JPH0265773A - Enzymatic thremistor apparatus - Google Patents
Enzymatic thremistor apparatusInfo
- Publication number
- JPH0265773A JPH0265773A JP21362088A JP21362088A JPH0265773A JP H0265773 A JPH0265773 A JP H0265773A JP 21362088 A JP21362088 A JP 21362088A JP 21362088 A JP21362088 A JP 21362088A JP H0265773 A JPH0265773 A JP H0265773A
- Authority
- JP
- Japan
- Prior art keywords
- carrier liquid
- temperature
- thermistor
- column
- enzymatic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000002255 enzymatic effect Effects 0.000 title abstract 3
- 239000007788 liquid Substances 0.000 claims abstract description 34
- 238000010438 heat treatment Methods 0.000 claims abstract 3
- 108090000790 Enzymes Proteins 0.000 claims description 13
- 102000004190 Enzymes Human genes 0.000 claims description 13
- 108010093096 Immobilized Enzymes Proteins 0.000 abstract description 2
- 238000006911 enzymatic reaction Methods 0.000 abstract 2
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 238000005259 measurement Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、臨床化学分析装置、食品工業、発酵工業、医
薬品工業、化学工業等の工業プロセス計ａｌ、環境計計
１装置として用いられるフローインジエンヨン分析系の
酵素サーミスタ装置に関するしのである。Detailed Description of the Invention [Industrial Application Field] The present invention is a flow analyzer used as a clinical chemistry analyzer, an industrial process meter, an environmental meter, etc. in the food industry, fermentation industry, pharmaceutical industry, chemical industry, etc. This article is about an enzyme thermistor device for an analysis system.
従来の上記酵素サーミスタ装置は第３図に示すようにな
っており、図中１はキャリア液２を入れるキャリア液槽
、３は先端にフィルタ４をつけてキャリア槽１内に入れ
た送液チューブ、５はペリスタポンプ、６はダンパ、７
はインジェクションバルブであり、これらは上記送液チ
ューブ３に直列状に配設されている。８はインジェクシ
ョンバルブ７に試料を注入するインジェクタ、９は恒温
槽で、この恒温槽９内に、上記送液チューブ３のインジ
ェクションバルブ７より下流側にコイル状に構成した熱
交換部］０と、送液チューブ３の下流端に接続され、か
つ固定化生体素子（固定化酵素）１１を充填したカラム
１２と、カラム１２内の固定化生体素子１１の発熱量を
検知するサーミスタ１３とが配置されている。１４はサ
ーミスタ１３にリード線１５にて接続したブリッジ増幅
回路、１６はレコーダである。また１７は上記カラム１
２からの排液１８を溜める排液溜めである。The conventional enzyme thermistor device is shown in Fig. 3, in which 1 is a carrier liquid tank into which a carrier liquid 2 is placed, and 3 is a liquid feeding tube with a filter 4 attached to the tip and inserted into the carrier tank 1. , 5 is a peristaltic pump, 6 is a damper, 7
are injection valves, which are arranged in series with the liquid feeding tube 3. 8 is an injector for injecting the sample into the injection valve 7; 9 is a constant temperature bath; within this constant temperature bath 9, a heat exchange section configured in a coil shape on the downstream side of the injection valve 7 of the liquid feeding tube 3; A column 12 connected to the downstream end of the liquid feeding tube 3 and filled with an immobilized biological element (immobilized enzyme) 11, and a thermistor 13 for detecting the calorific value of the immobilized biological element 11 in the column 12 are arranged. ing. 14 is a bridge amplifier circuit connected to the thermistor 13 by a lead wire 15, and 16 is a recorder. Also, 17 is column 1 above.
This is a drain reservoir that collects the drain fluid 18 from 2.
」、１：己（１η成１こおいて、インジエクンヨンバル
ブ７にインジェクタ８にて注入された試料はギヤ１ア液
２にて送られ、恒温槽９内の熱交換部ＩＯにて所定の温
度に調’ｉＨされてからカラム１２に流入し、ここで固
定化生体素子１１に接触し、その後排出される。カラム
１２内に流入した試料は固定化生体素子］１に接触して
分解され、固定化生体素子１１はこの分解反応によって
発熱あるいは吸熱して熱変化が生じ、この熱変化かサー
ミスタ１３にて検知され、ブリッジ増幅回路１４を経て
レコーダ１６に記録される。'', 1: self (1η) At this point, the sample injected into the injection valve 7 by the injector 8 is sent by the gear 1a and the liquid 2, and is heated to a predetermined temperature by the heat exchange part IO in the thermostatic chamber 9. The sample flows into the column 12, where it comes into contact with the immobilized biodevice 11, and is then discharged.The sample that flows into the column 12 comes into contact with the immobilized biodevice 1 and decomposes. The immobilized biological element 11 generates heat or absorbs heat due to this decomposition reaction, causing a thermal change. This thermal change is detected by the thermistor 13 and recorded on the recorder 16 via the bridge amplification circuit 14.
