JP7285504B2 - Scalp condition improving agent and cosmetic containing the same - Google Patents

Description

Application of Article 30, Paragraph 2 of the Patent Act October 27, 2020, Nutrients 2020, 12 (11) 3285. doi: 10.3390/nu12113285

The present invention contains (A) at least one selected from glycerin monoalkyl ethers having a linear or branched alkyl group with 6 to 12 carbon atoms, and (B) naringenin, naringin, hesperidin and compounds thereof. A novel scalp condition improving agent containing a tight junction (TJ) formation promoter which is at least one selected from plant extracts, and a scalp cosmetic containing the scalp condition improving agent.

The skin is composed of the epidermis and the dermis, and the epidermis is further divided into the basal layer, spinous layer, granular layer, and stratum corneum from the deep layer. One of the important functions of the skin is the barrier function, which plays a role of protecting against invasion and attack of foreign substances from the outside world and a role of preventing transpiration of water from the body.
When this barrier function breaks down, foreign substances such as bacteria and viruses can easily enter the body, causing inflammation and skin diseases. In addition, it may cause evaporation of water from the body, causing dryness of the skin and disturbance of turnover.
Tight junctions (hereinafter referred to as "TJs") are intercellular adhesion apparatus present in the granular layer and play an important role in skin barrier function.

In recent years, from this point of view, attempts have been made to search for substances that control the formation of TJs (Patent Document 1). Studies have been conducted using plant-derived extracts as substances that control the formation of TJs. Patent document 3) has reported.
As with normal skin, it is important to improve the skin barrier function of the scalp as well. A lot of sebaceous glands and sweat glands exist in the scalp, and it is a part containing more water and oil than normal skin. The scalp has a lower barrier function and moisturizing effect than normal skin, and is prone to problems.
However, conventional TJ formation promoters focus on functioning in normal skin, with little reference to the scalp. There is a demand for a scalp condition-improving agent that is suitable for the environment of the scalp, excels in improving the skin barrier function of the scalp, and has a TJ formation-promoting action that enhances the effects of functional ingredients to be combined.

Japanese Unexamined Patent Application Publication No. 2016-113368 JP 2016-222558 A JP 2020-075877 A

An object of the present invention is to provide a scalp condition improving agent that is excellent in improving the skin barrier function of the scalp, and a scalp cosmetic using the same.

In order to solve the above problems, the present inventors have made extensive research efforts on the effects of flavonoids contained in plant extracts on TJ.
As a result, it was found that naringenin, naringin, and hesperidin exhibit excellent TJ formation-promoting activity, and plant extracts, particularly hesperetin, exhibit TJ-mitigating activity.
The present inventors have found a scalp condition improving agent containing a TJ formation accelerator by combining a glycerin monoalkyl ether having a linear or branched alkyl group of 6 to 12 carbon atoms with the above compound exhibiting a TJ formation accelerator. It has been found that it improves the familiarity with the scalp and improves the TJ formation promoting action on the scalp.
When the plant extract contained in the scalp condition improving agent contains both a compound exhibiting a TJ formation promoting effect and a compound exhibiting a TJ alleviating effect, the TJ alleviating effect necessary to effectively exhibit the TJ formation promoting effect. To the total amount of the compounds exhibiting the TJ formation promoting effect of the total amount of the compounds exhibiting the upper limit ratio to the total amount of compounds, the present invention is achieved.

Further, when a cosmetic containing a TJ alleviating agent and a functional ingredient is used as the first agent and a scalp cosmetic containing a scalp condition improving agent is used as the second agent, the TJ alleviating agent functions as the first agent. It is possible to promote the penetration of the functional ingredient by using it in combination with the functional ingredient, and to enhance the effect of the functional ingredient. The present invention was completed by discovering that the sustainability of the ingredients is improved.

