JPH01277569A - Apparatus and method for separating blood component - Google Patents

Abstract

PURPOSE:To simplify constitution and to enhance the separation efficiency of blood, by performing the feeding of blood to a blood component separation/ storage container and the return of blood from the storage container to a blood supply source by one pump through the change-over operation of a clamp part. CONSTITUTION:A connection part 30 is connected to a blood supply source and clamp parts 14, 15 are closed while clamp parts 12, 13 are brought to an open state to introduce blood into a blood component separator 4 by a blood feed means 3 through a blood feed tube 2. The blood components flowing out from the outflow ports 23a, 23b of the blood component separator 4 are allowed to flow in a blood component collecting container 10 through a blood feed tube 11 and the blood component flowing out from the outflow port of the separator 4 is allowed to flow in a blood component storage vessel 6 through a blood feed tube 5. After the treatment of blood, the clamp parts 12, 13 are closed and the clamp parts 14, 15 are opened to return the blood component from the separator 4 to the blood supply source.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a blood component separation device and a blood component separation method. In particular, it relates to a single needle blood separation device and separation method.

[Prior Art] Conventionally, a single-needle film-forming type surface liquid separation device and a blood separation method have two or three pumps in the blood circuit: a blood collection pump and a blood auxiliary pump or recirculation pump to adjust the blood circulation speed. Many circuit systems use multiple blood pumps.

As the above type of blood separation device, for example, Japanese Patent Application Laid-open No. 6
There is one shown in Japanese Patent No. 1-109574.

As shown in FIG. 3, this device 100 includes one indwelling needle 102 placed in a blood vessel of a donor (blood donor) D, and a blood pump that pumps blood from this indwelling needle 102 to a deformed plasma separator 103. 110, a return line 105 returns the red blood cell concentrate from which plasma has been separated by the model plasma separation W 103 to the donor D's blood vessel via the indwelling needle 102, and the plasma separator 103. A plasma line 106 transports the flowing plasma to a plasma container 107. Blood supply line 4 has an anticoagulant line 109 that adds anticoagulant from anticoagulant container 108 to blood supply line 4 . The return line 105 includes a variable capacity red blood cell concentrate container 111 inserted into the return line 105 and a return pump 112 disposed downstream of the container 111. The red blood cell concentrate container i 111 is equipped with measuring means 113 for weighing the container 111. This measuring means 113 is connected to a control means 114 which outputs a signal corresponding to the measured value and causes blood to be sent and returned alternately in accordance with this output signal. Further, the return line 105 downstream of the red blood cell concentrate container 111 is branched from the line 105, and the blood supply line 1 downstream of the blood pump 110 is branched from the line 105.
04, and the bypass line 115 is equipped with a return pump 116 that returns the red blood cell concentrate to the blood supply line 104 in conjunction with the return pump 112 by the operation of the control means 114. With this device,
The blood pump 110 is operated until the measured value of the red blood cell concentrate container 111 reaches a specified upper limit, and the return pump 112 and the reverse pump 116 are stopped, and the measured value of the red blood cell concentrated liquid container 111 is determined. When the amount reaches the prescribed upper limit value, the control means 114 stops the blood pump 110 to stop blood feeding according to the upper limit signal from the measuring means 113, and the return pump 1
12 and the reverse pump 116 are operated to perform recirculation and return blood.

On the other hand, as an l-pump type blood separation device using only one blood pump, for example, JP-A-61)-28146
There is one shown in Publication No. 1. As shown in FIG. 4, this device 200 includes an indwelling needle 201 inserted into a blood donor.
, a first blood line 202 that transports venous blood from the indwelling needle 201 to a blood container 209 , and a first blood line 202 that communicates with the first blood line 202 and supplies an anticoagulant from an anticoagulant container 8 to the first blood line 202 . an anticoagulant line 204 that is added by the operation of a coagulant pump 215; a second blood line 2 that communicates with the blood container 209 and transports blood to the membrane-type plasma separator 210;
03, plasma line 205 that introduces plasma flowing out from the deformed plasma separator 210 into the classification container 211, plasma portion 1if
i A red blood cell concentrate line 206 that supplies the red blood cell concentrate from which plasma has been separated from the sac by the device 210 to the red blood cell concentrate container 212; A return line 207 and a first blood line 202 for returning blood to the donor.
The first blood line 202 and the return line 207 are provided with a first clamp means 213 and a second clamp means 214, respectively.

