JP6196073B2 - Skin beauty measurement method - Google Patents

Description

  The present invention relates to a method for measuring the beauty of skin using light, a method for estimating the amount of a skin color improving factor, and a method for screening an active ingredient for improving the skin condition.

  Realizing skin beauty is one of the most important issues in the field of beauty, and various cosmetic means such as esthetics, basic cosmetics, makeup cosmetics are used to solve this problem. Health food, cosmetic surgery, etc. exist.

Appearance of the skin is important as an index for measuring the effects of the various beauty means.
Conventionally, it has been the mainstream to evaluate the beauty of the appearance skin by a specialized panelist.

  In such a background, instead of evaluation by a specialized panelist, a method for simply and accurately evaluating the beauty of the appearance of the skin is being sought.

Among them, the following are known as evaluation methods using light.
In Patent Document 1, visible light is irradiated onto the skin from at least two directions having different incident angles, and (reflectance with respect to irradiated light having a small incident angle with respect to the skin) / (incidence with respect to the skin) at each wavelength of the spectrum of reflected light. A method is disclosed in which the reflectance of light having a large angle is calculated, and the beauty value of the skin is identified using the maximum value of the calculated values as an index.

  In Patent Document 2, (1) a process of obtaining digital image data by imaging a target skin under polarized illumination, and (2) a polarization plane orthogonal to the polarization plane of this polarized illumination under polarized illumination. A step of obtaining digital image data by imaging the same target skin by applying a polarizing filter; (3) a step of extracting specular reflection component data from the digital image data obtained in steps (1) and (2); ) A step of subjecting the specular reflection light component data extracted in step (3) to multi-resolution analysis, separating the data into a plurality of different frequency component data, and selecting a plurality of high frequency component data, (5) a plurality of selected data (6) a step of obtaining a variance of each pixel component for the reconstructed image data, (7) an average of the variance values obtained in (6) above Value and beauty of the skin Beauty method for evaluating skin, characterized in that it comprises a continuous characterizing process is disclosed.

JP 2003-161656 A Japanese Patent Laid-Open No. 2005-042929

  Since the method described in Patent Document 1 irradiates the skin with visible light from two directions and acquires respective reflected light spectra, the method described in Patent Document 2 includes polarized illumination and polarized light. Since the captured image data is acquired using a filter, it is difficult to say that both are easy to use because a special illumination device is required.

Then, this invention makes it a subject to provide the simple method of measuring the beauty of skin.
Moreover, this invention makes it a subject to provide the method of screening an effective component for the improvement of the skin state by providing the simple method of measuring the beauty of skin.

  In view of such a situation, the present inventors have intensively studied for a new index for measuring the beauty of the skin, and as a result, the intensity of light in the wavelength region of 460 to 500 nm generated from the skin. Found that it is closely related to the beauty of the skin.

Furthermore, the present inventors have found that the intensity of light in the above-mentioned wavelength region generated from the skin is related to the abundance of the skin color improving factor.
The present invention has been made based on the above findings.

That is, the present invention is a method for measuring the beauty of skin, characterized by measuring the beauty of skin using the intensity of light in the wavelength region of 460 to 500 nm generated from the skin as an index.
According to the measurement method of the present invention, it is possible to easily measure the beauty of skin by detecting the intensity of light in a specific wavelength region generated from the skin.

  In a preferred embodiment of the present invention, the skin is determined to be more beautiful as the light intensity increases.

  The measuring method of the present invention is particularly useful for measuring the beauty of skin color and skin age.

In a preferred embodiment of the present invention, the measurement method includes irradiating the skin with light having a color temperature in the range of 4500 to 5500K and measuring the intensity of the light.
The present inventors have found that the skin looks particularly beautiful as the intensity of light in the wavelength range obtained from the skin when irradiated with light in the color temperature range. Therefore, it is possible to measure the beauty of skin more accurately by using this index.

The present invention is a skin color improvement factor amount estimation method characterized by estimating a skin color improvement factor amount existing in the skin using an intensity of light in a wavelength region of 460 to 500 nm generated from the skin as an index.
According to the estimation method of the present invention, it is possible to estimate the amount of the skin color improving factor existing in the skin only by detecting the intensity of light in a specific wavelength region generated from the skin without separating the living tissue.

