JP4648121B2 - Automatic blood collection device, breeding system, and automated blood collection method for domestic animals - Google Patents

Description

The present invention relates to an automatic blood collection apparatus and a breeding system that can collect blood from a subject such as a mouse at predetermined intervals, and an automatic blood collection method for domestic animals .

  In a pathological test, for example, as a method for collecting blood from a mouse, which is a subject, for example, a blood sample is generally collected from the side of the eye or the tail through a person. Therefore, it is difficult to automate blood collection.

  For this reason, in a conventional automatic blood collection device, it has been proposed to always connect a blood collection tube to a mouse and collect blood every predetermined time (Patent Document 1).

Special table 2004-514495 gazette

  However, when blood is collected through human intervention, it is essential to restrain the mouse temporarily, and there is a problem that blood can only be collected in a stressed state. In addition, there is a problem that there is a pain caused by the injection needle at the time of blood collection, which is not the best blood collection method.

  In the case of Patent Document 1, blood can be collected automatically at a predetermined time, but since the mouse is not completely released, the mouse is not bred completely in a stress-free state. There is a problem that it cannot be said that the general state is reproduced.

In view of the above problems, an object of the present invention is to provide an automatic blood collection apparatus and a breeding system capable of automatically collecting blood in an environment where a subject such as a mouse can move freely, and an automatic blood collection method for domestic animals. To do.

  A first invention of the present invention for solving the above-described problem is a residence field where a subject stays, a sensor provided in the residence field for detecting a blood flow bypass portion of the subject, An automatic blood collection device comprising a blood collection jig for collecting blood.

  According to a second aspect of the present invention, in the first aspect, the staying area is a feeding / water supply area that includes a nozzle that performs feeding / water supply at a tip portion and has an entry portion into which a subject enters. In the automatic blood collection device.

  According to a third aspect of the present invention, there is provided the automatic blood collection device according to the first aspect, wherein the blood flow bypass section is provided on the back surface of the subject.

  According to a fourth aspect of the present invention, in the first aspect of the invention, the blood flow bypass unit has a marking detected by a sensor.

  According to a fifth invention, in the first invention, the blood collection jig is configured to allow a sensor for confirming a position of the blood flow bypass portion of the subject and an injection needle to be inserted into and removed from the blood flow bypass portion by an actuator. And an automatic blood collecting device.

  A sixth invention is characterized in that the automatic blood collecting device according to any one of the first to fifth inventions is provided in at least one place and has a control device for controlling blood collection of the automatic blood collecting device. In the rearing system.

The seventh invention is characterized in that a part of the blood vessel of the subject is bypassed to the body surface to form a blood flow bypass unit, and blood is collected by a blood blood collecting jig at a place where the subject stays at the time of feeding / water supply. And an automatic blood collection method for domestic animals .

  According to the present invention, blood flow can be collected by grasping a blood flow bypass portion of a subject with a sensor at a location where the subject stays. Thereby, blood can be collected without restraining the subject.

  Hereinafter, the present invention will be described in detail with reference to the drawings. Note that the present invention is not limited to the embodiments. In addition, constituent elements in the following embodiments include those that can be easily assumed by those skilled in the art or those that are substantially the same.

An automatic blood collection apparatus according to an embodiment of the present invention will be described with reference to the drawings.
FIG. 1 is a conceptual diagram illustrating an automatic blood collection device according to an embodiment. FIG. 2 is a schematic perspective view of the breeding room.
As shown in FIGS. 1 and 2, an automatic blood collection device 10 according to the present embodiment includes a nozzle 12 that feeds and feeds water 11 at a distal end portion and an entry portion 14 into which a mouse 13 that is a subject enters. For example, a color sensor 17 for confirming the position of the blood flow bypass unit 16 provided on the back side of the mouse 13 installed on the top side of the feed / water supply unit 15 and the blood feed bypass unit 15 And a blood collection device 20 that allows an injection needle 18 to be inserted and removed by an actuator 19.

