JP6717482B2 - Blood collection tube - Google Patents

Description

The present invention relates to blood collection tubes.

When collecting blood in a laboratory in a blood test, a method using a syringe or a vacuum blood collection tube is used, but in recent years, blood collection using a vacuum blood collection tube has been increasing. The vacuum blood collection tube contains a reagent depending on the purpose of the test such as blood coagulation test and platelet test, and a vacuum blood collection tube suitable for the test is used. A vacuum blood collection tube with a volume of about 2-5 mL is mainly used, but the amount of blood used for the test is reduced due to the progress of the test technology, and a vacuum blood collection tube for collecting a smaller amount of blood. Are known (for example, see Patent Document 1).

JP, 2007-167124, A

When using a vacuum blood collection tube having a volume of 2 mL or more, more blood may be collected than the amount used for the test. Further, in order to reduce the volume of the vacuum blood collection tube, it is necessary to prepare a vacuum blood collection tube and a holder having a size corresponding to the blood collection amount. Furthermore, it is difficult to maintain a constant blood collection amount with a vacuum blood collection tube having a volume of 1 mL or less. Therefore, the vacuum blood collection tube may be difficult to use in the examination of the pediatric region and the veterinary region such as small dogs.

Further, the vacuum blood collection tube is convenient when directly collecting blood by puncturing a needle in an examination room, but when a blood collection catheter (A-line or the like) is secured as in the case of surgery, the vacuum blood collection tube is used. It is possible to collect the amount of blood used for the test with a syringe from the catheter without puncturing the blood. Therefore, there has been a demand for a blood collection tube capable of storing a small amount of blood collected by a syringe.

Furthermore, a small amount of blood collected with a syringe is injected into a blood collection tube in which a reagent used for one test is sealed. The encapsulated reagent contains an anticoagulant such as sodium citrate, heparinase, and the like. Sodium citrate as an aqueous solution and heparinase in a freeze-dried state are sealed in a blood collection tube. Other reagents are enclosed by coating the inner surface of the blood collection tube. Therefore, the blood collection tube is required to be sealed before use so that the solution does not leak and that the freeze-dried reagent does not deteriorate due to external steam.

An object of the present invention is to provide a technique for injecting a small amount of blood from a syringe into a closed blood collection tube.

In order to solve the above problems, the present invention provides, in a connecting member that connects a syringe and a blood collection tube, a concave portion that reaches an end portion is formed in the circumferential direction on the inner peripheral surface of the tube portion that fits into the blood collection tube on the blood collection tube side. A sealing member having an internal valve is slid so as to be in sliding contact with a plurality of convex portions provided in the concave portion to form a blood flow path from the syringe to the blood collection tube.

More specifically, the blood collection tube according to the present invention is a blood collection tube into which blood collected by a syringe is injected, and one end of the tube-shaped connection part is connected to the tip of the syringe and the other end of the connection part. A plug part that closes the opening of the blood collection tube and is provided with a plurality of air holes that communicate with the outside of the blood collection tube, and a tubular part that communicates with the connecting part through the plug part and that fits into the opening of the blood collection tube. A connecting member having a recessed portion extending in the circumferential direction on the inner peripheral surface opposite to the plug portion and extending to the end portion, and a tubular portion provided with a plurality of axially formed convex portions on the inner peripheral surface of the recessed portion. And a seal part that is slidable inside the tubular part and that is in sliding contact with the convex part in the concave part, and an internal valve that is formed on the syringe side of the seal part and that has a plurality of notches and that is inserted into the connecting part. And a sealing member having the same.

With the above blood collection tube, even if it is a small amount of blood collected with a syringe, the opening of the blood collection tube is closed with a plug, and a desired amount of blood can be injected from the syringe through the connecting part and the tube You can For example, when a catheter for blood collection is secured, such as during surgery, it is possible to collect a small amount of blood and perform regular examinations. Further, the above blood collection tube can be used for children or animals having a limited blood volume. The notch of the internal valve is not limited to any number as long as it is formed so that blood injected from the connecting portion can flow into the tubular portion.

