JP5851015B2 - Analysis chip - Google Patents

Description

  The present invention relates to an analysis chip used in a photodetection method for analyzing a test substance by detecting light generated from a photoresponsive labeling substance bound to the test substance, particularly a primary reaction to a test substance such as blood. The present invention relates to an analysis chip having a function of performing processing and secondary reaction processing for detection.

  2. Description of the Related Art Conventionally, a plasmon sensor that quantitatively analyzes a substance in a sample using the principle of a surface plasmon resonance phenomenon using an evanescent wave is known (see, for example, Patent Document 1). In Patent Document 1, a light beam is irradiated at a total reflection angle on an interface between a prism and a metal film that contacts a sample provided on one surface of the prism, and the reflection angle of the light beam totally reflected at the interface is detected. It is disclosed to quantitatively analyze substances in a sample. Furthermore, in patent document 1, in order to perform the quantitative analysis of the some sample accommodated in the sample, the light source and the photon detection part are provided so that a movement is possible.

  In addition, a fluorescence detection apparatus using the above-described evanescent wave has been proposed (see, for example, Patent Document 2). Patent Document 2 discloses that a quantitative analysis of a test substance is performed by detecting fluorescence when a fluorescently labeled test substance or the like in a sample container is excited by an evanescent wave.

Japanese Patent Laid-Open No. 10-239233 JP 2009-128152 A JP 2009-222479 A

  In the case of performing biochemical analysis in a fluorescence detection apparatus as shown in Patent Document 2, generally, a primary reaction process for binding a test substance in a sample solution and a fluorescent label is performed in advance, followed by fluorescent labeling. It is necessary to perform a secondary reaction process for chemically binding the test substance.

  Here, in order to reduce the amount of the sample solution collected from the living body and to increase the detection speed, it is considered to employ μTAS (Micro Total Analysis Systems) technology as disclosed in Patent Document 3. At this time, a desired effect can be obtained for the secondary reaction treatment, but inconvenience occurs for the primary reaction treatment. In other words, the test substance in the sample solution and the fluorescent label need to be sufficiently bonded, but it is difficult to sufficiently stir and dissolve in the flow path, resulting in detection of the test substance. There is a problem that accuracy decreases.

  As described above, since preferred treatment methods are different between the primary reaction treatment and the secondary reaction treatment, it has been difficult to perform efficient measurement with the conventional method.

  Therefore, an object of the present invention is to provide an analysis chip capable of efficiently performing a primary reaction to a secondary reaction by itself.

  The analysis chip of the present invention is an analysis chip used in a photodetection method for analyzing a test substance by detecting light generated from a photoresponsive labeling substance bound to the test substance, and stores a sample solution. A pot for performing a predetermined treatment on the sample solution, a detection region for detecting light generated from the photoresponsive labeling substance, a flow path through which the sample solution flows, and an upstream side of the flow path Provided with an inlet for injecting the sample solution into the flow path, and a suction hole provided at the downstream side of the flow path for aspirating the sample solution injected from the inlet from the downstream side. It is characterized by.

  Here, the detection region includes a dielectric plate for allowing excitation light for generating an evanescent wave to enter, and a metal layer provided in a predetermined region on the specimen solution contact surface side of the dielectric plate. It is preferable that

  The pot includes a pretreatment pot for performing a predetermined pretreatment on the specimen solution, and a primary reaction treatment pot for binding the test substance and the photoresponsive labeling substance in the specimen solution. It is preferable to include.

  A predetermined dry reagent is preferably fixed to the inner surface of at least one of the pretreatment pot and the primary reaction treatment pot. At this time, it is preferable that unevenness for preventing peeling of the dry reagent is formed on the fixed portion of the dry reagent.

  Moreover, it is preferable that the opening part of the pot in which the dry reagent is adhered to the inner surface is sealed.

  Moreover, it is preferable that the inlet, the suction hole, and the pot are arranged linearly.

  Moreover, it is preferable that the detection area is composed of a plurality of detection areas arranged in a straight line.

