JP4802925B2 - Analytical device and analytical apparatus using the same - Google Patents

Description

  The present invention relates to an analysis device for optically analyzing a biological fluid, and an analysis apparatus using the analysis device. More specifically, the present invention relates to a component measurement of a biological fluid using an optical analysis device. The method for collecting solution components or solid components in the analytical device to be used is specifically a method for collecting plasma components or blood cell components in blood.

  2. Description of the Related Art Conventionally, as a method for optically analyzing a biological fluid, a method for analyzing using a microdevice having a liquid flow path is known. Microdevices can control fluids using a rotating device, and can measure sample solutions, separate solid components, and transfer and distribute separated fluids using centrifugal force. Various biochemical analyzes can be performed.

As a method of transferring the sample solution using centrifugal force, as shown in FIG. 23, the sample solution is connected to the large fluid chamber 81 and the large fluid chamber 81 and outward in the radial direction with respect to the large fluid chamber 81. The measuring chamber 82 arranged, the overflow chamber 83 connected to the measuring chamber 82, the receiving chamber 84 arranged radially outward with respect to the measuring chamber 82, and liquid from the measuring chamber 82 to the receiving chamber 84. a capillary connecting means 85 for supplying, there is rotation analysis device having.

Capillary connecting means 85 includes a siphon 86 which has a capillary structure, elbow-like bent portion of the siphon 86 consists of the center of the device for rotating analysis, the radially innermost point substantially the same distance of the metering chamber 82 has been positioned such, because the rotation of the capillary force of the rotating analyzing device is smaller than the centrifugal force, the interface of the liquid / air has the same axis as the axis of the device for rotating the analysis, and rotated analyzing device Consistent with the shape of the rotating cylinder having a radius equal to the distance from the center of the chamber to the radially innermost point of the metering chamber 82, the metering chamber 82 is filled with sample solution, and excess sample solution is It flows into the overflow chamber 83.

When stopping the device for rotating the analysis, the sample solution filled in the metering chamber 82 flows into the capillary connecting means 85 in the capillary force, by rotating again, siphon is started, present in the metering chamber 82 the liquid Is discharged into the receiving chamber 84 (Patent Document 1).

At this time, if there is a solid component in the sample solution, centrifugation is performed in the measuring chamber 82 or the receiving chamber 84 to precipitate the solid component, and then a capillary having a siphon structure is connected to a portion located inward in the radial direction. In this case, only the solution components in the sample solution can be transferred to the next process.
Japanese National Patent Publication No. 5-508709

  However, in the conventional configuration, after centrifugation, only the solution component in the sample solution can be transferred by adjusting the position where the capillary tube is connected, but the solid component is precipitated in the outer circumferential direction. In the transfer by siphon, there is a problem that a solid component or a high concentration solid component solution cannot be transferred.

  In addition, when only the solution component is transferred by a capillary having a siphon structure, the remaining solution flows into the capillary again when the rotation stops, and the solution in the capillary is transferred again by the next rotation. There was also a problem that the measurement accuracy was affected by variations in quantity and the inflow of solid components into the capillaries.

  The present invention solves the above-mentioned conventional problems, and can transfer a solid component or a high-concentration solid component solution, and when a part of the sample solution is transferred, the remaining solution is followed. It is an object of the present invention to provide an analysis device capable of preventing the inflow of the liquid and an analysis apparatus using the same.

In order to solve the conventional problems, partial析用device of the present invention, the sample solution to be analyzed is accommodated in the sample solution analyzing device which can transfer the internally the sample solution, a solution component , A separation chamber for separating the solid component using a centrifugal force generated by rotation of the analytical device, and a part of the solid component separated in the separation chamber is transferred and held A holding flow path for connecting, and an overflow flow path provided between the holding flow path and the separation chamber and connected by a connection passage for transferring the sample solution in the separation chamber, After the solution component separated in the separation chamber and existing in the connection passage preferentially flows into the overflow channel, the solid component separated in the separation chamber flows into the overflow via the connection passage. The channel is filled and then The solid component of the separation chamber, flows from the connecting passage to the holding channel via the overflow channel, a portion of the solid components are retained, that is obtained by it said.

Min析用device of the present invention, in the analysis device, the overflow chamber filled sample solution is discharged into the overflow passage, further wherein the overflow chamber via the coupling portion The overflow solution is connected to the overflow channel, and the transfer of the sample solution from the connection channel to the overflow channel is performed by capillary force, and the overflow channel and the overflow chamber In the meantime, an opening area of the overflow channel in the coupling portion is larger than an opening area of the holding channel.

Min析用device of the present invention, in the analysis device, the overflow chamber filled sample solution is discharged into the overflow passage, further wherein the overflow chamber via the coupling portion It is connected to the overflow channel, and the transfer of the sample solution from the connection channel to the overflow channel is performed by pressure feeding, from an air hole provided in the overflow chamber, Air in the overflow channel and the connecting passage is sucked by the suction pump, and the solution component existing in the connecting passage is preferentially caused by the pressure difference generated by the suction, and the overflow in the overflow channel It is transferred to the said joint part with a chamber, It is characterized by the above-mentioned.

Min析用device of the present invention, in the analysis device, communicating with the holding passage, and the solid component, a mixing chamber for mixing the dilute solution or reagent solution, communication with the mixing chamber, And a diluent storage chamber for storing the diluent solution or the reagent solution.

Min析用device of the present invention, in the analysis device, the holding passage, weighing the solid component by the volume of the flow path, it is characterized in.

Analysis apparatus of the present invention is an analysis apparatus in which the analysis device is mounted, the analyzing device comprises a rotary drive means for rotating about its axis, the sample solution accommodated in the separation chamber The sample solution is separated into a solution component and a solid component by rotating the analysis device in a state where the analysis device is rotated, and then the solution component that has flowed into the connection passage from the separation chamber by stopping the rotation of the analysis device. Is transferred in preference to the connecting portion of the overflow channel with the overflow chamber by capillary force, and then the solid component in the separation chamber is transferred to the holding channel by capillary force, and again, By rotating the analytical device, the solution component and the solid component filled in the overflow channel and the coupling portion thereof are discharged into the overflow chamber.

Analysis apparatus of the present invention is an analysis apparatus in which the analysis device is mounted, a rotary drive means for rotating the device for the analysis about the axis, for sucking the liquid of the analysis within the device A pump, and a coupling mechanism for connecting the pump and an air hole of the analytical device, and rotating the analytical device in a state where the sample solution is accommodated in the separation chamber, After the sample solution is separated into a solution component and a solid component, and then the rotation of the analysis device is stopped, the connection mechanism is connected to the air hole of the overflow chamber, and air is sucked by the pump. Then, the solution component that has flowed into the connecting passage is preferentially transferred to the coupling portion of the overflow channel with the overflow chamber, and after the coupling portion is filled, the holding channel Linked to The coupling mechanism is connected to the air hole of the chamber, and the solid component in the separation chamber is transferred to the holding channel by sucking air with the pump, and the holding channel is filled with the solid component. Thereafter, the coupling mechanism is detached, and the analysis device is rotated again, so that the solution component and the solid component filled in the overflow channel and the coupling portion thereof are discharged into the overflow chamber. It is what.

Analysis apparatus of the present invention is an analysis apparatus in which the analysis device is mounted, a rotary drive means for rotating the device for the analysis its axis around the sample solution is contained in a solution housing chamber Further, by rotating the analytical device, the sample solution is transferred to the outer periphery of the liquid storage chamber, and then the rotation of the analytical device is stopped by capillary force from the liquid storage chamber. The sample solution is sucked out and held in the holding channel, and further, the sample solution is sucked out and held in the capillary channel having a siphon structure connected to the sample overflow chamber by capillary force from the liquid storage chamber. Then, by rotating the analysis device, the sample solution filled in the holding channel is transferred to the liquid holding chamber, and the support in the liquid containing chamber is transferred. Pull solution, by siphon structure of the capillary channel, wherein to discharge the sample overflow chamber, it is characterized in.

Analysis apparatus of the present invention is an analysis apparatus in which the analysis device is mounted, a rotary drive means for rotating the device for the analysis about the axis, for transporting liquid of the analysis within the device A gas introduction mechanism is provided, and the analysis device in which the sample solution is stored in the solution storage chamber is rotated to transfer the sample solution to the outer periphery of the liquid storage chamber, and then the analysis device is rotated. By stopping, the siphon structure that sucks and holds the sample solution from the liquid storage chamber to the holding channel by capillary force, and is connected to the sample overflow chamber by capillary force from the liquid storage chamber. The sample solution is sucked out and held in the capillary channel having the gas introduction mechanism, the air hole provided in the liquid separation position of the holding channel, The sample solution filled in the holding flow path is connected to the air hole provided in the inner peripheral portion of the body storage chamber, and the gas is introduced from the gas introduction mechanism through the air hole. It is separated at the liquid separation position and transferred to the liquid holding chamber, and the sample solution in the liquid storage chamber is discharged into the sample overflow chamber by the siphon structure of the capillary channel.

  According to the analysis device and the analysis apparatus of the present invention, the solid component after centrifugation or the high-concentration solid component solution can be transferred in a necessary amount. In addition, when a part of the sample solution is transferred, it is possible to prevent the remaining solution from flowing in later, and the measurement accuracy of the analytical device can be improved.

Hereinafter, embodiments of the analyzing device of the present invention will be described in detail with reference to the drawings.
(Embodiment 1)
Hereinafter, the analysis device 101 according to the first embodiment corresponding to the first, second, and fifth aspects of the present invention and the analysis apparatus 1000 using the same will be described with reference to FIGS. To do.

FIG. 1 is a schematic diagram showing a configuration of an analysis device 101 according to Embodiment 1 of the present invention. In Figure 1, the analysis device 101 according to the first embodiment, the micro-channel 4a, a substrate 1 having 4b, a flat substrate 2, the substrate 1 and 2 of both, the adhesive layer 3 bonded to each other It consists of Also, 5 is the reaction solution filled in the micro-channel 4a of the micro channel formed by laminating the substrates 1 and 2 of both.

  The microchannels 4a and 4b formed on the substrate 1 are produced by injection-molding a microchannel pattern having projections and depressions. A sample solution to be analyzed is injected into the analysis device 101, and the microchannel 4a The sample solution can be transferred inside through 4b.

  In the first embodiment, the microchannel 4a is irradiated with transmitted light 6a to optically analyze the reaction state between the sample solution to be examined and the reagent. At the time of measurement, the reaction solution 5 obtained by reacting the sample solution and the reagent is filled in the microchannel 4a, and the absorbance of the reaction solution 5 changes depending on the reaction rate of the sample solution and the reagent. By irradiating the channel 4a with transmitted light and measuring the amount of the transmitted light at the light receiving unit 7, the change in the amount of light transmitted through the reaction solution 5 can be measured, thereby analyzing the reaction state. Can do.

  In the first embodiment, the thickness of the substrate 1 and the substrate 2 is 1 mm to 5 mm. However, this is not particularly limited as long as the microchannels 4a and 4b can be formed. Good. Further, the shape of the substrate 1 and the substrate 2 is not particularly limited, and a shape according to the purpose of use, for example, a disk shape, a fan shape, a sheet shape, a plate shape, a rod shape, or other complicated shapes. It is possible to take a shape such as

  In the first embodiment, plastic is used as the material of the substrate 1 and the substrate 2 from the viewpoint of easy moldability, high productivity, and low cost, but this includes glass, silicon wafer, metal, ceramic, etc. Any material that can be joined is not particularly limited.

  In the first embodiment, the substrate 1 and the substrate 2 having the microchannels 4a and 4b are subjected to a hydrophilic treatment in the microchannels 4a and 4b as necessary when the solution is transferred using capillary action. If this is performed, the viscous resistance in the microchannel can be reduced to facilitate fluid movement.

