JP6182294B2 - Bactericidal composition and medicine - Google Patents

Description

  The present invention relates to a bactericidal composition and a medicine containing silver particles.

  A wound surface such as an acute wound surface or a chronic wound surface is in a state in which bacteria and the like are propagated by exudate from the wound site and the like, and bacterial infection is extremely likely to occur. Therefore, generation of strong odor of methane gas system caused by inflammation, further deterioration of wounds, germs and the like occurs.

  Therefore, a composition containing an antibiotic or the like is widely used as a wound dressing used to coat the wound surface for treatment in order to suppress bacterial growth on the wound surface. However, since these antibiotics are diffused and metabolized on the wound surface at an early stage, it is difficult to maintain the bactericidal effect for a long time.

  On the other hand, silver ions are known to have an excellent bactericidal effect. Further, since silver ions and silver particles are less irritating to human skin, various low-stimulation cosmetics containing silver ions or silver particles have been developed. For example, (1) hypoallergenic cosmetics (see JP 2009-7266 A) that use silver ions and antibacterial components that are less irritating to the skin, and (2) the particle size and content of silver particles An antibacterial composition (see Japanese Patent Application Laid-Open No. 2006-169222) has been developed that exerts a continuous antibacterial effect against acne bacteria and the like by controlling.

  However, the hypoallergenic cosmetic (1) and the antibacterial composition (2) have not yet exhibited a sufficient bactericidal effect on the wound surface due to their low silver ion concentration. In such a situation, development of a highly safe bactericidal composition that has excellent bactericidal properties on the wound surface, can maintain a bactericidal effect for a long time, and is highly desired. .

JP 2009-7266 A JP 2006-169222 A

  The present invention has been made to solve such a problem, and has an excellent sterilizing effect, particularly an excellent sterilizing effect on a wound surface and the like, and further maintains the sterilizing effect for a long time. It aims at providing the bactericidal composition which can be used.

The invention made to solve the above problems is
[A] A sterilizing composition prepared by adding [B] silver particles to water,
A sterilizing composition having a silver ion concentration of 30 to 1000 ppm by mass.

  The bactericidal composition of the present invention contains [A] water and [B] silver particles. [B] When silver particles come into contact with [A] water, silver ions are released into the sterilizing composition. The sterilizing composition has a high concentration of silver ions of 30 mass ppm or more and 1000 mass ppm or less, and its concentration is higher than that of the conventional sterilizing composition. A sterilizing effect can be exhibited. Further, when silver particles and silver ions are mixed in the sterilizing composition, the silver particles remain at the wound site even after the time after application of the sterilizing composition has elapsed. Therefore, silver ions are released by moisture such as exudate and sweat from the wound site, and it is possible to exert a further excellent bactericidal effect against various germs such as the wound site, and this bactericidal effect is prolonged over a long period of time. Can be maintained. Moreover, the said bactericidal composition can suppress generation | occurrence | production of strong odor of a methane gas type | system | group produced by miscellaneous bacteria etc. by the said bactericidal effect. Furthermore, since the said bactericidal composition is also excellent in safety, it can be used with confidence for the human body. The sterilizing composition of the present invention includes [B] silver particles that are not ionized after [B] silver particles release silver ions, and [B] silver It is conceivable that all of the particles become silver ions, and no silver particles remain. [B] After the silver particles have released silver ions, the [B] silver particles that have not been ionized are partially dispersed as particles. Is preferred. This is because when silver particles and silver ions are mixed in the sterilizing composition, a further excellent sterilizing effect can be exerted against germs such as wound sites.

  [B] The average particle size (primary particle average particle size) of the silver particles is preferably 50 nm or more and 500 nm or less. [B] By making the average particle diameter of the silver particles in the above range, [B] silver ionization of the silver particles is promoted, and the silver ion concentration in the sterilizing composition can be easily adjusted to an appropriate concentration. Moreover, by setting the average particle diameter of [B] silver particles in the above range, [B] silver particles are likely to remain in the wound site and the like, and as a result, the excellent bactericidal effect of the bactericidal composition is maintained longer. can do. The primary particle average particle diameter is an average value of the diameters of the smallest unit particles that can be clearly separated from other particles constituting the silver particles, and is measured by a dynamic light scattering method.

