JP3418583B2 - Propolis composition and method for producing the same - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a propolis composition which is used in health foods and the like and has an enhanced effect of promoting absorption into the body, and a method for producing the same.

[0002]

2. Description of the Related Art Conventionally, as a propolis composition of this type, a soft capsule of a propolis composition containing propolis powder, lecithin and safflower oil has been known. In this soft-encapsulated propolis composition, the propolis is uniformly emulsified in the safflower oil by the emulsifying action of lecithin by adding and mixing lecithin and safflower oil to the propolis powder. The propolis emulsified in the safflower oil is easily absorbed into cells via the safflower oil having an affinity for the biological membrane. On the other hand, this propolis composition was produced by mixing a predetermined amount of propolis powder, lecithin and safflower oil and then thoroughly stirring them.

[0003]

However, in the above-mentioned conventional propolis composition , the safflower oil is an n-6 series oil and fat, and its affinity for biological membranes was not so high. On the other hand, in the conventional method for producing a propolis composition, since the propolis powder, lecithin and safflower oil are merely mixed and stirred, the emulsifying action of propolis by lecithin cannot be sufficiently enhanced.

The present invention has been made by paying attention to the problems existing in the prior art as described above. An object of the invention is to provide a propolis composition capable of enhancing the effect of promoting absorption of propolis into a living body and a method for producing the same.

[0005]

In order to achieve the above-mentioned object, the propolis composition of the invention described in claim 1 contains propolis, lecithin and perilla oil.

[0006] Production method of propolis compositions of the invention recited in claim 2, after the flop Roporisu lecithin dissolved in at least one solvent selected from alcohol and acetone, at least one selected from the vacuum and heating It is characterized in that the solvent is removed, and perilla oil is further added and mixed.

[0007] Production method of propolis compositions of the invention described in claim 3, the flop Roporisu and lecithin were dissolved in at least one solvent selected from alcohol and acetone, to form a precipitate by adding an organic solvent, perilla to the precipitation
It is characterized in that oil is added and mixed.

[0008]

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the present invention will be described in detail below. The propolis composition contains propolis and lecithin, and also contains perilla oil .

[0010] Propolis is a natural substance that constitutes the nest of bees, and resinous substances collected from various plants,
It is composed of glandular secretions from bees and plays a role in repairing and strengthening the nest and protecting the nest from bacteria and viruses. This propolis contains tars, resins, and beeswax as the main ingredients, and also has a good balance of dozens of active ingredients such as essential oils, esters such as pollen, proteins, sugars, minerals, vitamins and flavonoids. include. This propolis has been used as a folk remedy since ancient times, but in recent years, its physiological effects include antibacterial action, antiviral action, antioxidative action, antiinflammatory action, analgesic action, immunoregulatory action, antiallergic action, etc. Has been revealed.

The raw propolis mass used as a raw material for producing the propolis composition is produced in Brazil, China, Japan,
Those collected from various production areas such as the United States, European countries, and Oceania countries are used. Since this natural propolis mass contains various physiologically active substances and contaminants, an extract produced by selectively extracting a target component is usually used. Examples of the extract that constitutes the propolis composition of the present embodiment include alcohol extraction propolis, water extraction propolis, and water-soluble propolis.

[0012] The alcohol-extracted propolis contains a component dissolved in a hydrophilic organic solvent (especially alcohol) such as ethanol among the components in the original propolis mass. The alcohol-extracted propolis is produced by extracting the crushed propolis raw mass with the hydrophilic organic solvent. The water-extracted propolis contains a component dissolved in water among the components in the original propolis mass. This water-extracted propolis is produced by extracting the ground propolis mass with water.

The water-soluble propolis preferably has an average molecular weight of 20000 to 200 among the above water-extracted propolis.
000, more preferably a high molecular weight fraction within the range of 25,000 to 40,000. This water-soluble propolis is an ultrafiltration method from the water-extracted propolis,
It is produced by removing low-molecular substances using a dialysis method or an ethanol precipitation method so that the average molecular weight becomes 20000 or more. In addition, the ethanol precipitation method,
This is a method of forming a precipitate by adding 30 to 80% by volume of ethanol to the water-extracted propolis.

