JP4177424B2 - Rotation of organic material, fermentation treatment method and rot, fermentation treatment agent used therefor - Google Patents

Description

  The present invention is, for example, an organic material to be decomposed, which decomposes the organic material by subjecting the organic material to be processed, such as vegetables, fish, and meat, to rot and fermentation. It relates to a treating agent.

  Conventionally, foods such as vegetables, fish, and meat that have not been eaten or eaten at general households or restaurants are discharged as waste, and such food waste is incinerated or buried in the soil. Have been processed. In particular, in recent years, many kinds of foods are prepared using many kinds of foods, a large amount of foods that have not been eaten or eaten are discharged, and the amount of food waste tends to increase. In order to process a large amount of food waste discharged in this way, an incineration facility having a large processing capacity is required, or land for embedding and embedding work are required. For this reason, efforts are being made to reduce food waste emissions or promote recycling.

For example, a process of carbonizing used disposable chopsticks to form a carbon material having a hole, a process of enclosing microorganisms in the hole of the carbon material, a rice husk on the carbon material for food waste or livestock dung There is known a waste treatment method including a mixing step and a step of stirring a carbonaceous material and rice husk in which microorganisms are encapsulated in the food waste or livestock dung (see, for example, Patent Document 1). By this waste disposal method, it is possible to promote garbage recycling and livestock excrement recycling, reduce the amount of food waste and livestock excrement, and effectively use used disposable chopsticks.
JP 2006-136829 A (pages 2 and 3)

  In the waste treatment method described in Patent Document 1, since an aerobic ambient temperature microorganism is used as a microorganism, the atmosphere when food waste or livestock excrement, which is an organic treatment object, is decomposed is aerobic. Then, the action of aerobic cold microorganisms is promoted. However, the micropores of bamboo charcoal are low in oxygen and in an anaerobic state. Further, when food waste or animal dung is decomposed by aerobic normal temperature microorganisms in the bamboo charcoal, oxygen is consumed and the anaerobic state is promoted. For this reason, the action of the aerobic room temperature microorganisms is hindered, and the decomposition of food waste or livestock dung is delayed or stopped. In addition, only the fermentation and decomposition by aerobic room temperature microorganisms slows the rate of fermentation and decomposition, and the organic material to be processed cannot be rapidly decomposed.

  The present invention has been made by paying attention to such problems existing in the prior art, and the object of the present invention is to provide an organic workpiece that can effectively and quickly decompose the organic workpiece. It is an object to provide a method for rot and fermentation treatment, and a rot and fermentation treatment agent used therefor.

In order to achieve the above-mentioned object, the method for rot and fermentation treatment of an organic material to be processed according to claim 1 includes a microorganism that exhibits a function of fermenting and decomposing rot bacteria and rot products on bamboo charcoal. rot obtained by fermentation treatment agent performs contaminating spoilage process and fermentation process to organic treatment object, upon decomposing the organic treatment object, lactic acid as a pre-Symbol microorganism, photosynthetic bacteria, yeast, Actinomyces and A microorganism group containing a filamentous fungus is used.

The method for rot and fermentation treatment of an organic material to be processed according to claim 2 is the invention according to claim 1, wherein the rot fungus grows in the fine pores of porous bamboo charcoal. It is characterized by.

According to a third aspect of the present invention, there is provided a method for rot and fermentation treatment of an organic material according to the second aspect of the present invention. It is an opportunistic bacteria that grows inside.

The rot and fermentation treatment agent of the organic material to be processed according to the invention of claim 4 is the rot and fermentation treatment agent used in the rot and fermentation treatment method of the organic material to be processed according to claim 1, are those obtained by incorporating a microorganism that exerts function of decomposing by the putrefactive bacteria and spoilage products fermented using lactic acid bacteria, photosynthetic bacteria, yeast, and microorganisms containing actinomycetes and filamentous fungi as before Symbol microorganism It is characterized by this.

According to the present invention, the following effects can be exhibited.
In the method for rot and fermentation treatment of an organic material to be processed according to claim 1, the rot and fermentation treatment agent containing a microorganism exhibiting a function of fermenting and decomposing rot bacteria and rot products in bamboo charcoal is organically treated. It mixes with a to-be-processed object, and a decay process and a fermentation process are performed. In this case, the lactic acid bacteria as micro-organisms, photosynthetic bacteria, yeast, and microorganisms containing actinomycetes and filamentous fungi used. Therefore, the fine pores in the charcoal is a porous material, spoilage bacteria and the microorganisms are present in or under aerobic atmosphere anaerobic atmosphere. Then, the fine pores in the charcoal, the organic treatment object rot product is promoted rot by rot fungus is generated, the putrefaction products are fermented by the microorganisms, are considered to be degraded . Therefore, the organic material to be treated can be effectively and quickly decomposed by the spoilage treatment and the fermentation treatment.

In the method for rot and fermentation treatment of an organic material to be treated according to claim 2, since the rot bacteria are grown in the fine pores of porous bamboo charcoal, the effect of the invention according to claim 1 In addition, the effect of spoilage bacteria can be remarkably improved in the fine pores of bamboo charcoal having a large surface area.

In the method for rot and fermentation of an organic material to be treated according to claim 3, the rot is an opportunistic bacterium that grows in the fine pores of porous bamboo charcoal by leaving bamboo charcoal in a fine powder. In addition to the effect of the invention according to claim 2, the rot bacteria can be held in the bamboo charcoal by a simple operation.

The rot and fermentation treatment agent of the organic material to be processed according to claim 4 is used in the method for rot and fermentation treatment of an organic material to be processed according to claim 1, wherein Contains organisms. In this case, the lactic acid bacteria as micro-organisms, photosynthetic bacteria, yeast, and microorganisms containing actinomycetes and filamentous fungi used. For this reason, the spoilage and fermentation treatment agent is suitably used in the spoilage and fermentation treatment method of the organic object to be treated according to claim 1, and can exhibit the effect based on claim 1.

