JPH04187065A - Production of anti-oxidizing substance - Google Patents

Abstract

PURPOSE:To obtain the subject substance useful as an anti-oxidizing agent in production of foods by bringing a colored waste water, etc., discharged in soysauce production processes into contact with an adsorbent, adsorbing the anti-oxidizing substance contained therein on the adsorbent and subsequently eluting the adsorbed substance using an alkalline solution. CONSTITUTION:A colored waste water discharged in soysauce production processes or soysauce is brought into contact with an adsorbent (preferably active carbon) to adsorb an anti-oxidizing substance contained in the colored waste water or soysauce on the adsorbent. The above anti-oxidizing substance adsorbed on the adsorbent is then eluted using an alkalline solution (preferably pH 10-13.5), thus obtaining the objective substance.

Description

[Detailed description of the invention] [Industrial application fields] The present invention relates to a method for producing an antioxidant substance.

[Prior Art] Conventionally, colored wastewater discharged in the soy sauce manufacturing process contains organic substances and is therefore subjected to biological treatment such as activated sludge treatment before being discharged outside the factory.

[Problem to be solved by the invention] This wastewater contains useful substances such as proteins, amino acids, and sugars, and it is possible that it could be used as a raw material for manufacturing these useful substances, but there are no reports that such an attempt has been made. There isn't.

[Means for Solving the Problem] As a result of repeated research into the effective use of colored wastewater discharged in the soy sauce manufacturing process, the present inventor discovered an antioxidant substance from the colored wastewater or soy sauce. , established an efficient separation and recovery method for this antioxidant substance, and completed the present invention.

That is, the present invention brings colored wastewater or soy sauce discharged in the soy sauce manufacturing process into contact with an adsorbent so that the antioxidants contained in the colored wastewater and soy sauce are adsorbed onto the adsorbent. The present invention relates to a method for producing an antioxidant substance, which comprises eluting the antioxidant substance with an alkaline solution.

The method of the present invention will be explained in detail below.

As a raw material for preparing the antioxidant substance, it is possible to use colored wastewater discharged from the soy sauce production process or soy sauce itself. Specific examples of colored wastewater discharged during the soy sauce manufacturing process include waste fluid generated when soy sauce is brought into contact with various chromatography resins and then recycled, the cleaning fluid for brewing tanks, and the fluid used to press soy sauce strains. Examples include cleaning liquid for filter cloths and cleaning liquid for soy sauce bottles (containers).

Further, examples of the soy sauce include soy sauce products whose expiration date has passed and pressed filtrates of poorly fermented soy sauce strains.

These raw materials may be used as they are in the method of the present invention, or may be used after being concentrated or diluted.

The adsorbent with which the above raw materials are brought into contact is not particularly limited as long as it can adsorb the antioxidant substances contained in colored wastewater or soy sauce, and activated carbon, phosphoric gel, synthetic adsorption resin, etc. can be used. can. Specifically, activated carbons include Wako Chromato Carphone (Wako Tsumugi Kogyo Co., Ltd.), Wood Ash Granules SGP, Wako CW830 B, and Wako Chromato 3.
50B (Taiko Co., Ltd.), Diahope (Mitsubishi Kasei Co., Ltd.), and other synthetic adsorption resins include decolorizing resin H3.
(Hokuetsu Tanco Co., Ltd.) are examples.

The method of contacting the raw material with the adsorbent may be either the 7<.edge method or the column method, and the column method is advantageous for continuous treatment.

The antioxidant substances adsorbed on the adsorbent are eluted from the adsorbent using an alkaline solution. The alkaline solution to be used is not particularly limited as long as it has a pH of 10 to 13.5; for example, an aqueous alkali metal hydroxide solution such as a sodium hydroxide aqueous solution or a potassium hydroxide aqueous solution having the above-mentioned liquid properties; An ammonia aqueous solution such as aqueous ammonia (ammonium hydroxide) can be used. In particular, a mixture of 1 to 40% of alcohol such as ethanol to these alkaline solutions is preferable as the eluent solution.

Elution of the antioxidant from the adsorbent can be carried out by bringing the alkaline solution into contact with the adsorbent that has adsorbed the antioxidant.
Either the hatch method or the column method can be used.

The eluate thus obtained contains an antioxidant substance, and the eluate can be used as it is as an antioxidant. Further, if necessary, the alkaline eluent may be dried to form a powder.

EXAMPLES Hereinafter, the present invention will be specifically explained with reference to Examples and Test Examples.

Example Colored wastewater collected from cleaning liquid from soy sauce brewing tanks Loll
was adjusted to pH 3.0 using 6N hydrochloric acid solution.

200 g of activated carbon was added to the waste water, and the mixture was slowly stirred and contacted for 30 minutes.

After stirring, the activated carbon obtained by filtration was added to 1. The eluate was brought into contact with 4% aqueous ammonia (pH 11,4), and the resulting eluate was concentrated to dryness, and then dried using a drier to obtain 20 g of a powdered antioxidant substance.

Test Examples The antioxidant activity of the antioxidant substances obtained in Examples was determined using rhodan iron.

Namely, ethanol (1 ml), 25% phosphoric acid solution (1 ml), 0.05M phosphorus buffer (pH 7.0)
(2+n1) and 0.005-0.02% anti-acid solution (1 ml) were mixed and incubated in the dark at 37° C. for 6 days.

Incubate 0.1ml of the reaction solution and add 9.7+nl of 75% ethanol solution, 0.1ml of 30% ammonium trinoanate solution and 0.02M
Iron chloride (FeC12) hydrochloric acid solution (3.5% ferric chloride
Add 0 and 11 (dissolved in hydrochloric acid solution) and dissolve 3 at room temperature.
After standing for a minute, absorbance at 500 nm was measured.

The results are shown in Table 1.

Table 1 *1... 2 averages of 1UII As is clear from Table 1, the antioxidant substance prepared by the method of the present invention has an antioxidant activity comparable to that of α-tocopherol.

[Effect of the invention] As shown in the above test example, the antioxidant substance obtained by the method of the invention has an antioxidant activity equivalent to that of α-tocopherol, and is extremely useful as an antioxidant during food production. It is something. Therefore, the method of the present invention, which can efficiently produce such useful substances, is extremely useful and has the excellent effect of being extremely simple to operate.

Claims (1)

[Scope of Claims] 1) Colored wastewater or soy sauce discharged in a soy sauce manufacturing process is brought into contact with an adsorbent to adsorb antioxidants contained in the colored wastewater and soy sauce to the adsorbent. A method for producing an antioxidant substance, which comprises eluting the antioxidant substance with an alkaline solution. 2) The method for producing an antioxidant substance according to claim 1, wherein the adsorbent is activated carbon. 3) The method for producing an antioxidant substance according to claim 1, wherein the alkaline solution has a pH of 10 to 13.5.