[Problems to be Solved by the Invention] In the device shown in the above-mentioned Japanese Patent Application Laid-Open No. 61-109574, three blood pumps are used, which makes the entire device complicated and large. It is necessary to confirm the switching state of the pump 110 and the pumps 112 and 116, and it is also necessary to confirm the operating state of the pump 112 and the pump 116. This requires a lot of time and effort, and it is difficult to use multiple blood pumps. Therefore, the problem is that the probability of malfunction is high.

Furthermore, in the device shown in the above-mentioned Japanese Patent Application Laid-open No. 60-261461, the device is configured with only one blood pump instead of three pumps as mentioned above. Although there is no problem, with this device, blood collected from a donor passes through the plasma separator 210, and then
All blood is returned directly to the donor. However, the blood that has passed through the plasma separator 210 twice contains a certain amount of plasma components, and is returned to the donor as is.
Blood bone! (Plasma collection) had the problem of poor efficiency.

An object of the present invention is to solve the above-mentioned problems of the prior art, and to be able to perform blood separation, blood feeding to a storage container, and return of blood from the storage container to a blood supply source using a single blood feeding means, and An object of the present invention is to provide a blood component separation device and a blood component separation method that have high separation efficiency.

[Means for Solving the Problems] The present invention provides a connection unit connected to a blood supply source, a blood component separator that separates blood components in blood into a first blood component and a second blood component, a second blood component storage container, a first blood supply tube that communicates with the connecting portion and the blood component separator, and a blood supply means that sends blood to the blood component separator via the first blood supply tube. , a second blood feeding tube that communicates a second blood component outlet provided in the blood component separator with the second blood component storage container, and the blood feeding tube of the second blood component storage container and the first blood feeding tube. a third blood supply tube that communicates with the connection part side from a position where the means is provided; and a third blood supply tube that is located closer to the connection part than the communication part with the third blood supply tube, and the blood component separator. A fourth feeding tube that communicates with the second component outflow port, and a position between a communicating portion between the first feeding tube and the third feeding tube and a communicating portion with the fourth feeding tube. The first to open and close blood vessels
a clamp part, a second clamp part that opens and closes only the second blood vessel, a third clamp part that opens and closes the third blood vessel, a fourth clamp part that opens and closes only the fourth blood vessel, and a first A blood component separation device comprising: a first blood component collection container for storing blood components; and a fifth blood supply tube that communicates a first blood component outlet provided in the blood component separator with the first blood component collection container. It is a device.

Further, what achieves the above object includes the step of connecting the connection part to the blood supply source using the blood component separation device, and closing the third clamp part and the fourth clamp part, and closing the first clamp part and the fourth clamp part. opening the second clamp part, and introducing the blood into the blood component separator by the blood feeding means via the first blood feeding tube, and causing the first blood to flow out from the first blood component outlet of the blood component separator. Flowing the blood component into the first blood component collection container via the fifth blood supply tube,
The second blood component flowing out from the second blood component outlet of the blood component separator
After performing a step of causing the blood component to flow into the second blood component storage container via the second blood supply tube and processing a predetermined amount of blood,
The first clamp part and the second clamp part are in a closed state,
and the step of opening the third clamp part and the fourth clamp part, and the step of re-transferring the blood stored in the second blood component storage container by the blood supply means via the third blood supply tube and the first blood supply tube. Send blood to the component separator and insert the blood component needle ja
causing the first blood component flowing out from the first blood component outlet of the il to flow into the first blood component collection container via the fifth blood supply tube;
The second blood component flowing out from the second blood component outlet of the blood component separator
returning the second blood component to the blood supply source via the fourth blood supply tube, the first blood supply tube and the connecting portion until the blood in the second blood component storage container is almost exhausted; A blood component separation method characterized in that:

Therefore, the blood component separation apparatus of the present invention will be explained using an embodiment shown in FIG.