  In a preferred embodiment of the present invention, it is estimated that the greater the light intensity, the greater the amount of skin color improvement factor.

In a preferred embodiment of the present invention, the estimation method includes irradiating the skin with light having a color temperature in the range of 4500 to 5500K and measuring the intensity of the light.
The present inventors have found that the skin color is particularly good as the intensity of the light in the wavelength range obtained from the skin when irradiated with light in the color temperature range. Therefore, by using this index, it is possible to estimate the amount of the skin color improvement factor of the skin more accurately.
NADH is mentioned as said skin color improvement factor.

The present invention is also a method for screening an active ingredient for improving skin condition from a candidate substance, and the difference in light intensity in the wavelength region of 460 to 500 nm generated from the skin before and after application of the test substance to the living body is obtained. As an index, the active ingredient is screened.
According to this method, it is possible to evaluate the effect of the test substance on the skin by a simple method of measuring the intensity of light in a specific wavelength region generated from the skin before and after application of the test substance to the living body. Become. This makes it possible to easily screen for active ingredients.

  In a preferred embodiment of the present invention, when the intensity of the light after application of the test substance to the living body is higher than the intensity of the light before application of the test substance to the living body, the test substance is removed from the skin. Screen as an active ingredient to improve the condition.

  As an active ingredient screened by the method of the present invention, a component for improving the skin color, for example, a component having the ability to promote the production of a skin color improving factor such as NADH is preferably mentioned.

In a preferred form of the invention, the method comprises irradiating the skin with light having a color temperature in the range of 4500-5500K and measuring the intensity of the light.
By using the intensity of light in the wavelength range obtained from the skin when irradiated with light in the color temperature range, an active ingredient for improving the skin condition that contributes to the beauty of the skin in particular, It becomes possible to screen with high accuracy.

ADVANTAGE OF THE INVENTION According to this invention, the beauty of skin can be measured simply by measuring the intensity | strength of the light of the specific wavelength range obtained from skin.
In addition, according to the present invention, the amount of skin color improvement factor such as NADH can be easily estimated by measuring the intensity of light in a specific wavelength range obtained from the skin, and the result is applied to evaluation of the skin condition. can do.
Furthermore, according to the present invention, it is possible to easily screen for effective ingredients for improving the skin condition by measuring the intensity of light in a specific wavelength range obtained from the skin.
This makes it easier to evaluate the beauty and skin condition of the skin and the effects of the active ingredients of the topical skin preparation, which have been done by sensory evaluation by specialized panelists or using special devices and kits. Will be able to.

It is a figure which shows the process example of the measuring method of the beauty of the skin of this invention. It is a figure which shows an example of the measuring apparatus used for the measuring method of the beauty of the skin of this invention. It is a figure which shows the process example of the estimation method of the skin color improvement factor amount of this invention. It is a figure which shows an example of the estimation apparatus used for the estimation method of the skin color improvement factor amount of this invention. It is a face photograph at the time of irradiating a face with 13 types of light having different color temperatures. It is a figure which shows the spectrum of 27 types of light which has a color temperature of 5000K. It is a photograph at the time of irradiating a face with 27 types of light having a color temperature of 5000 K and having different spectra. It is a figure which shows the reflected light spectrum obtained from the frame | frame at the time of irradiating the said 27 types of light to a face. A face photograph with a higher skin beauty score (condition 1, condition 4) and a face photograph with a lower score (condition 9). It is a figure which shows the reflected light spectrum obtained from a test subject's forehead in each said condition. In other subjects, a facial photograph with a higher skin beauty score (conditions 1 and 5) and a facial photograph with a lower score (conditions 6 and 7). It is a figure which shows the reflected light spectrum obtained from a test subject's forehead in each said condition. In other subjects, a facial photograph with a higher skin beauty score (conditions 1 and 4) and a facial photograph with a lower score (conditions 6 and 7). It is a figure which shows the reflected light spectrum obtained from a test subject's forehead in each said condition. It is a test subject's face photograph before and after use of a skin external preparation. It is a figure which shows the reflected light spectrum obtained from a test subject's forehead before and after use of the said skin external preparation. It is a face photograph of the other test subject before and after use of a skin external preparation. It is a figure which shows the reflected light spectrum obtained from a test subject's forehead before and after use of the said skin external preparation. It is a face photograph of the other test subject before and after use of a skin external preparation. It is a figure which shows the reflected light spectrum obtained from a test subject's forehead before and after use of the said skin external preparation.

