JP3351035B2 - Denture cleaner - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a denture cleaner containing an active oxygen generating substance and dextranase. More specifically, the present invention relates to a dextranase produced by active oxygen by using a specific dextranase. The present invention relates to a denture cleaning agent that prevents a decrease in the activity of a denture and exhibits excellent denture plaque removing power.

[0002]

2. Description of the Related Art In recent years,
With the advent of an aging society, the use of dentures tends to increase year by year. When using a denture, food debris is easily accumulated between the denture and the oral mucosa, and the inside of the oral cavity is more likely to be dirty than a healthy person. In particular, denture plaques of denture users show more Candida bacteria than dental plaques of healthy persons, and these are considered to be the cause of denture stomatitis. In addition, the presence of denture plaque serves as a home for bacteria, causes unpleasant denture odor, and is a root cause of tartar generation, and forms stubborn dirt.

[0003] Conventionally, as a cleaning agent for dentures, a foaming type in which an active oxygen generating agent is blended as a bleaching agent is generally used. The effect is insufficient.

[0004] Here, the formation mechanism of denture plaque generated on dentures and dental plaque generated on natural teeth is considered to be the same, and is basically considered to be the same as plaque. In this case, the plaque can be decomposed by an enzyme, and dextranase is known as such an enzyme. In fact, in dentifrices and mouthwashes, those containing dextranase as a plaque-decomposing enzyme are conventionally known.

[0005] However, when dextranase is added to a denture cleaner containing an active oxygen generator, there is a problem that active oxygen reduces the activity of dextranase. That is, enzymes such as dextranase are composed of proteins, but active oxygen has a strong oxidizing power, and denatures proteins to lower the activity of the enzymes.

The present invention has been made in view of the above circumstances, and uses an active oxygen generating substance and dextranase in combination,
Further, it is an object of the present invention to provide a denture cleaning agent that exhibits excellent denture plaque removing power by preventing a decrease in dextranase activity due to active oxygen.

[0007]

The present inventors have conducted intensive studies to achieve the above object, and as a result, it has been found that dextranase derived from the genus chaetomium sp has good resistance to active oxygen. It has been found that denture plaque removal effect of dentures can be effectively exhibited without reducing activity even in the presence of active oxygen.

That is, as shown in Examples and Comparative Examples described later, several kinds of dextranases of different origins are blended in a denture cleaning agent containing an active oxygen generating substance, and the storage stability and denture plaque removing power of the same are determined. As a result of the evaluation, dextranase derived from the genus Ketomium specifically exhibited excellent storage stability even in the presence of active oxygen, and also exhibited excellent denture plaque removing power. In particular, condensed phosphate (here, tripolyphosphate) It has been found out that an excellent effect of removing denture plaque can be obtained when used in combination with sodium (using sodium), and the present invention has been completed.

Accordingly, the present invention provides an active oxygen generating substance,
The present invention provides a denture cleanser characterized by comprising a dextranase derived from the genus Ketomium. Further, as a preferred embodiment, a denture cleaning agent further containing a condensed phosphate is provided.

[0010] It is already known to mix dextranase as a plaque decomposition / removal component with a denture cleaner containing an active oxygen-generating substance, but a conventional dextranase-containing denture cleaner contains active oxygen. In order to prevent deactivation of dextranase, tablets are divided into an enzyme layer (dextranase, etc.) and an oxygen layer, and the enzyme layer is dissolved first to reduce the chance of contact between the enzyme and active oxygen. Efficient use of enzymes (Japanese Patent Application Laid-Open No. 51-38415), powdered enzymes in which the enzyme is mixed with a specific protective agent,
Protective agents that act as a dissolution retarder for the enzyme so that the enzyme works effectively after the action of active oxygen is reduced or eliminated (Japanese Patent Application Laid-Open No. 57-142910). The specific dextranase has good resistance to active oxygen, and therefore, even when used in combination with an active oxygen generating substance, can maintain the activity of dextranase without needing to take special measures. Exhibiting the effect of removing denture plaque, and further improving the effect of removing denture plaque by using together with the condensed phosphate, is related to the present inventors' new findings, and the present invention is based on such new findings. It was made based on.

Hereinafter, the present invention will be described in more detail.
As described above, the denture cleaner of the present invention is obtained by mixing a dextranase derived from the genus Ketomium with a denture cleaner containing an active oxygen-generating substance, and is preferably obtained by further mixing a condensed phosphate. is there.

As the above-mentioned active oxygen generating substance, the same substances as those used as bleaching agents in ordinary denture cleaners can be used, and specifically, potassium monohydrosulfate (oxone), perboric acid Examples thereof include sodium (perbon) and sodium percarbonate, and these can be used alone or as a mixture of two or more. The amount of these active oxygen generating substances is not particularly limited, but is usually 10 to 80% by weight, preferably 20 to 70% by weight. If the amount of the active oxygen generating substance is less than 10% by weight, a satisfactory bleaching effect may not be obtained.
If the content is more than 100% by weight, the total balance as a denture cleaning agent may cause a decrease in quality, such as a decrease in bubble power.

Next, the dextranase to be added to the denture cleaning composition of the present invention as a component for decomposing and removing denture plaque is derived from the genus chaetomium sp. Bacteria include Ketomium g
racile, ketomium spirale (Chaetomium spira)
le), Ketomium indicu
m), ketomium olivaceum (Chaetomium olivaceu)
m) and the like, and among these, dextranase obtained from ketomium gracile is preferably used because it has a particularly excellent denture plaque removing power.

