JP5098198B2 - Copper compound composition - Google Patents

Description

  The present invention relates to a copper compound blended composition with reduced unpleasant taste when drinking, and can be used in the fields of medicine and food.

  The composition containing a copper compound was difficult to drink due to an unpleasant taste derived from copper ions. This taste is the same as the taste when using an astringent that binds to proteins such as tannin and alum (astringent taste).

  Until now, in order to improve the unpleasant taste of a copper compound, the technique of mix | blending an oleoresin and an accent fragrance | flavor is disclosed (patent document 1). However, these are not sufficient in improving the unpleasant taste derived from copper ions.

Patent No. 2580648

  An object of the present invention is to provide a copper compound blended composition that reduces the astringent taste derived from copper ions, is easy to drink, and does not leave an unpleasant aftertaste.

  In order to solve the above-mentioned problems, the present inventors have conducted extensive studies and found that the astringency of copper ions is improved when a carnitine compound is added to a copper compound, thereby completing the present invention.

The present invention has been completed based on such findings, and includes the following inventions.
(1) A composition comprising a copper compound and carnitines.
(2) The copper compound is one or more compounds selected from the group consisting of copper gluconate, copper sulfate, copper citrate, copper chloride, copper nitrate, and copper phosphate. Composition.
(3) The composition according to the above (1) or (2), wherein the carnitines are carnitine salts.
(4) The above (1) to (1), further comprising one or more amino acids selected from the group consisting of acidic amino acids and amides thereof, basic amino acids, γ-aminobutyric acid and salts thereof. The composition according to any one of 3).
(5) The composition according to any one of (1) to (4), wherein the composition is a composition for internal use liquid and has a pH of 2.5 to 7.0.
(6) A method for reducing an unpleasant taste of a copper compound blended composition, which comprises blending carnitines.

  According to the present invention, it is possible to provide a copper compound blended composition that reduces the astringent taste derived from copper ions, is easy to drink, and does not leave an unpleasant aftertaste.

  This copper compound blended composition can be applied to various beverages such as various preparations including syrups, drinks and other pharmaceuticals and quasi-drugs, and nutritional functional foods.

  The copper compound in the present invention is a salt with an organic acid or inorganic acid containing copper as a cation component, and these organic acid and inorganic acid may be non-toxic, and examples of the organic acid include gluconic acid. Citric acid, tartaric acid, or inorganic acids include, for example, sulfuric acid, hydrochloric acid, nitric acid, phosphoric acid, and carbonic acid.

  Examples of the copper compound to be blended include copper gluconate, copper sulfate, copper citrate, copper chloride, copper nitrate, and copper phosphate. These may be blended singly or in combination of two or more.

  The compounding quantity of a copper compound changes with the intended purposes of the copper compound compounding composition which mix | blends this. In terms of nutritional intake, 0.1 to 10 mg per day is preferable in terms of copper ions. When taken as a 100 mL oral liquid solution per day, the copper ion concentration is 0.0001 to 0.01 W. / V%.

  Carnitines in the present invention include carnitine, carnitine salts (carnitine hydrochloride, carnitine nitrate, carnitine fumarate, carnitine tartrate, etc.), carnitine analogues (acylcarnitine, such as acetylcarnitine, propionylcarnitine, etc.), etc. is there. Among these, a carnitine salt is preferable, and carnitine hydrochloride, carnitine fumarate, carnitine tartrate, and the like can be particularly preferable. One or more of these carnitines can be selected and blended.

  The compounding ratio of the copper compound and the carnitines can be appropriately adjusted depending on the dosage form to be administered. For example, when the composition of the present invention is used as a composition for internal use, its copper ion 1 It is 0.1 mass part or more with respect to a mass part, Preferably it is 1-20000 mass part, More preferably, it is 5-10000 mass part.

  In the present invention, the effects of the present invention can be enhanced by further blending specific amino acids.

  Specific amino acids in the present invention include acidic amino acids (such as aspartic acid and glutamic acid) and amides thereof (such as asparagine and glutamine), basic amino acids (such as arginine, histidine and lysine), γ-aminobutyric acid and salts thereof Can be mentioned.

  Examples of the amino acid salt include salts of amino acids with acids (hydrochloric acid, sulfuric acid, methanesulfonic acid, etc.), bases (ammonia, sodium hydroxide, potassium hydroxide, calcium hydroxide, magnesium hydroxide, etc.) or other amino acids. Can be mentioned.