That is, the present invention provides at least one selected from glycerin monoalkyl ethers having a linear or branched alkyl group having 6 to 12 carbon atoms, naringenin, naringin, hesperidin, and plant extracts containing these compounds. A scalp condition-improving agent containing at least one selected TJ formation promoter and optionally at least one TJ-relieving agent selected from hesperetin, quercetin, baicalin, and baicalein, and the scalp condition To provide a one-agent type scalp cosmetic containing an improving agent.
The present invention is characterized in that a scalp cosmetic containing a TJ alleviating agent and a functional ingredient is used as the first agent, and a scalp cosmetic containing the scalp condition improving agent is used as the second agent. To provide a two-part scalp cosmetic.

The present invention is characterized by the following points.
1. Contains at least one selected from glycerin monoalkyl ethers having a linear or branched alkyl group of 6 to 12 carbon atoms as component (A), and naringenin, naringin, hesperidin and their compounds as component (B). and a tight junction formation accelerator which is at least one kind selected from plant extracts.
2. Furthermore, as a component (C), a scalp condition improving agent containing at least one tight junction relaxation agent selected from hesperetin, quercetin, baicalin, and baicalein, wherein the naringenin contained in the above component (B). 2. The scalp condition-improving agent according to 1 above, wherein the total amount of component (C) is 25% by mass or less with respect to the total amount of , naringin and hesperidin.
3. 3. A scalp cosmetic containing the scalp condition improving agent according to 1 or 2 above.
4. A scalp cosmetic containing a tight junction mitigating agent [component (D)] and a functional component [component (E)] is used as the first agent, and the scalp cosmetic described in 3 above is used as the second agent. A two-component scalp cosmetic.
5. 4. A method for using a two-component scalp cosmetic, comprising applying the first component described in 4 above, and then applying the second component described in 4 above.

The scalp condition improving agent of the present invention can promote the formation of TJs and improve the barrier function of the skin, so it can protect the scalp from external stimuli and prevent evaporation of water and components in the body. Moreover, by blending the scalp condition improving agent, it is possible to provide a scalp cosmetic having an excellent skin conditioning effect.
In addition, the scalp cosmetic of the present invention can be used as a two-part scalp cosmetic. A scalp cosmetic containing a TJ alleviating agent and a functional ingredient is used as the first agent, and after the functional ingredient is deeply penetrated by the TJ alleviating agent, the scalp contains a scalp condition improving agent as the second agent. By using cosmetics for hair, it is possible to restore the barrier function of the scalp at an early stage, protect the scalp from external stimuli, and prevent evaporation of moisture and ingredients. By using it as a two-agent scalp cosmetic, it is possible to provide a two-agent scalp cosmetic having an excellent penetration effect.

A photograph of the condition of the scalp of a test subject observed under a microscope before the start of the test. A photograph of the condition of the scalp of a test subject observed under a microscope, taken after the end of the test. A photograph of the condition of the scalp of a test subject observed under a microscope, taken after the end of the test. A photograph of the condition of the scalp of a test subject observed under a microscope, taken after the end of the test. Figure 1 shows the condition of the test subject's scalp by keratin staining before the start of the test. The figure shows the condition of the test subject's scalp by keratin staining after the end of the test. The figure shows the condition of the test subject's scalp by keratin staining after the end of the test. The figure shows the condition of the test subject's scalp by keratin staining after the end of the test.

The scalp condition improving agent of the present invention comprises (A) at least one glycerin monoalkyl ether having a linear or branched alkyl group of 6 to 12 carbon atoms, and (B) naringenin, naringin, hesperidin and these and a tight junction formation accelerator which is at least one kind selected from plant extracts containing the compound of (1).
Since hesperetin, quercetin, baicalin, and baicalein exhibit a TJ-relieving action, hesperetin, quercetin, baicalin, and baicalein exhibit a TJ-relieving action relative to the total amount of the TJ formation promoters naringenin, naringin, and hesperidin. The total amount of baicalein is preferably 25% by mass or less.

