JP3449967B2 - External preparation for skin - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an external preparation for skin in which the effects of preventing and improving skin roughness and skin aging are synergistically improved. More specifically, an anti-aging treatment comprising a combination of one or more selected from the extract of Kacon and an extract fraction thereof, and one or two or more selected from an extract of Waremoko and an extract fraction thereof. , For improving skin external preparation .

[0002]

2. Description of the Related Art Aging, UV exposure, oxidative stress due to reactive oxygen species generated in skin tissue, contact with drugs and various allergens, etc. may cause an inflammatory reaction and aging of the skin. Are known. In the field of external preparations for skin, many physiologically active ingredients such as active oxygen species scavengers, anti-inflammatory agents and anti-allergic agents have been searched and studied in order to prevent such skin inflammation and aging.
In recent years, whether it reflects the natural and plant orientation of consumers,
There is a growing tendency to seek such components from plants.

However, among the above-mentioned components of plant origin which have already been reported, since the activity is low, it takes a considerably high concentration to obtain a sufficient action and effect when incorporated into an external preparation for skin, and it is stable. Stability and safety when combined with external preparations for skin, as well as effects that may cause unpleasant color and odor to external preparations for skin. The current situation is that there are few things that can be satisfied in all aspects. In addition, various factors such as inflammatory reaction and aging of skin are complicatedly involved and progressed, and therefore, sufficient effects were not obtained even if a substance acting only in a part of the above-mentioned reaction was used.

[0004]

Therefore, in the present invention, it is possible to obtain a skin external preparation which suppresses complicated inflammatory reaction of skin and the like, prevents skin roughening and skin aging, and has synergistically improved effects. Intended.

[0005]

It made various studies to solve the above problems [SUMMARY OF INVENTION The present inventors have Kakkonekisu (Pueraria
e Radix ) and extract fractions thereof have a high stimulatory effect on collagen production by fibroblasts, and one or more selected from these, and Waremoko ( Sanguisorba officinalis L.). By combining one or two or more selected from the extract and the extract fraction of the above in an external preparation for the skin, a good anti-inflammatory effect can be obtained, and the rough skin and skin aging can be prevented and improved. The present invention has been completed by finding that a synergistic improvement in effect can be obtained.

[0006] In particular, when the curcuma extract and the extract fraction having a high content of 4 ', 7-dihydroxyisoflavone (daidzein) are used, good effects are obtained, and
You may use together 4 ', 7- dihydroxy isoflavone itself and an extract of Pleurotus cornucopiae.

The cucumber extract has a mucopolysaccharide fragmentation-inhibiting effect, active oxygen scavenging effect, and antioxidative effect (JP-A-6-
24937), a fat synthesis promoting action (JP-A-11-199).
499), and dermal papilla activation effect (JP-A-11-24082).
3) is disclosed, and a hyaluronic acid production promoting action is also reported (Proceedings of the 120th Annual Meeting of the Pharmaceutical Society of Japan, 2, 58).
Page, 2000), but nothing about the action of promoting the production of type I collagen. Furthermore, it has been reported that a metabolite of 7-isopropoxy isoflavone has a type I collagen synthesis promoting action (Calcified Tissue Int.
ernational 55 (5) 356-362 (1994)) is known to have an anti-acetylcholine action with respect to 4 ', 7-dihydroxyisoflavone contained in cucumber extract, but no such report has been made so far.

[0008] Further, as for the oleander extract, an action of inhibiting collagenase in a dry powdery water extract is disclosed (JP-A-7-196526), and other hydroxyl radicals or singlet oxygen scavenging action (JP-A-7-13321).
6), chemical mediator release inhibitory effect
0-36276), improvement of skin strength, morphological improvement of keratinocytes (JP-A-11-29460), dermal collagen fiber bundle improvement (JP-A-11-12122), elastase inhibitory activity (JP2969451) and the like. ing. However, the effect of the present invention obtained by using the extract of Kacon and the extracted fraction thereof in combination with the extract or extracted fraction of Corydalis has never been suggested at all.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION The parentheses ( Puerariae Radix ) used in the present invention are roots excluding the pericardium of Kudzu ( Pueraria lobata Ohwi), which is a deciduous Fujimoto belonging to the leguminous family ( Leguminosae ). Also, square brackets, plate brackets and powder brackets produced in China can be used.

