JP3257091B2 - Anti-skin cancer agent or food - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[Object of the invention]

The present invention relates to an anti-skin cancer agent and an anti-skin cancer functional food containing a soy whey fraction as an active ingredient.

[0002]

[Prior art]

(1) Background of the Invention Soy whey component accounts for 7 to 9% of the total protein in soybean grain.
And various bioactive substances such as trypsin inhibitor, hemagglutinin, goitrogen, saponin, lipoxygenase, β-amylase, protease, and phytase. Among these components, saponin contains a triterpenoid-based aglycone and may be used as a raw material of a physiologically active substance, but has not been effectively used so far.

Further, trypsin inhibitors inhibit the activity of trypsin, and hemagglutinin is considered to be an unfavorable “antitrophic factor”, such as damaging the intestinal epithelial cell mucosa. These facts are also passive reasons that hinder the validity of soy whey fractions.

However, it has been reported that the soybean grain component has an anticancer effect. For example, Troll et al. (Carcino
genesis, 1, 469-472 (1980) observed that the development of breast cancer was suppressed in rats fed whole soy protein.
Becker (Carcinogenesis.2.1213-1214 (1981)
He reports that 100% of the liver cancer in mice observed on a% casein diet was completely suppressed when about 5% of the casein was replaced with whole soy protein. From these research results, it is supposed that there is some factor in soybean protein component that inhibits cancer growth, and Troll (JNCI 7, 1245-125)
0 (1984) epidemiologically considers that the anticancer substance may be a protease inhibitor contaminating soy protein.

[0005] However, while the anticancer effects of protease inhibitors are evaluated as described above, there are conflicting reports that pancreatic cancer is likely to occur in rats bred for a long time on a diet containing trypsin inhibitor, which belongs to the category of protease inhibitors. (For example, MaGuinenes
et.al., Scand. J. Gastroenterol. 15, 497-502 (198
0) etc.), it is speculated that binding of the protease inhibitor to the corresponding protease will have a negative nutritional effect. However, there is no study on the pharmacological action of soy whey components. In addition, whey is a by-product in the extraction of soybean protein in a large amount as a by-product.
It also has important significance in the agricultural processing industry.

[0006]

SUMMARY OF THE INVENTION In view of the above circumstances, an object of the present invention is to effectively utilize a soybean whey fraction as a raw material for medicines or health foods.

[0007]

Means for Solving the Problems Concept of the Invention The present inventor has studied the medicinal and nutritional side effects of the same fraction from the viewpoint of effective use of soybean whey. Not only is effective against but also does not show any particular nutritional adverse effects,
The present invention has been completed.

[0008] That is, the present invention provides an anti-skin cancer agent containing a soy whey fraction as an active ingredient and an anti-skin cancer food containing a soy whey fraction as a functional ingredient. Hereinafter, the experimental facts that led the inventors to reach the present invention will be described.

(1) Experiment Experiment 1 (Preparation of a drug) 200 kg of soybean whey was adjusted to pH 5 to 6, and concentrated by ultrafiltration at 50 ° C. DU50 made by Daicel Chemical as an ultrafiltration membrane
(Fraction molecular weight: 40,000, tubular module) and concentrated 20-fold. The obtained concentrated whey was adjusted to pH 7 with caustic soda and then spray-dried to obtain 500 g of concentrated whey powder. [Analytical value] Crude protein content about 61.4% (by the Kjeldahl method) Total sugar 21.5% (by the phenol-sulfuric acid method) Trypsin inhibitor activity 490 U / g (using the substrate BAPA, using the Katade method)

Experiment 2 (Animal test) Method 30 ICR male mice (6 weeks old) were divided into two groups, and 20% isolated soybean protein (SPI; Fujipro ^RR ) diet or 18% S
PIs were fed a diet containing 2% of the concentrated whey powder obtained in the previous example for the entire period. All diets include 5% soybean oil, 5% mineral mix (Oriental Yeast), 1% vitamin mix
(Manufactured by Oriental Yeast), cellulose 2% and DL-
Methionine was contained at 0.4%, and the balance was carbohydrate (α-corn starch: sugar = 2: 1).

