JP7561478B2 - Oral cavity composition for inhibiting stain adhesion - Google Patents

Description

The present invention relates to an oral composition for inhibiting stain adhesion.

Stains, which are discolored stains on teeth, are formed when coloring substances such as curcumin and aminocarbonyl reactants contained in food, or coloring substances such as tar from cigarettes, combine with the pellicle, a salivary component that covers the surface of tooth enamel, and adhere to the teeth.

With the growing awareness of oral aesthetics in recent years, stains have become a major aesthetic issue. For this reason, various methods aimed at removing stains from teeth have been developed and investigated. For example, Patent Document 1 discloses a dentifrice composition that contains four components, including polyphosphate, and has a high stain-removing effect. Patent Document 2 discloses an oral composition for removing stains that contains sodium metaphosphate. Thus, although technologies aimed at removing stains from teeth are known, no oral composition aimed at inhibiting the adhesion of stains to teeth has been known.

JP 2013-112654 A JP 2015-137274 A

The present invention aims to provide an oral composition that can suppress staining of teeth caused by smoking tobacco or ingesting stain-causing foods.

The inventors have now discovered that the protein components of collagen, casein and whey effectively inhibit staining caused by tobacco and stain-causing foods. The present invention is based on this finding.

According to the present invention, the following inventions are provided.
[1] An oral composition and an agent for inhibiting stain adhesion, which contain one or more components selected from the group consisting of collagen, casein and whey (hereinafter, sometimes referred to as the "oral composition and agent of the present invention").
[2] The composition and agent described in [1] above, wherein the staining is caused by smoking tobacco or eating stain-causing foods.
[3] The composition and preparation described in [1] or [2] above, which is a beverage.
[4] The composition and agent described in [3] above, wherein the beverage is a tea beverage or a coffee beverage.
[5] The composition and preparation described in [4] above, wherein the tea beverage is a beverage selected from the group consisting of black tea beverages, oolong tea beverages, green tea beverages, grain tea beverages, rooibos tea beverages, and blended tea beverages thereof.
[6] A method for producing a beverage for inhibiting stain adhesion, comprising blending one or more components selected from the group consisting of collagen, casein and whey.

The oral composition and preparation of the present invention can be taken together with smoking tobacco or eating stain-causing foods to suppress staining caused by tobacco or said foods. The oral composition and preparation of the present invention can also be provided in the form of a beverage, which is advantageous in that it allows for easy prevention of staining caused by tobacco or said foods in daily life.

Description of the Invention

The oral composition and preparation of the present invention contain one or more protein components selected from the group consisting of collagen, casein, and whey as an active ingredient.

The collagen that is the active ingredient of the present invention may be collagen extracted from collagen tissue (e.g., skin) of livestock (e.g., cows, pigs) or poultry (e.g., chickens), or collagen extracted from collagen tissue of fish, and is not particularly limited. Commercially available collagen can also be used.

The collagen, which is the active ingredient of the present invention, can be dissolved in the oral composition before use, so water-soluble collagen can be used. Here, water-soluble collagen means insoluble natural collagen that has been treated to become water-soluble. Examples of water-soluble collagen include gelatin obtained by thermally denaturing natural collagen, and collagen peptides obtained by hydrolyzing natural collagen or gelatin, and these may also be further processed (for example, enzyme-modified or chemically modified).

The molecular weight of the collagen is not particularly limited, but considering that it will be dissolved in the oral composition for use, for example, collagen with a weight average molecular weight of 500 to 300,000 (preferably 1,000 to 200,000, more preferably 3,000 to 20,000) can be used.

In the present invention, collagen peptides, which are hydrolysates of collagen or gelatin, can be used as active ingredients. That is, in the present invention, "collagen" is used to mean collagen peptides. Collagen peptides are peptides obtained by hydrolyzing collagen or gelatin with an enzyme or acid, and commercially available products can also be used.