Lecithin has a function of emulsifying propolis in the perilla oil and is contained in order to promote absorption of propolis into the living body. This lecithin is
It has emulsifying action, dispersing action and antioxidant action,
Since it is a major constituent of biological membranes, maintenance of liver function,
It has the function of promoting absorption of physiologically active substances and fat-soluble vitamins, and functions as a source of essential fatty acids.

This lecithin is a phospholipid that is abundantly contained in soybean, egg yolk and the like, and contains phosphatidylcholine (hereinafter referred to as PC) as a main component. Also,
Examples of components other than PC contained in this lecithin include phospholipids such as phosphatidylethanolamine (PE) and phosphatidylinositol (PI).

The PC content (PC content) contained in the lecithin is preferably 20 to 70% by weight.
When the PC content is less than 20% by weight, it is necessary to add a large amount in order to increase the PC content, so that the content of propolis in the propolis composition is reduced. On the contrary, lecithin having a PC content of more than 70% by weight cannot be easily obtained because it is expensive. Furthermore, it is more preferable to use lecithin having a PC content of 50 to 70% by weight, since it is easy to use in both water and oil systems and the absorption is good.

The amount of lecithin added is preferably 0.01 to 100 parts by weight, more preferably 0.1 to 10 parts by weight, based on 1 part by weight of propolis. If the amount of lecithin added is less than 0.01 parts by weight, the amount of lecithin is too small compared to the amount of propolis,
Propolis cannot be sufficiently emulsified. Conversely 1
When it exceeds 100 parts by weight, the content of propolis in the propolis composition is reduced, which is not preferable.

Further, in order to further enhance the effect of promoting absorption of propolis into the living body, it is preferable to form a complex of propolis and lecithin. This complex is presumed to have a structure in which the surface of the alcohol-extracted propolis is coated with lecithin by intermolecular bonding of lecithin to the surface of the alcohol-extracted propolis, and both of them behave integrally. Is able to.

This complex is obtained by dissolving the alcohol-extracted propolis and lecithin in at least one solvent selected from alcohol and acetone, and then removing the solvent by at least one selected from reduced pressure and heating. . Alternatively, it can be obtained by dissolving alcohol-extracted propolis and lecithin in at least one solvent selected from alcohol and acetone, and then adding an organic solvent to form a precipitate.

[0020]

[0021]

[0022]

The amount of perilla oil added is preferably 0.01 to 100 parts by weight, more preferably 0.1 to 10 parts by weight, based on 1 part by weight of propolis. If the added amount of perilla oil is less than 0.01 part by weight, the amount of perilla oil is too small compared to the amount of propolis, so that the effect of promoting absorption of propolis into the living body cannot be sufficiently exerted. On the other hand, if it exceeds 100 parts by weight, the content of propolis in the propolis composition will decrease, which is not preferable.

The above operation will be described below. The propolis composition configured as described above is manufactured according to the first manufacturing method or the second manufacturing method described below. That is, the first production method comprises at least one of propolis and lecithin selected from alcohol and acetone.
This is a method in which after dissolving in a solvent of a seed, the solvent is removed by at least one selected from reduced pressure and heating to form a complex, and this complex is further mixed with perilla oil .

In the second production method, propolis and lecithin are dissolved in at least one solvent selected from alcohol and acetone, and then an organic solvent is added to cause precipitation (complex).
Is formed, and the precipitate is mixed with perilla oil .

In producing the propolis composition according to the first production method, first, propolis and lecithin are added to the solvent, and then they are sufficiently mixed and stirred to prepare a solution in which they are dissolved. In this solution, alcohol-extracted propolis and lecithin are almost uniformly dissolved, or some of them are intermolecularly bonded.

