JP5677342B2 - Pili movement activator - Google Patents

Description

The present invention aims to enhance the ability to transport and discharge mucus produced from the respiratory tract as a ciliary movement activator, and as a result, enhance the biological defense ability against bacteria, foreign substances and the like inhaled into the body. those related to the motion activator.

  In recent years, the airway is chronically damaged due to an increase in chemical substances exposed to air pollution and the respiratory tract, and the number of people complaining of difficulty in hemorrhoids due to a decrease in the ability of the airway epithelium to discharge is increasing. Especially in elderly people, sputum and swallowing ability are reduced, which is a big problem.

  In particular, the trachea is constantly exposed to foreign objects such as particles, gas, dust, bacteria and viruses. In order to deal with these foreign substances, the airway mucosal epithelium located at the outermost surface of the trachea has a function of discharging foreign substances, protects the airway cavity, and preserves normal respiratory function. The airway mucosal epithelium is composed of various types of cells, of which the most important are goblet cells that produce mucus and pilus cells that have pili. When foreign substances such as particles, gas, dust, bacteria, and viruses reach the trachea, mucus produced from goblet cells catches the foreign substances, and active movement by the cilia possessed by the ciliated cells (hereinafter, cilia) It is carried to the pharynx by movement) and processed by extracorporeal discharge or swallowing as a sputum.

  The relationship between cilia movement and mucus transport is derived from the hydrodynamic viewpoint by the formula (mucus transport speed = mucus density x cilia movement speed x cilia length / viscosity), and when cilia movement becomes active, mucus transport Is thought to increase.

  Until now, cough and sputum have been seen as negative symptoms in medical practice. In particular, for coughs, antitussives were easily administered as a cause of respiratory problems and physical exhaustion, and treatments to suppress cough, which is a physiological reaction for foreign body excretion, were often taken. However, the idea that it is preferable to promote the discharge of physiological sputum and, as a result, calm the cough, instead of stopping the cough itself hard.

  Conventionally, as a method for promoting the discharge of sputum, a method of lowering the consistency of sputum by dissolving airway mucus has been used, and airway mucus solubilizers such as carbocysteine and bromhexine hydrochloride have been used (for example, patent documents). 1). However, side effects such as rash, eczema, diarrhea, and abdominal pain have been reported to occur in the airway mucus solubilizing agent itself, which is a chemically synthesized product (see Non-Patent Document 1, for example).

  It is known that the mechanism of action is different from that of the above airway mucus solubilizer, and substances that activate ciliary movement of the airway epithelium also have an effect of enhancing foreign matter excretion ability by enhancing mucus transport. Searches for extracts having a ciliary motility activation effect from plants that are natural products from the viewpoint that there are few problems with safety are jujube (see, for example, Non-Patent Document 2), black beans (for example, non-patent) However, an extract having a sufficient effect of activating ciliary movement has not been obtained yet.

JP-A-8-337532

Pocket Medicine Collection (2001 edition), Hakubunsha, p. 362 Experimental Lung Research, 22, p. 255-266, 1996 Monthly food chemical p. 88-89, April 2001 issue

The present invention has been made in order to solve the above-described problems of the prior art, and an object thereof is to provide a safe and highly effective ciliary movement activator.

In order to solve the above-mentioned problems, the present inventors conducted extensive screening for substances exhibiting a ciliary motility activation effect, and as a result, extracted with water or an alcohol solvent for the temporary seed coat of myrtle (Myristica fragrans) . The present inventors have found that the extract has a ciliary motility activation effect and have completed the present invention.

That is, the present invention is a ciliary motility activator comprising, as an active ingredient, an extract extracted from water or an alcohol solvent with respect to a temporary seed coat of myrtle (Myristica fragrans).

  The ciliary motility activator of the present invention uses menthol, menthol derivatives, menthol isomers, etc., which have been used in herbal medicines and folklore for a long time, and / or menthol derivatives generally used in foods and drinks. Therefore, there is no problem in terms of safety and excellent taste. Therefore, the ciliary movement activator of the present invention and foods and drinks containing the same are not accompanied by side effects such as airway mucus solubilizers composed of chemically synthesized products, and infectious diseases derived from foreign bacteria, viruses, foreign substances, etc. Diseases (bronchitis, bronchiectasis, sinusitis, bronchial asthma, pneumonia, etc.) can be prevented.

