JPH03107747A - Container for detecting electrochemical emission - Google Patents

Abstract

PURPOSE:To improve a detection sensitivity by spirally rolling an insulation sheet wherein a working electrode is formed on one surface and a counter electrode is formed on another surface, closing the tip end and also fixing a light transmission plate on one side surface of the insulation sheet. CONSTITUTION:A spiral state of the insulation sheet 11, i.e. a space between the working electrode WE and the counter electrode CE is maintained by the light transmissive plate 12 and a light reflective plate 13. The subject container functions as a liquid storing container by the plate 13 and an insulation spacer 14. An emission generated near the electrode WE is partially reflected by the plate 13 and attains to a light detecting device passing through the plate 12 in the solution to be tested. Further, the plate 12 and the light detecting device can get extremely near since no obstacle exists at the side of plate 12. On the other hand, the areas of electrodes WE, CE become large by arranging these electrodes spiral and the emitted light quantity is increased. Consequently, the received light quantity on the light receiving surface of photodetector is increased, thereby the detection sensitivity is improved.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to an electrochemiluminescence detection container that increases the amount of luminescence and improves detection sensitivity.

[Conventional technology]

Detection used in electrochemiluminescence detection, such as quantitative measurement of various antibodies by measuring the amount of light emitted at that time, by making use of the phenomenon in which the light emission of a luminescent substance immobilized on an antigen is suppressed in an antigen-antibody reaction. There is a container 1 for use as shown in FIG. That is, the working electrode WE, which is a plane electrode and has a reflective mirror surface, is formed on the inner surface of the bottom wall 3a of the cylindrical container 3 containing the test solution 2 containing a luminescent substance. At a portion of the inner circumferential surface 3b of the cylindrical container 3 facing each other, with a certain distance G from the working electrode WE,
It is formed by forming a counter electrode CE which is a substantially ring-shaped surface electrode having a slit portion S in a part thereof and whose surface is a reflective mirror surface. The working electrode WE is formed with an elongated lead portion WE-1 that passes through the slit portion S of the counter electrode CE and reaches the upper edge 3c of the cylindrical container 3.
An elongated lead portion CE-1 extending to the upper edge 3c of the cylindrical container 3 is formed parallel to the lead portion WE-1 on the working electrode WE side.

To detect electrochemiluminescence using the detection container 1,
A pulse voltage generator 5 (for example, composed of a function generator and a potentiostat) is connected to the lead portions WE-1 and CE-1 via a connecting means 4 such as a clip, and a detection container is connected to the lead portions WE-1 and CE-1. Directly above the upper opening of the detection container 1, a photodetection device 6 (for example, a photodetector such as a photomultiplier and a preamplifier) is installed that receives light from inside the detection container 1 and displays the strength of the received light amount in a graph or numerical form. , a pulse counter, and a signal output device such as an oscilloscope).

Then, the test solution 2 is put into the detection container 1 until the liquid level exceeds the counter electrode CE, and the pulse voltage generator 5 is operated to apply a predetermined pulse voltage between the working electrode WE and the counter electrode CE. Apply. Test solution 2 is removed by applying a pulse voltage.
The luminescent substance contained therein emits light, and the amount of light emitted is converted into a predetermined unit by the photodetector 6 and displayed as a graph or number.

The test solution 2 used in the electrochemiluminescence detection container 1 includes an aqueous solution containing a buffer solution in which an antigen-antibody reaction is performed, and the antigen includes various proteins, polypeptides, and polysaccharides. , lipids, nucleic acids, etc., and examples of luminescent substances that can be used include luminol, pyrene, anthracene, rubrene, and their 818 forms. Furthermore, the luminescent substance is immobilized on the antigen using a common crosslinking agent.

[Problem to be solved by the invention]

In this prior art, the source of light emitted near the working electrode WE is a pair of working electrode WE, counter electrode CE,
Moreover, its publication area is small. Therefore, the photodetector 6
The amount of light received (illuminance) on the light-receiving surface of is small because the amount of light emitted (luminous intensity) of the light-emitting substance that is the light source is small.Therefore, this conventional technology has the problem that detection sensitivity is not sufficient.

This invention was made with the aim of solving the problems of the prior art.

[Means to solve the problem]

Means for solving the above problems will be explained below using FIG. 1 corresponding to an embodiment. In this invention, an insulating sheet 11 having a working electrode WE formed on one side and a counter electrode CE formed on the other side is wound spirally, and its tip is closed. A light reflecting flat plate 13 is fixed to one side of the insulating sheet 11 and a sample injection plug 15 is screwed thereto, and a light reflecting plate 13 is fixed to the other side of the insulating sheet 11.

[For production]

In this structure, the light emitting source includes a plurality of working electrodes WE and counter electrodes CE, and the light emitting area thereof is large.

〔Example] FIG. 1 is a diagram showing an embodiment of the present invention.