Next, the solution is concentrated under reduced pressure, concentrated under heating, or concentrated under reduced pressure while heating to remove the solvent, thereby forming a complex in which the surface of the alcohol-extracted propolis is coated with lecithin by intermolecular bonds. The heating temperature for removing the solvent is preferably 4
The temperature is 0 to 80 ° C, more preferably 40 to 60 ° C. If the heating temperature is lower than 40 ° C, it takes too long to remove the solvent. On the other hand, if the temperature exceeds 80 ° C., the solution may boil suddenly. In addition, the physiological activity of the active ingredient in the alcohol-extracted propolis may be lost.

Finally, by adding perilla oil to the above complex and thoroughly mixing and stirring, a paste-like or semi-solid propolis composition is obtained. This propolis composition is in a state in which each complex is uniformly emulsified in perilla oil by the emulsifying action of lecithin coating the surface of the complex.

On the other hand, when producing the propolis composition according to the second production method, first, as in the case of the first production method, a solution prepared by dissolving alcohol-extracted propolis and lecithin in the solvent is prepared. To make. Next, a precipitate is formed by adding an organic solvent consisting of n-hexane or ligroin to this solution. This precipitate is
It is presumed that the surface of the alcohol-extracted propolis is covered with lecithin as in the case of the complex in the first production method.

Subsequently, the obtained precipitate (complex) is washed with the above organic solvent preferably 5 times or more, and then dried by a known method. Finally, by adding perilla oil to the precipitate and thoroughly mixing and stirring, a propolis composition similar to that of the first production method can be obtained.

The propolis composition produced as described above is, for example, orally administered after being soft-encapsulated. At this time, the soft capsule is rapidly destroyed in the stomach and releases the content of the propolis composition. Then, the released propolis composition is absorbed by cells constituting the inner surface of the stomach and intestines.

At this time, the perilla in the propolis composition is
The oil easily contacts the cell membrane due to its high affinity and membrane permeability for the cell membrane constituting the surface of the cell, and also enhances the membrane permeability of the cell membrane to facilitate entry of the complex into the cell. Furthermore, the emulsifying action of lecithin in the propolis composition facilitates emulsification of the complex within the cell membrane. It is presumed that the alcohol-extracted propolis, which has entered a state where it easily enters the cell due to the combined action of the perilla oil and lecithin, easily enters the cell through the cell membrane and is absorbed therein.

The effects exhibited by the above embodiment will be described below. -The propolis composition of the embodiment contains propolis and lecithin, and also contains perilla oil . For this reason, since the affinity and membrane permeability of the above-mentioned perilla oil with respect to the biological membrane are very high, the effect of promoting absorption of propolis into the living body can be enhanced. Furthermore, since the propolis can be uniformly emulsified in perilla oil by the emulsifying action of lecithin, the effect of promoting absorption of propolis into the living body can be further enhanced.

In general, the absorption rate of propolis into the living body is very low, and it has been reported that the absorption rate in human blood level is about 4%. The components are excreted as urine etc. without being absorbed in the living body. Since the content of the active ingredient in this propolis is extremely small, it is inevitably necessary to ingest a large amount, which significantly increases the financial burden on the user.

On the other hand, when taking propolis as a powder, there was a problem that the content of propolis was lowered due to the addition of excipients. In addition, when taken as a tablet, the dissolution rate becomes an important issue, and there is a possibility that it may cause solidification over time due to the limited transit time of the small intestine. On the other hand, the soft capsules currently on the market are said to release the contents rapidly in the stomach, and the active ingredient is efficiently absorbed into the body. When a performance screening test was carried out, it was found that there was almost no difference in absorption rate between the tablet and the conventional soft capsule.

On the other hand, the propolis composition of this embodiment has a remarkably enhanced absorption promoting effect, so that it can exhibit a high health promoting effect and the like even when administered in a small amount as compared with conventional propolis products. . Therefore, it can be said that the propolis composition of the present embodiment is a very excellent one that can significantly reduce the financial burden on the user while ingesting a large amount of the active ingredient.

