JP3306750B2 - Lipolysis accelerator - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a lipolysis promoter useful for suppressing or preventing fat, improving obesity, and reducing local or systemic adipose tissue. More specifically,
The present invention relates to a lipolysis promoter containing an extract obtained from a specific plant as an active ingredient.

[0002]

2. Description of the Related Art Excessive fat accumulation is caused by accumulation of excess intake energy relative to consumed energy as white fat cells. It is a cause of various lifestyle-related diseases and is not preferable from the viewpoint of beauty. Conventionally, oolong tea and Tochu tea or an extract from seaweed have been used in the form of cosmetics or the like for the control, prevention and improvement of obesity, but they have not been effective enough. Also, in view of the recent trend of health-oriented, attempts have been made to search for lipolysis accelerators from natural products, not synthetic products. Japanese Patent Application Laid-Open No. 8-81382 discloses a lipolysis accelerator characterized by containing a citrus plant as an active ingredient. JP-A-8-301780 discloses an agent comprising a thistle plant as an active ingredient.
No. 5410 contains the same as a pepperaceous plant as an active ingredient, and Japanese Patent Application Laid-Open No. 9-95452 further contains at least one component selected from the group consisting of Huangbai extract and Oren extract as an active ingredient. Lipolysis accelerators are disclosed. However, their effects are not enough.

[0003]

Therefore, the present invention promotes the decomposition of fat in accumulated adipocytes, suppresses, prevents and improves obesity, and is a highly safe novel lipolysis derived from nature. An accelerator is provided.

[0004]

In view of such circumstances,
The present inventors have results of extensive studies, full Kitanpopo leaves to promote the decomposition of fat cells, found that it is possible to perform the suppression of obesity, the prevention and improvement, and completed the present invention.

Namely, the present invention is Ru der provides a lipolysis promoter comprising full Kitanpopo leaves as an active ingredient.

[0006] Coltsfoot Pops Tussilage vul
garis is the Asteraceae plant, that has been used from flowers or flower and leaf water, and extracted with such Bro propylene glycol, such as in cosmetics raw materials.

[0007] Such plants are used as raw juice, dried powder or solvent extract. For extraction of components from plants, water or methanol, ethanol,
An organic solvent such as isopropyl alcohol, butanol, ethyl acetate, and acetone, or a mixed solvent thereof may be used. Moreover, you may degrease with diethyl ether etc. as needed. The obtained extract components are appropriately concentrated, purified,
It can be used after sterilization and drying.

[0008]

Industrial Applicability The present invention is a novel lipolysis promoter which is highly safe and excellent in nature and has a clear lipolysis accelerating action in adipose tissue, and is excellent in suppressing, preventing and improving obesity. Has an effect.

[0009]

EXAMPLES The present invention will be described below in detail with reference to examples, but the present invention is not limited to these examples. (Example 1) 70% 10g a full Kitanpopo leaves prepared pulverized plant extract methanol 5
Repeat the ultrasonic extraction three times with 0 ml for 20 minutes,
After the extract was concentrated under reduced pressure, suspended in water, after diethyl ether defatted, freeze dried and concentrated under reduced pressure to give the full Kitanpopo leaf extract 1.8 g.

Example 2 Using the extract described in Example 1, its lipolysis-promoting effect was examined by the following test method. Table 1 shows the results. Test Method The method of Rodbell et al. (M. Rodbell et.
l. , J. et al. Biol. Chem. 239 , 375-38
0 (1964)), free adipocytes were prepared from male epididymal adipose tissue of SD rats using a collagenase solution. A mixed solution 95 of 2 ml of this free fat cell and 23 ml of a Klepslinger buffer solution of 4% bovine serum albumin.
0 μl and the extract concentration is 1000 μ
50 μl of a sample aqueous solution prepared by adding water to give g / ml was added, and the mixture was incubated at 37 ° C. for 30 minutes. 200 μl of 10% trichloroacetic acid and 100 μl of chloroform were added to the reaction solution to stop the reaction. 15,000
After centrifugation at 10 rpm for 10 minutes, the released glycerol in the supernatant was removed by the method of Bobies and Maume (LH Boobi).
s and R.S. J. Maughan, Clinica
Chimica Acta, 132 , 173-179
(1983)). As a control, water was used instead of the sample aqueous solution. Table 1 shows the amount of glycerol released from rat epididymal fat cells.

[0011]

[Table 1]

[0012] full Kitanpopo leaf extract, was released glycerol triglycerides contained in the fat cells. This
From the results, it was clarified that there was a lipolysis promoting action .

Continuation of front page (56) References JP-A-8-81382 (JP, A) Ramesh C. Arora et al, Acurus Calamus a Lipid lowering, Zywiene Czlowieka Metabolizm, 1987, Vol. 14, No. 2, pp. 81-83 (58) Field surveyed (Int. Cl. ⁷ , DB name) A61K 35/78 BIOSIS (DIALOG) CA (STN) MEDLINE (STN)

Claims (2)

(57) [Claims] 1. A lipolysis promoter comprising a full Kitanpopo leaves as an active ingredient. 2. A lipolytic enhancer according to claim 1, wherein comprising a full Kitanpopo leaf extract as an active ingredient.