A blood flow bypass unit 16 is bypassed by bypassing a part of the blood vessel of the mouse 13 as the subject to the body surface, and a blood sampler 20 is installed at a place where the mouse 13 always stays, such as a feeding / water supply unit 15. The position of the blood flow bypass unit 16 of the mouse 13 can be grasped by the color sensor 17 or the like, and blood can be collected at a desired time by the blood collector 20.
Examples of the blood collection device 20 include a capillary tube and a vacuum tube blood collection jig. By collecting blood with these blood collection means, blood collection can be automatically performed without restraining the mouse 13 and applying stress. It becomes possible.

  As a result, as compared with the case where a tube for collecting blood is connected as in the prior art, there is no stress in the behavior during breeding of the subject mouse 13, and blood collection in a normal growth state is always performed. It becomes possible.

As an example of the blood flow bypass unit 16, a blood collection jig 21 as shown in FIG.
Here, the blood collection jig 21 includes a catheter 23 that introduces blood 22 from the blood vessel of the mouse 13, a retention portion 24 that retains the blood 22 therein, and a marking 25 for the color sensor 17.
In this embodiment, a color sensor is used as the sensor. However, the present invention is not limited to this, and may be an image recognition device such as a CCD for recognizing a mouse image.

  Further, in the present invention, as shown in FIG. 2, the automatic blood collection device 10 as described above is provided at one corner of the breeding room 30, and water is supplied from the water bottle 31 to the water supply nozzle 12 through the water supply pipe 32. Thus, in such a state, when the mouse 13 enters the automatic blood collection device 10, the information of the color sensor 17 is judged by the automatic control device 33, and it is confirmed that the mouse is stopped for a predetermined time. In addition, blood is collected by the blood collecting device 20.

As a result, the automatic control device 33 can automatically perform blood sampling every predetermined time.
For example, when blood is collected about once a day, if the blood can be collected in about 2 hours, it is possible to grasp the breeding status of the subject.

  Thus, according to the present invention, it is possible to provide an automatic blood collection system that can collect blood in a state where the mouse 13 is completely released.

  For this purpose, first, in the present invention, as shown in FIG. 2, in the breeding room 30, the automatic blood collection device 10 can be installed at a place where a mouse always stays, such as a feeding / water supply station. Then, as a cylindrical feeding / water feeder 15 that feeds and drinks only in a specific posture, the mouse 13 is entered from the entry portion 14 and, for example, a color sensor is used for the bottom of the mouse 13 while feeding / watering. Judge at 17. Then, blood is collected while the mouse 13 stays in a specific posture in the feed / water feeder 15.

  In this blood collection, a part of the mouse blood flow (blood vessel) is bypassed to the body surface, the blood flow bypass unit 16 that is a blood reservoir position is grasped by the color sensor 17 or the like, and automatic blood collection is performed.

  Moreover, the pain at the time of blood collection is eliminated as a result of bypassing the blood vessel of the mouse 13 to the body surface like the blood flow bypass unit 16. As a result, it is possible to solve the problem of learning pain in the blood collection place and not being close to the blood collection place as in the prior art.

For this reason, it is desirable to use a capillary tube, a vacuum tube blood collection jig, or the like for the blood collection device 20 so that blood is automatically sucked and blood is collected just by inserting an injection needle.
It is desirable to change the needle every time. This is to prevent contamination of blood collection.

  With the above configuration, automatic blood collection from the mouse 13 becomes possible. As a result, unlike the restraint of the mouse, which is necessary for manual blood collection, blood can be collected under conditions that do not deviate from the test environment. It becomes possible to analyze.