  [B] The addition amount of silver particles is preferably 0.003% by mass or more and 10% by mass or less. [B] By making the addition amount of silver particle into the said range, the silver ion in the said bactericidal composition can be maintained at high concentration, and the outstanding bactericidal effect is realizable. Moreover, silver particle | grains remain easily in a wound site | part etc., and the bactericidal effect by the said bactericidal composition further increases. Part of the added [B] silver particles is ionized by the action of [A] water, and part of the silver particles are dispersed as they are. However, when the amount of added silver particles is small, etc., the case where all ions are ionized in the sterilizing composition is included.

  The sterilizing composition may further contain [C] a sterilizing agent. The said bactericidal composition can exhibit a bactericidal effect with respect to a wider variety of germs by containing a [C] bactericidal agent. Moreover, since the said bactericidal composition can further suppress the germ infection in a wound site | part etc. by using [B] silver particle and a bactericidal agent together, generation | occurrence | production of the odor by a germ etc. can also be suppressed effectively.

  [C] The bactericide is preferably at least one selected from the group consisting of benzalkonium chloride and acrinol. Since these bactericides have a bactericidal effect against a wide variety of bacteria, in addition to the bactericidal effect of silver particles and silver ions contained in the bactericidal composition, a further excellent bactericidal effect can be realized. Moreover, since the said bactericidal agent has little irritation | stimulation to skin and high safety | security, the said bactericidal composition can be used safely with respect to a human body.

  [C] It is good in content of a disinfectant being 0.00001 mass% or more and 0.05 mass% or less. [C] When the content of the bactericidal agent is within the above range, the bactericidal composition exhibits a more excellent bactericidal effect against a wide variety of bacteria.

  [D] It is good to further contain a hydrogel compound. [D] Since the hydrogel compound has a moisture retention function, it can maintain an appropriate moist state with respect to the wound site. As a result, skin cells are less likely to be dead due to drying of the wound surface, so that wound marks can be made inconspicuous.

  [D] The hydrogel compound may be at least one selected from the group consisting of carrageenan, alginic acid ester, alginic acid, hydroxyethyl cellulose, carboxymethyl cellulose, and polyacrylic acid. [D] By using at least one selected from the above group as the hydrogel compound, the water retention function of the bactericidal composition at the wound site and the like is further improved, so that silver ions are sustained to the wound site. Can be released. As a result, according to the bactericidal composition, the wound site can be treated in a short period of time, and the odor generated from the wound site due to bacterial propagation can be more effectively suppressed.

  The bactericidal composition of the present invention is suitably used as a medicine. Since the said bactericidal composition has a high bactericidal effect, it shows the outstanding therapeutic effect with respect to various wounds, such as a tear, a burn, and a pressure ulcer.

  The bactericidal composition of the present invention contains [B] silver particles and [A] water, and [B] silver particles come into contact with [A] water to release silver ions into the bactericidal composition. The sterilizing composition has a high concentration of silver ions of 30 mass ppm or more and 1000 mass ppm or less, and its concentration is higher than that of the conventional sterilizing composition. A sterilizing effect can be exhibited. Further, when silver particles and silver ions are mixed in the sterilizing composition, a further excellent sterilizing effect can be exerted against germs such as wound sites. Even after the time has elapsed since the application of the bactericidal composition, a small amount of silver particles remain in the wound site, so that silver ions can be released by moisture such as exudate or sweat from the wound site. Because it can. For this reason, the said bactericidal composition can maintain a bactericidal effect over a long time. Moreover, the said bactericidal composition can suppress generation | occurrence | production of strong odor of a methane gas type | system | group produced by miscellaneous bacteria etc. by the said bactericidal effect. Furthermore, since the said bactericidal composition is also excellent in safety, it can be used with confidence for the human body.

<Bactericidal composition>
The sterilizing composition contains [B] silver particles and [A] water. Furthermore, the said bactericidal composition may contain [C] bactericidal agent and / or [D] hydrogel compound as a suitable component, and may contain other arbitrary components, unless the effect of this invention is impaired. it can. When the sterilizing composition contains [B] silver particles and [A] water, the silver particles release silver ions, and particularly when the non-ionized silver particles are dispersed in the solution, Since silver ions can be effectively released from the silver particles even at the site, etc., it can exhibit an excellent bactericidal effect on the wound site and the like, and [B] sterilize by the silver particles remaining in the wound site The effect can be maintained. Furthermore, the sterilizing composition can deodorize strong methane gas-based odor caused by various germs. Hereinafter, each component will be described in detail.