  Further, the lower member and the upper member are formed, and a flow path is formed between the lower member and the upper member, and at least a light path portion of the light with respect to the detection region is translucent, and a pot And a cover member fitted from the upper member side of the flow path member.

  Moreover, it is preferable that the contact part of the horn for ultrasonically welding the lower member and the upper member is flat on at least one of the lower surface of the lower member and the upper surface of the upper member.

  The cover member is preferably provided with an opening in a region facing the light detection region.

  Moreover, you may display the barcode showing predetermined information on the surface of at least any one of a flow-path member and a cover member.

  Here, the “bar code” may be a one-dimensional barcode or a two-dimensional barcode.

  According to the analysis chip of the present invention, it has a pot for storing a sample solution and performing a predetermined process on the sample solution, and a detection region for detecting light generated from the photoresponsive labeling substance. A flow path through which the solution flows, an inlet provided on the upstream side of the flow path for injecting the sample solution into the flow path, and a sample solution provided on the downstream side of the flow path and injected from the inlet. Since a single analysis chip is provided with a suction hole for suctioning from the downstream side, primary reaction processing and the like can be performed in a pot, and secondary reaction processing can be performed in a flow path. It is possible to efficiently perform the primary reaction to the secondary reaction.

  Here, the detection region includes a dielectric plate for allowing excitation light for generating an evanescent wave to enter and a metal layer provided in a predetermined region on the specimen solution contact surface side of the dielectric plate. Then, it can be used for highly sensitive measurement using evanescent waves.

  In addition, a pretreatment pot for performing a predetermined pretreatment on the sample solution, and a primary reaction treatment pot for binding the test substance and the photoresponsive labeling substance in the sample solution. If included, appropriate processing can be performed using a dedicated pod for each processing.

  Here, if a predetermined dry reagent is fixed to the inner surface of at least one of the pretreatment pot and the primary reaction treatment pot, it is not necessary for the user to prepare a reagent for each treatment separately. Measurement can be performed.

  At this time, if unevenness for preventing the separation of the dry reagent is formed in the fixed portion of the dry reagent, the dry reagent can be prevented from peeling off and moving from a predetermined position when the analysis chip is moved. In particular, when the sample solution is automatically dispensed into the pod and the pretreatment or the primary reaction treatment is performed in the detection apparatus, stable treatment can be performed.

  Further, by sealing the opening of the pot where the dry reagent is fixed on the inner surface, moisture absorption or alteration of the dry reagent can be prevented.

  In addition, if the inlet, the suction hole, and the pot are arranged in a straight line, the dispensing unit is moved when the dispensing unit is moved in the detection device to automatically perform the primary reaction process to the secondary reaction process. Since it only needs to be moved in a straight line, an automatic detection device corresponding to the analysis chip of the present invention can be easily realized.

  Further, when there are a plurality of detection areas, if the plurality of detection areas are arranged in a straight line, the measurement unit is moved in a straight line when the measurement unit is moved in the detection apparatus to automatically perform the light detection process. Therefore, it is easy to realize an automatic detection device corresponding to the analysis chip of the present invention.

  In addition, a lower member and an upper member are formed, and a flow path is formed between the lower member and the upper member, and at least a light path portion of light with respect to the detection region is transparent, and a pot is formed. If the cover member is configured to be fitted from the upper member side of the flow path member, the shape of each member can be simplified and the manufacturability is also good, so that the production cost can be reduced.

  Further, if at least one of the lower surface of the lower member and the upper surface of the upper member has a flat horn contact portion for ultrasonic welding of the lower member and the upper member, It is possible to improve manufacturing reliability when ultrasonic welding is performed on the upper member.

  In addition, if an opening is provided in the area of the cover member that faces the detection area of the flow path member and light is detected from this opening, the light detection of the flow path member is performed by the thickness of the cover member near the opening. Since the portion is at a low position and the light detection portion of the flow path member is difficult to touch directly with the hand, an effect of preventing fingerprint adhesion can be obtained.