In Fig. 1, 10 is a detection container, 11 is an insulating sheet, WE is a working electrode, CE is a counter electrode, 12 is a light-transmitting flat plate, 13 is a light-reflecting flat plate, 4 is an insulating spacer, and 15 is a sample. It is an injection plug. The insulating sheet 11 is made of epoxy, acrylic, styrene resin, etc., and has a width of, for example, 10 mm. OX thickness 0゜5 x length 40.0
It is the torso.

The working electrode WE is a vapor-deposited thick film, sputtered film, printed film, or plate of a conductive material, preferably a metal with a high redox potential such as platinum, gold, or silver, formed on one surface of the insulating sheet 11. , its thickness is 1-100 pm.

The counter electrode CE is formed on the other surface of the insulating sheet 11.
It is a vapor-deposited thick film, sputtered film, printed film, or plate of a conductive material, preferably a metal with a high redox potential such as platinum, gold, or silver, and has a thickness of 1 to 100 μm.

The insulating sheet 11 has the working electrode WE formed on one side and the counter electrode CE formed on the other side, for example, with the counter electrode CB on the inside,
With the working electrode WE on the outside, the working electrode WE and the counter electrode CE
It is wound spirally so that the gap between the two ends is approximately 0.1 to 2.0 m, and the tip thereof is closed with an insulating spacer 14 interposed therebetween. A lead portion WE-1 to which a connecting means such as a clip is connected is formed in a part of the working electrode WE.

Further, a lead portion CE-1 to which a connecting means such as a clip is connected is formed in a part of the counter electrode CB. The working electrode WE and the counter electrode CE are planar electrodes with reflective mirror surfaces.

The light-transmitting flat plate 12 is made of glass, for example, and is fixed to one side of the insulating sheet 11.

The tip of the insulating spacer 14 on the light-transmitting flat plate 12 side is notched, and the notch 16 serves as an air vent.

The light reflecting flat plate 13 is, for example, a back mirror made of acrylic resin or glass, or various front mirrors, and is fixed to the other side of the insulating sheet 11 . The light reflecting plate 13 is provided with a threaded hole 13A into which the sample injection plug 15 is screwed.

The sample injection plug 15 is removed and a sample is supplied from the screw hole 13A.

The light-transmitting flat plate 12 and the light-reflecting flat plate 13 maintain the spiral state of the insulating sheet 11, in other words, the gap between the working electrode WE and the counter electrode CE. The light reflecting flat plate 13 and the insulating spacer 14 function as a liquid storage container.

A portion of the light emitted near the working electrode WE is reflected by the light-reflecting flat plate 13, and the light-transmitting flat plate 12 is reflected in the test solution.
to reach a photodetector (not shown). Since there are no obstacles on the side of the light-transmitting flat plate 12, the light-transmitting flat plate 12 and the photodetecting device can be very close to each other.

By arranging the working electrode WE and the counter electrode CE in a spiral, their area becomes larger, and therefore the amount of light emitted increases. Therefore, the amount of light (illuminance) received at the light-receiving surface of the photodetector increases, and its detection sensitivity improves.

Although the insulating sheet 11 is spirally wound into a circular shape, it is not limited to this, and may be rectangular or the like. That is, the term "helical" refers to the fact that the working electrode WE and the counter electrode CE are wound in multiple layers with a constant gap between them. Also, instead of the insulating spacer 4, the insulating sheet 1
The electrode may not be formed inside the tip of -11, but that portion may be fixed and the tip may be closed.

〔Effect of the invention〕

As explained above, this invention consists of an insulating sheet having a working electrode formed on one side and a counter electrode formed on the other side, wound in a spiral shape, the tip of which is closed, and a light-transmitting flat plate. A light reflecting flat plate fixed to one side of the insulating sheet and having a sample injection plug screwed thereto, and a light reflecting flat plate having a sample injection port formed on the other side of the insulating sheet fixed. be. Therefore, the light emitting source has a large light emitting area. Therefore, according to the present invention, it is possible to obtain the effect that detection sensitivity can be improved.

[Brief explanation of drawings]

FIG. 1 is a diagram showing an embodiment of the present invention, in which (a) is an exploded perspective view, (b) is a sectional view, and FIG. 2 is a diagram showing a conventional technique; (a) is a sectional view; b) is a perspective schematic view. 11... Insulating sheet, WE... Working electrode, CE.
... Counter electrode, work 2... Light-transmitting flat plate, 13... Light-reflecting flat plate, 14... Insulating spacer

Claims (1)

[Claims] 1. An insulating sheet having a working electrode on one side and a counter electrode on the other side is wound in a spiral shape, the tip of which is closed, and a light-transmitting flat plate is fixed to one side of the insulating sheet. , an electrochemiluminescence detection container in which a light reflecting plate to which a sample injection stopper is screwed is fixed to the other side of the insulating sheet;