  Plants used in the present invention, Musaceae Musa plant, Myristica fragrans, Rutaceae Citrus plant, Skunk cabbage (Symplocarpus foetidus), Sekisho (Acorus gramineus), Prickly ash ( Zanthoxylum americanum, Lycium chinense, Nettle (Urtica dioica), Lotus (Nelumbo nucifera), Sambucus nigra, Sasa veitchii, and Crataeegus oxyacantha You can use parts such as germ, endocarp, outer pericarp, mature fruit, immature fruit, pericarp, seed coat, temporary seed coat, flower, wood, bark, root, rhizome, etc. For skunk cabbage, rhizomes, for roots, roots for prickly ash, bark, wolfberry, neto It is preferred to use leaves for le and kumaza, germs for lotus, flowers for elderberry, and fruit for hawthorn.

  As long as it is included in the genus of the genus Baxaceae, the type of the plant is not particularly limited. Is preferred. Moreover, it is preferable to use a fruit and pericarp for the plant part for extraction in the genus Bacillus.

  As for the mandarin orange genus plants, the type of the plant is not particularly limited as long as it is included in this species, but Daidai (Citrus aurantium), Citrus kinokuni, Navel orange (Citrus sinensis), Lemon (Citrus limon) ), It is preferable to use Nippon tachibana. Moreover, it is preferable to use a fruit, a skin, a flower, and a leaf about the plant site | part for providing to extraction in the citrus genus Citrus.

  As a ciliary movement activator of this invention, you may contain each plant as an active ingredient as it is, but you may contain what dried and also powdered the dried material as an active ingredient. Moreover, you may contain the extract extracted from each plant as an active ingredient. The plant extract may be a liquid extract extracted with various solvents, but the liquid extract is further solidified or concentrated by an ordinary drying method (for example, reduced pressure drying, freeze drying, etc.) or a concentration method. It may be what you did. The type of the solvent is not particularly limited. In addition to water (hot water), alcohols such as ethanol, methanol, propanol, glycerin and propylene glycol, organic solvents such as ether, acetone, ethyl acetate, chloroform and hexane or these Solvents mixed as appropriate can be used, but taking into account the case of oral ingestion as a ciliary movement activator, extraction from water, ethanol or a mixed solution thereof is desirable from the viewpoint of safety. Furthermore, you may perform refinement | purification processes, such as deodorizing and decoloring, to this plant extract in the range which does not affect the effect as needed.

  The extraction condition for obtaining the extract from the plant is not particularly limited, but is preferably about 1 to 5 hours at 50 to 100 ° C. The extract can be further filtered and the extract solvent is distilled off, and then concentrated or lyophilized under reduced pressure. Further, those obtained by fractionating and purifying these extracts by organic solvent fractionation, column chromatography or the like can also be used.

  Menthol, menthol derivatives and menthol isomers used in the present invention include, but are not limited to, L-menthol, D-menthol, menthyl acetate, isomenthol and the like. Menthol, menthol derivatives and menthol isomers may be encapsulated (microencapsulated) or encapsulated with cyclodextrin or the like. Moreover, you may use the essential oil containing these, a blended fragrance | flavor, etc., for example, it is also possible to use essential oils, such as peppermint oil (light-loading oil).

  The ciliary motility activator of the present invention is dissolved or dispersed in an appropriate liquid alone, if necessary, mixed with an appropriate powder alone or adsorbed thereto, and in some cases, an emulsifier, Add additives, dispersants, flavoring agents, preservatives, fragrances, coloring agents, coating agents, etc., as desired tablets, liquids, injections, ointments, creams, lotions, sprays, aerosols, suppositories, etc. The dosage form may be used as oral preparations, external preparations, injections, inhalants, nasal drops, eye drops and the like. In this case, it is suitable to use 0.001% by weight or more, preferably 0.01% by weight or more of the plant extract and / or compound with respect to the agent.

  Further, the ciliary movement activator of the present invention is excellent in fragrance, taste, and safety, so it is a confectionery such as chewing gum, candy, tablet confectionery, gummy jelly, chocolate, biscuits, ice cream, sorbet, ice confectionery. It can be used on a daily basis by blending it into food and drink such as frozen desserts, beverages, soups, jams and the like.

