JP5224469B2 - Method for producing vegetable extract - Google Patents
Method for producing vegetable extract Download PDFInfo
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- JP5224469B2 JP5224469B2 JP2009131268A JP2009131268A JP5224469B2 JP 5224469 B2 JP5224469 B2 JP 5224469B2 JP 2009131268 A JP2009131268 A JP 2009131268A JP 2009131268 A JP2009131268 A JP 2009131268A JP 5224469 B2 JP5224469 B2 JP 5224469B2
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- 235000013311 vegetables Nutrition 0.000 title claims description 64
- 238000004519 manufacturing process Methods 0.000 title claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 63
- 102000004190 Enzymes Human genes 0.000 claims description 56
- 108090000790 Enzymes Proteins 0.000 claims description 56
- 238000011282 treatment Methods 0.000 claims description 40
- 150000007524 organic acids Chemical class 0.000 claims description 37
- 108010059820 Polygalacturonase Proteins 0.000 claims description 28
- 206010033546 Pallor Diseases 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 19
- -1 organic acid salt Chemical class 0.000 claims description 15
- 229940088598 enzyme Drugs 0.000 description 55
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 39
- 239000002994 raw material Substances 0.000 description 26
- 239000007788 liquid Substances 0.000 description 21
- 230000000694 effects Effects 0.000 description 19
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 18
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 16
- 101710130006 Beta-glucanase Proteins 0.000 description 16
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 16
- 102100022624 Glucoamylase Human genes 0.000 description 16
- 230000001603 reducing effect Effects 0.000 description 14
- 235000015165 citric acid Nutrition 0.000 description 13
- 239000000047 product Substances 0.000 description 12
- 229960005070 ascorbic acid Drugs 0.000 description 9
- 235000013361 beverage Nutrition 0.000 description 9
- 238000005406 washing Methods 0.000 description 9
- 108010093305 exopolygalacturonase Proteins 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 238000000926 separation method Methods 0.000 description 8
- 102000004400 Aminopeptidases Human genes 0.000 description 7
- 108090000915 Aminopeptidases Proteins 0.000 description 7
- 102000004139 alpha-Amylases Human genes 0.000 description 7
- 108090000637 alpha-Amylases Proteins 0.000 description 7
- 229940024171 alpha-amylase Drugs 0.000 description 7
- 238000004659 sterilization and disinfection Methods 0.000 description 7
- 241001075517 Abelmoschus Species 0.000 description 6
- 238000004140 cleaning Methods 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 6
- 238000011156 evaluation Methods 0.000 description 6
- 239000011668 ascorbic acid Substances 0.000 description 5
- 229940093915 gynecological organic acid Drugs 0.000 description 5
- 235000005985 organic acids Nutrition 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 235000015192 vegetable juice Nutrition 0.000 description 5
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 4
- 239000002211 L-ascorbic acid Substances 0.000 description 4
- 235000000069 L-ascorbic acid Nutrition 0.000 description 4
- 108091005804 Peptidases Proteins 0.000 description 4
- 239000004365 Protease Substances 0.000 description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 4
- 235000010323 ascorbic acid Nutrition 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 3
- 241000447437 Gerreidae Species 0.000 description 3
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 3
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 238000006911 enzymatic reaction Methods 0.000 description 3
- 235000013373 food additive Nutrition 0.000 description 3
- 239000002778 food additive Substances 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 239000001630 malic acid Substances 0.000 description 3
- 235000011090 malic acid Nutrition 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 239000011975 tartaric acid Substances 0.000 description 3
- 235000002906 tartaric acid Nutrition 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 2
- 229960005069 calcium Drugs 0.000 description 2
- 235000001465 calcium Nutrition 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 235000015203 fruit juice Nutrition 0.000 description 2
- 239000001530 fumaric acid Substances 0.000 description 2
- 235000011087 fumaric acid Nutrition 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 239000003002 pH adjusting agent Substances 0.000 description 2
- 238000012805 post-processing Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 230000001953 sensory effect Effects 0.000 description 2
- 235000002639 sodium chloride Nutrition 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 2
- 239000011345 viscous material Substances 0.000 description 2
- WVXRAFOPTSTNLL-NKWVEPMBSA-N 2',3'-dideoxyadenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1CC[C@@H](CO)O1 WVXRAFOPTSTNLL-NKWVEPMBSA-N 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 240000003394 Malpighia glabra Species 0.000 description 1
- 235000014837 Malpighia glabra Nutrition 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 235000011054 acetic acid Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229920001284 acidic polysaccharide Polymers 0.000 description 1
- 150000004805 acidic polysaccharides Chemical class 0.000 description 1
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 235000013734 beta-carotene Nutrition 0.000 description 1
- 239000011648 beta-carotene Substances 0.000 description 1
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 description 1
- 229960002747 betacarotene Drugs 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Chemical class 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 229960003975 potassium Drugs 0.000 description 1
- 235000007686 potassium Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium ascorbate Substances [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 description 1
- 235000010378 sodium ascorbate Nutrition 0.000 description 1
- 229960005055 sodium ascorbate Drugs 0.000 description 1
- PPASLZSBLFJQEF-RXSVEWSESA-M sodium-L-ascorbate Chemical compound [Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RXSVEWSESA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 1
Landscapes
- Preparation Of Fruits And Vegetables (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Description
本発明は、モロヘイヤやオクラ、ツルムラサキなど、ぬめり成分を含有する野菜から搾汁成分としての野菜エキスを製造する方法に関する。 The present invention relates to a method for producing a vegetable extract as a squeezed component from a vegetable containing a slime component, such as Morohaya, Okra, and Tsurumurasaki.
モロヘイヤの葉及び茎には、カリウム、カルシウム等のミネラル、βカロチン及びビタミン類が豊富に含まれているほか、例えばグルコースやアラビノースなど、血中コレステロールや中性脂肪を低下させる粘液多糖類も含まれている。このように、モロヘイヤには体に良いとされる成分が豊富に含まれているため、モロヘイヤの搾汁成分(エキス)を添加してなる食品や飲料等が多く上市されている。 Morohaya leaves and stems are rich in minerals such as potassium and calcium, β-carotene and vitamins, as well as mucus polysaccharides that lower blood cholesterol and neutral fat, such as glucose and arabinose. It is. As described above, since Morohaya contains abundant ingredients that are considered to be good for the body, many foods, beverages, and the like are added to the market.
その一方で、モロヘイヤは、高粘質成分としての酸性多糖類を多く含んでおり、独特の粘性特性(ぬめり)を持つことから、搾汁(固液分離)が困難であるばかりか、そのエキスは、粘性(ぬめり)を有しているがゆえに他の飲料原料等と均一に混合することが難しかったり、飲料自体にぬめり感が生じて飲み難くなったりするなどの問題を抱えていた。 On the other hand, moroheiya contains a lot of acidic polysaccharides as a high-viscosity component and has a unique viscosity characteristic (slimy). Has a problem that it is difficult to uniformly mix with other beverage ingredients and the like, and the beverage itself has a feeling of sliminess and is difficult to drink.
そこで従来、モロヘイヤ等のぬめりを有する野菜からエキスを得る際に、粘性(ぬめり)を低下させる方法が提案されている。
例えば特許文献1(特開平10−191922号公報)では、モロヘイヤを有機酸又は有機酸塩を添加した熱水で抽出することで、モロヘイヤエキス(モロヘイヤ抽出液)の粘度を低下させる技術が提案されている。
Therefore, conventionally, a method has been proposed for reducing the viscosity (slimming) when obtaining an extract from vegetables having sliminess such as moroheiya.