One week after the experimental meal, the animal's back was shaved,
Approximately half of the mice have an acetone solution of 7,12-dimethylbenzoanthracene (100 μg / 0.
1 ml) was applied. The hair at the application site was then sheared every two weeks with scissors. In addition, three mice were bred in one cage. Body weight and food intake were measured weekly.

One week after the application of the initial factor, mice in the experimental group were given a solution of phorbol-12-myristate-13-acetate (TPA) in acetone (2.5 A).
μg / 0.1 ml) was applied twice a week for the entire duration of the experiment. The number and volume of the tumors having a diameter of 1 mmφ or more were measured every week. Tumor volume can be measured using calipers vertically (a), horizontal (b),
The height (c) was measured and calculated based on the formula of a × b × cπ / 6.

Results and Discussion FIG. 1 shows body weight and food intake in the 14th week of rearing.
No significant differences in body weight and food intake were found between the SPI group and the whey group.

FIG. 2 shows the proportion of tumor mice 14 weeks after application of the initial factor. The proportions of the SPI group and the whey group were 80% and 47%, respectively, which were significantly lower in the whey group.

FIG. 3 shows the number of tumors per tumor-bearing mouse. 4.7 each in SPI group and whey group
At 1.6, the whey group was significantly lower.

FIG. 4 shows the tumor volume of the tumor-bearing mouse.
The values for the SPI group and the whey group were 50 and 8 mm ³ , respectively.
It was significantly smaller in the whey group.

DISCUSSION The results of this experiment indicate that the addition of concentrated whey powder to diet significantly suppresses skin cancer in mice coated with a chemical carcinogen. A remarkable finding derived from this experiment is that the administration of a small amount of soy whey fraction that does not impair the food intake and growth (weight gain) of test animals shows a sufficient inhibitory effect on skin carcinogenesis. That is. Therefore, by continuously ingesting a small amount thereof, the occurrence of skin cancer or its precancerous state could be suppressed or suppressed. However, a close correlation has been observed between the occurrence of skin cancer and the amount of irradiated ultraviolet light, such as methane gas.
Since it is predicted that the ozone layer destruction due to artificially uncontrollable factors will become a serious health problem in the future, soybean whey fraction is used as an anti-skin cancer agent or anti-skin cancer function. Use as food is a good idea for two birds with one stone, which on the other hand is related to the reduction of industrial waste.

(1) Soy Whey Fraction In the present invention, the term “soy whey fraction” means, in principle, a concentrated soybean protein material such as defatted soybean, which is extracted from water or alkaline water and mainly composed of glycinin. , Which means the supernatant from which the precipitate precipitated by changing to around the isoelectric point (pH about 4.5) is removed, but from which contaminating low molecular substances (mainly salts, monosaccharides and oligosaccharides) are removed. Is preferred. Filtration with an ultrafiltration membrane is advantageous for the purpose of the invention, because it can achieve the purpose of dehydration and concentration as well as desalting and desalting relatively easily. The performance of the filtration membrane may be selected from those having a limit molecular weight of about 10,000 to 40,000.

In the above-described separation and purification of the soybean whey fraction from soybean protein, it is desirable to avoid heating as much as possible in order to prevent the decomposition of the trypsin inhibitor. However, since there is no preservability in an aqueous solution state, it should be stored in a dehydrated state by a mild drying means such as spray drying and freeze drying.

(2) Dosage Form The anti-skin cancer agent according to the present invention is administered alone as a drug or as a mixture with other drugs or excipients or as a food mixed with any food. Suitable pharmaceutical forms are tablets or granules, but may also be in the form of solutions such as solutions, elixirs and syrups. When it is a drug,
It is preferable to coexist various vitamins and minor nutrients such as minerals, especially vitamins B such as thiamine, riboflavin, nicotinamide, adeloxin, pantothenic acid, folic acid, vitamins such as vitamin C, vitamin E, glutathione and the like. Its related substances, and calcium,
Water-soluble salts of minerals such as iron and phosphorus are components that are preferably formulated nutritionally.