  The amount of ciliary movement activator added to the food or drink of the present invention is about 0.001 to 5% by weight, preferably about 0.01 to 3% by weight, especially considering the aspect of palatability. It is suitable to add to.

  Musaceae Musa plant, Myristica fragrans, Rutaceae Citrus plant, Skunk cabbage (Symplocarpus foetidus), Sekisho (Acorus gramineus), Prickly american um (Zanthoxylum american) used in the present invention ), Lycium chinense, Nettle (Urtica dioica), Lotus (Nelumbo nucifera), Sambucus nigra, Sasa veitchii, and Crataegus oxyacantha are all herbal medicines, food ingredients, herbal teas It has been used for a long time, and there is no problem at all about the safety of these extracts and foods and drinks containing them. In addition, since menthol, menthol derivatives and menthol isomers are generally blended in foods and drinks, there is no safety problem.

  Hereinafter, the present invention will be specifically described with reference to test examples, but the present invention is not limited thereto.

Test example 1

  This test was conducted to examine the effect of pilus motility activation of plant extracts obtained from plants.

1) Test sample A plant extract was prepared by the method described below.

[Sample Preparation Example 1]
100 ml of 50% ethanol was added to 10 g each of dried and crushed rye banana peel, nutmeg temporary seed coat, prickly ash bark, kumaza leaf, and hawthorn berries, and refluxed at 70 ° C. for 2 hours. . Each obtained extract was individually filtered, concentrated and freeze-dried to obtain a ciliary motility activator according to the present invention. 2.1 g of nutmeg extract, 4.2 g of licorice banana extract, 0.6 g of prickly ash extract, 0.7 g of kumazasa extract, and 1.3 g of hawthorn extract were obtained.

[Sample Preparation Example 2]
100 ml of water was added to 10 g each of dried and crushed banana peel, nutmeg temporary seed coat, prickly ash bark, kumaza leaf, and hawthorn berry, and refluxed at 70 ° C. for 2 hours. Each obtained extract was individually filtered, concentrated and freeze-dried to obtain a ciliary motility activator according to the present invention. The nutmeg extract was 2.1 g, the banana extract was 2.8 g, the prickly ash extract was 0.5 g, the kumazasa extract was 0.7 g, and the hawthorn extract was 0.9 g.

[Sample Preparation Example 3]
100 ml of 50% ethanol was added to 10 g each of dried and crushed skunk cabbage rhizome, buffalo root, sunflower seed, lotus germ, and elderflower, followed by reflux extraction at 70 ° C. for 2 hours. Each obtained extract was individually filtered, concentrated and freeze-dried to obtain a ciliary motility activator according to the present invention. As a result, 2.3 g of the skunk cabbage extract, 0.6 g of the birch extract, 4.0 g of the Malayan bay pepper extract, 2.4 g of the lotus extract, and 2.3 g of the elderberry extract were obtained.

[Sample Preparation Example 4]
Add 100 ml of water to 10 g each of dried and crushed skunk cabbage rhizome, pebbles root, banana fruit, lotus germ, elderflower flower, citrus peel and lemon leaf, and reflux at 70 ° C. for 3 hours. Extraction was performed. The obtained extract was individually filtered, concentrated, and lyophilized to obtain a ciliary motility activator according to the present invention. The skunk cabbage extract is 2.3 g, the biloba extract is 1.5 g, the banana extract is 4.0 g, the lotus extract is 2.7 g, the elderberry extract is 2.1 g, and the citrus orange extract is 3.1 g. 2.6 g of lemon extract was obtained.

[Sample Preparation Example 5]
300 ml of 50% ethanol was added to 30 g each of dried and crushed navel orange flowers, wolfberry leaves and nettle leaves and extracted at 90 ° C. for 1 hour. Each obtained extract was individually filtered, concentrated and freeze-dried to obtain a ciliary motility activator according to the present invention. 6.9 g of navel orange extract, 8.4 g of wolfberry extract, and 3.3 g of nettle extract were obtained.

[Sample Preparation Example 6]
300 ml of water was added to 30 g of each dried and crushed Nippon Tachibana fruit, wolfberry leaf, and nettle leaf, and extracted at 90 ° C. for 1 hour. The obtained extract was filtered, concentrated, and lyophilized to obtain a ciliary motility activator according to the present invention. 7.8 g of Nippon Tachibana extract, 8.4 g of wolfberry extract, and 5.4 g of nettle extract were obtained.