In addition, the seal part slides to a position in which the tip of the syringe fits into the connecting part from the side opposite to the internal valve and pushes the internal valve to slide contact with the convex part, and the connecting part, the notch, and the seal part. A flow path from the syringe to the blood collection tube may be formed through the opening formed by the outer circumference and the recess. With such a blood collection tube, by connecting the syringe to the blood collection tube, a blood flow path from the syringe to the blood collection tube is formed, so that blood can be easily injected from the syringe into the blood collection tube. is there.

Further, the blood collection tube may contain an anticoagulant. A blood collection tube containing an anticoagulant can suppress the progress of blood coagulation. The anticoagulant may be contained in a liquid, freeze-dried, or dry-coated state. If the blood collection tube is blocked by the plug portion as described above, the anticoagulant in the blood collection tube can be isolated from the outside air and the deterioration of the anticoagulant can be suppressed. Further, the anticoagulant may be included in the amount used for one or two measurements. Rapid measurement is possible without injecting an anticoagulant after injecting blood.

Anticoagulants contained in blood collection tubes include sodium citrate, EDTA, hirudin, BAPA (benzylsulfonyl-D-Arg-Pro-4-amidinobenzylamide), corn-derived trypsin inhibitor (1977. J. Biol. Chem. 252.8105), heparin, aprotinin and the like. With such a blood collection tube, it is possible to store the collected blood by a blood collection tube containing an anticoagulant suitable for the inspection item.

The blood collection tube may have a volume of 3 mL or less, preferably 1.5 mL or less. With such a blood collection tube, a blood collection amount of 3 mL or less, preferably 1.5 mL or less, can be tested, and thus can be used for children or animals.

Further, the blood collection tube may have a scale for measuring the internal volume. With such a blood collection tube, it becomes easy to inject the amount of blood used.

According to the present invention, a small amount of blood can be injected from a syringe into a closed blood collection tube.

FIG. 1 is a schematic diagram showing the configurations of a syringe and a blood collection tube. FIG. 2 is a first perspective view of the connecting member. FIG. 3 is a second perspective view of the connecting member. FIG. 4 is a perspective view showing a first example of the seal member. FIG. 5 is a perspective view showing a second example of the seal member. FIG. 6 is a diagram showing a state before and after the seal member slides inside the connecting member. FIG. 7 is a cross-sectional view showing a state in which the seal member is pushed in by the syringe inside the connecting member. FIG. 8 is a perspective view showing a state in which the seal member is pushed in by the syringe inside the connecting member.

Hereinafter, an embodiment of the present invention will be described with reference to the drawings. The configurations of the following embodiments are exemplifications, and the present invention is not limited to the configurations of the embodiments.

[Embodiment]
FIG. 1 is a schematic diagram showing the configurations of a syringe and a blood collection tube. The blood collection tube 10 includes a connecting member 11, a sealing member 12, and a tube 13. The blood collected by the syringe 20 is injected into the tube 13 via the connecting member 11 and the seal member 12.

The connecting member 11 is a tubular member, and one end thereof is connected to the syringe 20. In FIG. 1, the connecting member 11 is connected to the syringe 20 by screwing the tip portion of the syringe 20 into one end of the connecting member 11. At this time, the tubular portion 22B formed in the tubular opening in the tip portion of the syringe 20 screwed into the connecting member 11 is fitted into the connecting member 11.

The other end of the connecting member 11 is fitted into the tube 13, and the opening of the tube 13 is sealed by the seal member 12 fitted inside the connecting member 11. When the syringe 20 is screwed into the connecting member 11, the seal member 12 is pushed down, and a blood flow path from the syringe 20 to the tube 13 is formed.

The seal member 12 is slidable inside the portion where the connecting member 11 is fitted with the tube 13. On the inner peripheral surface of the connecting member 11 on the tube 13 side, a recess reaching the end is formed in the circumferential direction, and the seal member 12 is prevented from falling off by a plurality of protrusions provided in the recess. The gap formed between the outer peripheral surface of the seal member 12 and the inner peripheral surface of the connecting member 11 forms a blood flow path from the connecting member 11 to the tube 13. When blood flows from the connecting member 11 into the tube 13, the air in the tube 13 flows to the connecting member 11 side through a part of the blood flow path.