The amount of the dextranase derived from the genus Ketomium is not particularly limited, but is preferably 0.1 to 30% by weight, particularly 0.5 to 20% by weight of the whole detergent. When the amount is less than 0.1% by weight,
In some cases, a sufficient effect of removing denture plaque cannot be obtained. On the other hand, even if it exceeds 30% by weight, no significant improvement in the effect can be seen, which is disadvantageous in terms of economy.

Further, the denture cleaning agent of the present invention can further improve the effect of removing denture plaque by blending a condensed phosphate. Examples of the condensed phosphate include sodium pyrophosphate, sodium tripolyphosphate, sodium acid polyphosphate, and the like, and one or more of these may be blended. The amount of these condensed phosphates is not particularly limited, but is preferably 1 to 30% by weight, particularly 3 to 20% by weight, and if less than 1% by weight, the combined use with the above-mentioned dextranase is required. In some cases, the effect cannot be obtained. On the other hand, if the amount exceeds 30% by weight, the combined effect reaches saturation, and no improvement is observed.

The denture cleanser according to the present invention contains the active oxygen-generating substance, dextranase derived from the genus Ketomium and optionally a condensed phosphate, and is usually prepared in the form of powder, granules, tablets and the like. It is used by dissolving it in water. In this case, the denture cleanser of the present invention may contain known components together with the above components.

For example, by blending a carbonate such as sodium hydrogen carbonate, sodium carbonate, potassium carbonate and the like with an acid agent such as citric anhydride, succinic acid and malic acid, the mixture is foamed at the time of dissolution, and the detergent is dissolved and dispersed. Can be promoted. In addition, a surfactant can be added to improve the detergency and ensure appropriate foaming, and specific examples of the surfactant include sodium linear alkylbenzene sulfonate and sodium lauryl sulfate.
Furthermore, polyvinylpyrrolidone, lactose, and the like can be added as a pH adjuster, a pigment, and a shaping agent for forming a tablet. The denture cleaning agent of the present invention is preferably adjusted so that the pH of the solution at the time of use is in the range of 5 to 11, thereby exhibiting an excellent denture plaque removing effect.

[0018]

EXAMPLES The present invention will be described in detail below with reference to examples and comparative examples, but the present invention is not limited to the following examples. [Examples 1, 2 and Comparative Examples 1 to 5] Denture cleansing compositions having the compositions shown in Table 2 were produced using seven types of dextranase obtained from the bacteria shown in Table 1. The storage stability and denture plaque removal power of dextranase in the obtained denture cleanser were evaluated by the following methods. Table 1 shows the results.

[0019]

[Table 1]

[0020]

[Table 2]

[0021]Storage stability evaluation method Apply 50 g of denture cleanser to 100 mL of polypropylene
After putting in a closed container and making a hole of about 1 mm in the mouth,
Store at 0 ° C, 75% humidity, and store for 1 month.
Strannase activity was determined by the Somogyi-Nels method.
on method), and based on the residual ratio,
The qualitative evaluation was made. Denture plaque removal power evaluation method Candida albicans in test tube
And Streptococcus sobrinas
inus) (turbidity OD = 0.1 at 550 nm)
Concentration) and 1% sucrose solution
BHI medium (37 g BHI + 10 g sucrose /
L) (concentration L).
傾斜 anaerobic culture at 37 ° C for 20 hours
Plaques were prepared. Then, wash twice with distilled water and try
Remove any artificial plaque that is incompletely adhered to the test tube wall,
After adding 4 ml of distilled water to the test tube, wash the denture
100 mg of the agent was added, and the mixture was allowed to act at 37 ° C. for 6 hours. 6 o'clock
After the elapse of time, the inside of the test tube is washed twice with distilled water, and then remaining
Plaques are dispersed with ultrasound and turbidity (OD = 550)
nm) and denture cleaner without enzyme
Separately and measure turbidity (OD control) in the same way
And calculate the denture plaque removal rate according to the following formula:
Issued. Then, according to the following criteria,
The removal power was evaluated. Removal rate (%) = {(OD control−OD sample) / O
D control} × 100  

From the results shown in Table 1, the dextranase derived from the genus Ketomium shows excellent storage stability even in the presence of active oxygen, and the denture cleaner of the present invention containing the dextranase derived from the genus Ketomium is It was confirmed that the composition had an excellent denture plaque removing effect despite being used in combination with an active oxygen generating substance.

[Examples 3, 4 and Comparative Example 6] Denture cleaning agents having the same composition as in Examples 1 and 2 and Comparative Example 3 were prepared except that sodium tripolyphosphate was not blended. The denture plaque removing power of these denture cleaners was evaluated in the same manner as described above. Table 3 shows the results.

[0024]

[Table 3]

By comparing the results in Table 3 with the results in Table 1 above, the combined use of dextranase derived from the genus Ketomium and sodium tripolyphosphate (condensed phosphate) improves the denture-purark removing effect. It was confirmed that.

[Examples 5 to 8] Table 4 shows other prescription examples of the denture cleaner of the present invention. In addition, the compounding quantity of each component in Table 4 is weight%.

[0027]

[Table 4]

[0028]

EFFECTS OF THE INVENTION The denture cleanser of the present invention has excellent denture plaque removing power by using an active oxygen generating substance and dextranase in combination and preventing a decrease in dextranase activity due to active oxygen. It is.

Continued on the front page (58) Fields surveyed (Int. Cl. ⁷ , DB name) A61K ^7/ 00-7/50 BIOSIS (DIALOG) CA (STN) JICST file (JOIS) WPI (DIALOG)

Claims (2)

(57) [Claims] 1. A denture cleaner comprising an active oxygen generating substance and dextranase derived from the genus Ketomium. 2. The denture cleanser according to claim 1, further comprising a condensed phosphate.