  In the first embodiment, since the plastic material is used, the surface is subjected to hydrophilic treatment. However, a hydrophilic material such as glass is used, or a hydrophilic property such as a surfactant, a hydrophilic polymer, or silica gel is used during molding. A hydrophilic agent such as powder may be added to impart hydrophilicity to the material surface. Examples of the hydrophilic treatment method include a surface treatment method using an active gas such as plasma, corona, ozone, and fluorine, and a surface treatment with a surfactant. Here, the hydrophilic property means that the contact angle with water is less than 90 degrees, and more preferably, the contact angle is less than 40 degrees.

In the first embodiment, the substrate 1 and the substrate 2 are bonded using an adhesive, but both the substrates 1 and 2 are bonded by a bonding method such as fusion bonding or anodic bonding depending on the materials used. You may do it.

FIG. 2 is a schematic diagram showing a configuration of the analysis apparatus 1000 to which the analysis device 101 according to the first embodiment is attached.
In FIG. 2, the analysis device 101 according to the first embodiment is mounted on a motor 102 that is a rotation driving unit of the analysis apparatus 1000 according to the first embodiment, and the analysis is performed by driving the motor 102. Device 101 can be rotated about an axis. The analytical device 101 can transfer or centrifuge the liquid in the device using centrifugal force generated by rotation. In the first embodiment, the disk-shaped analysis device is mounted on the analyzer, but a plurality of fan-shaped or other shapes may be mounted simultaneously.

  Further, the analysis apparatus 1000 irradiates the analysis device 101 with laser light from the laser light source 103 while the analysis device 101 is rotationally driven in the C direction by the motor 102. The laser light source 103 is screwed into a feed screw 105 driven by a traverse motor 104, and the servo control circuit 106 drives the traverse motor 104 so that the laser light source 103 can be arranged at an arbitrary measurement location. The laser light source 103 can be moved in the radial direction.

Above the analysis device 101, a photodetector 107 that detects the amount of transmitted light that has passed through the analysis device 101 out of the laser light emitted from the laser light source 103, and an adjustment circuit 108 that adjusts the gain of the output of the photodetector 107. The A / D converter 109 for A / D converting the output of the adjustment circuit 108, the transmitted light amount signal processing circuit 110 for processing the A / D converted data, and the data obtained by the transmitted light amount signal processing circuit 110 are stored. memory over 113, and a display unit 112 for displaying the results CPU 111, and analyzed for controlling these.

Next, the microchannel configuration of the analysis device 101 of the first embodiment and the sample solution transfer process will be described in detail.
FIG. 3 is a plan view showing a microchannel configuration of the analysis device 101 according to the first embodiment. 4 (a) and 4 (b) are diagrams for explaining the injection / separation process of the analytical device 101 of the first embodiment, and FIGS. 5 (a), (b) and (c) are the present embodiment. It is a figure for demonstrating the measurement process of the analysis device 101 of the form 1, and the filling process of the measurement cell 28. FIG.

  As shown in FIGS. 3, 4, and 5, the microchannel configuration of the analysis device 101 according to the first embodiment has a liquid storage chamber 9 for injecting / accommodating a necessary amount of sample solution for analysis. A separation chamber 10 for separating the sample solution into a solution component and a solid component using a centrifugal force generated by the rotation of the analytical device, and a part of the solid component separated in the separation chamber 10 The holding flow path 13 for transporting and holding it, and the overflow flow path connected between the holding flow path 13 and the separation chamber 10 by the connection passage 11 for transferring the sample solution in the separation chamber 10 12, the overflow chamber 15 from which the sample solution filled in the overflow channel 12 is discharged, and the solid component filled in the holding channel 13 are held, and the solid component and the reagent are mixed / reacted. The absorbance and turbidity of the mixed liquid It is composed of, or the measuring cell 28 for measuring the number of cells.

  Here, in the first embodiment, a reagent for reacting with a solid component is carried in the measurement cell 28. Although omitted in the first embodiment, a reagent reaction chamber for reacting a sample solution with a reagent, a stirring chamber for stirring, and the like are provided between the holding channel 13 and the measurement cell 28. May be.

  In the first embodiment, the depths of the liquid storage chamber 9, the separation chamber 10, the overflow chamber 15, and the measurement cell 28 are formed in a range of 0.3 mm to 2 mm. Can be adjusted according to the conditions for measuring (optical path length, measurement wavelength, reaction concentration of sample solution, type of reagent, etc.).

  The liquid storage chamber 9 is connected to the separation chamber 10, and as shown in FIG. 4A, a pre-weighed amount of sample solution is injected / stored from the injection port 8, and the analysis device 101 is rotated. By generating the centrifugal force, the sample solution can be transferred to the separation chamber 10 as shown in FIG.

  Furthermore, in this Embodiment 1, although the measurement function for hold | maintaining a fixed amount of sample solutions in the separation chamber 10 is not provided, in order to reduce the process before sample solution injection, a sample solution is measured in the separation chamber 10. For the measurement function, for example, when the analysis device is rotated, a configuration in which excess liquid flows out from the liquid level position that can hold the required amount of the separation chamber to the overflow chamber via the overflow channel, or separation A capillary channel formed to communicate from the chamber to the outside of the analytical device is provided, the sample solution is sucked by the capillary force of the capillary channel, the sample solution is measured by the volume of the capillary channel, and the centrifugal force is used. A configuration may be provided in which the sample solution in the capillary channel is transferred to the separation chamber.

  In the first embodiment, the liquid storage chamber 9 and the separation chamber 10 are connected at the same depth, but in order to prevent the sample solution from flowing into the separation chamber 10 during injection, air holes are provided in the separation chamber 10. It may be provided and connected by a capillary channel having a depth of 50 μm to 200 μm.

The separation chamber 10 is connected to the overflow channel 12 through the connection passage 11 from the radially outermost position of the separation chamber 10.
The connection passage 11 is formed with a passage width of 0.5 mm to 2 mm and a depth of 50 μm to 200 μm. This is because the capillary solution generated when the rotation of the analytical device is stopped causes a sample solution in the connection passage 11. If it can be filled, it will not be limited to this.

  In the configuration of the first embodiment, when the analysis device 101 is rotated and the sample solution is transferred from the liquid storage chamber 9 to the separation chamber 10, the connection position between the connection passage 11 and the overflow channel 12. In order to prevent the sample solution from flowing out of the separation chamber 10, the size of the separation chamber 10, the connection position of the connection passage 11 and the overflow channel 12, etc. are optimized from the amount of the sample solution weighed in advance. Therefore, it is necessary to prevent the sample solution from flowing out. In the first embodiment, since the connection passage 11 is formed to the inner peripheral position from the liquid surface when the separation chamber 10 holds a certain amount of sample solution in this respect, FIG. As shown, the sample solution transferred from the liquid storage chamber 9 by centrifugal force is held in the separation chamber 10 and the connection passage 11.

  The sample solution held in the separation chamber 10 can be separated into a solution component 31 and a solid component 32 as shown in FIG. 4B by rotating at a high speed for several minutes. For example, in the case of blood, the rotation speed is set to 4000 rpm to 6000 rpm, and by rotating for 1 minute to 5 minutes, it can be separated into plasma and blood cells or high hematocrit blood (blood with a high ratio of blood cell components). it can.

  The separation chamber 10 is connected to the holding channel 13 via the connecting passage 11 and the overflow channel 12, and the overflow channel 12 is connected to the overflow chamber 15 located in the outer peripheral direction with respect to the overflow channel 12. The holding channel 13 is connected to a measurement cell 28 that is located on the outer circumferential side of the holding channel 13.

  The depths of the holding channel 13 and the overflow channel 12 are 50 μm to 200 μm, and when the analysis device stops rotating, the separated solid component or high concentration in the separation chamber 10 is generated by capillary force. The solid component solution is filled into the holding channel 13 and the overflow channel 12.

  At that time, all of the solution components 31 separated in the connection passage 11 are first transferred to the overflow channel 12 by capillary force, but the overflow chamber 15 is formed deep. The solution component 31 is not transferred into the overflow chamber 15 but is held in the joint 30 between the overflow channel 12 and the overflow chamber 15. Thereafter, the solid component 32 separated in the connection passage 11 and the separation chamber 10 is transferred to the holding passage 13 via the overflow passage 12.

  In the configuration according to the first embodiment, as shown in FIG. 4B, when the sample solution is centrifuged in the separation chamber 10, the inner peripheral portion of the connecting passage 11 that connects the overflow channel 12 and the separation chamber 10. Therefore, when the solution component 31 flows into the holding channel 13 as it is, the concentration of the solid component is lowered, which causes a variation in measurement accuracy. Therefore, in the first embodiment, as shown in FIG. 5A, at the junction of the overflow channel 12 and the holding channel 13, the connecting portion 30 between the overflow channel 12 and the overflow chamber 15 is used. By making the opening area of the overflow channel 12 larger than the opening area of the holding channel 13, the solution component 31 preferentially flows into the overflow channel 12. Here, the ratio of the opening area of the overflow channel 12 to the holding channel 13 is preferably 1.5 to 5 times. If it is 1.5 times or less, the solution component 31 may flow into the holding channel 13. If it is 5 times or more, the area of the overflow channel 12 becomes too large, so the solid component 32 is filled more than necessary. This is because the loss of the solid component 32 may increase.

  In the first embodiment, an overflow channel 12 is provided between the connection channel 11 and the holding channel 13. Further, in such a configuration, at the branch point between the holding channel 13 and the overflow channel 12. By setting the opening areas of the two flow paths to be larger than the opening area of the holding flow path 13, the solution component 31 is preferentially overflowed over the holding flow path 13. It can flow into the flow channel 12. However, it is also possible to provide the overflow channel 12 from the connection channel 11 through the holding channel 13.

  Since the holding flow path 13 and the overflow flow path 12 can measure the solution according to the volume of the flow path, the solid component 32 is an opening of the holding flow path 13 and the overflow flow path 12 having a certain depth. By adjusting the area, it is possible to determine the permissible volume to be retained for each, and to retain the necessary liquid amount. In other words, the allowable volume of the overflow channel 12 is such that it can accommodate all the solution components 31 present in the connecting passage 11 and flows in at least 0.5 times the amount of the solid component 32 of the solution component 31 to be accommodated. By designing so that it can do, it can suppress that the solution component 31 flows into the holding | maintenance flow path 13. FIG.

  The liquid filled in the holding channel 13 rotates the device for analysis and applies centrifugal force, so that air is mixed from the air holes 19 and pressure is applied to the boundary between the holding channel 13 and the overflow channel 12. The connected liquid is broken at the position of the air hole 19, that is, at the boundary between the holding flow path 13 and the overflow flow path 12, and the liquid filled between the air hole 19 and the air hole 20 flows into the measurement cell 28. To do.

  Similarly, the liquid filled in the overflow channel 12 is also broken at the positions of the air hole 18 and the air hole 19, and the liquid filled between the position of the air hole 18 and the position of the air hole 19 is overflowed. The liquid that has flowed into 15 and filled between the separation chambers 10 from the position of the air holes 18 is returned into the separation chamber 10 by centrifugal force.

  After the holding channel 13 is filled in the state shown in FIG. 5B, the solid component held in the holding channel 13 is rotated as shown in FIG. As shown in c), the sample solution is transferred to the measurement cell 28 by centrifugal force, while the sample solution filled in the overflow channel 12 is transferred to the overflow chamber 15.

  The solid component that has flowed into the measurement cell 28 is mixed with the reagent carried in the measurement cell 28 by the acceleration / deceleration of rotation and the diffusion of the liquid while the rotation is stopped, but an external force such as vibration is used. It is also possible to mix them.

  The concentration of the component to be analyzed contained in the solid component mixed with the reagent in the measurement cell 28 can be calculated by measuring the reaction state with the reagent by measuring absorbance or the like. .