[Sample Preparation Example 7]
100 g of 100% methanol was added to 10 g each of dried and crushed rye banana peel and nutmeg temporary seed coat and extracted at 70 ° C. for 3 hours. The obtained extract was filtered, concentrated and dried under reduced pressure to obtain a ciliary motility activator according to the present invention. 1.0 g of ryori banana extract and 1.4 g of nutmeg extract were obtained.

[Sample Preparation Example 8]
100 ml of 100% ethanol was added to 10 g each of dried and crushed banana peels, red rhizomes, and Daidai fruits, and extracted at 70 ° C. for 3 hours. The obtained extract was filtered, concentrated and dried under reduced pressure to obtain a ciliary motility activator according to the present invention. As a result, 1.2 g of banana extract, 0.5 g of pepper extract, and 1.9 g of Daidai extract were obtained.

[Comparative sample 1]
300 ml of water was added to 30 g of dried and crushed jujube fruits, followed by reflux extraction at 90 ° C. for 1 hour. The obtained extract was filtered, concentrated and freeze-dried to obtain 15.3 g of jujube extract.

[Comparative sample 2]
300 ml of water was added to 30 g of dried and crushed blackberry fruits, followed by reflux extraction at 70 ° C. for 2 hours. The obtained extract was filtered, concentrated and freeze-dried to obtain 11.1 g of a black bean extract.

2) Test method Using the ciliary motility activator of the present invention shown in Sample Preparation Examples 1 to 8 and the comparative extract shown in Comparative Examples 1 and 2 as samples, the ciliary motility activation effect was examined.

  This test measures the ciliary movement of ciliated cells extracted from the rabbit trachea and confirms the effect of the plant extract activating ciliary movement.

A Japanese white rabbit (2.0-2.3 kg) was anesthetized with pentobarbital sodium, the trachea was removed, the airway mucosa epithelium containing ciliated cells was peeled from the trachea, and cut into small pieces of 1-3 mm ² . Thereafter, it was placed on a cover glass in a petri dish filled with Medium 199 (containing penicillin 50 U / ml, streptomycin 50 μg / ml, fungizone 2.5 μg / ml).

Cultivation was performed for 7 days in a CO ₂ incubator (95% air, 5% CO ₂ ) under 37 ° C conditions. The tissue was affixed to the gas phase surface of a cover glass inclined at about 30 degrees, and the culture was continued with the petri dish horizontal after 48 hours. Next, this was turned upside down and fixed in a rose chamber filled with Medium 199, and the ciliary motion frequency (hereinafter abbreviated as “CBF”) was measured by the microphotooscillation method (Okazawa Mitsushiba et al. Respiration 5: 1345-1350, 1986).

  The measurement was carried out in a constant temperature bath at 37 ° C. First, permeate the rose chamber with Medium 199 and measure CBF continuously for 15 minutes, then perfuse CBF at almost the same site with Sample 199 and measure CBF continuously for 15 minutes. Calculation was performed according to the following formula.

Ciliary motor activation rate (%) = (A / B-1) × 100
However, A: Average value of CBF for 15 minutes after perfusion with Sample-added Medium 199 B: Average value of CBF for 15 minutes after perfusion with Medium 199 (basic value)

3) Test results Table 1 shows the activation rate of ciliary motility of each sample at 1 mg / ml.

  From the results shown in Table 1, the present invention bananas, leopard bananas, nutmeg, malaysian mackerel, prickly ash, skunk cabbage, red pepper, daidai, kishu mikan, navel orange, lemon, nippon tachibana, wolfberry, nettle, lotus, elderberry The extracts prepared from crisp, Kumazasa, and Hawthorn showed strong ciliary motility activation, but the circadian motility activation of jujube extract and black bean extract, which are comparative examples, was weak. It was.

Test example 2

This test was conducted to examine the effect of menthol, menthol derivatives and menthol isomers on ciliary motility activation.
1) Test samples L-menthol, D-menthol, isomenthol and menthyl acetate were used.
2) Test method This test was conducted by the same test method as in Test Example 1.
3) Test results Table 2 shows the activation rate of ciliary movement of each sample at 50 μg / ml.