<[A] water>
[A] Water is an essential component for releasing silver ions from [B] silver particles in the sterilizing composition. [A] The water is not particularly limited, and for example, pure water, tap water, mineral water, mineral spring water, well water, hot spring water, spring water, fresh water, or the like or those obtained by performing various treatments can be used. Examples of such treatment include purification, heating, sterilization, filtration, and ion exchange.

<[B] Silver particle>
In the sterilizing composition, [B] silver particles are an essential component for releasing silver ions and exhibiting a sterilizing effect. In the present invention, it is preferable that the [B] silver particles are substantially composed only of metallic silver and do not contain silver salts such as silver bromide and silver nitrate, silver composites, and the like. [B] The silver particles may be coated on the surface thereof, but are preferably not coated from the viewpoint of promoting silver ionization.

  [B] The average particle size of the silver particles is preferably 50 nm to 500 nm, more preferably 55 nm to 400 nm, and still more preferably 60 nm to 250 nm. [B] When the particle diameter of the silver particles exceeds 500 nm, the amount of silver ions released by the [B] silver particles decreases, and the silver ion concentration in the composition decreases, which may reduce the bactericidal effect. . [B] When the average particle size of the silver particles is less than 50 nm, the amount of [B] silver particles remaining on the wound site after application of the composition decreases, and as a result, the bactericidal effect of the bactericidal composition has Maintenance may be inadequate. Further, although the reason is not clear, there is a possibility that a photosensitive action such as blackening of skin or nails may occur.

  [B] The lower limit of the addition amount of silver particles is 0.003 mass%, preferably 0.01%, and more preferably 0.1 mass%. On the other hand, the upper limit of the addition amount of [B] silver particles is 10% by mass, preferably 5% by mass, and more preferably 1% by mass. [B] If the amount of silver particles added is less than the above lower limit, the number of silver particles that directly adhere to the wound or the like acts and the sterilizing effect may be insufficiently maintained. Moreover, even if the addition amount of [B] silver particle exceeds the said upper limit, since the bactericidal effect of the said bactericidal composition does not improve so much, it is unpreferable from a viewpoint of cost reduction. Furthermore, by setting the addition amount of [B] silver particles in the above range, there is an effect that [B] silver particles can be easily dispersed in the composition.

<[B] Silver particle production method>
Next, a method for producing [B] silver particles will be described. [B] The method for producing silver particles is not particularly limited, but can be produced by a general known method. Among these production methods, in order to suppress toxicity and the like due to the contamination of impurities, a production method with less contamination of impurities and a high degree of purification is preferable. In addition, commercially available silver particles may be used. However, some commercially available silver particles have a small amount of released silver ions due to surface coating treatment or the like, so care must be taken in selection. For example, the following method can be mentioned as a preferable one.

  50 g of silver placed in a shallow crucible is heated to 1000 ° C. in an electric furnace and melted. Furthermore, silver is vaporized by maintaining the temperature at 1000 ° C. At this time, by supplying an inert gas such as argon to the electric furnace and exhausting, the vaporized silver flows into the exhaust port of the electric furnace. The silver in the gas phase is captured by a filter previously attached to the exhaust port of the electric furnace, and then cooled to metal silver. Silver particles having a desired particle diameter can be obtained by such a production method. Since the silver particles produced by this production method easily generate silver ions, the cleaning composition can exhibit an excellent antibacterial effect. The reason for this is not clear, but the silver particles produced by this production method have many irregularities in the surface shape, and the surface area that can be contacted with water is larger than usual, so that silver ions are easily generated. It is thought that it has become. Also, because of its shape, it easily adheres to the skin and is not easily washed away, so it is considered that its antibacterial effect can be maintained for a long time. Furthermore, the particle diameter of the silver particles to be produced can be controlled to a size of 50 nm or more and 500 nm or less suitable for having an excellent antibacterial effect as the cleaning composition of the present invention.