According to the analysis device 101 and the analysis apparatus of the first embodiment, the sample solution is stored as the solution component in the analysis device in which the sample solution to be analyzed is stored and the sample solution can be transferred inside. The solid component is separated into the solid component using the centrifugal force generated by the rotation of the analytical device, and a part of the solid component separated in the separation chamber is transferred and retained. A holding channel 13 and an overflow channel 12 provided between the holding channel and the separation chamber and connected by a connecting passage 11 for transferring the sample solution in the separation chamber. And the solution component present in the connecting passage preferentially flows into the overflow channel, and then the solid component separated in the separation chamber is filled into the overflow channel via the connecting passage, and then the separation chamber of the solid Min, the flows from the connecting passage to the holding channel via the overflow channel, and which a part of the solid component is retained, further, the sample solution is filled in the overflow channel in the The overflow chamber 15 is discharged, and the overflow chamber is connected to the overflow channel via the coupling portion 30, and the transfer of the sample solution from the connection channel to the overflow channel is performed as an overflow channel. Since the opening area of the overflow channel at the joint with the overflow chamber is larger than the opening area of the holding channel and is made by capillary force, the opening area of the overflow channel is By setting it to be larger than the opening area, solution components can be discharged into the overflow channel with priority over the holding channel, and the opening areas of the holding channel and overflow channel can be adjusted. Can determine the permissible volume that each channel holds, and the solid components after centrifugation or high-concentration solids The component solution can be transferred to the holding channel required amount, there is an effect that it is possible to improve the measurement accuracy of the analysis device.

(Embodiment 2)
Hereinafter, an analysis device 201 according to a second embodiment corresponding to claims 1, 3, and 5 of the present invention, and a measurement apparatus using the same will be described with reference to FIGS. .
Note that the main configuration of the analysis device 201 according to the second embodiment and the configuration of the analysis apparatus 1000 to which the analysis device is mounted are the same as those in the first embodiment, and the description thereof is omitted here. .

  FIG. 6 is a plan view showing the microchannel configuration of the analysis device 201 of the second embodiment. 7A and 7B are diagrams for explaining the injection / separation process of the analysis device 201 of the second embodiment, and FIGS. 8A, 8B, and 8C are the present embodiment. It is a figure for demonstrating the measurement process of the analysis device 201 of the form 2, and the filling process of the measurement cell 28. FIG.

  As shown in FIGS. 6, 7, and 8, the microchannel configuration of the analysis device 201 according to the second embodiment has a liquid storage chamber 9 for injecting / accommodating a sample solution in an amount necessary for analysis. A separation chamber 10 for separating the sample solution into a solution component and a solid component using a centrifugal force generated by the rotation of the analytical device 201, and one of the solid components separated in the separation chamber 10 Part is transferred, and the overflow channel is connected between the holding channel 13 for holding it and the connecting channel 11 for transferring the sample solution in the separation chamber 10 between the holding channel 13 and the separation chamber 10. The flow path 12, the overflow chamber 15 in which the sample solution filled in the overflow flow path 12 is discharged, and the solid component filled in the holding flow path 13 are held, and the solid component and the reagent are mixed / React to absorb the mixed liquid. Degree, and a measurement cell 28 for measuring the number of turbidity, or cells.

  Here, in the second embodiment, a reagent for reacting with a solid component is carried in the measurement cell 28. Although omitted in the second embodiment, a reagent reaction chamber for reacting a sample solution with a reagent, a stirring chamber for stirring, and the like are provided between the holding channel 13 and the measurement cell 28. May be.

  In the second embodiment, the depth of the liquid storage chamber 9, the separation chamber 10, the overflow chamber 15, and the measurement cell 28 is formed to be 0.3 mm to 2 mm. It can be adjusted according to the conditions for measuring the absorbance (optical path length, measurement wavelength, sample solution reaction concentration, reagent type, etc.).

  The liquid storage chamber 9 is connected to the separation chamber 10, and as shown in FIG. 7A, a pre-weighed amount of sample solution is injected / stored from the injection port 8, and the analysis device 201 is rotated. By generating the centrifugal force, the sample solution can be transferred to the separation chamber 10 as shown in FIG.

  Furthermore, in this Embodiment 2, although the measurement function for hold | maintaining a fixed amount of sample solutions in the separation chamber 10 is not provided, in order to reduce the process before sample solution injection, a sample solution is measured in the separation chamber 10. For the measurement function, for example, when the analysis device is rotated, a configuration in which excess liquid flows out from the liquid level position that can hold the required amount of the separation chamber to the overflow chamber via the overflow channel, or separation A capillary channel formed to communicate from the chamber to the outside of the analytical device is provided, the sample solution is sucked by the capillary force of the capillary channel, the sample solution is measured by the volume of the capillary channel, and the centrifugal force is used. A configuration for transferring the sample solution in the capillary channel to the separation chamber may be provided.

  In the second embodiment, the liquid storage chamber 9 and the separation chamber 10 are connected at the same depth, but in order to prevent the sample solution from flowing into the separation chamber 10 during injection, air holes are provided in the separation chamber 10. The liquid storage chamber 9 and the separation chamber 10 may be connected by a capillary channel having a depth of 50 μm to 200 μm.

The separation chamber 10 is connected to the overflow channel 12 and the holding channel 13 via the connection passage 11 from the radially outermost position of the separation chamber 10.
The connection passage 11 is formed with a passage width of 0.5 mm to 2 mm and a depth of 50 μm to 200 μm, but this is not particularly limited thereto.

  In the configuration of the second embodiment, when the analysis device 201 is rotated and transferred from the liquid storage chamber 9 to the separation chamber 10, the connection position between the connection passage 11 and the overflow channel 12 is exceeded. In order to prevent the sample solution from flowing out of the separation chamber 10, the size of the separation chamber 10, the connection position of the connecting passage 11 and the overflow channel 12, etc. are optimized from the amount of the sample solution weighed in advance. It is necessary to prevent spillage. In the second embodiment, regarding this point, the connection passage 11 is formed to the inner peripheral position from the liquid surface when the separation chamber 10 holds a certain amount of sample solution. As shown, the sample solution transferred from the liquid storage chamber 9 by centrifugal force is held in the separation chamber 10 and the connection passage 11.

  The sample solution held in the separation chamber 10 can be separated into a solution component 31 and a solid component 32 as shown in FIG. 7B by rotating at a high speed for several minutes. For example, in the case of blood, the rotation speed is set to 4000 rpm to 6000 rpm, and by rotating for 1 minute to 5 minutes, it can be separated into plasma and blood cells or high hematocrit blood (blood with a high ratio of blood cell components). it can.

  The overflow channel 12 is connected to an overflow chamber 15 in the outer circumferential direction than the overflow channel 12, and the holding channel 13 is connected to a measurement cell 28 in the outer circumferential direction than the holding channel 13.

  The depth of the holding channel 13 and the overflow channel 12 is 50 μm to 200 μm, and the solution is filled into the holding channel 13 and the overflow channel 12 by suction force.

  In the configuration of the second embodiment, as shown in FIG. 7B, when the sample solution is centrifuged in the separation chamber 10, the inner peripheral portion of the connecting passage 11 that connects the overflow channel 12 and the separation chamber 10. Therefore, when the solution component 31 flows into the holding channel 13 as it is, the concentration of the solid component is lowered, which causes a variation in measurement accuracy. Therefore, in the second embodiment, as shown in FIG. 8A, the air hole 29 provided in the overflow chamber 15 and the suction pump (not shown) are connected by a connecting means (not shown) such as a tube. By driving the suction pump, the air in the overflow channel 12 and the connecting passage 11 is sucked, and the solution component 31 existing in the connecting passage 11 is overflowed by the pressure difference generated by the suction. It is transferred in preference to the connecting part 30 with the chamber 15. Thereafter, a suction pump is connected to the air hole 33 provided in the measurement cell 28 by a connecting means such as a tube, and the solid component 32 in the separation chamber 10 is sucked into the holding channel 13 by similarly sucking from the air hole 33. Can be filled. At this time, the air hole 19 and the air hole 29 are preferably sealed.

  Since the holding flow path 13 and the overflow flow path 12 can measure the solution according to the volume of the flow path, the solid component 32 is an opening of the holding flow path 13 and the overflow flow path 12 having a certain depth. By adjusting the area, the allowable volume is determined, and the necessary liquid amount can be maintained. That is, the allowable volume of the overflow channel 12 can accommodate all the solution components 31 present in the connection passage 11 and can flow in at least 0.5 times the amount of the solid component 32 of the solution component 31 to be accommodated. By designing to, it can suppress that the solution component 31 flows in into the holding | maintenance flow path 13.

  The liquid filled in the holding channel 13 rotates the analysis device 201 and applies centrifugal force, so that air is mixed from the air holes 19 and pressure is applied to the boundary between the holding channel 13 and the overflow channel 12. The connected liquid is broken at the position of the air hole 19, that is, at the boundary between the holding flow path 13 and the overflow flow path 12, and the liquid filled between the air hole 19 and the measurement cell 28 is measured. Flow into.

  Similarly, the liquid filled in the overflow channel 12 is also broken at the position of the air hole 19, and the liquid filled between the overflow chamber 15 from the position of the air hole 19 flows into the overflow chamber 15. Then, the liquid filled between the separation holes 10 from the position of the air holes 19 is returned into the separation chamber 10 by centrifugal force.

  After the holding channel 13 is filled in a state as shown in FIG. 8B, the solid component held in the holding channel 13 is rotated by rotating the analysis device 201 again, as shown in FIG. As shown in c), the sample solution is transferred to the measurement cell 28 by centrifugal force, while the sample solution filled in the overflow channel 12 is transferred to the overflow chamber 15.

  The solid component that has flowed into the measurement cell 28 is mixed with the reagent carried in the measurement cell 28 by the acceleration / deceleration of rotation and the diffusion of the liquid while the rotation is stopped, but an external force such as vibration is used. It is also possible to mix them.

  The concentration of the component to be analyzed contained in the solid component mixed with the reagent in the measurement cell 28 can be calculated by measuring the reaction state with the reagent by measuring absorbance or the like. .

According to such an analysis device 201 of the second embodiment and an analysis apparatus using the same, a centrifugal force generated by rotation of the analysis device is used for the sample solution as the solution component and the solid component. The separation chamber 10 for separating them, a part of the solid component separated in the separation chamber is transferred, and the holding channel 13 for holding it, and between the holding channel and the separation chamber, And an overflow channel 12 connected by a connection passage for transferring the sample solution in the separation chamber, and the solution component 31 separated in the separation chamber and existing in the connection passage flows preferentially into the overflow channel. After that, the solid component 32 separated in the separation chamber is filled into the overflow channel via the connection passage, and then the solid component in the separation chamber passes through the overflow channel from the connection passage. Into the holding channel and The part shall be maintained, with the overflow chamber 15 to the sample solution filled in further overflow channel in is discharged,該溢flow chamber, the overflow channel via the coupling portion 30 The sample solution is transferred from the connected flow path to the overflow flow path, and the air in the overflow flow path and the connection passage is sucked from the air hole 29 provided in the overflow chamber by the suction pump. Due to the pressure difference that can be generated, the solution component existing in the connection passage is preferentially transferred to the overflow channel connecting portion with the overflow chamber, so that the solution component existing in the connection passage can be discharged, and the holding flow By adjusting the opening area of the channel and overflow channel, the allowable volume held by each channel can be determined, and the solid component after centrifugation or the high-concentration solid component solution can be transferred by the required amount. And improve the measurement accuracy of the analytical device. There is an effect that can.

(Embodiment 3)
Hereinafter, the analysis device 301 according to the third embodiment corresponding to claim 6, claim 7, claim 9 and claim 10 of the present invention and a measuring apparatus using the same will be described with reference to FIGS. I will explain.