  From the results of Table 2, L-menthol, D-menthol, isomenthol, and menthyl acetate all showed a strong ciliary motility activation effect.

  EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples, but the present invention is not limited to these examples.

  Using the plant extract prepared in Sample Preparation Examples 1 to 8, plant powder prepared by crushing dried plants, menthol, menthol derivatives and menthol isomers, tablets, powders, inhalants, nasal drops, chewing gum, Candy, chocolate, biscuits, gummy jelly, tablet confectionery, ice cream, sherbet, and beverage were prepared by conventional methods. The prescription is shown below.

Tablets were prepared according to the following formulation.
D-mannitol 42.6%
Lactose 42.6
Crystalline cellulose 8.5
Hydroxypropylcellulose 4.3
Skunk Cabbage Extract 2.0 of Sample Preparation Example 2.0
100.0%

  Tablets containing the extracts of Sample Preparation Example 1 to Sample Preparation Example 8 in the same manner as described above were prepared in the same manner instead of the skunk cabbage extract. These had a ciliary movement activation effect without impairing the properties as a tablet.

A powder was prepared according to the following formulation.
Lactose 62.5%
Potato starch 25.0
Nettle extract of sample preparation example 6 12.5
100.0%

  Powders containing the extracts of Sample Preparation Example 1 to Sample Preparation Example 8 were prepared in the same manner in the same mixing ratio as above, instead of the nettle extract. These had a ciliary movement activation effect without impairing the properties as a powder.

An inhalant was prepared according to the following formulation.
Ethanol 5.0%
Prickly ash extract of Sample Preparation Example 1 0.5
Navel orange extract of Sample Preparation Example 5 0.5
L-Menthol 2.0
Water 92.0
100.0%

  Inhalants were prepared in the same manner with the same blending ratios as above, but blended with each extract of Sample Preparation Example 1 to Sample Preparation Example 8 instead of Prickly Ash Extract and Navel Orange Extract. These had a ciliary movement activation effect without impairing the properties as an inhalant.

Nasal drops were prepared according to the following prescription.
Methyl salicylate 0.03g
Chlorpheniramine maleate 0.3
dl-methylephedrine hydrochloride 0.3
Polysorbate 80 0.2
Benzalkonium chloride 0.01
Sodium chloride 0.6
1N sodium hydroxide appropriate amount Sample extract 2 of sample preparation 1.0
Appropriate amount of water
100.0 ml (pH 6.5)

  Nasal drops containing the extracts of Sample Preparation Example 1 to Sample Preparation Example 8 were prepared in the same manner in the same mixing ratio as above. These had a ciliary motility activation effect without impairing the properties as nasal drops.

Chewing gum was prepared according to the following formulation.
Gum base 20.0%
Sugar 54.7
Glucose 15.0
Minamata 9.3
Fragrance 0.5
Banana extract of Sample Preparation Example 2 0.5
100.0%

  In the same blending ratio as above, chewing gums blended with the extracts of Sample Preparation Example 1 to Sample Preparation Example 8 instead of the banana extract were similarly prepared. These had a ciliary movement activation effect without impairing the properties as chewing gum.

Chewing gum was prepared according to the following formulation.
Gum base 20.0%
Sugar 54.5
Glucose 10.0
Minamata 13.0
Fragrance 0.5
Banana extract of Sample Preparation Example 4 1.0
The extract of sample preparation 4 was 1.0.
100.0%

  Chewing gums containing the extracts of Sample Preparation Example 1 to Sample Preparation Example 8 in the same manner as described above were prepared in the same manner in place of the banana extract and the pepper extract. These had a ciliary movement activation effect without impairing the properties as chewing gum.

Chewing gum was prepared according to the following formulation.
Gum base 20.0%
Sugar 50.5
Glucose 14.0
Minamata 13.0
Fragrance 0.5
Die-dye extract of Sample Preparation Example 2.0
100.0%

  In the same blending ratio as above, chewing gums blended with the extracts of Sample Preparation Example 1 to Sample Preparation Example 8 instead of Daidai Extract were similarly prepared. These had a ciliary movement activation effect without impairing the properties as chewing gum.