<[C] fungicide>
[C] A disinfectant is a suitable component for imparting a disinfecting effect together with [B] silver particles. The bactericidal agent is not particularly limited as long as it has a bactericidal effect against various germs and the like. For example, iodine tincture, povidone iodine, sodium hypochlorite, chlorlime, mercurochrome solution, chlorhexidine gluconate, acrinol, ethanol, isopropanol, excess Examples include hydrogen oxide water (oxide), benzalkonium chloride, cetylpyridinium chloride, cresol soap solution, and the like. Among these, benzalkonium chloride or acrinol is preferable from the viewpoint of obtaining a bactericidal composition having particularly high skin safety.

  These [C] fungicides may be used alone or in combination of two or more. [C] The content of the bactericide with respect to the bactericidal composition is not particularly limited, but is preferably 0.00001% by mass or more and 0.05% by mass or less, and 0.00005% by mass or more and 0.01% by mass or less. More preferred. [C] By making content of a bactericidal agent into the said preferable range, in addition to the bactericidal effect of [B] silver particle or silver ion, the said bactericidal composition has the outstanding bactericidal effect with respect to a wider variety of germs etc. .

<Silver ion concentration>
In the sterilizing composition, the silver ion concentration is 30 mass ppm or more and 1000 mass ppm or less. When the silver ion concentration is within the above range, the bactericidal composition can exert a bactericidal effect against various bacteria at the wound site.

  The upper limit of the silver ion concentration for the sterilizing composition is 1,000 ppm by mass. As a minimum with respect to the said bactericidal composition of silver ion concentration, it is 30 mass ppm, 60 mass ppm is preferable, 100 mass ppm is more preferable, 200 mass ppm is further more preferable. Even if the silver ion concentration exceeds the above upper limit, the bactericidal effect is not improved so much from the viewpoint of cost reduction. Furthermore, if it is used at a concentration exceeding the above upper limit for the skin or the like, although there is a bactericidal effect or the like, there is a possibility that the skin or the like may turn brown, which is not preferable. Moreover, there exists a possibility that the bactericidal effect with respect to miscellaneous bacteria may fall that a silver ion concentration is less than the said minimum.

<[D] Hydrogel compound>
By adding the hydrogel compound to the bactericidal composition of the present invention, the wound site can be maintained in an appropriate moist state. Since the hydrogel compound can retain the gel structure formed by absorbing water in the composition and exudate from the wound site for a long period of time, it is effective for a long time from [B] silver particles to the wound site. Can release silver ions. As a result, the bactericidal composition can heal wound sites in a short period of time.

  The hydrogel compound is not particularly limited as long as it can form a gel structure by absorbing moisture and the like. For example, polyurethane, carboxymethylcellulose, hydroxyethylcellulose, hydroxypropylmethylcellulose, modified acrylamide, carrageenan, alginic acid ester, alginic acid, Examples include gum arabic, gelatin, starch, gum tragacanth, methylcellulose, polyacrylic acid and the like. Of these, carrageenan, alginic acid ester, alginic acid, hydroxyethylcellulose, carboxymethylcellulose, and polyacrylic acid are preferable from the viewpoint of excellent liquid absorption ability and liquid retention ability. Moreover, since the gel film by a hydrogel compound has little possibility of sticking even if it closely_contact | adheres to a wound site | part, there is almost no possibility of damaging a wound site | part when removing a gel film | membrane. As a result, the gel film formed from the sterilizing composition can be easily replaced. These compounds may be used alone or in combination of two or more.

  The content of the hydrogel compound in the sterilizing composition is not particularly limited as long as the gel structure can be maintained for a long period of time and the wound site can be maintained in an appropriate wet state, but 0.001% by mass to 5% by mass Is preferable, and 0.01 mass% or more and 1 mass% or less are more preferable. If the content of the hydrogel compound is less than the above range, the gel structure may not be maintained for a long period of time, so the time for maintaining the wound site in an appropriate moist state is shortened and the healing period is lengthened. There is a fear. Conversely, when the content of the hydrogel compound exceeds the above range, it may be difficult to apply to the wound site.