上記恒！ＦＡ ＲＱ内の温度は化学反応速度及び酵素活
性、酵素寿命との関係から３０℃程度に設定されている
、このため、外気温が低い場合、キャリア液２と恒１２
ｉ槽９内の温度差が大きく熱交換が不充分となり、その
結果として計測が不正確なものとなり、キャリア液２の
流速を速くしたときにその傾向は更に大きくなる。Kou above! The temperature inside the FA RQ is set at around 30℃ due to the relationship between chemical reaction rate, enzyme activity, and enzyme life. Therefore, when the outside temperature is low, carrier liquid 2 and constant temperature 12
The temperature difference within the i-tank 9 is large, resulting in insufficient heat exchange, resulting in inaccurate measurements, and this tendency becomes even more pronounced when the flow rate of the carrier liquid 2 is increased.
本発明は上記のことにかんがみなされたらので、外気、
Ｒが低い場合でも、その影ｊ５が少なく正確な計７則が
でき、また恒温槽内での計ｌｌｌ’ｌ試料の温度が所定
の温度に達するまでの時間が短くなり、計１１ｐｔ速度
の向上を図ることができ、さらに熱交換部を小さくして
恒温槽の小型化を図ることができ、そしてさらにキャリ
ア液の流速を速くしても恒ｆＡ槽内での熱交換の安定性
、容易さに与える外乱が極めて少なくなり、安定した＝
１側を能率よく行なうことができる酵素サーミスタ装置
をｔ２倶することを目的とするものである。Considering the above, the present invention provides outside air,
Even when R is low, the influence j5 is small and a total of 7 accurate rules can be made, and the time it takes for the total sample temperature to reach the predetermined temperature in the thermostatic chamber is shortened, increasing the speed by a total of 11 points. Furthermore, the heat exchange part can be made smaller to make the constant temperature chamber smaller, and even if the flow rate of the carrier liquid is increased, the stability and ease of heat exchange within the constant fA chamber can be improved. Stable = extremely little disturbance applied to
The purpose of this invention is to provide an enzyme thermistor device that can efficiently operate the first side.
上記目的を達成するために、本発明に係る酵素サーミス
タ装置は、計；ＩＰＩ Ｌようとする試す−１をキャリ
ア液ｔａからのキャリア液と共に恒ＩＨｆｅ内に設けた
熱交換部を介して固定化生体素子を充填したカラム内に
流し、このカラム内の固定化生体素子の熱変化をサーミ
スタにて検知するようにした酵素サーミスタ装置におい
て、上Ｊ己キャリア液漕内に、キャリア液を所定の、３
度にＩＪＩＩ温するヒータを内装した構成となっている
。In order to achieve the above object, the enzyme thermistor device according to the present invention immobilizes the total IPI L sample-1 together with the carrier liquid from the carrier liquid ta through a heat exchange section provided in the constant IHfe. In an enzyme thermistor device in which a biological element is flowed into a column packed with the biological element and a thermistor is used to detect a thermal change of the immobilized biological element in the column, a carrier liquid is placed in a carrier liquid tank at a predetermined level. 3
It has a built-in heater that heats up to about 100 degrees.
キャリアｉ＆ Ｆｆｌ内のキャリア液はヒータにて加１
Ｂされ、外気温に関係なく、恒温槽内の温度に近い温度
で恒温槽内の熱交換部に流入される。The carrier liquid in Carrier i & Ffl is added with a heater.
B, and flows into the heat exchange section in the thermostatic oven at a temperature close to the temperature inside the thermostatic oven, regardless of the outside temperature.
本発明の実施例を第１図に基づいて説明する。 An embodiment of the present invention will be described based on FIG.
なおこの実施例において第３図に示す従来例のものと同
一部祠は同一符号を付して説明を省略する。In this embodiment, parts of the shrine that are the same as those of the conventional example shown in FIG. 3 are given the same reference numerals, and their explanation will be omitted.
図中２０はキャリア液２を入れるキャリア液槽てあり、
これにフィルタ４をつけた送液チューブ３の先端部が入
れられている。このキャリア液槽２０は蓋２１にて閉じ
られるようになっていて、このキャリア液槽２０内には
ヒータ２２と、サーミスタ等の感温素子２３が入れてあ
り、これらはリード線２４．２５を介して温度制御装置
２６に接続されている。In the figure, 20 is a carrier liquid tank into which carrier liquid 2 is placed.
The tip of the liquid feeding tube 3 with the filter 4 attached thereto is inserted. This carrier liquid tank 20 is designed to be closed with a lid 21, and a heater 22 and a temperature sensing element 23 such as a thermistor are placed inside this carrier liquid tank 20, and these are connected to lead wires 24 and 25. The temperature control device 26 is connected to the temperature control device 26 via the temperature control device 26 .
上記構成において、ヒータ２２に通電することによりキ
ャリア液槽２０内のキャリア液２が加温され、このとき
の温度は感温素子２３にて検出され、温度制ｉ＠Ｉ装置
２６にて制御される。In the above configuration, the carrier liquid 2 in the carrier liquid tank 20 is heated by energizing the heater 22, and the temperature at this time is detected by the temperature sensing element 23 and controlled by the temperature control i@I device 26. Ru.