  In the present invention, one or more of these specific amino acids can be selected and blended.

  The γ-aminobutyric acid used in the present invention is not limited to synthetic products, but is also derived from plants such as rice germ, rice bran, tea and nanban, and those derived from microorganisms such as koji mold, lactic acid bacteria, yeast and chlorella. be able to.

  The compounding ratio of the copper compound and the specific amino acids can be appropriately adjusted depending on the dosage form to be administered. For example, when the composition of the present invention is used as a composition for internal use, the copper It is 0.1 mass part or more with respect to 1 mass part of ion, Preferably it is 1-20000 mass part, More preferably, it is 5-10000 mass part.

  In the case of using the copper compound blend composition of the present invention as an internal liquid, a desired effect can be obtained by adjusting the pH to 2.5 to 7.0. However, because of ease of drinking, the pH is 3.0. It is preferable to adjust to ~ 5.5.

  The pH of the liquid is, for example, an organic acid such as citric acid, malic acid, fumaric acid, tartaric acid, lactic acid or succinic acid, a salt of these organic acids, an inorganic acid such as phosphoric acid or hydrochloric acid, or an inorganic base such as sodium hydroxide. Can be adjusted.

  Vitamins, minerals, other amino acids, herbal medicines, herbal extracts, caffeine, royal jelly, and the like can be appropriately blended in the copper compound-blending composition of the present invention as long as the effects of the present invention are not impaired. Moreover, additives such as antioxidants, colorants, flavors, flavoring agents, surfactants, solubilizers, preservatives, sweeteners, and the like can be appropriately blended as necessary so long as the effects of the present invention are not impaired.

  The method for preparing the copper compound blend composition of the present invention is not particularly limited. Usually, after each component is dissolved in an appropriate amount of purified water, the pH is adjusted, the remaining purified water is added to adjust the volume, and if necessary, filtration and sterilization are performed to obtain the intended copper compound blend composition. It is done.

  Hereinafter, the present invention will be described in more detail with reference to examples and test examples. An example is an example which shows an embodiment of the invention concretely, and a test example is an example of a test which evaluated an example. The present invention is not limited to these examples.

Example 1
Copper gluconate 0.004g
Zinc gluconate 0.02g
Carnitine chloride 0.02g
Arginine hydrochloride 0.20g
0.25 g of magnesium aspartate
Calcium gluconate 0.50g
0.01 g of thiamine nitrate
Riboflavin sodium phosphate 0.01 g
0.01 g of pyridoxine hydrochloride
Ascorbic acid 1.00g
Aminoethylsulfonic acid 2.00g
Xylitol 4.00g
Trehalose 5.00g
Erythritol 5.00g
Citric acid 0.80 g
Sodium citrate appropriate amount Sodium benzoate 0.06g
0.10g mixed fruit flavor
After dissolving the above components in purified water, the pH was adjusted to 3.0, and purified water was added to make up a total volume of 100 mL. This solution was filtered with a filter paper, and the filtrate was sterilized by heating at 80 ° C. for 25 minutes using a sterilizer, and then filled into a glass bottle and capped to obtain an internal solution.

Example 2
Copper gluconate 0.007g
Zinc gluconate 0.05g
Carnitine tartrate 0.20g
Leucine 0.20g
Isoleucine 0.20g
Valine 0.20g
Histidine hydrochloride 0.10g
Arginine hydrochloride 0.20g
Calcium gluconate 0.40g
Magnesium aspartate 0.30 g
0.01 g of thiamine nitrate
Riboflavin 0.01g
0.10 g of pyridoxine hydrochloride
Ascorbic acid 1.00g
Aminoethylsulfonic acid 1.00g
Sorbitol 4.00g
Trehalose 5.00g
Xylitol 4.00g
Stevia extract 0.03g
Acesulfame potassium 0.03g
Malic acid 0.10g
Citric acid 0.40g
Sodium citrate appropriate amount Benzoic acid 0.06g
Butyl paraoxybenzoate 0.006g
Propyl paraoxybenzoate 0.006 g
Apple flavor 0.10g
After dissolving the above components in purified water, the pH was adjusted to 4.0, and purified water was added to make up a total volume of 100 mL. This solution was filtered with a filter paper, and the filtrate was sterilized by heating at 80 ° C. for 25 minutes using a sterilizer, and then filled into a glass bottle and capped to obtain an internal solution.

