JP3194019B2 - Electrophoresis pattern analyzer - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an electrophoresis pattern analysis apparatus, and more particularly to an improved electrophoresis apparatus for analyzing electrophoresis patterns obtained by electrophoresis together with images for a clinical test up to component concentrations with high accuracy. The present invention relates to an electrophoresis pattern analyzer.

[0002]

2. Description of the Related Art For quantitative analysis of an electrophoresis pattern, an analyzer using an absorbance method, a fluorometric method, a radioimmunoassay, an enzyme immunoassay or the like is used. An absorbance method will be described as a typical example of an electrophoresis pattern analysis apparatus.

When performing quantitative analysis of an electrophoresis pattern using absorbance, a specimen is fractionated by electrophoresis, and optical components are used to measure the concentration of components in the formed electrophoresis pattern. Is transmitted through the electrophoresis pattern and the transmitted light is received, the intensity of the incident light and the transmitted light are determined, and the component concentration is measured according to Lambert-Beer's law using the absorbance, which is the logarithm of the transmittance. And perform quantitative analysis. The developing direction d and the straight line scan to fractionated component in a direction parallel to the electrophoresis pattern 10 on the substrate using an optical means having a slit S _o as shown in the FIG. 9, for example Alb, alpha _1, alpha _2, β, γ
And the like, and the component concentration is measured using the obtained absorbance distribution of the fractionated component to perform quantitative analysis. At this time, the width W _o of the slit S _{o is} the width W of the electrophoresis pattern 10.
_The migration pattern 10 is linearly scanned with a dimension smaller than ₁₀ (Kuroki: Medical Tec).
h-nology, 1130, Vol. 7 No. 13 (1
979)).

This analysis of the electrophoresis pattern is effective in the case of reflection and transmission using a single sensor. However, the image of the fractionated component cannot be accurately recognized. the outer shape of the fractionation components of the loading parameters due to the influence of various factors of the loading Patameta including religious service mobility is irregularly formed, moreover, it is detected across all dimensions region to the width W _o of the electrophoresis pattern in However, the measurement accuracy of the component concentration may be reduced, which is not preferable.

[0005]

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems of the prior art.
In analyzing the electrophoresis pattern, it can recognize the image of the fractionation component of the electrophoresis pattern, output the image and the component concentration simultaneously, effectively prevent the deterioration of the measurement accuracy of the component concentration, diagnose and judge clinical tests, etc. An object of the present invention is to provide an analysis of an electrophoresis pattern which can improve the function of the electrophoresis.

[0006]

In order to achieve the above object, according to the present invention, the electrophoresis pattern is analyzed by receiving light such as reflected light, transmitted light or fluorescence from the electrophoresis pattern obtained by electrophoresis. For analyzing the electrophoresis pattern, for irradiating light on one line with a width wider than the width of the electrophoresis pattern, and receiving reflected light, transmitted light or fluorescent light in conjunction with the incident light part A detection unit for detecting an image signal of the migration pattern by linearly scanning with an image sensor in a direction parallel to a developing direction of the migration pattern, and an analysis unit for analyzing the migration pattern from the detected image signal. , Are provided.

[0007]

In this manner, the fraction components can be distributed over the width and development directions of the electrophoresis pattern formed on the support over the entire width and development dimensions by using optical means. The image can be accurately recognized, and the image and the component density can be output simultaneously. Therefore, it is possible to avoid lowering the measurement accuracy of the component concentration, and it is possible to improve functions such as diagnosis and determination of a clinical test.

[0008]

Embodiments of the present invention will be described below with reference to the drawings.

FIG. 1 is an explanatory view showing an embodiment of the present invention in which an image sensor linearly scans in a direction parallel to the developing direction of an electrophoretic pattern, and FIG. 2 shows an image sensor in a direction perpendicular to the developing direction. FIG. 3 is an explanatory view of linear scanning, FIG. 3 is an explanatory view of linear scanning by an image sensor in a direction parallel to the developing direction and at right angles to the image sensor, FIG. 4 is a perspective view of a main part showing the arrangement of the optical means in FIG. FIG. 6 is a perspective view of an essential part showing an arrangement of the optical means in FIG. 2, FIG. 6 is a circuit diagram of an electrophoresis pattern analyzing apparatus showing one embodiment of the present invention, FIG. 7 is a flow diagram of the arithmetic processing circuit, FIG. Is an explanatory diagram of a display screen of the display device, FIG. 9 is an explanatory diagram showing a comparison between an absorbance distribution curve of the present invention and a conventional technology, and FIG. 10 is an explanatory diagram showing an image signal of FIG.