(A) Component In the present invention, the (A) component exhibits the effect of improving the familiarity with the scalp and improving the effect of promoting the formation of tight junctions on the scalp.
(A) Glycerin monoalkyl ether having a linear or branched alkyl group having 6 to 12 carbon atoms in the present invention is produced from glycerin and a monohydric alcohol having a linear or branched alkyl group having 6 to 12 carbon atoms. Also, commercially available products such as Glymoist (manufactured by NOF Corporation), Adekanol NHG (manufactured by ADEKA Corporation), 3-((2-Propylheptyl)Oxy) Propane-1, A reagent such as 2-Diol (manufactured by Chemieliva Pharmaceutical Co. Ltd.) may be used.
(A) component may use only 1 type and may use 2 or more types together. From the viewpoint of compatibility with the scalp, the alkyl group of glycerin monoalkyl ether is preferably a straight or branched alkyl group having 7 to 9 carbon atoms, more preferably a branched alkyl group having 7 to 9 carbon atoms.
Examples of the glycerin monoalkyl ether include 3-(2-ethylhexyloxy)-1,2-propanediol (hereinafter referred to as "ethylhexylglycerin"), 3-(2-ethylheptyloxy)-1,2- and propanediol. Among them, ethylhexylglycerin is even more preferable from the viewpoint of good compatibility with the scalp.
The total content of component (A) in the scalp condition improving agent of the present invention is preferably 0.001 to 10% by mass. If the total content is less than the above lower limit, the effect of the present invention may not be obtained, and if the above upper limit is exceeded, there is a tendency that the effect commensurate with the content cannot be obtained, which is disadvantageous in terms of cost.

(B) Component: TJ Formation Promoter Naringenin, naringin, and hesperidin, which are components (B) of the present invention, may be compounds or plant extracts containing these compounds. .
Further, these compounds may be transglycosylated forms for the purpose of improving dispersibility and solubility. (e.g. glycosyltransferase hesperidin)
Plant extracts include, for example, plant extracts obtained from citrus fruits such as pomelo, grapefruit, orange, bitter orange, E-chan lemon, hassaku, tachibana, yuzu, bellows, and mandarin orange.
All of the above plant extracts are commonly used as ingredients of pharmaceuticals, folk medicines, foods, and cosmetics. Among them, extracts of pomelo, grapefruit, mandarin orange, orange, bellows, E-chan lemon, and bitter orange are preferred, and extracts of pomelo, grapefruit, and mandarin orange are more preferred from the viewpoint of their high content of naringenin, naringin, and hesperidin. .

The plant extract in the present invention may be one extracted by a conventional extraction method, or a commercially available one. The extraction method is not particularly limited, and a general extraction method using a solvent can be used. The plant used for extraction may be used as it is, or may be used after being pulverized or dried, but it is preferably used after being pulverized, and more preferably after being dried.
Solvents used for extraction are not particularly limited, and include water, lower monohydric alcohols (eg, methanol, ethanol, propanol, butanol, etc.), polyhydric alcohols (eg, propylene glycol, dipropylene glycol, butylene glycol, glycerin, etc.). , esters (e.g. methyl acetate, ethyl acetate, etc.), ketones (e.g. acetone, methyl ethyl ketone, etc.), ethers (diethyl ether, tetrahydrofuran, etc.) and other polar solvents, and halogenated hydrocarbons (e.g. chloroform, carbon tetrachloride etc.), hydrocarbons (eg, hexane, heptane, cyclohexane, etc.) and the like can be used.
One of these may be used alone, or a mixed solvent comprising two or more thereof may be used. In particular, it is preferable to use a polar solvent, and it is more preferable to use methanol.
The extraction procedure is not particularly limited, and includes, for example, a procedure of immersing the plant used for extraction in the solvent at 20 to 40° C. for 1 hour to 7 days and filtering.
Further, by diluting, concentrating, freeze-drying, and purifying the obtained extract, it is possible to obtain a diluted solution, a concentrated solution, a dried product, and a purified product of the extract, and these are used as the plant extract according to the present invention. can also be used.

In the present invention, component (B) can be at least one selected from naringenin, naringin, hesperidin, and plant extracts containing these compounds.
The total amount of naringenin, naringin and hesperidin contained in component (B) in the scalp condition improving agent of the present invention is preferably 0.000001 to 10% by mass in terms of solid matter. If the total amount is less than the lower limit, the effect of the present invention may not be obtained.