The blood component separation device 1 of the present invention includes a connection part 30 connected to a blood supply source, a blood component separator 4 that separates blood components in blood into a first blood component and a second blood component, and a second A first blood supply tube 2 that communicates the blood component storage container 6, a connecting portion 30, and the blood component separator 4, and a blood supply means 3 that supplies blood to the blood component separator 4 via the first blood supply tube 2. , a second blood supply tube 5 that communicates the second blood component outlet 22 provided in the blood component separator 4 with the second blood component storage container 6, and the second blood component storage container 6 and the first blood supply tube 2. a third blood supply tube 7 that communicates with the connecting portion 30 side from the position where the blood supply means 3 is provided;
The first blood supply tube 2 and the second component outlet 22 of the blood component separator 6 are located closer to the connection portion 30 than the communication portion 24 with the third blood supply tube 7
The fourth blood supply tube 8 communicates with a first clamp part 12 that opens and closes; a second clamp part 13 that opens and closes only the second blood supply tube 5;
A third clamp part 14 that opens and closes the third blood supply tube 7, a fourth clamp part 15 that opens and closes only the fourth blood supply tube 8, a first blood component collection container lO that stores the first blood component, and a blood component separation First blood component outlet 23a, 2 provided in the vessel 4
3b and the first blood component collection container 10, and a liquid feeding means 9 provided in the fifth blood feeding tube.

The blood component separation device shown in FIG. 1 is applied to a plasma separation device.

The connecting portion 30 is for connecting to an appropriate blood donor means such as a biological vein or a blood bag. As the connection part 30,
If the blood donation means is a biological vein, a blood collection needle is used, and if it is a container containing other blood (for example, a blood bag), a connector connectable to the container is used.

The first feeding tube 2 has a connecting portion 30 attached to its tip, and the other end communicates with the blood component separator 4 . Second blood supply tube 5
The tip communicates with the blood component separator 4, and the other end communicates with the blood inlet of the second blood component storage container 6. The third blood supply tube 7 has a distal end communicating with the blood outflow port of the second blood component storage container 6, and the other end communicating with the first blood supply tube 2 through a communication portion 24. The fourth feeding tube 8 has a distal end connected to the second feeding tube 5 and the communicating portion 2.
6, and as a result, it communicates with the second blood component outlet 22 of the blood component separator 4. Further, the present invention is not limited to this form, and a connector that splits into two may be connected to the second blood component outflow port 22, one of which is connected to the second blood vessel, and the other to the fourth blood vessel. and,
The other end of the fourth blood vessel 8 communicates with the first blood vessel 2 through a communication portion 25 . The fifth feeding tube 11 has its tip connected to the first blood component outflow ports 23a and 23b of the blood component separator 4, and the other end connected to the first blood component storage container 10.
It is preferable that the fifth feeding tube 11 is provided with a liquid feeding means 9 in order to adjust the sampling speed of the first blood component.

The first blood vessel 2 has a communication portion 2 with the third blood vessel 7.
The first clamp portion 12 is provided at a position between the first clamp portion 12 and the communication portion 25 between the first clamp portion 4 and the fourth blood vessel 8 .

Further, the second blood vessel 5 is provided with a second clamp portion 13 that opens and closes only the second blood vessel 5 (without affecting the fourth blood vessel). Further, the third blood vessel 7 is provided with a third clamp portion 14 that opens and closes the third blood vessel. The fourth blood vessel 8 is provided with a fourth clamp portion 15 that opens and closes only the fourth blood vessel 8 (without affecting the second blood vessel).

Furthermore, in the one shown in FIG. 1, a first infusion tube 20 is provided, one end of which communicates with the first blood supply tube 2, and the other end of this first infusion tube 20 is used for brimming and cleaning the inside of the device. A liquid container 19 is provided to store a liquid such as physiological saline for infusion, and the first infusion tube 2o further includes:
A fifth clamp section 21 is provided. Moreover, instead of this clamp part 21, a liquid feeding means may be provided. Further, a second infusion tube 17 is provided, one end of which communicates with the first blood supply tube 2 located on the side of the blood collection means 3o from the communication portion with the first infusion tube 20, and the other end of the second infusion tube 17. The AC
An anticoagulant container 16 containing an anticoagulant such as D solution, CPD solution, and heparin is provided, and the second infusion tube 17 is further provided with a liquid feeding means I8.

The first infusion tube 2, the second infusion tube 5, the third infusion tube 7, the fourth infusion tube 8, the fifth infusion tube 11, the first infusion tube 2o, and the second infusion tube 17 are made of, for example, vinyl chloride resin. A transparent flexible synthetic resin tube can be suitably used.

The first blood feeding tube 2 is provided with a blood feeding means 3. As the blood feeding means 3 and the liquid feeding means 9.18, pumps such as roller pumps and peristaltic pumps are preferably used.

