JP6608874B2 - Beauty composition - Google Patents

Description

  The present invention relates to a cosmetic composition, and more particularly to a cosmetic composition containing a pine bark extract and specific other ingredients as active ingredients.

  In recent years, research on anti-aging that prevents skin aging such as wrinkles has been actively conducted, and collagen and hyaluronic acid have attracted attention. It is known that this collagen and hyaluronic acid are produced by fibroblasts constituting the dermis of the skin.

  Examples of compositions that focus on the production of such collagen and hyaluronic acid include, for example, a collagen production promoter (see Patent Document 1) characterized by comprising β-glucan containing β1,3D glucan, and cardamom A cosmetic composition for promoting collagen production (see Patent Document 2) containing an extract of the above, and a composition for promoting hyaluronic acid production (see Patent Document 3), characterized by containing an extract extracted from a plant body of the family Chironomidae ) Has been proposed.

Japanese Patent Laying-Open No. 2015-71588 JP-A-2015-48338 JP 2013-155140 A

  An object of the present invention is to provide a cosmetic composition capable of protecting skin by reducing fibroblasts that contribute to the production of collagen and hyaluronic acid, thereby reducing skin damage due to ultraviolet rays and the like. It is in.

  As a result of intensive research and research on the ability of pine bark extract to protect fibroblasts, the present inventors can obtain a high fibroblast protective effect by combining pine bark extract and specific other components. As a result, the present invention has been completed. That is, it has been found that the fibroblast protective ability can be synergistically improved by combining a pine bark extract with a specific other component having little or no fibroblast protective ability.

That is, the present invention relates to a cosmetic composition comprising as an active ingredient a pine bark extract and at least one component selected from the group consisting of the following (a) to (e).
(A) At least one functional additive selected from aspartame, maltose, sucrose fatty acid ester, trehalose, reduced palatinose, reduced maltose, and calcium stearate (b) barley, sweet potato, long life grass, mugwort, kuzu, black ginger And at least one plant material selected from rice (c) at least one amino acid selected from alanine, arginine, asparagine, aspartic acid, cysteine, glycine, isoleucine, methionine, phenylalanine, proline, tryptophan, tyrosine, and valine (D) Vitamin A, Vitamin B1, Vitamin B2, Vitamin B6, Vitamin B12, Vitamin C, Vitamin D3, Vitamin E, Sodium Pantothenate, Niacin, Folic Acid, Biotin, Calcium, Manganese, Zinc, Copper, and C At least one cosmetic component selected at least one vitamin, minerals selected from the beam (e) hyaluronic acid, placenta, and collagen

  The cosmetic composition of the present invention has a skin protecting action and can be a skin protective agent used for skin protection.

.
The cosmetic composition of the present invention may be obtained by adding an active ingredient comprising a pine bark extract and at least one component selected from the group consisting of (a) to (e).

  The cosmetic composition of the present invention is preferably for oral use, and is preferably a tablet, capsule, powder, granule, or liquid.

  The present invention also relates to a cosmetic method (excluding medical practice) characterized by ingesting the cosmetic composition of the present invention.

  According to the cosmetic composition of the present invention, fibroblasts that contribute to the production of collagen and hyaluronic acid can be protected, and the skin can be protected from damages such as ultraviolet rays, preventing wrinkles and preventing aging (anti-aging), etc. The beauty effect can be obtained.