[0037]

Further, experimental results were reported comparing rats fed with a diet containing perilla oil rich in α-linolenic acid and rats fed with a diet containing safflower oil instead of perilla oil. There is. According to this report, DH in brain phospholipids was observed in rats treated with safflower oil.
As the amount of A decreased, n-6 series arachidonic acid and the like increased. On the other hand, in rats treated with perilla oil,
It was shown that the amount of DHA in the brain phospholipid was large, the correct answer rate was high in the light-dark discrimination test, and the learning ability was excellent. Therefore, the propolis composition of the present embodiment can exhibit the effect of improving learning ability in addition to the effect of promoting health by the active ingredient in propolis.

On the other hand, the linoleic acid (n-6) series fats and oils to which safflower oil, which has been most often used conventionally as an excipient for soft capsules, is converted to arachidonic acid in vivo and then the arachidonic acid is converted from the arachidonic acid. It has been clarified that many physiologically active substances (lipidic inflammation mediators) are produced and cause diseases such as allergies. On the other hand, α-linolenic acid (n-, which is used in the present embodiment,
3) series of fats and oils have been reported to reduce allergies and suppress cancer.

[0040]

On the other hand, it has been reported that the mice were fed a diet containing perilla oil, corn oil or fish oil, and tested for the period of tumor onset after administration of a carcinogen. According to this report, with corn oil, 30 days after administration,
Tumors first appeared on day 48 with perilla oil and fish oil. Further, the size (diameter) of the tumor after 8 weeks was corn oil (16 mm)> shiso oil (3.5 mm)> fish oil (2 mm).
, And the tumor suppressive effect has been confirmed for n-3 series oils and fats. Therefore, the use of n-3 series oils and fats can also exert a tumor suppressive effect.

The first method for producing a propolis composition is to dissolve propolis and lecithin in at least one solvent selected from alcohol and acetone, and then remove the solvent by at least one selected from reduced pressure and heating, Furthermore, perilla oil is added and mixed. Second
In the method for producing a propolis composition, the propolis and lecithin are at least one selected from alcohol and acetone.
After dissolving in a seed solvent, an organic solvent is added to form a precipitate, and the oil is added to the precipitate and mixed.
Therefore, a propolis composition capable of enhancing the effect of promoting absorption of propolis into the living body can be easily produced. In particular, in the process of manufacturing the propolis composition,
By forming a complex of propolis and lecithin, the effect of promoting absorption of propolis into the living body can be further enhanced.

[0043]

EXAMPLES Examples and comparative examples embodying the above embodiment will be described below. (Comparative Example 1) 10 kg of original Brazilian propolis mass was crushed by a crusher, and an ethanol solution containing 20 liters of 95% by volume of ethanol (hereinafter referred to as ethanol only) was stirred for 3 hours. Next, the ethanol solution was centrifuged with a centrifuge and then filtered to collect a supernatant. Then, the propolis concentration in the supernatant was adjusted to 20% by weight to obtain 20% by weight.
A weight percent alcohol extracted propolis was obtained.

Comparative Example 2 To 1250 ml of alcohol-extracted propolis of Comparative Example 1, 50 g of lecithin having a PC content of 70% by weight (hereinafter referred to as lecithin only) and 500 ml of ethanol were added and stirred, and then the solvent amount was about 5
The solution was concentrated under reduced pressure until the concentration became 0.1%, then a sufficient amount of n-hexane was added and left overnight to form a precipitate. Next, the precipitate was recovered, washed with n-hexane five times or more, and then naturally dried, whereby 300 g of a complex having a weight ratio of propolis and lecithin of about 1: 0.2 was obtained.

Example 1 By adding 60 g of the composite of Comparative Example 2 to 250 g of perilla oil and thoroughly mixing and stirring, 310 g of a propolis composition having a weight ratio of propolis to perilla oil of about 1: 5 was obtained. Was given. The concentrations of propolis, lecithin and perilla oil in this propolis composition are 16%, 3% and 81% by weight, respectively.