[1] Method for Measuring Skin Beauty The present invention measures skin beauty using the intensity of light in the wavelength range of 460 to 500 nm generated from the skin as an index, and measures skin beauty. Is the method.
The “beauty of skin” to be measured in the present invention is the beauty of skin that arises from a visible element. The measuring method of the present invention is particularly suitable for measuring the beauty of bare skin. Examples of the visually recognizable elements include texture, beam, smoothness, and skin color.
Among these, the present invention is useful for measuring the beauty of skin color. This is because the intensity of light in the wavelength region of 460 to 500 nm is closely related to the beauty of skin color, particularly the beauty of skin color of bare skin. As shown in the examples described later, the present inventors have newly found the relationship between the intensity of light in the wavelength region of 460 nm to 500 nm and the beauty of the skin.

Hereinafter, an embodiment of the skin beauty measurement method of the present invention will be described.
Since the beauty of the skin is relatively determined in magnitude, it is preferable to provide a reference for ensuring reproducibility in the measurement.
For example, the intensity of light in the wavelength region of 460 to 500 nm generated from the skin of a plurality of people is measured in advance, and the appearance of the skin of the plurality of people is evaluated by a professional panelist, and the result is scored. The correlation between the two is obtained.
At this time, by setting the evaluation item by the professional panel to “skin color beauty” or “skin age”, the skin color beauty and skin age can be measured.
The correlation between the intensity of light in the wavelength region of 460 to 500 nm generated from the skin and the beauty of the skin, or the beauty of the skin color and the skin age can be obtained by multivariate analysis such as regression analysis.

  In the measurement of skin beauty, by measuring the intensity of light in the wavelength region of 460 to 500 nm generated from the skin of the subject who is trying to measure the beauty of skin, and applying the measured value to the above correlation The beauty of the subject's skin can be calculated.

In addition, for the purpose of evaluating the change in the beauty of the skin due to the use of the cosmetic, the measurement value of the light intensity in the wavelength region of 460 to 500 nm generated from the subject's skin before the use of the cosmetic is used as a reference. It is preferable to measure the increase in the measured value of the light intensity in the wavelength region of 460 to 500 nm, which arises from the skin after using the cosmetics of the subject.
Thereby, it becomes possible to evaluate the effectiveness of cosmetics.

FIG. 1 shows a process example of a skin beauty measurement method using the measurement method of the present invention. That is, the intensity of light in the wavelength region of 460 to 500 nm generated from the skin of the subject whose skin beauty is to be measured is measured.
The light intensity can be measured as follows.
First, shine light on the subject's face. The light applied to the face may be light having the color temperature of light received by a person in normal life, but is preferably about 4500 to 5500K, and more preferably about 4800 to 5200K. It is particularly preferable that the light be in the vicinity of 5000K. This is because the reflected light in a specific wavelength region obtained when the light of the color temperature is applied to the face closely affects the beauty of the skin, as shown in the examples described later. In addition, a light irradiation process is implement | achieved also by putting a test subject under normal illumination.
The reflected light obtained by applying such light to the skin is spectrally resolved, and the intensity of light in the wavelength region of 460 to 500 nm is measured. The part of the face where the reflected light is measured is not particularly limited, but a relatively wide area such as a forehead or cheek can be selected.
Subsequently, the skin of the subject measured as described above is used in a formula (regression equation or the like) that represents a “relationship between the skin beauty score and the intensity of light in the wavelength range of 460 to 500 nm generated from the skin”. Substituting the intensity of the reflected light in the wavelength region of 460 to 500 nm resulting from
As a result, a skin beauty score value can be calculated.