In the following, embodiments that are considered to be the best of the present invention will be described in detail.
In the present embodiment, the organic matter to be rotted and fermented by the fermenting method include rot that contains a microorganism (hereinafter referred to as a fermenting microorganism) that exhibits the function of fermenting and decomposing rot bacteria and rot products on bamboo charcoal. A fermenting agent is mixed into an organic material to be processed to perform rot processing and fermentation processing. Organic processed materials include vegetables such as spinach, cabbage and lettuce, fish such as mackerel (sardine) and sanma (autumn fish), meat such as pork, beef and chicken, and seasonings such as miso and soy sauce. Examples include cooking waste and leftover food waste. Such an organic to-be-processed object is the food waste produced | generated in large quantities in the general household or the restaurant, and discharged | emitted without eating and drinking. Furthermore, examples of organic materials include biological manure, sludge from rivers and seas, and miscellaneous wastewater from households.

Such an organic material to be treated contains organic substances such as carbohydrates, proteins, and lipids (oils and fats). Carbohydrate is an organic compound having a similar structure including saccharides. Basically, it has a general formula represented by C _n (H ₂ O) _m . A protein has a structure in which a plurality of amino acids form a peptide bond between a carboxyl group and an amino group and are linked in a straight chain. Lipids are composed of fatty acid and glycerol esters.

  Bamboo charcoal is a porous material that has a function of mainly supporting spoilage bacteria and fermentation microorganisms, and also has a property of adsorbing organic materials, ammonia, amines, and the like. This bamboo charcoal is obtained by heating bamboo such as broken bamboo, true bamboo, and moso bamboo to, for example, 600 to 800 ° C. to complete combustion and carbonization. Similarly, charcoal as a porous material can be mixed with bamboo charcoal as a porous material. Such charcoal can be obtained by carbonizing wood such as beech, oak and kunugi, and thinned wood such as cedar and cypress under the same conditions as bamboo charcoal. These bamboo charcoal or charcoal is usually used as a powder or granular material having an average particle diameter of about 100 μm to 10 mm. When the average particle diameter of bamboo charcoal or charcoal is less than 100 μm, it is difficult to produce bamboo charcoal or charcoal, and when it exceeds 10 mm, the dispersibility of bamboo charcoal or charcoal with respect to the organic object to be treated is not preferable.

  Bamboo charcoal tends to be more porous than charcoal and the like, but the diameter of these micropores is about 0.1 μm to 100 μm. Moreover, the micropores having different sizes are distributed, and the functions of the spoilage bacteria and the fermentation microorganism are more effectively exhibited. If the diameter of the micropores is less than 0.1 μm, it becomes difficult for rot bacteria, microorganisms, and organic treatments to penetrate into the micropores. On the other hand, if the diameter exceeds 100 μm, the micropores become large and decay in an anaerobic atmosphere. In addition, the fermentation treatment ability is lowered, and the number of micropores is reduced, so that the efficiency of the decay and fermentation treatment is lowered.

Subsequently, the spoilage bacteria rot the organic material to be treated, and exhibit a function to rot the organic material to be treated mainly under the anaerobic atmosphere of the fine pores of bamboo charcoal. The spoilage bacteria are preferably parasitic in the fine pores of the porous bamboo charcoal, and the function of the spoilage bacteria can be improved in the fine pores of the bamboo charcoal having a large surface area. Such spoilage bacteria can be propagated in the fine pores of porous bamboo charcoal by leaving bamboo charcoal in a fine powder and left in the atmosphere, and the spoilage bacteria that proliferate are usually opportunistic bacteria (opportunistic bacteria). In addition, spoilage bacteria can also be culture | cultivated on predetermined conditions and can infest in the micropore of bamboo charcoal.

  The spoilage bacteria are so-called bad bacteria, and mainly decompose proteins to produce spoilage products such as amine compounds, ammonia, indole, and phenol. This spoiled product becomes a causative substance of malodor (septic odor). Examples of spoilage bacteria include E. coli, staphylococci, and Clostridium perfringens in addition to opportunistic bacteria.

  Next, the fermentation microorganism exhibits a function of further fermenting and decomposing the organic product or the spoilage product produced by the spoilage fungus. Fermentation-type microorganisms contain about 80 types of effective microorganism groups belonging to Group 10, and are obtained by complex culture of them (useful microorganism group). These fermentation microorganisms are classified into photosynthetic microorganisms, lactic acid bacteria, nitrogen-fixing bacteria (Azotobacter), mycorrhizal fungi, radiofungi, and yeasts. Of these, photosynthetic microorganisms exist both anaerobically and aerobically, and lactic acid bacteria function anaerobically. Other nitrogen-fixing bacteria (Azotobacter), mycorrhizal fungi, radiofungi and yeast are all aerobic. Lactic acid bacteria include bifidobacteria and the like. As a group of useful microorganisms (EM fungus), those containing lactic acid bacteria, photosynthetic bacteria, yeasts, radioactive bacteria and filamentous fungi are preferable because they can exert a synergistic effect by these microorganisms.