[0010] In addition, Sanguisorba officina
lis L.) is a perennial herb belonging to Rosaceae ,
Although various parts such as leaves, stems, flowers, and roots can be used, it is preferable to use root parts.

[0011] Cuckons ( Puerariae Radix ) and Waremoko ( Sanguisorba officinalis L.) may be used for extraction as they are, but in consideration of extraction efficiency, they are finely chopped, dried,
It is preferable to perform extraction after performing processing such as crushing.
The extraction is performed by immersing it in an extraction solvent. It may be stirred or homogenized in an extraction solvent in order to improve the extraction efficiency. As the extraction temperature, it is appropriate to set the temperature to about 5 ° C. to a temperature not higher than the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is suitable to be about 4 hours to 14 days.

As the extraction solvent, in addition to water, methanol,
Lower alcohols such as ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, ethers such as ethyl ether and propyl ether, esters such as ethyl acetate and butyl acetate. It is possible to use polar organic solvents such as acetone, ketones such as ethyl methyl ketone, etc., and one kind or two or more kinds are selected from these and used. Alternatively, physiological saline, phosphate buffer, phosphate buffered saline, etc. may be used.

Parentheses (Puerariae Radix) And Valemo
Ko (Sanguisorba officinalis L.) depending on the above solvent
The extract is contained in the skin external preparation according to the present invention as it is
However, it is possible to concentrate and dry the product in water or polar
It may be redissolved in a solvent or its physiological effects may be impaired.
Purification treatment such as decolorization, deodorization, and desalting was performed within the range not found.
And perform fractionation processing such as column chromatography.
It may be used after it is used. Especially the parentheses (Puerariae Radi
x) For the extract, after adsorbing it on a synthetic adsorption resin,
When eluted sequentially with water / ethanol mixed solvent, 50% ~
The fraction eluted with a 99.5% ethanol aqueous solution
The highest content of 4 ', 7-dihydroxyisoflavone
Most preferably used from the viewpoint of promoting the production of lagen
It As a synthetic adsorption resin, adsorption made of styrene polymer
Resin MCI gel HP-20 (Mitsubishi)
Chemical Industry Co., Ltd.) are preferably used. Parentheses
And the processed product and fractionated product thereof
After each treatment and fractionation, freeze-dry and dissolve in a solvent before use.
It can also be used. In addition, vesicles such as liposomes and
It can also be used by being encapsulated in a microcapsule or the like.

Further, in the present invention, purified 4 ′, 7-dihydroxyisoflavone may be used in place of the above extract of curcuma or the fraction thereof.

In the present invention, the above-mentioned parentheses ( Puerar
iae Radix ) and one or more kinds of extract fractions or 4 ', 7-dihydroxyisoflavone and one or two kinds of extract and extract fractions of Waremoko ( Sanguisorba officinalis L.) The above is added to the skin external preparation base. The external preparation for skin according to the present invention includes lotions, emulsions, gels, creams, ointments, powders, granules, etc.
It can be provided in various dosage forms. In addition, skin lotion, emulsion, cream, beauty essence, skin cosmetics such as packs, makeup base lotion, foundation cosmetics such as makeup base cream, foundations, eye lotions for each formulation such as emulsion, oil, and solid. It can be provided as makeup cosmetics such as colors and cheek colors.

[0016] Note that the external skin preparation according to the present invention, Kakkonekisu (Puerariae Radix) extract or the like and Burnet (Sangu
isorba officinalis L.) extract, etc., as well as general pharmaceuticals and cosmetics such as oily ingredients, surfactants, humectants, pigments, UV absorbers, antioxidants, fragrances, antibacterial and antifungal agents, etc. Raw materials and physiologically active ingredients such as skin cell activating agents, anti-inflammatory agents, and whitening agents can be contained.

