JP5855431B2 - Microscope equipment - Google Patents

Description

  The present invention relates to a microscope apparatus.

  2. Description of the Related Art Conventionally, there has been known a microscope apparatus in which a wavefront of light emitted from a light source is denatured by a wavefront conversion element capable of conversion, and the denatured light beam is scanned by a light beam scanning unit and incident on an objective lens (for example, Patent Documents). 1 (FIG. 2).)

JP 2001-290081 A

  In a microscope apparatus as disclosed in Patent Document 1, it is necessary to secure a sufficient optical path length from the wavefront conversion element to the light beam scanning means in order to arrange a plurality of optical components. In particular, in a microscope apparatus having a relay optical system that relays a light beam whose wavefront has been converted by a wavefront conversion element to a light beam scanning means, optical components such as a dichroic mirror for detecting light from a specimen are scanned with the relay optical system. In order to arrange them between the means, it is necessary to ensure a sufficient optical path length between the relay optical system and the light beam scanning means.

  Further, in the microscope apparatus, it is desirable that the positional relationship between the wavefront conversion element and the light beam scanning unit is conjugate to each other. However, if the optical components are arranged so that the positional relationship between the wavefront conversion element and the light beam scanning unit is conjugated with each other while sufficiently ensuring the optical path length from the relay optical system to the light beam scanning unit, the overall optical path length becomes long. As a result, there is a problem that the microscope apparatus becomes larger.

  The present invention has been made in view of the above-described circumstances, and is a microscope apparatus including a relay optical system that relays a light beam whose wavefront has been converted by a wavefront conversion element to a light beam scanning unit. The objective is to achieve downsizing while ensuring a sufficient optical path length between the dichroic mirror and arranging a dichroic mirror.

In order to achieve the above object, the present invention provides the following means.
The present invention includes a wavefront conversion element that converts a wavefront of a light beam emitted from a light source, a light beam scanning unit that scans the light beam whose wavefront has been converted by the wavefront conversion element, and a wavefront that has been converted by the wavefront conversion element. A relay optical system that relays the luminous flux to the luminous flux scanning means; an objective lens that receives the luminous flux scanned by the luminous flux scanning means; and that collects the incident luminous flux on a sample; the relay optical system; A light branching unit disposed between the light beam scanning unit and transmitting the light beam emitted from the relay optical system and reflecting the light from the sample incident through the objective lens and the light beam scanning unit; Detecting means for detecting light from the sample reflected by the branching means, the relay optical system is a first relay lens group disposed on the wavefront conversion element side; And a second relay lens group arranged in Kikotaba scanning means side, and the wavefront converting element and the light beam scanning means is arranged so as to be optically conjugate position to each other, said second relay The lens group includes two lenses, a convex lens disposed on the light beam scanning means side and a concave lens disposed on the wavefront conversion element side, and a parallel light beam is transmitted from the wavefront conversion element side to the second relay lens group. The distance from the second relay lens group to the focal position on the light beam scanning means side of the second relay lens group when incident is the parallel light flux from the light beam scanning means side to the second relay lens group. the first relay lens group rather long than the distance from the second relay lens group when is incident to the focal position of the wavefront converting element side of the second relay lens group, the wavefront converting element On the side The first relay lens when a parallel light beam is incident on the first relay lens group from the light beam scanning unit side, and the convex lens and the concave lens disposed on the light beam scanning unit side. The distance between the first relay lens group and the focal position on the wavefront conversion element side of the first relay lens group is the first relay when a parallel light beam is incident on the first relay lens group from the wavefront conversion element side. It is longer than the distance from the lens group to the focal position on the light beam scanning means side of the first relay lens group, and the focal length of the second relay lens group is shorter than the focal length of the first relay lens group. The light beam incident on the first relay lens group from the wavefront conversion element is a parallel light beam, and the distance from the wavefront conversion element to the first relay lens group is from the light beam scanning means side. The light beam scanning is shorter than the distance from the first relay lens group to the focal position on the wavefront conversion element side of the first relay lens group when a parallel light beam is incident on the first relay lens group. The distance from the second relay lens group to the second relay lens group is such that a parallel light beam is incident on the second relay lens group from the wavefront conversion element side to the second relay lens group. The distance from the wavefront conversion element to the focal position on the wavefront conversion element side of the first relay lens group is longer than the distance to the focal position on the light beam scanning means side of the group. Provided is a microscope apparatus that is longer than the distance to the focal position of the relay lens group on the light beam scanning means side .

  Here, the distance from the second relay lens group to the focal position on the light beam scanning means side of the second relay lens group when a parallel light beam is incident on the second relay lens group from the wavefront conversion element side, A relay lens group in which the distance from the second relay lens group to the focal position on the wavefront conversion element side of the second relay lens group when a parallel light beam is incident from the light beam scanning means side (hereinafter referred to as a second relay lens group) A microscope apparatus provided with a comparative example of a relay lens group is compared with the microscope apparatus according to the present invention. In this comparison, the second relay lens group of the present invention and the second relay lens group of the comparative example are compared with the second relay lens group when the parallel light flux is incident from the wavefront conversion element side. It is assumed that the distance to the focal position on the light beam scanning means side of the second relay lens group is approximately the same.

  According to the microscope apparatus of the present invention, the second relay lens group to the wavefront conversion element side of the second relay lens group when the parallel light beam is incident on the second relay lens group from the beam scanning unit side. The distance to the focal position is from the second relay lens group to the focal position on the light beam scanning means side of the second relay lens group when a parallel light beam is incident on the second relay lens group from the wavefront conversion element side. Shorter than the distance. Therefore, the optical path length of the second relay lens group of the present invention can be made shorter than that of the second relay lens group of the comparative example. Therefore, in a microscope apparatus provided with a relay optical system that relays a light beam whose wavefront has been converted by the wavefront conversion element to the light beam scanning means, a sufficient optical path length between the relay optical system and the light beam scanning means is ensured. It is possible to achieve downsizing while arranging.