It is a figure which shows the result of the cell damage suppression rate by oxidative stress at the time of applying the beauty composition (pine bark extract + plant material) of this invention to normal human fibroblasts (NHDF). The upper left graph uses barley as the plant material, the upper right graph uses sweet potato as the plant material, the lower left graph uses long life grass as the plant material, The graph below shows the use of mugwort as a plant material. It is a figure which shows the result of the cell damage suppression rate by oxidative stress at the time of applying the beauty composition (pine bark extract + plant material) of this invention to normal human fibroblasts (NHDF). The upper left graph uses kuzu as the plant material, the upper right graph uses black ginger as the plant material, and the lower left graph uses rice (rice bran) as the plant material. is there. It is a figure which shows the result of the cytotoxicity suppression rate by the oxidative stress at the time of applying the beauty composition (pine bark extract + functional additive) of this invention to normal human fibroblasts (NHDF). The upper left graph uses aspartame as a functional additive, the upper right graph uses maltose as a functional additive, and the lower left graph shows sucrose fatty acid ester as a functional additive. The graph in the lower right shows that trehalose is used as a functional additive. It is a figure which shows the result of the cytotoxicity suppression rate by the oxidative stress at the time of applying the beauty composition (pine bark extract + functional additive) of this invention to normal human fibroblasts (NHDF). The upper left graph uses reduced palatinose as a functional additive, the upper right graph uses reduced maltose as a functional additive, and the lower left graph shows calcium stearate as a functional additive. Is used. It is a figure which shows the result of the cytotoxicity suppression rate by the oxidative stress at the time of applying the beauty composition (pine bark extract + amino acid) of this invention to a normal human fibroblast (NHDF). The upper left graph uses alanine as the amino acid, the upper right graph uses arginine as the amino acid, the lower left graph uses asparagine as the amino acid, and the lower right graph Aspartic acid is used as an amino acid. It is a figure which shows the result of the cytotoxicity suppression rate by the oxidative stress at the time of applying the beauty composition (pine bark extract + amino acid) of this invention to a normal human fibroblast (NHDF). The upper left graph uses cysteine as the amino acid, the upper right graph uses glycine as the amino acid, the lower left graph uses isoleucine as the amino acid, and the lower right graph , Using methionine as an amino acid. It is a figure which shows the result of the cytotoxicity suppression rate by the oxidative stress at the time of applying the beauty composition (pine bark extract + amino acid) of this invention to a normal human fibroblast (NHDF). The upper left graph uses phenylalanine as the amino acid, the upper right graph uses proline as the amino acid, the lower left graph uses tryptophan as the amino acid, and the lower right graph , Which uses tyrosine as an amino acid. It is a figure which shows the result of the cytotoxicity suppression rate by the oxidative stress at the time of applying the beauty composition (pine bark extract + amino acid) of this invention to a normal human fibroblast (NHDF), and uses valine as an amino acid It is. It is a figure which shows the result of the cell damage suppression rate by an oxidative stress at the time of applying the beauty composition (pine bark extract + vitamins and minerals) of this invention to normal human fibroblasts (NHDF). The upper left graph uses vitamin A as a vitamin, the upper right graph uses vitamin B1 as a vitamin, and the lower left graph uses vitamin B2 as a vitamin. The lower right graph uses vitamin B6 as a vitamin. It is a figure which shows the result of the cell damage suppression rate by an oxidative stress at the time of applying the beauty composition (pine bark extract + vitamins and minerals) of this invention to normal human fibroblasts (NHDF). The upper left graph uses vitamin B12 as vitamins, the upper right graph uses vitamin C as vitamins, and the lower left graph uses vitamin D3 as vitamins. The lower right graph uses vitamin E as vitamins. It is a figure which shows the result of the cell damage suppression rate by an oxidative stress at the time of applying the beauty composition (pine bark extract + vitamins and minerals) of this invention to normal human fibroblasts (NHDF). The upper left graph uses sodium pantothenate as vitamins, the upper right graph uses niacin as vitamins, the lower left graph uses folic acid as vitamins, The lower right graph uses biotin as a vitamin. It is a figure which shows the result of the cell damage suppression rate by an oxidative stress at the time of applying the beauty composition (pine bark extract + vitamins and minerals) of this invention to normal human fibroblasts (NHDF). The upper left graph uses calcium as a mineral, the upper right graph uses manganese as a mineral, the lower left graph uses zinc as a mineral, and the lower right This graph uses copper as minerals. It is a figure which shows the result of the cytotoxicity suppression rate by the oxidative stress at the time of applying the beauty composition (pine bark extract + vitamins and minerals) of this invention to normal human fibroblasts (NHDF), and it is chromium as minerals. Is used. It is a figure which shows the result of the cell damage suppression rate by an oxidative stress at the time of applying the beauty composition (pine bark extract + beauty ingredient) of this invention to a normal human fibroblast (NHDF). The upper left graph uses hyaluronic acid as a cosmetic component, the upper right graph uses placenta as a cosmetic component, and the lower left graph uses collagen as a cosmetic component.