The molecular weight of the collagen peptide is not particularly limited, but considering that it will be dissolved in the oral composition for use, it is possible to use one with a weight average molecular weight of, for example, 500 to 300,000 (preferably 1,000 to 200,000, more preferably 3,000 to 20,000). Here, the weight average molecular weight of the collagen and collagen peptide in the present invention refers to the value determined by gel permeation chromatography (GPC) analysis using pullulan for molecular weight measurement as the standard, and can be measured, for example, by the method described in the 10th edition of the Photographic Gelatin Test Method (PAGI method).

Casein, the active ingredient of the present invention, is the main protein component contained in milk and is known to account for approximately 80% of cow's milk protein. In the present invention, in addition to casein isolates (e.g., natural cheese), casein concentrates, dried products, frozen products and hydrolysates thereof can be used. Examples of casein proteins include α-casein, β-casein and κ-casein. Commercially available casein products can be used in the present invention.

Whey, which is an active ingredient of the present invention, is an aqueous solution obtained after removing the curds that are produced by adding rennet or acid to milk such as cow's milk, and is also called whey. In the present invention, in addition to the original whey liquid, its concentrate, dried product (e.g., whey powder), frozen product, and hydrolysate can be used, and further, whey protein contained in whey can be used. Examples of whey protein include whey protein concentrate (WPC), whey protein purified product (WPI), α-lactalbumin (α-La), β-lactoglobulin (β-Lg), immunoglobulin, and lactoferrin. In the present invention, commercially available whey can be used.

The composition and agent of the present invention can be provided as one or more active ingredients selected from the group consisting of collagen, casein, and whey, or as a mixture of the active ingredients and other ingredients (e.g., food additives, formulation additives). In the present invention, the agent of the present invention can be made of one or more active ingredients selected from the group consisting of collagen, casein, and whey, and the composition of the present invention can be made of one or more active ingredients selected from the group consisting of collagen, casein, and whey, and other ingredients.

The collagen concentration (solid content equivalent) in the oral composition and preparation of the present invention is not particularly limited as long as the stain adhesion inhibitory effect is exhibited, but for example, the lower limit of the collagen concentration can be 0.02 mass% (preferably 0.05 mass%) and the upper limit of the concentration can be 2.00 mass% (preferably 1.00 mass%). The collagen concentration in the oral composition and preparation of the present invention can be 0.02 to 2.00 mass%.

The casein concentration (solid content equivalent) in the oral composition and preparation of the present invention is not particularly limited as long as the stain adhesion inhibitory effect is exhibited, but for example, the lower limit of the casein concentration can be 0.02% by mass (preferably 0.05% by mass) and the upper limit of the concentration can be 2.00% by mass (preferably 1.00% by mass). The casein concentration in the oral composition and preparation of the present invention can be 0.02 to 2.00% by mass.

The whey concentration (solid content equivalent) in the oral composition and preparation of the present invention is not particularly limited as long as the stain adhesion inhibitory effect is exhibited, but for example, the lower limit of the whey concentration can be 0.02 mass% (preferably 0.05 mass%) and the upper limit of the concentration can be 2.00 mass% (preferably 1.00 mass%). The whey concentration in the oral composition and preparation of the present invention can be 0.02 to 2.00 mass%.

When the oral compositions and preparations of the present invention are provided in the form of a liquid, semi-liquid, gel, or the like, the pH can be adjusted to a range of 4.0 to 8.0. The pH of the oral compositions and preparations of the present invention may be adjusted by the same method for adjusting the pH of food, for example, by using sodium bicarbonate. The pH of beverages can be measured using a commercially available pH meter.

The oral composition and preparation of the present invention are used to inhibit stain adhesion. In the present invention, "stain adhesion" refers to stain adhesion to the teeth of mammals, including humans (particularly human teeth). In the present invention, "stain adhesion" refers to a discoloration phenomenon caused by inhalation or ingestion through the oral cavity. Causes of stain adhesion include smoking tobacco and ingestion of stain-adhering foods, and causative substances include tar, aminocarbonyl reactants, curcumin, anthocyanin, lycopene, and carotenoids. Foods containing aminocarbonyl reactants include sauces and syrups, and foods containing curcumin include curry.

