JPH01165933A - Quartz vibrator for chemical measurement - Google Patents

Abstract

PURPOSE:To analyze a very small amount of a specimen, by integrating a specimen cell with a quartz vibrator and allowing the bonded part of both of them to penetrate in the inner wall of the specimen cell. CONSTITUTION:A specimen cell 3 and a quartz vibrator 1 are bonded by an adhesive and the bonded part is partially penetrated in the inner wall of the specimen cell 3. By this method, the leakage of a specimen 4 is held in the specimen cell 3 without being leaked. As a result, a very small amount of the specimen of 0.05ml or less can be analyzed.

Description

[Detailed Description of the Invention] [Field of Industrial Application] The present invention is applicable to chemical reaction tracking and state analysis in the fields of chemistry, physical chemistry, biochemistry, medicine, food, and chemical industry, and chemical measurement using the same. It is related to the device that performs this.

[Summary of the invention]

The present invention, in a crystal resonator for chemical measurements, integrates a sample cell and a crystal resonator, and also allows the joints of these to penetrate inside the inner wall of the sample cell to prevent sample leakage and prevent the crystal from leaking. This reduces the influence of surrounding parts on the vibration of the vibrator, simplifies the operation during measurement, and allows for inexpensive manufacturing.

[Conventional technology]

Conventionally, in chemical measurement crystal resonators, chemical measurements have been performed by sandwiching the crystal resonator between O-rings or the like, injecting a sample into the inside of the O-ring, and measuring the change in frequency of the crystal resonator.

[Problem that the invention seeks to solve]

However, in the conventional technology, since the O-ring is pressed against the vibration surface of the crystal resonator, the vibration of the crystal resonator is affected thereby. Additionally, there is a gap between the O-ring and the crystal oscillator, and the sample leaks through the gap, which limits the ability to measure small amounts of samples.

Furthermore, as a pre-measurement operation, it is necessary to press O-rings against both sides of the crystal resonator each time, and the manufacturing cost is also high due to the large number of components of the device.

[Failure to solve the problem]

Therefore, in the present invention, the sample cell and the crystal resonator are integrated, and the joint part of the sample cell and the crystal resonator is inserted into the inner wall of the cell, so that the crystal resonator is influenced by the surrounding parts. It has been made possible to reduce the amount of water, manufacture it at low cost with simple operations, and measure minute amounts of samples.

[Effect]

By using a crystal resonator for chemical measurements configured as described above, the frequency of the crystal resonator changes depending only on the sample, and the sample does not leak from the cell, making it possible to measure small amounts of samples. Furthermore, the measuring device can be manufactured easily and at low cost.

〔Example〕

Embodiments of the present invention will be described below based on the drawings.

FIG. 1 shows a chemical measurement crystal resonator according to the present invention. A sample (glycerin aqueous solution) cell 3 is bonded to the outer edge of a crystal resonator 1 with electrodes 2 deposited on both sides by adhesive. Part of the joint part is sample cell 3.
It has penetrated into the inner wall of. The crystal resonator 1 is a device that utilizes a piezoelectric effect, and causes mechanical vibration by applying a voltage with a frequency near the resonance frequency.

Although this vibration is extremely small, it is resisted by interaction with the aqueous glycerin solution in contact with it.

The resistance coefficient of this mechanical resistance can be considered to correspond to the electrical resistance if the mechanical vibration and electrical vibration of the crystal resonator are considered in correspondence. Therefore, the loss resistance at the resonance frequency is considered to be a value reflecting the friction coefficient of the vibrator surface, and by measuring this loss resistance, it was possible to measure the viscoelasticity of the glycerin aqueous solution.

(Example 1) In this example, a case will be described in which a sample is held in a part where a sample cell and a crystal resonator are integrated. FIG. 2 shows a crystal resonator for chemical measurements according to this embodiment. The sample cell 3 and the crystal resonator 1 are bonded together with an adhesive, and the bonded portion partially enters the inner wall of the sample cell 3. Thereby, the sample 4 is held within the sample cell 3 without leaking. By doing so, it was possible to analyze a trace sample of 0.05 m 1 or less.

(Example 2) In this example, a case will be described in which the area and position of the joint between the sample cell and the crystal resonator do not affect the frequency of the crystal resonator. FIG. 3 is an enlarged view of the part where the sample cell is connected to the crystal resonator. The sample cell 3 is joined to the crystal resonator 1 at the outer edge 5 of the crystal resonator 1 .

The negative portion of the outer edge portion 5 enters the inner wall of the sample cell 3. The crystal resonator 1 can vibrate freely except for the outer edge 5, and the influence of the surroundings is suppressed to a low level. This made it possible to measure with high precision over a wide viscosity range.

(Example 3) In this example, a case will be described in which the chemical measurement to be performed is a viscoelasticity measurement. FIG. 4 shows a crystal resonator for chemical measurement according to this embodiment.