  Note that the main configuration of the analysis device 301 according to the third embodiment and the analysis apparatus to which the analysis device is attached are the same as those described in the first embodiment, and thus description thereof is omitted here. .

  FIG. 9 is a plan view showing a microchannel configuration of the analyzing device 301 according to the third embodiment. 10 (a) and 10 (b) are diagrams for explaining the injection / separation process of the analyzing device 301 in the third embodiment, and FIGS. 11 (a) and 11 (b) are diagrams in the third embodiment. It is a figure for demonstrating the measurement process of the device 301 for an analysis, and the filling process of the measurement cell 28. FIG.

  As shown in FIG. 9, the microchannel configuration of the analysis device 301 according to the third embodiment has a liquid storage chamber 9 for injecting / accommodating a sample solution in an amount necessary for analysis, and a sample solution. A separation chamber 10 for separating the solution component and the solid component using a centrifugal force generated by the rotation of the analytical device 301, and a part of the solution component separated in the separation chamber 10 are transferred, A holding channel 13 for holding this, a sample overflow chamber 17 for discharging the sample solution remaining in the separation chamber 10, a connecting passage 14 connecting the separation chamber 10 and the sample overflow chamber 17, and holding It comprises a measurement cell 28 for holding the solution component filled in the flow path 13, mixing / reacting the solution component and the reagent, and measuring the absorbance and turbidity of the mixed solution.

  Here, in the third embodiment, a reagent for reacting with a solution component is carried in the measurement cell 28. Although omitted in the third embodiment, a reagent reaction chamber for reacting the sample solution with the reagent, a stirring chamber for stirring, and the like are provided between the holding channel 13 and the measurement cell 28. It doesn't matter.

  In the third embodiment, the liquid storage chamber 9, the separation chamber 10, the sample overflow chamber 17, and the measurement cell 28 are formed with a depth of 0.3 mm to 2 mm. It can be adjusted according to conditions for measurement (optical path length, measurement wavelength, reaction concentration of sample solution, type of reagent, etc.).

  The liquid storage chamber 9 is connected to the separation chamber 10, and as shown in FIG. 10A, a pre-weighed amount of sample solution is injected / stored from the injection port 8, and the analysis device 301 is rotated. By generating the centrifugal force, the sample solution can be transferred to the separation chamber 10 as shown in FIG.

  In the third embodiment, the separation chamber 10 is not provided with a measuring function for holding a constant amount of the sample solution. However, in order to reduce the steps before the sample solution injection, the separation chamber 10 is used for measuring the sample solution. Metering function, for example, a configuration that allows excess liquid to flow from the liquid level position where the required amount of the separation chamber can be maintained to the overflow chamber via the overflow channel when the analytical device is rotated, or from the separation chamber A capillary channel formed in communication with the outside of the analytical device is provided, the sample solution is aspirated by the capillary force of the capillary channel, the sample solution is weighed by the volume of the capillary channel, and the capillary flow by the centrifugal force A configuration for transferring the sample solution in the channel to the separation chamber may be provided.

  In the third embodiment, the liquid storage chamber 9 and the separation chamber 10 are connected with the same depth, but in order to prevent the sample solution from flowing into the separation chamber 10 during injection, air holes are provided in the separation chamber 10. It may be provided and connected by a capillary channel having a depth of 50 μm to 200 μm.

  The separation chamber 10 is connected to the holding channel 13 via the connection passage 11 from the position where the solution component of the separated sample solution is present, and further to the outer position than the radially outermost position of the separation chamber 10. The sample overflow chamber 17 is connected via a connection passage 14 having a siphon shape.

  The connecting passage 11 and the connecting passage 14 are formed with a passage width of 0.5 mm to 2 mm and a depth of 50 μm to 200 μm. This is due to the capillary force generated when the analysis device stops rotating and the connecting passage 11 and The connection passage 14 is not particularly limited as long as it can be filled with the sample solution.

  In the configuration of the third embodiment, when the analysis device 301 is rotated and transferred from the liquid storage chamber 9 to the separation chamber 10, the connection position of the connection channel 11 and the holding channel 13, and the connection channel 14. From the amount of the sample solution measured in advance so that the sample solution does not flow out of the separation chamber 10 beyond the inflection point of the siphon, the size of the separation chamber 10, the connection position of the connection channel 11 and the holding channel 13, It is necessary to optimize the position of the bending point of the siphon in the connection passage 14 to prevent the sample solution from flowing out. For this reason, in the third embodiment, the connection passage 11 is formed from the liquid surface when the separation chamber 10 holds a certain amount of sample solution to the inner peripheral position, and the connection passage 14 is also formed in the separation chamber 10. Forms a bending point of the siphon at an inner peripheral position with respect to the liquid surface at the time of holding a certain amount of sample solution, and as a result, as shown in FIG. The transferred sample solution is held in the separation chamber 10, the connection passage 11, and the connection passage 14.

  The sample solution held in the separation chamber 10 can be separated into a solution component 31 and a solid component 32 as shown in FIG. 10B by rotating at a high speed for several minutes. For example, in the case of blood, the rotation speed is set to 4000 rpm to 6000 rpm, and by rotating for 1 minute to 5 minutes, it can be separated into plasma and blood cells or high hematocrit blood (blood with a high ratio of blood cell components). it can.

  The separation chamber 10 is connected to the holding flow path 13 via the connection passage 11, and the holding flow path 13 is connected to the measurement cell 28 in the outer peripheral direction with respect to the holding flow path 13.

  The depth of the holding channel 13 is 50 μm to 200 μm, and when the analysis device 301 stops rotating, the holding channel 13 is filled with the solution component separated in the separation chamber 10 by capillary force. To do.

  Since the holding channel 13 can measure the solution according to the volume of the channel, the permissible volume of the solution component 31 is determined by adjusting the opening area of the holding channel 13 having a certain depth. It is possible to maintain the necessary liquid amount.

  The liquid filled in the holding channel 13 is connected by rotating the analysis device and applying centrifugal force, so that air is mixed from the air holes 18 and pressure is applied to the boundary between the holding channel 13 and the connecting channel 11. The liquid that has been broken is broken at the position of the air hole 18, that is, at the boundary between the holding flow path 13 and the connection passage 11, and the liquid filled between the air hole 18 and the air hole 19 flows into the measurement cell 28.

  After the holding channel 13 is filled in the state as shown in FIG. 11A, the solution component held in the holding channel 13 by rotating the analytical device again is shown in FIG. 11B. As shown, it is transferred to the measurement cell 28 by centrifugal force.

  Here, in Embodiment 3, when the sample solution remaining in the separation chamber 10 has transferred the liquid in the holding channel 13 to the measurement cell 28 and then stopped the rotation of the analysis device, In order to prevent the mixing ratio of the liquid in the measuring cell 28 from changing again by flowing into the holding channel 13 by the capillary force and into the measuring cell 28 at the next rotation, the separation ratio is reduced by the siphon effect of the connecting passage 14. The sample solution in the chamber 10 is discharged to the sample overflow chamber 17.

  The solution component that has flowed into the measurement cell 28 is mixed with the reagent carried in the measurement cell 28 by the acceleration / deceleration of rotation or the diffusion of the liquid while the rotation is stopped, but an external force such as vibration is used. It is also possible to mix them.

  The component to be analyzed contained in the solution component mixed with the reagent in the measurement cell 28 can calculate the concentration in the sample solution by measuring the reaction state with the reagent by absorbance measurement or the like. .

According to the analysis device 301 and the analysis apparatus using the same according to the third embodiment, the liquid storage chamber 9 for storing the sample solution and the liquid storage chamber communicate with the liquid by capillary force. A sample solution is transferred from the chamber, and a holding channel 13 for holding a part of the sample solution and a sample solution in the holding channel transferred by the centrifugal force generated by the rotation of the analytical device are held. When the measurement cell 28 and the analysis device are rotated about the axis, the measurement cell 28 and the liquid storage chamber are connected to the liquid storage chamber via the connection passage 14 that is located outside the axis and has a siphon structure. Since the sample overflow chamber 17 is provided, the liquid component after centrifugation can be transferred by a necessary amount. Further, when a part of the sample solution is transferred, it is possible to prevent the remaining solution from flowing in later, and the effect of improving the measurement accuracy of the analytical device can be obtained.

(Embodiment 4)
Hereinafter, an analysis device 401 according to a fourth embodiment corresponding to claims 6, 8, 9, and 10 of the present invention and a measuring apparatus using the same will be described with reference to FIGS. I will explain.

  Note that the main configuration of the analysis device 401 according to the fourth embodiment and the analysis apparatus to which the analysis device is attached are the same as those described in the first embodiment. Omitted.

  FIG. 12 is a plan view showing the microchannel configuration of the analysis device 401 according to the fourth embodiment. FIGS. 13A and 13B are diagrams for explaining the injection / separation process of the analysis device 401 in the fourth embodiment, and FIGS. 14A and 14B are diagrams in the fourth embodiment. It is a figure for demonstrating the measurement process of the device for analysis 401, and a measurement cell filling process.

  As shown in FIG. 12, the microchannel configuration of the analysis device 401 according to the fourth embodiment includes a liquid storage chamber 9 for injecting / accommodating a sample solution in an amount necessary for analysis, and a sample solution. A separation chamber 10 for separating the solution component and the solid component using a centrifugal force generated by the rotation of the analytical device, and a part of the solution component separated in the separation chamber 10 are transferred and retained. A holding channel 13, a sample overflow chamber 17 for discharging the sample solution remaining in the separation chamber 10, a connecting passage 14 connecting the separation chamber 10 and the sample overflow chamber 17, and the holding channel 13. It comprises a measurement cell 28 for holding a solution component filled therein, mixing / reacting the solution component and a reagent, and measuring the absorbance and turbidity of the mixed solution.

  Here, in this Embodiment 4, the reagent for making it react with a solution component is carry | supported in the measurement cell. Although omitted in the fourth embodiment, a reagent reaction chamber for reacting a sample solution with a reagent, a stirring chamber for stirring, and the like are provided between the holding channel 13 and the measurement cell 28. Also good.

  In the fourth embodiment, the liquid storage chamber 9, the separation chamber 10, the sample overflow chamber 17, and the measurement cell 28 are formed with a depth of 0.3 mm to 2 mm. However, in order to measure the amount and absorbance of the sample solution. Can be adjusted according to the conditions (optical path length, measurement wavelength, reaction concentration of sample solution, type of reagent, etc.).

  The liquid storage chamber 9 is connected to the separation chamber 10, and as shown in FIG. 13 (a), a pre-weighed amount of sample solution is injected / stored from the injection port 8, and the analytical device is rotated to rotate the centrifugal force. By generating the sample solution, the sample solution can be transferred to the separation chamber 10 as shown in FIG.

  In the fourth embodiment, the separation chamber 10 is not provided with a measuring function for holding a constant amount of the sample solution. However, in order to reduce the steps before the sample solution injection, the separation chamber 10 is used for measuring the sample solution. Metering function, for example, a configuration that allows excess liquid to flow from the liquid level position where the required amount of the separation chamber can be maintained to the overflow chamber via the overflow channel when the analytical device is rotated, or from the separation chamber A capillary channel formed in communication with the outside of the analytical device is provided, the sample solution is aspirated by the capillary force of the capillary channel, the sample solution is weighed by the volume of the capillary channel, and the capillary flow by the centrifugal force A configuration for transferring the sample solution in the channel to the separation chamber may be provided.

  In the fourth embodiment, the liquid storage chamber 9 and the separation chamber 10 are connected with the same depth, but in order to prevent the sample solution from flowing into the separation chamber 10 during injection, air holes are provided in the separation chamber 10. It may be provided and connected by a capillary channel having a depth of 50 μm to 200 μm.