  As the fermentation microorganism, either an anaerobic fermentation microorganism or an aerobic fermentation microorganism may be used. Since the inside of bamboo charcoal can be either anaerobic or aerobic depending on the conditions such as the atmosphere in which they are placed, both anaerobic and aerobic fermentation microorganisms can exert their functions. it can. Since there is a tendency to become an anaerobic atmosphere in the fine pores of bamboo charcoal, it is preferable that the fermentation microorganism is an anaerobic fermentation microorganism to promote the fermentation of the organic material. Representative examples of the photosynthetic microorganism include photosynthetic bacteria and cyanobacteria. By the action of this photosynthetic microorganism, organic substances are decomposed in an anaerobic atmosphere, and generated methane and the like are further decomposed. Moreover, lactic acid bacteria decompose | disassemble an organic to-be-processed object in an anaerobic atmosphere, produce | generate a lactic acid related substance, and also decompose | disassemble the lactic acid related substance. Fermentation and decomposition of an organic material to be processed by a fermentation microorganism are directly based on the functions of enzymes such as carbohydrate-degrading enzymes, proteolytic enzymes, and lipolytic enzymes secreted by the fermentation microorganisms.

The spoilage and fermentation treatment agent can be obtained by mixing the bamboo charcoal, spoilage bacteria and fermentation microorganisms. As mentioned above, the spoilage bacteria can be easily grown on bamboo charcoal. Can be obtained. The mixing ratio of the bamboo charcoal rot fungus had grown and fermentation system microorganism 1 by mass ratio: 1 to 1: about 2 are preferred. When this mass ratio is less than 1: 1, the function of the fermentation microorganism is insufficient as compared with the function of the rot bacterium, and the rot product produced by the rot bacterium tends to be not sufficiently decomposed. On the other hand, when the mass ratio exceeds 1: 2, the ratio of spoilage bacteria decreases as compared with fermentation microorganisms, and the function of the spoilage bacteria is insufficient, and the decomposition rate of the organic treatment object tends to be slow.

  In the case of subjecting organic matter to rot and fermentation, the above-mentioned rot and fermentation treatment agent containing bamboo rot and fermentation microorganisms in bamboo charcoal is mixed into the organic matter to be treated. It is preferable that the compounding quantity of the rot and fermentation treatment agent with respect to a processed material is 5 mass% or more, and can fully exhibit the effect of rot and a fermentation treatment agent at 10-20 mass%. When this compounding quantity is less than 5 mass%, the function of a rot and a fermentation processing agent cannot fully be exhibited, but a rot process or a fermentation process becomes inadequate, and is unpreferable. In the case where a rot and fermentation treatment agent is mixed into an organic material to be treated, it is desirable to stir to increase the efficiency of the rot treatment and the fermentation treatment. In that case, it is more desirable to perform stirring intermittently. Furthermore, when performing a decay process and a fermentation process, it can also heat and can accelerate | stimulate reaction.

  Judgment regarding the completion of decomposition after performing such organic matter rot and fermentation treatment is judged by the disappearance of carbon dioxide, ammonia gas, methane gas, etc. and the smell disappears and the liquid becomes transparent. The Furthermore, the total amount of organic carbon and the total amount of nitrogen can be measured by a carbon amount measuring device and a nitrogen amount measuring device, and the degree of decomposition of the organic workpiece can be quantitatively determined.

Now, the operation of the organic processing object rot and fermentation treatment method according to the present embodiment will be described. The rot and fermentation treatment agents are, for example, opportunistic bacteria grown by leaving bamboo charcoal in the atmosphere, and anaerobic fermentation microorganisms. It is obtained by mixing almost the same amount of useful microorganisms as. The obtained rot and fermentation treatment agent is mixed as, for example, a food waste as an organic material to be treated, and the rot treatment is performed by stirring. At this time, the micropores in the porous bamboo charcoal have a large surface area and are in an anaerobic atmosphere, and there are spoilage bacteria and fermentation microorganisms that effectively express the function in the anaerobic atmosphere. For this reason, the organic material to be processed is spoiled by spoilage bacteria to produce a spoiled product, ammonia gas, methane gas, and the like. Subsequently, the spoilage product, ammonia gas, methane gas, etc. are further fermented by anaerobic fermentation microorganisms, and the fermentation proceeds rapidly and decomposes into carbon dioxide (carbon dioxide), water, nitrogen gas, etc. It is presumed that this will be completed.

The effects exhibited by the above embodiment will be described collectively below.
In the method for rot and fermentation treatment of an organic object to be processed according to the embodiment, a rot and fermentation treatment agent containing bamboo rot and fermentation microorganisms in bamboo charcoal is mixed with the organic object to be subjected to rot treatment. For this reason, in the fine pores of bamboo charcoal, the organic material is spoiled by spoilage bacteria in an anaerobic or aerobic atmosphere to produce a spoiled product, which is fermented by a fermentation microorganism. Is decomposed. Therefore, the organic workpiece can be decomposed (mineralized) effectively and quickly by the synergistic action of the rot treatment and the fermentation treatment.

  Therefore, it is possible to effectively decompose the organic processing object in any atmosphere such as anaerobic atmosphere such as soil, sludge, water, etc., or an aerobic atmosphere such as air or near the water surface. It can be applied in a wide range and is very useful.

-Since the spoilage bacteria are growing in the fine pores of the porous bamboo charcoal, the effect of the spoilage bacteria can be remarkably improved in the fine pores of the bamboo charcoal having a large surface area.
-Since the spoilage bacteria is an opportunistic bacteria that is allowed to stand in the form of fine powder of bamboo charcoal and grows in the fine pores of the bamboo charcoal, the spoilage bacteria can be retained in the bamboo charcoal by a simple operation.

-Since the said fermentation type | system | group microorganisms are anaerobic fermentation type | system | group microorganisms, a fermentation type | system | group microorganisms can promote fermentation of an organic to-be-processed object in the micropore which is an anaerobic atmosphere of porous bamboo charcoal. By Anaerobic fermentation system microorganism according is microbial group (EM bacteria) fermentation of organic treatment object under anaerobic atmosphere, decompose can be further promoted. When the microorganism group (EM fungus) contains lactic acid bacteria, photosynthetic bacteria, yeasts, radioactive bacteria and filamentous fungi, a synergistic effect by a plurality of microorganisms can be exhibited.