For example, Patent Document 1 (Japanese Patent Laid-Open No. 10-191922) proposes a technique for reducing the viscosity of a moroheiya extract (moroheia extract) by extracting moroheiya with hot water to which an organic acid or an organic acid salt is added. ing.
また、特許文献2(特開2000−228962号公報)は、ぬめりを有する野菜を搾汁して野菜搾汁液を製造するに際し、搾汁の前処理工程として少なくとも(a)野菜を冷凍後、解凍する工程、および/または、搾汁の後処理工程として少なくとも(b)野菜搾汁液に有機酸を添加する工程を含むことを特徴とする野菜搾汁液の製造方法を開示している。 Moreover, patent document 2 (Unexamined-Japanese-Patent No. 2000-228962) squeezes the vegetable which has a slime, and produces a vegetable juice, At least (a) As a pretreatment process of juice, it thaws | freezes the vegetable after freezing The manufacturing method of the vegetable juice characterized by including the process of adding an organic acid to at least (b) vegetable juice as a process to perform and / or a post-processing process of juice is disclosed.
本発明の課題は、モロヘイヤ等のぬめりを有する野菜から野菜エキス(搾汁成分)を得る際に、そのぬめり感を低減させることができる、新たな野菜エキスの製造方法を提案することにある。 The subject of this invention is providing the manufacturing method of the new vegetable extract which can reduce the slimy feeling, when obtaining a vegetable extract (squeezed component) from the vegetables which have sliminess, such as Morohaya.
かかる課題に鑑みて、本発明は、有機酸又は有機酸塩を含有する熱水でぬめり成分を含有する野菜をブランチングする工程と、ポリガラクツロナーゼを用いて該野菜を酵素処理する工程と、該野菜を搾汁する工程とを備えた野菜エキスの製造方法を提案する。 In view of such problems, the present invention includes a step of branching a vegetable containing a slime component with hot water containing an organic acid or an organic acid salt, and a step of enzyme-treating the vegetable using polygalacturonase. proposes a method for producing a vegetable extract and a step of squeezing the vegetables.
本発明の製法によれば、搾汁液の粘性を低下することができるため、搾汁(固液分離)が容易である。また、本発明により得られた野菜エキスは、ぬめり感が少なく粘性が低下しているため、他の飲料原料等と容易に均一に混合することができ、しかも飲料にぬめり感を生じさせることがなく、飲み易い野菜飲料を調製することができる。 According to the production method of the present invention, since the viscosity of the squeezed liquid can be reduced, squeezing (solid-liquid separation) is easy. In addition, the vegetable extract obtained by the present invention has little sliminess and low viscosity, so it can be easily and uniformly mixed with other beverage ingredients, etc. And easy-to-drink vegetable drinks can be prepared.
本欄では、本発明を実施するための形態の一例について説明するが、本発明が下記に説明する実施形態に限定されるものではない。 In this section, an example of an embodiment for carrying out the present invention will be described, but the present invention is not limited to the embodiment described below.
(本野菜エキス製法)
本実施形態にかかる野菜エキスの製造方法(「本野菜エキス製法」と称する。)は、野菜を洗浄し、有機酸又は有機酸塩を添加した熱水でブランチングし、その後、粉砕し、加水し、次いで酵素処理を行い、搾汁する工程を備えた製造方法である。
但し、かかる製造方法の前、途中、後に、公知の処理を追加することは任意に可能である。
(This vegetable extract manufacturing method)
A method for producing a vegetable extract according to the present embodiment (referred to as “the present vegetable extract production method”) is to wash a vegetable, blanch with hot water to which an organic acid or an organic acid salt is added, and then grind and add water. Then, it is a manufacturing method provided with the process of performing enzyme treatment and squeezing.
However, it is arbitrarily possible to add a known process before, during and after the manufacturing method.
(原料)
本野菜エキス製法で原料として使用できる野菜は、モロヘイヤ、オクラ、ツルムラサキなど、粘性成分(すなわち、ぬめり成分)を含有する野菜である。中でも、粘性低下作用の点では、モロヘイヤやオクラが特に好適である。
モロヘイヤの場合、原料となるのは葉又は茎であるが、特に生葉が好ましい。
(material)
Vegetables that can be used as a raw material in the present vegetable extract manufacturing method are vegetables containing viscous components (that is, slimy components) such as Morohaya, okra, and Tsurumurasaki. Of these, moroheiya and okra are particularly suitable in terms of the effect of reducing the viscosity.
In the case of Morohaya, the raw material is leaves or stems, but fresh leaves are particularly preferable.
(洗浄)
野菜の洗浄方法は、公知の洗浄方法を任意に採用可能であり、水、お湯、そのほかの洗浄液で洗浄すればよい。
この際、有機酸又は有機酸塩を添加した水又はお湯(「有機酸溶液」ともいう)で洗浄するのが好ましい。有機酸溶液で洗浄することにより、ブランチング前に野菜に有機酸又は有機酸塩に接触させ付着させることで、ブランチング工程において、より均一に有機酸又は有機酸塩による粘性低下の効果を高めることができる。また、洗浄中においても粘性物質は野菜から排出されるため、通常の場合、洗浄液も粘性が高い状態となるが、洗浄液に有機酸を添加することで、これを軽減する事ができ、次工程以降への粘性物質の持ち込みを減らすことができる。
この際、添加する有機酸又は有機酸塩としては、L−アスコルビン酸、クエン酸、リンゴ酸、酒石酸、又はフマル酸などの食品添加物として認可されている有機酸及びこれらの塩(例えばアスコルビン酸ナトリウム)を挙げることができる。中でも、粘性低下効果の点で、L-アスコルビン酸やクエン酸が好ましい。
洗浄方法を特に限定するものではなく、例えば洗浄槽内の洗浄液に有機酸を添加して溶解させ、この中に野菜を投入して洗浄すればよい。
有機酸又は有機酸塩の添加量は、洗浄液の0.01〜5%、特に0.05〜2%、中でも特に0.1〜1%とするのが好ましい。
(Washing)
As a vegetable washing method, a known washing method can be arbitrarily adopted, and it may be washed with water, hot water, or other washing liquid.
At this time, it is preferable to wash with water or hot water (also referred to as “organic acid solution”) to which an organic acid or an organic acid salt is added. By washing with an organic acid solution and bringing it into contact with the organic acid or organic acid salt on the vegetable before blanching, the effect of reducing the viscosity due to the organic acid or organic acid salt is more evenly increased in the blanching process. be able to. In addition, since viscous substances are discharged from vegetables even during washing, the washing liquid is usually in a highly viscous state, but this can be reduced by adding an organic acid to the washing liquid. It is possible to reduce the introduction of viscous substances to the subsequent.
At this time, as an organic acid or an organic acid salt to be added, organic acids approved as food additives such as L-ascorbic acid, citric acid, malic acid, tartaric acid, or fumaric acid and salts thereof (for example, ascorbic acid) Sodium). Among these, L-ascorbic acid and citric acid are preferable from the viewpoint of the viscosity reducing effect.
The cleaning method is not particularly limited, and for example, an organic acid may be added to and dissolved in the cleaning liquid in the cleaning tank, and the vegetables may be poured into the cleaning solution for cleaning.
The addition amount of the organic acid or organic acid salt is preferably 0.01 to 5%, particularly 0.05 to 2%, and particularly preferably 0.1 to 1% of the cleaning liquid.