  The separation chamber 10 is connected to the holding channel 13 via the connection passage 11 from the position where the solution component of the separated sample solution is present, and further to the outer position than the radially outermost position of the separation chamber 10. The sample overflow chamber 17 is connected via a connection passage 14 having a siphon shape.

  The connecting passage 11 and the connecting passage 14 are formed with a passage width of 0.5 mm to 2 mm and a depth of 50 μm to 200 μm. However, the connecting passage 11 and the connecting passage 14 are generated by the capillary force generated when the analysis device stops rotating. There is no particular limitation as long as the inside can be filled with the sample solution.

  When the analysis device 401 is rotated and transferred from the liquid storage chamber 9 to the separation chamber 10, the connection position of the connection passage 11 and the holding flow passage 13 and the bending point of the siphon of the connection passage 14 are exceeded. In order to prevent the sample solution from flowing out of the separation chamber 10, the size of the separation chamber 10, the connection position of the connection passage 11 and the holding flow path 13, and the siphon bending point position of the connection passage 14 are determined based on the amount of the sample solution weighed in advance. Etc. should be optimized to prevent the sample solution from flowing out. In the present invention, the connection passage 11 is formed from the liquid surface when the separation chamber 10 holds a certain amount of sample solution to the inner peripheral position, and the connection passage 14 also includes the separation chamber 10 having a certain amount of sample solution. Since the inflection point of the siphon is formed at the inner peripheral position with respect to the liquid level when holding the sample solution, the sample solution transferred from the liquid storage chamber 9 by the centrifugal force is separated from the separation chamber as shown in FIG. 10, the connecting passage 11, and the connecting passage 14.

  The sample solution held in the separation chamber 10 can be separated into a solution component 31 and a solid component 32 as shown in FIG. 13B by rotating at a high speed for several minutes. For example, in the case of blood, the rotation speed is set to 4000 rpm to 6000 rpm, and rotation is performed for 1 minute to 5 minutes, whereby plasma and blood cells or high hematocrit blood (blood with a high ratio of blood cell components) can be separated.

The separation chamber 10 is connected to the holding flow path 13 via the connection passage 11, and the holding flow path 13 is connected to the measurement cell 28 in the outer peripheral direction with respect to the holding flow path 13.

  The depth of the holding channel 13 is 50 μm to 200 μm, and when the rotation of the analysis device is stopped, the solution component separated in the separation chamber 10 is filled into the holding channel 13 by capillary force.

  Since the holding channel 13 can measure the solution according to the volume of the channel, the permissible volume of the solution component 31 is determined by adjusting the opening area of the holding channel 13 having a certain depth. It is possible to maintain a necessary liquid amount.

  The liquid filled in the holding channel 13 is connected to a gas introduction mechanism (not shown) in the air hole 18 in a state in which the rotation of the analyzing device is stopped, and the gas is introduced from the air hole 18 to hold the liquid. Pressure is applied to the boundary between the passage 13 and the connection passage 11, and the connected liquid is broken at the position of the air hole 18, that is, at the boundary between the holding passage 13 and the connection passage 11, and filled between the air hole 18 and the measurement cell 28. The liquid that has been flown into the measurement cell 28. Here, the gas introduction mechanism is composed of a compression pump, a gas generation source that can send out gas such as air and nitrogen such as a high-pressure gas cylinder, and a pipe for connecting the gas generation source and the analytical device. It can be detached from the air hole 18 of the analyzing device.

  After the holding flow path 13 is filled in the state shown in FIG. 14A, the solution components held in the holding flow path 13 by introducing the gas into the analysis device are shown in FIG. ), The gas is transferred to the measuring cell 28 by the pressure of the gas.

  Here, in the fourth embodiment, after the sample solution remaining in the separation chamber 10 transfers the liquid in the holding channel 13 to the measurement cell 28, the introduction of the gas into the analysis device is stopped. In order to prevent the mixture ratio of the liquid in the measurement cell 28 from being changed by flowing into the holding channel 13 again by capillary force and flowing into the measurement cell 28 again at the next rotation or gas introduction, The sample solution in the separation chamber 10 is discharged to the sample overflow chamber 17 by the siphon effect 14.

  The solution component that has flowed into the measurement cell 28 is mixed with the reagent carried in the measurement cell 28 by the acceleration / deceleration of rotation or the diffusion of the liquid while the rotation is stopped, but an external force such as vibration is used. It is also possible to mix them.

  The component to be analyzed contained in the solution component mixed with the reagent in the measurement cell 28 can calculate the concentration in the sample solution by measuring the reaction state with the reagent by absorbance measurement or the like. .

According to the analysis device 401 and the analysis apparatus using the analysis device according to the fourth embodiment, the liquid storage chamber 9 for storing the sample solution and the liquid storage chamber communicate with each other by the capillary force. Due to the pressure difference generated by introducing the gas from the holding channel 13 for transferring the sample solution from the chamber and holding a part of the sample solution and the air hole 18 provided at the liquid separation position of the holding channel. When the measurement cell 28 for holding the sample solution in the transported holding channel and the analysis device are rotated around the axis, the siphon structure is positioned outside the axis with respect to the liquid storage chamber. Since the sample overflow chamber 17 connected to the liquid storage chamber 9 via the connecting passage 14 is provided, the liquid component after centrifugation can be transferred by a necessary amount. In addition, when a part of the sample solution is transferred, it is possible to prevent the remaining solution from flowing in later, and the effect of improving the measurement accuracy of the analytical device can be obtained.

(Embodiment 5)
Hereinafter, an analysis device according to a fifth embodiment corresponding to claims 4, 11, and 12 of the present invention and a measurement apparatus using the same will be described with reference to FIGS.

  Note that the main configuration of the analysis device 501 of the fifth embodiment and the analysis apparatus to which the analysis device is attached are the same as those in the first embodiment, and a description thereof is omitted here.

  FIG. 15 is a plan view showing a microchannel configuration of the analyzing device 501 in the fifth embodiment. FIGS. 16A and 16B are diagrams for explaining the injection / separation process of the analyzing device 501 in the fifth embodiment, and FIGS. 17A and 17B are diagrams in the fifth embodiment. FIGS. 18A, 18B, and 18C are diagrams for explaining the weighing process of analysis device 501. FIGS. 18A, 18B, and 18C are diagrams for explaining the mixing / measurement cell filling process of analysis device 501 in the fifth embodiment. FIG.

  As shown in FIG. 15, the microchannel configuration of the analysis device 501 of the fifth embodiment includes a liquid storage chamber 9 for injecting / accommodating an amount of sample solution necessary for analysis, and a sample solution. A separation chamber 10 for separating the solution component and the solid component using centrifugal force generated by the rotation of the analytical device, and a part of the solid component separated in the separation chamber 10 are transferred and retained. A holding channel 13 for overflowing, an overflow channel 12 connected by a connecting channel 11 for transferring the sample solution in the separation chamber 10 between the holding channel 13 and the separation chamber 10, and an overflow channel The overflow chamber 15 from which the sample solution filled in 12 is discharged, the sample overflow chamber 17 for discharging the sample solution remaining in the separation chamber 10, and the separation chamber 10 and the sample overflow chamber 17 are connected. Connecting passage 14 A diluent storage chamber 22 for injecting / accommodating a diluent containing a denaturant for diluting or reacting a solid component with a specific reagent / antibody; a measuring chamber 23 for holding a fixed amount of the diluent; A mixing chamber 16 for mixing / stirring the solid component and the diluent from the holding channel 13, and holding the mixed liquid, and measuring the absorbance, turbidity, or number of cells of the mixed liquid. Measurement cell 28.

  Here, although omitted in the fifth embodiment, a reagent reaction chamber for reacting the sample solution with the reagent, a stirring chamber for stirring, and the like are provided between the mixing chamber 16 and the measurement cell 28. It doesn't matter.

  In the fifth embodiment, the liquid storage chamber 9, the separation chamber 10, the sample overflow chamber 17, the overflow chamber 15, the diluent storage chamber 22, the measuring chamber 23, the diluent overflow chamber 24, the mixing chamber 16, and the measurement cell. The depth of 28 is 0.3 mm to 2 mm, but it can be adjusted according to the amount of sample solution and the conditions for measuring absorbance (optical path length, measurement wavelength, sample solution reaction concentration, reagent type, etc.). is there.

  Further, the liquid storage chamber 9 is connected to the separation chamber 10, and as shown in FIG. 16A, a pre-weighed amount of sample solution is injected / stored from the injection port 8, and the analysis device is rotated. By generating the centrifugal force, the sample solution can be transferred to the separation chamber 10 as shown in FIG.

  In the fifth embodiment, the separation chamber 10 is not provided with a measuring function for holding a constant amount of the sample solution. However, in order to reduce the steps before the sample solution is injected, the separation chamber 10 is used for measuring the sample solution. Metering function, for example, a configuration that allows excess liquid to flow from the liquid level position where the required amount of the separation chamber can be maintained to the overflow chamber via the overflow channel when the analytical device is rotated, or from the separation chamber A capillary channel formed in communication with the outside of the analytical device is provided, the sample solution is aspirated by the capillary force of the capillary channel, the sample solution is weighed by the volume of the capillary channel, and the capillary flow by the centrifugal force A configuration for transferring the sample solution in the channel to the separation chamber may be provided.

  In the fifth embodiment, the liquid storage chamber 9 and the separation chamber 10 are connected at the same depth, but in order to prevent the sample solution from flowing into the separation chamber 10 during injection, air is supplied to the separation chamber 10. A hole may be provided and connected by a capillary channel having a depth of 50 μm to 200 μm.

  The separation chamber 10 is connected to the overflow channel 12 from the radially outermost position of the separation chamber 10 via the connecting passage 11 and is further outward from the radially outermost position of the separation chamber 10. The sample overflow chamber 17 is connected via a connection passage 14 having a siphon shape.

  In addition, the connecting passage 11 and the connecting passage 14 are formed with a passage width of 0.5 mm to 2 mm and a depth of 50 μm to 200 μm. This is a capillary force generated when rotation of the analytical device is stopped. The connection passage 11 and the connection passage 14 are not particularly limited as long as they can be filled with the sample solution.

In the configuration of the fifth embodiment, when the analysis device 501 is rotated and transferred from the liquid storage chamber 9 to the separation chamber 10, the connection position of the connection passage 11 and the overflow channel 12, and the connection passage In order to prevent the sample solution from flowing out of the separation chamber 10 beyond the inflection point of 14 siphons, the size of the separation chamber 10 and the connection between the connection passage 11 and the overflow flow passage 12 are determined based on the amount of the sample solution weighed in advance. It is necessary to prevent the sample solution from flowing out by optimizing the position, the position of the inflection point of the siphon in the connection passage 14, and the like. For this reason, in the fifth embodiment, the connection passage 11 is formed from the liquid surface when the separation chamber 10 holds a certain amount of sample solution to the inner peripheral position, and the connection passage 14 is also formed in the separation chamber 10. because There is formed a bending point of the siphon on the inner circumferential position than the liquid surface when holding an quantitation of the sample solution, as shown in FIG. 16 (b), is transferred from the liquid storage chamber 9 by the centrifugal force The sample solution is held in the separation chamber 10, the connecting passage 11, and the connecting passage 14.

  The sample solution held in the separation chamber 10 can be separated into a solution component 31 and a solid component 32 as shown in FIG. 16B by rotating at a high speed for several minutes. For example, in the case of blood, the rotation speed is set to 4000 rpm to 6000 rpm, and rotation is performed for 1 minute to 5 minutes, whereby plasma and blood cells or high hematocrit blood (blood with a high ratio of blood cell components) can be separated.