Second blood component storage container 6 and first blood component storage container 1
0 is a soft synthetic resin (e.g. vinyl chloride resin,
formed from ethylene-vinyl acetate resin),
A closed type blood storage means or an open type blood storage means, for example, a hard synthetic resin (e.g., polycarbonate, hard vinyl chloride resin,
Containers made of As resin, ABS resin, etc. can be suitably used.

The blood component separator 4 in the embodiment shown in FIG.
includes a blood inlet, an outlet 22 for a second blood component which is concentrated red blood cells, and a blood separation function that separates the blood into a first blood component (plasma component) and a second blood component (cellular component). , an outlet 23a for the separated first blood component (plasma component)
, 23b is used.

Blood components to be separated are not limited to cellular blood components such as red blood cells, white blood cells, and platelets, and humoral blood components such as plasma, but also large molecular weight proteins such as fibrinogen and IgM in plasma, and small molecular weight proteins such as albumin. Alternatively, there are red blood cells, white blood cells and platelets. Microporous membranes used depending on the blood components to be separated can be used as appropriate. As the structure of the blood component separator, a hollow fiber membrane type, a flat membrane type, etc. are used.

As the first clamp part 12, second clamp part 13, third clamp part 14, fourth clamp part 15, and fifth clamp part 21, forceps or the like may be used, but preferably, forceps or the like may be used. The method is to use a clamp that opens and closes. Furthermore, a switching means 32 is provided for outputting an opening/closing signal to the first clamp section 12, second clamp section 13, third clamp section 14, and fourth clamp section 15, and this switching means 32 is connected to the four clamp sections. are electrically connected, and by selecting the mode (blood collection mode, blood return mode, and furthermore, circulation mode) changeover switch provided on this switching means 32, the four clamp parts can be placed in an open/closed state that matches the desired mode. It may also be possible to switch to

In the blood collection mode, the output signal when the switching means 32 selects the above-mentioned three mode switch (although the circulation mode is not necessarily required) is as follows.
The first clamp part 12 and the second clamp part 13 are in an open state, and the third clamp part 14 and the fourth clamp part 15 are in a closed state.
The clamp part 12 and the second clamp part 13 are closed,
In addition, the third clamp section 14 and the fourth clamp section 15 are opened, and in the circulation mode, the first clamp section 12 and the fourth clamp section 15 are closed, and the second clamp section 13 and the fourth clamp section 15 are closed. 3 to open the clamp portion 14.

Further, in the present invention, as shown in FIG. 1, a blood component separator inlet pressure monitor 26, a blood component separator outlet pressure monitor 27, a 11th liquid acid partial pressure monitor 28, a blood sampling blood pressure monitor 29, etc. are provided. is preferred.

According to the blood component separation device of the present invention, blood separation, blood feeding to the storage container, and blood return from the storage container to the blood supply source can be performed with a single pump by switching the clamp portion described later. , and can perform blood separation with high separation efficiency.

Next, a blood separation method using the blood component separation device of the present invention will be explained using FIG.

The blood separation method of the present invention includes the steps of connecting the connection part 30 to a blood supply source using a blood separation device, closing the third clamp part 14 and the fourth clamp part 15, and closing the first clamp part 12 and the fourth clamp part 15. 2, the step of opening the clamp part 13, and introducing the blood into the blood component separator 4 by the blood feeding means 3 via the first blood feeding tube 2, and the first blood component outflow port 23a of the blood component separator 4. , the first flowing out from 23b
A step of causing the blood component to flow into the first blood component collection container 10 via the fifth blood supply tube 11, and a step of flowing the second blood component flowing out from the second blood component outlet 22 of the blood component separator 4 into the second blood supply tube. 5 into the second blood component storage container 6 and after processing a predetermined amount of blood, the first clamp section 12 and the second clamp section 13 are closed,
and the step of opening the third clamp section 14 and the fourth clamp section 15, and storing the blood components in the second blood component storage container 6 by the blood supply means 3 via the third blood supply tube 7 and the first transfer tube 2. The blood that has been removed is sent to the blood component separator 4 again, and the first blood component outlet 23a of the blood component separator 4,
The first blood component flowing out from 23b is caused to flow into the first blood component collection container IO through the fifth blood supply tube 11, and the second blood component flowing out from the second blood component outlet 22 of the blood component separator 4 is Fourth blood vessel 8, first blood vessel 2, and connection portion 30
The method includes the step of returning blood to the blood supply source via the second blood component storage container until the blood in the second blood component storage container is substantially exhausted.