(ブランチング)
洗浄後、有機酸又は有機酸塩を添加した熱水で野菜をブランチングすることが重要である。
ブランチングの本来の目的は、酵素失活により野菜に付着している微生物の殺菌、貯蔵中の品質の低下や変色の防止などにあるが、ブランチング溶液に有機酸又は有機酸塩を添加して処理することにより、得られる野菜エキスの粘性を効果的に低下させ、ぬめり感を無くすことができる。
(Branching)
After washing, it is important to blanch the vegetables with hot water to which an organic acid or organic acid salt has been added.
The original purpose of blanching is to sterilize microorganisms adhering to vegetables due to enzyme inactivation, to prevent deterioration of quality and discoloration during storage. However, organic acids or organic acid salts are added to blanching solutions. Treatment can effectively reduce the viscosity of the obtained vegetable extract and eliminate the slimy feeling.
添加する有機酸又は有機酸塩としては、L−アスコルビン酸、クエン酸、リンゴ酸、酒石酸、又はフマル酸などの食品添加物として認可されている有機酸及びこれらの塩(例えばアスコルビン酸ナトリウム)を挙げることができる。
中でも粘度低下効果の点で、アスコルビン酸およびクエン酸が好ましい。
Organic acids or organic acid salts to be added include organic acids approved as food additives such as L-ascorbic acid, citric acid, malic acid, tartaric acid, or fumaric acid, and salts thereof (for example, sodium ascorbate). Can be mentioned.
Of these, ascorbic acid and citric acid are preferred in terms of the effect of reducing the viscosity.
有機酸の添加量は、水又は熱水に対して0.01〜5質量%とするのが好ましい。この範囲であれば、粘性を低下させることができ、しかも酸味が強くなり過ぎることがなく、モロヘイヤ特有の風味を損なうことがない。
かかる観点から、有機酸の添加量は、水又は熱水に対して0.05〜2質量%するのが特に好ましく、中でも0.1〜0.5質量%とするのがさらに好ましい。
The addition amount of the organic acid is preferably 0.01 to 5% by mass with respect to water or hot water. If it is this range, a viscosity can be reduced, and also the acidity will not become too strong, and the flavor peculiar to Morohea will not be impaired.
From this viewpoint, the addition amount of the organic acid is particularly preferably 0.05 to 2% by mass with respect to water or hot water, and more preferably 0.1 to 0.5% by mass.
ブランチングの具体的方法としては、熱水に有機酸又は有機酸塩を添加してブランチング溶液を用意しておき、例えば野菜を網籠に入れてこの籠ごとブランチング溶液に浸漬させればよい。
この際、60〜100℃、特に65〜95℃の熱水で、品温が60〜100℃、特に65〜95℃に達するようにブランチングすればよく、ブランチング時間、すなわち浸漬時間は30秒〜7分、特に45秒〜5分程度とするのが好ましい。
As a specific method of blanching, a blanching solution is prepared by adding an organic acid or an organic acid salt to hot water. For example, if vegetables are put in a net koji and soaked in the blanching solution together with this koji Good.
At this time, it is only necessary to blanch with hot water of 60 to 100 ° C., particularly 65 to 95 ° C. so that the product temperature reaches 60 to 100 ° C., particularly 65 to 95 ° C. Second to 7 minutes, particularly about 45 seconds to 5 minutes are preferable.
(粉砕)
ブランチングした野菜は、必要に応じて、2軸エクストルーダー、スクリュープレス、グレーダー、ジューサー、ローラー式破砕機やミル等を用いて粉砕乃至破砕するのが好ましい。
この際、粉砕乃至破砕の程度は、5mm以下とするのが好ましい。
(Pulverization)
The blanched vegetables are preferably pulverized or crushed using a biaxial extruder, a screw press, a grader, a juicer, a roller crusher, a mill, or the like, if necessary.
At this time, the degree of pulverization or crushing is preferably 5 mm or less.
(加水)
粉砕後、水を加えるのが好ましい。
野菜を粉砕乃至破砕した状態では攪拌及び搾汁が困難であるため、攪拌によって均一に酵素処理を行わせるために、酵素処理の前段階で加水するのが好ましい。
(Hydro)
It is preferable to add water after grinding.
Since it is difficult to stir and squeeze the vegetables in a pulverized or crushed state, it is preferable to add water before the enzyme treatment in order to perform the enzyme treatment uniformly by stirring.
加える水は、ミネラル水、天然水、イオン交換水、精製水、脱気水、水道水等の水を挙げることができる。
この際の加水量は、少な過ぎると酵素が全体に作用しなくなり、逆に多すぎると同様に野菜への酵素の作用(接触)が少なくなると共に、その後の濃縮工程等での生産効率も悪くなるため、かかる観点から、野菜(原料)に対して20〜500質量%、特に50〜200質量%、中でも100〜150質量%とするのが好ましい。
Examples of water to be added include mineral water, natural water, ion exchange water, purified water, deaerated water, tap water, and the like.
If the amount of water added is too small, the enzyme does not act on the whole. Conversely, if the amount of water is too large, the action (contact) of the enzyme on the vegetable is reduced, and the production efficiency in the subsequent concentration step is poor. Therefore, from this viewpoint, it is preferably 20 to 500% by mass, particularly 50 to 200% by mass, and more preferably 100 to 150% by mass with respect to the vegetable (raw material).
(酵素処理)
次に、野菜を酵素処理することが好ましい。
有機酸溶液によるブランチングと酵素処理とを組み合わせて実施することにより、野菜エキスの粘性低下作用を相乗的に高めることができる。
(Enzyme treatment)
Next, it is preferable to carry out the enzyme treatment of vegetables.
By carrying out a combination of blanching with an organic acid solution and enzyme treatment, it is possible to synergistically increase the viscosity-reducing action of the vegetable extract.
粘性低下作用を示す酵素としては、ペクチナーゼ、α-アミラーゼ、グルコアミラーゼ、ポリガラクツロナーゼ、βグルカナーゼ、プロテアーゼ、アミノペプチターゼなどを挙げることができ、これらを単独又は二種類以上を組み合わせて用いることができる。
中でも、粘性低下作用の点で、α-アミラーゼ、グルコアミラーゼ、ポリガラクツロナーゼ、βグルカナーゼ、プロテアーゼが好ましく、その中でもグルコアミラーゼ、ポリガラクツロナーゼ、βグルカナーゼがより好ましく、その中でもポリガラクツロナーゼが特に好ましい。
さらにポリガラクツロナーゼと、前述の酵素、例えばペクチナーゼ、α-アミラーゼ、グルコアミラーゼ、βグルカナーゼ、プロテアーゼ及びアミノペプチターゼのうちの一種又は二種以上とを組み合わせて用いるのが好ましく、その中でも、グルコアミラーゼ及びβグルカナーゼの一種又は二種以上と組み合わせて用いるのが好ましい。
Examples of the enzyme having a viscosity-reducing action include pectinase, α-amylase, glucoamylase, polygalacturonase, β-glucanase, protease, aminopeptidase, etc., and these may be used alone or in combination of two or more. Can do.
Of these, α-amylase, glucoamylase, polygalacturonase, β-glucanase, and protease are preferable from the viewpoint of viscosity-reducing action, and glucoamylase, polygalacturonase, and β-glucanase are more preferable, and polygalacturonase is particularly preferable. Is particularly preferred.
Furthermore, it is preferable to use polygalacturonase in combination with one or more of the above-mentioned enzymes such as pectinase, α-amylase, glucoamylase, β-glucanase, protease and aminopeptidase. It is preferably used in combination with one or more of amylase and β-glucanase.
