JP4183066B2 - Magnesium, zinc, copper absorption enhancer - Google Patents

Description

[0001]
[Technical field to which the invention belongs]
The present invention relates to a magnesium, zinc or copper absorption enhancing composition.
[0002]
[Prior art]
Although dietary habits have improved, especially Japanese intake of magnesium, zinc and copper is below the nutritional requirement (Non-patent Document 1), and there is a need for improvement in that respect. However, under the Japanese dietary habits, it is difficult to sufficiently consume these inorganic components.
On the other hand, recently, an industrial production method of difructose anhydride III (hereinafter sometimes referred to as DFA III), which is an indigestible disaccharide, has been developed, and stable supply has become possible. And it was revealed that DFAIII has a bifidobacteria growth action as a physiological activity (Patent Document 1), and that DFAIII has a calcium absorption enhancing action (Patent Document 2). However, nothing is known about the absorption enhancing action of magnesium, zinc and copper.
[0003]
[Non-Patent Document 1]
Journal of Nutritional Science and Vitaminalogy 45, 519-532 [Patent Document 1]
3-5788
[Patent Document 2]
JP 11-43438
[0004]
[Problems to be solved by the invention]
In view of the present state of the art, the present invention is considerably difficult to obtain the necessary amount of magnesium, zinc and copper from a normal Japanese diet, especially infants, elderly people, patients with illness or prognosis. Again, we paid attention to the point that it is very difficult for people who cannot eat enough food. Do not increase the amount of these ingredients in the meal, but keep it as usual during the meal, except that the system enhances absorption of magnesium, zinc, and copper in the body, in other words, ingested magnesium, zinc And a new issue to develop a system that effectively uses copper in the body.
[0005]
[Means for Solving the Problems]
The present invention has been made in order to solve the above-mentioned problems. As a result of diligent research from various directions, the present inventors have an effect that DFA enhances absorption of magnesium, zinc and copper in the body. As a result, the present invention has been completed.
That is, the present invention is a magnesium, zinc or copper absorption enhancing composition characterized by containing difructose anhydride as an active ingredient. This composition can also be used as a pharmaceutical or a food or drink.
[0006]
DETAILED DESCRIPTION OF THE INVENTION
The present invention will be described in detail below.
The active ingredient of the composition according to the present invention is difructose anhydride (DFA), among which DFAIII and DFAIV are preferred. DFAIII (difructose anhydride III) is a disaccharide in which two molecules of fructose are linked by 1,2 ′ and 2,3 ′ (di-D-fructofuranose-1,2 ′: 2,3′dianhydride), Although it is highly soluble in water, sweetness is an indigestible disaccharide having about 1/2 of sucrose.
[0007]
Conventionally, DFAIII has not had an efficient industrial process. Recently, however, Inulin II (an enzyme that cleaves at the disaccharide unit from the reducing end, especially Arthrobacter sp. H65-7 (Hokkaido Univ.) And Arthrobacter globiformis C11-1 (AIST), etc.) From which the highly active enzyme can be produced industrially, and the DFAIII thus produced can be used as an active ingredient of the present invention. . Commercially available products can also be used.
[0008]
The composition which concerns on this invention contains DFA as an active ingredient, Comprising: It can utilize as a composition of a pharmaceutical or food-drinks (including animal feed). As human or veterinary medicines and foods and drinks, for example, various types of compositions such as prepared milk powder, enteral nutrients, health foods and drinks, and feed additives can be used practically. Moreover, in this composition, you may use together with the raw material which has another mineral absorption promotion effect | action, for example, maltitol, lactose, fructooligosaccharide, casein phosphopeptide (CPP), and a brewer's yeast cell wall.
The content of DFA is not particularly limited, but is usually 0.1 to 99% in the composition.
[0009]
When used as a food or drink, DFA or a processed product thereof can be used as it is, or taken together with other foods or food ingredients, and used according to conventional methods as appropriate. The processed product is a processed food containing the food, and broadly means from a clear food (in a narrow sense) to a tablet system. The composition according to the present invention may be solid (powder, granule, etc.), paste, liquid or suspension. Moreover, it can also be formulated into a health drink using various ingredients commonly used in the production of sweeteners, acidulants, vitamins and other drinks.
[0010]