Example 2 To 150 ml of alcohol-extracted propolis of Comparative Example 1, 10 g of lecithin and 100 ml of ethanol were added and stirred, and the mixture was concentrated under reduced pressure until the solvent amount became about 5%, and then a sufficient amount of n-hexane was added. Was added and allowed to stand overnight to form a precipitate. Next, the precipitate was recovered, washed with n-hexane five times or more, and then naturally dried to obtain 40 g of a complex having a weight ratio of propolis and lecithin of about 1: 0.3. To this complex, 150 g of perilla oil was added, and the mixture was thoroughly mixed and stirred to give a propolis composition 190 having a weight ratio of propolis to perilla oil of about 1: 5.
g was obtained. The lecithin concentration in this propolis composition is about 5.3% by weight. The concentrations of propolis, lecithin and perilla oil in this propolis composition are 16%, 5% and 79% by weight, respectively.

Example 3 To 250 ml of alcohol-extracted propolis of Comparative Example 1, 10 g of lecithin and 100 ml of acetone were added, and the mixture was stirred, concentrated under reduced pressure until the amount of the solvent became about 5%, and then a sufficient amount of ligroin was added. And left overnight to form a precipitate. Next, the precipitate was recovered, washed with ligroin five times or more, and then naturally dried to obtain a complex 60 having a weight ratio of propolis and lecithin of about 1: 0.2.
g was obtained. By adding 250 g of perilla oil to this complex and thoroughly mixing and stirring, 310 g of a propolis composition having a weight ratio of propolis to perilla oil of about 1: 5 was obtained. The concentrations of propolis, lecithin and perilla oil in this propolis composition are 16% by weight, 3% by weight and 81% by weight, respectively.

Example 4 To 250 ml of alcohol-extracted propolis of Comparative Example 1, 10 g of lecithin and 100 ml of acetone were added, stirred, and then concentrated under reduced pressure while heating to 50 ° C. until a paste was formed. 60 g of a pasty complex with a weight ratio of lecithin of approximately 1: 0.2 was obtained. By adding 250 g of perilla oil to this complex and thoroughly mixing and stirring, 310 g of a propolis composition having a weight ratio of propolis to perilla oil of about 1: 5 was obtained. The concentrations of propolis, lecithin and perilla oil in this propolis composition are 16% by weight, 3% by weight and 81% by weight, respectively.

Comparative Example 3 By adding 50 g of safflower oil to 12 g of the composite of Comparative Example 2 and mixing and stirring, 62 g of a mixture of propolis and safflower oil in a weight ratio of about 1: 5 was obtained. .
The concentrations of propolis, lecithin and safflower oil in this mixture are 16%, 3% and 81% by weight, respectively.

[0050] The complex 12g (Comparative Example 5) Comparative Example 2, by mixing stirred with coconut oil 50 g, weight ratio of propolis and coconut oil approximately 1: Mixture 62g are obtained of 5 Was given. The concentrations of propolis, lecithin and coconut oil in this mixture are 16%, 3% and 81% by weight, respectively.

[0051] The complex 12g (Comparative Example 6) Comparative Example 2, by mixing stirred with sardine oil 50 g, weight ratio of propolis and sardine oil approximately 1: mixture of 5 62 g was obtained . This mixture
The concentrations of propolis, lecithin and sardine oil in the mixture are
They are 16% by weight, 3% by weight and 81% by weight, respectively.

Example 7 To 12 g of the composite of Comparative Example 2 was added 50 g of perilla oil and the mixture was stirred to obtain 62 g of a propolis composition having a weight ratio of propolis to perilla oil of about 1: 5. It was
Propolis The propolis composition, lecithin and teeth
The soybean oil concentrations were 16% by weight, 3% by weight and 81% by weight, respectively.
% By weight.

<Comparison Experiment of Propolis Absorption Rate Using Intestinal Inverted Pouch> (Comparison of Propolis Absorption Effect by Lecithin Concentration) Alcohol-extracted propolis of Comparative Example 1 and the propolis compositions of Examples 2 and 3 were combined with Ringer solution (140 mM). NaCl, 10 mM K
HCO ₃ , 0.4mM KH ₂ PO ₄ , 2.4mM K ₂ HPO ₄ , 1.2mM CaCl ₂・ 2H ₂
O, 1.2 mM MgCl ₂ .6H ₂ O, 1 mM glucose (pH 7.4)) was added to prepare samples each having a propolis concentration of 0.4% by weight. In order to examine the absorption-promoting effect of each of these samples, the absorption rate (%) of propolis was measured using a rat intestinal everted sac as described below.