酵素の量は、野菜(原料)の0.01〜2質量%、特に0.05〜1質量%、中でも0.1〜0.5質量%とするのが好ましい。 The amount of the enzyme is preferably 0.01 to 2% by mass, particularly 0.05 to 1% by mass, particularly 0.1 to 0.5% by mass of the vegetable (raw material).
酵素処理の方法は、野菜に酵素を加えて酵素反応させることができれば特に具体的方法を特定するものではない。例えば、野菜を粉砕し、得られた野菜粉砕物に加水した状態で酵素を加えて酵素反応させるようにすればよい。
この際、野菜粉砕物に加水した状態の温度は、酵素反応を促進させるため、30〜70℃、特に35〜60℃、中でも特に40〜55℃に調整するのが好ましく、必要に応じて攪拌するのがよい。
なお、添加した酵素は、その後の処理工程、例えば殺菌工程等で加熱され失活されることになる。
The enzyme treatment method is not particularly specified as long as an enzyme can be added to the vegetables to cause an enzyme reaction. For example, an enzyme reaction may be performed by pulverizing vegetables and adding an enzyme in a state of being added to the obtained pulverized vegetable product.
At this time, the temperature of the hydrated vegetable product is preferably adjusted to 30 to 70 ° C., particularly 35 to 60 ° C., particularly 40 to 55 ° C. in order to promote the enzyme reaction, and stirred as necessary. It is good to do.
In addition, the added enzyme is heated and inactivated in a subsequent processing step such as a sterilization step.
(搾汁)
野菜の搾汁は、例えばデカンター、フィルタープレス、スクリュープレス、二軸回転型エクストルーダー、ジューサー等、現在公知の搾汁機を用いて搾汁すればよい。
また、野菜の種類によっては、クラッシャーによる破砕及びデカンターによる搾汁を行って粗搾汁液を得、この粗搾汁液を濾過するようにしてもよい。例えば破砕乃至磨砕した後、ネルろ過により搾汁した後、遠心分離して搾汁液を得ることもできる。
(Squeezed juice)
The vegetable juice may be squeezed using a currently known juicer such as a decanter, a filter press, a screw press, a twin-screw extruder, a juicer, or the like.
Moreover, depending on the kind of vegetable, crushing with a crusher and squeezing with a decanter may be performed to obtain a crude juice, and the crude juice may be filtered. For example, after crushing or grinding, the juice may be squeezed by flannel filtration and then centrifuged to obtain a juice.
(後処理)
このようにして得られた野菜エキスは、即座に飲料や食品の原料として使用することもできるが、保管するようにしてもよい。また、必要に応じて成分調整、濃縮、乾燥、殺菌などの処理をしてもよい。
(Post-processing)
The vegetable extract thus obtained can be used immediately as a raw material for beverages and foods, but may be stored. Moreover, you may process components adjustment, concentration, drying, disinfection, etc. as needed.
殺菌方法は、通常の飲料と同様に行えばよい。例えば金属缶のように容器に充填後、加熱殺菌できる場合にあっては食品衛生法に定められた殺菌条件で殺菌を行えばよい。また、PETボトル、紙容器のようにレトルト殺菌できないものについては、例えばプレート式熱交換器などで高温殺菌後冷却して容器に充填するなどすればよい。 What is necessary is just to perform the disinfection method similarly to a normal drink. For example, in the case where heat sterilization can be performed after filling a container like a metal can, sterilization may be performed under the sterilization conditions defined in the Food Sanitation Law. Moreover, what cannot be retort sterilized like a PET bottle and a paper container should just cool after high temperature sterilization with a plate-type heat exchanger etc., for example, and may fill with a container.
必要に応じてpH調整剤を添加してもよい。pH調整剤としては、例えばクエン酸、リンゴ酸、酒石酸、酢酸、乳酸、及びグルコン酸等の有機酸、レモン、アセロラ及びカムカム等の果汁を挙げることができる。
調整するpH範囲は特に限定するものではないが、一般的には野菜飲料の保存安定性及び飲み易さの点からpH3〜6とするのが好ましい。
You may add a pH adjuster as needed. Examples of the pH adjuster include organic acids such as citric acid, malic acid, tartaric acid, acetic acid, lactic acid, and gluconic acid, and fruit juices such as lemon, acerola, and camcam.
The pH range to be adjusted is not particularly limited, but generally it is preferably 3 to 6 from the viewpoint of storage stability and ease of drinking of vegetable drinks.
(用途)
本発明で添加する有機酸は食品添加物として認可されており、人体に安全なものである。よって、得られた野菜エキスは、野菜飲料や果汁飲料の飲料原料として使用することができるほか、各種食品に添加することもできる。また、健康を増進するサプリメント、栄養補助食品の原料として使用することもできる。
(Use)
The organic acid added in the present invention is approved as a food additive and is safe for the human body. Therefore, the obtained vegetable extract can be used as a beverage material for vegetable beverages and fruit juice beverages, and can also be added to various foods. It can also be used as a raw material for supplements and nutritional supplements that promote health.
(用語の説明)
本発明における「野菜エキス」とは、野菜の搾汁成分からなる物質の意味であり、その形態は液状、ピューレ状、粉末状、固形状など任意である。
本明細書において「X〜Y」(X,Yは任意の数字)と表現する場合、特にことわらない限り「X以上Y以下」の意と共に、「好ましくはXより大きい」及び「好ましくはYより小さい」の意を包含する。
また、「X以上」或いは「Y以下」(X,Yは任意の数字)と表現する場合、特にことわらない限り、「好ましくはXより大きい」或いは「好ましくはYより小さい」の意を包含する。
(Explanation of terms)
The “vegetable extract” in the present invention means a substance composed of vegetable juice components, and the form thereof is arbitrary such as liquid, puree, powder, and solid.
In the present specification, when expressed as “X to Y” (X and Y are arbitrary numbers), unless otherwise specified, “preferably greater than X” and “preferably Y” together with the meaning of “X to Y”. It means “smaller”.
Further, when expressed as “X or more” or “Y or less” (X and Y are arbitrary numbers), it means “preferably larger than X” or “preferably smaller than Y” unless otherwise specified. To do.
次に、試験例に基づいて本発明について更に説明するが、本発明が以下に示す実施例に限定されるものではない。 Next, although this invention is further demonstrated based on a test example, this invention is not limited to the Example shown below.
<試験1:有機酸溶液によるブランチング効果検討>
有機酸溶液を用いたブランチングによる粘性低下効果を検討した。
<Test 1: Study of blanching effect with organic acid solution>
The viscosity reduction effect by blanching using organic acid solution was investigated.
モロヘイヤ生葉300gを、表1に示したブランチング溶液(100℃)で3分間ブランチングし、水切りをした後、ローラー式破砕機で5mm片程度に粉砕した。粉砕したモロヘイヤに原料量と同量の水を加え、20秒間攪拌した後、遠心分離機にて3000rpm10分間の処理を行い、60メッシュ篩にて固液分離し、得られたモロヘイヤエキスの粘度測定を行った。
粘度測定は、約25℃にてB型粘度計を用いて測定した。
300 g of raw moroheiya leaves were blanched for 3 minutes with the blanching solution (100 ° C.) shown in Table 1, drained, and then crushed to about 5 mm pieces with a roller crusher. Add the same amount of water to the crushed moroheiya and stir for 20 seconds, then process at 3000 rpm for 10 minutes with a centrifuge, solid-liquid separate with a 60 mesh sieve, and measure the viscosity of the obtained moroheia extract Went.