Example 3
0.03 g of copper gluconate
Zinc gluconate 0.08g
Carnitine fumarate 0.20g
γ-aminobutyric acid 0.20 g
Arginine hydrochloride 0.20g
Leucine 0.20g
Isoleucine 0.20g
Valine 0.20g
Riboflavin 0.01g
0.01 g of pyridoxine hydrochloride
Ascorbic acid 1.00g
Cyanocobalamin 120μg
Panthenol 0.01g
Nicotinamide 0.05g
Aminoethylsulfonic acid 1.00g
Sorbitol 5.00g
Trehalose 2.00g
Maltitol 2.00g
Citric acid 0.40g
Sodium malate appropriate amount Benzoic acid 0.06g
Butyl paraoxybenzoate 0.006g
Propyl paraoxybenzoate 0.006 g
0.10g mixed fruit flavor
After dissolving the above components in purified water, the pH was adjusted to 4.5, and purified water was added to make up a total volume of 100 mL. This solution was filtered with a filter paper, and the filtrate was sterilized by heating at 80 ° C. for 25 minutes using a sterilizer, and then filled into a glass bottle and capped to obtain an internal solution.

Example 4
Copper gluconate 0.007g
Zinc gluconate 0.05g
Carnitine tartrate 0.20g
Histidine hydrochloride 0.10g
Arginine hydrochloride 0.20g
Leucine 0.20g
Isoleucine 0.20g
Valine 0.20g
Calcium gluconate 0.80 g
Magnesium aspartate 0.40 g
0.01 g of thiamine nitrate
Riboflavin sodium phosphate 0.01 g
0.01 g of pyridoxine hydrochloride
Nicotinamide 0.10g
Anhydrous caffeine 0.10g
Aminoethylsulfonic acid 2.00g
Yokuinin style extract 2.00mL
Glucose 5.00g
Indigestible dextrin 4.00 g
Erythritol 5.00g
Xylitol 2.00g
Stevia extract 0.02g
Acesulfame potassium 0.03g
Sucralose 0.005g
Citric acid 0.80 g
Sodium citrate appropriate amount Sodium benzoate 0.06g
Butyl paraoxybenzoate 0.006g
Propyl paraoxybenzoate 0.006 g
0.10g mixed fruit flavor
After dissolving the above components in purified water, the pH was adjusted to 3.5, and purified water was added to make up a total volume of 100 mL. This solution was filtered with a filter paper, and the filtrate was sterilized by heating at 80 ° C. for 25 minutes using a sterilizer, and then filled into a glass bottle and capped to obtain an internal solution.

Example 5
0.03 g of copper gluconate
Zinc gluconate 0.08g
Carnitine fumarate 0.20g
Glutamine 0.20g
Leucine 0.20g
Isoleucine 0.20g
Valine 0.20g
γ-aminobutyric acid 0.20 g
Arginine hydrochloride 0.20g
Histidine hydrochloride 0.10g
Calcium gluconate 0.20g
Calcium lactate 0.10g
Sodium aspartate 0.10g
Magnesium aspartate 0.20 g
0.01 g of thiamine nitrate
Riboflavin sodium phosphate 0.02g
0.03 g of pyridoxine hydrochloride
Nicotinamide 0.05g
Anhydrous caffeine 0.10g
Aminoethylsulfonic acid 2.00g
Yokuinin style extract 2.00mL
Royal jelly 0.60g
Glucose 5.00g
Sorbitol 5.00g
Xylitol 5.00g
Stevia extract 0.02g
Acesulfame potassium 0.03g
Citric acid 0.80 g
Sodium citrate 0.10g
Phosphoric acid 0.30 g
Hydrochloric acid appropriate amount Sodium benzoate 0.06g
Butyl paraoxybenzoate 0.006g
Propyl paraoxybenzoate 0.006 g
0.10g mixed fruit flavor
After dissolving the above components in purified water, the pH was adjusted to 3.5, and purified water was added to make up a total volume of 100 mL. This solution was filtered with a filter paper, and the filtrate was sterilized by heating at 80 ° C. for 25 minutes using a sterilizer, and then filled into a glass bottle and capped to obtain an internal solution.

Test example
Hagerman et al. Reported that protein (bovine serum albumin: BSA) in solution was aggregated by tannin, and the amount of precipitation was proportional to the amount of tannin ( J. Agric . Food . Chem ., 1978, Vol. 26 , 809) . -812). The present inventors have found that when a BSA solution is added to a copper ion solution, the solution is suspended, the amount of light transmission decreases, and the decrease in the amount of transmission correlates with the concentration of copper ions.