[0017]

EXAMPLES The features of the present invention will be described in detail with reference to Examples.

First, the preparation of the extract and extract fraction of Kacon ( Puerariae Radix ) and the extract and extract fraction of Sanguisorba officinalis L. contained in the external preparation for skin according to the present invention will be described.

[Kakkon Extract 1] 200 g of dried powder of Kakkon was immersed in 1 liter of ethanol and allowed to stand at 20 ° C. for 7 days for extraction, and the extract was filtered, recovered, concentrated and dried. It was lyophilized to give the title Kacon extract.

[Kakkon Extract 2] 500 g of Kakkon was cut into small pieces, extracted with stirring in 2 liters of ethanol at 20 ° C. for 3 days, and the extract was filtered, recovered, concentrated and dried.
It was dissolved in 1 liter of glycerin to obtain the title Kacon extract.

[Kakkon extract 3] 250 g of kakkon extract was dried and pulverized, immersed in 2 liters of 50% by volume aqueous ethanol at 20 ° C. for 7 days, and then filtered to collect the filtrate to obtain kakkon extract 3. did.

[Kakkon Extraction Fractions 1 to 4] 500 g of Kakon ( Puerariae Radix ) was dried and pulverized, immersed in 2 liters of a 50% by volume ethanol aqueous solution, and kept at 20 ° C.
After leaving still for 7 days for extraction, it is filtered to collect the filtrate,
Then concentrate, dry to dryness. This dry powder 3
Dissolve 6.0 g in 500 ml of ethanol, add 1500 ml of purified water, and add 600 ml of DIAION M.
CI gel HP-20 (manufactured by Mitsubishi Kagaku Co., Ltd.) was added and stirred for 1 hour, and then the resin was recovered by filtration, packed in a column, and eluted sequentially with a mixed solvent of water and ethanol in a stepwise manner. . At that time, 50% by volume aqueous ethanol solution, 70
Fractions eluted with a volume% ethanol aqueous solution, a 90% ethanol aqueous solution and a 99.5 volume% ethanol aqueous solution are collected, and each is lyophilized to give the title extract fractions 1 to 4
And

[Walemkou Extract 1] Waremoko ( Sang
350 g of roots of uisorba officinalis L.) are dried and crushed, immersed in 1.5 liter of 50% by volume ethanol aqueous solution at 20 ° C. for 7 days, and then filtered to collect a filtrate,
This was designated as the title extract 1.

[Walemkou Extract 2] Waremoko ( Sang
250 g root of uisorba officinalis L.)
3 in 2 liters of 3-butylene glycol at 20 ° C
It was extracted with stirring for one day. The filtrate was collected by filtration to obtain the title extract 2.

[Walemkou Extract 3] Waremoko ( Sang
uisorba officinalis L.) leaves and roots totaling 450 g,
The extract was homogenized in 2.5 liters of phosphate-buffered saline (pH = 7.2) and centrifuged to collect the supernatant, which was designated as Extract 3.

[Walemkou Extract 4] Waremoko ( Sang
uisorba officinalis L.) root (500 g) is dried, crushed, immersed in 2.5 liters of ethanol, and dried at 15 ° C for 1 hour.
The mixture was left standing for 0 days for extraction, and the extract was collected by filtration, concentrated, dried and freeze-dried to obtain the title extract 4.

[Waremoko extract fractions 1 and 2] 500 g of roots of Warimokou ( Sanguisorba officinalis L.) were dried, crushed and immersed in 2.5 liters of ethanol,
After allowing to stand at 20 ° C. for 7 days for extraction, the filtrate is collected by filtration, then concentrated, dried and freeze-dried. This dry powder was dissolved in 500 ml of ethanol and purified water 15
After adding 00 ml and adding 600 ml of DIAION MCI gel HP-20 (manufactured by Mitsubishi Kagaku Co., Ltd.) and stirring for 1 hour, the resin was recovered by filtration and packed in a column, which was used as a mixed solvent of water and ethanol. And then eluted stepwise. At that time, fractions eluted with a 30% by volume aqueous ethanol solution and a 50% by volume aqueous ethanol solution were collected and freeze-dried to obtain the title extraction fractions 1 and 2.