  The cosmetic composition of the present invention contains a pine bark extract and at least one component selected from the group consisting of the following (a) to (e) (hereinafter sometimes referred to as other components) as active ingredients. It is characterized by that. The cosmetic composition of the present invention protects fibroblasts more effectively from oxidative stress by the synergistic effect of the combination of such active ingredients, suppresses the decrease in production of collagen and hyaluronic acid, and prevents skin damage due to ultraviolet rays and the like. It can reduce and effectively prevent or improve symptoms such as wrinkles and aging.

  The cosmetic composition of the present invention has a protective action for the skin (dermis) and can be a skin protective agent used for skin protection. The cosmetic composition of the present invention can also be used as a collagen production promoter or hyaluronic acid production promoter.

[Pine bark extract]
The pine bark extract as an active ingredient of the cosmetic composition of the present invention contains proanthocyanidins as one of main ingredients. Proanthocyanidins are a group of compounds composed of a condensation polymer having a degree of polymerization of 2 or more having flavan-3-ol and / or flavan-3,4-diol as a structural unit.

  Examples of the raw material for the pine bark extract used in the present invention include Pinus Martina, larch, Japanese black pine, Japanese red pine, Japanese pine, Japanese pine, Korean pine, Japanese pine, Ryukyu pine, Japanese pine, Japanese pine, Japanese white pine, etc. Of these, French coastal pine is preferred. The obtained extract may be concentrated or dried as necessary to form a liquid, a paste, or a powder. Moreover, a commercial item can be used for a pine bark extract, for example, the pine bark extract by Toyo Shinyaku Co., Ltd. can be used.

  The pine bark extract used in the present invention preferably contains a condensation polymer having a degree of polymerization of 2 or more as proanthocyanidins. In particular, proanthocyanidins containing a large amount of a condensation polymer having a low degree of polymerization are preferred. Examples of the condensation polymer having a low degree of polymerization include a condensation polymer having a degree of polymerization of 2 to 30 (2 to 30 mer), and a condensation polymer having a degree of polymerization of 2 to 10 (2 to 10 mer). Preferably, a condensation polymer having a degree of polymerization of 2 to 4 (2 to 4 mer) is more preferable. In the present specification, a polymer having a polymerization degree of 2 to 4 is referred to as OPC (oligomeric proanthocyanidin). The pine bark extract used in the present invention preferably contains 20% by mass or more of OPC, more preferably 30% by mass or more, and still more preferably 40% by mass or more.

  The pine bark extract is obtained by extracting pine bark with water or an organic solvent. When water is used, warm water or hot water is preferably used. Organic solvents used for extraction include foods or drugs such as methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, butane, acetone, hexane, cyclohexane, propylene glycol, hydrous ethanol, hydrous propylene glycol, etc. An organic solvent acceptable for the production of is preferably used. These water and organic solvents may be used alone or in combination of two or more solvents. In particular, water, ethanol, hydrous ethanol and the like are preferably used.

  The method for extracting the pine bark extract from the pine bark is not particularly limited, and for example, extraction methods such as a warm extraction method and a supercritical fluid extraction method can be used. In the method for extracting pine bark extract, a plurality of extraction methods may be combined.

[Other ingredients]
(A) Functional additive In the cosmetic composition of the present invention, together with the pine bark extract, at least selected from aspartame, maltose, sucrose fatty acid ester, trehalose, reduced palatinose, reduced maltose, and calcium stearate as an active ingredient One functional additive is preferably used. For example, aspartame, maltose, trehalose, reduced palatinose, and reduced maltose are used as sweeteners, and maltose, trehalose, reduced palatinose, and reduced maltose are also used as excipients. Sucrose fatty acid esters and calcium stearate are used as lubricants and the like.

(B) Plant material In the cosmetic composition of the present invention, together with the pine bark extract, as an active ingredient, at least one plant material selected from barley, sweet potato, long life grass, mugwort, kuzu, black ginger, and rice is used. It is preferable to use it.