An example of a measuring apparatus used in the measuring method of the present invention is shown in FIG.
The measuring device 1 includes a light source 2 that irradiates light on a subject's skin (indicated by a virtual line), a spectroscope 3 that splits reflected light from the subject's skin, and light that is split by the spectroscope 3. Among them, the light intensity measuring device 4 for measuring the intensity of light in the wavelength region of 460 to 500 nm, and the correlation indicating the correlation between the score of the beauty of the skin and the intensity of light in the wavelength region of 460 to 500 nm generated from the skin The storage means 5 for storing data (for example, regression equation) and the light intensity measured by the light intensity measuring device 4 are collated with the correlation data stored in the storage means 5, and the beauty of the subject's skin Score calculating means 61 in the computer 6 for calculating the score of the likelihood.
A spectrocolorimeter (CM-2600d, Konica Minolta Co., Ltd.) or the like can be used as an apparatus in which the spectroscope 3 and the light intensity measuring device 4 are integrated.

[2] Method for Estimating Skin Color Improvement Factor The present invention estimates the skin color improvement factor amount using the intensity of light in the wavelength region of 460 to 500 nm generated from the skin as an index. Is the method.
The “skin color improvement factor” to be estimated in the present invention is a factor contributing to good skin color. Examples of the skin color improving factor include NADH and elastin present in the skin. Among these, the estimation method of the present invention is suitable for estimating the NADH amount. The estimation method of the present invention is also suitable for estimating the total amount of NADH and elastin.
In addition, although it was conventionally known that NADH and elastin have autofluorescence, in order to estimate the amount of the skin color improving factor containing these components, a wavelength of 460 to 500 nm generated from the skin without isolating cells or the like A method for detecting the intensity of light in a region has never been studied.

Hereinafter, one form of the estimation method of the skin color improvement factor amount of the present invention will be described.
In advance, the intensity of light in the wavelength region of 460 to 500 nm generated from the skin of a plurality of people is measured, and the amount of skin color improvement factor is quantified by a conventionally known method, and the correlation between the two is obtained. .

For example, when the skin color improving factor is NADH, the amount of NADH contained in the skin can be quantified by the following method.
[Quantification of NADH]
Skin-derived fibroblasts are seeded on a 6-well plate at 1.0 × 10 ⁵ cells / well and cultured overnight. After culturing, lysosomal enzyme inhibitors Leupeptin and Pepstatin, which are known to have an effect of stagnation of autophagy (Reference 1: Borland B., et al, J. Biol. Chem., 285, 37415-37426, 2010) 100 μM each or DMSO (solvent control) is added, and the cells are collected after 24 hours, and the amount of NADH production in the cells is measured using a NAD / NADH assay kit (manufactured by Abcam). .

  The correlation between the light intensity in the wavelength range of 460 to 500 nm generated from the skin and the amount of the skin color improvement factor can be obtained by multivariate analysis such as regression analysis.

  In the estimation of the skin color improvement factor amount, the intensity of light in the wavelength region of 460 to 500 nm generated from the skin of the subject who is trying to estimate the skin color improvement factor amount is measured, and the measured value is applied to the above correlation. The estimated value of the subject's skin color improvement factor amount can be calculated.

In addition, for the purpose of evaluating the change in the amount of the skin color improvement factor due to the use of the cosmetic, the measurement value of the light intensity in the wavelength region of 460 to 500 nm generated from the subject's skin before the use of the cosmetic is used as a reference. The magnitude of the measured value of the light intensity in the wavelength region of 460 to 500 nm generated from the skin after using the cosmetics of the subject can be measured.
Thereby, it becomes possible to evaluate the effect of cosmetics.

FIG. 3 shows a process example of the skin color improvement factor amount estimation method using the estimation method of the present invention.
First, shine light on the subject's face. The light applied to the face can be selected under the same conditions as the beauty measurement method described above.
The reflected light obtained by applying such light to the skin is spectrally resolved, and the intensity of light in the wavelength region of 460 to 500 nm is measured.
Subsequently, an expression (regression equation or the like) indicating “the relationship between the amount of skin color improvement factor and the intensity of light in the wavelength region of 460 to 500 nm generated from the skin” created in advance is generated from the skin of the subject measured above. The intensity of reflected light in the wavelength region of 460 to 500 nm is substituted.
As a result, an estimated value of the skin color improvement factor amount can be calculated.