<Other optional components>
In addition to [B] silver particles, [B] water, [C] bactericidal agent and [D] hydrogel compound as suitable components, the bactericidal composition is within a range that does not impair the effects of the present invention depending on the purpose. Additives usually used as other optional components can be contained. Such additives are not particularly limited, for example, moisturizers, softeners, anti-inflammatory agents, transdermal absorption promoters, preservatives, antioxidants, thickeners, antiallergic agents, surfactants, pH A regulator or the like can be appropriately contained.

  Examples of humectants include agar, distearyl dimonium hectorite, diglycerin, butylene glycol, polyethylene glycol, propylene glycol, hexylene glycol, yocuinine extract, petrolatum, urea, hyaluronic acid, ceramide, lipid, isoflavone, amino acid, collagen , Fucodyne, lactoferrin, sorbitol, chitin / chitosan, malic acid, glucuronic acid, placenta extract, seaweed extract, button pi extract, achacha extract, hypericum extract, coleus extract, masaki extract, chorei extract, rosemary extract, hawthorn extract, Maikai flower extract, Kouca extract, Duke extract, Chamomile extract, Oyster extract, Ganoderma extract, Yarrow extract, Maronier extract, Sunaroekizu, Osmo-in extract, aloe extract, bladderwrack extract, oat extract, tuberosa polysaccharide, Cordyceps extract, barley extract, orange extract, Rehmannia glutinosa, pepper extract, Yokuininekisu, and the like.

  Examples of the softening agent include glycerin, oleic acid, glyceryl oleate, petrolatum, olive oil, squalane, lanolin, and synthetic ester oil.

  Anti-inflammatory agents include, for example, glycyrrhizic acid, glycyrrhetinic acid, phenylbutazone, indomethacin, ibuprofen, ketoprofen, allantoin, ε-aminocaproic acid, zinc oxide, diclofenac sodium, aloe extract, salvia extract, arnica extract, chamomile extract Product, birch extract, gentian root extract and the like.

  Examples of the transdermal absorption enhancer include ethanol, citric acid, adipic acid diester, diisopropyl adipate, oleic acid, octyldodecyl oleate, and isopropyl oleate.

  Examples of the preservative include benzoic acid, sorbic acid, paraben, formic acid, ethyl formate, sodium hypochlorite, phenoxyethanol, propionic acid, polylysine and the like.

  Examples of the antioxidant include ascorbic acid, α-tocopherol, γ-tocopherol, δ-tocopherol, tocotrienol, isopropyl citrate, kinetin, α-lipoic acid, polyphenol, SOD and the like.

  Examples of the thickener include hydroxypropylcellulose, hydroxypropylmethylcellulose, polyacrylic acid, polyethylene glycol, polyvinyl alcohol, polyvinylpyrrolidone, methylcellulose, ethylcellulose, pectin, gum arabic, starch and the like.

  Examples of the antiallergic agent include amlexanox, sodium cromoglycate, ozagrel hydrochloride, ibudilast, suplatast tosylate, tranilast, ketotifen fumarate and the like.

  Examples of the surfactant include an anionic surfactant, an amphoteric surfactant, a cationic surfactant, and a nonionic surfactant.

  Examples of the pH adjuster include lactic acid, citric acid, sodium citrate, sodium acetate, succinic acid, malic acid, potassium carbonate, sodium hydrogen carbonate, ammonium hydrogen carbonate and the like.

<Method for preparing bactericidal composition>
The sterilizing composition can be prepared based on a conventional method, for example, [B] silver particles and [A] water, [C] sterilizing agent and [D] hydrogel which are suitable components added as necessary. It can be obtained by mixing a compound and other optional components. The obtained sterilizing composition can be used by appropriately filling a container.

  The pH of the sterilizing composition is usually 3 or more and 9 or less, preferably 4 or more and 8 or less, and more preferably 5.5 or more and 7.5 or less. By making pH of the said bactericidal composition into the above range, there is no possibility of giving irritation to the wound site. However, since the bactericidal effect exhibited by the bactericidal composition is due to the silver ions released from the [B] silver particles, the bactericidal effect can be exhibited regardless of the pH of the bactericidal composition.