In the present invention, "inhibition of stain adhesion" refers to the inhibition of adhesion to teeth of stain-causing substances (e.g., tar, aminocarbonyl reaction products, curcumin, anthocyanin, lycopene, carotenoids) contained in tobacco or foods (e.g., sauces, curries, fruits, green and yellow vegetables).

The oral compositions and preparations of the present invention can be applied to or ingested in the oral cavity before, during, and/or after smoking a cigarette, to better suppress stain deposition caused by smoking a cigarette, and are preferably applied to or ingested in the oral cavity just before, during, and/or just after smoking a cigarette. The oral compositions and preparations of the present invention can also be applied to or ingested in the oral cavity before, during, or after ingestion of a food containing a stain-causing substance, to better suppress stain deposition caused by ingestion of the food, and are preferably applied to or ingested in the oral cavity just before, during, and/or just after ingestion of the food.

In the present invention, stain adhesion can be determined by measuring the color difference between the tooth surface before immersion in the test composition and the tooth surface after immersion in the test composition in the presence of a staining substance using the L*a*b* color system (JIS Z8722:2009) (see Example 1 (3) in the Examples below). When the color difference between the tooth surface before immersion in the test composition and the tooth surface after immersion in the test composition in the presence of a staining substance is reduced compared to when the tooth surface is immersed in the control composition, it can be determined that the test composition inhibits stain adhesion. The color difference of the tooth surface can be determined, for example, by a spectrophotometer. For example, hydroxyapatite pellets (manufactured by Pentax Corporation) can be used as the tooth used for measurement.

In the present invention, it can be determined that stain adhesion has been inhibited when the color difference Δb between the tooth surface before immersion in the test composition and the tooth surface after immersion in the test composition in the presence of a staining substance is smaller than the color difference Δb between the tooth surface after immersion in the control composition.

The oral composition and preparation of the present invention are not particularly limited in their form or shape, and may be in the form of a liquid, semi-liquid, gel, or the like, so long as they have the effect of inhibiting stain adhesion. The oral composition and preparation of the present invention may be provided in the form of a food composition, such as a beverage or jelly.

When the oral composition and preparation of the present invention are provided in the form of a beverage, the beverage may be any beverage that can contain protein components such as collagen, and examples of such beverages include tea beverages, coffee beverages, and soft drinks. Examples of tea beverages that can be used in the present invention include fermented tea (e.g., black tea) beverages, non-fermented tea (e.g., green tea, rooibos tea) beverages, semi-fermented tea (e.g., oolong tea) beverages, post-fermented tea (e.g., pu-erh tea) beverages, and grain tea (e.g., barley tea) beverages, as well as blends of these tea beverages.

The beverage of the present invention may contain beverage additives that are typically used in the manufacture of beverages, such as sweeteners, acidulants, flavorings, colorings, fruit juice, and food additives (e.g., foaming and foam retention enhancers, bittering agents, preservatives, antioxidants, thickening stabilizers, emulsifiers, dietary fiber, and pH adjusters).

The pH of the beverage of the present invention can be adjusted by the same techniques used to adjust the pH of ordinary beverages, for example, by using sodium bicarbonate.

The beverage of the present invention can be provided in the form of a packaged beverage. The container for the packaged beverage means a sealed container that can prevent the contents from contacting the outside air, and examples of such containers include metal cans, barrel containers, plastic bottles (e.g., PET bottles, cups), paper containers, bottles, and pouch containers.

The beverage of the present invention can be produced according to a conventional method used in producing beverages, except that one or more protein components selected from the group consisting of collagen, casein, and whey are added to the raw materials. For example, when the beverage of the present invention is a tea beverage, a tea beverage for inhibiting stain adhesion can be obtained by preparing a tea extract and mixing the active ingredient. In producing the beverage of the present invention, beverage additives used in the formulation of conventional beverages may be added, and there is no particular restriction on the timing of adding these additives.

The beverage of the present invention can be produced using production techniques known in the art, for example, "Revised New Edition Soft Drinks" (Korin Co., Ltd.) can be used as a reference.