When administered as a pharmaceutical composition, it can be orally administered in various forms such as tablets, capsules, granules, powders, syrups and the like. These various preparations are usually used in the pharmaceutical formulation technical field such as excipients, binders, disintegrants, lubricants, flavoring agents, solubilizers, suspension agents, coating agents, etc. It can be formulated using known adjuvants. The amount to be used varies depending on symptoms, age, body weight, administration method and dosage form, but usually 0.01 mg to 1000 mg per kg can be administered to an adult. Is similarly administered in the range of 0.5 mg to 2000 mg, preferably 1 mg to 1000 mg.
Moreover, DFA which is this active ingredient has no safety problem. As a result of a 10-day acute toxicity test using DFAIII mice, no death was observed even after oral administration of 1000 mg / kg.
[0011]
【Example】
Hereinafter, the present invention will be described with reference to examples.
Example 1
The magnesium absorption enhancing action of DFAIII by the Ussing chamber was confirmed by the following.
Sprague-Dawley male rats (average weight of about 260 g) aged 7 to 8 weeks were used for the test. Rats were purchased at 6 weeks of age (Japan SLC Co., Ltd.) and bred with commercial solid feed (CE-2, Japan Claire Co., Ltd.) until the experimental day.
On the day of the experiment, pentobarbital sodium was administered intraperitoneally to rats, and the small intestine (jejunum and ileum) and large intestine (cecum and colon) were removed under drunk conditions. After the contents of each intestinal tract were removed and deployed, the serosa and smooth muscle layers were peeled off to prepare a specimen consisting of mucosa and submucosa and mounted in a Ussing chamber (effective area 0.67 cm ² ).
On the other hand, a mucosa side buffer solution and a serosa side buffer solution were prepared. The mucous membrane side buffer is based on the following composition, and the concentration of ion is set to 0.625 mM when measuring the Mg absorption rate, and the buffer is prepared by adding DFAIII to each of 0, 1, 10, and 100 mM. I did it. The case where DFAIII was not added (0 mM) was used as a control.
[0012]
Mucosal buffer:
(30 mM HEPES buffer, pH 7.4)
NaCl 125 mM
KCl 14 mM
L-Glutamine 6 mM
MgCl · 6 ₂ O 0.625 mM
HEPES 30 mM
DFAIII 0-100 mM
[0013]
The serosa-side buffer was based on the following composition, and the ion concentration was 1 mM when measuring the Mg absorption rate.
Serosa buffer:
(30 mM HEPES buffer, pH 7.4)
NaCl 125 mM
KCl 14 mM
L-Glutamine 6 mM
MgCl · 6HH ₂ O 1 mM
HEPES 30 mM
[0014]
The entire chamber system was warmed to 37 ° C., and incubated for 30 minutes while 100% O ₂ was bubbled through the mucosa and serosa side solutions.
After completion of the incubation, the serosa-side solution was collected, and the Mg concentration in the solution was measured with a commercially available kit (Wako Pure Chemical Industries). The net mineral absorption rate per minute from the mucous membrane side to the serosa side was determined from the amount of increase in Mg concentration and expressed as a value per unit area. In addition, the test was performed in 6 series.
The obtained results are shown in FIG. As the DFAIII concentration increases, the Mg absorption is clearly increased.
[0015]
Example 2
In the mucosal buffer, ZnSO ₄ .7H ₂ O 0.0125 mM was used instead of MgCl · 6H ₂ O 1 mM, the ion concentration was 0.0125 mM in the measurement of the Zn absorption rate, and ZnSO was used as the serosa buffer. _The rate of enhanced zinc absorption was measured according to Example 1 except that ₄ · 7H ₂ O 1 mM was used.
The results obtained are represented in FIG. As the DFAIII concentration increases, the Zn absorption clearly increases.
[0016]
Example 3
In the mucosal buffer, CuCl ₂ · 2H ₂ O 0.0125 mM was used instead of MgCl · 6H ₂ O 1 mM, the ion concentration was 0.0125 mM in the measurement of the Cu absorption rate, and CuCl was used as the serosa buffer. _The rate of enhanced zinc absorption was measured according to Example 1 except that 1 mM of ₂ · 2H ₂ O was used.
The obtained results are shown in FIG. As the DFAIII concentration increases, the Cu absorption clearly increases.
[0017]
【The invention's effect】
According to the present invention, by using DFA, an excellent effect of enhancing the absorption of Mg, Zn, or Cu is achieved, and the absorption of these minerals into the body can be promoted safely and greatly.
[Brief description of the drawings]
BRIEF DESCRIPTION OF DRAWINGS FIG. 1 shows enhanced magnesium absorption of DFAIII in the jejunum, ileum, cecum and colon.
FIG. 2 shows enhanced zinc absorption of DFAIII in the jejunum, ileum, cecum and colon.
FIG. 3 is a graph showing enhanced copper absorption of DFAIII in the jejunum, ileum, cecum and colon.

Claims (3)

Magnesium, wherein the active ingredient and to Rukoto a difructose anhydride, zinc or copper absorption enhancing agent.   Magnesium, zinc or copper and difructose A magnesium, zinc or copper absorption enhancer comprising an hydride as an active ingredient. The magnesium, zinc or copper absorption enhancer according to claim 1, which is a pharmaceutical product for human or animal use.

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