  The separation chamber 10 is connected to the holding channel 13 via the connecting passage 11 and the overflow channel 12, and the overflow channel 12 is connected to the overflow chamber 15 located in the outer peripheral direction with respect to the overflow channel 12. The holding flow path 13 is connected to the mixing chamber 16 located in the outer peripheral direction with respect to the holding flow path 13.

  The depths of the holding channel 13 and the overflow channel 12 are 50 μm to 200 μm, and when the analysis device stops rotating, the separated solid component or high concentration in the separation chamber 10 is generated by capillary force. The solid component solution is filled into the holding channel 13 and the overflow channel 12.

  At that time, all of the solution components 31 separated in the connection passage 11 are first transferred to the overflow channel 12 by capillary force, but the overflow chamber 15 is formed deep. The solution component 31 is not transferred into the overflow chamber 15 but is held in the coupling portion 30 between the overflow channel 12 and the overflow chamber 15. Thereafter, the solid component 32 separated in the connection passage 11 and the separation chamber 10 is transferred to the holding passage 13 via the overflow passage 12.

  In the fifth embodiment, as shown in FIG. 16 (b), when the sample solution is centrifuged in the separation chamber 10, the inner peripheral portion of the connecting passage 11 connecting the overflow channel 12 and the separation chamber 10 is Since the solution component 31 is present, if it flows into the holding channel 13 as it is, the concentration of the solid component is lowered, resulting in a variation in measurement accuracy. Therefore, as shown in FIG. By making the opening area of the overflow channel 12 at the junction 30 between the overflow channel 12 and the overflow chamber 15 larger than the opening area of the retention channel 13 at the branch point of the holding channel 13, the solution component 31. Preferentially flows into the overflow channel 12. Here, the ratio of the opening area of the overflow channel 12 to the holding channel 13 is preferably 1.5 to 5 times. If it is 1.5 times or less, the solution component 31 may flow into the holding channel 13. If it is 5 times or more, the area of the overflow channel 12 becomes too large, so the solid component 32 is filled more than necessary. As a result, the loss of the solid component 32 may increase.

  In the fifth embodiment, the overflow channel 12 is provided between the connection channel 11 and the holding channel 13, but the opening area at the branch point between the holding channel 13 and the overflow channel 12 is defined as the present embodiment. In the same manner as described above, by making the opening area of the overflow channel 12 larger than the opening area of the holding channel 13, the solution component 31 can be preferentially caused to flow into the overflow channel 12. It is also possible to provide the overflow channel 12 via the holding channel 13.

  Since the holding flow path 13 and the overflow flow path 12 can measure the solution according to the volume of the flow path, the solid component 32 is an opening of the holding flow path 13 and the overflow flow path 12 having a certain depth. By adjusting the area, the allowable volume is determined, and the necessary liquid amount can be maintained. The allowable volume of the overflow channel 12 is designed so that all the solution components 31 existing in the connecting passage 11 can be accommodated, and at least 0.5 times the amount of the solid component 32 that is larger than the accommodated solution component 31 can flow. By doing so, it is possible to suppress the solution component 31 from flowing into the holding channel 13.

  The liquid filled in the holding channel 13 rotates the device for analysis and applies centrifugal force, so that air is mixed from the air holes 19 and pressure is applied to the boundary between the holding channel 13 and the overflow channel 12. The connected liquid is broken at the position of the air hole 19, that is, at the boundary between the holding flow path 13 and the overflow flow path 12, and the liquid filled between the air hole 19 and the air hole 20 flows into the mixing chamber 16. To do.

Similarly, the liquid filled in the overflow channel 12 is also broken at the position of the air hole 18 the air hole 19, overflow chamber 15 is a liquid that has been filled between the air hole 19 from the position of the air hole 18 The liquid that has flowed into and separated from the position of the air hole 18 into the separation chamber 10 is returned into the separation chamber 10 by centrifugal force.

  After the holding channel 13 is filled as shown in FIG. 17B, the solid component held in the holding channel 13 by rotating the analytical device again is shown in FIG. 18A. Then, it is transferred to the mixing chamber 16 by centrifugal force.

  Here, in the present fifth embodiment, when the sample solution remaining in the separation chamber 10 transfers the liquid in the holding channel 13 to the mixing chamber 16 and then stops the rotation of the analysis device, In order to prevent the mixing ratio of the liquid in the mixing chamber 16 from changing again by flowing into the holding flow path 13 by the capillary force and flowing again into the mixing chamber 16 at the next rotation, the separation by the siphon effect of the connecting passage 14 The sample solution in the chamber 10 is discharged to the sample overflow chamber 17.

  The diluent storage chamber 22 is connected to the measuring chamber 23. As shown in FIG. 16 (a), the diluent is injected / stored from the injection port 21, and the analysis device is rotated, so that FIG. As shown in FIG. 3, the diluent can be transferred to the measuring chamber 23.

  In the fifth embodiment, the diluent storage chamber 22 and the measuring chamber 23 are connected with the same depth, but the depth is set to prevent the diluent from flowing into the measuring chamber 23 when the diluent is injected. By connecting the diluent storage chamber 22 and the measuring chamber 23 with a capillary channel of 50 μm to 200 μm, it is possible to suppress the inflow of the diluent.

  The measuring chamber 23 is overflowed at the inlet of the diluent overflow chamber 24 arranged radially inward of the measuring chamber 23 and located radially inward of the measuring chamber 23 adjacent to the diluent overflow chamber 24. It is connected from the mouth via a capillary channel 25 and connected to the mixing chamber 16 via a connecting passage 26 from a location located radially outward of the measuring chamber 23. The dilution liquid overflow chamber 24 is provided with air holes so that the dilution liquid can easily flow in, and the mixing chamber 16 is also provided with air holes so that the dilution liquid can easily flow through the connecting passage 26.

  The connecting passage 26 has a siphon shape including a curved tube disposed inward from the distance from the rotation center of the analyzing device to the inlet of the diluent overflow chamber 24 and the interface of the capillary channel 25. In the present invention, the capillary channel 25 and the connecting passage 26 are formed with a width of 0.5 mm to 2 mm and a depth of 50 μm to 200 μm. However, if the inside of the connecting passage 26 can be filled with a diluent by capillary force. There is no particular limitation.

  By connecting the measuring chamber 23 and the mixing chamber 16 in this way, the diluent stored in the diluent storing chamber 22 can be transferred and filled into the measuring chamber 23 by the rotation of the analyzing device. ), The diluent in the connection passage 26 reaches a position corresponding to the radial distance from the center of rotation of the analytical device to the inlet of the diluent overflow chamber 24 and the interface of the capillary channel 25. Only filled. When the analysis device is stopped after the filling of the measuring chamber 23 is completed, a capillary force acts in the connection passage 26 and the inlet of the mixing chamber 16 is filled with the diluent as shown in FIG. At this time, the diluent does not flow into the mixing chamber 16 because the mixing chamber 16 is deep and the capillary force is extremely small compared to the capillary force of the connecting passage 26.

  After the holding channel 13 and the connection channel 26 are filled in the state as shown in FIG. 17B, the analysis component is rotated again to thereby hold the solid component held in the holding channel 13 and the measuring chamber As shown in FIG. 18A, the diluted solution held in 23 is transferred to the mixing chamber 16 by centrifugal force and siphon effect, and the sample solution filled in the overflow channel 12 is overflow chamber. 15, the sample solution held in the separation chamber 10 is transferred to the sample overflow chamber 17.

  Since the solid component and the diluting liquid that have flowed into the mixing chamber 16 are connected to the measurement cell 28 via the connection passage 27 having a siphon shape, the solid component that is held in the mixing chamber 16 and the diluted solid component is rotated. It is mixed by acceleration / deceleration or diffusion of liquid while rotation is stopped.

  The connecting passage 27 is formed with a passage width of 0.5 mm to 2 mm and a depth of 50 μm to 200 μm, but there is no particular limitation as long as the inside of the connecting passage 27 can be filled with a liquid by capillary force.

  As shown in FIG. 18 (b), as shown in FIG. 18 (b), the mixed liquid is filled with the mixed liquid 27 with rotation of the mixed passage 27 and rotated again as shown in FIG. 18 (b). The liquid in the mixing chamber 16 can be transferred to the measurement cell 28.

  If the sample solution is blood, the dilute solution and blood cell components are mixed in the mixing chamber 16, whereby blood cells are destroyed and hemolyzed, and hemoglobin in the blood cells is mixed with the dilute solution. By measuring the absorbance of the diluted hemoglobin in a measurement cell, the hemoglobin concentration in the blood can be calculated.

  According to the analysis device 501 and the analysis apparatus using the same according to the fifth embodiment, in the analysis device according to the first embodiment, the solid component and the dilute solution or reagent communicate with the holding channel. Since the mixing chamber 16 for mixing the solution and the diluting solution storage chamber 22 communicating with the mixing chamber and containing the diluting solution or the reagent solution are further provided, the same as in the first embodiment Depending on the process, the solid component after centrifugation or high-concentration solid component solution can be transferred to the holding channel by the required amount, and the solid component transferred to the holding channel can be mixed with the dilute solution or reagent solution. Then, it can be transferred to the measuring cell 28. In addition, when a part of the sample solution is transferred, it is possible to prevent the remaining solution from flowing into the holding channel after the follow-up, and the measurement accuracy of the analytical device can be improved.

(Embodiment 6)
Hereinafter, an analysis device 601 according to a sixth embodiment corresponding to claims 4, 11, 12, and 13 of the present invention, and a measuring apparatus using the same will be described with reference to FIGS. I will explain.

  Note that the main configuration of the analysis device 601 according to the sixth embodiment and the analysis apparatus to which the analysis device is attached are the same as those in the first embodiment, and a description thereof is omitted here.

  FIG. 19 is a plan view showing the microchannel configuration of the analysis device 601 of the sixth embodiment. 20A and 20B are diagrams for explaining the injection / separation process of the analysis device 601 according to the sixth embodiment, and FIGS. 21A and 21B are diagrams according to the sixth embodiment. FIGS. 22A, 22B, and 22C are diagrams for explaining the weighing process of the analysis device 601. FIGS. 22A, 22B, and 22C are diagrams for explaining the mixing / measurement cell filling process of the analysis device 601 in the sixth embodiment. FIG.

  As shown in FIG. 19, the microchannel configuration of the analysis device 601 of the sixth embodiment includes a liquid storage chamber 9 for injecting / accommodating a sample solution in an amount necessary for analysis, and a sample solution. A separation chamber 10 for separating the solution component and the solid component from each other using a centrifugal force generated by the rotation of the analytical device, and a part of the solution component separated in the separation chamber 10 are connected to the separation chamber 10. The solution component holding flow path 36 that is transferred through the connecting passage 34 and holds the solution component, the solution component filled in the solution component holding flow path 36 is held, and the solution component and the reagent are mixed / reacted. A measurement cell 37 for measuring the absorbance and turbidity of the mixed liquid, and a holding channel 13 for transferring a part of the solid component separated in the separation chamber 10 and holding it. , Holding channel 13 and separation chamber Between 0 and the overflow channel 12 connected by the connecting passage 11 for transferring the sample solution in the separation chamber 10, and the overflow chamber 15 from which the sample solution filled in the overflow channel 12 is discharged. A sample overflow chamber 17 for discharging the sample solution remaining in the separation chamber 10, a connecting passage 14 connecting the separation chamber 10 and the sample overflow chamber 17, and a solid component diluted or a specific reagent / antibody A diluent storage chamber 22 for injecting / accommodating a diluent containing a modifying agent for reaction, a measuring chamber 23 for holding a fixed amount of the diluent, and for mixing / stirring the solid component and the diluent The mixing chamber 16 is constituted by a measurement cell 28 for holding the mixed liquid and measuring the absorbance, turbidity, or number of cells of the mixed liquid.