Viscosity was measured at about 25 ° C. using a B-type viscometer.
(結 果)
水によるブランチングよりも、アスコルビン酸又はクエン酸を加えた有機酸溶液でブランチングした方が粘度が低下することが確認できた。
このような有機酸の作用は、酸による加水分解によるものと考えられるため、アスコルビン酸やクエン酸以外の有機酸についても同様の効果があるものと推測される。
上記試験及びその他の試験結果からすると、ブランチング溶液としての有機酸濃度は、0.01〜5%の範囲であればよく、好ましくは0.1〜0.5%であるのが好ましいものと考えることができる。
(Result)
It was confirmed that the viscosity decreased when blanched with an organic acid solution to which ascorbic acid or citric acid was added, rather than with water.
Since the action of such an organic acid is considered to be due to hydrolysis by an acid, it is presumed that the organic acid other than ascorbic acid and citric acid has the same effect.
From the above test and other test results, the organic acid concentration as the blanching solution may be in the range of 0.01 to 5%, preferably 0.1 to 0.5%. Can think.
<試験2:酵素処理による粘性低下効果の検討>
酵素処理による粘性低下効果を検討した。
<Test 2: Examination of viscosity reduction effect by enzyme treatment>
The effect of viscosity reduction by enzyme treatment was examined.
モロヘイヤ生葉を、100℃の熱水で3分間ブランチングし、粗粉砕した後、冷凍保存してモロヘイヤピューレを調製した。
このモロヘイヤピューレ400gをローラー式破砕機にて5mm片程度に粉砕し、湯煎にて80℃に加温後、原料(モロヘイヤピューレ)と同量の水を加え、ウォーターバスにて品温が42〜45℃となるように調整した。次に、原料(モロヘイヤピューレ)量に対し0.2質量%の酵素(表2参照)を加え、50℃のウォーターバスにて15分間攪拌して酵素処理した後、遠心分離機にて3000rpm10分間の処理を行い、60メッシュ篩にて固液分離し、得られたモロヘイヤエキスの粘度測定を行った。
Morohaya pure leaves were blanched with hot water at 100 ° C. for 3 minutes, coarsely pulverized, and stored frozen to prepare Morohaya puree.
400 g of this moroheiya puree is pulverized into about 5 mm pieces with a roller crusher, heated to 80 ° C. with a hot water bath, and then added with the same amount of water as the raw material (moloheia puree). The temperature was adjusted to 45 ° C. Next, 0.2% by mass of the enzyme (see Table 2) is added to the amount of the raw material (Morohaya puree), and the mixture is stirred for 15 minutes in a 50 ° C. water bath and treated with an enzyme, and then centrifuged at 3000 rpm for 10 minutes. The solid-liquid separation was performed with a 60 mesh sieve, and the viscosity of the obtained moroheiya extract was measured.
粘度測定は、約25℃にてB型粘度計を用いて測定した。
表2の搾汁液回収率については、原料(モロヘイヤピューレ)量400g+加水量400gの合計800gに対する回収率(%)として算出した。
また、酵素処理を行わない以外は上記と同様の方法でモロヘイヤエキスを製造し、これをコントロールとし、コントロールの粘度に対する比率を表2に示した。
Viscosity was measured at about 25 ° C. using a B-type viscometer.
About the juice recovery rate of Table 2, it calculated as a recovery rate (%) with respect to a total of 800 g of 400 g of raw material (Morohaya puree) +400 g of water.
Further, a morohea extract was produced in the same manner as described above except that the enzyme treatment was not performed, and this was used as a control. The ratio of the control to the viscosity is shown in Table 2.
(結 果)
各種酵素を試したところ、ペクチナーゼ、プロテアーゼ、α-アミラーゼ、グルコアミラーゼ、ポリガラクツロナーゼ、アミノペプチターゼ、β-グルカナーゼなどの酵素に粘性低下効果が認められた。
(Result)
When various enzymes were tested, viscosity-reducing effects were observed for enzymes such as pectinase, protease, α-amylase, glucoamylase, polygalacturonase, aminopeptidase, and β-glucanase.
<試験3:有機酸ブランチングと酵素処理の組合せによる粘性低下効果の検討>
有機酸ブランチングと酵素処理とを組合せた場合の粘性低下効果を検討した。
<Test 3: Examination of the effect of reducing viscosity by the combination of organic acid blanching and enzyme treatment>
The effect of viscosity reduction when combining organic acid blanching and enzyme treatment was investigated.
モロヘイヤ生葉300gを、3000g量の水(通常ブランチング、100℃)もしくはクエン酸0.05%溶液(100℃、pH3.1)で3分間ブランチングし、水切り後にローラー式破砕機で5mm片程度に粉砕した。
次に、原料(モロヘイヤ生葉)と同量の300gの水を加え、50℃のウォーターバスにて品温が42〜45℃になるまで加温した後、6種類の酵素(ペクチナーゼ、αアミラーゼ、グルコアミラーゼ、ポリガラクツロナーゼ、アミノペプチターゼ、βグルカナーゼ)全てを原料量に対して0.2質量%ずつ添加し、50℃ウォーターバスにて15分間攪拌して酵素処理した後、遠心分離機にて3000rpm10分間の処理を行い、60メッシュ篩にて固液分離し、得られたモロヘイヤエキスの粘度測定を行った。
粘度測定は、約25℃にてB型粘度計を用いて測定した。
300 g of raw moroheiya leaves are blanched for 3 minutes with 3000 g of water (normally blanched, 100 ° C.) or 0.05% citric acid solution (100 ° C., pH 3.1). After draining, about 5 mm pieces using a roller crusher To grind.
Next, after adding 300 g of water in the same amount as the raw material (Moloheiya fresh leaves) and heating in a 50 ° C. water bath until the product temperature reaches 42 to 45 ° C., six types of enzymes (pectinase, α-amylase, (Glucoamylase, polygalacturonase, aminopeptidase, β-glucanase) are all added in an amount of 0.2% by mass with respect to the amount of the raw material, and the mixture is stirred for 15 minutes in a 50 ° C. water bath for enzyme treatment, and then centrifuged. Was processed at 3000 rpm for 10 minutes, and solid-liquid separation was performed with a 60 mesh sieve, and the viscosity of the obtained morohea extract was measured.
Viscosity was measured at about 25 ° C. using a B-type viscometer.
(結 果)
通常ブランチングと酵素処理とを組み合わせて処理したものに比較して、クエン酸溶液ブランチングと酵素処理とを組み合わせて処理したものは、粘度が大きく減少した。
これより、有機酸溶液ブランチングと酵素処理とを組み合わせると相乗的に粘度低下効果が高まるものと考えられる。
(Result)
Compared to the case where the blanching and the enzyme treatment were combined in combination, the case where the citric acid solution blanching and the enzyme treatment were combined was greatly reduced in viscosity.
From this, it is considered that when the organic acid solution blanching and the enzyme treatment are combined, the effect of reducing the viscosity is synergistically increased.
<試験4:有機酸溶液との組合せにおいて特に粘性を低減させる酵素類の検討>
試験3で使用した6種類の酵素のうち、特に粘性を低減させている種類を検討する試験を行った。
<Test 4: Examination of enzymes that reduce viscosity especially in combination with organic acid solution>
Among the six types of enzymes used in Test 3, a test was conducted to examine the types that particularly reduced the viscosity.