Next, the formulations of the examples of the external preparation for skin according to the present invention will be shown.

[Example 1] Lotion agent (1) Ethanol 20.00 (wt%) (2) Polyoxyethylene (60 E.O.) hydrogenated castor oil 1.00 (3) Kacon extract 10 0.02 ( 4) Waremoko extract 1 0.20 (5) Methyl paraoxybenzoate 0.10 (6) Dipropylene glycol 5.00 (7) 1,3-Butylene glycol 10.00 (8) Purified water 63.68 Production method: (2) to (5) are added to (1) and dissolved to obtain an alcohol phase. On the other hand, (6) and (7) are sequentially dissolved in (8) to form an aqueous phase. The alcohol phase is added to the aqueous phase, and the mixture is stirred and mixed.

Example 2 Emulsion (1) Cetanol 1.00 (wt%) (2) Beeswax 0.50 (3) Vaseline 2.00 (4) Squalane 6.00 (5) Dimethylpolysiloxane 2.00 (6) Polyoxyethylene (20 E.O.) sorbitan 1.00 Monostearic acid ester (7) Glyceryl monostearic acid ester 1.00 (8) Glycerin 4.00 (9) 1,3-Butylene glycol 4.00 (10) Methyl paraoxybenzoate 0.10 (11) Purified water 63.23 (12) Carboxyvinyl polymer 10.00 (1.0% by weight aqueous solution) (13) Potassium hydroxide 0.10 (14) Ethanol 5. 00 (15) Kacon extract fraction 1 0.02 (16) Waremoko extract 4 0.05 Production method: The oil phase components of (1) to (7) are mixed, heated and dissolved to obtain 75
℃. On the other hand, the water phase components (8) to (11) are mixed and dissolved to 75 ° C. After adding the oil phase to this and pre-emulsifying, add (12) and homogenize with a homomixer, then add (13) to thicken and cool, and cool at 40 ° C (15). ，
Dissolve (16) in (14) and add and mix.

[Example 3] Oil-in-water cream (1) Beeswax 6.00 (% by weight) (2) Cetanol 5.00 (3) Reduced lanolin 8.00 (4) Squalane 27.50 (5) Glyceryl Fatty acid ester 4.00 (6) Lipophilic glyceryl monostearate 2.00 (7) Polyoxyethylene (20 E.O.) sorbitan 5.00 Monolaurate (8) Propylene glycol 5.00 (9) Paraoxy Methyl benzoate 0.10 (10) Fragment of Kacon extract 2 0.02 (11) Waremoko extract 2 0.25 (12) Purified water 37.13 Process: Add the oil phase components of (1) to (7) Mix and dissolve to 75 ° C. On the other hand, mix and dissolve the water phase components (8) to (12) to 75
Heat to ℃. Next, the oil phase component is added to this aqueous phase component to pre-emulsify it, then homogenize with a homomixer and cool.

Example 4 Gel Agent (1) Dipropylene Glycol 10.00 (wt%) (2) Carboxyvinyl Polymer 0.50 (3) Potassium Hydroxide 0.10 (4) Methyl Paraoxybenzoate 10 (5) Kacon extract fraction 3 0.02 (6) Waremoko extract 4 0.03 (7) Purified water 89.25 Production method: (2) is uniformly dissolved in (7), and then (1) (4) to (6) are dissolved and added to, and then (3) is added to thicken.