The tube 13 stores the blood injected from the syringe 20. The tube 13 may include an anticoagulant. By containing the anticoagulant, the tube 13 can suppress the progress of blood coagulation. The anticoagulant contained in the tube 13 is sodium citrate, EDTA, hirudin, BAPA (benzylsulfonyl-D-Arg-Pro-4-amidinobenzylamide), corn-derived trypsin inhibitor (1977. J. Biol. Chem. 252.8105), heparin, aprotinin and the like. It may also include heparinase. By changing the type of anticoagulant in the tube 13, blood can be stored using an anticoagulant suitable for the test item. After injecting blood into the tube 13, the tube 13 may be sealed by removing the connecting member 11 and closing the lid.

The tube 13 may have a volume of 3 mL or less, preferably 1.5 mL or less. By setting the volume to 3 mL or less, preferably 1.5 mL or less, the blood collection tube 10 including the tube 13 can be used for children, animals, and the like. The tube 13 may be provided with a scale line at a position indicating the amount of blood to be injected. By providing the scale line, it becomes easy to inject the used amount of blood.

The anticoagulant contained in the tube 13 may be contained in an amount used for one or two measurements. With the blood collection tube 10 including the tube 13 as described above, it is not necessary to inject a reagent (anticoagulant) after injecting blood, and thus a quick examination is possible.

The syringe 20 includes a piston 21 and a syringe body 22. The syringe 20 is connected to the connecting member 11 and presses the piston 21 to inject the collected blood into the tube 13. The syringe body 22 has a cylindrical opening 22A having a larger outer diameter than the sliding portion of the piston 21 at the tip portion. In the opening 22A, a tubular portion 22B that fits into the connecting member 11 is formed. The syringe body 22 forms a flow path of blood to the tube 13 via the tubular portion 22B fitted into the connecting member 11. That is, the blood in the syringe main body 22 does not flow into the tubular opening 22A (outside the tubular portion 22B fitted into the connecting member 11). The tubular portion 22B protrudes from the opening surface of the opening 22A, and when the tubular portion 22B is fitted into the connecting member 11, the sealing member 12 arranged inside the connecting member 11 is pushed toward the tube 13 side. The tip portion of the syringe 20 is not limited to the shape shown in FIG. 1 as long as the seal member 12 arranged in the connecting member 11 can be pushed into the tube 13 side by connecting with the connecting member. Good.

Hereinafter, details of the connecting member 11 and the seal member 12 will be described. FIG. 2 is a first perspective view of the connecting member. The connecting member 11 includes a connecting portion 11A, a plug portion 11B, and a tubular portion 11C. An air hole 11D is formed in the plug portion 11B.

The connecting portion 11A has a tubular shape so that blood can flow into the connecting portion 11A, and one end of the connecting portion 11A is screwed into the opening 22A of the tip portion of the syringe 20. As shown in FIG. 2, the syringe 20 and the connecting member 11 are connected by screw connection, but the connecting method is not limited to screw connection. When injecting blood into the blood collection tube 10 from the syringe 20, it is sufficient that the connecting member 11 does not fall off the tip portion of the syringe 20, and the syringe 20 and the connecting member 11 may be connected by, for example, fitting. ..

The plug portion 11B is formed at the other end of the connecting portion 11A so as to close the opening of the tube 13. The plug portion 11B is provided with an opening at a connecting portion with the connecting portion 11A so as to communicate with the inside of the connecting portion 11A. Further, the plug portion 11B has an air hole 11D for allowing the air inside the tube 13 to escape to the outside of the tube 13 when the blood is injected from the syringe 20 into the tube 13. Note that the number of the air holes 11D is not limited, and the number of the air holes 11D may be provided according to the injection amount or the injection speed of blood from the syringe 20 into the blood collection tube 10.

The tubular portion 11C is formed so as to be connected to the plug portion 11B and fits into the opening of the tube 13. The air hole 11D provided in the plug portion 11B communicates with the inside of the tube portion 11C and allows the air inside the tube 13 to escape to the outside of the tube 13.