  Further, in the above invention, the light beam scanning means side of the second relay lens group from the second relay lens group when a parallel light beam is incident on the second relay lens group from the wavefront conversion element side. The distance to the focal position may be longer than the focal length of the second relay lens group.

In the above invention, the second relay lens group includes a convex lens disposed on the light beam scanning unit side and a concave lens disposed on the wavefront conversion element side . In this way, when a parallel light beam is incident on the second relay lens group from the wavefront conversion element side using a convex lens arranged on the light beam scanning means side and a concave lens arranged on the wavefront conversion element side. The distance from the second relay lens group to the focal position on the light beam scanning means side of the second relay lens group is the second distance when a parallel light beam is incident on the second relay lens group from the light beam scanning means side. The distance from the relay lens group to the focal position on the wavefront conversion element side of the second relay lens group can be made longer.

  Accordingly, in a microscope apparatus including a relay optical system that relays a light beam whose wavefront has been converted by the wavefront conversion element to the light beam scanning unit, a light branching unit is provided by sufficiently securing an optical path length between the relay optical system and the light beam scanning unit. It is possible to achieve downsizing while arranging.

  Further, in the above configuration, the first position from the reference position that is equidistant from the principal point of the convex lens and the principal point of the concave lens when a parallel light beam is incident on the second relay lens group from the wavefront conversion element side. The distance between the second relay lens group and the focal position on the light beam scanning means side from the reference position when a parallel light beam is incident on the second relay lens group from the light beam scanning means side is the second relay lens. The structure may be longer than the distance to the focal position on the wavefront conversion element side of the group.

In the above invention, the wavefront conversion element side of the first relay lens group from the first relay lens group when a parallel light beam is incident on the first relay lens group from the light beam scanning means side. The light beam scanning means of the first relay lens group from the first relay lens group when the parallel light beam is incident on the first relay lens group from the wavefront conversion element side. It is longer than the distance to the focal position on the side .

  Here, the distance from the first relay lens group to the focal position on the wavefront conversion element side of the first relay lens group when the parallel light beam is incident from the light beam scanning means side, and the first from the wavefront conversion element side. The relay lens group (hereinafter referred to as the first relay lens group) in which the distance from the first relay lens group to the focal position on the light beam scanning means side of the first relay lens group when the parallel luminous flux is incident on the relay lens group A microscope apparatus provided with a comparative example of a relay lens group is compared with the microscope apparatus according to the present invention. In this comparison, the first relay lens group of the present invention and the first relay lens group of the comparative example are compared with the first relay lens group when a parallel light beam is incident from the light beam scanning means side. It is assumed that the distance to the focal position on the wavefront conversion element side of one relay lens group is approximately the same.

  According to the microscope apparatus of the present invention, the focal point on the light beam scanning means side of the first relay lens group from the first relay lens group when the parallel light beam is incident on the first relay lens group from the wavefront conversion element side. The distance to the position is the distance from the first relay lens group to the focal position on the wavefront conversion element side of the first relay lens group when the parallel light beam is incident on the first relay lens group from the light beam scanning means side. Shorter than. Therefore, the first relay lens group of the present invention can have a shorter optical path length than the first relay lens group of the comparative example. Therefore, in a microscope apparatus provided with a relay optical system that relays a light beam whose wavefront has been converted by the wavefront conversion element to the light beam scanning means, a sufficient optical path length between the relay optical system and the light beam scanning means is ensured. It is possible to achieve downsizing while arranging.

  In the above invention, the wavefront conversion element side of the first relay lens group from the first relay lens group when a parallel light beam is incident on the first relay lens group from the light beam scanning means side. The distance to the focal position may be longer than the focal length of the first relay lens group.

In the above invention, the first relay lens group includes a convex lens disposed on the wavefront conversion element side and a concave lens disposed on the light beam scanning means side . In this way, when a parallel light beam is incident on the first relay lens group from the light beam scanning unit side using the convex lens disposed on the wavefront conversion element side and the concave lens disposed on the light beam scanning unit side. The distance from the first relay lens group to the focal position on the wavefront conversion element side of the first relay lens group is the first distance when the parallel light flux is incident on the first relay lens group from the wavefront conversion element side. The distance from the relay lens group to the focal position of the first relay lens group on the light beam scanning means side can be made longer.

  Accordingly, in a microscope apparatus including a relay optical system that relays a light beam whose wavefront has been converted by the wavefront conversion element to the light beam scanning unit, a light branching unit is provided by sufficiently securing an optical path length between the relay optical system and the light beam scanning unit. It is possible to achieve downsizing while arranging.

  In the above configuration, the wavefront of the first relay lens group from a reference position that is equidistant from the principal point of the convex lens and the principal point of the concave lens when a parallel light beam is incident from the light beam scanning unit side. The distance to the focal position on the conversion element side is longer than the distance from the reference position to the focal position on the light beam scanning means side of the first relay lens group when a parallel light beam is incident from the wavefront conversion element side. It may be a configuration.

In the invention as a reference example of the present invention, the wavefront conversion element causes the wavefront conversion of the first relay lens group when a parallel light beam is incident on the first relay lens group from the light beam scanning unit side. Arranged at a focal position on the element side, the beam scanning means side of the second relay lens group when the beam scanning means makes a parallel beam incident on the second relay lens group from the wavefront conversion element side The first relay lens group and the second relay lens group are arranged at the focal position, and the first relay lens group and the second relay lens group enter the first relay lens group from the wavefront conversion element side when the parallel light flux is incident on the first relay lens group. The focal position of the relay lens group on the light beam scanning means side and the wavefront conversion element side of the second relay lens group when a parallel light beam is incident on the second relay lens group from the light beam scanning means side Focus position is It may be arranged configured for matches. By doing so, the wavefront conversion element and the light beam scanning means can be optically conjugate with each other via the first relay lens group and the second relay lens group.