  In addition, if a bar code representing predetermined information such as individual differences in the analysis chip or the manufacturing date is displayed on the surface of at least one of the flow path member and the cover member, the analysis chip can be used using such information. Since it becomes possible to perform correction processing of errors due to individual differences of analysis chips, quality control, and the like on the measurement results obtained from the above, the merchantability of the analysis chips can be improved.

The perspective view which shows preferable embodiment of the analysis chip of this invention Top view of the analysis chip Bottom view of the analysis chip Exploded perspective view from above of the analysis chip The exploded perspective view seen from the lower part of the above-mentioned analysis chip Sectional view taken along line VI-VI in FIG. Schematic diagram showing an example of a fluorescence detection apparatus using the analysis chip Schematic configuration diagram of the measurement unit of the fluorescence detection device Block diagram of the fluorescence detection device Schematic diagram of the analysis chip FIG. 9 is a schematic diagram showing how a sample is extracted from a sample container using a nozzle tip by the sample processing means of FIG. FIG. 9 is a schematic diagram showing a state in which the sample in the nozzle tip is injected and stirred into the reagent cell by the sample processing means of FIG. Schematic diagram showing an example of the light irradiation means and the fluorescence detection means of FIG. FIG. 9 is a graph showing a state in which quantitative or qualitative analysis is performed by the rate method in the data analysis means of FIG.

  Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. 1 is a perspective view showing a preferred embodiment of the analysis chip of the present invention, FIG. 2 is a top view of the analysis chip, FIG. 3 is a bottom view of the analysis chip, and FIG. 4 is an exploded view from above the analysis chip. FIG. 5 is an exploded perspective view seen from below the analysis chip, FIG. 6 is a cross-sectional view taken along line VI-VI in FIG. 2, and FIG. 10 is a schematic diagram of the analysis chip.

  The analysis chip 10 according to the present embodiment includes a translucent lower member 11 and an upper member 12, and a flow path member in which a flow path 15 is formed between the lower member 11 and the upper member 12. The cover member 13 is fitted from the upper member 12 side of the flow path member.

  As shown in FIG. 4, recesses 11a and 11b for forming the flow path 15 are formed on the upper surface side of the lower member 11, and the flow path 15 is formed on the lower surface side of the upper member 12 as shown in FIG. In this way, when the lower member 11 and the upper member 12 are joined together, a flow path 15 is formed between them.

  The lower member 11 and the upper member 12 are made of a dielectric material such as a transparent resin, and both are joined by ultrasonic welding. However, as shown in FIGS. The area overlapping the flow path 15 on the upper surface of 12 is configured to be flat, whereby the ultrasonic welding horn can be brought into close contact, so that the reliability of ultrasonic welding can be improved. It is possible to prevent liquid leakage.

  In addition, the upper surface of the upper member 12 communicates with the inside of the flow channel 15, communicates with the inside of the flow channel 15 with an inlet 12 a for injecting the sample solution into the channel 15, and is injected from the inlet 12 a. A suction hole 12b for sucking the sample solution from the downstream side is formed.

  On the upper surface side of the cover member 13, a pretreatment pot 13 a for performing a predetermined pretreatment on the specimen solution, and a primary for binding the test substance and the photoresponsive labeling substance in the specimen solution. A reaction processing pot 13b, an inlet insertion hole 13c for inserting the inlet 12a, and a suction hole insertion hole 13d for inserting the suction hole 12b are formed.

  The pretreatment pot 13a is a container for storing a dry reagent for performing a pretreatment such as adjusting the pH to be suitable for downstream reaction with respect to the specimen, and the primary reaction treatment pot 13b is bound to the specimen. This is a container for storing a fluorescent (second antibody) dry reagent. These pots are independent containers that do not communicate with the flow path 15.