Component (C): TJ alleviating agent Component (C) of the present invention includes hesperetin, quercetin, baicalin, and baicalein contained in plant extracts when plant extracts are used as component (B). .
When a plant extract is used as the TJ formation promoter of component (B), the total amount of the TJ formation promoters naringenin, naringin, and hesperidin is reduced by the amount of component (C), namely hesperetin, which is a TJ relaxation agent. , quercetin, baicalin, and baicalein in a total amount of more than 25%, the effect of promoting TJ formation tends to deteriorate.
That is, the following conditions are the conditions for effectively exhibiting the TJ formation promotion effect.
Total amount of TJ relaxation agent compound/Total amount of TJ formation accelerator compound ≤ 0.25

In addition, the definition of each symbol in the above is as follows.
Total amount of TJ relaxant compound:
Total amount of compounds showing TJ alleviating action contained in the first agent Total amount of TJ formation promoter compounds:
Total amount of compounds exhibiting TJ formation promoting action contained in the first agent

The scalp condition-improving agent of the present invention can be incorporated into various external preparations such as pharmaceuticals, quasi-drugs, medicinal or cosmetic preparations, and is excellent due to its excellent TJ formation promoting effect. It is suitable to be blended in a scalp cosmetic to be applied to the scalp, since it has a skin-conditioning effect.
When applied to the scalp, the scalp cosmetic of the present invention can promote the formation of TJs in the scalp and improve the barrier function of the scalp.

As described above, the scalp condition-improving agent of the present invention can be used in a one-agent type scalp cosmetic by containing component (A), component (B), and component (C).
The scalp condition improving agent of the present invention can also be used as the second agent of a two-agent scalp cosmetic.
A scalp cosmetic containing a TJ alleviating agent [(D) component] and a functional component [(E) component] is used as the first agent, and a scalp cosmetic containing a scalp condition improving agent is used as the second agent. By using it, it is possible to provide a two-component scalp cosmetic excellent in skin conditioning action and promotion of permeation of functional ingredients.
That is, by applying the first agent of the two-agent scalp cosmetic, the TJ is relieved and the penetration of the functional ingredient is promoted, and then the loosened TJ is removed by applying the second agent of the two-agent scalp cosmetic. It can restore (promote TJ formation) and improve the durability and barrier function of functional ingredients.

(D) Component: TJ alleviating agent The (D) component of the present invention is a component contained in the first agent of a two-part scalp cosmetic, and is not particularly limited as a TJ alleviating agent, and is a conventionally known A TJ-relieving agent can be used, and examples thereof include hesperetin, quercetin, baicalin, baicalein, and compounds thereof, which are the same compounds as component (C).
Indomethacin, diclofenac, and the like, which are conventionally known TJ-relieving agents, can also be used.
Among them, it is preferable to use baicalein because of its excellent TJ relaxation action.
The above baicalein may be a single compound or an extract. Examples of the extract include Scutellaria root extract, which is a crude drug obtained by removing the pericarp from the root of Scutellaria baicalen of the Labiatae family and drying it.

The total amount of each of the TJ formation promoter and the TJ alleviating agent of the present invention contained in the two-agent scalp cosmetic of the present invention can be appropriately adjusted depending on the dosage form of the two-agent scalp cosmetic. It is preferably 0.000001 to 10% by mass in terms of solid matter. If the total amount is less than the above lower limit, the effect of the present invention may not be obtained, and if the above upper limit is exceeded, there is a tendency that the effect commensurate with the amount cannot be obtained, which is disadvantageous in terms of cost.

