JPH02223828A - Ultraviolet spectrophotometer - Google Patents

Abstract

PURPOSE:To allow the permanent preservation of measurement data and the miniaturization of the device by executing detection by using an element dyed with a melanine dye for a detecting element. CONSTITUTION:The light from a light source part 1 is reflected by plane mirrors 2, 4 and arrives at a grating 5 from which the light is made into monochromatic light and is made incident to a measuring cell 6. The transmitted light from the cell 6 enters a detecting element part 7. The transmitted light arriving at the detecting element part 7 is absorbed in the detecting element according to the intensity of the transmission. The detecting element is obtd. by dyeing the entire surface of an agarose gel with the melanine dye. The ratio of the absorption of the detecting element absorbing UV is read by an optical card reader and is expressed in terms of a concn. The melanine dye which absorbs the UV light is different in the surface condition from the melanine dye which does not absorb the UV light and, therefore, the concn. is determined from the surface areas of the different surfaces.

Description

[Detailed description of the invention] (b) Industrial application field The present invention relates to an ultraviolet spectrometer.

(b) Prior art For example, a conventional ultraviolet spectrophotometer has the configuration shown in Figure 2. This is done by separating the bright white elements emitted from a light source using a spectrometer, and applying the nine monochromatic elements to a sample solution (for solutions that do not exhibit the original absorption, a coloring reagent is added to cause a chemical reaction to develop a color). The target component in the sample solution is analyzed by photoelectric photometry of nine luminous fluxes passing through the sample solution.

Strength of incident source 1. When a monochromatic light beam passes through a liquid layer of concentration C1 and length 4, the original is absorbed and its intensity decreases. If the intensity of the luminous flux immediately after passing through the light beam is ``t-1t'', then the following relationship holds between It and 10, which is called the Ranhart-Beer law.

gcj It=Io・10 ・・・・・・・・・φ・Yu…・
(1) Here, the coefficient is a constant determined by the type of molecular compound and the wavelength, and is called the extinction coefficient, and the sample concentration C = IM.

It is called the IIt molar extinction coefficient of e when 1=1 cm.

1t and 10 (In relation to D, l t / 1 o == t
f transmittance, transmittance expressed as a percentage tX100=Tt
- Transmission Expressed by Barsen's law, person = e-c-1... (2) and the absorbance is proportional to the concentration of the sample solution.

No. 0 Problems to be Solved by the Invention ■ Conventionally, the measuring section (light source to absorption center/I/) and the detection section <1gt output device to indicator) were integrated, so the entire device was large-scale. It became something.

■With conventional equipment, the only way to save measurement data was to print out the output from the detector and save it.

■The detector uses an expensive 7-diode array, making the device expensive and expensive.

- Only the photometering section or the detection section broke down, making it difficult to repair because the reading device was integrated.

B) Elaborate means for solving the problem The present invention is characterized in that detection is performed using elemental consonants dyed with melanin pigment as a detection element.

The element used here is formed by staining with melanin on a substrate such as agarose gel.

Melanin pigment is the cause of sunburn and absorbs ultraviolet light well, and in the present invention, the ultraviolet light corresponds to the transmitted light from the measurement cell. That is, in the present invention, a measurement cell containing a sample is first irradiated with ultraviolet light, and the transmitted light from the cell is absorbed by the melanin pigment.

The rate of change in the absorption of ultraviolet light by melanin pigment varies depending on the intensity of light irradiation. Therefore, the degree of absorption of ultraviolet light changes depending on the intensity of the transmitted light from the measurement unit, that is, the concentration value of the sample component.

Therefore, by reading the violet light absorption percentage of melanin pigment, it can be converted into the concentration value of the sample component.

Specifically, the successive increase in the absorption rate of ultraviolet light from melanin pigments can be confirmed by using a commercially available source that irradiates an optical recording medium (original card) with the source, reads the changes in reflectance and transmittance of the recording area, and converts it into an electrical signal. Card reader available.

(e) Function The element dyed with melanin pigment detects and records light carrying analytical information, so the photometric section and the detection section can be separated both temporally and functionally by this element.

(f) Example An example of the present invention is shown in FIG. 1 is a light source section which houses a deuterium rung and a tungsten rung. 2,3.
4 is a plane and a spherical mirror, 5 is a grating, and 6 is a measurement cell.

jt from the light source section 1 is reflected at 2.4, reaches the grating 5, becomes a monochromatic source, and enters the measurement center/I/6.

The transmitted light from the measurement cell 1v6 enters the detection element section 7.

The configuration of the detection element section 7 is shown in FIG.

Reference numeral 7a denotes a detection element holder made of a transparent member, and the holder is held by a movable frame 7b and can be moved vertically and horizontally. By moving, the incident point of transmitted light changes and information can be recorded at different points.

Movement is performed by connecting a pulse motor (not shown) and a binion 7C. Note that 7d is a fixed frame, and 7e is a holder drawer.

When inserting the detection element 8'fr into the holder 7a, it is sufficient to hold it in the holder drawer part 7e' and pull it toward you as shown in the figure. The detection element 8 is formed by staining the entire surface of a rectangular agarose gel with melanin.

The transmitted light that has reached the detection element section 7 is collected by the detection element 7 according to the intensity of the transmitted light (that is, the m degree of the sample component).

The detection element 8 that has absorbed the ultraviolet light reads the absorption rate using a commercially available original card reading device. Convert to ffl. In addition, in order to convert into a concentration value, a calibration curve is created in advance using a standard sample with a known concentration.

After completing one measurement, move the entire detection element holder to change the incident point of the transmitted light, and obtain multiple measured values (the same sample or different samples) with one ntf detection element. Get.

Also, ultraviolet light? Since the absorbed melanin pigment has a different surface condition than the unabsorbed melanin pigment, the concentration can be determined from the surface area of the different surfaces.

(g) Effects Since the present invention uses an element dyed with melanin as a detection element, if the element is stored forever, the measurement data is stored forever.

! ! Additionally, the analysis section and detection section can be completely separated, making it possible to downsize the device with the analysis section as its main component.

[Brief explanation of the drawing]

W! Figure IJ1 is the second overall diagram of the original measuring device according to the present invention.
The figure is a schematic diagram of the configuration of a conventional device, and FIG. 3 is a detailed diagram of the original card section. 1... Light source part 5... Grating 6...
Measuring cell/L/7...Detection element section Fig. 3

Claims (1)

[Claims] 1. An ultraviolet spectrophotometer whose detection element is an element dyed with melanin pigment.