  The dosage form of the bactericidal composition is not particularly limited as long as it can contain essential components and suitable optional components and can exhibit a bactericidal effect. For example, a wide variety of dosage forms such as water-based, emulsified-based, gel-based, etc. Can be selected. For example, when the sterilizing composition is aqueous, the sterilizing composition can be put in a spray bottle container or the like and sprayed evenly over the wound site. For example, in the case where the sterilizing composition is a gel type as in the case of containing the [D] hydrogel compound, the sterilizing composition is placed in a tube container or an ointment container and applied to the wound site using a finger or a spatula. Can be used.

<Usage method of bactericidal composition>
The bactericidal composition can be used directly on the wound site, for example by means such as application or spraying to treat acute or chronic wounds. Acute wounds include, for example, incisions or cuts, lacerations, abrasions, burns, stab wounds, bruises, hematomas, crush wounds and the like caused by needles and knives. Examples of chronic wounds include venous ulcers that affect the elderly, diabetic ulcers, decubitus ulcers, and corneal ulcers resulting from bacterial infection.

  The said bactericidal composition can exhibit the outstanding bactericidal effect by the silver ion discharge | released from a [B] silver particle effectively by using it directly on a wound site | part by spraying etc. Specific examples of the target that can exert a bactericidal effect include Escherichia coli, Staphylococcus aureus, Candida, Myutans, O-157, Ringworm, Black yeast, MRSA, Multidrug-resistant Pseudomonas aeruginosa, VRE, and the like. And mycoplasma pneumoniae. In addition, the gel-based sterilizing composition can be used directly on the wound site by application, etc., forming a film by evaporating the moisture on the surface side in contact with air, and evaporating moisture on the wound site side. Since it can suppress, a wound site | part can be maintained in a moderate moist state for a long time.

  The said bactericidal composition can maintain a bactericidal effect by the [B] silver particle which remains in a wound site | part. Thereby, since the frequency | count of treatment with respect to a wound site | part reduces, the physical burden of a nurse can be reduced. Furthermore, the sterilizing composition can deodorize a strong methane gas-based odor caused by various germs. As a result, since the odor generated from patients such as decubitus ulcers that are chronic wounds is eliminated, the mental burden of the nurse can be reduced.

  The cleaning composition is not limited to these embodiments. For example, when the sterilizing composition is water-based, a synthetic resin film or the like can be wound around the wound site immediately after spraying in order to suppress moisture evaporation after spraying on the wound site. In this case, the synthetic resin film or the like can maintain the wound site in an appropriate wet state. For example, when the bactericidal composition is gel-based, it is not directly applied to the wound site, but can be applied to the wound site after being applied to a synthetic resin film or the like. .

<Pharmaceutical>
The bactericidal composition is suitably used as a medicament for treating, for example, an acute wound or a chronic wound. The sterilizing composition contains [B] silver particles and [A] water, and the [B] silver particles come into contact with [A] water, so that the sterilizing composition has 30 ppm to 1000 ppm by mass. Therefore, the drug exhibits excellent bactericidal effect against various germs such as wound sites and has a high therapeutic effect. Have. In addition, even after the time after treatment with the medicine has passed, since a trace amount of silver particles remains in the wound site, silver ions can be released by moisture such as exudate and sweat from the wound site, The therapeutic effect can be maintained for a long time. Furthermore, since the said pharmaceutical is excellent also in safety, it can be used safely with respect to the human body.

  EXAMPLES The present invention will be specifically described below with reference to examples, but the present invention is not limited to these examples.

<Preparation of bactericidal composition>
[Example 1]
50 g of silver placed in a shallow crucible was melted and vaporized by heating to 1000 ° C. in an electric furnace. An inert gas such as argon was supplied to the electric furnace for exhaustion, and the vaporized silver was captured by a filter previously attached to the exhaust port of the electric furnace, and then cooled to obtain metallic silver . By such a production method, silver particles having a particle diameter of about 80 nm could be obtained. [A] Commercially available sterilized water was used as water. 0.003 parts by mass of the above [B] silver particles were added to 100 parts by mass of [A] water, and the mixture was stirred and mixed at a low speed to obtain a sterilizing composition. The silver ion concentration in this sterilizing composition was 30 ppm. In addition, the silver ion concentration in the obtained composition was measured using the ICP emission spectroscopic analyzer.

[Examples 2 to 11 and Comparative Examples 1 to 3]
By the same procedure as in Example 1, the components shown in Table 1 were stirred and mixed in 100 parts by mass of [A] water to obtain a composition. The composition containing carrageenan was prepared by paying attention to sufficiently swell the carrageenan. The silver ion concentration of each composition is also shown in Table 1.