(E) Component: Functional component The functional component is not particularly limited, and components that exhibit useful effects on the skin can be used. Examples include components exhibiting effects such as anti-inflammatory action, Maillard reaction inhibitory action, pigmentation inhibitory action, anti-wrinkle action, skin repair activity, elastase activity inhibitory action, lipase inhibitory action, and tyrosinase inhibitory action.
For example, ingredients that exhibit moisturizing action include ceramide, hyaluronic acid, collagen, polysaccharides, glycerin, urea, and amino acids (see Patent No. 5932948); ingredients that exhibit hair growth action include minoxidil, adenosine, and coleus. Forskohlii root extract, Astragalus root extract, Chimp extract (see Patent No. 5932948, Japanese Patent Application Laid-Open No. 2019-178099). be done.
Ingredients that exhibit antioxidant activity include dibutylhydroxytoluene (BHT), dibutylhydroxyanisole (BHA), and tocopherol acetate (see Patent No. 5932948). See Japanese Patent No. 5932948.), and examples of components exhibiting a Maillard reaction inhibitory action include karkade extracts, hibiscus extracts, and tonin extracts (see Japanese Patent No. 5300233).

The scalp condition improving agent of the present invention uses a scalp cosmetic containing a TJ alleviating agent (D) component and a functional component (E) as the first agent, and contains a scalp condition improving agent as the second agent. It can be used as a two-part scalp cosmetic using a scalp cosmetic, and the application of the first part relieves TJ and promotes the penetration of the functional ingredients, and then the application of the second part loosens it. It can restore TJ and improve the durability and barrier function of functional ingredients.
When the first agent contains the TJ formation accelerator as the component (B), it is necessary to satisfy the following conditions.
Preferably, the ratio of the total amount (mass) of the TJ relaxation agent compound to the total amount of the TJ formation accelerator compound is 0.25 or more, more preferably, the ratio of the total amount of the TJ relaxation agent compound to the total amount of the TJ formation accelerator compound is , 0.5 or more, and more preferably, the ratio of the total amount of TJ-relieving compounds to the total amount of TJ formation-promoting compounds is 1.0 or more.

In addition, the definition of each symbol in the above is as follows.
Total amount of TJ relaxant compound:
Total amount of compounds exhibiting TJ alleviating action contained in the first agent Total amount of TJ formation promoting compounds:
Total amount of compounds that promote TJ formation contained in the first agent

(F) Optional Components The scalp cosmetic of the present invention may contain, in addition to the essential components described above, optional components used in ordinary scalp cosmetics within a range that does not interfere with the present invention. . Examples of such optional components include excipients, thickeners, solvents, dispersants, solubilizers, buffers, preservatives, antioxidants, colorants and the like.
Specifically, water; excipients such as lactose and sucrose; thickeners such as natural gums, cellulose derivatives and acrylic acid polymers; solvents such as alcohols, glycols, animal and vegetable oils and fats; Dispersants such as monooleate, polyoxyethylene hydrogenated castor oil, and polyethylene glycol; Solubility aids such as D-mannitol and benzyl benzoate; Buffers such as phosphates, acetates and carbonates; Paraoxybenzoic acid esters , chlorobutanol, benzyl alcohol, and other antiseptics; sulfites, ascorbic acid, and other antioxidants;
Water, alcohols, and polyoxyethylene hydrogenated castor oil are particularly preferred, and water, ethanol, butylene glycol, and PEG30 hydrogenated castor oil are more preferred.

The cosmetic for scalp of the present invention can also contain ingredients conventionally used in cosmetic for scalp to the extent that the effects of the present invention are not impaired. Such ingredients include, for example, moisturizing agents, emollients, blood circulation promoters, cell activators, antioxidants, anti-inflammatory agents, antibacterial agents, peroxide inhibitors, hair restorers, ingredients having hair nourishing effects, Examples include various extracts derived from plants, animals, and microorganisms other than those mentioned above.
Specific examples of ingredients that can be added include moisturizing agents such as glycerin, urea, amino acids, and hyaluronic acids; emollients such as squalane, macadamia nut oil, olive oil, jojoba oil, and silicon oil; blood circulation promoters such as vitamin E and capsicum tincture. Agents; cell activators such as nucleic acids, antioxidants such as dibutylhydroxytoluene (BHT), dibutylhydroxyanisole (BHA), and tocopherol acetate; anti-inflammatory agents such as glycyrrhizin and allantoin; hinokitiol, benzalkonium chloride, chlorhexidine salt , antibacterial agents such as parahydroxybenzoic acid esters; superoxide dismutase (SOD) and other peroxide inhibitors; Ingredients having hair-restoring and hair-nourishing effects, such as compounds such as forskolin; various extracts derived from plants, animals, and microorganisms other than the above, such as ginkgo biloba extract, placenta extract, and lactic acid bacteria culture extract;
The total content of the above components in the scalp cosmetic of the present invention is not particularly limited, but when formulating, it can be appropriately selected depending on the dosage form, the number of times of use, the type of excipients and additives to be blended, etc. .
In addition, the hair cosmetic composition of the present invention may contain base components such as surfactants and oils, thickeners, preservatives, tonicity agents, and antioxidants within the range that does not impair the effects of the present invention. , ultraviolet absorbers, chelating agents, perfumes, coloring agents, and the like.

