JPH0351761A - Automatic analyzer and photometric data processing - Google Patents

Abstract

PURPOSE:To determine the positions of measurements so as to match the optical characteristics of containers and to make it possible to perform highly accurate stationary measurement by stopping a plurality of the reaction containers at a plurality of the positions within one pitch from the specified position of the reaction container, and measuring the light. CONSTITUTION:A plurality of reaction containers wherein samples are contained are arranged in a reaction disk 1. The disk is turned with a pulse motor 10 of a driving mechanism 3. Luminous flux 9 is emitted. The light is measured with a photometer 7. At this time, the stopping position of the pulse motor 10 is set at the optically characteristic part of the reaction container 2, e.g. at the central part of the cylindrical container, and the measurement is performed. Then, a plurality of constant pulses are sent to a region within one pitch around the stopping position. The measurement is performed at each stopping position. The data are analyzed, and the objective position is determined. Thus the light is measured. Since the measuring position is determined based on the optical characteristics of the reaction container 2, the light can be measured highly accurately at high reproducibility.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a photometric method for an automatic analyzer, and particularly to a method for correcting variations in photometric positions.

[Conventional technology]

Conventional automatic analyzers measure light while moving the reaction vessel in order to prevent variations in photometry due to the stop position of the reaction vessel.

In addition, as seen in JP-A-63-12962, an example is shown in which beads with reagents attached to their surfaces are vibrated by a magnetic field while photometry is performed in order to reduce the influence of variations in the stopping position during photometry on the photometry. There is also.

[Problem to be solved by the invention]

The above conventional technology measures light while moving the reaction vessels and beads, so it averages over a wide range of reaction vessels and beads, which makes it easy to incorporate manufacturing variations in reaction vessels and beads, making it possible to reproduce in a high precision range. There was a problem that it was difficult to express sexuality.

An object of the present invention is to eliminate a stop error of ±1 pulse that occurs when determining a stop position by optically detecting a detection plate, perform photometry while standing still, and achieve high reproducibility.

[Means to solve the problem]

The above purpose is to first set the pulse motor stop position near an optically distinctive part of the reaction vessel, move the reaction vessel by light detection, etc., and measure the light when it stops. Send constant pulses to multiple locations, and measure light at each stop position. In this way, once multiple pieces of photometric data are obtained from areas with optical characteristics, these data are analyzed to determine the target position, and if necessary, a constant pulse is applied to the target position. Send it and perform photometry.

Note that this target position does not need to be included in the photometric positions of the plurality of data.

The optically characteristic portion of the reaction vessel is a portion whose position can be confirmed by placing a sample into the reaction vessel and photometrically measuring the sample.

For example, in the case of a cylindrical container, this would be the center of the reaction container.
At this point, the absorbance or fluorescence intensity reaches its maximum.

[Effect]

If the target photometry position is determined by analyzing the multiple pieces of data obtained as described above, the photometry position is determined by the optical characteristics of each reaction vessel itself. Dispersion of photometric data is improved.

〔Example〕

Embodiments of the present invention will be described below with reference to FIG.

Example 1 FIG. 1 shows an overview of this device.

A plurality of reaction vessels 2 are arranged on the circumference of a rotating disk-shaped reaction disk 1, and the reaction disk 1 is rotated by a drive mechanism 3. The drive mechanism 3 enables the reaction container 2 to be rotated intermittently by one pitch or to be reciprocated in short periods. The reaction disk 1 is reciprocated at high speed with minute amplitude, and the liquid in the reaction container 2 is stirred.

A reaction vessel 2 attached to the reaction disk 1 contains an acrylic-coated Permalloy stirring ball 4.

The reaction vessel 2 has an inner diameter of 6.2 m + 't'' depth of 30 I1 m.
It has a cylindrical shape, and has a smooth entrance window 5 on the bottom and a smooth exit window 6 on the side. This reaction vessel 2
is made of a translucent material such as glass or acrylic resin.

A permanent magnet or electromagnet 8 is placed near the photometric position of the photometer 7, as shown in FIG. Since the permanent magnet or electromagnet 8 attracts the stirring ball 4 in the reaction vessel 2 that has come to the photometry position to the side wall opposite to the exit window 6, the stirring ball 4 does not obstruct the path of the light beam 9.

FIG. 2 shows the spectral shape of the reaction vessel when the reaction vessel is advanced one pulse at a time.