モロヘイヤ生葉300gを、3000g量の水(通常ブランチング、100℃)もしくはクエン酸0.05%溶液(100℃、pH3.1)で3分間ブランチングし、水切り後にローラー式破砕機で5mm片程度に粉砕した。
次に、原料(モロヘイヤ生葉)と同量の300gの水を加え、50℃のウォーターバスにて品温が42〜45℃になるまで加温した後、6種類の酵素(ペクチナーゼ、αアミラーゼ、グルコアミラーゼ、ポリガラクツロナーゼ、アミノペプチターゼ、βグルカナーゼ)のうち、試験(1)ではペクチナーゼ以外の5種類、試験(2)ではペクチナーゼ、アミノペプチターゼ以外の4種類の酵素を用いて酵素処理を行った。この際、酵素量は処理(1)〜(7)は原料に対して各0.2質量%、処理(8)では各0.1質量%とした。そして、50℃ウォーターバスにて15分間攪拌して酵素処理した後、遠心分離機で3000rpm10分間の処理を行い、60メッシュ篩にて固液分離し、得られたモロヘイヤエキスの粘度測定を行った。
粘度測定は、約25℃にてB型粘度計を用いて測定した。
300 g of raw moroheiya leaves are blanched for 3 minutes with 3000 g of water (normally blanched, 100 ° C.) or 0.05% citric acid solution (100 ° C., pH 3.1). After draining, about 5 mm pieces using a roller crusher To grind.
Next, after adding 300 g of water in the same amount as the raw material (Moloheiya fresh leaves) and heating in a 50 ° C. water bath until the product temperature reaches 42 to 45 ° C., six types of enzymes (pectinase, α-amylase, (Glucoamylase, polygalacturonase, aminopeptidase, β-glucanase) In test (1), five types of enzymes other than pectinase and in test (2), four types of enzymes other than pectinase and aminopeptidase are used for enzyme treatment Went. At this time, the amount of enzyme was set to 0.2% by mass for the raw materials for the treatments (1) to (7) and 0.1% by mass for the treatment (8). Then, after stirring for 15 minutes in a 50 ° C. water bath and performing enzyme treatment, the mixture was subjected to treatment at 3000 rpm for 10 minutes with a centrifugal separator, solid-liquid separation was performed with a 60 mesh sieve, and the viscosity of the resulting moroheiya extract was measured. .
Viscosity was measured at about 25 ° C. using a B-type viscometer.
(水切具合の評価)
水切具合の評価は、次の基準で評価した。後述する試験でも同様である
○:篩を通した際に、搾汁液が水滴状になって落下した場合に○と評価した。
△:篩を通した際に、搾汁液が糸を引く状態(5cm以上の糸引き状態)で落下した場合に△と評価した。
×:篩の上にゲル状に滞留し、篩をスムーズに通過していかない状態の場合に×と評価した。
(Evaluation of drainage)
The drainage condition was evaluated according to the following criteria. The same applies to the test described later. ○: When the squeezed liquid falls in the form of water droplets when passing through a sieve, it was evaluated as ○.
(Triangle | delta): When passing through the sieve, it evaluated as (triangle | delta) when the squeezed liquid fell in the state (pulling state of 5 cm or more) which pulled the thread | yarn.
X: It was evaluated as x when the gel stayed on the sieve and did not pass through the sieve smoothly.
(結 果)
処理(2)〜(4)では、試験3で使用した6種類の酵素のうち、ペクチナーゼ、アミノペプチターゼ、αアミラーゼを除外して添加したが、6種類全部を加えたと同様に搾汁液の粘度を低下させることができた。
処理(5)及び処理(6)では、6種類の酵素のうち、グルコアミラーゼ、βグルカナーゼを除いて添加したが、搾汁液の粘度低下効果は弱まり、搾汁液も糸を引く状態となった。
処理(7)では、ポリガラクツロナーゼを除いた結果、搾汁液の粘度低下効果は顕著に弱くなった。
この結果より、6種類の酵素の中でも、特にポリガラクツロナーゼ処理は粘度低減に効果が高いことが分かった。さらにβグルカナーゼ及びグルコアミラーゼを加えた3種類での酵素処理は効果が特に高いことが分かった。
(Result)
In the treatments (2) to (4), pectinase, aminopeptidase and α-amylase were excluded from the six types of enzymes used in Test 3, but the viscosity of the juice was the same as when all six types were added. Could be reduced.
In treatment (5) and treatment (6), glucoamylase and β-glucanase were added out of the six types of enzymes, but the effect of lowering the viscosity of the juice was weakened, and the juice also pulled the yarn.
In the treatment (7), as a result of removing polygalacturonase, the viscosity reduction effect of the juice was significantly weakened.
From these results, it was found that among the six types of enzymes, the treatment with polygalacturonase was particularly effective in reducing the viscosity. Furthermore, it was found that the enzyme treatment with three kinds added with β-glucanase and glucoamylase was particularly effective.
処理(8)では、ポリガラクツロナーゼ、βグルカナーゼ、グルコアミラーゼの3種類の酵素量を各0.1質量%に少なくして処理を行った。その結果、各0.1質量%でも、粘度低減効果は確認された。しかしながら、0.2質量%の場合と比較すると、やや粘性も高く(処理(4))、糸を引く状態であった。
なお、搾汁液のBrix(以下「Bx」と略す)はコントロールと比較すると処理(2)〜(8)のいずれも高くなった。
In the treatment (8), the treatment was performed by reducing the amounts of three kinds of enzymes, polygalacturonase, β-glucanase, and glucoamylase, to 0.1% by mass. As a result, even at 0.1% by mass, the viscosity reducing effect was confirmed. However, compared with the case of 0.2% by mass, the viscosity was slightly higher (Process (4)) and the yarn was pulled.
In addition, all of processing (2)-(8) became high compared with Brix (henceforth "Bx") of squeezed liquid.
<試験5:添加する酵素量(特にβグルカナーゼ、グルコアミラーゼ)の検討>
添加する酵素量(特にβグルカナーゼ、グルコアミラーゼ)について検討した。
<Test 5: Examination of the amount of enzyme to be added (particularly β-glucanase, glucoamylase)>
The amount of enzyme to be added (particularly β-glucanase and glucoamylase) was examined.
モロヘイヤ生葉300gを、3000g量の水(通常ブランチング、100℃)もしくはクエン酸0.05%溶液(100℃、pH3.1)で3分間ブランチングし、水切り後にローラー式破砕機で5mm片程度に粉砕した。
次に、原料(モロヘイヤ生葉)と同量の300gの水を加え、50℃のウォーターバスにて品温が42〜45℃になるまで加温した後、3種類の酵素(ポリガラクツロナーゼ、βグルカナーゼ、グルコアミラーゼ)を用いて、処理(2)では各0.2%(対原料)、処理(3)(4)では下記表の通りの濃度で投入し、50℃ウォーターバスにて15分間攪拌して酵素処理した後、遠心分離機で3000rpm10分間の処理を行い、60メッシュ篩にて固液分離し、得られたモロヘイヤエキスの粘度測定を行った。
粘度測定は、約25℃にてB型粘度計を用いて測定した。
300 g of raw moroheiya leaves are blanched for 3 minutes with 3000 g of water (normally blanched, 100 ° C.) or 0.05% citric acid solution (100 ° C., pH 3.1). After draining, about 5 mm pieces using a roller crusher To grind.
