JP3728738B1 - Rumen bypass for ruminants - Google Patents

Abstract

【Task】
The biologically active substance is stably protected in the rumen of ruminants inhabiting microorganisms, and is efficiently released in the lower digestive tract after the fourth stomach.
[Solution]
The biologically active substance is selected from hardened animal / vegetable oils and fats, linear or branched saturated or unsaturated aliphatic monocarboxylic acids having 12 to 22 carbon atoms, aliphatic esters, waxes and waxes. At least one coating agent and lecithin and propionic acid or a salt thereof, sorbic acid or a salt thereof, benzoic acid or a salt thereof, dehydroacetic acid or a salt thereof, paraoxybenzoic acid esters, imazalyl, thiabendazole, orthophenylphenol, ortho A rumen bypass agent for ruminants, which is coated with at least one preservative selected from sodium phenylphenol and diphenyl.
[Selection figure] None

Description

  The present invention belongs to a feed additive composition for ruminants. More specifically, rumen bypass for ruminant animals, coated with a coating composition that stably protects the biologically active substance in the rumen rumen and releases it in the lower gastrointestinal tract after the fourth stomach. It relates to the agent.

  Ruminants such as cows have a plurality of stomachs unlike monogastric animals such as humans, and a large number of microorganisms inhabit the first stomach. Therefore, even when an untreated biologically active substance is orally administered to ruminants, most of them are decomposed by microorganisms and are not effectively used.

Numerous proposals have been made for ruminant rumen bypass coating compositions that provide stable protection of the biologically active substance in the rumen rumen and release it in the lower gastrointestinal tract after the fourth stomach. ing. However, at present, there is no rumen bypass agent that has sufficient protection in the first stomach and release in the lower digestive tract after the fourth stomach. This is considered to be due to the fact that the method for evaluating the ruminant protection in the rumen and the structure of the rumen bypass agent having a particle size or a coating layer have not been sufficiently studied.

  In Patent Document 1 or Patent Document 2 and the like, the protection of biologically active substances in the rumen is evaluated with simulated first gastric fluid whose pH and liquid temperature are adjusted. The influence of certain microorganisms that live in the rumen has not been studied. Actually, the commercially available rumen bypass agent had a high protective property in the simulated first gastric juice, but as a result of evaluation using the first gastric fluid of ruminants inhabiting microorganisms, it was extremely protective. Was damaged. The cause of this is thought to be due to the influence of microorganisms that live in large numbers in the rumen of ruminants. Therefore, it was necessary to coat the biologically active substance with a coating composition capable of suppressing the influence of microorganisms that live in the rumen of the ruminant.

Moreover, in patent document 3, although the protective property of a rumen bypass agent is evaluated with the 1st gastric fluid which microbes inhabit, since a particle size is 4-15 mm large, it is easy to disintegrate by chewing, Furthermore, this rumen bypass agent is Due to the double coating, there is a drawback that the protective effect is extremely reduced when the coating on the core surface layer is destroyed by rubbing mastication or the like.

In other words, the commercially available rumen bypass agent is not sufficiently satisfactory in the protection of biologically active substances in the rumen where microorganisms live. This is due to the influence of microorganisms that live in the rumen, which are unique to ruminants, and it was necessary to study the protection of the rumen in consideration of the presence of these microorganisms. In addition, the microorganisms that live in the rumen of ruminants are necessary for maintaining the lives of ruminants and can be nutrients such as proteins. Instead, it was necessary to develop rumen bypass agents for ruminants that were coated with a coating composition capable of protecting biologically active substances.

When a cow farmer orally administers a biologically active substance that is effective for cattle, the biologically active substance is stably protected even in the first stomach where many microorganisms live, and the lower part after the fourth stomach We are keen to supply rumen bypass agents for ruminants that are excellent in release in the digestive tract and are inexpensive.
JP-A-5-23114 JP-A-9-187228 Japanese Patent Laid-Open No. 2000-60440

The problem to be solved by the present invention is that the biologically active substance is stably protected in the rumen of ruminants inhabiting microorganisms, is efficiently released in the lower digestive tract after the fourth stomach, and low The object is to obtain a lumen bypass agent in a cost-manufacturable manner.