  These plant materials may be any part of the plant such as leaves, stems, roots, flowers, fruits, trunks, branches, etc. In addition to the plant materials themselves (including dried products), pulverized products, juices In addition, a processed plant material such as an extract can be used. Examples of the pulverized product include powder and granules. The juice or extract may be liquid, but can also be used as a paste or dry powder. The extract can be obtained by extraction using an appropriate solvent. As the solvent, for example, water (hot water, hot water), ethanol, hydrous ethanol can be used. As these plant materials, commercially available products can be used.

  As barley, Nijo barley, Rojo barley, etc. can be used. As a site | part used as a plant raw material of this invention, a stem and a leaf are preferable and especially a young leaf is preferable.

  Sweet potato is a plant belonging to the convolvulaceae family and is generally called sweet potato. The sweet potato varieties are not particularly limited, and examples include suiou, joy white, koganesengan, white yutaka, sweet potato, and ayamurasaki. As a site | part used as a plant raw material of this invention, a stem and a leaf are preferable and especially a young leaf is preferable.

  Long-life grass is a perennial plant that is also referred to as a button-bowl, which belongs to the genus Azalea. As a site | part used as a plant raw material of this invention, a leaf and a root are preferable.

  Mugwort is a perennial plant belonging to the family Asteraceae. As a site | part used as a plant raw material of this invention, a stem and a leaf are preferable.

  Kuzu is a perennial plant of the leguminous genus Kudzu. As a site | part used as a plant raw material of this invention, a flower and a root are preferable and a flower is especially preferable. As Kuzuhana, what was collected in any process from the bud to the fully-opened flower may be used, and what was collected in each process can also be mixed and used. Although there is no restriction | limiting in particular as a kind of kuzu, Pueraria thomsonii (Pueraria thomsonii), Pueraria lobata (Pueraria lobata), Pueraria thunbergiana (Pueraria thunbergiana) etc. can be illustrated.

  Black ginger is named Kaempferia Parviflora, and is a plant belonging to the genus Banturmeric that grows naturally in Southeast Asia. As a site | part used as a plant raw material of this invention, a rhizome is preferable.

  Rice is a plant belonging to the genus Gramineae. The part used as the plant material of the present invention is preferably brown rice hulls or embryos. Specifically, it is preferable to use rice bran obtained as a by-product when white rice is refined. There is no restriction | limiting in particular as a kind of rice, A japonica seed | species, an indica seed | species, etc. can be mentioned.

(C) Amino acids In the cosmetic composition of the present invention, together with the pine bark extract, as active ingredients, alanine, arginine, asparagine, aspartic acid, cysteine, glycine, isoleucine, methionine, phenylalanine, proline, tryptophan, tyrosine, and valine It is preferable to use at least one amino acid (including a salt) selected from Examples of the salt include sodium salt and hydrochloride.

(D) Vitamins and minerals In the cosmetic composition of the present invention, together with the pine bark extract, as active ingredients, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D3, vitamin E, It is preferable to use at least one vitamin / mineral selected from sodium pantothenate, niacin, folic acid, biotin, calcium, manganese, zinc, copper, and chromium. In addition, calcium, manganese, zinc, copper, and chromium as minerals of the present invention include forms of compounds containing these metals.

(E) Cosmetic ingredient In the cosmetic composition of the present invention, it is preferable to use at least one cosmetic ingredient selected from hyaluronic acid, placenta and collagen as an active ingredient together with the pine bark extract.

  As hyaluronic acid, what was extracted from animal tissues, such as a chicken crown, and what was produced | generated by microorganisms, such as lactic acid bacteria, can be used, for example. The chicken crown has been used for food for a long time, and since there is no problem in terms of safety, a ground product of the chicken crown may be used instead of hyaluronic acid.

  As the placenta, an extract of placenta derived from mammals such as humans, pigs and horses can be used. As the extraction method, a conventionally known method can be used, and its shape such as liquid or powder is not particularly limited.

  The collagen may be animal-derived collagen or synthetic collagen. In addition to collagen protein, collagen peptide obtained by hydrolyzing collagen protein, or collagen molecule treated with protease, telopeptide moiety Atelocollagen is removed. As animal-derived collagen, collagen derived from fish is particularly preferable. Although it does not restrict | limit especially as an average molecular weight (weight average molecular weight) of collagen, For example, it is preferable that it is 500-100000, and it is more preferable that it is 1000-50000.