Candy was prepared according to the following formulation.
Sugar 50.0%
Minamata 33.0
Citric acid 1.0
Fragrance 0.2
L-Menthol 1.0
Banana extract of Sample Preparation Example 2 0.4
Water 14.4
100.0%

  Candy in which the extracts of Sample Preparation Example 1 to Sample Preparation Example 8 were mixed in the same manner as described above instead of the banana extract was prepared in the same manner. These had a ciliary movement activation effect without impairing the properties as candy.

Candy was prepared according to the following formulation.
Sugar 38.0%
Minamata 41.0
Citric acid 1.0
Fragrance 0.2
Powdered banana (skin) 1.4
Water 18.4
100.0%

  Candy in which powdered nutmeg and / or powdered lemon was blended instead of powdered banana (skin) at the same blending ratio as above was similarly prepared. These had a ciliary movement activation effect without impairing the properties as candy.

Candy was prepared according to the following formulation.
Sugar 38.0%
Minamata 32.0
Xylitol 10.0
Citric acid 1.0
Fragrance 0.2
Chrysanthemum extract of Sample Preparation Example 4 0.4
Water 18.4
100.0%

  Candy in which each of the extracts of Sample Preparation Example 1 to Sample Preparation Example 8 was mixed in the same manner as above was used in the same manner. These had a ciliary movement activation effect without impairing the properties as candy.

Chocolate was prepared according to the following formulation.
Cocoa bitter 20.0%
Whole milk powder 20.0
Cocoa butter 17.0
Powdered sugar 41.85
Lecithin 0.45
Fragrance 0.1
Lotus extract of Sample Preparation Example 4 0.6
100.0%

  The chocolate which mix | blended each extract of sample preparation example 1 thru | or sample preparation example 8 instead of the lotus extract was similarly prepared by the same mixing | blending ratio as the above. These had a ciliary movement activation effect without impairing the properties as chocolate.

Biscuits were prepared according to the following recipe.
31.7% sugar
Flour 26.8
Starch flour 26.8
Butter 3.2
Egg 10.7
Baking soda 0.3
Ryoori banana extract of Sample Preparation Example 1 0.5
100.0%

  Biscuits in which the extracts of Sample Preparation Example 1 to Sample Preparation Example 8 were mixed in the same manner in the same mixing ratio as above were used instead of the ryoli banana extract. They had a ciliary movement activation effect without impairing the properties as biscuits.

Gummy jelly was prepared according to the following formulation.
Polydextrose aqueous solution 40.0%
Sorbitol aqueous solution 8.0
Palatinose aqueous solution 9.0
Maltose aqueous solution 20.0
Trehalose aqueous solution 11.0
Gelatin 10.0
Tartaric acid 1.0
The extract of sample preparation 8 was 1.0.
100.0%

  Gummy jelly blended with the extracts of Sample Preparation Example 1 to Sample Preparation Example 8 in the same manner in place of the pepper extract was prepared in the same manner. These had ciliary motility activation effect without impairing the properties of gummy jelly.

Tablet confectionery was prepared according to the following formulation.
76.1% sugar
Glucose 19.0
Sucrose fatty acid ester 0.2
Fragrance 0.2
Ryori banana extract of Sample Preparation Example 7 0.5
Water 4.0
100.0%

  Tablets containing the extracts of Sample Preparation Example 1 to Sample Preparation Example 8 in the same manner as described above were prepared in the same manner in place of the ryoli banana extract. These had a ciliary movement activation effect without impairing the properties as a tablet confectionery.

Tablet confectionery was prepared according to the following formulation.
74.7% sugar
Lactose 18.9
Sucrose fatty acid ester 0.15
Hawthorn extract of sample preparation example 1 1.0
Lemon extract of sample preparation example 4 1.0
Water 4.25
100.0%

  Tablets containing the extracts of Sample Preparation Example 1 to Sample Preparation Example 8 instead of hawthorn extract and lemon extract were prepared in the same manner at the same mixing ratio as above. These had a ciliary movement activation effect without impairing the properties as a tablet confectionery.

Ice cream was prepared according to the following formulation.
Yolk 11.0%
Sugar 14.0
Milk 37.0
Fresh cream 37.0
Vanilla beans 0.5
Kumazasa Extract of Sample Preparation Example 2 0.5
100.0%

  Ice creams containing the extracts of Sample Preparation Example 1 to Sample Preparation Example 8 were prepared in the same manner in the same mixing ratio as above, instead of Kumazasa extract. These had a ciliary movement activation effect without impairing the properties of ice cream.