Further, in the above invention, a light beam incident on the first relay lens group from the wavefront conversion element is a parallel light beam, and a distance from the wavefront conversion element to the first relay lens group is More than the distance from the first relay lens group to the focal position on the wavefront conversion element side of the first relay lens group when a parallel beam is incident on the first relay lens group from the beam scanning means side. The distance from the light beam scanning means to the second relay lens group is short from the second relay lens group when a parallel light beam is incident on the second relay lens group from the wavefront conversion element side. It is longer than the distance to the focal position of the second relay lens group on the light beam scanning means side .

  By making the excitation light beam incident on the first relay lens group from the wavefront conversion element into a parallel light beam, the distance from the wavefront conversion element to the first relay lens group is determined from the light beam scanning means side to the first relay lens. Even if it is shorter than the distance from the first relay lens group to the focal position on the wavefront conversion element side of the first relay lens group when the parallel luminous flux is incident on the group, it enters the first relay lens group. The light beam of the excitation light can be kept as a parallel light beam.

Further, the distance from the wavefront conversion element to the first relay lens group is determined from the first relay lens group when the parallel light beam is incident on the first relay lens group from the light beam scanning means side. While making the distance shorter than the distance to the focal position on the wavefront conversion element side of the group, the distance from the light beam scanning means to the second relay lens group, and the parallel light beam incident on the second relay lens group from the wavefront conversion element side By making the distance longer than the distance from the second relay lens group to the focal position on the light beam scanning means side of the second relay lens group, the wavefront conversion element and the light beam scanning means are optically conjugate with each other. The relationship that is the correct position is maintained.

  Therefore, the distance from the light beam scanning unit to the second relay lens group can be increased to ensure a sufficient optical path length between the second relay lens group and the light beam scanning unit. Therefore, it is possible to provide a microscope apparatus that achieves miniaturization while ensuring a sufficient optical path length between the relay optical system and the light beam scanning means and arranging the light branching means.

  According to the present invention, in a microscope apparatus including a relay optical system that relays a light beam whose wavefront has been converted by a wavefront conversion element to a light beam scanning unit, a sufficient optical path length is ensured between the relay optical system and the light beam scanning unit. Thus, there is an effect that downsizing can be realized while arranging the light branching means.

It is a figure which shows the microscope apparatus which concerns on 1st Embodiment as embodiment of the reference invention of this invention. It is a figure which shows the relay optical system of the microscope apparatus which concerns on 1st Embodiment as embodiment of the reference invention of this invention. It is a figure which shows the focus position of the 2nd relay lens group of the microscope apparatus which concerns on 1st Embodiment as embodiment of the reference invention of this invention. It is a figure which shows the lens structure of the Example of the relay optical system which concerns on 1st Embodiment as embodiment of the reference invention of this invention. It is a figure which shows the relay optical system of the microscope apparatus which concerns on 2nd Embodiment as embodiment of the reference invention of this invention. It is a figure which shows the lens structure of the Example of the relay optical system which concerns on 2nd Embodiment as embodiment of the reference invention of this invention. It is a figure which shows the focus position of the 1st relay lens group of the microscope apparatus which concerns on 3rd Embodiment as embodiment of the reference invention of this invention. It is a figure which shows the lens structure of the Example of the relay optical system which concerns on 3rd Embodiment as embodiment of the reference invention of this invention. It is a figure which shows the relay optical system of the microscope apparatus which concerns on 4th Embodiment as embodiment of this invention. It is a figure which shows the lens structure of the Example of the relay optical system which concerns on 4th Embodiment as embodiment of this invention.

<First Embodiment>
A microscope apparatus 1 according to a first embodiment as an embodiment of a reference invention of the present invention will be described below with reference to the drawings.
As shown in FIG. 1, the microscope apparatus 1 according to the first embodiment includes a deformable mirror (wavefront conversion element) 4 that converts a wavefront of a luminous flux of excitation light emitted from a laser light source 2, and a wavefront that is converted. A galvano mirror (light beam scanning means) 7 for scanning the emitted light beam, a relay optical system 5 for relaying the light beam whose wavefront has been converted to the galvano mirror 7, and a light beam scanned by the galvanometer mirror 7 is incident and made incident The objective lens 10 for condensing the light beam on the specimen A and the relay optical system 5 and the galvanometer mirror 7 are arranged to transmit the light beam emitted from the relay optical system 5 and through the objective lens 10 and the galvanometer mirror 7. The dichroic mirror (light branching means) 6 that reflects the fluorescence from the incident specimen A and the fluorescence from the specimen A reflected by the dichroic mirror 6 are detected. That detector and a (detecting means) 13.

  In the microscope apparatus 1, the collimator lens 3 converts the excitation light incident from the laser light source 2 into parallel light (plane wave) and emits it to the deformable mirror 4. The deformable mirror 4 converts the wavefront of the excitation light beam incident as collimated light from the collimator lens 3 and emits it to the relay optical system 5. The relay optical system 5 includes a first relay lens group 5a disposed on the deformable mirror 4 side and a second relay lens group 5b disposed on the galvano mirror 7 side.

  The light flux whose wavefront has been converted by the deformable mirror 4 is relayed to the galvanometer mirror 7 through the first relay lens group 5a and the second relay lens group 5b. Here, a dichroic mirror 6 is arranged between the second relay lens group 5b and the galvanometer mirror 7, but the dichroic mirror 6 transmits the light beam emitted from the second relay lens group 5b.

  The galvanometer mirror 7 can be swung around a swing axis (not shown), and the light beam incident from the second relay lens group 5b is scanned by the swing operation. The light beam scanned by the galvanometer mirror 7 enters the pupil position of the objective lens 10 through the pupil projection lens 8 and the imaging lens 9. The objective lens 10 collects the light beam incident on the pupil position and irradiates the specimen A. The galvanometer mirror 7 scans the light beam so that the light beam of the excitation light incident on the specimen A can be scanned.