FIG. 3 is a second perspective view of the connecting member 11. Similar to FIG. 2, the connecting member 11 includes a connecting portion 11A, a plug portion 11B, and a tubular portion 11C. Since the connecting portion 11A and the plug portion 11B are the same as those in FIG. 2, the description thereof will be omitted.

The cylindrical portion 11C has a concave portion 11C1 extending in the circumferential direction on the inner peripheral surface on the opening side opposite to the plug portion 11B and extending to the end portion. In addition, the concave portion 11C1 is provided with a plurality of convex portions 11C2 that are formed in the axial direction. In the example of FIG. 3, the protrusions 11C2 are provided at three locations, but the location is not limited to three. The flow passage area of the blood into the tube 13 formed by the outer periphery of the cylindrical portion 11C and the convex portion 11C2 is ensured so that the backflow of the blood to the air hole 11D of the plug portion 11B does not occur. I wish I had it. Other description of the tubular portion 11C is common to that of FIG.

FIG. 4 is a perspective view showing a first example of the seal member 12. The seal member 12 includes a seal portion 12A and an internal valve 12B.

The seal portion 12A is fitted inside the connecting member 11 and slides inside the connecting member 11. First, the seal portion 12A is arranged at a position where the surface contacts the stopper portion 11B. In this case, the internal valve 12B is in a state of being inserted into the connecting portion 11A of the connecting member 11. The seal portion 12A is arranged at a position where the surface thereof contacts the stopper portion 11B, thereby closing the air hole 11D of the stopper portion 11B and maintaining the airtightness inside the tube 13.

The internal valve 12B has a plurality of notches. These notches form a flow path of blood flowing in from the connecting portion 11A when the seal member 12 slides toward the tube 13 side and the internal valve 12B is pulled out from the connecting portion 11A. In the example of FIG. 4, when the seal portion 12A is viewed from the surface on the side where the internal valve 12B is provided, a cross-shaped notch is formed. The number and shape of the notches are not limited to those shown in FIG. 4, and are formed so that the flow path of blood flowing from the connecting portion 11A is secured when the internal valve 12B is pulled out from the connecting portion 11A. Just do it.

FIG. 5 is a perspective view showing a second example of the seal member 12. The seal member 12 shown in FIG. 5 is the same as that shown in FIG. 4 except for the shape of the internal valve 12B, and therefore common description is omitted.

In the internal valve 12B shown in FIG. 5, a reinforcing member 12B1 for connecting the roots of the valve divided into four parts by a cross-shaped notch is provided on the plug portion 11B side. By reinforcing the internal valve 12B with the reinforcing member 12B1, the operation of pushing the internal valve 12B into the connecting member 11 by the syringe 20 is stable. The seal member 12 including the reinforced internal valve 12B is also suitable for mass production.

<Movement of seal member>
6 and 7 are diagrams for explaining the movement of the seal member 12 when the syringe 20 is connected to the connecting member 11.

FIG. 6 is a diagram showing a state before and after the seal member 12 slides inside the connecting member 11. As shown in FIG. 6A, the seal member 12 is arranged at a position where the surface of the seal portion 12A on which the internal valve 12B is provided abuts the plug portion 11B before the syringe 20 is connected to the connecting member 11. It The internal valve 12B is in a state of being inserted inside the connecting portion 11A, and the air hole 11D of the plug portion 11B is closed.

When the sealing member 12 is pushed in by the tubular portion 22B at the tip of the syringe 20 (not shown), it slides to the end of the tubular portion 11C of the connecting member 11 as shown in FIG. 6(B). As a result, the internal valve 12B is extracted from the connecting member 11, the air hole 11D of the plug portion 11B is opened, and a flow path to the tube 13 is formed through the notch of the internal valve 12B. Further, the seal portion 12A is held by a not-shown convex portion 11C2 so as not to fall off from the cylindrical portion 11C at a position where the concave portion 11C1 is formed on the inner peripheral surface of the cylindrical portion 11C. Furthermore, in order to prevent the seal member 12 from falling off due to the discharge pressure when blood flows in, a protrusion may be provided on the convex portion 11C2 at the open end of the tubular portion 11C to hold the seal member 12.