  As shown in FIG. 12, the fixed portion of the dry reagent DC is formed with uneven portions 13g for preventing the dry reagent DC from being peeled off. As a result, the dry reagent DS is peeled off when the analysis chip 10 is moved. Since movement from a predetermined position can be prevented, stable processing can be performed, particularly when the sample solution is automatically dispensed into the pod and subjected to pretreatment or primary reaction treatment.

  The openings of the pretreatment pot 13a and the primary reaction treatment pot 13b are sealed with a seal member S, and the seal member is pierced when a predetermined process is performed on the specimen. Yes. As a result, moisture absorption or alteration of the dry reagent can be prevented.

  The pretreatment pot 13a, the primary reaction treatment pot 13b, the inlet insertion hole 13c, and the suction hole insertion hole 13d are arranged in a straight line adjacent to each other. When moving from the primary reaction process to the secondary reaction process automatically, it is only necessary to move the dispensing unit in a short distance in a straight line. Therefore, the automatic detection device corresponding to the analysis chip of the present invention Realization is easy. Moreover, since the pretreatment pot 13a, the primary reaction treatment pot 13b, and the inlet 12a (inlet insertion hole 13c) into the flow path 15 for the secondary reaction treatment are arranged in this order, the stages of each treatment are It is only necessary to move the dispensing unit in one direction each time it travels, and a very efficient measurement can be performed.

  In the present embodiment, the two pots, that is, the pretreatment pot 13a and the primary reaction treatment pot 13b are provided. However, the present invention is not necessarily limited to such an embodiment, and the pretreatment pot 13a is not necessarily limited thereto. However, the pre-processing may be performed at a place different from the analysis chip 10.

  As schematically shown in FIG. 10, the injection port 12a communicates with the suction hole 12b through the flow channel 15, and the sample is injected from the injection port 12a by applying a negative pressure from the suction hole 12b. 15 is discharged from the suction hole 12b.

  Further, a test region TR for detecting a test substance in the specimen and a control region CR provided on the downstream side of the test region TR are formed in the flow path 15. A first antibody is immobilized on the test region TR, and the labeled antibody is captured by a so-called sandwich method. In addition, a reference antibody is fixed to the control region CR, and the reference antibody captures the fluorescent substance when the sample solution flows on the control region CR. Two control regions CR are formed, a so-called negative control region CR for detecting non-specific adsorption, and a so-called positive control region CR for detecting a difference in reactivity due to a difference in specimen. Is formed. These test region TR and two control regions CR function as a secondary reaction region.

  Note that the test area TR and the two control areas CR are arranged in a straight line, so that when the measurement unit is moved in the detection apparatus to automatically perform the light detection process, the measurement unit is moved in a straight line. Therefore, it is easy to realize an automatic detection device corresponding to the analysis chip of the present invention.

  The flow path 15 includes a linear detection region portion including the test region TR and two control regions CR, an introduction portion connecting the upstream end of the detection region portion to the injection port 12a, and a downstream end of the detection region portion. It has a substantially U-shape consisting of a discharge part connecting up to the suction hole 12b, and a pretreatment pot 13a and a primary reaction treatment pot 13b are arranged between the inlet 12a and the suction hole 12b. Yes. The arrangement direction of the pretreatment pot 13a, the primary reaction treatment pot 13b, the inlet insertion hole 13c, and the suction hole insertion hole 13d and the linear detection region portion are parallel to each other. By adopting such an arrangement mode, the size of the analysis chip 10 can be minimized.

  As shown in FIG. 1, four openings 13 e are provided in a region overlapping the detection region of the cover member 13. Of these, the three openings 13e correspond to the test region TR and the two control regions CR, and further one opening 13e is provided on the downstream side thereof.