  The cosmetic composition of the present invention is not particularly limited as long as it contains a pine bark extract and other predetermined components and can be distinguished from other products as a product in terms of use in cosmetics. For example, the display of the fact that any of the main body, packaging, instructions, and promotional material of the product according to the present invention has a function of a beauty effect such as a skin protection effect is included in the scope of the present invention. For example, so-called health foods such as pharmaceuticals (including quasi-drugs), cosmetics, functional foods for which the indication of efficacy has been approved by prescribed organizations such as foods for specified health use, functional nutritional foods, functional labeling foods, etc. Can be mentioned. In so-called health foods, “barrier to the skin”, “protect the skin”, “for those worried about stains”, “for people worried about wrinkles”, “for skin that does not lose stress”, “ultraviolet rays” For example, a display showing “skin not to lose”, “happy with beauty”, “happy with skin”, and the like.

  The cosmetic composition of the present invention can be used for oral use or external use. The external preparation is not particularly limited as long as it is applied to the skin, scalp and the like, and the form is an ointment, cream, gel, lotion, emulsion, pack, poultice, etc. The form of skin external preparations, injections, etc. can be mentioned.

  Further, when the cosmetic composition of the present invention is used as an oral preparation, examples of the form thereof include tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, and the like. Examples include a shape agent, a round agent, a paste agent, a cream agent, a caplet agent, a gel agent, a chewable agent, and a stick agent. Among these, the forms of tablets, capsules, powders, granules, and liquids are particularly preferable. Specifically, supplements, container-packed beverages filled in PET bottles, cans, bottles, etc., instant powder beverages and instant granule beverages for dissolving and drinking in water (hot water), milk, fruit juice, green juice, etc. Can be illustrated. These are preferable in that they can be easily taken at the time of eating and the like, and can enhance palatability.

  The contents of the pine bark extract and other components (active ingredients) in the cosmetic composition of the present invention may be appropriately contained within the range where the effects are exerted.

  In general, when the cosmetic composition of the present invention is a pharmaceutical or a supplement (tablet, capsule), the active ingredient is preferably contained in an amount of 0.01 to 100% by mass in terms of dry mass, It is more preferable that 0.1-85 mass% is contained, and it is further more preferable that 0.5-70 mass% is contained.

  When the cosmetic composition of the present invention is a packaged beverage (liquid), the active ingredient is preferably contained in an amount of 0.01 to 15% by mass in terms of dry mass, and 0.03 to 12.5. More preferably, it is contained in an amount of 0.05% by mass, and more preferably 0.05-10% by mass.

  Moreover, when the cosmetic composition of the present invention is an instant powder beverage (powder) or an instant granule beverage (granule), the active ingredient is contained in an amount of 0.1 to 80% by mass in terms of dry mass. It is preferable that 0.5 to 70% by mass is included, and 1 to 60% by mass is more preferable.

  In order to exhibit the effects of the present invention more effectively, the active ingredient is preferably contained in an amount of 80% or more of the total beauty composition (excluding moisture) of the present invention in terms of dry mass, and more than 90% is contained. More preferably, it is more preferably 95% or more, and particularly preferably 100%. Furthermore, when the cosmetic composition of the present invention contains any one of the components (a) to (c), the contained component is preferably composed only of the active ingredients in the present invention. That is, when the cosmetic composition of the present invention contains, for example, the component (a) (plant material), it does not contain any plant material other than barley, sweet potato, long life grass, mugwort, kuzu, black ginger, terminaria, rice, and coconut palm. It is preferable to configure as above.

  The intake amount of the cosmetic composition of the present invention is not particularly limited, but from the viewpoint of exerting the effects of the present invention more remarkably, the intake amount of the active ingredient per day is 0.1 mg / day or more. Ingestion is preferable, intake is preferably 1 mg / day or more, more preferably 10 mg / day or more, and intake is particularly preferably 40 mg / day or more. . Although the upper limit in particular is not restrict | limited, For example, it is 4 g / day, Preferably it is 2 g / day.

  The cosmetic composition of the present invention can be stored in one container or divided into a plurality of containers, for example, as one day so that the daily intake becomes the above-mentioned intake.