If the blood supply source is a container containing blood (for example, a blood bag), the step of connecting the connection part to the blood supply source is an operation of communicating the container and the connection part. Specifically, the work involves connecting both connectors and puncturing the connection part to the blood sampling part provided in the container. In addition, when the blood supply source is a living body, puncturing is performed using a connecting portion (puncture needle) to a vein.

connecting the connection 30 to said blood source;
The step of closing the third clamp part 14 and the fourth clamp part 15 and opening the first clamp part 12 and the second clamp part 13 may be performed first.

As shown in FIG. 1, the blood component separation apparatus l includes four clamp parts 12, 13, 14, .
15, when a switch having a switching means 32 that outputs an opening/closing signal is used, the third clamp section 14 and the fourth
The step of closing the clamp part 15 and opening the first clamp part 12 and the second clamp part 13 is an operation of pressing (or switching) the blood collection mode switch in the switching means 32. Similarly, the step of closing the first clamp section 12 and the second clamp section 13 and opening the third clamp section 14 and the fourth clamp section 15 involves pressing the blood return mode switch in the switching means 32. (or switching).

In addition, the first blood component flowing out from the blood component separator 4 is
It is preferable to use the liquid feeding means 9 to flow into the first blood component storage container.

Therefore, in the blood component separation method of the present invention, when blood is collected, blood supply source→connection part 30→first blood supply tube 2→
A circuit including blood feeding means 3 → blood component separator 4 → second blood feeding tube 5 → second blood component storage container 6 is configured, and the clamp 12
．． 13, 14, and 15, when blood is returned, the second blood component storage container 6 → the third blood supply tube 7 → the first blood supply tube 2 → the blood supply means 3 → the blood component separator 4 → the fourth blood supply A circuit consisting of blood vessel 8 → first blood supply tube 2 → connection part 30 → blood supply source is configured, and one blood supply means can perform blood collection and blood return, making it easy to control the blood supply means.

Furthermore, when blood is returned, the returned blood cell components pass through the blood separator again, resulting in high plasma collection efficiency.

Then, the above-mentioned blood collection and blood return are repeated one or more times to collect a required amount of the first blood component.

Therefore, according to the blood component separation method of the present invention, blood component separation, blood feeding to the storage container, and blood return from the storage container to the blood supply source can be performed with one blood feeding means, and separation efficiency can be improved. can perform high blood separation.

Furthermore, if necessary, inside the second blood component storage container 6,
After storing the required amount of the second blood component and before performing the blood return operation, a step of circulating the second blood component stored inside the storage container 6 to the blood component separator 4 may be performed. In this case, the clamp parts are operated by closing the first clamp part 12 and the fourth clamp part 15, opening the second clamp part 13 and the third clamp part 14, and closing the first clamp part 12 and the fourth clamp part 15, and opening the third clamp part 13 and the third clamp part 14. The blood stored in the second blood component storage container 6 is sent again to the blood component separator 4 by the blood feeding means 3 via the blood feeding tube 2, and the first blood component outflow port 22a of the blood component separator 4 is , the first flowing out from 22b
The blood component flows into the first blood component collection container 1° via the fifth blood supply tube 11, and the second blood component flowing out from the second blood component outlet 22 of the blood component separator 4 is passed through the second blood supply tube 5. The blood components may be circulated into the second blood component storage container 6 through the blood component storage container 6. By performing this circulation, blood components can be separated more reliably.

During this circulation, the second blood component storage container 6-third
A circuit consisting of the blood feeding tube 7 → first blood feeding tube 2 → blood feeding means 3 - blood component separator 4 → second blood feeding tube 5 → second blood component storage container 6 is configured, and one blood feeding means collects and returns blood. Blood and circulation can be carried out.

Further, as described above, when the switching means 32 (having a circulation mode switch) is used, the clamp switching operation is an operation of pressing or switching the circulation mode switch in the switching means 32.

[Example] A blood component separator having the configuration shown in Fig. 1 was used, and the blood component separator was a plasma separator for separating plasma proteins. Fresh bovine blood 412 (Hct 40%) was used.