[0010] In FIG. 1, the optical unit electrophoresis pattern 10 obtained by electrophoresis performing optical processing by a row line with a wide dimension W than the width W ₁₀ of the electrophoresis pattern 10 1
2, a direction A parallel to the developing direction d of the migration pattern 10
Is scanned linearly. The luminous flux is directed in the normal direction on the surface of the electrophoresis pattern 10, and 14a indicates the path of the scanning line.
Thus, the image signal of the migration pattern 10 is detected.

[0011] In FIG. 2, optical means performs the optical processing by a row line with a long dimension L than the developed length L ₁₀ is electrophoresis pattern 10 obtained in the same manner as described above in the deployment direction d of the electrophoresis pattern 10 12 Is used to perform linear scanning in the direction B perpendicular to the developing direction d of the electrophoresis pattern 10.
Reference numeral 14b indicates the path of the scanning line. Thus, the image signal of the migration pattern 10 is detected.

In FIG. 3, the migration pattern 10 obtained in the same manner as described above is scanned in the direction A parallel to the developing direction d of the migration pattern 10 by using the first optical means 12a as shown in FIG. Linear scanning is performed along the line path 14a, and the second optical unit 1 is moved as shown in FIG.
Using the scanning line 2b, the scanning pattern 10 is linearly scanned in a direction B perpendicular to the developing direction d of the electrophoresis pattern 10 with a scanning line path 14b. In this way, the image signal of the migration pattern 10 is detected from both directions with the first image signal and the second image signal in the first optical unit 12a and the second optical unit 12b.

In FIG. 4, the electrophoresis patterns 10 are formed in multiple rows on a support 16, and the electrophoresis patterns 1 in a single row are formed.
In order to detect 0, openable and closable shielding plates 18 are opened on the upper surface of the support 16. The optical means 12 includes an incident light portion for irradiating light and a light receiving portion for receiving reflected light, transmitted light or fluorescent light in conjunction with the incident light portion. The incident light portion includes a light source of irradiation light (not shown), a surface mirror, a plano-convex lens, a filter, a slit Sa ', and the like, and the light receiving portion includes a slit Sa, a lens, a filter, an image sensor described later, and the like. The width of each of the slits Sa and Sa ′ is wider than the width of the migration pattern 10. In this manner, the irradiation light has a width larger than the width of the fraction component of the migration pattern 10 in the slit S.
The transmitted light that is incident, irradiated, passes through the electrophoresis pattern 10 and exits through the slit Sa through the a ′ is formed as a two-dimensional light image on one line of the image sensor, and converted into an electric signal. The converted image signal of the migration pattern 10 is detected. The image sensor has a bit number suitable for the resolution, and an image element such as a CCD is suitable. Further, the optical unit 12 is also used as the first optical unit 12a.

In FIG. 5, optical means 12 is provided as in FIG. The slit Sb 'is for the incident light part, and the slit Sb is for the light receiving part. Slit S
The widths of b and Sb 'are longer than the developed length of the migration pattern 10. In this manner, the irradiation light is incident and radiated through the slit Sb 'with a dimension longer than the developed length of the fraction components of the migration pattern 10, is transmitted through the migration pattern 10, and reaches the light receiving sensor through the slit Sb. The transmitted light is imaged on one line of the image sensor as a two-dimensional light image, converted into an electric signal, and the image signal of the migration pattern 10 is detected. Further, the optical unit 12 is also used as the second optical unit 12b.

In FIG. 6, an electrophoresis pattern analyzing apparatus 100 is roughly divided into an optical unit 12 for detecting an image signal of the electrophoresis pattern 10 and an electrophoresis pattern 10 analyzed from the image signal detected by the optical unit 12. For analysis.

The optical circuit of the optical means 12 is simplified, and includes the light source 13 for the irradiation light, the S 'of the incident light portion, the migration pattern 10 formed on the support 16, the slit S of the light receiving portion, and the detection portion. It comprises an image sensor 15 and the like.
Electrophoresis pattern 10 detected by image sensor 15
Are output to the analyzer 20 as electric signals.