  When the specimen A is stained with a fluorescent dye, the specimen A irradiated with the excitation light emits fluorescence, and the fluorescence enters the objective lens 10. Further, the reflected light of the excitation light itself applied to the specimen A also enters the objective lens 10. The fluorescence and the reflected light travel in the opposite direction to the excitation light along the same optical path as the optical path through which the excitation light has passed (dotted line in FIG. 1).

  Then, the fluorescence and reflected light incident on the objective lens 10 are incident on the dichroic mirror 6 via the imaging lens 9, the pupil projection lens 8, and the galvanometer mirror 7. The dichroic mirror 6 reflects fluorescence having a specific wavelength to be detected by the detector 13 and causes the reflected fluorescence to enter the condenser lens 11.

  The condensing lens 11 condenses the fluorescence incident from the dichroic mirror 6 and emits it to the detector 13. The detector 13 detects the fluorescence condensed by the condenser lens 11. Here, a pinhole 12 is disposed at a position optically conjugate with the focal position of the condenser lens 11.

  According to the microscope apparatus 1 configured as described above, the specimen A can be scanned with the excitation light emitted from the laser light source 2 and the fluorescence emitted from the specimen A can be observed.

  Next, the relay optical system 5 according to the first embodiment will be described in detail with reference to FIGS. 2 and 3. As shown in FIG. 2, the relay optical system 5 relays the excitation light beam incident as parallel light from the deformable mirror 4 to the galvanometer mirror 7 as parallel light beam. In FIG. 2, the first relay lens group 5a and the second relay lens group 5b are shown as one lens, but the first relay lens group 5a is composed of one lens. The second relay lens group 5b is composed of two lenses.

  In FIG. 2, the focal position F1 is a focal position on the galvano mirror 7 side of the second relay lens group 5b when a parallel light beam is incident from the deformable mirror 4 side, and the focal position F3 is parallel from the galvano mirror 7 side. This is the focal position on the deformable mirror 4 side of the first relay lens group 5a when the light beam is incident. The focal position F2 is a focal position on the galvano mirror 7 side of the first relay lens group 5a when a parallel light beam is incident from the deformable mirror 4 side, and a parallel light beam is incident from the galvano mirror 7 side. It is also the focal position on the deformable mirror 4 side of the second relay lens group 5b at the time.

  FIG. 3 illustrates the second relay lens group 5 b constituted by the concave lens 31 and the convex lens 30. The concave lens 31 is disposed on the deformable mirror 4 side, and the convex lens 30 is disposed on the galvano mirror 7 side. When parallel light is incident from the deformable mirror 4 side of the second relay lens group 5b, the light beam diameter is enlarged by the concave lens 31, and then the light beam diameter is reduced by the convex lens 30 and condensed at the focal position F1. This focal position F1 is a focal position on the galvano mirror 7 side of the second relay lens group 5b when a parallel light beam is incident from the deformable mirror 4 side.

  Further, when parallel light is incident from the galvanometer mirror 7 side of the second relay lens group 5b, the light beam diameter is reduced by the convex lens 30, and then the angle at which the light beam diameter is reduced by the concave lens 31 is widened to the focal position F2. Condensate. This focal position F2 is the focal position on the deformable mirror 4 side of the second relay lens group 5b when a parallel light beam is incident from the galvanometer mirror 7 side.

  Here, the principal point of the convex lens 30 is O3, the principal point of the concave lens 31 is O4, and a position where the equidistant distance d0 between O3 and O4 is a reference position C1. In this case, the distance d1 from the reference position C1 to the focal position F1 on the galvano mirror 7 side of the second relay lens group 5b is the focal position F2 on the deformable mirror 4 side of the second relay lens group 5b. It becomes longer than the distance d2.

  Note that the intersection of the extension line of parallel light incident from the deformable mirror 4 side of the second relay lens group 5b and the straight line connecting the optical path of the light emitted from the convex lens 30 is the second relay lens group 5b. Coincides with the main plane on the galvanometer mirror 7 side. The principal point 01 on the optical axis of this principal plane is the principal point on the galvano mirror 7 side of the second relay lens group 5b. The distance f1 from the principal point O1 to the focal position F1 is the focal length of the second relay lens group 5b.

  Further, the intersection of the optical path of the parallel light incident from the galvanomirror 7 side of the second relay lens group 5b and the extension line of the optical path of the light emitted from the concave lens 31 is the deformable of the second relay lens group 5b. It coincides with the main plane on the mirror 4 side. The principal point 02 on the optical axis of this principal plane is the principal point on the deformable mirror 4 side of the second relay lens group 5b. The distance from the principal point O2 to the focal position F2 is the focal length f1 of the second relay lens group 5b described above.

  As shown in FIG. 3, the distance d1 from the reference position C1 to the focal position F1 of the second relay lens group 5b is longer than the focal distance f1 of the second relay lens group 5b.

  In order to relay the luminous flux of the excitation light incident as parallel light from the deformable mirror 4 to the galvanometer mirror 7 as the parallel light flux, the deformable mirror 4 and the galvanometer mirror 7 are optically conjugate with each other. It is preferable to arrange so as to be in a position. Therefore, in the first embodiment, the deformable mirror 4 and the galvanometer mirror 7 are arranged so as to be optically conjugate with each other.

  Specifically, the deformable mirror 4 is disposed at a focal position F3 on the deformable mirror 4 side of the first relay lens group 5a, and the galvano mirror 7 is a focal position on the galvano mirror 7 side of the second relay lens group 5b. Arranged at F1. Further, the first relay lens group 5a and the second relay lens group 5b are arranged such that the focal position F2 on the galvano mirror 7 side of the first relay lens group 5a and the deformable mirror 4 side of the second relay lens group 5b. The focal positions F2 are arranged so as to coincide with each other.

