JPH02174790A - Production of sennoside - Google Patents

Abstract

PURPOSE:To produce sennoside useful as a cathartic all the year round at a low cost by carrying out hydroponic culture of Cassia angustifolia. CONSTITUTION:Cassia angustifolia is cultured by hydroponic culture and the obtained plant is extracted to obtain sennoside.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a method for cultivating senna and a method for producing sennoside, which is a diathurone glycoside.

Senna and the sennosides contained in senna are
It is known to have a laxative effect and has been used worldwide as a laxative since ancient times.

[Conventional technology]

A plant belonging to the Fabaceae family, senna (chinneberry senna Ti)
nasvel17 se+ua, Ca5sis *a
1wstHolis Vibl, or Alexandriasenna Alffixsmdriam s@ans,
Ca5si*xc++Lifoli* Dslile,
Plants belonging to the genus Ca5sis and containing sennosides, such as Ca5sis, contain sennosides in their above-ground and underground parts. The leaflets and fruits are especially rich in sennosides, so you can take them as they are dried or use sennoside extract 1.
fee.

Senna does not grow naturally in Japan, so Japan relies entirely on imports from India, Africa, etc., but its quality and supply are unstable. Cultivating senna domestically has been considered in the past, but it has never been carried out on a commercial scale because water management is difficult, it is susceptible to pests and diseases, and it often dies.

Therefore, it has been considered to cultivate senna and Rbeam genus plants (so-called Oga; also containing sennosides) by a plant tissue culture method and collect sennosides from the culture. However, in either case, the sennoside content is only about 0.01% at most, which is less than 1/100 of that of the natural mother plant, so this has not been put into practice.

[Problem that the invention seeks to solve]

In view of the above-mentioned current situation, an object of the present invention is to provide a method for efficiently obtaining senna grass and sennosides without relying on natural imported senna, which is in short supply.

[Means for solving problems]

The present invention relates to a method for cultivating senna as a sennoside raw material or a laxative/senna to be taken as it is, which is characterized by cultivating senna hydrolytically, and a method for cultivating senna hydrolytically and extracting sennoside from the obtained senna. The present invention provides a method for producing sennosides.

As is well known, hydroponic cultivation is a cultivation method that does not use normal soil at all. Depending on the type of medium, culture solution, and air supply method, hydroponic cultivation, aeroponic cultivation (spray cultivation, etc.), fixed medium cultivation, etc. There are various cultivation methods (sand cultivation, charcoal cultivation, rock wool cultivation, etc.), but senna, which is difficult to grow on the ground, grows well with hydroponic cultivation regardless of the method. In addition, there is no significant difference in the sennoside content of the grass, regardless of the method used.
Moreover, since results comparable to natural senna can be obtained, any method can be adopted depending on the situation.

A feature of hydroponic cultivation is that it is easy to artificially maintain favorable cultivation conditions, so if senna is hydroponically cultivated and the resulting senna plant is used as raw material, sennosides can be supplied more stably than before. It becomes possible. In addition, it has been confirmed that hydroponically cultivated senna flowers and bears fruit in about 2 to 3 months, and can bloom all year round, making it possible to cultivate senna through complete hydroponics, including seed collection and breeding. be.

Note that the senna to be hydroponically cultivated is not limited at all, and any species can be used.

For the culture solution for hydroponic cultivation, use a well-known synthetic nutrient medium devised for hydroponic cultivation of various plants, that is, one containing inorganic salts, organic components, vitamins, amino acids, growth regulators, etc. However, any other substance can be included as long as it is effective in accumulating sennosides. A typical example of a specific culture medium composition is the so-called garden trial formulation balanced culture solution [Ct(Now)i・411
20:11.55t/i, KNOs:01Ill/L
MtSO, -7HtO: 0.5t/1°NBaHzP
Oa: O, 155g/a, N5Fe-EDTA: 20
mt/fi, 8380. :3mg/II, MmSO,
・41110: 2 Hou 1/112nSO4・7H!
O: 0.22mt/1, C++SO,・5H,0
:0.05+B/l, Ha, Mob, ・2H, O: 0
．． 02 t/11, too-41100 times diluted solution of fine powder Hyponex (manufactured by Hyponex, 6.5% nitrogen, 6.0% soluble phosphoric acid, 11.0% water-soluble potassium), but other commercially available Any of the hydroponic fertilizers listed above can also be used.