An example of the estimation apparatus used for the estimation method of the present invention is shown in FIG. In addition, about the structure similar to the measuring apparatus shown in FIG. 2, description is abbreviate | omitted by attaching | subjecting the same code | symbol.
The estimation device 12 includes a light source 2, a spectroscope 3, and a light intensity measurement device 4. Further, the storage means 52 stores correlation data (for example, regression equation) indicating the correlation between the amount of the skin color improvement factor and the intensity of light in the wavelength region of 460 to 500 nm generated from the skin. Further, the estimated value calculation means 62 in the computer 6 compares the light intensity measured by the light intensity measuring device 4 with the correlation data stored in the storage means 52, and the skin color improvement factor of the subject person Calculate an estimate of the quantity.

[3] Method for screening active ingredient for improvement of skin condition The present invention is a method for screening an active ingredient for improvement of skin condition from candidate substances, from the skin before and after application of a test substance to a living body. The effective ingredient is screened by using as an index the difference in the intensity of light generated in the wavelength region of 460 to 500 nm.
The method of the present invention is particularly useful for screening components that contribute to the improvement of skin color, and components that contribute to the promotion of the production of skin color improving factors such as NADH and elastin.
The advantage of the present invention is that the direct effect of the active ingredient can be easily evaluated using human skin.
For example, by applying a test substance whose safety has been confirmed to human skin for a certain period, and measuring and comparing the intensity of light in the wavelength region of 460 to 500 nm generated from the skin before and after application. It can be determined whether the test substance is effective in improving the skin color and whether it contributes to improving the skin.

  Hereinafter, the present invention will be described in more detail with reference to examples, but it is needless to say that the present invention is not limited to these examples.

<1> Analysis of relationship between color temperature and beauty of skin Thirteen kinds of light with different color temperatures between 2500 and 8000K are irradiated on the face of a specific subject (see FIG. 5), and the skin is Arranged in the order in which they look beautiful. As a result, it was found that the skin looks beautiful when exposed to light having a color temperature in the range of 4500 to 5500K, and that the skin looks most beautiful when exposed to light of 5000K.

<2> Analysis of irradiation light spectrum Subsequently, 27 types of light having a color temperature of 5000 K (see FIG. 6) with different spectra are irradiated on the face of the subject (conditions 1 to 27, see FIG. 7). Arranged in the order that the skin looks beautiful.
<3> Analysis of reflected light spectrum Subsequently, using the reflected light spectrum (FIG. 8) obtained from the forehead when the 27 types of light are applied to the face, the relationship between the beauty of the skin and the reflected light spectrum is obtained. Analyzed.
As a result, the higher the intensity of the reflected light in the wavelength region of 460 to 500 nm, the higher the evaluation of the beauty of the skin by a specialized panelist.
FIG. 9 shows a face photograph (condition 1, condition 4) taken under the higher conditions and a face photograph (condition 9) taken under the lower conditions in determining whether the skin looks beautiful. FIG. 10 shows a reflected light spectrum obtained from the subject's forehead under each of the above conditions. 9 and 10, it was found that there is a significant difference in the intensity of reflected light in the wavelength region of 460 to 500 nm between the upper condition and the lower condition in determining whether the skin looks beautiful.

Similarly, the test was conducted using two other subjects. In any case, the greater the intensity of the reflected light in the wavelength region of 460 to 500 nm obtained from the skin, the greater the evaluation of the beauty of the skin by a specialized panelist. The result was high (FIGS. 11-14).
From this, it was found that the beauty of the skin can be measured by using the intensity of reflected light in the wavelength region of 460 to 500 nm as an index.

<4> Evaluation of Active Ingredients An extract of the genus Hydrangea genus Achacha (Amacha extract) was evaluated by the following method. In addition, it has been elucidated by the present inventors that an amacha extract has a skin color improving effect and a production promoting effect on the amount of NADH.