  Here, a relay lens group (hereinafter, a second relay lens group 5b) in which the distance from the reference position C1 to the focal position F1 and the distance from the second relay lens group 5b to the focal position F2 are matched. The comparative example of the relay lens group is compared with the second relay lens group 5b according to the first embodiment. In this comparison, the distance from the second relay lens group 5b to the focal position F1 is the second relay lens group 5b according to the first embodiment and the second relay lens group 5b according to the comparative example. It is assumed that the distances are the same (preferably the same distance). In this case, in the first embodiment, the distance from the second relay lens group 5b to the focal position F2 is shorter than the distance from the second relay lens group 5b to the focal position F1.

  Accordingly, the optical path length of the second relay lens group 5b according to the first embodiment can be made shorter than that of the second relay lens group 5b according to the comparative example. Therefore, in the microscope apparatus 1 including the relay optical system 5 that relays the light flux whose wavefront has been converted by the deformable mirror 4 to the galvanometer mirror 7, a sufficient optical path length between the relay optical system 5 and the galvanometer mirror 7 is ensured. Thus, downsizing can be realized while arranging the dichroic mirror 6.

Next, examples of the microscope apparatus 1 according to the first embodiment as an embodiment of the reference invention of the present invention will be described below.
The lens arrangement of the microscope apparatus 1 according to the present example is shown in FIG. In FIG. 4, only part of the surface numbers are shown and others are omitted. The focal length of the first relay lens group 5a is 200 mm. The distance from the first surface S1 of the first relay lens group 5a to the focal position F3 (arrangement position of the deformable mirror 4) on the deformable mirror 4 side of the first relay lens group 5a is 199.3 mm. The distance from the second surface S2 (final surface) of the first relay lens group 5a to the focal position F2 on the galvano mirror 7 side of the first relay lens group 5a is 199.3 mm.

  The focal length of the second relay lens group 5a is 50 mm. The distance from the fourth surface S6 (final surface) of the second relay lens group 5b to the focal position F1 on the galvano mirror 4 side of the second relay lens group 5b (arrangement position of the galvano mirror 7) is 90.0 mm. The distance from the first surface S3 of the second relay lens group 5b to the focal position F2 on the deformable mirror 4 side of the second relay lens group 5b is 22.6 mm.

[Table 1]
Surface number Curvature radius Surface spacing Refractive index Abbe number deformable mirror ∞ 199.3
(First relay lens group 5a)
1 208.3 5.0 1.5225 59.5
2-208.8 221.9
(Second relay lens group 5b)
3 -10.3 4.0 1.6480 49.2
4 11.5 2.5
5 38.0 6.0 1.7552 27.6
6 -11.6

<Second Embodiment>
Next, a microscope apparatus 1 according to a second embodiment as an embodiment of a reference invention of the present invention will be described below with reference to the drawings.
In the microscope apparatus 1 according to the second embodiment, portions having the same configuration as those of the microscope apparatus 1 according to the first embodiment are denoted by the same reference numerals and description thereof is omitted. The configuration of the relay optical system 5 is the same between the microscope apparatus 1 according to the first embodiment and the microscope apparatus 1 according to the second embodiment.

  On the other hand, the deformable mirror 4 of the microscope apparatus 1 according to the second embodiment is disposed closer to the first relay lens group 5a than the deformable mirror 4 of the microscope apparatus 1 according to the first embodiment. In FIG. 5, the deformable mirror of the microscope apparatus 1 according to the first embodiment is indicated by reference numeral 4 '. Further, the galvanometer mirror 7 of the microscope apparatus 1 according to the second embodiment is arranged farther from the second relay lens group 5b than the galvanometer mirror 7 of the microscope apparatus 1 according to the first embodiment. In FIG. 5, the galvanometer mirror of the microscope apparatus 1 according to the first embodiment is denoted by reference numeral 7 '.

  In the microscope apparatus 1 according to the second embodiment, as shown in FIG. 5, the distance from the deformable mirror 4 to the first relay lens group 5a is the distance from the first relay lens group 5a to the focal position F3. The distance from the galvano mirror 7 to the second relay lens group 5b is longer than the distance from the second relay lens group 5b to the focal position F1. That is, the deformable mirror 4 is arranged at a position different from the focal position F3, and the galvano mirror 7 is arranged at a position different from the focal position F1.

  In the first embodiment, the deformable mirror 4 is disposed at the focal position F3, and the galvanometer mirror 7 is disposed at the focal position F1, so that the deformable mirror 4 and the galvanometer mirror 7 are optically conjugate with each other. It was made to become. In the microscope apparatus 1 according to the second embodiment, the distance from the deformable mirror 4 to the first relay lens group 5a is shorter than the distance from the first relay lens group 5a to the focal position F3. In this case, the position optically conjugate with the deformable mirror 4 is a position farther from the second relay lens group 5b than the focal position F1.

  In the second embodiment, the galvanometer mirror 7 is disposed at a position farther from the second relay lens group 5b than the focal position F1 and optically conjugate with the deformable mirror 4. By doing so, the relationship where the deformable mirror 4 and the galvanometer mirror 7 are optically conjugate with each other is maintained.

  Therefore, the distance from the galvanometer mirror 7 to the second relay lens group 5b can be increased, and a sufficient optical path length from the second relay lens group 5b to the galvanometer mirror 7 can be secured. Therefore, according to the second embodiment, there is provided a microscope apparatus 1 that achieves a reduction in size while ensuring a sufficient optical path length between the relay optical system 5 and the galvanometer mirror 7 and arranging the dichroic mirror 6. be able to.

Next, an example of the microscope apparatus 1 according to the second embodiment as an embodiment of the reference invention of the present invention will be described below.
The lens arrangement of the microscope apparatus 1 according to the present embodiment is shown in FIG. In FIG. 6, only part of the surface numbers are shown and others are omitted. The focal length of the first relay lens group 5a is 200 mm. The distance from the first surface S1 of the first relay lens group 5a to the position where the deformable mirror 4 is disposed is 75.0 mm, and the distance from the second surface S2 (final surface) of the first relay lens group 5a is The distance from the first relay lens group 5a to the focal position F2 on the galvano mirror 7 side is 199.3 mm.