[Example 5] Oil-in-water emulsion type ointment (1) White petrolatum 25.00 (% by weight) (2) Stearyl alcohol 25.00 (3) Glycerin 12.00 (4) Sodium lauryl sulfate 1.00 (5) Methyl paraoxybenzoate 0.10 (6) Purified water 36.50 (7) 1.0 (w / v)% 0.15 ethanol solution of Kacon extract fraction 4 (8) Waremoko extract fraction 1.0 (w / v)% 0.25 ethanol solution manufacturing method: The oil phase components (1) to (4) are mixed and heated to uniformly dissolve them, and the mixture is heated to 75 ° C. On the other hand, mix the water phase components of (5) and (6),
Heat to 75 ° C. The oil phase component was gradually added to this water phase component while stirring to emulsify and cool the mixture.
Add (7) and (8) at ℃ and mix.

[Example 6] Liposome agent (1) Glycerin 2.0 (wt%) (2) 1,3-butylene glycol 3.0 (3) Polyoxyethylene (25E.O.) oleyl ether 0.2 (4) Ethanol 10.0 (5) Methyl paraoxybenzoate 0.1 (6) Purified water 79.7 (7) 4 ', 7-Dihydroxyisoflavone, Waremoko 5.0 Extracted fraction 2 encapsulating liposome production method: ( Dissolve (5) in (4), add to (6) together with (1) to (3), mix evenly, and add (7) to this and disperse. In addition, (7) liposomes containing 4 ', 7-dihydroxyisoflavone and Waremoko extract fraction 2 contained 1.0 (w / v)% of 4', 7-dihydroxyisoflavone and Waremoko extract fraction 2. 80 g of soybean lecithin was added to 100 ml of 50% by volume ethanol aqueous solution containing 2.5 (w / v)%, and the temperature was 55 ° C.
And then sonicate to prepare liposomes,
It was recovered by centrifugation and obtained.

[Example 7] Water-in-oil type emollient cream (1) Liquid paraffin 30.00 (wt%) (2) Microcrystalline wax 2.00 (3) Vaseline 5.00 (4) Diglyceryl dioleic acid Ester 5.00 (5) Sodium L-glutamate 1.60 (6) L-Serine 0.40 (7) Propylene glycol 3.00 (8) Methyl paraoxybenzoate 0.10 (9) Kacon extract 20. 02 (10) Waremoko extract 3 0.02 (11) Purified water 52.76 (12) Perfume 0.10 Production method: (5) and (6) were dissolved in a part of (11) to 50 ° C., Gradually add to (4) previously heated to 50 ° C. with stirring. These are mixed in advance and uniformly dispersed in (1) to (3) which are dissolved by heating at 70 ° C. Add (8), (9) to (7)
Dissolved in (10) and added to the rest of (11), heated to 70 ° C., added with stirring, and emulsified with a homomixer. After cooling, (12) is added and mixed at 40 ° C.

[Example 8] Makeup base cream (1) Stearic acid 12.00 (% by weight) (2) Cetanol 2.00 (3) Glyceryl tri-2-ethylhexanoate 2.50 (4) Self-emulsification Type Glyceryl monostearic acid 2.00 Ester (5) Propylene glycol 10.00 (6) Potassium hydroxide 0.30 (7) Methyl paraoxybenzoate 0.10 (8) Purified water 68.43 (9) Titanium oxide 2 0.000 (10) Red iron oxide 0.40 (11) Yellow iron oxide 0.10 (12) Perfume 0.10 (13) Bracon extract 3 0.05 (14) Waremoko extract 1 0.02 Production method: (1) The oil phase components (4) to (4) are mixed and dissolved to reach 75 ° C. On the other hand, the aqueous phase components (5) to (8) are mixed, heated and dissolved, and the pigment components (9) to (11) are added thereto, and the mixture is uniformly dispersed with a homomixer to 75 ° C. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified with a homomixer. After cooling, (12) to (14) are added and mixed at 40 ° C.