The scalp cosmetics of the present invention include, for example, essences, shampoos, hair treatments, lotions, milky lotions, and creams.
When using a one-agent type scalp cosmetic, apply the scalp cosmetic several times a day (preferably twice a day), 0.5 ml to 5 ml each time, to the entire scalp and rub it in.
When using a two-agent scalp cosmetic, apply the first agent and the second agent once a day, 0.5 to 5 ml each time, to the entire scalp and rub it in. In this case, it is preferable to apply the second agent 3 to 12 hours after the application of the first agent. More preferably, a significant effect can be expected by applying the first agent before going to bed and the second agent after waking up.

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited to these Examples.

(1) Extract sample Fruit was divided into pulp and pericarp (exocarp or mesocarp), and the pericarp was used.
The pericarp was dried overnight at 60° C. using a constant temperature and humidity chamber (DK600, manufactured by Yamato Scientific Co., Ltd.) and pulverized using the juicer-mixer. A 100 mg milled sample was taken in a capped test tube, added with 5 ml of methanol/dimethylsulfoxide extraction solvent (volume ratio 1/1), and extracted at room temperature for 12 hours with reciprocating shaking. After centrifuging the obtained extract (3,000 rpm×10 min) with the hybrid high-speed refrigerated centrifuge, the supernatant was collected and used as an extract sample.

(2) Analysis sample manufacturing method The extract sample obtained from the pericarp is diluted twice with an eluent of acetonitrile/acetic acid aqueous solution (volume ratio 5/95), and 1 ml of the diluted solution is filtered through a 0.45 μm membrane filter (( (manufactured by Shimadzu GLC) to obtain an analysis sample.

(3) Analysis conditions Analysis was performed by high performance liquid chromatography (HPLC measurement).
Apparatus used: UltiMate 3000 (manufactured by Thermo Fisher Scientific)
Column: TSKgel ODS-100S (C18, particle size 5 μm, 4.6 mm ID × 25 cm) (manufactured by Tosoh Corporation)
Conditions: column temperature 40° C., acetonitrile/0.1 mass % acetic acid aqueous solution (20/80→40/60:0 min→20 min) eluent, flow rate 1 ml/min, detector UV=254, 280 nm
Standard products: Naringin (manufactured by Sigma-Aldrich Japan GK), Hesperidin (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.), Naringenin (manufactured by Tokyo Chemical Industry Co., Ltd.), Hesperetin (manufactured by Tokyo Chemical Industry Co., Ltd.) ), narirutin (manufactured by FUJIFILM Wako Pure Chemical Industries, Ltd.)
(Raw material production example 1) Grapefruit extract An extract sample was collected using grapefruit pericarp. HPLC analysis of the extract sample confirmed the content of 0.14% by weight of naringin (71% by weight of the flavanones detected) and no naringenin, hesperidin and hesperetin.
(Raw material production example 2) Mandarin orange extract An extract sample was recovered using mandarin orange peel. HPLC analysis of an extract sample confirmed the content of 0.04% by weight (30% by weight of flavanones detected) hesperidin and 0.07% by weight (50% by weight of flavanones detected) narirutin. However, naringenin, naringin, and hesperetin were not identified.
(Raw material production example 2) Pomelo extract An extract sample was recovered using the pomelo skin. HPLC analysis of the extract sample confirmed the content of 0.13% by weight (70% by weight of the flavanones detected) of naringin and no naringenin, hesperidin and hesperetin.