  The most downstream opening 13e is used to see whether the tip of the sample solution has reached by light transmission of the LED. For example, when light is emitted by the LED from below to above the analysis chip 10 and the light amount of the LED is detected at the top, the sample solution does not reach the position of the most downstream opening 13e. A part (about 4%) of the LED light is reflected at the boundary between the lower member 11 and the flow channel and the boundary between the flow channel and the upper member 12 to finally reduce the light amount by about 8%. However, when the sample solution reaches the most downstream opening 13e, the refractive index of the sample solution and the transparent resin are close to each other, and the LED light is not reflected, so that the amount of light hardly decreases. Therefore, when the sample solution reaches the most downstream opening 13e, the detected light amount of the LED is about 8% higher than when the sample solution does not reach. In this way, it is possible to know whether the tip of the sample solution has reached the most downstream opening 13e (that is, whether all of the detection region has passed) by the change in the amount of light detected by the LED.

  Further, when the opening 13e corresponding to the test region TR, the two control regions CR, and the LED light detection unit is provided in this way, the light detection unit of the flow path member is equal to the thickness of the cover member 13 near the opening 13e. Is low, and the light detection part of the flow path member is difficult to touch directly with the hand, so that the effect of preventing fingerprint adhesion to the flow path member can be obtained.

  As shown in FIG. 6, two hook pins 13 f are formed on the lower surface side of the cover member 13. Further, the upper member 12 of the flow path member is formed with a hooking portion 13d for inserting the hooking pin 13f and the hooking pin 13f at the end, and the lower member 11 has an insertion hole for inserting the hooking pin 13f. 11c is formed. With such a configuration, the flow path member is manufactured by joining the lower member 11 and the upper member 12 by welding, and the analysis chip 10 is simply fitted into the flow path member later. Can be manufactured. Therefore, the shape of each member can be simplified and the manufacturability is also good, so that the production cost can be reduced.

  In addition, since the flow path member and the cover member 13 are separate bodies, the reagent fixed in the flow path 15 of the flow path member, the pretreatment pot 13a and the primary reaction treatment pot 13b of the cover member 13 are included. Since the reagent to be fixed can be generated in an environment having different conditions such as drying time and temperature, an optimal generation environment can be obtained for each.

  Further, a barcode BC representing predetermined information such as individual differences of the analysis chip 10 or the manufacturing date may be displayed on the surface of the analysis chip 10 such as the lower surface of the lower member 11. With such an aspect, it is possible to perform correction processing of errors due to individual differences of analysis chips, quality control, and the like on the measurement results obtained from the analysis chip 10 using the above information. Therefore, the merchantability of the analysis chip can be improved.

Next, a fluorescence detection apparatus that uses the analysis chip 10 of the present embodiment will be described.
FIG. 7 is a schematic diagram showing an example of a fluorescence detection apparatus using the analysis chip, FIG. 8 is a schematic configuration diagram of a measurement unit of the fluorescence detection apparatus, FIG. 9 is a block diagram of the fluorescence detection apparatus, and FIG. FIG. 11 is a schematic diagram showing how a sample is extracted from a sample container using a nozzle tip by the sample processing means of FIG. 9, and FIG. 12 is a diagram showing how the sample in the nozzle chip is extracted by the sample processing means of FIG. FIG. 13 is a schematic diagram showing an example of light irradiation means and fluorescence detection means in FIG. 9, and FIG. 14 is a graph showing quantitative or quantitative analysis by the rate method in the data analysis means in FIG. It is a graph which shows a mode that a qualitative analysis is performed.

  The fluorescence detection apparatus 1 is an immunological analysis apparatus using surface plasmon resonance, for example. When the analysis is performed by the fluorescence detection apparatus 1, as shown in FIGS. 7 and 8, the sample container CB in which the sample is accommodated, the nozzle chip NC used for extracting the sample and the reagent, and the analysis chip 10 include the table 61. Loaded on top. The sample container CB, the nozzle tip NC, the analysis chip 10 and the table 61 are all disposable items that are discarded once used. Then, the fluorescence detection apparatus 1 performs a quantitative or qualitative analysis on the test substance in the sample while flowing the sample through the microchannel 15 of the analysis chip 10.

  The fluorescence detection apparatus 1 includes a sample processing unit 20, a light irradiation unit 30, a fluorescence detection unit 40, a data analysis unit 50, and the like. The sample processing means 20 extracts a sample from the sample container CB containing the sample using the nozzle chip NC, and generates a sample solution obtained by mixing and stirring the extracted sample with a reagent.

