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JPH03240493A - プラスミノーゲンアクチベーター遺伝子を有する融合遺伝子、およびその用途 - Google Patents

プラスミノーゲンアクチベーター遺伝子を有する融合遺伝子、およびその用途

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Publication number
JPH03240493A
JPH03240493A JP3582590A JP3582590A JPH03240493A JP H03240493 A JPH03240493 A JP H03240493A JP 3582590 A JP3582590 A JP 3582590A JP 3582590 A JP3582590 A JP 3582590A JP H03240493 A JPH03240493 A JP H03240493A
Authority
JP
Japan
Prior art keywords
gene
plasminogen activator
yeast
gene containing
fused gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP3582590A
Other languages
English (en)
Other versions
JP3041009B2 (ja
Inventor
Takashi Hiramatsu
隆司 平松
Teruhiko Beppu
別府　輝彦
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP3582590A priority Critical patent/JP3041009B2/ja
Publication of JPH03240493A publication Critical patent/JPH03240493A/ja
Application granted granted Critical
Publication of JP3041009B2 publication Critical patent/JP3041009B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。

Description

【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、Ｍｕｃｏｒ　ｐｕｓｉＬＬｕｓが産生するの
凝乳酵素であるムコールレンニン（以下、ＭＰＲという
）遺伝子とプラスミノーゲンアクチヘータ−（以下、Ｐ
Ａという）遺伝子との融合遺伝子、該融合遺伝子が挿入
されたプラスミド、該プラスミドにより形質転換された
酵母、該酵母によるＰＡの製造に関する。
さらに詳しくは、遺伝子レヘルにおいてＭＰＲのプレま
たはプレプロ配列と様々なタイプのＰＡとを連結させた
融合遺伝子を、組み換えＤＮＡの技術を応用して酵母に
て発現させることからなる様々なタイプのＰＡの効率良
い製造方法を提供する一連の技術に関する。
（従来の技術・発明が解決しようとする課題〕線維素溶
解に係わるＰＡは血栓溶解治療薬として広く用いられて
おり、その内ヒト由来のＰＡとしては、ウロキナーゼ（
以下、ＵＫという）と血（ａｌｏａ＋　　ｐ／、ｍｐ　
　１ａｐ）〔θ〕 （Ｊｏｕ＋　ｐ／ｌｕ＋ｐ　２ａｐ） 〔θ〕 巳 一見区生 継 迅　蛎 匹　く ″　　Ｓ ε −目 　　　　ａ 寸　犠　郷　− ♂

Claims (6)

    【特許請求の範囲】
  1. （１）ムコールレンニン遺伝子のプレ配列とプラスミノ
    ーゲンアクチベーター遺伝子とが連結されてなる融合遺
    伝子。
  2. （２）前記ムコールレンニン遺伝子のプレプロ領域の内
    部または下流に、前記プラスミノーゲンアクチベーター
    遺伝子が連結されてなる請求項（１）記載の融合遺伝子
    。
  3. （３）請求項（１）または（２）記載の融合遺伝子が挿
    入されてなるプラスミド。
  4. （４）プロモーターとして酵母ＧＡＬ７プロモーターを
    有する請求項（３）記載のプラスミド。
  5. （５）請求項（３）または（４）記載のプラスミドによ
    り形質転換された酵母。
  6. （６）請求項（５）記載の酵母を発現させることを特徴
    とするプラスミノーゲンアクチベーターの製造方法。
JP3582590A 1990-02-15 1990-02-15 プラスミノーゲンアクチベーター遺伝子を有する融合遺伝子、およびその用途 Expired - Fee Related JP3041009B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP3582590A JP3041009B2 (ja) 1990-02-15 1990-02-15 プラスミノーゲンアクチベーター遺伝子を有する融合遺伝子、およびその用途

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP3582590A JP3041009B2 (ja) 1990-02-15 1990-02-15 プラスミノーゲンアクチベーター遺伝子を有する融合遺伝子、およびその用途

Publications (2)

Publication Number Publication Date
JPH03240493A true JPH03240493A (ja) 1991-10-25
JP3041009B2 JP3041009B2 (ja) 2000-05-15

Family

ID=12452732

Family Applications (1)

Application Number Title Priority Date Filing Date
JP3582590A Expired - Fee Related JP3041009B2 (ja) 1990-02-15 1990-02-15 プラスミノーゲンアクチベーター遺伝子を有する融合遺伝子、およびその用途

Country Status (1)

Country Link
JP (1) JP3041009B2 (ja)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2012323419B2 (en) 2011-10-13 2016-02-04 Dow Global Technologies Llc Biocidal compositions and methods of use

Also Published As

Publication number Publication date
JP3041009B2 (ja) 2000-05-15

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