It can be seen that the spectral shape of each reaction container 2 is uneven because the reaction container 2 is cylindrical in order to perform vibrational stirring efficiently. Therefore, even if the center of the reaction container 2 is set at the photometry stop position and the fluorescence intensity of the same concentration sample in each reaction container is measured by step feeding, the fluorescence intensity varies.

The first reason for this is that the pulse motor 10 is the driving source for transporting the reaction containers 2, and determines the stopping position of each reaction container 2 by optically detecting the detection plates that correspond to each reaction container 2 one-to-one. This is because the stop position varies by about ±1 pulse.

Figure 1 shows the shape of the fluorescence spectrum when two different successive reaction vessels were fed one pulse at a time using a pulse motor. The vertical axis is the fluorescence intensity output, and the horizontal axis is the cumulative number of pulses.

The reason why the spectrum shape is step-like is that quantization errors occur when the pulses are sent by the pulse motor.

In order to eliminate variations in measurement values due to variations in the stopping position of the above ±1 pulse,
The first stop position was set at Ax or A2 two positions before I or c2, and a total of 4 pulses were sent to the reaction vessel 2, one pulse at a time interval of 1 second. At this time, photometry was carried out for 0.5 seconds at these five stop positions, and photometry was performed near the center of the reaction vessel.

The fluorescence intensity was measured by stopping at five locations at one pulse interval.

Since the maximum value of these five fluorescence intensities was the fluorescence intensity at the center of the reaction vessel, by using this as the fluorescence intensity of the sample, variations among samples were improved.

Example 2 The second cause of variations in the fluorescence intensity measurements of samples of the same concentration for each reaction vessel 2 is due to positional errors in the attachment of the reaction vessels 2 to the reaction disk 1.

Comparing the shapes of the two spectra in FIG. 2, this installation has slightly different spectral shapes due to positional errors. For example, this is Cs-B1;6Cz-B2
This can be confirmed by .

In this installation, in order to eliminate positional errors, the average value of the maximum value of the five fluorescence intensities obtained as in Example 1 and the second largest value is calculated, and this average value is used for the sample. By setting the fluorescence intensity to , the variation among samples was improved.

Of course, among the five measured values, the one with the largest value is 3.4
．． The variation can also be improved by using each average value up to the fifth as the fluorescence intensity of the sample.

The above is an example of the CV of sample fluorescence intensity in each of the above cases.

1-point metering...1.49%...
05-point photometry maximum value adopted...0.19%...
■Using the average value of 2 data from the larger 5 data...
0.31%...■ Adopt the average value of 3 data from the largest of 5 data...0.
28%...■ Adopt the average value of 4 data from the largest of 5 data...0
．． 30%...■ Adopt the average value of the 5 data from the largest of the 5 data...0
．． 37%...■ From this, the Cv of 5-point photometry is all smaller than the Cv of 1-point photometry (Comparison of ■H■ to ■).

Among them, the Cv tends to be better when the number of data to be averaged is smaller (Comparisons from ■ to ■). In particular, when the maximum value of 5-point photometry is taken as the sample fluorescence intensity (■) gives the best CV.

〔Effect of the invention〕

According to the present invention, since the photometric position of the reaction container is determined based on the optical characteristics of the reaction container, highly accurate photometry can be performed.

[Brief explanation of drawings]

FIG. 1 is a diagram of the fluorescence spectrum shapes of two different reaction vessels, and FIG. 2 is a schematic diagram of the main parts in the example. DESCRIPTION OF SYMBOLS 1... Reaction disk, 2... Reaction container, 3... Drive mechanism. 4... Stirring ball, 5... Entrance window, 6... Output window, 7... Photometer, 8... Permanent magnet or electromagnet, 9...
...Luminous flux, diagram

Claims (1)

[Claims] 1. One or more reaction vessels containing a sample, and a turntable on which these reaction vessels are arranged on the circumference;
In an automatic analyzer equipped with a pulse motor for driving a turntable and a photometer that measures the reaction liquid in the reaction container by irradiating light onto the reaction container, each reaction container is arranged at multiple positions within one pitch. An automatic analyzer characterized by stopping and measuring light. 2. Photometry characterized by selecting the maximum or minimum value from a plurality of data for each reaction container obtained by the automatic analyzer according to claim 1, and using this as sample data. Data processing method. 3. A photometric data processing method, characterized in that among a plurality of data for each reaction container obtained by the automatic analyzer according to claim 1, some average values are taken as sample data.