A sherbet was prepared according to the following formulation.
Orange juice 16.0%
Sugar 32.0
Malayan bay pepper extract of Sample Preparation Example 3 2.0
Water 50.0
100.0%

  The sherbet which mix | blended each extract of the sample preparation example 1 thru | or the sample preparation example 8 instead of the Malayan bay shoot extract was similarly prepared with the same mixture ratio as the above. These had a ciliary movement activation effect without impairing the properties as a sherbet.

A beverage was prepared according to the following formulation.
Orange juice 30.0%
Isomerized sugar 15.24
Citric acid 0.1
Vitamin C 0.04
Fragrance 0.1
Wolfberry extract of sample preparation example 6 0.1
Water 54.42
100.0%

  Beverages blended with the extracts of Sample Preparation Example 1 to Sample Preparation Example 8 instead of the wolfberry extract were prepared in the same manner at the same blending ratio as above. These had a ciliary movement activation effect without impairing the properties as a beverage.

Tablets were prepared according to the following formulation.
D-mannitol 43.0%
Lactose 43.0
Crystalline cellulose 9.0
Hydroxypropylcellulose 4.0
L-Menthol 1.0
100.0%

  Tablets containing any of D-menthol, menthyl acetate and isomenthol instead of L-menthol were prepared in the same manner at the same mixing ratio as above. These had a ciliary movement activation effect without impairing the properties as a tablet.

A powder was prepared according to the following formulation.
Lactose 67.5%
Potato starch 30.0
L-Menthol 2.5
100.0%

  Powders containing any of D-menthol, menthyl acetate and isomenthol instead of L-menthol were prepared in the same manner at the same mixing ratio as above. These had a ciliary movement activation effect without impairing the properties as a powder.

An inhalant was prepared according to the following formulation.
Ethanol 5.0%
L-Menthol 1.5
D-Menthol 1.5
Water 92.0
100.0%

  Inhalants containing either menthyl acetate or isomenthol instead of L-menthol and D-menthol were prepared in the same manner at the same blending ratio as above. These had a ciliary movement activation effect without impairing the properties as an inhalant.

Chewing gum was prepared according to the following formulation.
Gum base 20.0%
Sugar 55.0
Glucose 15.0
Minamata 9.4
Fragrance 0.5
L-Menthol 0.1
100.0%

  In the same blending ratio as above, chewing gums blended with any of D-menthol, menthyl acetate and isomenthol instead of L-menthol were prepared in the same manner. These had a ciliary movement activation effect without impairing the properties as chewing gum.

Candy was prepared according to the following formulation.
Sugar 39.0%
Minamata 41.2
Citric acid 1.0
Fragrance 0.2
Menthyl acetate 0.1
Water 18.5
100.0%

  Candy in which any one of L-menthol, D-menthol, and isomenthol was blended in place of menthyl acetate at the same blending ratio was prepared in the same manner. These had a ciliary movement activation effect without impairing the properties as candy.

Candy was prepared according to the following formulation.
Sugar 38.0%
Minamata 32.0
Xylitol 10.0
Citric acid 1.0
Fragrance 0.2
L-Menthol 0.2
Menthyl acetate 0.2
Water 18.4
100.0%

  Candy in which any of D-menthol and isomenthol was blended in place of L-menthol and menthyl acetate at the same blending ratio was prepared in the same manner. These had a ciliary movement activation effect without impairing the properties as candy.

A beverage was prepared according to the following formulation.
Orange juice 30.00%
Isomerized sugar 15.26
Citric acid 0.10
Vitamin C 0.07
Fragrance 0.10
Menthyl acetate 0.05
Water 54.42
100.0%

  Beverages blended with any of L-menthol, D-menthol, and isomenthol instead of menthyl acetate at the same blending ratio as above were prepared in the same manner. These had a ciliary movement activation effect without impairing the properties as a beverage.

Claims (1)

A ciliary motility activator comprising, as an active ingredient, an extract extracted from water or an alcohol solvent for a temporary seed coat of myrtle (Myristica fragrans).