  As shown in FIG. 8, the table 61 loaded with the sample container CB, the nozzle chip NC, and the analysis chip 10 moves from the loading position to a predetermined measurement position. At the measurement position, the dispensing unit 62 on which the sample processing means 20 is mounted and the measurement unit 63 on which the light irradiation means 30 and the fluorescence detection means 40 are mounted are sandwiched between the analysis chip 10 and the preprocessing pot 13a, The primary reaction processing pot 13b, the inlet insertion hole 13c, and the suction hole insertion hole 13d move in parallel with each other, and are configured to automatically execute predetermined processing described below. .

  When the start of analysis is instructed, the sample processing means 20 aspirates the sample from the sample container CB using the nozzle tip NC as shown in FIG. Thereafter, as shown in FIG. 12, the sample processing means 20 punctures the sealing member S of the preprocessing pot 13a and repeats the discharge and suction of the sample into the preprocessing pot 13a. After thoroughly mixing and stirring the reagent DC and the specimen, the specimen solution is again sucked using the nozzle tip NC. This operation is similarly performed for the primary reaction processing pot 13b. Then, a sample solution is generated in which the second antibody B2, which is a second binding substance specifically binding in the reagent, to the test substance (antigen) A present in the sample is modified on the surface. Then, the sample processing means 20 installs the nozzle tip NC containing the sample solution on the injection port 12a, and then creates a negative pressure in the suction hole 12b to cause the sample solution in the nozzle tip NC to be used for secondary reaction processing. It flows into the flow path 15.

  FIG. 13 is a schematic diagram showing an example of the light irradiation means 30 and the fluorescence detection means 40. In FIG. 13, the description will be given focusing on the test region TR, but the excitation light L is similarly applied to the control region CR. The light irradiation means 30 in FIG. 9 has the lower member 11 (dielectric plate) of the test region TR at an incident angle that causes the excitation light L to be totally reflected from the side surface side of the flow path member (lower member 11) of the analysis chip 10. ) And the metal film 16 are irradiated. The fluorescence detection means 40 is composed of, for example, a photodiode, a CCD, a CMOS, or the like, and detects fluorescence Lf generated from the test region TR by the irradiation of the excitation light L of the light irradiation means 30 as a fluorescence signal FS.

  Then, the excitation light L is incident on the interface between the lower member 11 (dielectric plate) and the metal film 16 at a specific incident angle equal to or greater than the total reflection angle by the light irradiating means 30. The evanescent wave Ew oozes out from the sample S, and surface plasmons are excited in the metal film 16 by the evanescent wave Ew. This surface plasmon causes an electric field distribution on the surface of the metal film 16 to form an electric field enhancement region. Then, the bound fluorescent labeling substance F is excited by the evanescent wave Ew and generates enhanced fluorescence.

  The data analysis means 50 in FIG. 9 analyzes the test substance based on the change over time of the fluorescence signal FS detected by the fluorescence detection means 40. Specifically, since the fluorescence intensity changes depending on the amount of the fluorescent labeling substance F bound thereto, the fluorescence intensity changes with time as shown in FIG. The data analysis means 50 acquires a plurality of fluorescent signals FS at a predetermined sampling period (for example, a period of 5 seconds) in a predetermined period (for example, 5 minutes), and analyzes the change rate of the fluorescence intensity over time, thereby analyzing the test within the sample. Perform quantitative analysis of substances (rate method). The analysis result is output from the information output means 4 comprising a monitor, a printer or the like.

  The preferred embodiments of the present invention have been described above, but it goes without saying that various improvements and modifications may be made without departing from the scope of the present invention.