When formulated as foods, confectionery, breads, noodles, cooked rice, seasonings, spices, dairy products, along with carbohydrates such as flour and starch, protein materials such as soy protein, meat and / or fats and oils. , Can be blended in side dishes. In order not to inhibit digestion and absorption of nutrients, it is desirable that the compounding amount does not exceed 10% of the total protein in the food. The powder or granule form is convenient for incorporating the inventive agent into a food material. In this case, coating the surface of the soybean whey fraction with a digestible film composed of a prolamin protein such as glutelin, zein or hordein reduces the hygroscopicity of the fraction and the unpleasant taste of the fraction. This is advantageous for masking. By the way, such a coating process,
It can also be applied to the coating of granules in the tableting process.

[0022]

The trypsin inhibitor, which is considered to be an active ingredient of the agent of the present invention, has an effect of suppressing the occurrence of skin cancer orally without taking much inhibition of nutrient absorption when taken in a small amount per protein. Therefore, by taking this agent daily as a health food or adding it to a food material or finished food, skin cancer can be prevented from occurring.

[0023]

【Example】

Example 1 25 parts by weight of gelatin (the same applies hereinafter), 50 parts of stearic acid, 10 parts of pectin, 400 parts of lactose, 515 parts of potato starch, 10 parts of thiamine, 100 parts of L-ascorbic acid, 100 parts of calcium glycerophosphate and concentration of Experiment 1 After sizing 300 parts of whey powder by wet method, use a tableting machine to make 500 mg per tablet.
Was made into a naked tablet.

The above-mentioned naked tablet is placed in a sugar-coating machine, and is hanged with a shellac / ethanol solution while rotating. Then, the tablet is stabbed with white sugar syrup containing gum arabic and titanium dioxide and polished with wax according to a conventional method. Thus, a sugar-coated tablet of health food having an anti-skin cancer action and a nutrition-enhancing action was obtained.

Example 2 The concentrated whey powder obtained in Experiment 1 was mixed with 100 g of a commercially available yogurt taste drink base at a ratio of 2% to obtain a drink base containing a soybean whey fraction. When this drink base is appropriately mixed with milk, a delicious health drink having a yogurt flavor can be obtained.

Example 3 To 100 parts of the soy whey fraction of Experiment 1, 10 parts of calcium glycerophosphate and 90 parts of starch were added and mixed in a dry air stream. This was put into a fluidized bed granulation dryer, and the operation of spraying and drying a separately prepared 70% ethanol solution of zein in a large number of times was repeated. Finally, the coating treatment was performed until the solvent was completely volatilized. , An 80 mesh impervious particle size was classified and collected.

The above granular products are hard to absorb moisture and do not have unpleasant taste or odor. Therefore, materials for processed cereals such as bread, cake, biscuit, etc., clothing for tempura, various roux materials, hamburger, It is suitable for daily ingestion as a component such as a binder for processed meat products such as sausage, and is useful not only for prevention of skin cancer but also for supplementation of calcium.

[0028]

As described above, the present invention has developed a use of soybean whey, which has been regarded as industrial waste, as an anti-skin cancer drug or health food.
It can contribute to the development of the soybean protein manufacturing industry and maintenance of health.

[Brief description of the drawings]

FIG. 1 is a graph showing the relationship between body weight and food intake during the 14th week of breeding.

FIG. 2 is a graph showing the percentage of tumor mice at 14 weeks after application of initial factors.

FIG. 3 is a graph showing the number of tumors per tumor-bearing mouse.

FIG. 4 is a graph showing the tumor volume of tumor-bearing mice (the above graphs are drawn in comparison with control animals).

──────────────────────────────────────────────────続 き Continued on the front page (58) Field surveyed (Int. Cl. ⁷ , DB name) A61K 35/78 A23L 1/48 BIOSIS (STN) CA (STN) MEDLINE (STN)

Claims (1)

(57) [Claims] An anti-skin cancer agent comprising a soy whey fraction as an active ingredient.