In the analysis section 20, the image sensor 1
5 is amplified by an amplifier, and the amplified electric signal is supplied to an analog / digital conversion circuit 22.
And converted into digital data. The image signal converted to digital data is stored in a memory 24, and the data recorded in the memory 24 is processed by an arithmetic processing circuit 26.
And sent to the display device 32, the output printer 34, and the like through the interface circuit 28. The overall control of such a series of signal processing is performed by the control circuit 30. The image data input to the memory 24 corresponds to the absorbance of a fraction obtained by sequentially scanning the migration pattern 10.

FIG. 7 shows an example of the arithmetic processing in the arithmetic processing circuit 26. Each absorbance data at the scanning position obtained by sequentially linearly scanning the direction perpendicular to and / or parallel to the developing direction of the migration pattern 10 is read from the memory 24, the absorbance data is added, and the added value is read into the memory. The above addition value at the next scanning position is read into the memory, and these procedures are repeated over the entire scanning area, and the integrated value of the absorbance is obtained and newly written into the memory 24. Further migration pattern 1
By dividing the partial integral of the absorbance obtained by the sequential scanning in the zero fractionation area by the overall integral, the component concentration of the fraction can be output. Then, an image of the migration pattern 10 is also output and can be recognized at the same time in accordance with the above-described arithmetic processing procedure.

Referring to FIG. 8, the display device 32 of the analysis unit 20
Is displayed, and the component density distribution (a) and the recognition image (b) of the fraction obtained from the image signal detected by linearly scanning in the direction parallel to the developing direction of the migration pattern 10 as the display screen are shown. Are output at the same time, and are not individually output as in the related art, so that functions such as diagnosis and determination of a clinical test can be improved.

[0020] In FIG. 9, the slit electrophoresis pattern 10 has a wide dimension W than the width W ₁₀ of the electrophoresis pattern 10 S
Using optical means 12 having, showed an absorbance distribution curve obtained from an image signal detected by the linear scanned by the image sensor in parallel to the development direction d direction, narrower dimension than the width W ₁₀ is prior art is compared with the absorbance distribution curve obtained by the slit S _o with W _o. Since it detected for all the patterns of the width W ₁₀ of the electrophoresis pattern 10 according to the present invention, more accurate absorbance distribution as compared with the prior art, that is, to obtain information of the component concentrations.

FIG. 10 shows an analysis example of an image signal detected by the scanning method of the migration pattern 10 shown in FIG. For the sake of simplicity, in FIG. 10, the optical means 12 is at the scanning positions of X _i , X _j , X _k and
An analysis example of an image signal in a case where the obtained fractions are 1,... 5 is shown.

[0022] in FIG. 10 (b), (c) , in _{(d), P k1, ...} P k5, P j1, ... P j5, P i1, ... P i5 are fractions detected by the scanning position The peak values of the absorbance distribution curve are shown, and in FIG. 10A, P ₁ ,..., P ₅ show the sum of the peak values of each fraction.

The method of analyzing the image signal described above will be described in detail as follows. P ₁ = Σ (P _{01 ＰP i1} , P _j1 , P _{k1 ＰP n1} ) P ₂ = Σ (P _{02 ，} P _i2 , P _j2 , P _{k2 kP n2} ) ………………………… _{.............................. P 5 = Σ (P 05} ... P i5, P j5, P k5 ... P n5) ............................................................ P _n = Σ (P _0n ... P _in , P _jn , P _kn ... P _nn ) where P ₁ ,... P _n , the peak value of the absorbance distribution curve of each fraction (P
₀₁ ... P _nn ) Scanning order, X _o ... X _i , X _j , X _k ... X _n If the image signal of the migration pattern 10 is detected as described above, Even
The required number of scans is repeated and repeated, and the sum of the peak values of the absorbance distribution curves of the fractions detected at each scanning position is obtained, whereby accurate component concentrations of the fractions can be obtained.