Here, the total blood volume of the mouse is 1.4 to 3.1 ml, for example, when the weight of the adult mouse is about 18 to 40 g. Therefore, the blood collection allowable amount may be appropriately set because the blood collection allowable amount varies depending on the purpose of the test and the blood collection cycle. In addition, since a general blood collection amount is about 100 to 200 μl, blood collection is completed by a momentary blood collection operation.
In the present embodiment, the mouse is exemplified as the subject, but the subject is not limited to the mouse. Further, although one automatic blood collection device 10 is installed in the breeding room 30, the present invention is not limited to this, and it may be installed at several locations.

In addition, for example, in research on pathogens that humans may contact and infect, it is desired to collect blood in an isolated state. In such a case, the automatic blood collection device of the present invention is suitable. . Furthermore, in the case of unmanned breeding experiments in outer space, it is particularly difficult to collect blood, but by using the automatic blood collecting device of the present invention, blood can be collected at a desired interval even in unmanned experiments in outer space. Can do.
In particular, in the space environment, since it is essential to collect blood in a state free from restraint stress, the device of the present invention is particularly promising.

Next, an example of the sampling process will be described with reference to FIG.
First, the mouse 13 is sensed by the color sensor 17 (S101).
It is confirmed that it stays for a certain time (S102).
Confirmation is based on the presence or absence of changes in mouse posture.
If the posture does not change (S102, YES), the actuator is started to operate (S103).
On the other hand, when there is a change (S102, NO), the color sensor 17 senses the mouse again (S101).
Next, blood is collected with the blood collection jig 21 (S104).
After the blood collection is completed, the blood collection tube and the injection needle are replaced to prepare for the next blood collection (S105).
Wait for a certain time (S106).
By repeating this operation, blood can be collected as many times as desired.

As described above, the automatic blood collection device and the breeding system according to the present invention, and the automated blood collection method for domestic animals are an automatic blood collection device capable of automatically collecting blood in an environment where a subject such as a mouse can move freely. It is suitable for systems and automated blood collection methods for domestic animals, and is useful for tests in outer space.

It is a conceptual diagram which shows the automatic blood collection apparatus which concerns on an Example. It is a perspective schematic diagram of the breeding room concerning an example. It is a block diagram of the automatic blood collection device bypass part which concerns on an Example. It is process drawing of the automatic blood collection which concerns on an Example.

Explanation of symbols

DESCRIPTION OF SYMBOLS 10 Automatic blood collection device 11 Feeding / water supply 12 Nozzle 13 Mouse 14 Entrance part 15 Feeding / water supply device 16 Blood flow bypass part 17 Color sensor 18 Injection needle 19 Actuator 20 Blood collecting device 21 Blood collection jig 22 Blood 23 Catheter 24 Retention part 25 Marking 30 Breeding room 31 Water bottle 32 Water supply pipe 33 Automatic controller

Claims (7)

A staying place where the subject stays,
A sensor provided in the staying area for detecting a blood flow bypass portion of the subject;
An automatic blood collection apparatus comprising a blood collection jig for collecting blood of a subject. In claim 1,
An automatic blood collecting apparatus, wherein the staying place is a feeding / water supply place having a nozzle for feeding / watering at a tip part and having an entry part into which a subject enters. In claim 1,
An automatic blood collection device, wherein the blood flow bypass unit is provided on a back surface of a subject. In claim 1,
The automatic blood collection device, wherein the blood flow bypass unit has a marking detected by a sensor. In claim 1,
The blood collection jig, a sensor for confirming the position of the blood flow bypass part of the subject,
An automatic blood collection device comprising: a blood collection device that allows an injection needle to be freely inserted into and removed from the blood flow bypass portion by an actuator. The automatic blood collection device according to any one of claims 1 to 5 is provided in at least one place,
A breeding system comprising a control device for controlling blood collection of the automatic blood collection device. Bypassing the blood vessels of breeding animals in a part surface and blood flow bypass section, during the feeding-water field, automatic domestic animals, which comprises blood by the blood bled jig at the location where the subject stays Blood collection method.

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