  The blending mass ratio of the pine bark extract and other components is preferably in the range of 0.5: 1 to 70: 1 and in the range of 0.75: 1 to 60: 1 in terms of dry mass. Is more preferably in the range of 1: 1 to 60: 1, and particularly preferably in the range of 1: 1 to 50: 1. When the blending ratio of the pine bark extract and other components is within the above range, the effects of the present invention can be more effectively exhibited.

  The cosmetic composition of the present invention can be produced by a known formulation method by adding components other than the active ingredient acceptable as an oral preparation or an external preparation, if necessary.

  Examples of the cosmetic composition as the oral preparation of the present invention include cosmetic foods obtained by adding active ingredients to foods in addition to cosmetic foods containing active ingredients. The foodstuff which increased the active ingredient content of this invention compared with the foodstuff of this invention, and the foodstuff which added the active ingredient with respect to the foodstuff which does not contain the active ingredient of this invention normally can be mentioned. For the addition of the active ingredient, each component may be added separately, may be added simultaneously, or may be added together with other ingredients other than the active ingredient.

  Examples of the cosmetic food of the present invention include carbonated beverages, nutritional beverages, fruit beverages, lactic acid beverages, smoothies, green juice, etc .; frozen desserts such as ice cream, ice sherbet, shaved ice; soba, udon, harusame, chinese noodles Noodles such as instant noodles; rice cakes, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, bakery confectionery, etc .; Dairy products such as processed milk, fermented milk, and yogurt; salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing and other fats and processed foods; seasonings such as sauces and soy sauce; curry, stew, oyakodon, Porridge, miscellaneous cooking, Chinese rice bowl, bonito, tempura bowl, beef bowl, hayashi rice, omelet rice, oden, mabodorf Dumplings, can be mentioned Shumai, hamburger, meatballs, various sources, retort pouch foods such as various soups and the like.

  The cosmetic method of the present invention is characterized by ingesting the above-described cosmetic composition of the present invention, but does not include medical practice. Examples of the method of the present invention include a method of obtaining a beauty effect by providing the cosmetic food of the present invention in a restaurant such as a restaurant. Moreover, in an esthetic salon etc., the method of providing the cosmetic food of this invention can be mentioned as one of the treatment.

Hereinafter, the present invention will be described based on examples.
[Example 1]
1. Cell culture (1) Normal human fibroblasts (NHDF) were cultured in 10% FBS-DMEM using a 75 cm ² flask in a 37 ° C., 5% CO ₂ incubator.
(2) Cells suspended by trypsin treatment were seeded from a 75 cm ² flask to each well of 96 well plate at a cell density of 1.0 × 10 ⁴ cells / well.
(3) Pre-culture for 24 hours in a 37 ° C., 5% CO ₂ incubator.
(4) After removing the medium from each well, 200 μL of a test substance-containing medium prepared to a predetermined concentration was added and cultured in a CO ₂ incubator for 24 hours.

  In addition, about the combined sample of a pine bark extract and another component, the test substance containing culture medium of 2 times concentration was prepared, respectively, and it was set as the target density | concentration by adding 100 microliters. For the control, the test substance dissolved in DMSO was prepared using 10% FBS-DMEM containing 0.25% DMSO, and 10% FBS-DMEM was used for other test substances. Prepared.

  For the pine bark extract, a hot water extract (dry powder) of the bark of French coastal pine was used. As other components used together with the pine bark extract, substances shown in Tables 1 to 5 were used.

(5) After incubation for 24 hours, 50 μL of hydrogen peroxide prepared to 2.5 mM with serum-free DMEM was added (final concentration 500 μM). Control (+) was obtained by adding hydrogen peroxide to Control.

2. Measurement of inhibition rate of cell damage due to oxidative stress (1) 90 minutes after addition of hydrogen peroxide solution, remove medium from cultured cells, wash once with 200 μL / well of PBS, and dilute 30 times with serum-free DMEM 150 μL of the Cell Counting Kit-8 solution was added.
(2) The sample was allowed to stand in a 37 ° C., 5% CO ₂ incubator for appropriate color development, and the absorbance at 450 nm was measured.
(3) Based on the obtained data, the cytotoxicity inhibition rate (% of control) with respect to control (+) was calculated.

