food |
-
1990
- 1990-11-30 JP JP2338615A patent/JP2674674B2/en not_active Expired - Lifetime
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52143272A (en) * | 1976-05-24 | 1977-11-29 | Morinaga & Co | Decomposition of xanthine derivative by imcroorganism |
| JPS6043346A (en) * | 1983-07-20 | 1985-03-07 | ボブリル・リミテツド | Enzymatic removal of free amine from food |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0805205A1 (en) * | 1996-05-02 | 1997-11-05 | Societe Des Produits Nestle S.A. | Nitrate reduction system of Staphylococcus carnosus |
| JP2011010594A (en) * | 2009-07-01 | 2011-01-20 | National Agriculture & Food Research Organization | Microorganism reducing nitrate nitrogen and nitrite nitrogen in fermented feed |
| JP2018176127A (en) * | 2017-04-20 | 2018-11-15 | 有限会社オフイスヨコオ | Method for removing nitrate nitrogen and nitrate nitrogen remover |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2674674B2 (en) | 1997-11-12 |
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