The pH of the culture solution is approximately 5.0-10.0, and the culture temperature is approximately 5-10.0.
It is desirable to control the temperature within the range of 50°C. During cultivation, renewal of the culture solution, control of illuminance, etc. may be carried out according to conventional methods for hydroponic cultivation, but slightly higher illuminance gives preferable results.

Hydroponically cultivated senna contains sennosides in both the above-ground and underground parts, so all of them can be used as sennoside raw materials, but the sennoside content in the underground part is slightly lower than that in the above-ground part. .

No special method is required to extract sennosides from the harvested senna plant; just like when extracting from natural senna, sennosides can be extracted from whole or ground dried ground parts using water, hydrophilic organic solvents, or mixtures thereof. You can extract it using .

〔Example〕

The present invention will be explained below with reference to Examples. The sennoside content values shown on each side were measured as follows.

Sennoside quantitative method: Harvested aerial parts of senna are dried with hot air at 40°C overnight and pulverized with a blender. 100 g of the obtained pulverized material was accurately weighed, 10 ml of 70% methanol was added, the mixture was tightly stoppered and allowed to stand at 40°C overnight, and the resulting filtrate was filtered to determine sennoside A and Quantify B. Note that the conditions for high performance liquid chromatography are as follows.

Column: Lkopxk vskosil 5CB-2
G.

Mobile phase: 2% acetic acid/acetonitrile (14/+6) Column temperature: 40°C Detection: UV275mm Flow rate: 1. Oml/m Example 1 Alexandria senna seedlings (germinated from seeds and grown to a height of 101 cm) - The stem of the plant was coated with urethane 7
Wrap it in an ohm and culture solution (fine powder/-100% of Ibonecus)
It is fixed to the mouth of a wide-mouth bottle (height: 30 cm, opening diameter: 10 cm, capacity: 150011) containing a 0x diluted solution, and ventilation is performed from the bottom of the wide-mouth bottle. Temperature 25℃, illuminance 10゜00
After cultivating for 4 months at 0 lux, the seeds were harvested.

The results were as follows.

Above ground dry weight: 20. Og Sennoside A content: 2. Note: Example 2 Chinneperisenna was cultivated in the same manner as in Example 1, and
Similar measurements were made on the aboveground part. The results were as follows.

Above ground dry weight: 17.8 g Sennoside A content 1 fl. 6
0cm). 10 times a day from the bottom of the aquarium,
Supply the culture medium for 30 minutes at a flow rate of 0.51/min, then stop the pump. When the pump stops, all of the culture solution in the tank is drained out by the siphon, exposing the senna roots to air. 20 tons of balanced culture solution formulated as a culture solution
The lOl is updated once every two weeks using
Cultivate during the power month.

The results of cultivating senna using the above cultivation method were as follows.

Above ground dry weight: 95. Og Sennoside A content: 2.7% by weight Sennoside B content: 3.1% by weight Example 4 When Alexandria senna seedlings and Chinneberry senna seedlings were cultivated in the same manner as in Example 1, they started flowering after about 2 months. After that, it fruited about a month later.

The obtained seeds germinated and grew when sown, so it was confirmed that the hydroponic Senna was fertile.

〔Effect of the invention〕

As described above, according to the present invention, it is possible to always obtain senna and sennosides of stable quality at low cost, and there is no need to rely on natural senna whose quality and supply are unstable.

Agent Patent Attorney Itai - Oboro

Claims (2)

[Claims] (1) A method for cultivating senna characterized by hydroponic senna. (2) A method for producing sennoside, which is characterized by cultivating senna hydrolytically and extracting sennoside from the obtained senna.