Next, 300 g of water in the same amount as the raw material (Moloheiya fresh leaves) was added and heated in a 50 ° C. water bath until the product temperature reached 42 to 45 ° C., then three types of enzymes (polygalacturonase, β-glucanase and glucoamylase) were added in the treatment (2) at 0.2% (vs. raw material), and in the treatments (3) and (4) at the concentrations shown in the table below. The mixture was stirred for minutes and subjected to an enzyme treatment, followed by treatment at 3000 rpm for 10 minutes with a centrifuge, solid-liquid separation with a 60 mesh sieve, and the viscosity of the obtained moroheiya extract was measured.
Viscosity was measured at about 25 ° C. using a B-type viscometer.
(結 果)
ポリガラクツロナーゼについては、試験4で0.1%にした時にやや粘度が高くなる傾向がみられ、0.2%以上であれば十分な粘度低下効果が認められた。これまでの試験結果からすると、原料の0.01〜2質量%の範囲で効果を示すと考えられる。
ポリガラクツロナーゼと併用するグルコアミラーゼやβグルカナーゼについては、原料の0.05〜0.2質量%で効果を示し、粘度を低下させた。これまでの試験結果からすると、原料の0.01〜2質量%の範囲で効果を示すと考えられる。
(Result)
With respect to polygalacturonase, a tendency that the viscosity was slightly increased when 0.1% was obtained in Test 4 was observed, and if it was 0.2% or more, a sufficient viscosity reducing effect was observed. From the test results so far, it is considered that the effect is exhibited in the range of 0.01 to 2% by mass of the raw material.
About glucoamylase and (beta) glucanase used together with polygalacturonase, the effect was shown by 0.05-0.2 mass% of the raw material, and the viscosity was reduced. From the test results so far, it is considered that the effect is exhibited in the range of 0.01 to 2% by mass of the raw material.
追加試験として(5)(6)を行った結果、処理(5)では、加水量を等量加水ではなく、半量加水にしたが、やや粘度は高くなるものの粘度低下効果は認められた。すなわち、上記の試験では、ブランチング後に破砕して加水する水の量を対原料量で等量とすることを基本としたが、半量加水でも粘度を低下させることができることが確かめられた。
また、処理(6)では、ゆで(ブランチング)時間を5分間としたが、粘度を低下させることができることが確かめられた。
As a result of performing (5) and (6) as an additional test, in the treatment (5), the amount of water was not equal but not half, but half the amount was added. That is, in the above test, the amount of water to be crushed and watered after blanching was basically equal to the amount of raw material, but it was confirmed that the viscosity could be reduced even by half-water addition.
In the treatment (6), the boiled (branching) time was 5 minutes, but it was confirmed that the viscosity could be lowered.
<試験6:モロヘイヤエキスの製法比較試験>
本発明による製法(実施例)と、従来公知の製法(比較例)とを比較検討した。
<Test 6: Production comparison test of Morohaya extract>
The production method (Example) according to the present invention was compared with a conventionally known production method (Comparative Example).
(比較例1:コントロール)
モロヘイヤ生葉300gを、3000g量の熱水(100℃)で3分間ブランチングし(品温約90℃)、水切り後にローラー式破砕機で5mm片程度に粉砕した。
次に、原料(モロヘイヤ生葉)と同量の300gの水を加え、50℃のウォーターバスにて15分間攪拌して加温した後、遠心分離機にて3000rpm10分間の処理を行い、60メッシュ篩にて濾過し、得られたモロヘイヤエキスの粘度測定を行った。
粘度測定は、約25℃にてB型粘度計を用いて測定した。
(Comparative Example 1: Control)
300 g of moroheiya fresh leaves were blanched for 3 minutes with an amount of hot water (100 ° C.) of 3000 g (product temperature: about 90 ° C.), and after draining, it was crushed to about 5 mm pieces with a roller crusher.
Next, after adding 300 g of water in the same amount as the raw material (Morohaya fresh leaves), stirring and heating in a 50 ° C. water bath for 15 minutes, a treatment at 3000 rpm for 10 minutes was performed in a centrifuge, and a 60 mesh sieve was obtained. The viscosity of the obtained moroheiya extract was measured.
Viscosity was measured at about 25 ° C. using a B-type viscometer.
(比較例2)
モロヘイヤ生葉300gを、3000g量の熱水(100℃)で3分間ブランチングし(品温約90℃)、水切り後にローラー式破砕機で5mm片程度に破砕した。
次に、原料(モロヘイヤ生葉)と同量の300gの水を加え、L−アスコルビン酸2.1g(加水量に対して0.7質量%)を加えて95℃に加温して20分間撹拌した後、遠心分離機にて3000rpm10分間の処理を行い、60メッシュ篩にて固液分離し、得られたモロヘイヤエキスの粘度測定を行った。
粘度測定は、約25℃にてB型粘度計を用いて測定した。
(Comparative Example 2)
300 g of raw moroheiya leaves were blanched for 3 minutes with an amount of hot water (100 ° C.) of 3000 g (product temperature: about 90 ° C.) and, after draining, crushed into about 5 mm pieces with a roller crusher.
Next, 300 g of water in the same amount as the raw material (Morohaya leaves) is added, 2.1 g of L-ascorbic acid (0.7% by mass with respect to the water content) is added, and the mixture is heated to 95 ° C. and stirred for 20 minutes. After that, a treatment at 3000 rpm for 10 minutes was performed with a centrifuge, solid-liquid separation was performed with a 60 mesh sieve, and the viscosity of the obtained moroheiya extract was measured.
Viscosity was measured at about 25 ° C. using a B-type viscometer.
(比較例3)
モロヘイヤ生葉300gを、3000g量の熱水(100℃)で3分間ブランチングし(品温約90℃)、水切り後に−40℃にて冷凍した。一晩経過後、自然解凍し、ローラー式破砕機で5mm片程度に破砕し、原料(モロヘイヤ生葉)と同量の300gの水を加え、遠心分離機にて3000rpm10分間の遠心分離を行い、L−アスコルビン酸1.5g(液に対して0.5質量%)を加えて90℃に加温して5分間撹拌した後、遠心分離機にて3000rpm10分間の処理を行い、60メッシュ篩にて固液分離し、得られたモロヘイヤエキスの粘度測定を行った。
粘度測定は、約25℃にてB型粘度計を用いて測定した。
(Comparative Example 3)
300 g of raw moroheiya leaves were blanched for 3 minutes with an amount of hot water (100 ° C.) of 3000 g (product temperature: about 90 ° C.), and frozen at −40 ° C. after draining. After one night, thaw naturally, crush to about 5 mm with a roller-type crusher, add 300 g of water in the same amount as the raw material (Moloheiya fresh leaves), centrifuge at 3000 rpm for 10 minutes in a centrifuge, -Ascorbic acid 1.5g (0.5% by mass with respect to the liquid) was added, heated to 90 ° C and stirred for 5 minutes, then treated at 3000 rpm for 10 minutes with a centrifuge, and then with a 60 mesh sieve. Solid-liquid separation was performed, and the viscosity of the obtained moroheiya extract was measured.
Viscosity was measured at about 25 ° C. using a B-type viscometer.