(4) Skin conditioning effect test of scalp condition improving agent As the scalp condition improving agent of the present invention, PEG-30 hydrogenated castor oil (NIKKOL HCO-30, manufactured by Nikko Chemicals Co., Ltd.), ethanol (ethyl alcohol 99 degrees anhydrous) Grade, manufactured by Mitsubishi Chemical Corporation), 1,3-butylene glycol (High Sugar Cane BG, manufactured by KOKYU ALCOHOL KOGYO Co., Ltd.), Ethylhexylglycerin (Glymoist, manufactured by NOF Corporation), Naringin (Sigma Aldrich Japan GK), naringenin (manufactured by Tokyo Chemical Industry Co., Ltd.), hesperidin (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.), hesperetin (manufactured by Tokyo Chemical Industry Co., Ltd.), grapefruit extract, The mandarin orange extract and the pomelo extract were blended into scalp cosmetics, and their skin conditioning effects were evaluated.

(4-1) Preparation of Scalp Cosmetic A test scalp cosmetic was prepared with the composition shown in Table 1 based on the composition shown in Table 2. The unit of the blending amount is parts by mass, and the "remaining amount" of purified water is the amount required to make the total amount 100 parts by mass.

(4-2) Evaluation For the scalp cosmetics of Examples 1 to 11 and Comparative Examples 1 to 3, 7 groups of 6 men and women aged 25 to 40 were used as subjects.
The test subject applied about 5 pushes (about 1.3 mL) of the scalp cosmetic in a spray bottle to the entire scalp every day before going to bed and after waking up, and then massaged and kneaded the scalp. This monitoring test was continued for 4 weeks, and before the start of the test and 4 weeks after the end of the test, the moisture content of the scalp was measured, the scalp condition was observed with a microscope, and the scalp environment was evaluated by keratin staining. Each was evaluated according to the following evaluation criteria (see (6-1), (6-2) and (6-3) below).
During this period, when scalp cosmetics other than those described above were used, the ones normally used were used as they were. None of the subjects complained of skin or physical abnormalities during the period of use.
As is clear from the above results, it was confirmed that the TJ formation promoter of the present invention exerts a remarkable skin-conditioning action by blending it in a scalp cosmetic.

(5) Skin-conditioning effect test of a two-agent scalp cosmetic composition The two-agent scalp cosmetic composition of the present invention contains scutellaria root extract as a TJ alleviating agent of the first agent and hydrolyzed collagen or hydrolyzed elastin as a functional ingredient. A grapefruit extract was blended as a TJ formation accelerator of the second agent, and the skin conditioning action was evaluated.

(5-1) Preparation of two-agent type scalp cosmetic composition Essence as a two-agent type scalp cosmetic for testing, based on the composition shown in Table 4, the first agent (formulation example 1-3) with the composition shown in Table 3 ) and the second agent (formulation example 1-3) were prepared respectively. The unit of the blending amount is parts by mass, and the "remaining amount" of purified water is the amount required to make the total amount 100 parts by mass.

(5-2) Evaluation For the two-agent type scalp cosmetics of Examples 12-13 and Comparative Examples 4-9, 7 groups of 6 men and women aged 25-40 were used as subjects.
The test subject applied a two-agent scalp cosmetic in a spray bottle every day, applying the first agent before going to bed and the second agent after waking up, applying about 5 pushes (about 1.3 mL) to the entire scalp each time. I massaged and kneaded. This monitoring test was continued for 4 weeks, and before the start of the test and 4 weeks after the end of the test, the moisture content of the scalp was measured, the scalp was observed with a microscope, and the scalp environment was evaluated by keratin staining. Each was evaluated according to the following evaluation criteria (see (6-1), (6-2) and (6-3) below).
During this period, when scalp and hair cosmetics other than those described above were used, the ones normally used were used as they were. None of the subjects complained of skin or physical abnormalities during the period of use.