For example, a crystal resonator causes mechanical vibrations each time a voltage with a frequency near the resonance frequency is applied. This vibration occurs when the sample 4 and the crystal oscillator 1 are in contact with each other.
It is resisted by the shear center force between it and the surface. Since this mechanical resistance can be considered in correspondence to electrical resistance, the viscoelasticity of Sample 4 could be measured by measuring the loss resistance at the resonance frequency.

(Example 4) In this example, a case where the chemical measurement to be performed is an immunoassay will be described. The crystal oscillator 1 has its resonant frequency 1
The resonant frequency of mechanical vibration caused by applying a voltage of a nearby frequency is affected by the surface weight of the vibrator. Changes can be measured. FIG. 5 shows a chemical measurement crystal resonator according to this embodiment. An anti-human immunoglobulin G antibody is immobilized on the surface of the crystal resonator 1. When human immunoglobulin G is injected as sample 4, human immunoglobulin G is selectively adsorbed to the surface of crystal resonator 1 due to antigen-antibody reaction. Since the weight change caused by this was reflected in the vibration frequency, the concentration of human immunoglobulin G could be measured by measuring the change in the vibration frequency of the crystal resonator 1.

(Example 5) In this example, a case will be described in which the chemical measurement to be performed is automatic and continuous. FIG. 6 shows a chemical measurement crystal resonator according to this embodiment. The sample cell 3 has two flow tubes 6 (t+3), one of which is used as a sample inlet and the other (7-) as a sample outlet.
flowing continuously through the The crystal resonator 1 causes mechanical vibration by being applied with a voltage having a frequency near the resonance frequency. This vibration is resisted by the interaction between the crystal resonator 1 and the sample 4. Since this mechanical resistance can be considered in correspondence with electrical resistance, the viscoelasticity or antigen concentration of the sample 4 can be measured by measuring the loss resistance at the resonance frequency. Sample 4
passed through the flow tube 6 and interacted continuously with the crystal oscillator 1, making it possible to measure continuous changes in its viscoelasticity or antigen concentration.

(Example 6) In this example, the object of the chemical measurement to be performed is a chemical reaction. FIG. 7 shows a crystal resonator for chemical measurement according to this embodiment. , a flow tube 6 is attached to the sample cell 3 at two locations, and one of the flow tubes is used as a sample inlet and the other as a sample outlet.
By measuring the loss resistance or frequency change at the resonance frequency, the viscoelasticity or antigen concentration of the sample 4 can be continuously measured. When the state of sample 4 changes over time due to a chemical reaction, the chemical reaction could be traced.

〔Effect of the invention〕

As explained above, the present invention uses a crystal oscillator for chemical measurements in which a sample cell and a quartz crystal oscillator are integrated, thereby making it possible to perform highly accurate measurements of quantitative samples, and the measuring instrument itself is easy to operate and inexpensive. It is designed so that it can be manufactured in

[Brief explanation of the drawing]

FIG. 1 is an explanatory diagram of a chemical measurement crystal resonator according to the present invention, and FIGS. 2 to 7 are explanatory diagrams of chemical measurement crystal resonators according to Examples 1 to 6, respectively. ■...Crystal oscillator 3...Sample cell 4...Sample 5...Crystal oscillator outer edge

Claims (8)

[Claims] (1) In a chemical measuring device that uses a crystal resonator as a detector, the sample cell and the crystal resonator are integrated, and the joint portion of the sample cell and the crystal resonator is connected to the inner wall of the sample cell. A crystal oscillator for chemical measurement, characterized in that it spans the inside of the oscillator. (2) The crystal resonator for chemical measurement according to claim 1, characterized in that the sample cell and the crystal resonator are integrated into a structure that holds a sample. (3) A joint portion between the sample cell and the crystal resonator,
The crystal resonator for chemical measurement according to claim 1 or 2, characterized in that the area and position are such that it does not affect the vibration of the crystal resonator. (4) A joint portion between the sample cell and the crystal resonator,
A crystal resonator for chemical measurement according to any one of claims 1 to 3, characterized in that the outer edge portion of the crystal resonator is an outer edge portion of the crystal resonator. (5) The crystal resonator for chemical measurement according to any one of claims 1 to 4, wherein the crystal resonator is an AT-cut crystal resonator. (6) Claims 1 to 5, characterized in that the chemical measurement performed by the chemical measuring device is viscoelasticity measurement.
A crystal resonator for chemical measurements as described in any of the preceding paragraphs. (7) The chemical measurement crystal resonator according to any one of claims 1 to 6, wherein the chemical measurement performed by the chemical measurement device is an immunoassay. (8) Claim 1, characterized in that the chemical measurement performed by the chemical measuring device is automatic and continuous.
A crystal resonator for chemical measurement according to any one of paragraphs 7 to 7.