Here, in the sixth embodiment, a reagent for reacting with a solution component is carried in the measurement cell. Although omitted in this embodiment, between the mixing chamber 16 and the measurement cell 28 and between the solution component holding channel 36 and the measurement cell 37, a reagent reaction chamber for reacting a sample solution with a reagent, A stirring chamber or the like for stirring may be provided.

  In the sixth embodiment, the liquid storage chamber 9, the separation chamber 10, the sample overflow chamber 17, the overflow chamber 15, the diluent storage chamber 22, the measuring chamber 23, the diluent overflow chamber 24, the mixing chamber 16, and the measurement cell. 28, the measurement cell 37 is formed with a depth of 0.3 mm to 2 mm, but the conditions for measuring the amount and absorbance of the sample solution (optical path length, measurement wavelength, reaction concentration of the sample solution, type of reagent, etc.) Can be adjusted by.

  The liquid storage chamber 9 is connected to the separation chamber 10, and as shown in FIG. 20 (a), a pre-weighed amount of sample solution is injected / stored from the injection port 8, and the analytical device is rotated to rotate the centrifugal force. By generating the sample solution, the sample solution can be transferred to the separation chamber 10 as shown in FIG.

  In the sixth embodiment, the separation chamber 10 is not provided with a weighing function for holding a constant amount of the sample solution. However, in order to reduce the steps before the sample solution injection, the separation chamber 10 is used for weighing the sample solution. Metering function, for example, a configuration that allows excess liquid to flow from the liquid level position where the required amount of the separation chamber can be maintained to the overflow chamber via the overflow channel when the analytical device is rotated, or from the separation chamber A capillary channel formed in communication with the outside of the analytical device is provided, the sample solution is aspirated by the capillary force of the capillary channel, the sample solution is measured by the volume of the capillary channel, and the capillary flow by the centrifugal force A configuration for transferring the sample solution in the channel to the separation chamber may be provided.

  In the sixth embodiment, the liquid storage chamber 9 and the separation chamber 10 are connected with the same depth, but in order to prevent the sample solution from flowing into the separation chamber 10 during injection, air holes are provided in the separation chamber 10. It may be provided and connected by a capillary channel having a depth of 50 μm to 200 μm.

The separation chamber 10 is connected to the solution component holding channel 36 via the connection passage 34 from the position where the solution component of the separated sample solution exists, and is connected to the connection passage from the radially outermost position of the separation chamber 10. 11 is connected to the overflow channel 12 through the connection channel 14 and further from the radially outermost position of the separation chamber 10 to the outer position through a connection passage 14 having a siphon shape. Yes.

  The connection passage 11, the connection passage 34, and the connection passage 14 are formed with a passage width of 0.5 mm to 2 mm and a depth of 50 μm to 200 μm, but the connection passage is caused by the capillary force generated when the analysis device stops rotating. 11, the connection passage 34, and the connection passage 14 are not particularly limited as long as they can be filled with the sample solution.

In the configuration of the sixth embodiment, when the analysis device 601 is rotated and transferred from the liquid storage chamber 9 to the separation chamber 10, the connection position of the connection channel 11 and the overflow channel 12, the connection channel 34. From the amount of the sample solution weighed in advance so that the sample solution does not flow out of the separation chamber 10 beyond the connection position of the solution component holding channel 36 and the siphon bending point of the connection passage 14. By optimizing the size, the connecting position of the connecting passage 11 and the overflow channel 12, the connecting position of the connecting passage 34 and the solution component holding channel 36, the siphon bending point position of the connecting passage 14 and the like, the outflow of the sample solution is optimized. It is necessary to prevent. For this reason, in the sixth embodiment, the connection passage 11 and the connection passage 34 are formed from the liquid surface when the separation chamber 10 holds a certain amount of sample solution to the inner peripheral position. 14 also forms an inflection point of the siphon at an inner peripheral position from the liquid surface when the separation chamber 10 holds a certain amount of the sample solution. As a result, as shown in FIG. The sample solution transferred from the liquid storage chamber 9 is held in the separation chamber 10, the connection passage 11, the connection passage 34, and the connection passage 14.

  The sample solution held in the separation chamber 10 can be separated into a solution component 31 and a solid component 32 as shown in FIG. 20B by rotating at a high speed for several minutes. For example, in the case of blood, the rotation speed is set to 4000 rpm to 6000 rpm, and by rotating for 1 minute to 5 minutes, it can be separated into plasma and blood cells or high hematocrit blood (blood with a high ratio of blood cell components). it can.

The separation chamber 10 is connected to the holding channel 13 via the connection passage 11 and the overflow channel 12, and is connected to the solution component holding channel 36 via the connection channel 34. The channel 12 is connected to the overflow chamber 15 in the outer circumferential direction with respect to the overflow channel 12, the holding channel 13 is connected to the mixing chamber 16 in the outer circumferential direction with respect to the holding channel 13, and the solution component holding channel 36. Is connected to a measurement cell 37 located in the outer peripheral direction with respect to the solution component holding channel 36.

  The depths of the holding channel 13 and the overflow channel 12 are 50 μm to 200 μm, and when the analysis device stops rotating, the separated solid component or high concentration in the separation chamber 10 is generated by capillary force. The solid component solution is filled into the holding channel 13 and the overflow channel 12.

  At that time, all of the solution components 31 separated in the connection passage 11 are first transferred to the overflow channel 12 by capillary force, but the overflow chamber 15 is formed deep. The solution component 31 is not transferred into the overflow chamber 15 but is held in the coupling portion 30 between the overflow channel 12 and the overflow chamber 15. Thereafter, the solid component 32 separated in the connection passage 11 and the separation chamber 10 is transferred to the holding passage 13 via the overflow passage 12.

  In the sixth embodiment, as shown in FIG. 20B, when the sample solution is centrifuged in the separation chamber 10, the inner peripheral portion of the connecting passage 11 connecting the overflow channel 12 and the separation chamber 10 is Since the solution component 31 is present, if it flows into the holding channel 13 as it is, the concentration of the solid component is reduced, resulting in a variation in measurement accuracy. Therefore, as shown in FIG. By making the opening area of the overflow channel 12 at the junction 30 between the overflow channel 12 and the overflow chamber 15 larger than the opening area of the retention channel 13 at the branch point of the holding channel 13, the solution component 31. Preferentially flows into the overflow channel 12. Here, the ratio of the opening area of the overflow channel 12 to the holding channel 13 is preferably 1.5 to 5 times. If it is 1.5 times or less, the solution component 31 may flow into the holding channel 13. If it is 5 times or more, the area of the overflow channel 12 becomes too large, so the solid component 32 is filled more than necessary. As a result, the loss of the solid component 32 may increase.

  In the sixth embodiment, the overflow channel 12 is provided between the connection channel 11 and the holding channel 13, but the opening area at the branch point of the holding channel 13 and the overflow channel 12 is defined as the present embodiment. In the same manner as described above, by making the opening area of the overflow channel 12 larger than the opening area of the holding channel 13, the solution component 31 can be preferentially caused to flow into the overflow channel 12. It is also possible to provide the overflow channel 12 via the holding channel 13.

  Since the holding flow path 13 and the overflow flow path 12 can measure the solution according to the volume of the flow path, the solid component 32 is an opening of the holding flow path 13 and the overflow flow path 12 having a certain depth. By adjusting the area, the allowable volume is determined, and the necessary liquid amount can be maintained. The allowable volume of the overflow channel 12 is designed so that all the solution components 31 existing in the connecting passage 11 can be accommodated, and at least 0.5 times the amount of the solid component 32 that is larger than the accommodated solution component 31 can flow. By doing so, it is possible to suppress the solution component 31 from flowing into the holding channel 13.

The depth of the solution component holding channel 36 is formed by 50 m to 200 m, when stopping the rotation of the analysis device, by capillary force, the separated solution component in the separation chamber 10 solution component holding channel 36 To fill.

Since the solution component holding channel 36 can measure the solution according to the volume of the channel, the solution component 31 has an allowable volume by adjusting the opening area of the solution component holding channel 36 having a certain depth. Is determined, and the necessary liquid volume can be maintained.

  The liquid filled in the holding channel 13 rotates the device for analysis and applies centrifugal force, so that air is mixed from the air holes 19 and pressure is applied to the boundary between the holding channel 13 and the overflow channel 12. The connected liquid is broken at the position of the air hole 19, that is, at the boundary between the holding flow path 13 and the overflow flow path 12, and the liquid filled between the air hole 19 and the air hole 20 flows into the mixing chamber 16. To do.

  Similarly, the liquid filled in the overflow channel 12 is also broken at the positions of the air hole 18 and the air hole 19, and the liquid filled between the position of the air hole 18 and the position of the air hole 20 is overflowed. The liquid that has flowed into 15 and filled between the separation chambers 10 from the position of the air holes 18 is returned into the separation chamber 10 by centrifugal force.

  Furthermore, the liquid filled in the solution component holding channel 36 is also broken at the position of the air hole 35, and the liquid filled between the measurement cells 37 from the position of the air hole 35 flows into the measurement cell 37, The liquid filled between the separation holes 10 from the position of the air hole 35 is returned into the separation chamber 10 by centrifugal force.

After the holding flow path 13 and the solution component holding flow path 36 are filled as shown in FIG. 21B, the solid component and the solution held in the holding flow path 13 by rotating the analysis device again. As shown in FIG. 21A, the solution components held in the component holding flow path 36 are respectively transferred to the mixing chamber 16 and the measurement cell 37 by centrifugal force.

Here, in the sixth embodiment, the sample solution remaining in the separation chamber 10 transfers the liquid in the holding channel 13 to the mixing chamber 16 and the liquid in the solution component holding channel 36. After the transfer to the measurement cell 37, when the rotation of the analyzing device is stopped, it flows into the holding channel 13 and the solution component holding channel 36 again by the capillary force, and at the next rotation, the mixing chamber 16 and the measuring cell 37 In order to prevent the mixing ratio of the liquid in the mixing chamber 16 and the measurement cell 37 from changing, the sample solution in the separation chamber 10 is discharged to the sample overflow chamber 17 by the siphon effect of the connecting passage 14. Yes.

The diluent storage chamber 22 is connected to the measuring chamber 23. As shown in FIG. 20A, the diluent is injected / stored from the injection port 21, and the analysis device is rotated, so that FIG. As shown in FIG. 3, the diluent can be transferred to the measuring chamber 23.

  In the sixth embodiment, the diluent storage chamber 22 and the measuring chamber 23 are connected with the same depth, but the depth is set to prevent the diluent from flowing into the measuring chamber 23 when the diluent is injected. By connecting the diluent storage chamber 22 and the measuring chamber 23 with a capillary channel of 50 μm to 200 μm, it is possible to suppress the inflow of the diluent.

  The measuring chamber 23 is overflowed at the inlet of the diluent overflow chamber 24 arranged radially inward of the measuring chamber 23 and located radially inward of the measuring chamber 23 adjacent to the diluent overflow chamber 24. It is connected from the mouth via a capillary channel 25 and connected to the mixing chamber 16 via a connecting passage 26 from a location located radially outward of the measuring chamber 23. The dilution liquid overflow chamber 24 is provided with air holes so that the dilution liquid can easily flow in, and the mixing chamber 16 is also provided with air holes so that the dilution liquid can easily flow through the connecting passage 26.

  The connecting passage 26 has a siphon shape including a curved tube disposed inward from the distance from the rotation center of the analyzing device to the inlet of the diluent overflow chamber 24 and the interface of the capillary channel 25. In the sixth embodiment, the capillary channel 25 and the connecting passage 26 are formed with a width of 0.5 mm to 2 mm and a depth of 50 μm to 200 μm. This is because the inside of the connecting channel 26 is diluted with a capillary force. If it can be filled with, there is no particular limitation.