  The focal length of the second relay lens group 5a is 50 mm. The distance from the fourth surface S6 (final surface) of the second relay lens group 5b to the arrangement position of the galvano mirror 7 is 98.4 mm, and the second surface from the first surface S3 of the second relay lens group 5b to the second. The distance from the relay lens group 5b to the focal position F2 on the deformable mirror 4 side is 22.6 mm.

[Table 2]
Surface number Curvature radius Surface spacing Refractive index Abbe number deformable mirror ∞ 75.0
(First relay lens group 5a)
1 208.3 5.0 1.5225 59.5
2-208.8 221.9
(Second relay lens group 5b)
3 -10.3 4.0 1.6480 49.2
11.5 2.5
5 38.0 6.0 1.7552 27.6
6 -11.6

<Third Embodiment>
Next, a microscope apparatus 1 according to a third embodiment as an embodiment of a reference invention of the present invention will be described below with reference to the drawings.
In the microscope apparatus 1 according to the third embodiment, portions having the same configuration as those of the microscope apparatus 1 according to the first embodiment are denoted by the same reference numerals, and description thereof is omitted. The microscope apparatus 1 according to the third embodiment and the microscope apparatus 1 according to the first embodiment are different in the configuration of the first relay lens group 5a.

  FIG. 7 illustrates the first relay lens group 5 a configured by the concave lens 41 and the convex lens 40. The concave lens 41 is disposed on the galvano mirror 7 side, and the convex lens 40 is disposed on the deformable mirror 4 side. When parallel light is incident from the galvano mirror 7 side of the first relay lens group 5a, the light beam diameter is enlarged by the concave lens 41, and then the light beam diameter is reduced by the convex lens 40 and condensed at the focal position F3. This focal position F3 is the focal position on the deformable mirror 4 side of the first relay lens group 5a when a parallel light beam is incident from the galvano mirror 7 side.

  Further, when parallel light is incident from the deformable mirror 4 side of the first relay lens group 5a, the light beam diameter is reduced by the convex lens 40, and then the angle at which the light beam diameter is reduced is widened by the concave lens 41, so that the focal position F2. Condensed to This focal position F2 becomes the focal position on the galvano mirror 7 side of the first relay lens group 5a when a parallel light beam is incident from the deformable mirror 4 side.

  Here, the principal point of the convex lens 40 is defined as O7, the principal point of the concave lens 41 is defined as O8, and a position at which O7 and O8 are equidistance d10 is defined as a reference position C10. In this case, the distance d3 from the reference position C10 to the focal position F3 on the deformable mirror 4 side of the first relay lens group 5a is the focal position F2 on the galvano mirror 7 side of the first relay lens group 5a. It becomes longer than the distance d4.

  Note that the intersection of the parallel light incident from the galvanomirror 7 side of the first relay lens group 5a and the straight line connecting the optical path of the light emitted from the convex lens 40 is the first relay lens group 5a. It coincides with the main plane on the deformable mirror 4 side. The principal point 05 on the optical axis of the principal plane is the principal point on the deformable mirror 4 side of the first relay lens group 5a. The distance f2 from the principal point O5 to the focal position F3 is the focal distance of the first relay lens group 5a.

  Further, the intersection of the optical path of the parallel light incident from the deformable mirror 4 side of the first relay lens group 5a and the extension line of the optical path of the light emitted from the concave lens 41 is the galvano of the first relay lens group 5a. It coincides with the main plane on the mirror 7 side. The principal point 06 on the optical axis of this principal plane is the principal point on the galvano mirror 7 side of the first relay lens group 5a. The distance from the principal point O6 to the focal position F2 is the focal distance f2 of the first relay lens group 5a described above.

  As shown in FIG. 7, the distance d3 from the reference position C10 to the focal position F3 of the first relay lens group 5a is larger than the focal distance f2 on the deformable mirror 4 side of the first relay lens group 5a. long.

  In order to relay the luminous flux of the excitation light incident as parallel light from the deformable mirror 4 to the galvanometer mirror 7 as the parallel light flux, the deformable mirror 4 and the galvanometer mirror 7 are optically optically coupled to each other. It is preferable to arrange them so as to be in a conjugate position. Therefore, in the third embodiment, the deformable mirror 4 and the galvanometer mirror 7 are arranged so as to be optically conjugate with each other.

  Specifically, the deformable mirror 4 is disposed at a focal position F3 on the deformable mirror 4 side of the first relay lens group 5a, and the galvano mirror 7 is a focal position on the galvano mirror 7 side of the second relay lens group 5b. Arranged at F1. Further, the first relay lens group 5a and the second relay lens group 5b are arranged such that the focal position F2 on the galvano mirror 7 side of the first relay lens group 5a and the deformable mirror 4 side of the second relay lens group 5b. The focal positions F2 are arranged so as to coincide with each other.

  Here, a relay lens group (hereinafter referred to as the first relay lens group 5a) in which the distance from the reference position C10 to the focal position F3 of the first relay lens group 5a is matched with the distance from the first relay lens group 5a to the focal position F3. The comparative example of the relay lens group is compared with the first relay lens group 5a according to the third embodiment. In this comparison, the distance from the first relay lens group 5a to the focal position F3 between the first relay lens group 5a according to the third embodiment and the first relay lens group 5a according to the comparative example is as follows. It is assumed that the distances are the same (preferably the same distance). In this case, in the third embodiment, the distance from the first relay lens group 5a to the focal position F3 is shorter than the distance from the first relay lens group 5a to the focal position F3.

  Accordingly, the optical path length of the first relay lens group 5a according to the third embodiment can be made shorter than that of the first relay lens group 5a according to the comparative example. Therefore, according to the third embodiment, in the microscope apparatus 1 including the relay optical system 5 that relays the light beam whose wavefront is converted by the deformable mirror 4 to the galvano mirror 7, the relay optical system 5 and the galvano mirror 7 The dichroic mirror 6 can be disposed while ensuring a sufficient optical path length between them, and downsizing can be realized.