FIG. 7 is a cross-sectional view showing a state in which the seal member 12 is pushed by the syringe 20 inside the connecting member 11. The cylindrical portion 22B in the opening 22A of the syringe 20 pushes the seal member 12 toward the tube 13 side as the inner peripheral surface of the opening 22A and the outer peripheral surface of the connecting member 11 are screwed together. Blood flowing from the syringe 20 passes through the tubular portion 22B, passes through the notch of the internal valve 12B, and flows into the tubular portion 11C. Furthermore, the blood that has flowed into the tubular portion 11C flows into the tube 13 through the gap between the recess 11C1 and the outer peripheral surface of the seal portion 12A.

FIG. 8 is a perspective view showing a state where the seal member 12 is pushed by the syringe 20 inside the connecting member 11. When the seal member 12 is pushed by the syringe 20 inside the connecting member 11, the convex portion 11C2 moves while slidingly contacting the outer peripheral surface of the seal portion 12A, and a blood path is formed between the concave portion 11C1 and the outer peripheral surface of the seal portion 12A. To form a gap. As shown in FIG. 7, the recessed portion 11C1 forms a step on the inner peripheral surface of the tubular portion 11C, but the stepped portion is removed to facilitate blood flow, and the recessed portion 11C1 is gradually increased in outer diameter. May be provided.

With the blood collection tube 10 of the present embodiment, even with a small amount of blood collected by the syringe 20, the opening of the blood collection tube 10 is closed by the plug portion 11B, and a desired amount is obtained via the connecting portion 11A and the tubular portion 11C. Blood can be injected from a syringe. For example, when a catheter for blood collection is secured, such as during surgery, a small amount of blood can be collected and regularly tested. Further, since the blood collection tube 10 of the present embodiment can inject a small amount of blood, it can be used for children or animals having a limited blood volume. Since the blood flow path from the syringe 20 to the blood collection tube 10 is formed by connecting the syringe 20 to the blood collection tube 10, the blood collected by the syringe can be easily injected into the tube 13.

10... Blood collection tube: 11... Connecting member: 11A... Connecting portion: 11B... Seal member: 12A... Seal part: 12B... Internal valve: 13... Tube: 20... Syringe: 21... Piston: 22... Syringe body

Claims (8)

A blood collection tube into which blood collected by a syringe is injected,
A tubular connecting part having one end connected to the tip of the syringe, and a plug part provided with a plurality of air holes for closing the opening of the blood collection tube at the other end of the connecting part and communicating with the outside of the blood collection tube. And a cylindrical portion that communicates with the connecting portion through the plug portion and fits into the opening of the blood collection tube, and a concave portion that reaches the end portion is formed in the circumferential direction in the inner peripheral surface opposite to the plug portion. A connecting member having a tubular portion formed with a plurality of convex portions formed in the axial direction on the inner peripheral surface of the concave portion,
A seal part that is slidable inside the tubular part and is slidably in contact with the convex part in the concave part, and is formed on the syringe side of the seal part and has a plurality of notches and is inserted into the connecting part. A seal member having an internal valve,
Blood collection tube. The seal portion slides to a position in which the tip of the syringe fits into the connecting portion from the side opposite to the internal valve and pushes the internal valve to slide-contact with the convex portion, the connecting portion, and the cut portion. A channel is formed from the syringe to the blood collection tube through a notch and an opening formed by the outer periphery of the seal portion and the recess.
The blood collection tube according to claim 1. Including anticoagulants,
The blood collection tube according to claim 1 or 2. The anticoagulant is included either in liquid, lyophilized, or dry coated form,
The blood collection tube according to claim 3. The anticoagulant includes an amount used for one or two measurements,
The blood collection tube according to claim 3 or 4. The anticoagulant is any of sodium citrate, EDTA, hirudin, BAPA, trypsin inhibitor, heparin, aprotinin,
The blood collection tube according to any one of claims 3 to 5. The volume is 3 mL or less, preferably 1.5 mL or less,
The blood collection tube according to any one of claims 1 to 6. Has a scale to measure the internal volume,
The blood collection tube according to any one of claims 1 to 7.

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