<Deodorizing effect>
Seven cubic boxes each having a side of about 20 cm and filled with about 10 ppm of methane gas-based odor gas were prepared. About 1.0 g of the composition S-3, S-4, S-5, s-1, s-2, or s-3 was sprayed inside them using a spray spray. Sensory evaluation was performed about the smell in the cube 3 minutes after spraying. As the evaluation criteria, “◯” indicates that no odor is observed, “Δ” indicates that the odor is slightly smelled, and “x” indicates that the odor is strongly smelled. In addition, since the sterilizing composition S-6 containing carrageenan cannot be sprayed, a sheet having a side of about 15 cm coated with about 1.0 g was put in a cube and evaluated in the same manner as described above. The evaluation results are shown in Table 2.

  As shown in Table 2, it was confirmed that the sterilizing composition has an excellent deodorizing effect. In particular, it was confirmed that it is effective to spray the water-based sterilizing composition against a bad methane gas-based odor filled in the air.

<Antimicrobial and bactericidal test>
An antibacterial and bactericidal test was performed using the bactericidal composition S-3 prepared in Example 3. This test was conducted on (1) Escherichia coli, (2) Staphylococcus aureus, (3) M. mutans, (4) Candida, (5) resistant Pseudomonas aeruginosa, (6) MRSA, and (7) ringworm did. In the test method, a solid medium was put on a sterilized petri dish at a rate of 20 mL / dish to form a flat plate, and then the previously cultured P. aeruginosa was smeared uniformly. To this, about 1.0 g of the sterilizing composition S-1 or S-3 was sprayed on a fixed medium plate by spraying. This was allowed to stand at 37 ° C., and the growth of the bacteria on the contact surface was observed after 5 minutes and 2 hours. The test results are shown in Table 3.

  It was confirmed that the sterilizing composition has an excellent sterilizing effect against all the bacteria tested. In particular, since most of the bacteria were killed after 5 minutes, it was confirmed that the bactericidal composition exhibited a bactericidal effect in a short time.

<Therapeutic effect>
Seven patients with pressure ulcers (bed sores), six patients with lacerations (cuts), two patients with abrasions, and two patients with burns (burns) were treated with the bactericidal composition. . All the results are shown in Table 4. The case where the treatment was completely cured within 5 days after the start of treatment was indicated as “◯”, the case where the recovery effect was observed, “△”, and the case where the treatment effect was not observed as “X”. Each treatment method and effect will be described below.

  Patient A had mild pressure ulcers on the skin due to Pseudomonas aeruginosa infection and methicillin-resistant Staphylococcus aureus (MRSA) infection. The bactericidal composition S-2 was soaked in gauze and applied directly to the pressure ulcer site for treatment. The gauze soaked with the bactericidal composition S-2 was changed once a day, and the progress was observed. As a result, the exudate stopped 3 days after the start of treatment, and the wound began to dry. On the seventh day, the exudate completely stopped, and the wound was recovered after drying. Two weeks later, the wound was reduced and treatment was completed.

  Patient B and Patient C had severe pressure ulcers on the skin due to Pseudomonas aeruginosa infection and methicillin resistant Staphylococcus aureus (MRSA) infection. Both types of pressure ulcers were similar. The bactericidal composition S-2 was soaked in gauze and applied directly to the pressure ulcer site for treatment. The gauze soaked with the bactericidal composition S-2 was exchanged twice daily and the progress was observed. As a result, the exudate stopped 3 days after the start of treatment, and the wound began to dry. On the seventh day, the exudate completely stopped, and the wound was recovered after drying. After 2 weeks, the above bacteria disappeared completely.

  Patients DG had mild acne due to Pseudomonas aeruginosa infection and methicillin-resistant Staphylococcus aureus (MRSA) infection on the skin. The treatment which sprays disinfectant composition S-3 directly on a pressure ulcer site | part was performed using the spray spray. For all, the Pseudomonas aeruginosa and MRSA adhesion layer at the pressure ulcer site could be removed in about 2 hours, confirming a markedly improved therapeutic effect at the pressure ulcer site.