Next, an example of the microscope apparatus 1 according to the third embodiment as an embodiment of the reference invention of the present invention will be described below.
The lens arrangement of the microscope apparatus 1 according to the present embodiment is shown in FIG. In FIG. 8, only part of the surface numbers are shown and others are omitted. The focal length of the first relay lens group 5a is 200 mm. Further, the distance from the first surface S1 of the first relay lens group 5a to the focal position F3 (arrangement position of the deformable mirror 4) on the deformable mirror 4 side of the first relay lens group 5a is 360.0 mm. The distance from the fourth surface S4 (final surface) of the first relay lens group 5a to the focal position F2 on the galvano mirror 7 side of the first relay lens group 5a is 90.5 mm.

  The focal length of the second relay lens group 5a is 50 mm. The distance from the fourth surface S8 (final surface) of the second relay lens group 5b to the focal position F1 on the galvano mirror 4 side of the second relay lens group 5b (arrangement position of the galvano mirror 7) is 90.0 mm. The distance from the first surface S5 of the second relay lens group 5b to the focal position F2 on the deformable mirror 4 side of the second relay lens group 5b is 22.6 mm.

[Table 3]
Surface number Curvature radius Surface spacing Refractive index Abbe number deformable mirror ∞ 360.0
(First relay lens group 5a)
1 46.2 24.0 1.7552 27.6
2-152.1 10.2
3-45.9 16.0 1.6480 49.2
4 41.3
(Second relay lens group 5b)
5 -10.3 4.0 1.6480 49.2
6 11.5 2.5
7 38.0 6.0 1.7552 27.6
8 -11.6

<Fourth Embodiment>
Next, a microscope apparatus 1 according to a fourth embodiment as an embodiment of the present invention will be described below with reference to the drawings.
The fourth embodiment is a modification of the third embodiment, and in the microscope apparatus 1 according to the fourth embodiment, the same reference numerals are used for parts having the same configuration as the microscope apparatus 1 according to the third embodiment. The description is omitted. The configuration of the relay optical system 5 is the same in the microscope apparatus 1 according to the third embodiment and the microscope apparatus 1 according to the fourth embodiment.

  On the other hand, the deformable mirror 4 of the microscope apparatus 1 according to the fourth embodiment is disposed closer to the first relay lens group 5a than the deformable mirror 4 of the microscope apparatus 1 according to the third embodiment. In FIG. 9, the deformable mirror of the microscope apparatus 1 according to the third embodiment is indicated by reference numeral 4 '. Further, the galvanometer mirror 7 of the microscope apparatus 1 according to the fourth embodiment is arranged farther from the second relay lens group 5b than the galvanometer mirror 7 of the microscope apparatus 1 according to the third embodiment. In FIG. 9, the galvanometer mirror of the microscope apparatus 1 according to the third embodiment is denoted by reference numeral 7 '.

  In the microscope apparatus 1 according to the fourth embodiment, as shown in FIG. 9, the distance from the deformable mirror 4 to the first relay lens group 5a is the distance from the first relay lens group 5a to the focal position F3. The distance from the galvano mirror 7 to the second relay lens group 5b is longer than the distance from the second relay lens group 5b to the focal position F1. That is, the deformable mirror 4 is arranged at a position different from the focal position F3, and the galvano mirror 7 is arranged at a position different from the focal position F1.

  In the third embodiment, the deformable mirror 4 is disposed at the focal position F3, and the galvanometer mirror 7 is disposed at the focal position F1, so that the deformable mirror 4 and the galvanometer mirror 7 are optically conjugate with each other. It was made to become. In the microscope apparatus 1 according to the fourth embodiment, the distance from the deformable mirror 4 to the first relay lens group 5a is shorter than the distance from the first relay lens group 5a to the focal position F3. In this case, the position optically conjugate with the deformable mirror 4 is a position farther from the second relay lens group 5b than the focal position F1.

  In the fourth embodiment, the galvanometer mirror 7 is disposed at a position farther from the second relay lens group 5b than the focal position F1 and at a position optically conjugate with the deformable mirror 4. By doing so, the relationship where the deformable mirror 4 and the galvanometer mirror 7 are optically conjugate with each other is maintained.

  Therefore, the distance from the galvanometer mirror 7 to the second relay lens group 5b can be increased, and a sufficient optical path length from the second relay lens group 5b to the galvanometer mirror 7 can be secured. Therefore, according to the fourth embodiment, there is provided a microscope apparatus 1 that achieves a reduction in size while ensuring a sufficient optical path length between the relay optical system 5 and the galvanometer mirror 7 and arranging the dichroic mirror 6. be able to.

Next, an example of the microscope apparatus 1 according to the fourth embodiment as an embodiment of the present invention will be described below.
The lens arrangement of the microscope apparatus 1 according to the present embodiment is shown in FIG. In FIG. 10, only part of the surface numbers are shown and others are omitted. The focal length of the first relay lens group 5a is 200 mm. Further, the distance from the first surface S1 of the first relay lens group 5a to the arrangement position of the deformable mirror 4 is 100.0 mm, and the distance from the fourth surface S4 (final surface) of the first relay lens group 5a is first. The distance from the first relay lens group 5a to the focal position F2 on the galvano mirror 7 side is 90.5 mm.

  The focal length of the second relay lens group 5a is 50 mm. The distance from the fourth surface S8 (final surface) of the second relay lens group 5b to the arrangement position of the galvanometer mirror 7 is 106.6 mm, and the second surface from the first surface S5 of the second relay lens group 5b to the second. The distance from the relay lens group 5b to the focal position F2 on the deformable mirror 4 side is 22.6 mm.