The blood sampling speed is 50x (1/min), and one blood sampling is 120oz.
(7, the blood return rate is 15 x Q/min, and the liquid feeding rate of the liquid feeding means for collecting plasma is 13 x (1/min,
The blood return time was set to 20xQ1 min. and an inlet pressure P1 measured by a blood component separator inlet pressure monitor;
Outlet pressure Po1 measured by the blood component separator outlet pressure monitor; Filtration pressure P measured by the plasma component pressure monitor.
While monitoring F, the filtration differential pressure PF-PO is -30x
When zH9 is reached, the speed of the liquid feeding means is decreased by 2xU, and if PF-P O≦-30m19 after 5 seconds, the speed is further decreased by 2 passes/min. 5 seconds after decreasing the speed of PF-P
O2-10xil (in the case of IF, 1 ff12/min at the time of blood collection and 20zQ1 min at the time of blood return)
Blood separation was performed while controlling the temperature to increase by minutes. As a result, the time it took for 400z(l) of plasma to be collected was 28 minutes and 07 seconds.

As the plasma separator, a donut-shaped flat membrane stacked plasma separator (membrane area: 0.2a+'') was used.

Soft vinyl chloride resin bags were used as the plasma component storage container and the blood component storage container.

A roller pump was used as a blood feeding means.

(Comparative Example) FIG. 2 shows a configuration diagram of a blood component separation device 5o of a comparative example.

This blood component separation device 5o uses only one blood feeding means, passes through the blood component separator 4 only when blood is collected, and bypasses the blood component separator 4 when returning blood. From the branch point 33 between 4 and 4, through the feeding tube 8,
5 into the first blood supply tube 2, and the blood is returned to the blood supply source through the connecting portion 3o without undergoing any separation operation.

The blood component separation device of this comparative example is configured so that a blood separation operation cannot be performed when blood is returned.

Using this comparative example, plasma separation was performed under the same conditions and using the same blood component separator as in the example, using ACD1 heparinized fresh cow 111114f2 (Hat 40%) as a blood source.

The blood collection speed is 5017 minutes, and one blood collection i! 200x(!
The blood return rate was set to 15x (1/min), the speed of the liquid feeding means was set to 2 oz (17 minutes) at the time of blood collection, and the speed was controlled in the same manner as in the example.

As a result, it took 33 minutes to collect 400,112 plasma samples.
It took 0 seconds.

[Effects of the Invention] The blood component separation device of the present invention includes: a connection portion connected to a blood supply source; a blood component separator that separates blood components in the blood into a first blood component and a second blood component; a second blood component storage container, a first blood supply tube that communicates with the connecting portion and the blood component separator, and a blood supply means that sends blood to the blood component separator via the first blood supply tube. , a second blood feeding tube that communicates a second blood component outlet provided in the blood component separator with the second blood component storage container, and the blood feeding tube of the second blood component storage container and the first blood feeding tube. a third blood supply tube that communicates with the connection part side from a position where the means is provided; and a third blood supply tube that is located closer to the connection part than the communication part with the third blood supply tube, and the blood component separator. A fourth feeding tube that communicates with the second component outflow port, and a position between a communicating portion between the first feeding tube and the third feeding tube and a communicating portion with the fourth feeding tube. a first clamp part that opens and closes the blood vessel; a second clamp part that opens and closes the second blood vessel without affecting the fourth blood vessel; a third clamp part that opens and closes the third blood vessel; without affecting the second blood supply tube (a fourth clamp part that opens and closes the fourth blood supply tube; a first blood component collection container that stores the first blood component; and a second blood supply tube provided in the blood component separator; Since the device has a fifth blood feeding tube that communicates the first blood component outlet and the first blood component collection container, the device is provided with only one blood feeding means, so the entire device is There is no need to increase the size of the device, the circuit configuration of the device can be made simple, and the blood feeding means can be easily managed.Furthermore, blood components can be separated and transferred to the storage container by switching the clamp part. Blood can be returned from the blood and storage container to the blood supply source using one pump, and blood can be separated with high separation efficiency.