  The rotting and fermentation treatment agent is used in the rotting and fermenting processing method of the organic material to be processed, and the bamboo charcoal contains rotting bacteria and fermentation microorganisms. It is suitably used in the method and can exert its effect.

Hereinafter, the embodiment will be described more specifically with reference to examples and comparative examples.
(Examples 1 to 3 and Comparative Examples 1 and 2, rot of milk, fermentation treatment)
As bamboo charcoal, 100 μm to 10 mm pulverized charcoal obtained by heating and combusting miscellaneous bamboo such as broken bamboo and true bamboo to 600 to 800 ° C. and carbonizing them was used. This bamboo charcoal was left in the atmosphere for one day, and opportunistic bacteria as spoilage bacteria were grown in the fine pores of the porous bamboo charcoal. On the other hand, the following useful microorganism groups (EM bacteria, Effective Microorganisms) were prepared as anaerobic fermentation microorganisms. Then, 70 ml (about 70 g) of a fermentation microorganism was mixed with 50 g of the bamboo charcoal to prepare a rot and fermentation treatment agent.

Useful microorganism group Microorganism type Main species Lactobacillus plantarum (ATCC8014)
Lactobacillus casei (ATCC7469)
Streptococcus Lactis (IFO12007)
2. Photosynthetic bacteria Rhotdopseudomonas plaustris (ATTC17001)
Rhodobacter sphaeroides (ATTC17023)
3. Yeast Saccharomyces cerevisiae (IFO0203)
Candida utilis (IFO0619)
4). Radiophile Steptomyces albus (ATCC3004)
Steptomyces griseus (IFO3358)
5. Aspergillus oryzae (IFO5770)
Mucor hiemalis (IFO8567)
6). Other useful bacteria that exist in nature and participate in the mixed culture process
Therefore, it is a useful microorganism that can survive at pH 3.5 or lower.

  Next, when 10% by mass of rotting and fermenting agent is added to milk as an organic material (Example 1), when rotting and 50% by mass of fermenting agent are added to milk (Example 2), and milk In the case where 100% by mass of the rotting and fermentation treatment agent was added (Example 3), each sample was put in a test tube, sealed and left standing. On the other hand, in the case of only milk (Comparative Example 1) and the case of adding only fermentation microorganisms to milk (Comparative Example 2), each sample is put in a test tube, left to be sealed and left after a predetermined period. Shake to mix. And the state in each test tube was observed visually. The results are shown in Table 1.

その結果、腐敗、発酵処理剤の添加量が最も多かった実施例３では、１０日後に炭酸ガス、アンモニア等のガスが発生しなくなると共に、黒い分解生成物が沈殿すると同時に水が透明化した。従って、この時点で牛乳が発酵されて分解されたものと判断した。また、腐敗、発酵処理剤の添加量が次に多かった実施例２では、１１日後にガスの発生が止まると共に、黒い分解生成物が沈殿し、水も透明化した。従って、この時点で牛乳が発酵されて分解されたものと判断した。さらに、腐敗、発酵処理剤の添加量が最も少なかった実施例１では、１２日後にガスが発生しなくなると共に、分解生成物が沈殿し、水が透明化した。従って、この時点で牛乳が発酵されて分解されたものと判断した。よって、腐敗、発酵処理剤の添加量は、牛乳の１０質量％程度でも腐敗が十分に進行することが明らかになった。このように、有機被処理物である牛乳を速やかに、しかも効果的に腐敗、発酵処理することができた。

As a result, in Example 3 where the amount of spoilage and fermentation treatment agent was the largest, gas such as carbon dioxide and ammonia was not generated after 10 days, and a black decomposition product was precipitated and water became transparent at the same time. Therefore, it was judged that milk was fermented and decomposed at this point. Further, in Example 2 where the addition amount of the rotting and fermentation treatment agent was the next largest, the generation of gas stopped after 11 days, a black decomposition product precipitated, and water became transparent. Therefore, it was judged that milk was fermented and decomposed at this point. Further, in Example 1 in which the addition amount of the spoilage and fermentation treatment agent was the smallest, gas was not generated after 12 days, the decomposition product was precipitated, and water became transparent. Therefore, it was judged that milk was fermented and decomposed at this point. Therefore, it became clear that even if the addition amount of rot and a fermentation processing agent is about 10 mass% of milk, rot will fully advance. Thus, it was possible to quickly and effectively rot and ferment milk, which is an organic material.

On the other hand, Comparative Example 1 with only milk showed only a tendency of separation of solids and moisture by 12 days, and almost no gas generation or color change was observed. In Comparative Example 2 in which only fermented microorganisms were added to milk, the stopper did not fly to the extent that a weak odor was generated by the decomposition of milk until 12 days later.
(Examples 4 and 5 and Comparative Examples 3 and 4, rot of miso, fermentation treatment)
200 ml of water was added to 100 g of miso, stirred and mixed with a mixer to prepare a sample, and 11 ml each was put into four test tubes. Then, 5 ml of the rotting and fermenting agent used in Examples 1 to 3 was added to one test tube and mixed (Example 4). In another test tube, 6.5 ml of the rotting and fermenting agent used in Examples 1 to 3 was added and mixed (Example 5). On the other hand, the thing which can put nothing in another test tube was prepared (Comparative Example 3). Further, another test tube in which only 5 ml of fermentation microorganisms were added and mixed was prepared (Comparative Example 4).

  Each prepared test tube was sealed and allowed to stand, and after a predetermined period, the test tube was shaken and mixed. And the state in each test tube was observed visually. The results are shown in Table 2.