Wistar male rats (body weight about 200
g) was anesthetized with ether and the cervical cord was cut with scissors. A laparotomy was performed at the midline, the terminal part of the ileum was cut, and the mesentery adhering to it was excised while picking up the end of the ileum, and the entire small intestine was extracted. The excised small intestine was divided into 6 equal parts, each of which was passed through a stainless rod to wash the surface cleanly to remove water, and then inverted. After washing with physiological saline about twice, Ringer's solution was injected from the open end of the rod, and the open end was ligated with a cotton thread.

Next, 5 ml of each sample was injected.
A reversal sac was inserted into a 0 ml Erlenmeyer flask, a mixed gas of 95% O ₂ and 5% CO ₂ was bubbled for 10 minutes, and then the mixture was incubated at 37 ° C. for 30 minutes. At that time, the Erlenmeyer flask was appropriately vibrated back and forth. After 30 minutes, the inversion bladder in the flask was taken out, the water on the surface was removed, and then the liquid in the inversion bladder was collected and analyzed by high performance liquid chromatography (HPLC),
The area of the entire peak corresponding to propolis was measured, and this area was used as the propolis absorption concentration in the inversion sac.

On the other hand, the area of the entire peak corresponding to propolis in each sample was measured by performing HPLC analysis on each sample in the same manner, and this area was defined as the initial concentration of propolis in the sample. Then, the propolis absorption rate (%) for each sample is calculated by the following formula (1) using the propolis absorption concentration and the propolis initial concentration.
The results are shown in Table 1 as well as calculated according to

[0057]

[Equation 1]

[0058]

[Table 1] In addition, the absorptance in Table 1 and the following table shows the average value of the results of 5 times (n = 5) tests performed on each sample and its standard deviation (Mean ± SD). In addition, the statistical processing uses a Student t-test,
In the table, * indicates that the significant difference from Comparative Example 1 is less than 5% (p <0.05), and ** indicates the significant rate that Comparative Example 1 is less than 1% (p <0.01). Is shown.

From the results shown in Table 1, the lecithin concentration was 3% by weight.
It was shown that Example 1, which had the highest absorption rate of propolis, was the most effective. In addition, Example 1 is 3 times as compared with Comparative Example 1.
It was also shown that there was a more than double absorption-promoting effect.

[0060] (up Roporisu comparison absorbing effect) alcohol extract propolis Comparative Example 1, Comparative Example 3, 5,
Mixture of 6, and implementation in propolis composition of Example 7, Ringer's solution were added each propolis concentration of 0.4 wt%
A sample prepared so that In order to examine the absorption-promoting effect of each of these samples, the absorption rate (%) of propolis was measured by HPLC analysis using a rat intestinal inversion sac as in the above-mentioned (comparison of propolis absorption effect by lecithin concentration) test. . The results are shown in Table 2.

[0061]

[Table 2] From the results in Table 2, it was shown that those containing coconut oil, sardine oil or perilla oil had a very high absorption promoting effect as compared with Comparative Example 3 containing safflower oil. further,
It was shown that the propolis composition containing perilla oil of Example 7 had a prominently high propolis absorption effect.

( Comparison of Propolis Absorption Effect in Blood ) To the alcohol-extracted propolis of Comparative Example 1, the mixture of Comparative Example 3, and the propolis composition of Example 7, Ringer's solution was added to obtain propolis concentrations of 3% by weight, respectively. A sample prepared so that The in vivo absorption promoting effect of each of these samples was examined as follows.