As a result of diligent efforts to achieve the above-mentioned object, the present inventors have made biologically active substances A) hardened animal fats and oils, hardened vegetable fats and oils, linear or branched having 12 to 22 carbon atoms. At least one coating agent selected from saturated saturated or unsaturated aliphatic monocarboxylic acids, fatty acid esters, waxes and waxes, B) lecithin and C) propionic acid or a salt thereof, sorbic acid or a salt thereof, benzoic acid Or coating with a coating composition comprising at least one preservative selected from salts thereof, dehydroacetic acid or salts thereof, paraoxybenzoates, imazalyl, thiabendazole, orthophenylphenol, sodium orthophenylphenol and diphenyl This can improve the rumen's rumen protection (lumen bypass rate). The finding has led to the completion of the present invention.

The preservative used in the present invention suppresses the growth of microorganisms such as fungi and bacteria, or has an antibacterial action. By containing this in a rumen bypass agent, biological activity by microorganisms living in the rumen It is considered that the decomposition of the substance and the penetration of the rumen bypass agent into the vicinity of the surface are minimized, and as a result, the disintegration is prevented. In addition, when the preservative is added in a large amount, it may affect the growth and growth of microorganisms. On the other hand, if it is too small, the effect cannot be expected. Therefore, the content of the preservative is preferably 0.01 to 2.0% by weight of the whole rumen bypass agent. Preferably, it is 0.1 to 1.0% by weight.

The production method is selected from hardened animal fats and oils, hardened vegetable fats and oils, linear or branched saturated or unsaturated aliphatic monocarboxylic acids having 12 to 22 carbon atoms, fatty acid esters, waxes and waxes. A granulation method in which at least one coating agent and lecithin are melt-mixed, and a spray melt in which a biologically active substance and a preservative are dispersed and / or dissolved in the melt mixture is sprayed into the air It is. This manufacturing method can obtain a spherical lumen bypass agent only by the granulating step, and can be manufactured at low cost.

Regarding the point of releasing the biologically active substance in the lower digestive tract after the fourth stomach, it is preferable to add lecithin to the rumen bypass agent. Furthermore, by containing stearic acid, it is possible to obtain a rumen bypass agent having excellent protection in the first stomach and excellent release from the fourth stomach.

Regarding the particle diameter of the biologically active substance, it is desirable to use a fine powder because the smaller the biological active substance existing near the surface of the spherical lumen bypass agent, the easier it is to coat.

As a means for obtaining a fine powder of a biologically active substance, there is a method of pulverizing with a pin mill, a ball mill, a jet mill or the like, and a good lumen bypass agent can be obtained by making the average particle size 150 μm or less. On the other hand, when a biologically active substance finely pulverized to an average particle size of 1 μm or less is dispersed in a molten mixture, it is difficult to inject due to an increase in viscosity of the injection melt. It is necessary to lower the content rate considerably. Therefore, it is necessary to adjust the average particle size of the biologically active substance to 1 to 150 μm. Preferably, 10-100 micrometers is desirable.

That is, the present invention relates to (1): particles in which a biologically active substance is coated with a coating composition comprising the following A), B) and C) , wherein the particles contain 0.01 to 2.0% by weight of C). A rumen bypass agent for ruminants characterized by this. A) at least selected from hardened animal fats, hardened vegetable fats and oils, linear or branched saturated or unsaturated aliphatic monocarboxylic acids having 12 to 22 carbon atoms, fatty acid esters, waxes and waxes One coating agent, B) lecithin, C) propionic acid or a salt thereof, sorbic acid or a salt thereof, benzoic acid or a salt thereof, dehydroacetic acid or a salt thereof, paraoxybenzoic acid esters, imazalyl, thiabendazole, orthophenylphenol, The rumen bypass for ruminants according to claim 1, characterized in that at least one preservative selected from sodium orthophenylphenol and diphenyl, (2): the preservative of C) is propionic acid or a salt thereof. Agent (3): the biologically active substance contains at least lysine hydrochloride The rumen bypass agent for ruminants according to any one of claims 1 and 2, (4): a linear or branched saturated or unsaturated fat in which the coating agent of A) has at least 12 to 22 carbon atoms. A rumen bypass agent for ruminants according to any one of claims 1 to 3, which contains an aliphatic monocarboxylic acid, (5): linear or branched saturated having 12 to 22 carbon atoms The rumen bypass agent for ruminants according to any one of claims 1 to 4, wherein the unsaturated aliphatic monocarboxylic acid is stearic acid, (6): coating composition of biologically active substance The ruminant according to any one of claims 1 to 5, wherein the ruminant is obtained by injecting and granulating an injection melt, which is dispersed and / or dissolved in a molten mixture of products, into the air. Lumen bypass agent (7): feed containing ruminant lumen bypassing agent according to any one of claims 1 to 6, relates.