In the manufacturing process for the beverage of the present invention, the timing of addition of the protein component selected from the group consisting of collagen, casein, and whey is not particularly limited, and it can be added, for example, during the raw material blending process. The step of adding the protein component and the step of adding other blended components such as beverage additives may be performed simultaneously or separately, and if performed separately, either may be performed first.

The beverage provided by the present invention can be provided as a bottled beverage through processes such as an extraction process, a blending process, a filling process, and a sterilization process. For example, the beverage obtained in the blending process can be sterilized in a conventional manner and filled into a container. Sterilization may be performed before or after filling into the container.

When sterilization is performed in the production of the beverage of the present invention, various sterilization methods commonly used in the food industry can be used, and typically heat sterilization can be used. Examples of sterilization methods that can be used include retort sterilization, UHT sterilization, low-temperature sterilization, and HTST sterilization. These heat sterilization methods can be carried out under conditions that are commonly used in the production of beverages.

According to another aspect of the present invention, there is provided a method for producing a beverage for inhibiting stain adhesion, comprising blending one or more protein components selected from the group consisting of collagen, casein, and whey. The production method of the present invention can be carried out according to the description of the oral composition and beverage of the present invention and the production thereof.

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples.

Example 1: Stain adhesion suppression effect of protein (1)
(1) Preparation of tobacco extract 50 g of milliQ water was placed in a suction filter bottle, and a rubber stopper with a glass tube was attached. A cigarette (Marlboro gold 100's (registered trademark), manufactured by Philip Morris Co.) was inserted into the tip of the glass tube, ignited, and immediately sucked with a pump. The cigarette was burned until it was 5 cm long from the tip, and sucking was stopped. The same operation was repeated for a total of 10 cigarettes. The liquid was collected from the suction filter bottle and used as a tobacco extract.

(2) Preparation of protein solutions Three types of commercially available collagen (Iquos HDL-50DR (Nitta Gelatin Co., Ltd., weight average molecular weight: 5000), Super Collagen Peptide SCP-2000 (Nitta Gelatin Co., Ltd., weight average molecular weight: 1100), Water-soluble Collagen Peptide SS (Kyowa Hakko Bio Co., Ltd., weight average molecular weight: 6600)) and three types of milk proteins (high whey protein WPI895 (Fonterra Co., Ltd.), casein Na (Fonterra Co., Ltd.), lysozyme (Wako Pure Chemical Industries, Ltd.)) were dissolved in water to a concentration of 0.2% by mass (solid content equivalent) to prepare each protein solution. Each protein solution was filled into a can and retorted at 121 ° C. for 7 minutes. The pH of each protein solution after sterilization was measured using a pH meter (Toa DKK Co., Ltd.).

(3) Coloring test Each test solution was prepared by mixing the tobacco extract obtained in (1) above with each protein solution obtained in (2) above at a ratio of 1:1 (by mass). A control solution was prepared by mixing the tobacco extract obtained in (1) above with water at a ratio of 1:1 (by mass). The stain adhesion caused by each test solution was evaluated using hydroxyapatite pellets (APP-100, PENTAX, hereinafter referred to as "HAP"). After measuring the color of the HAP surface using a spectrophotometer (SE7700, Nippon Denshoku Industries Co., Ltd.), the HAP was immersed in artificial saliva (50 mM potassium chloride, 1 mM calcium chloride, 0.1 mM magnesium chloride, 1 mM potassium dihydrogen phosphate, 1% albumin, pH 7.0) at 37°C for 7 hours. After the immersion, the HAP was washed with total ion-exchanged water, and then immersed in each test solution at 37°C for 24 hours. The surface of the HAP after immersion was measured, and the color difference Δb of the HAP surface before and after immersion was calculated. In the test solution that showed a lower color difference Δb than the control solution, it was determined that stain adhesion was suppressed. The coloring test was performed twice, and the average color difference Δb was calculated.