As is clear from the above results, the two-agent scalp cosmetic of the present invention has remarkable functionality by blending a TJ alleviating agent and a functional ingredient in the first agent and a TJ formation promoter in the second agent. It was confirmed that it has an effect of penetrating the ingredients and exerts a skin conditioning effect.

(6-1) Measurement of stratum corneum moisture content of the scalp The stratum corneum moisture content of the scalp of the test subjects was measured with a high-sensitivity stratum corneum thickness/moisture meter (ASA-MX3, manufactured by Japan Ash Co., Ltd.). The area of 5 cm above the ear of the test subject was measured five times, and the average value was calculated. The difference obtained by subtracting the stratum corneum moisture content before the start of the test from the moisture content of the stratum corneum after the test was evaluated according to the following criteria.
5: The difference in moisture content is +15% or more 4: The difference in moisture content is +5% or more and less than +15% 3: The difference in moisture content is less than ±5% 2: The difference in moisture content is -5% More than -15% or less 1: The difference in moisture content is -15% or more

(6-2) Observation of Scalp Condition with Microscope The scalp condition of the test subjects was observed with a microscope (i-Scope USB 2.0, manufactured by Moritex Co., Ltd.). The condition of the scalp after the end of the test was compared with that before the end of the test and evaluated according to the following criteria.
5: Pore clogging is considerably improved and the texture is considerably improved 4: Pore clogging is slightly improved and the texture is slightly improved 3: No change 2: Pore clogging is slightly worsened and the texture is slightly improved Disturbed 1: The clogging of pores is considerably worsened, and the texture is considerably disturbed. The change in Fig. 1 to Fig. 2 is evaluated as 3, the change in Fig. 1 to 3 is evaluated as 4, and the change in Fig. 1 to 4 is evaluated. was rated as 5.

(6-3) Evaluation of scalp condition by keratin staining The scalp condition of the test subject was evaluated by keratin staining. The stratum corneum cells were peeled off from the scalp at 5 cm above the ear of the test subject by tape stripping, stained with an aqueous solution of gentian violet, and observed. The condition of the scalp after the end of the test was compared with that before the start of the test and evaluated according to the following criteria.
5: The stratum corneum detachment was considerably reduced, and the shape of the majority of the stratum corneum cells was five to hexagonal. 3: No change 2: Slightly increased stratum corneum detachment, some of the stratum corneum cells are deformed 1: Considerably increased stratum corneum detachment, most of the stratum corneum cells are deformed From Fig. 5 The change in FIG. 6 was rated 3, the change from FIG. 5 to FIG. 7 was rated 4, and the change from FIG. 5 to FIG. 8 was rated 5.

Using the average score of test subjects in each example and comparative example for each test, it was classified as follows.
<Evaluation judgment>
◎: Very good (the average score of 6 people was 4 points or more)
○: Good (The average score of 6 people was 3 points or more and less than 4 points)
×: Bad (The average score of 6 people was less than 3 points)

INDUSTRIAL APPLICABILITY The scalp condition improving agent of the present invention has an excellent barrier function strengthening action and skin conditioning action, and can be used in various external preparations including cosmetics for the purpose of preventing or improving rough skin.
In addition, the hair cosmetic composition of the present invention has an excellent skin-conditioning action, and can prevent and improve rough skin.

Claims (2)

A first component is a scalp cosmetic containing a functional ingredient and a tight junction relaxation agent that is at least one selected from hesperetin, quercetin, baicalin, and baicalein, and component (A) has 6 to 12 carbon atoms. and at least one selected from naringenin, naringin, hesperidin and plant extracts containing these compounds as component (B). A two-agent type scalp cosmetic, wherein a scalp cosmetic containing a tight junction formation accelerator is used as the second agent. 2. The two-agent scalp cosmetic composition according to claim 1 , wherein the first agent is applied and then the second agent is applied.

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