Example 9 Milky liquid foundation (1) Stearic acid 2.00 (wt%) (2) Squalane 5.00 (3) Octyldodecyl myristate 5.00 (4) Cetanol 1.00 (5) Decaglyceryl monoisopalmitic acid ester 9.00 (6) 1,3-butyleneglycol 6.00 (7) Potassium hydroxide 0.08 (8) Methyl paraoxybenzoate 0.10 (9) Capcon extract 10 .01 (10) Waremoko extract 4 0.05 (11) Purified water 53.51 (12) Titanium oxide 9.00 (13) Talc 7.40 (14) Red iron oxide 0.50 (15) Yellow iron oxide 1. 10 (16) Black iron oxide 0.10 (17) Perfume 0.15 Production method: The oil phase components (1) to (5) are mixed and dissolved to 75 ° C. On the other hand, the water phase components (6) to (11) are mixed, heated and dissolved,
The pigment components (12) to (16) are added to this and uniformly dispersed by a homomixer to 75 ° C. Then, the above oil phase component is added to this water phase component to uniformly emulsify with a homomixer, and after cooling, (17) is added and mixed at 40 ° C.

Example 10 Hand Cream (1) Cetanol 4.00 (wt%) (2) Vaseline 2.00 (3) Liquid Paraffin 10.00 (4) Glyceryl Monostearate 1.50 (5) Polyoxyethylene (60 E.O.) glyceryl 2.50 isostearic acid ester (6) tocopherol acetate 0.25 (7) glycerin 20.00 (8) methyl paraoxybenzoate 0.10 (9) Kacon extract fraction 1 0.02 (10) Waremoko extract fraction 2 0.02 (11) Purified water 59.61 Production method: Mix and dissolve the oil phase components (1) to (6) to 75 ° C. On the other hand, the aqueous phase components (7) to (11) are mixed, heated and dissolved to 75 ° C. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified with a homomixer and cooled.

Among the examples according to the present invention described above, Examples 1 to 6 were evaluated for the effect of suppressing the formation of skin wrinkles by medium wavelength ultraviolet light (UVB). At that time, in Examples 1 to 6, the Kacon extract or the fraction and the Dianthus communis extract or the fraction, which were respectively blended,
Alternatively, 4 ', 7-dihydroxyisoflavone, and a liposome encapsulating extract of Dioscorea sinensis L. were replaced as shown in Table 1 to give Comparative Examples 1 to 6, and the evaluation was carried out at the same time. Evaluation,
Five hairless mice were treated as one group, and the examples and comparative examples were applied to the back once a day for each group, and 100 mJ
UVB of / cm ² / cycle was irradiated 3 times a week for 20 weeks, the formation of wrinkles on the skin of hairless mice was observed, and scored and evaluated according to the criteria shown in Table 2.
At this time, a group to which only purified water was applied served as a control. The results are shown in Table 3 by calculating the average value of each group and the relationship with the number of UVB irradiation days.

[0040]

[Table 1]

[0041]

[Table 2]

[0042]

[Table 3]

As is clear from Table 3, in the control, the wrinkles formed on the skin reached a moderate depth when the UVB irradiation days exceeded 10 weeks, and deep wrinkles were formed after 20 weeks. Was recognized. Fragment extract 4
No significant wrinkle formation-inhibiting effect was observed in the application group of Comparative Example 5 in which purified water was substituted for both of the extract and Fragmentum vulgaris extract 1 as compared with the control. Comparative Examples 1 to 4 in which purified water was substituted for either the extract or the extract fraction of Kacon and the extract or the extract fraction of Waremoko, and liposomes containing only 4 ', 7-dihydroxyisoflavone were prepared. In the application group containing Comparative Example 6, the formation of slight wrinkles was observed after 20 weeks, and the formation of wrinkles was well suppressed. However, in the application group of Examples, formation of micro wrinkles was observed after 20 weeks in all cases. The effect of suppressing wrinkle formation was significantly improved as compared with the corresponding comparative application group.

Subsequently, use tests were carried out on Examples 1 to 10 of the present invention to evaluate the effect of improving skin aging symptoms. At that time, in Examples 7 to 10, the blended Kacon extract and Dioscorea radix L. extract or extract fraction were replaced as shown in Table 4 to give Comparative Examples 7 to 10, and the above Comparative Examples 1 to 10 were used. It was subjected to a usage test together with Comparative Example 6.