[Table 4]
Surface number Curvature radius Surface spacing Refractive index Abbe number deformable mirror ∞ 100.0
(First relay lens group 5a)
1 46.2 24.0 1.7552 27.6
2-152.1 10.2
3-45.9 16.0 1.6480 49.2
4 41.3
(Second relay lens group 5b)
5 -10.3 4.00 1.6480 49.2
6 11.5 2.50
7 38.0 6.00 1.7552 27.6
8 -11.6

<Other embodiments>
In the first embodiment and the second embodiment, the first relay lens group 5a is configured by one lens. However, if the optical characteristics are the same, a plurality of lenses are used. You may make it comprise. In the first embodiment, the second embodiment, the third embodiment, and the fourth embodiment, the second relay lens group 5b is composed of two lenses. If the general characteristics are the same, it may be composed of one lens or three or more lenses.

  In the first to fourth embodiments, the dichroic mirror 6 is used as the light branching unit. However, other modes may be used. For example, a half mirror that branches light according to the incident angle of light may be used as the light branching means. Further, as the light branching means, a polarization beam splitter that reflects either the S-polarized light or the P-polarized light and transmits the other may be used.

DESCRIPTION OF SYMBOLS 1 Microscope apparatus 2 Laser light source 3 Collimator lens 4 Deformable mirror 5 Relay optical system 5a 1st relay lens group 5b 2nd relay lens group 6 Dichroic mirror (light branching means)
7 Galvano mirror 8 Pupil projection lens 9 Imaging lens 10 Objective lens 11 Condensing lens 12 Pinhole 13 Detector A Sample F1 Focal position on the galvano mirror side of the second relay lens group F2 Deformable of the second relay lens group Focus position on the mirror side F3 Focus position on the deformable mirror side of the first relay lens group C1 Reference position of the second relay lens group C10 Reference position of the first relay lens group

Claims (5)

A wavefront conversion element that converts a wavefront of a light beam emitted from a light source;
A light beam scanning means for scanning the light beam whose wavefront has been converted by the wavefront conversion element;
A relay optical system that relays the light beam whose wavefront has been converted by the wavefront conversion element to the light beam scanning means;
An objective lens that receives the light beam scanned by the light beam scanning unit and collects the incident light beam on a sample;
Light that is disposed between the relay optical system and the light beam scanning means, transmits light emitted from the relay optical system, and reflects light from the sample incident through the objective lens and the light beam scanning means Branching means;
Detecting means for detecting light from the sample reflected by the light branching means,
The relay optical system includes a first relay lens group and a second relay lens group arranged in order from the wavefront conversion element side,
The wavefront conversion element and the light beam scanning means are arranged so as to be optically conjugate with each other,
The second relay lens group includes two lenses, a convex lens disposed on the light beam scanning unit side and a concave lens disposed on the wavefront conversion element side, and the second relay lens from the wavefront conversion element side. The distance from the second relay lens group to the focal position on the light beam scanning means side of the second relay lens group when a parallel light beam is incident on the group is determined from the light beam scanning means side to the second relay. the lens rather long than the distance from the second relay lens group when is incident parallel light beam to the focal position of the wavefront converting element side of the second relay lens group,
The first relay lens group includes two lenses, a convex lens disposed on the wavefront conversion element side and a concave lens disposed on the light beam scanning unit side, and the first relay lens from the light beam scanning unit side. The distance from the first relay lens group to the focal position on the wavefront conversion element side of the first relay lens group when a parallel light beam is incident on the group is the first relay from the wavefront conversion element side. Longer than the distance from the first relay lens group to the focal position on the light beam scanning means side of the first relay lens group when a parallel light beam is incident on the lens group;
The focal length of the second relay lens group is shorter than the focal length of the first relay lens group,
The light beam incident on the first relay lens group from the wavefront conversion element is a parallel light beam,
The distance from the wavefront conversion element to the first relay lens group is the first relay lens group to the first relay lens group when a parallel light beam is incident on the first relay lens group from the light beam scanning means side. Shorter than the distance to the focal position on the wavefront conversion element side of the relay lens group,
The distance from the light beam scanning means to the second relay lens group is such that the second relay lens group from the second relay lens group when the parallel light beam is incident on the second relay lens group from the wavefront conversion element side is the second distance. Longer than the distance to the focal position on the light beam scanning means side of the relay lens group,
The distance from the wavefront conversion element to the focal position on the wavefront conversion element side of the first relay lens group is larger than the distance from the light beam scanning means to the focal position on the light beam scanning means side of the second relay lens group. Long microscope device.   The distance from the second relay lens group to the focal position on the light beam scanning means side of the second relay lens group when a parallel light beam is incident on the second relay lens group from the wavefront conversion element side is The microscope apparatus according to claim 1, wherein the microscope apparatus is longer than a focal length of the second relay lens group. From the reference position that is equidistant from the principal point of the convex lens and the principal point of the concave lens when a parallel light beam is incident on the second relay lens group from the wavefront conversion element side, the second relay lens group The distance to the focal position on the light beam scanning means side is the wavefront conversion element side of the second relay lens group from the reference position when a parallel light beam is incident on the second relay lens group from the light beam scanning means side. The microscope apparatus according to claim 1 , wherein the microscope apparatus is longer than a distance to the focal position. The distance from the first relay lens group to the focal position on the wavefront conversion element side of the first relay lens group when a parallel light beam is incident on the first relay lens group from the beam scanning unit side is The microscope apparatus according to claim 1, wherein the microscope apparatus is longer than a focal length of the first relay lens group. The reference point of the first relay lens group from a reference position that is equidistant from the principal point of the convex lens and the principal point of the concave lens when a parallel light flux is incident on the first relay lens group from the light beam scanning unit side. The distance to the focal position on the wavefront conversion element side is from the reference position when the parallel light beam is incident on the first relay lens group from the wavefront conversion element side to the light beam scanning means side of the first relay lens group. The microscope apparatus according to any one of claims 1 to 4, wherein the microscope apparatus is longer than a distance to the focal position.

Images