The color of the HAP was measured using the above ^- mentioned spectrophotometer under reflection measurement conditions and ^a measurement diameter of φ6 ^mm on the L ^* axis (lightness), a ^* axis (chroma: red/green axis), and b ^* axis (chroma: yellow/blue axis) of the L*a*b* color system based on JIS Z8722: 2009. When the colorimetric values of the HAP before immersion were L1, a1, and b1, and the colorimetric values of the HAP after immersion were L2, a2, and b2, the color difference Δb was calculated using the following formula.
Δb=b2−b1

(4) Results The results are shown in Table 1.

The results in Table 1 confirm that three types of collagen (Iquos HDL-50DR, Super Collagen Peptide SCP-2000, Water-soluble Collagen Peptide SS) and two types of dairy proteins (high whey protein WPI895, sodium caseinate) each suppress the deposition of stains caused by tobacco.

Example 2: Stain adhesion suppression effect of protein (2)
(1) Preparation of Protein Solution A protein solution was prepared according to the procedure described in Example 1(2), except that lysozyme described in Example 1(2) was replaced with lactoferrin (manufactured by Wako Pure Chemical Industries, Ltd.).

(2) Coloration test A commercially available medium-thick sauce (Bulldog medium-thick sauce, Bulldog Sauce Co., Ltd.) was mixed with each of the protein solutions obtained in (1) above at a mass ratio of 1:1 to prepare each test solution. A control solution was prepared by mixing the commercially available medium-thick sauce with water at a mass ratio of 1:1. The coloration test using each test solution was carried out according to the procedure described in Example 1 (3).

(3) Results The results are shown in Table 2.

The results in Table 2 confirm that three types of collagen (Iquos HDL-50DR, Super Collagen Peptide SCP-2000, Water-soluble Collagen Peptide SS) and two types of dairy proteins (high whey protein WPI895, sodium caseinate) each suppress staining caused by medium-thick sauce.

Example 3: Stain adhesion suppression effect by protein (3)
(1) Preparation of Curcumin Solution A curcumin solution was prepared by diluting 750 mg of 20% curcumin aqueous dispersion (manufactured by Yokohama Yushi Kogyo Co., Ltd.) to 100 mL.

(2) Preparation of Protein Solution The protein solution was prepared according to the procedure described in Example 1(2), except that the milk protein was replaced with two types of milk proteins (high whey protein WPI895 (Fonterra) and lactoferrin (Wako Pure Chemical Industries, Ltd.)).

(3) Coloration test Each test solution was prepared by mixing the curcumin solution obtained in (1) above with each protein solution obtained in (2) above at a mass ratio of 1:1. A control solution was prepared by mixing the curcumin solution obtained in (1) above with water at a mass ratio of 1:1. Coloration tests using each test solution were carried out according to the procedure described in Example 1 (3).

(4) Results The results are shown in Table 3.

The results in Table 3 confirm that three types of collagen (Iquos HDL-50DR, Super Collagen Peptide SCP-2000, Water-soluble Collagen Peptide SS) and milk protein (high whey protein WPI895) each inhibit the adhesion of stains caused by curcumin.

Claims (4)

A beverage for inhibiting stain adhesion (excluding beverages containing lactam compounds and beverages containing milk ) containing one or more components selected from the group consisting of collagen, casein and whey (excluding lactoferrin) , wherein the molecular weight of the collagen is a weight-average molecular weight of 500 to 6600, the casein content in the beverage is 0.2 mass% or less, and the beverage is a tea beverage or a coffee beverage. The beverage according to claim 1, wherein the staining is caused by smoking tobacco or eating staining foods. The beverage according to claim 2, wherein the tea beverage is a beverage selected from the group consisting of black tea beverages, oolong tea beverages, green tea beverages, cereal tea beverages, and rooibos tea beverages, as well as blends thereof. A method for producing a beverage for inhibiting stain adhesion (excluding beverages containing lactam compounds and beverages containing milk), which comprises blending one or more components selected from the group consisting of collagen, casein and whey (excluding lactoferrin) , wherein the molecular weight of the collagen is a weight-average molecular weight of 500 to 6600, the casein content in the beverage is 0.2 mass% or less, and the beverage is a tea beverage or a coffee beverage.