Further, the blood separation method of the present invention includes the step of connecting a connection part to a blood supply source using the blood component separation device;
The third clamp part and the fourth clamp part are in a closed state,
opening the first clamp section and the second clamp section; introducing blood into the blood component separator by the blood feeding means via the first blood feeding tube; and introducing the blood into the blood component separator through the first blood component outlet of the blood component separator. The first blood component flowing out through the fifth blood supply tube is caused to flow into the first blood component collection container, and the second blood component flowing out from the second blood component outlet of the blood component separator is passed through the second blood supply tube. After performing the step of causing blood to flow into the second blood component storage container through the blood component storage container and processing a predetermined amount of blood, the first clamp part and the second clamp part are closed, and the third clamp part and the fourth clamp part are closed. through the step of opening the third blood vessel and the first blood vessel;
The blood stored in the second blood component storage container is sent to the blood component separator again by the blood feeding means, and the first blood component flowing out from the first blood component outlet of the blood component separator is transferred to the fifth blood feeding tube. The second blood component flows into the first blood component collection container through the second blood component collection container, and the second blood component flows out from the second blood component outlet of the blood component separator. This method is characterized by the step of returning blood to the blood supply source via the second blood component storage container until the blood in the second blood component storage container is almost exhausted. Blood can be sent to the storage container and returned from the storage container to the blood supply source using - blood feeding means, and the blood feeding means can be easily managed. Since the second blood component passes through the blood component separator again, it is possible to perform blood separation with high separation efficiency.

[Brief explanation of the drawing]

Fig. 1 is a block diagram showing an embodiment of the blood component separation device of the present invention, Fig. 2 is a block diagram showing a blood adult bone separation device of a comparative example, and Fig. 3 is a conventional blood separation device. FIG. 4 is a block diagram showing a conventional blood separation device. 1...Blood component separator, 30...Connection part, 2.
...First blood supply tube, 3.Blood feeding means, 4.Blood component separator, 5.Second blood supply tube, 6.Second blood component storage container, 7.Third. Blood vessel, 8... Fourth blood vessel, 9...
...Liquid feeding means, 10...First blood component collection container, II
...Fifth blood supply tube, 12...First clamp part, 1
3... Second clamp part, 14... Third clamp part,
15... Fourth clamp part, 16... Anticoagulant container,
17... Second infusion tube, 18... Liquid feeding means, 19
...Liquid container, 20...First infusion tube, 21.
...Fifth clamp part, 22...Second blood component outflow ports 23a, 23b...First blood component outflow ports 24, 25
．． 26... Communication portion 26... Blood component separator inlet pressure monitor 27... Surface liquid acid separator 14 unit outlet pressure monitor 28... First blood component pressure monitor

Claims (2)

[Claims] (1) A connecting portion connected to a blood supply source, a blood component separator that separates blood components in blood into a first blood component and a second blood component, a second blood component storage container, and the connecting portion a first blood supply tube that communicates with the blood component separator, a blood supply means for sending blood to the blood component separator via the first blood supply tube, and a first blood supply tube provided in the blood component separator. 2. A second blood supply tube that communicates between the second blood component outlet and the second blood component storage container, and a second blood component storage container and the first blood supply tube closer to the connection portion than the position where the blood feeding means is provided. a fourth blood supply tube that communicates with a second component outlet of the blood component separator and the first blood supply tube at a position closer to the connection part than the communication part with the third blood supply tube; a first clamp portion that opens and closes the first blood vessel at a position between the first blood vessel and the third blood vessel and a communication portion between the fourth blood vessel; a second clamp part that opens and closes only the second blood supply tube, a third clamp part that opens and closes the third blood supply tube, a fourth clamp part that opens and closes only the fourth blood supply tube, and stores the first blood component. Blood component separation characterized by having a first blood component collection container, and a fifth blood supply tube that communicates a first blood component outlet provided in the blood component separator with the first blood component collection container. Device. (2) Using the blood component separation device of claim 1, connecting the connection part to the blood supply source, and closing the third clamp part and the fourth clamp part, and closing the first clamp part and the second clamp part. a step of opening the first part;
Blood is introduced into the blood component separator by the blood feeding means via the blood feeding tube, and the first blood component flowing out from the first blood component outlet of the blood component separator is transferred to the first blood via the fifth blood feeding tube. a step of causing the second blood component flowing into the component collection container and flowing out from the second blood component outlet of the blood component separator into the second blood component storage container via the second blood supply tube; After performing the treatment, the first clamp part and the second clamp part are closed, and the third clamp part and the fourth clamp part are opened, and the third clamp part and the first clamp part are opened. , the blood stored in the second blood component storage container is fed again to the blood component separator by the blood feeding means, and the first blood component flowing out from the first blood component outlet of the blood component separator is sent to the fifth blood component. 1st via blood vessels
The second blood component flows into the blood component collection container and flows out from the second blood component outlet of the blood component separator. 2. Returning blood to the blood supply source until the blood in the blood component storage container is almost exhausted.