[0045]

[Table 4]

As for the effect of improving skin aging symptoms, fine wrinkle formation and reduction in skin elasticity are noticeable.
20 female panelists in their teens were set as one group, and each of the examples and the comparative examples was blindly used twice a day for 2 months continuously and evaluated. The degree of fine wrinkles is evaluated by visual observation and photography, and the skin elasticity is measured by a cute meter, and the states before and after the use test are compared, respectively, and “improved”, “slightly improved”, and “changed”. The evaluation was made in three grades of "none". The results are shown in Table 5 by the number of panelists who obtained each evaluation.

[0047]

[Table 5]

As is clear from Table 5, in each of the other groups used in Comparative Examples, except for the group used in Comparative Example 5 in which purified water was substituted for both the Kacon extract fraction and the Waremoko extract fraction, no good results were obtained. Although the tendency of improvement of wrinkle and skin elasticity was observed, the number of panelists in which the clear improvement was recognized was significantly increased in the example use group as compared with the corresponding comparative example use group.

Further, with respect to Examples 1 to 10 and Comparative Examples 1 to 10 of the present invention, the effect of improving rough skin symptoms was evaluated. The effect of improving skin roughening symptoms was as follows. Twenty female panelists in their twenties to sixties exhibiting remarkable skin roughening symptoms were treated as one group, and each of the Examples and Comparative Examples was blinded twice a day to each group. It was evaluated by allowing it to be used continuously for a month. The skin condition before and after the use test was evaluated and scored according to the evaluation criteria shown in Table 6, and the average value of 20 persons was calculated and shown in Table 7.

[0050]

[Table 6]

[0051]

[Table 7]

As is clear from Table 7, in all the groups used in the examples of the present invention, remarkable improvement in rough skin was observed, and the skin condition was improved to a good condition after the end of the use test. On the other hand, in the group used in Comparative Example as well, except for the group used in Comparative Example 5, a considerably good improvement in rough skin was observed, but the degree thereof was smaller than that in the corresponding group used in Example.

Regarding Examples 1 to 10, 2
No change in condition was observed when stored at 5 ° C for 6 months, and no problem skin irritation reaction was observed in a 48-hour back occlusion patch test conducted by 30 male panelists.

[0054]

INDUSTRIAL APPLICABILITY As described in detail above, according to the present invention, the stability and safety are good, and the effects of preventing rough skin and aging of the skin, and improving the effects of the same are synergistically improved.
An external preparation could be obtained.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁷ Identification code FI A61K 31/352 A61K 31/352 A61P 17/00 A61P 17/00 // C07D 311/36 C07D 311/36 (56) References Kaihei 3-188013 (JP, A) JP 8-127532 (JP, A) JP 7-196526 (JP, A) JP 9-124497 (JP, A) JP 2000-119189 (JP , A) JP 2000-119125 (JP, A) International Publication 99/047119 (WO, A1) (58) Fields investigated (Int.Cl. ⁷ , DB name) A61K 35/78 A61K 7/00 A61K 31 / 352

Claims (3)

(57) [Claims] 1. One or more kinds selected from an extract and an extracted fraction of Kacon ( Puerariae Radix ) and one kind selected from an extract and an extracted fraction of Waremoko ( Sanguisorba officinalis L.), or A skin external preparation for preventing and improving aging, which comprises two or more kinds. 2. The extract fraction of Kacon ( Puerariae Radix ) is 50% to 99.% when eluted with a mixed solvent of water and ethanol after the extract is adsorbed on a synthetic adsorption resin.
4 ', 7-dihydride eluted with 5% aqueous ethanol
The external preparation for preventing and improving aging according to claim 1, which is a fraction containing roxyisoflavone . 3. A method for preventing and improving aging, which comprises 4 ', 7-dihydroxyisoflavone and one or more kinds selected from extracts and extract fractions of Chinese cabbage ( Sanguisorba officinalis L.) . External skin preparation .