表２に示したように、実施例４及び５では、２日後にガスが多量に発生して栓が飛んだ。さらに、実施例５では１１日後にガスの発生が止まり、栓が飛ばなくなると共に、臭気も感じなくなった。液も透明になった。また、実施例４では１３日後にガスの発生が止まり、栓が飛ばなくなると共に、臭気の発生も収まった。液も透明になった。一方、有機被処理物としての味噌のみの比較例３及び味噌に発酵系微生物のみを加えた比較例４では、若干のガスの発生や水分の分離が見られたが、大きな変化は認められなかった。
（実施例６、７及び比較例５、６、魚の腐敗、発酵処理）
魚（鯖）１００ｇに水２００ｍｌを加え、ミキサーで撹拌、混合してサンプルを調製し、４本の試験管にそれぞれ１１ｍｌずつ入れた。そして、１本の試験管に前記実施例１〜３で用いた腐敗、発酵処理剤を５ｍｌ入れて混合した（実施例６）。別の試験管に前記実施例１〜３で用いた腐敗、発酵処理剤を６．５ｍｌ入れて混合した（実施例７）。一方、さらに別の試験管に何も入れないものを用意した（比較例５）。さらにまた別の試験管に発酵系微生物のみを５ｍｌ入れて混合したものを用意した（比較例６）。

As shown in Table 2, in Examples 4 and 5, a large amount of gas was generated after 2 days and the plugs flew off. Furthermore, in Example 5, the generation of gas stopped after 11 days, the stoppers did not fly, and no odor was felt. The liquid also became transparent. Further, in Example 4, the generation of gas stopped after 13 days, the stopper stopped flying, and the generation of odor was reduced. The liquid also became transparent. On the other hand, in Comparative Example 3 with only miso as the organic material to be treated and Comparative Example 4 in which only the fermentation microorganisms were added to miso, slight gas generation and water separation were observed, but no significant change was observed. It was.
(Examples 6 and 7 and Comparative Examples 5 and 6, fish spoilage, fermentation treatment)
Samples were prepared by adding 200 ml of water to 100 g of fish (carp), stirring and mixing with a mixer, and putting 11 ml each in four test tubes. Then, 5 ml of the rotting and fermenting agent used in Examples 1 to 3 was added to one test tube and mixed (Example 6). In another test tube, 6.5 ml of the rotting and fermenting agent used in Examples 1 to 3 was added and mixed (Example 7). On the other hand, the thing which can put nothing in another test tube was prepared (Comparative Example 5). Further, another test tube in which only 5 ml of the fermentation microorganism was added and mixed was prepared (Comparative Example 6).

  Each test tube was sealed and allowed to stand, and after a predetermined period, the test tube was shaken and mixed. And the state in each test tube was observed visually. The results are shown in Table 3.

表３に示したように、実施例６及び７では、６日後にガスが多量に発生して栓が飛んだ。さらに、実施例７では１１日後にガスの発生が止まり、栓が飛ばなくなると共に、臭気も感じなくなった。液も透明になった。また、実施例６では１３日後にガスの発生が止まり、栓が飛ばなくなると共に、臭気の発生も収まった。液も透明になった。一方、魚そのままの比較例５及び魚に発酵系微生物のみを加えた比較例６では、若干のガスの発生や水分の分離が見られたが、大きな変化は認められなかった。
（実施例８、９及び比較例７、８、ほうれん草の腐敗、発酵処理）
ほうれん草１００ｇに水２００ｍｌを加え、ミキサーで撹拌、混合してサンプルを調製し、４本の試験管にそれぞれ１１ｍｌずつ入れた。そして、１本の試験管に前記実施例１〜３で用いた腐敗、発酵処理剤を５ｍｌ入れて混合した（実施例８）。別の試験管に前記実施例１〜３で用いた腐敗、発酵処理剤を６．５ｍｌ入れて混合した（実施例９）。一方、さらに別の試験管に何も入れないものを用意した（比較例７）。さらにまた別の試験管に発酵系微生物のみを５ｍｌ入れて混合したものを用意した（比較例８）。

As shown in Table 3, in Examples 6 and 7, a large amount of gas was generated after 6 days and the plugs flew off. Furthermore, in Example 7, the generation of gas stopped after 11 days, the stoppers did not fly, and no odor was felt. The liquid also became transparent. Further, in Example 6, the generation of gas stopped after 13 days, the stopper stopped flying, and the generation of odors also subsided. The liquid also became transparent. On the other hand, in Comparative Example 5 with the fish as it was and Comparative Example 6 in which only the fermentation microorganisms were added to the fish, slight gas generation and moisture separation were observed, but no significant change was observed.
(Examples 8 and 9 and Comparative Examples 7 and 8, spinach rot, fermentation treatment)
200 ml of water was added to 100 g of spinach, stirred and mixed with a mixer to prepare a sample, and 11 ml each was put into four test tubes. Then, 5 ml of the rot and fermentation treatment agent used in Examples 1 to 3 were put into one test tube and mixed (Example 8). In another test tube, 6.5 ml of the rotting and fermenting agent used in Examples 1 to 3 was added and mixed (Example 9). On the other hand, the thing which can put nothing in another test tube was prepared (Comparative Example 7). Furthermore, what mixed only 5 ml of fermentation microorganisms in another test tube was prepared (Comparative Example 8).

  Each prepared test tube was sealed and allowed to stand, and after a predetermined period, the test tube was shaken and mixed. And the state in each test tube was observed visually. The results are shown in Table 4.