(実施例1)
モロヘイヤ生葉300gを、3000g量のクエン酸0.05%溶液(100℃)で3分間ブランチングし(品温約90℃)、水切り後にローラー式破砕機で5mm片程度に粉砕した。
次に、原料(モロヘイヤ生葉)と同量の300gの水を加え、50℃のウォーターバスにて品温が42〜45℃になるまで加温した後、酵素(ポリガラクツロナーゼ:0.2質量%、βグルカナーゼ:0.1質量%、グルコアミラーゼ:0.1質量%を含む)を添加し、50℃ウォーターバスにて15分間攪拌して酵素処理した後、遠心分離機にて3000rpm10分間の処理を行い、60メッシュ篩にて固液分離し、得られたモロヘイヤエキスの粘度測定を行った。
なお、各酵素の量(質量%)は原料(モロヘイヤ生葉)に対する質量割合である。
粘度測定は、約25℃にてB型粘度計を用いて測定した。
Example 1
300 g of raw moroheiya leaves were blanched with a 3000 g amount of a 0.05% citric acid solution (100 ° C.) for 3 minutes (product temperature: about 90 ° C.), drained, and crushed to about 5 mm pieces with a roller crusher.
Next, after adding 300 g of water in the same amount as the raw material (Moloheiya fresh leaves) and heating in a 50 ° C water bath until the product temperature reaches 42 to 45 ° C, the enzyme (polygalacturonase: 0.2) (Mass%, β-glucanase: 0.1% by mass, glucoamylase: 0.1% by mass included), stirred for 15 minutes in a 50 ° C. water bath and treated with an enzyme, and then centrifuged at 3000 rpm for 10 minutes. The solid-liquid separation was performed with a 60 mesh sieve, and the viscosity of the obtained moroheiya extract was measured.
In addition, the quantity (mass%) of each enzyme is a mass ratio with respect to a raw material (Moloheiya fresh leaf).
Viscosity was measured at about 25 ° C. using a B-type viscometer.
搾汁率の回収率(歩留り)は、実施例が最も高く、しかも粘度低下の効果も実施例が最も高い結果となった。
搾汁液のBxは、コントロール(比較例1)と比較して実施例1は高かった。
搾汁液のpHは、コントロールと比較して実施例1は低かった。
搾汁液中のカルシウム(Ca)量は、コントロールと比較して実施例1は大きく増加した。
The recovery rate (yield) of the squeezing rate was the highest in Examples, and the effect of reducing the viscosity was the highest in Examples.
The Bx of the juice was higher in Example 1 than in the control (Comparative Example 1).
The pH of the juice was lower in Example 1 than in the control.
The amount of calcium (Ca) in the squeezed liquid was greatly increased in Example 1 compared to the control.
<試験7:飲料適正に関する官能評価>
市販の野菜・果実ミックスジュースに、試験6の比較例1、2、実施例1で得られたモロヘイヤエキス(Bx2調整品)を2%添加し、8名による官能評価を実施した。
<Test 7: Sensory evaluation on beverage adequacy>
To the commercially available vegetable / fruit mixed juice, 2% of the moroheiya extract (Bx2 preparation) obtained in Comparative Examples 1 and 2 and Test 1 of Test 6 was added, and sensory evaluation by 8 persons was performed.
コントロールに対する評価として次の5段階で評価し、表7には8名の平均値を示した。
(評価基準)
−2:かなり弱い
−1:弱い
0:普通
+1:強い
+2:かなり強い
As the evaluation for the control, the evaluation was carried out in the following five stages, and Table 7 shows the average value of 8 persons.
(Evaluation criteria)
-2: Pretty weak -1: Weak 0: Normal +1: Strong +2: Pretty strong
実施例1の製法で得られたモロヘイヤエキスを使用すると、野菜飲料の「飲み易さ」「スッキリ感」が高くなる傾向が認められた。また、「青臭さ」も若干低くなる傾向が認められた。 When the morohea extract obtained by the manufacturing method of Example 1 was used, the tendency for the "easiness to drink" and "a refreshing feeling" of a vegetable drink to be increased was recognized. In addition, the “blue odor” tended to be slightly lower.
<モロヘイヤ以外の野菜での効果検討>
次に、モロヘイヤ以外の粘性含有野菜への転用を検討した。
<Examination of effects with vegetables other than Moroheiya>
Next, diversion to viscous vegetables other than moroheiya was examined.
(コントロール)
半分の長さにカットした生葉300gを、3000g量の熱水(100℃)で3分間ブランチングし、水切り後にローラー式破砕機で5mm片程度に粗砕した。
次に、原料と同量の300gの水を加え、50℃ウォーターバスにて15分間攪拌して酵素処理した後、遠心分離機にて3000rpm10分間の処理を行い、1mm篩で濾過し、得られた野菜エキスの粘度測定を行った。
粘度測定は、約25℃にてB型粘度計を用いて測定した。
(Control)
300 g of fresh leaves cut into half length were blanched for 3 minutes with 3000 g of hot water (100 ° C.), and after draining, they were roughly crushed to about 5 mm pieces with a roller crusher.
Next, 300 g of water equal to the amount of the raw material was added, and the mixture was stirred for 15 minutes in a 50 ° C. water bath, treated with enzyme, then processed at 3000 rpm for 10 minutes in a centrifuge, and filtered through a 1 mm sieve. The viscosity of the vegetable extract was measured.
Viscosity was measured at about 25 ° C. using a B-type viscometer.
(実施例2及び3)
半分の長さにカットした生葉300gを、3000g量のクエン酸0.05%溶液(100℃)で3分間ブランチングし、水切り後にローラー式破砕機で5mm片程度に粉砕した。
次に、原料と同量の300gの水を加え、50℃のウォーターバスにて品温が42〜45℃になるまで加温した後、酵素(ポリガラクツロナーゼ:0.2質量%、βグルカナーゼ:0.1質量%、グルコアミラーゼ:0.1質量%を含む)を添加し、50℃ウォーターバスにて15分間攪拌して酵素処理した後、遠心分離機にて3000rpm10分間の処理を行い、1mm篩で濾過し、得られた野菜エキスの粘度測定を行った。
粘度測定は、約25℃にてB型粘度計を用いて測定した。
なお、各酵素の量(質量%)は原料生葉に対する質量割合である。
(Examples 2 and 3)
300 g of fresh leaves cut in half length were blanched with a 3000 g amount of 0.05% citric acid solution (100 ° C.) for 3 minutes, drained and crushed to about 5 mm pieces with a roller crusher.
Next, 300 g of the same amount as the raw material was added and heated in a 50 ° C. water bath until the product temperature reached 42 to 45 ° C., and then the enzyme (polygalacturonase: 0.2 mass%, β Glucanase: 0.1% by mass, glucoamylase: 0.1% by mass) was added, and the mixture was stirred for 15 minutes in a 50 ° C. water bath and treated with an enzyme at 3000 rpm for 10 minutes. It filtered with a 1 mm sieve, and the viscosity of the obtained vegetable extract was measured.
Viscosity was measured at about 25 ° C. using a B-type viscometer.
In addition, the quantity (mass%) of each enzyme is a mass ratio with respect to raw raw leaves.
オクラやツルムラサキなど、モロヘイヤ以外の粘性含有野菜に適用しても粘性低下効果が認められた。特にオクラに関しては、搾汁液の粘度が約1/4まで低下した。
また、搾汁液の回収率(歩留り)は、酵素処理によりオクラでは22%、ツルムラサキでは15%も増加した。また、搾汁液のBxもコントロールに比較して増加した。
Even when applied to viscous vegetables other than moroheiya, such as okra and tsurumurasaki, a viscosity reducing effect was observed. Especially for okra, the viscosity of the juice was reduced to about 1/4.
Moreover, the recovery rate (yield) of the juice was increased by 22% for okra and 15% for Tsurmurasaki by enzyme treatment. Moreover, Bx of the juice was also increased compared to the control.
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