  The subject was allowed to apply a topical skin preparation containing the following amateur extract to the face for 2 months.

<Production example of amateur extract>
100 g of a crushed product of leaves and branch tips of a cypress family Hydrangea genus Achacha was put into 4 L of 60% (volume) ethanol solvent, kept at 70 ° C. for 3 hours with gentle stirring, and then filtered. The filtrate was concentrated under reduced pressure at 40 ° C., and further dried with a vacuum drier to obtain a solid plant extract.

Before and after the use of the external preparation for skin, the face 1 was irradiated with the light of the above condition 1, and the reflected light spectrum obtained from the forehead was measured. A face photograph before and after using the external preparation for skin is shown in FIG. 15, and a reflected light spectrum is shown in FIG.
As a result, the intensity of reflected light in the wavelength region of 460 to 500 nm after use of the skin external preparation is remarkably increased, the skin color of the face is improved, and the beauty is improved as compared to before using the skin external preparation. I found out.

Similarly, the test was conducted using two other subjects. In either case, the intensity of reflected light in the wavelength region of at least 460 to 500 nm was clearly increased before and after the use of the external preparation for skin, The skin color was improved and the beauty of the skin was improved (FIGS. 17 to 20).
From this, it was found that the effectiveness of the external preparation for skin can be evaluated by using the intensity of reflected light in the wavelength region of 460 to 500 nm as an index.
In particular, it was found that by using the intensity of the reflected light in the wavelength region of 460 to 500 nm as an index, the skin color improving effect of the external preparation for skin can be easily evaluated. Moreover, it turned out that the intensity | strength of the reflected light of a wavelength range of 460-500 nm is useful in order to screen the active ingredient for skin color improvement from this result.
In addition, when combined with the knowledge that skin color is improved by increasing the amount of NADH and elastin, the intensity of reflected light in the wavelength region of 460 to 500 nm promotes the production of skin color improving factors such as NADH and elastin. It is presumed that it is useful as an index for screening active ingredients.

  The present invention can be used in scenes such as counseling and development of cosmetics in the aesthetic beauty scene.

DESCRIPTION OF SYMBOLS 1 Measuring apparatus 2 Light source 3 Spectrometer 4 Light intensity measuring device 5 Storage means 6 Score calculation means 12 Estimation apparatus 52 Storage means 62 Estimated value calculation means

Claims (10)

A method for measuring the beauty of skin, wherein the intensity of light in a wavelength region of 460 to 500 nm generated from the skin is used as an index, and the greater the intensity of the light, the more beautiful the skin is determined . The skin beauty measurement method according to claim 1, wherein the skin beauty is a skin color beauty. The skin beauty measurement method according to claim 1 or 2 , comprising irradiating the skin with light having a color temperature in a range of 4500 to 5500K and measuring the intensity of the light. Estimating the amount of skin color improvement factor, characterized by using the intensity of light in the wavelength region of 460 to 500 nm generated from the skin as an index, and estimating that the amount of skin color improvement factor present in the skin increases as the light intensity increases Method. The method for estimating a skin color improvement factor amount according to claim 4 , comprising irradiating the skin with light having a color temperature in the range of 4500 to 5500K and measuring the intensity of the light. The skin color improvement factor amount estimation method according to claim 4 or 5 , wherein the skin color improvement factor includes NADH. A method for screening active ingredients for improvement of skin condition from candidate substances,
The test substance as compared to the light intensity before application of the test substance to the living body, using as an index the difference in light intensity in the wavelength region of 460 to 500 nm generated from the skin before and after application of the test substance to the living body Screening the active substance for improving the skin condition, wherein the test substance is screened as an active ingredient for improving the skin condition when the light intensity after application to the living body is high Method. The screening method according to claim 7 , wherein the active ingredient is an ingredient for improving skin color. The screening method according to claim 7 or 8 , wherein the active ingredient is an ingredient having NADH production promoting ability. The screening method according to any one of claims 7 to 9 , comprising irradiating the skin with light having a color temperature in the range of 4500 to 5500K and measuring the intensity of the light.