As explained above, the biologically active substance is: A) hardened animal oil, hardened vegetable oil, straight chain or branched saturated or unsaturated aliphatic monocarboxylic acid having 12 to 22 carbon atoms At least one coating agent selected from acids, fatty acid esters, waxes and waxes, B) lecithin and C) propionic acid or salts thereof, sorbic acid or salts thereof, benzoic acid or salts thereof, dehydroacetic acid or salts thereof, paraoxy Protecting ruminant rumen by coating with a coating composition comprising at least one preservative selected from benzoates, imazalyl, thiabendazole, orthophenylphenol, orthophenylphenol sodium and diphenyl , Providing a lumen bypass agent excellent in release in the lower digestive tract after the fourth stomach, Significance on the work is very large.

In the present invention, as the biologically active substance, at least one kind or a mixture of two or more kinds selected from amino acids, vitamins, enzymes, carbohydrates, natural products and pharmaceuticals can be used.

Specifically, amino acids include aminoacetic acid, alanine, arginine, lysine, sodium glutamate, methionine, tryptophan, threonine, valine, betaine, taurine, etc .; vitamins such as vitamin A and vitamin D , Vitamin E, vitamin C, vitamin B1, vitamin B2, vitamin B6, vitamin B12, choline chloride, calcium pantothenate, nicotinic acid, nicotinic acid amide, biotin, folic acid, etc .; enzymes include protease, amylase, lipase, mixed enzyme Carbohydrates include starch, sucrose, and glucose; natural products such as fish meal, seaweed powder, blood meal, cereal meal, and bile powder; pharmaceuticals include antibiotics and hormones; System, Te Rasaikurin system, penicillins, macrolides, such as lincomycin system. Examples of the hormone drug estrogen, stilbestrol and hexestrol, and the like.

In the coating agent, as the hardened animal fats and oils, beef tallow, pork fat and the like, and as the hardened vegetable fats and oils, palm hardened oil, soybean hardened oil, rapeseed hardened oil, castor hardened oil and the like have 12 to 22 carbon atoms. Examples of the linear or branched saturated or unsaturated aliphatic monocarboxylic acid include lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, etc., and fatty acid esters include glycerin fatty acid ester, sucrose Examples of waxes that can be used include fatty acid esters such as carnauba wax and beeswax, and waxes that can be used include natural wax, synthetic wax, and paraffin wax.

In the preservative, propionic acid or its salt is propionic acid, calcium propionate, sodium propionate, sorbic acid or its salt is sorbic acid, potassium sorbate, benzoic acid or its salt is benzoic acid, benzoic acid Examples of sodium acid, dehydroacetic acid or salts thereof include dehydroacetic acid, sodium dehydroacetate, and paraoxybenzoic acid esters such as isobutyl paraoxybenzoate, isopropyl paraoxybenzoate, ethyl paraoxybenzoate, butyl paraoxybenzoate, and propyl paraoxybenzoate Can be used.

If necessary, the specific gravity may be adjusted by using calcium carbonate, calcium phosphate, talc or the like as a specific gravity adjusting agent.

Further, the rumen bypass agent obtained by spray granulation can be uniformly mixed with raw materials such as defatted rice bran, wheat flour, dried okara, corn and / or fish meal with a mixer such as a ribbon mixer to obtain a feed.

The present invention will be described in further detail. The present invention is not limited in any way by the following examples.