キャリア液２は恒温槽９内の温度に比べてあまり低くな
い温度になるように制御される。The temperature of the carrier liquid 2 is controlled to be not much lower than the temperature inside the constant temperature bath 9.
酵素サーミスタ装置の作動峙においてキャリア液２は上
記したように恒温槽９内の１品度よりやや低い温度に加
温されているため、外気ｌＨと恒温槽９内温度に大きな
差があっても恒ｉＡＬ ｔ６９内での熱交換が短時間に
行なわれる。During the operation of the enzyme thermistor device, the carrier liquid 2 is heated to a temperature slightly lower than the temperature in the thermostatic chamber 9 as described above, so even if there is a large difference between the outside air lH and the temperature inside the thermostatic chamber 9. Heat exchange within the constant iAL t69 takes place in a short time.
第２図は上記酵素サーミスタ装置を携帯用にまとめた状
態を示すもので、上記各構成部材は携帯ケース２７に収
納されている。FIG. 2 shows the enzyme thermistor device in a portable state, with each of the components described above being housed in a carrying case 27.
本発明によれば、外気温が低い場合でも、その影響が少
なく正確な計測ができる。また恒温槽９内での計ａｌｌ
試料の温度が所定の温度に達するまでの時間が短くなり
、計Ｕ＋速度の向上を図ることができる。さらに熱交換
部を小さくして恒ｌＲｈ５９の小型化を図ることができ
る。そしてさらにキャリア液２の流速を速くしても恒１
’Ｒｔ’Ｑ９内での熱交換の安定性、容易さに与える外
乱が極めて少なくなり、安定した計４Ｐ１を能率よく行
なうことかできる。According to the present invention, even when the outside temperature is low, accurate measurement can be performed with less influence. In addition, all measurements in the constant temperature bath 9
The time required for the temperature of the sample to reach a predetermined temperature is shortened, and the total U+ speed can be improved. Furthermore, by making the heat exchange portion smaller, the size of the constant Rh 59 can be reduced. Even if the flow rate of carrier liquid 2 is further increased, the
Disturbances affecting the stability and ease of heat exchange within 'Rt' Q9 are extremely reduced, and a total of 4P1 can be performed stably and efficiently.
第１図は本発明の要部の実施例を示す斜視図、第２図は
携帯用の酵素サーミスタ装置を示す斜視図、第３図は従
来の酵素サーミスタ装置の概略的な構成説明図である。
２はキャリア液、９は恒温槽、１０は熱交換部、１１は
固定化生体素子、１２はカラム、２０はキャリア液槽、
２２はヒータ。
出願人 株式会社 小 松 製 作 所代理人 弁
理士 米 原 正 章FIG. 1 is a perspective view showing an embodiment of the main part of the present invention, FIG. 2 is a perspective view showing a portable enzyme thermistor device, and FIG. 3 is a schematic structural explanatory diagram of a conventional enzyme thermistor device. . 2 is a carrier liquid, 9 is a constant temperature bath, 10 is a heat exchange section, 11 is an immobilized biological element, 12 is a column, 20 is a carrier liquid tank,
22 is a heater. Applicant Komatsu Manufacturing Co., Ltd. Representative Patent Attorney Masaaki Yonehara
Claims (1)
ア液２と共に恒温槽９内に設けた熱交換部１０を介して
固定化生体素子１１を充填したカラム１２内に流し、こ
のカラム１２内の固定化生体素子１１の熱変化をサーミ
スタ１３にて検知するようにした酵素サーミスタ装置に
おいて、上記キャリア液槽２０内に、キャリア液２を所
定の温度に加温するヒータ２２を内装したことを特徴と
する酵素サーミスタ装置。The sample to be measured is flowed together with the carrier liquid 2 from the carrier liquid tank 20 into a column 12 filled with immobilized biological elements 11 through a heat exchanger 10 provided in a constant temperature bath 9, and the immobilization within this column 12 is carried out. The enzyme thermistor device is configured to detect thermal changes in the biochemical element 11 using a thermistor 13, characterized in that a heater 22 for heating the carrier liquid 2 to a predetermined temperature is installed in the carrier liquid tank 20. Enzyme thermistor device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21362088A JPH0265773A (en) | 1988-08-30 | 1988-08-30 | Enzymatic thremistor apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21362088A JPH0265773A (en) | 1988-08-30 | 1988-08-30 | Enzymatic thremistor apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0265773A true JPH0265773A (en) | 1990-03-06 |
Family
ID=16642186
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP21362088A Pending JPH0265773A (en) | 1988-08-30 | 1988-08-30 | Enzymatic thremistor apparatus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0265773A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5138650A (en) * | 1990-09-27 | 1992-08-11 | Motorola, Inc. | Cordless telephone with internal debit and credit memory |
-
1988
- 1988-08-30 JP JP21362088A patent/JPH0265773A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5138650A (en) * | 1990-09-27 | 1992-08-11 | Motorola, Inc. | Cordless telephone with internal debit and credit memory |
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