Test method (1) Preparation of BSA solution: 6 g of BSA (Sigma Chemical Co .; fraction V, fatty acid free) is dissolved in an appropriate amount of purified water, 100 mg of citric acid is added, and the pH is adjusted with NaOH solution (1 mol / L). Adjusted to 4.8 and made up to 100 mL with purified water.

  (2) Preparation of diluting solution: 100 mg of citric acid was dissolved in an appropriate amount of water, pH was adjusted to 4.8 with NaOH solution (1 mol / L), and purified water was adjusted to 100 mL.

  (3) Preparation of copper ion solution: To 0.02 g, 0.11 g and 0.18 g of copper gluconate, 0.10 g of citric acid was added. Each was dissolved in an appropriate amount of purified water, adjusted to pH 4.8 with NaOH solution (1 mol / L), and made up to 100 mL with purified water.

(4) Evaluation of protein-copper ion interaction (convergence) 6 mL of BSA solution was added to 2 mL of each copper ion solution, and the total volume was adjusted to 10 mL with a diluted solution. This was shaken at 40 ° C. for 30 minutes. Using a quartz cell (L = 1 cm), the absorbance at 500 nm, which is a wavelength that is not absorbed by each transparent solution, was measured with a spectrophotometer (manufactured by Hitachi, Ltd .: U-3300). This result (FIG. 1) shows that the copper ion concentration and the absorbance are correlated.

  It was confirmed that the results of sensory evaluation of the astringent taste of copper ion solutions having various concentrations were correlated with the absorbance when the BSA solution was added to the copper ion solution. The sensory evaluation was performed as B when the astringent taste was strongly unacceptable, A4 to A1 according to the strength of the astringent taste, and A when the astringent taste was not felt at all. The results are shown in FIG.

  From these facts, when the absorbance when a copper ion solution added with a certain substance is mixed with a BSA solution decreases compared to the case where no addition is made, the addition of that substance causes the aggregation (convergence) due to protein-copper ion interaction. ) Is reduced, that is, the astringency is reduced.

(5) Protein aggregation in Examples and Comparative Examples After dissolving the components shown in Table 1 in purified water, adjust the pH to 4.8, add purified water to make a total volume of 100 mL, and prepare samples 1 to 5 did. The obtained sample was evaluated for protein aggregation according to the methods described in the test methods (1) to (4). The results are shown in Table 1.

  From the results in Table 1, it can be seen that the absorbance of Samples 1 to 4 containing the carnitines of the present invention is smaller than that of Sample 5 containing no carnitines. It became clear that protein aggregation, that is, astringency, is reduced by adding carnitines.

  In addition, it can be seen that the absorbance of Samples 2 to 4 in which specific amino acids are further mixed with carnitines is smaller than that of Sample 1 in which specific amino acids are not mixed. It has been clarified that the protein aggregation property, that is, the astringency is further reduced by adding specific amino acids.

  Furthermore, from Table 1 and FIG. 2, it was revealed that the absorbance values shown in the samples 1 to 4 belong to a range that can be used without worrying about the astringency. Further, in actuality, specimens 1 to 5 were prepared by removing the BSA solution according to the method described in the test method (4) above, and designated specimens 6 to 10, respectively. Based on the evaluation criteria described above, 6 people were evaluated as a panelist and the astringent taste was evaluated. The results are shown in Table 2 below. As is clear from Table 2, Samples 6 to 9 were easier to drink than Sample 10, and had no unpleasant aftertaste.

FIG. 1 shows the correlation between protein aggregation and copper ion concentration. FIG. 2 shows the correlation between absorbance and sensory evaluation.

According to the present invention, although it is a composition containing a copper compound, it can be taken without feeling the unpleasant taste of the copper compound. For example, pharmaceuticals such as syrups and drinks And various beverages such as various preparations including quasi-drugs and quasi-drugs.

Claims (3)

Copper gluconate, and one or two compounds selected from the group consisting of carnitine chloride and carnitine fumarate, and one or two amino acids selected from the group consisting of sodium aspartate and γ-aminobutyric acid A composition for internal use liquid, characterized by being formulated. The composition for internal use according to claim 1 , wherein the pH is 3.0 to 4.5. 1 or 2 compounds selected from the group consisting of carnitine chloride and carnitine fumarate, and 1 or 2 amino acids selected from the group consisting of sodium aspartate and γ-aminobutyric acid , The method of reducing the unpleasant taste of the composition for internal use liquid containing copper gluconate.

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