Fasted male Wistar rats (body weight: about 200 g) were divided into 3 groups, and 60 mg (300 mg / kg) of each sample was orally administered to each group of rats. 0.5, 1, 2, 4 and 6 immediately before administration and after administration
After a lapse of time, serum was separated from blood collected from the abdominal vena cava, the serum was analyzed by HPLC, and the area of the entire peak corresponding to propolis in each serum was measured. And

On the other hand, a standard propolis solution was prepared by preparing the alcohol-extracted propolis of Comparative Example 1 at a concentration of 1.0 mg / ml, and this solution was subjected to HPLC analysis in the same manner as above to determine the total peak corresponding to propolis. Was measured, and this area was used as a standard propolis concentration.
Then, using the standard propolis concentration, the converted concentration (μg / ml) obtained by converting the serum propolis concentration into the concentration was calculated according to the following calculation formula (2), and a graph showing the relationship between the elapsed time and the converted concentration is shown. Shown in 1.

[0065]

[Equation 2] Furthermore, for each sample on the graph shown in FIG.
The area AUC _0-6h (μg · min / ml) under the serum concentration curve for 0 to 6 hours was calculated. The results are shown in Table 3.

[0066]

[Table 3] As shown in FIG. 1, it was shown that the mixture containing the alcohol-extracted propolis of Comparative Example 1 and the safflower oil of Comparative Example 3 reached the maximum blood concentration 1 hour after administration, and then gradually disappeared. It was On the other hand, the propolis composition of Example 7 showed a higher blood concentration immediately after administration than Comparative Examples 1 and 3 and had a very high blood concentration even 6 hours after the administration. Was shown to have been sustained. Further, as shown in Table 3, in comparison of AUC _0-6h , it was shown that Example 7 has a high absorption effect of 10 times or more as compared with Comparative Example 1.

Further, the technical idea which can be understood from the above embodiment will be described below.

( 1 ) The propolis composition according to claim 1, which is orally administered in a form of soft capsule. In this case, since the propolis composition is in a paste form or a semi-solid form, it can be easily soft-encapsulated. Further, since the propolis composition is rapidly released in the stomach, the immediate effect is high, and since the transit time to the small intestine can be lengthened, the effect of promoting absorption into the body can be further enhanced.

(2) The method for producing a propolis composition according to claim 2 or 3 , wherein the organic solvent is n-hexane or ligroin. With this structure, a complex of propolis and lecithin can be easily formed.

(3) The method for producing a propolis composition according to any one of claims 2 , 3 and (2), wherein the propolis is alcohol-extracted propolis. When constituted in this way, a propolis composition capable of enhancing the effect of promoting absorption of propolis into the living body can be more easily manufactured.

[0071]

[0072]

As described in detail above, the present invention has the following effects. According to the propolis composition of the invention described in claim 1, the effect of promoting absorption of propolis into the living body can be enhanced.

[0073]

According to the method for producing a propolis composition of the invention described in claims 2 and 3, it is possible to easily produce a propolis composition capable of enhancing the effect of promoting absorption of propolis into a living body. I can .

[Brief description of drawings]

FIG. 1 is a graph showing changes in serum propolis absorption concentration in Examples.

─────────────────────────────────────────────────── ─── Continuation of front page (72) Inventor Satoshi Mishima 1-1, Kano Sakurada-cho, Gifu-shi Api Co., Ltd. (56) References JP-A-9-149765 (JP, A) JP-A-5-59391 ( JP, A) Keiko Hashimoto, Junko Shimada, Food ingredients and seasonings, Japan, Asakura Shoten Co., Ltd., August 1993, 1-4 (58) Fields investigated (Int.Cl. ⁷ , DB name) A23L 1/00-3/54 Food related literature information (food net)

Claims (3)

(57) [Claims] 1. A propolis composition containing propolis, lecithin and perilla oil . 2. After dissolving propolis and lecithin in at least one solvent selected from alcohol and acetone, the solvent is removed by at least one selected from reduced pressure and heating, and perilla oil is further added and mixed. A method for producing a propolis composition characterized by the above. 3. After the propolis and lecithin were dissolved in at least one solvent selected from alcohol and acetone, to form a precipitate by adding an organic solvent, perilla its precipitation
A method for producing a propolis composition, which comprises adding and mixing oil .