表４に示したように、実施例８及び９では、６日後にガスが多量に発生して栓が飛んだ。さらに、実施例９では１１日後にガスの発生が止まり、栓が飛ばなくなると共に、臭気も感じなくなった。液も透明になった。また、実施例８では１３日後にガスの発生が止まり、栓が飛ばなくなると共に、臭気の発生も収まった。液も透明になった。一方、ほうれん草そのままの比較例７及びほうれん草に発酵系微生物のみを加えた比較例８では、若干のガスの発生や水分の分離が見られたが、それ以上の変化は認められなかった。
（実施例１０、１１及び比較例９、１０、肉の腐敗、発酵処理）
肉（豚肉）１００ｇに水２００ｍｌを加え、ミキサーで撹拌、混合してサンプルを調製し、４本の試験管にそれぞれ１１ｍｌずつ入れた。そして、１本の試験管に前記実施例１〜３で用いた腐敗、発酵処理剤を５ｍｌ入れて混合した（実施例１０）。別の試験管に前記実施例１〜３で用いた腐敗、発酵処理剤を６．５ｍｌ入れて混合した（実施例１１）。一方、さらに別の試験管に何も入れないものを用意した（比較例９）。さらにまた別の試験管に発酵系微生物のみを５ｍｌ入れて混合したものを用意した（比較例１０）。

As shown in Table 4, in Examples 8 and 9, a large amount of gas was generated after 6 days and the plugs flew off. Furthermore, in Example 9, the gas generation stopped after 11 days, the stopper stopped flying, and no odor was felt. The liquid also became transparent. Further, in Example 8, the generation of gas stopped after 13 days, the stopper stopped flying, and the generation of odors also subsided. The liquid also became transparent. On the other hand, in Comparative Example 7 in which spinach was used as it was and Comparative Example 8 in which only fermentation microorganisms were added to spinach, slight gas generation and water separation were observed, but no further changes were observed.
(Examples 10 and 11 and Comparative Examples 9 and 10, meat rot, fermentation treatment)
200 ml of water was added to 100 g of meat (pork), stirred and mixed with a mixer to prepare a sample, and 11 ml each was put into four test tubes. Then, 5 ml of the rotting and fermenting agent used in Examples 1 to 3 was added to one test tube and mixed (Example 10). In another test tube, 6.5 ml of the rotting and fermenting agent used in Examples 1 to 3 was added and mixed (Example 11). On the other hand, the thing which can put nothing in another test tube was prepared (Comparative Example 9). Furthermore, what mixed only 5 ml of fermentation type microorganisms in another test tube was prepared (Comparative Example 10).

  Each test tube was sealed and allowed to stand, and after a predetermined period, the test tube was shaken and mixed. And the state in each test tube was observed visually. The results are shown in Table 5.

表５に示したように、実施例１０及び１１では、２日後にガスが発生して栓が飛んだ。さらに、実施例１１では１１日後にガスの発生が止まり、栓が飛ばなくなると共に、臭気も感じなくなった。液も透明になった。また、実施例１０では１３日後にガスの発生が止まり、栓が飛ばなくなると共に、臭気の発生も収まった。液も透明になった。一方、肉そのままの比較例９及び肉に発酵系微生物のみを加えた比較例１０では、若干のガスの発生や水分の分離が見られたが、それ以上の変化は認められなかった。
（実施例１２、１３及び比較例１１、１２、じゃがいもの腐敗、発酵処理）
じゃがいも１００ｇに水２００ｍｌを加え、ミキサーで撹拌、混合してサンプルを調製し、４本の試験管にそれぞれ１１ｍｌずつ入れた。そして、１本の試験管に前記実施例１〜３で用いた腐敗、発酵処理剤を５ｍｌ入れて混合した（実施例１２）。別の試験管に前記実施例１〜３で用いた腐敗、発酵処理剤を６．５ｍｌ入れて混合した（実施例１３）。一方、さらに別の試験管に何も入れないものを用意した（比較例１１）。さらにまた別の試験管に発酵系微生物のみを５ｍｌ入れて混合したものを用意した（比較例１２）。

As shown in Table 5, in Examples 10 and 11, gas was generated after 2 days and the plugs flew off. Furthermore, in Example 11, the generation of gas stopped after 11 days, the stoppers did not fly, and no odor was felt. The liquid also became transparent. Further, in Example 10, the generation of gas stopped after 13 days, the stopper stopped flying, and the generation of odor was reduced. The liquid also became transparent. On the other hand, in Comparative Example 9 with the meat intact and Comparative Example 10 in which only the fermentation microorganisms were added to the meat, slight gas generation and moisture separation were observed, but no further changes were observed.
(Examples 12 and 13 and Comparative Examples 11 and 12, potato rot, fermentation treatment)
Samples were prepared by adding 200 ml of water to 100 g of potato, stirring and mixing with a mixer, and putting 11 ml each in four test tubes. Then, 5 ml of the rot and fermentation treatment agent used in Examples 1 to 3 were put into one test tube and mixed (Example 12). In another test tube, 6.5 ml of the rotting and fermenting agent used in Examples 1 to 3 was added and mixed (Example 13). On the other hand, the thing which can put nothing in another test tube was prepared (comparative example 11). Furthermore, what mixed only 5 ml of fermentation type microorganisms in another test tube was prepared (Comparative Example 12).

  Each test tube was sealed and allowed to stand, and after a predetermined period, the test tube was shaken and mixed. And the state in each test tube was observed visually. The results are shown in Table 6.