Evaluation as a lumen bypass agent of the present invention was performed by the following method.
(Evaluation of protective property of the first stomach) The gastric juice is obtained by sucking the first gastric fluid from the cow's mouth through the tube from the middle layer of the first stomach to the vicinity of the lower layer. Immediately after obtaining the first gastric juice, the rumen bypass agent is immersed in 900 mL of the first gastric juice and stirred at 40 ° C. for 16 hours. After stirring, all the rumen bypass agents used in this test were collected, and the residual rate of biologically active substances (lumen bypass rate) was measured and evaluated.

(Evaluation of dissolution property of the fourth stomach) The rumen bypass agent is immersed in 900 mL of the first gastric juice collected by the above method and stirred at 40 ° C. for 16 hours. After stirring, all of the rumen bypass agent used in this test was collected and immersed in 900 mL of Clark-Lubs buffer corresponding to the fourth gastric juice. This was stirred for 2 hours at 37 ° C., and the biologically active substance eluted was measured and evaluated. Clark-Lubs buffer: A buffer solution in which 3.73 g of potassium chloride and 2.1 mL of hydrochloric acid are dissolved in water.

(Evaluation of dissolution property of the small intestine) After measuring the dissolution rate of the fourth gastric juice, all the rumen bypass agents used in this test were collected and immersed in 900 mL of a buffer solution corresponding to the small intestinal fluid. This was stirred at 37 ° C. for 7 hours, and the biologically active substance eluted was measured and evaluated.

1626 g of rapeseed oil, 90 g of lecithin, and 150 g of stearic acid were melt-mixed and kept at 80 ° C., and then 1125 g of lysine hydrochloride (average particle size of about 50 μm) adjusted in particle size and calcium propionate (preservative) 9 g) was added and dispersed uniformly to obtain a melt for injection. The melt for spraying was sprayed into the air to obtain a lumen bypass agent solidified into a spherical shape of 0.5 to 2.0 mm.

1626 g of rapeseed oil, 90 g of lecithin and 150 g of stearic acid were melt-mixed and kept at 80 ° C., and then 1125 g of lysine hydrochloride (average particle size of about 50 μm) adjusted in particle size and sodium propionate (preservative) Agent) 9 g was added and dispersed uniformly, and the same production as in Example 1 was carried out to obtain a lumen bypass agent.

1517 g of hydrogenated palm oil, 90 g of lecithin, and 150 g of stearic acid were melt-mixed and kept at 80 ° C., and then 1125 g of lysine hydrochloride (average particle size of about 50 μm) whose particle size was adjusted to this molten mixture, calcium propionate (preservative) Agent) 18 g and calcium carbonate 100 g were added and dispersed uniformly, and the same production as in Example 1 was carried out to obtain a rumen bypass agent.

1608 g of rapeseed hydrogenated oil, 90 g of lecithin and 150 g of stearic acid were melt-mixed and kept at 80 ° C., and then 1125 g of lysine hydrochloride (average particle size of about 50 μm) adjusted in particle size and calcium propionate (preservative) Agent) 27 g was added and dispersed uniformly, and the same procedure as in Example 1 was carried out to obtain a lumen bypass agent.

1517 g of hardened rapeseed oil, 90 g of lecithin and 150 g of stearic acid were melt-mixed and kept at 80 ° C., and then 1125 g of taurine (average particle size of about 50 μm) whose particle size was adjusted to this molten mixture, calcium propionate (preservative) 18 g and 100 g of calcium carbonate were added and dispersed uniformly and produced in the same manner as in Example 1 to obtain a rumen bypass agent.

(Comparative Example 1)
1635 g of rapeseed oil, 90 g of lecithin and 150 g of stearic acid were melt-mixed and kept at 80 ° C., and then 1125 g of lysine hydrochloride (average particle size of about 50 μm) with adjusted particle size was added to this melt and dispersed uniformly. And it manufactured like Example 1, and obtained the rumen bypass agent.

(Comparative Example 2)
After 1535 g of hardened palm oil, 90 g of lecithin and 150 g of stearic acid were melt-mixed and kept at 80 ° C., 1125 g of lysine hydrochloride (average particle size of about 50 μm) whose particle size was adjusted and 100 g of calcium carbonate were added to the melt mixture. Were uniformly dispersed and produced in the same manner as in Example 1 to obtain a rumen bypass agent.