  In this way, by connecting the measuring chamber 23 and the mixing chamber 16, even if the diluent stored in the diluent storage chamber 22 is transferred and filled into the measuring chamber 23 by the rotation of the analysis device, FIG. As shown in (b), the diluent in the connection passage 26 corresponds to the radial distance from the center of rotation of the analytical device to the inlet of the diluent overflow chamber 24 and the interface of the capillary channel 25. Fills only to the position. When the analysis device is stopped after the filling of the measuring chamber 23, a capillary force acts in the connection passage 26 and the inlet of the mixing chamber 16 is filled with the diluent as shown in FIG. At this time, the diluent does not flow into the mixing chamber 16 because the mixing chamber 16 is deep and the capillary force is extremely small compared to the capillary force of the connecting passage 26.

As shown in FIG. 21 (b), after the holding channel 13, the solution component holding channel 36, and the connection channel 26 are filled, the analytical device is rotated again to be held in the holding channel 13. The solid component, the solution component held in the solution component holding channel 36, and the diluent held in the measuring chamber 23 are mixed by centrifugal force and siphon effect, respectively, as shown in FIG. The sample solution transferred to the chamber 16 and the measurement cell 37 and filled in the overflow channel 12 is transferred to the overflow chamber 15, and the sample solution held in the separation chamber 10 is transferred to the sample overflow chamber 17. .

  The solution component that has flowed into the measurement cell 37 is mixed with the reagent carried in the measurement cell 37 by the acceleration / deceleration of rotation or the diffusion of the liquid while the rotation is stopped, but an external force such as vibration is used. It is also possible to mix them.

  The component to be analyzed contained in the solution component mixed with the reagent in the measurement cell 37 can calculate the concentration in the sample solution by measuring the reaction state with the reagent by absorbance measurement or the like. .

  Since the solid component and the diluting liquid that have flowed into the mixing chamber 16 are connected to the measurement cell 28 via the connection passage 27 having a siphon shape, the solid component that is held in the mixing chamber 16 and the diluted solid component is rotated. It is mixed by acceleration / deceleration or diffusion of liquid while rotation is stopped.

  The connecting passage 27 is formed with a passage width of 0.5 mm to 2 mm and a depth of 50 μm to 200 μm, but there is no particular limitation as long as the inside of the connecting passage 27 can be filled with a liquid by capillary force.

  As shown in FIG. 22 (b), as shown in FIG. 22 (b), as shown in FIG. 22 (b), the mixed liquid fills the connecting passage 27 with the mixed liquid as shown in FIG. The liquid in the mixing chamber 16 can be transferred to the measurement cell 28.

  If the sample solution is blood, the dilute solution and blood cell components are mixed in the mixing chamber 16, whereby blood cells are destroyed and hemolyzed, and hemoglobin in the blood cells is mixed with the dilute solution. The diluted hemoglobin is measured for absorbance in a measurement cell, whereby the hemoglobin concentration in the blood can be calculated.

  According to the analysis device 601 and the analysis apparatus using the same according to the sixth embodiment, in the analysis device of the first embodiment, the solid component and the diluting solution communicate with the holding channel. Alternatively, the apparatus further includes a mixing chamber 16 for mixing the reagent solution, and a dilution solution chamber 22 that communicates with the mixing chamber and stores the diluted solution or the reagent solution, and is further connected to the separation chamber. The solution component holding channel 36 for holding a part of the separated solution component of the sample solution in the separation chamber, the solution component filled in the solution component holding channel, and the solution component And the reagent are mixed / reacted, and the measurement cell 37 for measuring the absorbance and turbidity of the mixed liquid is provided. Thus, according to the same process as in the first and fourth embodiments, Centrifuge A necessary amount of the subsequent solid component or high-concentration solid component solution can be transferred to the holding channel and the liquid component can be transferred to the measurement cell 37. Further, the solid component transferred to the holding channel can be diluted with the diluted solution or It can be mixed with the reagent solution and transferred to the measuring cell 28. In addition, when a part of the sample solution is transferred, it is possible to prevent the remaining solution from flowing into the holding channel after the follow-up, and the measurement accuracy of the analytical device can be improved.

  The analysis device according to the present invention and the analysis apparatus using the analysis device have an effect that a solid component after centrifugation or a high-concentration solid component solution can be transferred in a necessary amount, and one of the sample solutions. Solution component or solid component in an analytical device used to measure the components of biological fluids in an optical analyzer with the effect of preventing the remaining solution from flowing in later when the part is transferred This is useful as a method for collecting

Schematic diagram showing the configuration of the analysis device 101 according to the first embodiment of the present invention. The figure which shows the structure of the analyzer 1000 with which the device 101 for an analysis in Embodiment 1 of this invention is mounted | worn. FIG. 3 is a plan view showing a microchannel configuration of the analysis device 101 in the first embodiment. The figure for demonstrating the injection | pouring / separation process of the analysis device 101 in the said Embodiment 1. FIG. The figure for demonstrating the measurement process of the analysis device 101 in the said Embodiment 1, and a measurement cell filling process The top view which shows the microchannel structure of the device for analysis 201 in Embodiment 2 of this invention The figure for demonstrating the injection | pouring / separation process of the device 201 for an analysis in the said Embodiment 2. FIG. The figure for demonstrating the measurement process of the device 201 for an analysis in the said Embodiment 2, and a measurement cell filling process The top view which shows the microchannel structure of the device 301 for an analysis in Embodiment 3 of this invention The figure for demonstrating the injection | pouring / separation process of the analysis device 301 in the said Embodiment 3. FIG. The figure for demonstrating the measurement process of the device 301 for an analysis in the said Embodiment 3, and a measurement cell filling process The top view which shows the microchannel structure of the analysis device 401 in Embodiment 4 of this invention. The figure for demonstrating the injection | pouring / separation process of the analysis device 401 in the said Embodiment 4. FIG. The figure for demonstrating the measurement process of the device 401 for an analysis in the said Embodiment 4, and a measurement cell filling process FIG. 9 is a plan view showing a microchannel configuration of analysis device 501 according to Embodiment 5 of the present invention. The figure for demonstrating the injection | pouring / separation process of the analysis device 501 in the said Embodiment 5. FIG. The figure for demonstrating the measurement process of the device for analysis 501 in the said Embodiment 5. FIG. The figure for demonstrating the mixing / measurement cell filling process of the device for analysis 501 in the said Embodiment 5. FIG. The top view which shows the microchannel structure of the device 601 for analysis in Embodiment 6 of this invention The figure for demonstrating the injection | pouring / separation process of the analysis device 601 in the said Embodiment 6. FIG. The figure for demonstrating the measurement process of the device for analysis 601 in the said Embodiment 6. FIG. The figure for demonstrating the mixing / measurement cell filling process of the device 601 for analysis in the said Embodiment 6. FIG. The figure for demonstrating the separation and transfer method of the sample solution using the centrifugal force of a prior art example

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Substrate 2 Substrate 3 Adhesive layer 4a, 4b Microchannel 5a Reaction sample 5 Reaction solution 6 Light source 6a Transmitted light 7 Receiving part 8 Inlet 9 Liquid storage chamber 10 Separation chamber 11 Connection channel 12 Overflow channel 13 Holding channel 14 Connection passage 15 Overflow chamber 16 Mixing chamber 17 Sample overflow chamber 18 Air hole 19 Air hole 20 Air hole 21 Inlet 22 Diluent storage chamber 23 Weighing chamber 24 Dilution liquid overflow chamber 25 Capillary flow path 26 Connection path 27 Connection path 28 Measurement Cell 29 Air Hole 30 Coupling Portion 31 Solution Component 32 Solid Component 33 Air Hole 34 Connection Passage 35 Air Hole 36 Solution Component Holding Channel 37 Measurement Cell 81 Large Fluid Chamber 82 Measuring Chamber 83 Overflow Chamber 84 Receiving Chamber 85 Capillary Connection Means 86 Siphon 101, 201, 301, 401, 501, 601 Analytical device 102 Motor 103 Light source 104 traverse motor 105 a feed screw 106 servo control circuit 107 photodetector 108 adjustment circuit 109 A / D converter 110 transmitted light amount signal processing circuit 111 CPU
112 Display unit 113 Memory 1000 Analyzer

Claims (7)

In an analytical device that contains a sample solution to be analyzed and can transport the sample solution therein,
A separation chamber for separating the sample solution into a solution component and a solid component using a centrifugal force generated by rotation of the analytical device, and a part of the solid component separated in the separation chamber The overflow is connected by a connection channel for transferring the sample solution in the separation chamber, which is provided between the holding channel and the separation chamber. A solid component separated in the separation chamber after the solution component separated in the separation chamber and existing in the connection passage preferentially flows into the overflow channel. The overflow flow path is filled via the passage, and then the solid component in the separation chamber flows into the holding flow path from the connection passage via the overflow flow path. An analytical device characterized in that the part is held. The analytical device according to claim 1,
An overflow chamber for discharging the sample solution filled in the overflow channel is further provided, and the overflow chamber is connected to the overflow channel via a coupling portion, and is connected to the connection channel. The sample solution is transferred to the overflow channel by a capillary force, and an opening area of the overflow channel in the coupling portion between the overflow channel and the overflow chamber. Is larger than the opening area of the holding flow path. The analytical device according to claim 1,
Wherein the overflow chamber of the sample solution filled in the overflow channel in is discharged, further wherein the overflow chamber is connected to the overflow channel via a coupling portion, continuous Yuiryuro The sample solution is transferred from the overflow channel to the overflow channel by pressure, and the air holes provided in the overflow chamber are sucked into the overflow channel and the connection passage by the suction pump. Air is sucked, and the solution component existing in the connection passage is preferentially transferred to the coupling portion with the overflow chamber of the overflow channel by a pressure difference generated by the suction. Analytical device to be used. The analytical device according to claim 1,
A mixing chamber that communicates with the holding channel and mixes the solid component with a diluting solution or a reagent solution; a diluting solution storage chamber that communicates with the mixing chamber and contains the diluting solution or the reagent solution; A device for analysis, further comprising: The analytical device according to claim 1,
The holding channel measures the solid component according to the volume of the channel. An analysis apparatus to which the analysis device according to claim 2 is attached,
Rotating drive means for rotating the analytical device about its axis, and rotating the analytical device in a state where the sample solution is accommodated in the separation chamber, thereby converting the sample solution into a solution component and a solid component. separated, by stopping the rotation of the analysis device thereafter, the solution components which has flowed into the connecting passage from the separation chamber, the coupling portion between the overflow chamber of the overflow channel by capillary forces preferentially transferred, then the separation chamber of the solid component was transferred into the holding channel by capillary forces, is again filled the devices for analysis is rotated, the overflow channel and the connecting portion An analysis apparatus, wherein the solution component and the solid component are discharged to the overflow chamber. An analysis apparatus to which the analysis device according to claim 3 is attached,
Rotation driving means for rotating the analysis device around an axis, a pump for sucking the liquid in the analysis device, and a coupling mechanism for connecting the pump and an air hole of the analysis device the equipped, in a state where the sample solution is received in the separation chamber, by rotating the device for the analysis, the sample solution was separated into a solution component and a solid component, followed by a rotation of the analysis device After stopping, the connection mechanism is connected to the air hole of the overflow chamber, the air is sucked by the pump, and the solution component flowing into the connection passage is allowed to flow into the overflow channel in the overflow channel. The preferential transport to the coupling part with the chamber, and after the coupling part is filled, the coupling mechanism is connected to the air hole of the holding chamber coupled to the holding channel, and the pump is connected to the pump. Inhale air It is the solid component of the separation chamber is transferred to the holding channel, after the holding channel is filled with the solid component, the connecting mechanism is a leaving, by again rotating device for the analysis, the An analysis apparatus characterized in that a solution component and a solid component filled in an overflow channel and a coupling portion thereof are discharged to the overflow chamber.

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