表６に示したように、実施例１２及び１３では、６日後にガスが多量に発生して栓が飛んだ。さらに、実施例１３では１１日後にガスの発生が止まり、栓が飛ばなくなると共に、臭気も感じなくなった。液も透明になった。また、実施例１２では１３日後にガスの発生が止まり、栓が飛ばなくなると共に、臭気の発生も収まった。液も透明になった。一方、じゃがいもそのままの比較例１１及びじゃがいもに発酵系微生物のみを加えた比較例１２では、若干のガスの発生や水分の分離が見られたが、その他の変化は認められなかった。
（実施例１４、牛乳の腐敗、発酵処理）
前記実施例１で日和見菌を微細孔内に寄生させた竹炭３００ｇに、実施例１で用いた有用微生物群（ＥＭ菌）６００ｍｌ（約６００ｇ）を混合して腐敗、発酵処理剤を調製した。そして、樹脂容器（ペットボトル）内に牛乳１５０ｍｌ、前記腐敗、発酵処理剤及び水６００ｍｌを入れ、常温、嫌気性雰囲気下に８３日間おいて腐敗、発酵処理を行った。その結果、ガスの発生が収まり、臭気もなくなり、黒い分解生成物が沈殿すると同時に水が透明化した。その後、樹脂容器をテドラパックに入れて減圧にし、樹脂容器を開封してガスをテドラパック内に放出した。テドラパック内のガスについて、検知管を用い二酸化炭素（ＣＯ_２）、アンモニア（ＮＨ_３）、硫化水素（Ｈ_２Ｓ）、メルカプタン類及びアミン類の濃度を分析した。ここで、メルカプタン類は、エチルメルカプタン、ブチルメルカプタン等である。アミン類は、エチルアミン、イソプロピルアミン等である。これら成分の濃度分析を２回行い、その平均ガス濃度を算出した。それらの分析結果を表７に示した。

As shown in Table 6, in Examples 12 and 13, a large amount of gas was generated after 6 days and the plugs flew off. Furthermore, in Example 13, the generation of gas stopped after 11 days, the stoppers did not fly, and no odor was felt. The liquid also became transparent. Further, in Example 12, the generation of gas stopped after 13 days, the stopper stopped flying, and the generation of odor was reduced. The liquid also became transparent. On the other hand, in Comparative Example 11 in which potatoes were used as they were and in Comparative Example 12 in which only fermentation microorganisms were added to potatoes, some gas generation and moisture separation were observed, but no other changes were observed.
(Example 14, rot of milk, fermentation treatment)
In 300 g of bamboo charcoal in which the opportunistic bacteria were infested in Example 1 above, 600 ml (about 600 g) of the useful microorganism group (EM fungus) used in Example 1 was mixed to prepare a rot and fermentation treatment agent. And 150 ml of milk, the said rot, fermentation processing agent, and 600 ml of water were put into the resin container (pet bottle), and rot and fermentation processing were performed in normal temperature and anaerobic atmosphere for 83 days. As a result, the generation of gas was stopped, the odor disappeared, the black decomposition product was precipitated, and at the same time, the water became transparent. Thereafter, the resin container was put into a tedla pack to reduce the pressure, the resin container was opened, and the gas was released into the tedra pack. The gas in the Tedorapakku, carbon dioxide _(CO 2) using a detector tube, ammonia _(NH 3), hydrogen sulfide _(H 2 S), and analyzing the concentration of mercaptans and amines. Here, the mercaptans are ethyl mercaptan, butyl mercaptan, and the like. Examples of amines include ethylamine and isopropylamine. The concentration analysis of these components was performed twice, and the average gas concentration was calculated. The analysis results are shown in Table 7.

表７の結果より、牛乳中の油脂、タンパク質、糖分中に含まれる炭素は、二酸化炭素として放出されることがわかった。また、牛乳中のタンパク質に含まれる窒素は、アンモニアとしてではなく、窒素ガスとして放出されているものと考えられる。さらに、牛乳中のタンパク質に含まれる硫黄は、硫化水素やメルカプタン類として放出されることがわかった。

From the results in Table 7, it was found that carbon contained in fats and oils, protein, and sugar in milk was released as carbon dioxide. In addition, it is considered that nitrogen contained in the protein in milk is released not as ammonia but as nitrogen gas. Furthermore, it was found that sulfur contained in protein in milk is released as hydrogen sulfide and mercaptans.

  Next, the processing liquid (septic and fermented milk) after rot and fermentation treatment is collected with a whole pipette, and the total carbon amount (mg / l), inorganic carbon amount using a carbon meter [Shimadzu Corporation, etc.] (Mg / l) and the total organic carbon amount (mg / l) were measured, and the total nitrogen amount (mg / l) was measured using a nitrogen meter [Shimadzu Corporation etc.]. For comparison, measurements were also made on untreated milk that had not been spoiled or fermented. The results are shown in Table 8.

表８に示した結果より、牛乳中の有機炭素の８７．６％が分解されて除去されると共に、牛乳中の窒素の８３．９％が分解されて除去された。
（実施例１５、河川の水の腐敗、発酵処理）
前記実施例１で日和見菌を微細孔内に寄生させた竹炭１０ｋｇに、実施例１で用いた有用微生物群（ＥＭ菌）１０リットル（約１０ｋｇ）を混合して腐敗、発酵処理剤を調製した。そして、河川（静岡県浜松市の旧二俣川）の水１トンに前記腐敗、発酵処理剤及び糖蜜１０リットルを加え、常温で１週間放置し、腐敗、発酵処理を行った。その結果、ガスの発生が収まり、臭気もなくなると共に、黒い分解生成物が沈殿し、水が透明化した。さらに、腐敗、発酵処理後の水について、亜硝酸、硝酸及びリン酸の濃度変化を各分析装置によって分析した。その結果、亜硝酸に関しては、原水が０．５（ｍｇ／ｌ）であるのに対して腐敗、発酵処理後の水は０．１（ｍｇ／ｌ）であった。硝酸に関しては、原水が２０（ｍｇ／ｌ）であるのに対して腐敗、発酵処理後の水は２（ｍｇ／ｌ）であった。リン酸に関しては、原水が０．６（ｍｇ／ｌ）であるのに対して腐敗、発酵処理後の水は０．３５（ｍｇ／ｌ）であった。このように、腐敗、発酵処理により、原水中の亜硝酸、硝酸及びリン酸の濃度を大幅に低下させることができた。