  A treatment for directly spraying the sterilizing composition S-4 using a spray spray on the laceration site was performed on a laceration patient H, which is one of acute wounds, specifically a patient whose sole was wound with a shell. . As a result, although the degree of laceration was mild, it was completely cured about two days earlier than the case of carrying out normal general treatment, and the early treatment effect on the laceration of the bactericidal composition was confirmed.

  Two children, I and J, who were scratched, which was one of the acute wounds, were sprayed once a day with a sterilizing composition S-4 on the scratched site using a spray spray. As a result, the scratches were completely healed in 2 days.

  Two patients K and L with mild burns, one of the acute wounds, were treated by immersing the bactericidal composition S-2 in gauze and directly hitting the burn site. The gauze soaked with the bactericidal composition S-2 was changed once a day, and the progress was observed. As a result, patient K was completely cured on the third day, and patient L was completely cured on the seventh day. The early treatment effect with respect to the burn of the said bactericidal composition was able to be confirmed.

  For laceration patients M to Q, which is one of the acute wounds, a spray spray was applied to the laceration site, and the bactericidal composition shown in Table 4 was directly sprayed, and the site was covered with a film for treatment. As a result, it was completely cured about 2 days earlier than the case of carrying out normal general treatment, and the early treatment effect on the laceration of the bactericidal composition was confirmed.

  For a mild laceration patient R, the silver particles obtained in Example 1 were applied to gauze and directly applied to the laceration site for treatment (Reference Example 1). As a result, although the degree of laceration was mild, it was completely cured on the third day after treatment, and it was confirmed that the silver particles used in the present invention have a remarkable therapeutic effect on laceration.

  As described above, the bactericidal composition of the present invention showed a marked therapeutic effect on the wounds and the like. In particular, according to the method of spraying the bactericidal composition with a spray spray or the like, it was confirmed that an excellent therapeutic effect was exhibited even on wounds such as severe pressure ulcers. It should be noted that the exchange of gauze soaked with the sterilizing composition and spraying with a spray spray showed a sufficient effect once a day and did not require multiple treatments. It was thought that it had a good therapeutic effect.

As described above, the bactericidal composition of the present invention is excellent in deodorizing effect and bactericidal effect, and further has a remarkable therapeutic effect on wounds such as pressure sores, lacerations, abrasions, burns and the like. Moreover, since the said bactericidal composition is excellent also in safety, it can be used with confidence for the human body.

Claims (5)

[A] A sterilizing composition prepared by adding [B] silver particles to water,
The solid content added to the water is only silver particles,
The concentration of silver ions is 30 mass ppm or more and 1000 mass ppm or less,
The silver ions are generated by ionization of [B] silver particles added to [A] water,
[B] Silver particles are particles obtained by cooling after vaporizing silver, and the surface is not subjected to coating treatment,
[B] The primary particle average particle diameter measured by a dynamic light scattering method of silver particles is 50 nm or more and 500 nm or less,
A bactericidal composition having a pH of 5.5 to 7.5. [A] A sterilizing composition prepared by adding [B] silver particles to water,
Solid content added to water is only silver particles, [C] fungicide and / or [D] hydrogel compound,
[C] The fungicide is at least one selected from the group consisting of benzalkonium chloride and acrinol, and [D] the hydrogel compound is carrageenan, alginic acid ester, alginic acid, hydroxyethylcellulose, carboxymethylcellulose and polyacrylic acid. At least one selected from the group consisting of:
The concentration of silver ions is 30 mass ppm or more and 1000 mass ppm or less,
The silver ions are generated by ionization of [B] silver particles added to [A] water,
[B] Silver particles are particles obtained by cooling after vaporizing silver, and the surface is not subjected to coating treatment,
[B] The primary particle average particle diameter measured by a dynamic light scattering method of silver particles is 50 nm or more and 500 nm or less,
A bactericidal composition having a pH of 5.5 to 7.5.   The sterilizing composition according to claim 2, wherein the solid content added to the water contains [C] fungicide and the content of [C] fungicide is 0.00001% by mass or more and 0.05% by mass or less. [B] The sterilizing composition according to claim 1 , wherein the silver particles are added in an amount of 0.003% to 10% by mass. The pharmaceutical containing the bactericidal composition of any one of Claims 1-4 .