DESCRIPTION OF SYMBOLS 1 Fluorescence detection apparatus 4 Information output means 10 Analysis chip 11 Lower member 12 Upper member 13 Cover member 13a Pretreatment pot 13b Primary reaction treatment pot 15 Channel 20 Sample processing means 30 Light irradiation means 40 Fluorescence detection means 50 Irradiation Position adjustment means 50 Data analysis means AS Unevenness BC Bar code CR Control area DS Dry reagent TR Test area

Claims (17)

An analysis chip used in a light detection method for detecting light generated from a photoresponsive labeling substance bonded to a test substance and analyzing the test substance,
A pot for storing the sample solution and performing a predetermined process on the sample solution;
A detection region for detecting light generated from the photoresponsive labeling substance, and a flow path through which the sample solution flows,
An inlet provided on the upstream side of the flow path, for injecting the sample solution into the flow path;
A suction hole provided on the downstream side of the flow path, for sucking the sample solution injected from the injection port from the downstream side;
The flow path is formed, and at least the light path portion of the light with respect to the detection region has a light-transmitting flow path member,
An analysis chip comprising the pot and a cover member fitted to the flow path member.   The detection region includes a dielectric plate for entering excitation light for generating an evanescent wave, and a metal layer provided in a predetermined region on the specimen solution contact surface side of the dielectric plate. The analysis chip according to claim 1.   The analysis chip according to claim 1, wherein the pot includes a pretreatment pot for performing a predetermined pretreatment on the sample solution.   The analysis according to any one of claims 1 to 3, wherein the pot includes a primary reaction processing pot for binding a test substance and a photoresponsive labeling substance in the sample solution. Chip.   The analysis chip according to claim 3 or 4, wherein a predetermined dry reagent is fixed to the inner surface of at least one of the pretreatment pot and the primary reaction treatment pot.   6. The analysis chip according to claim 5, wherein irregularities for preventing the dry reagent from being peeled are formed in the dry reagent fixing portion.   The analysis chip according to claim 5 or 6, wherein an opening of at least one of the pretreatment pot and the primary reaction treatment pot is sealed.   The analysis chip according to claim 1, wherein the inlet, the suction hole, and the pot are arranged in a straight line.   The analysis chip according to any one of claims 1 to 8, wherein the detection area includes a plurality of detection areas arranged in a straight line. A lower member and an upper member, wherein the flow path is formed between the lower member and the upper member, and at least an optical path portion of light with respect to the detection region is a light-transmitting flow path member ,
The analysis chip according to claim 1, wherein the analysis chip according to claim 1 is configured by a cover member that is formed with the pot and is fitted from the upper member side of the flow path member.   A contact portion of a horn for ultrasonic welding the lower member and the upper member is flat on at least one of the lower surface of the lower member and the upper surface of the upper member. The analysis chip according to any one of claims 1 to 10.   The analysis chip according to claim 1, wherein the cover member is provided with an opening in a region facing the light detection region.   The analysis chip according to any one of claims 1 to 12, wherein a bar code representing predetermined information is displayed on a surface of at least one of the flow path member and the cover member.   The analysis chip according to any one of claims 1 to 13, wherein the suction hole, the pot, and the injection port are linearly arranged in this order. The pot includes a pretreatment pot for performing a pretreatment before performing a primary reaction treatment for binding a photoresponsive labeling substance and a test substance,
The analysis chip according to claim 1, wherein the suction hole, the pretreatment pot, and the injection port are linearly arranged in this order. The pot includes a primary reaction processing pot that contains a photoresponsive labeling substance and performs a primary reaction process for binding the photoresponsive labeling substance and a test substance,
The analysis chip according to any one of claims 1 to 14, wherein the suction hole, the pot for primary reaction processing, and the injection port are arranged linearly in this order. As the pot, a pretreatment pot for performing a pretreatment before performing a primary reaction treatment for binding a photoresponsive labeling substance and a test substance, and a photoresponsive labeling substance are accommodated in the pot. A primary reaction treatment pot for performing a primary reaction treatment for binding a labeling substance and a test substance;
The analysis chip according to any one of claims 1 to 14, wherein the suction hole, the pretreatment pot, the primary reaction treatment pot, and the injection port are arranged linearly in this order. .