From the results shown in Table 8, 87.6% of organic carbon in milk was decomposed and removed, and 83.9% of nitrogen in milk was decomposed and removed.
(Example 15, rot of river water, fermentation treatment)
10 kg of bamboo charcoal in which the opportunistic bacteria were infested in the micropores in Example 1 above was mixed with 10 liters (about 10 kg) of the useful microorganism group (EM bacteria) used in Example 1 to prepare a rot and fermentation treatment agent. . Then, the rot, fermentation treatment agent and 10 liters of molasses were added to 1 ton of water in the river (former Futaba River in Hamamatsu City, Shizuoka Prefecture), and the mixture was allowed to stand at room temperature for 1 week for rot and fermentation treatment. As a result, generation of gas was stopped, odor was eliminated, black decomposition products were precipitated, and water became transparent. Furthermore, about the water after rot and fermentation processing, the concentration change of nitrous acid, nitric acid, and phosphoric acid was analyzed with each analyzer. As a result, regarding nitrous acid, raw water was 0.5 (mg / l), whereas water after rot and fermentation treatment was 0.1 (mg / l). Regarding nitric acid, the raw water was 20 (mg / l), but the water after rot and fermentation was 2 (mg / l). Regarding phosphoric acid, raw water was 0.6 (mg / l), but the water after rot and fermentation was 0.35 (mg / l). In this way, the concentration of nitrous acid, nitric acid and phosphoric acid in the raw water could be greatly reduced by rot and fermentation treatment.

  In addition, bamboo charcoal in which the opportunistic bacteria were infested in the micropores in Example 1 and a microorganism culture solution obtained by mixing a useful microorganism group (EM bacteria) in the water of the river and culturing for one week were stirred and mixed. And when this mixed liquid (septic, fermentation processing agent) was thrown into the said river where sludge exists, sludge disappeared, water became transparent, and a bad smell was no longer felt. In this way, it was possible to purify dirty rivers with sludge.

It should be noted that the above embodiment can be modified as follows.
In addition to bamboo charcoal or charcoal, activated carbon, diatomaceous earth, or the like can be blended as a porous material in the rot and fermentation treatment agent.

  In performing the septic treatment and the fermentation treatment, it is possible to remove oxygen in the atmosphere and perform it in an anaerobic atmosphere or in an inert gas atmosphere to improve the treatment efficiency of the septic treatment and the fermentation treatment.

-As a fermentation type microorganism, the anaerobic fermentation type microorganism may be contained in the anaerobic fermentation type microorganism.
Furthermore, the technical idea grasped from the embodiment will be described below.

The method for rot and fermentation treatment of an organic material according to any one of claims 1 to 3 , wherein the rot treatment and the fermentation treatment are performed with stirring. According to this method, in addition to the effects of the invention according to any one of claims 1 to 3 , the rot treatment and the fermentation treatment can be performed more efficiently.

· The organic object to be processed, rot organic treatment object as claimed in any one of claims 3, characterized in that the waste fresh food, fermentation treatment method. According to this method, in addition to the effects of the invention according to any one of claims 1 to 3 , the organic matter to be spoiled and the fermentation treatment method can be applied to the waste of the most familiar fresh food. .

- the bamboo charcoal spoilage bacteria were grown in, rot, fermentation treatment agent of an organic object to be processed according to claim 4, characterized in that a mixture of a fermentation system microorganism thereto. When comprised in this way, in addition to the effect of the invention which concerns on Claim 4 , the decay of an organic to-be-processed object and a fermentation processing agent can be prepared easily.

The rot and fermentation treatment agent according to claim 4 , wherein the rot fungus grows in the fine pores of porous bamboo charcoal. In this case, in addition to the effect of the invention according to claim 4 , the effect of spoilage bacteria can be remarkably improved in the fine pores of bamboo charcoal having a large surface area.

The spoilage and fermentation of an organic material to be treated according to claim 4 , wherein the spoilage fungus is an opportunistic fungus that grows in the fine pores of porous bamboo charcoal by leaving bamboo charcoal in a fine powder. Processing agent. In this case, in addition to the effect of the invention according to claim 4 , the spoilage bacteria can be held in the bamboo charcoal by a simple operation.

Claims (4)

Rotating and fermenting by mixing a rot containing a microorganism that exhibits the function of fermenting and decomposing rot bacteria and rot products into bamboo charcoal, and performing a rot treatment and a fermentation treatment by mixing the organic treatment material, the organic treatment material lactic acid, photosynthetic bacteria, yeast, rotting organic treatment object, which comprises using a microorganism group containing actinomycetes and filamentous fungi, fermentation treatment method upon, as a pre-Symbol microorganisms degrade. The method for rot and fermentation of an organic material to be treated according to claim 1, wherein the rot bacteria are grown in the fine pores of porous bamboo charcoal. The said rot bacteria are opportunistic bacteria which proliferate in the micropores of porous bamboo charcoal by leaving bamboo charcoal as a fine powder, and the rot and fermentation treatment of the organic material to be treated according to claim 2 Way . The rot of the organic material to be treated according to claim 1, the rot used in the fermentation treatment method, and a fermentation treatment agent, wherein the bamboo charcoal contains a microorganism exhibiting a function of fermenting and decomposing the rot bacteria and the rot product. An organic processing material spoilage and fermentation treatment agent characterized by using a group of microorganisms containing lactic acid bacteria, photosynthetic bacteria, yeasts, radioactive bacteria and filamentous fungi as the microorganisms.