(Comparative Example 3)
1716 g of rapeseed oil and 150 g of stearic acid were melt-mixed and kept at 80 ° C., and then 1125 g of lysine hydrochloride (average particle size of about 50 μm) with adjusted particle size was added to this melt and 9 g of calcium propionate (preservative). Was added and dispersed uniformly to produce a lumen bypass agent in the same manner as in Example 1.

(Comparative Example 4)
1626 g of rapeseed hydrogenated oil, 90 g of lecithin and 150 g of stearic acid were melt-mixed and kept at 80 ° C., then 1125 g of lysine hydrochloride (average particle size of about 200 μm) adjusted in particle size was added to this molten mixture, calcium propionate (preservative) Agent) 9 g was added and dispersed uniformly, and the same production as in Example 1 was carried out to obtain a lumen bypass agent.

(Comparative Example 5)
1535 g of rapeseed oil, 90 g of lecithin and 150 g of stearic acid were melt-mixed and kept at 80 ° C., and then 1125 g of taurine (average particle size of about 50 μm) with adjusted particle size and 100 g of calcium carbonate were added uniformly to this melted mixture. And was produced in the same manner as in Example 1 to obtain a rumen bypass agent.

Examples are shown in Table 1 and Comparative Examples are shown in Table 2. From the examples and comparative examples, the rumen bypass agent containing a preservative showed good results in ruminant protection in the rumen (lumen bypass rate).

As described above, the biologically active substance is divided into A) hardened animal oil and fat, hardened vegetable oil and fat, straight chain or branched saturated or unsaturated aliphatic monocarboxylic acid having 12 to 22 carbon atoms. At least one coating agent selected from fatty acid esters, waxes and waxes, B) lecithin and C) propionic acid or a salt thereof, sorbic acid or a salt thereof, benzoic acid or a salt thereof, dehydroacetic acid or a salt thereof, paraoxybenzoic acid A rumen bypass agent for ruminants characterized by being coated with a coating composition comprising at least one preservative selected from acid esters, imazalyl, thiabendazole, orthophenylphenol, sodium orthophenylphenol and diphenyl. Protection (high lumen bypass rate) and release in the lower digestive tract after the 4th stomach It has been found that is also excellent sex.

Claims (7)

Rumen for ruminant animals characterized in that a biologically active substance is coated with a coating composition comprising the following A), B) and C) , wherein the particle contains 0.01 to 2.0% by weight of C) Bypass agent. A) at least selected from hardened animal fats, hardened vegetable fats and oils, linear or branched saturated or unsaturated aliphatic monocarboxylic acids having 12 to 22 carbon atoms, fatty acid esters, waxes and waxes One coating agent, B) lecithin, C) propionic acid or a salt thereof, sorbic acid or a salt thereof, benzoic acid or a salt thereof, dehydroacetic acid or a salt thereof, paraoxybenzoic acid esters, imazalyl, thiabendazole, orthophenylphenol, At least one preservative selected from sodium orthophenylphenol and diphenyl. The rumen bypass agent for ruminants according to claim 1, wherein the antiseptic of C) is propionic acid or a salt thereof. The rumen bypass agent for ruminants according to claim 1 or 2, wherein the biologically active substance contains at least lysine hydrochloride. The coating agent of A) contains a linear or branched saturated or unsaturated aliphatic monocarboxylic acid having at least 12 to 22 carbon atoms. The rumen bypass agent for ruminants as described. The rumen for ruminants according to any one of claims 1 to 4, wherein the linear or branched saturated or unsaturated aliphatic monocarboxylic acid having 12 to 22 carbon atoms is stearic acid. Bypass agent. Claims 1 to Claims obtained by granulating a jetting melt in which a biologically active substance is dispersed and / or dissolved in a melted mixture of a coating composition into air. 5. A rumen bypass agent for ruminants according to any one of 5 above. Feed containing the rumen bypass agent for ruminants according to any one of claims 1 to 6.