  % of control = (Data sample-Data blank) / (Data control-Data blank) x 100

  The results are shown in Tables 1 to 5 and FIGS. Each figure represents, from the left, the results of “control (hydrogen peroxide addition)”, “single addition of pine bark extract”, “single addition of other components”, “pine bark extract + addition of other components”. Moreover, the numerical value in description of the graph lower part shows sample concentration, for example, "pine_10" of the upper left graph of FIG. 1 shows the pine bark extract 10 (microgram / mL). The concentration unit of the additive component is μg / mL in all graphs. The vertical axis represents “% of control”.

For barley, dry pulverized powder of young leaves was used.
For sweet potatoes, dry crushed powder of young leaves was used.
For long-lived grasses, dry ground powder was used.
For mugwort, finely ground powder was used.
For kuzu, a hot water extract of flowers (dry powder) was used.
For black ginger, a 60% ethanol extract (dried powder) of rhizomes of black ginger was used.
About the rice bran, the commercial item was used.
For maqui berry, a commercially available fruit dry powder was used.

  As shown in Tables 1 to 5 and FIGS. 1 to 14, by combining the pine bark extract and specific other components of the present invention, the rate of inhibition of fibroblast damage due to oxidative stress increases synergistically. did. Therefore, according to the cosmetic composition of the present invention, fibroblasts are protected and a high cosmetic effect can be obtained.

[Example 2] (Manufacture of tablets)
A tablet (500 mg) comprising the following components was produced.
Reduced maltose 5% by mass
Collagen peptide 15% by mass
Soy protein 15% by mass
Starch 15% by mass
Pine bark extract 8% by mass
Vitamin C 1% by mass
Vitamin B1 1% by mass
Vitamin B2 1% by mass
Vitamin B6 1% by mass
1% by mass of folic acid
Zinc gluconate 1% by mass
Cellulose 2% by mass
Calcium chloride 2% by mass
Calcium stearate 1% by mass

[Example 3] (Manufacture of capsules)
The following mixture was enclosed in a soft capsule to produce a capsule.
Hyaluronic acid 150mg
Maltose 50mg
Reduced maltose 50mg
Pine bark extract 20mg
Silicon dioxide 10mg
Vitamin E 0.1mg
Niacin 0.5mg
Asparagine 0.25mg
Aspartic acid 0.25mg
Magnesium 1mg

[Example 4] (Production of granules)
The following ingredients were mixed to produce granules by a conventional method.
Fructooligosaccharide 20g
Isomaltooligosaccharide 20g
Calcium stearate 2g
1g of silicon dioxide
Pine bark extract 0.4g
Chlorogenic acid 0.1g
Niacin 0.01g
Zinc gluconate 0.001g

[Example 5] (Production of instant powder)
The following components were mixed to produce an instant powder (10 g) by a conventional method.
Trehalose 0.1g
Aspartame 0.1g
Sucrose fatty acid ester 2g
Pine bark extract 0.4g
Alanine 0.01g
Arginine 0.02g
Glycine 0.01g
0.01g isoleucine
Methionine 0.01g
Phenylalanine 0.01g
Proline 0.01g
Tryptophan 0.01g
Tyrosine 0.01g
Valine 0.02g
Glutamine 0.01g
Zinc gluconate 0.001g
0.001 g of iron chloride
Fragrance 0.001g
Calcium stearate balance

[Example 6] (Manufacture of solution)
A liquid (50 mL) comprising the following components was produced.
Porcine placenta 2000mg
Pine bark extract 400mg
Maltose 150mg
Asparagine 150mg
Aspartic acid 150mg
Isoleucine 50mg
Proline 50mg
Tryptophan 25mg
Vitamin C 0.001% by mass
Vitamin B1 0.001% by mass
Vitamin B6 0.001% by mass
Water remaining

Since the cosmetic composition of the present invention has a high cosmetic effect and can be used as an oral preparation or an external preparation, the industrial utility of the present invention is high.

Claims (3)

A cosmetic composition comprising a French coastal pine bark extract and sweet potato leaves as active ingredients.   The cosmetic composition according to claim 1, which has a skin protecting action.   The cosmetic composition according to claim 2, which is a skin protective agent used for skin protection.