[0024]

As described above, according to the present invention,
Even if the shape of the fractionation of the electrophoresis pattern formed on the support presents a distorted outer shape, the width and the development length are measured using optical means over the width and development directions of the electrophoresis pattern. It is possible to accurately recognize the image of the fraction component over all of the size regions, and to simultaneously output the image and the component density. Therefore, it is possible to avoid lowering the measurement accuracy of the component concentration, and to improve functions such as diagnosis and determination of a clinical test.

[Brief description of the drawings]

FIG. 1 is an explanatory diagram showing one embodiment of the present invention, in which an image sensor linearly scans in a direction parallel to a developing direction of an electrophoretic pattern.

FIG. 2 is an explanatory view showing one embodiment of the present invention, in which an image sensor linearly scans in a direction perpendicular to a developing direction of an electrophoresis pattern.

FIG. 3 is an explanatory view showing one embodiment of the present invention in which an image sensor linearly scans in a direction parallel to a developing direction of an electrophoretic pattern and in a direction perpendicular to the direction of development of an electrophoretic pattern.

FIG. 4 is a perspective view of an essential part showing an arrangement of the optical means of FIG. 1;

FIG. 5 is a perspective view of an essential part showing an arrangement of the optical means of FIG. 2;

FIG. 6 is a circuit diagram of an electrophoresis pattern analyzer according to an embodiment of the present invention.

FIG. 7 is a flow diagram of an electrophoresis pattern arithmetic processing circuit showing one embodiment of the present invention.

FIG. 8 is an explanatory diagram of a display screen of an electrophoretic pattern display device according to an embodiment of the present invention.

FIG. 9 is an explanatory diagram showing a comparison between an absorbance distribution curve of the present invention and a conventional technique.

FIG. 10 is an explanatory diagram showing analysis of the image signal of FIG. 2;

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 10 Migration pattern 12 Optical means 12a 1st optical means 12b 2nd optical means 15 Detection part 20 Analysis part 100 Analysis apparatus

──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-61-155732 (JP, A) JP-A-55-164336 (JP, A) JP-A-64-59143 (JP, A) 139461 (JP, U) (58) Fields investigated (Int. Cl. ⁷ , DB name) G01N 27/447 G01N 21/17 G06F 19/00

Claims (3)

(57) [Claims] 1. An electrophoresis pattern analyzer for analyzing a migration pattern by receiving light such as reflected light, transmitted light, or fluorescence from an electrophoresis pattern obtained by electrophoresis, wherein the width of the electrophoresis pattern is larger than the width of the electrophoresis pattern. Also has an incident light part that irradiates light on one line with a wide dimension and a light receiving part that receives reflected light, transmitted light or fluorescent light in conjunction with the incident light part, and has an image parallel to the developing direction of the migration pattern. An electrophoresis pattern analysis device, comprising: a detection unit configured to detect an image signal of an electrophoresis pattern by linearly scanning with a sensor; and an analysis unit configured to analyze the electrophoresis pattern from the detected image signal. 2. An electrophoresis pattern analyzer for analyzing a migration pattern by receiving light, such as reflected light, transmitted light, or fluorescence, from the migration pattern obtained by electrophoresis, comprising: Includes an incident light part that irradiates light on one line with a longer dimension and a light receiving part that receives reflected light, transmitted light, or fluorescence in conjunction with the incident light part, and is perpendicular to the direction of development of the migration pattern. An electrophoresis pattern analysis device, comprising: a detection unit configured to detect an image signal of an electrophoresis pattern by performing a linear scan with an image sensor; and an analysis unit configured to analyze the electrophoresis pattern from the detected image signal. 3. An electrophoresis pattern analyzer for analyzing a migration pattern by receiving light such as reflected light, transmitted light, or fluorescence from the migration pattern obtained by the electrophoresis, wherein the width of the migration pattern is larger than the width of the migration pattern. Also has an incident light portion for irradiating light on one line with a wide dimension, and a first light receiving portion for receiving reflected light, transmitted light or fluorescent light in conjunction with the incident light portion, and a direction parallel to the developing direction of the migration pattern. A first detector for detecting a first image signal of the electrophoresis pattern by linear scanning with an image sensor, an incident light portion for irradiating light on one line with a dimension longer than the developed length of the electrophoresis pattern, and an incident light portion A second light receiving unit that receives reflected light, transmitted light, fluorescence, or the like in conjunction with the image sensor and detects a second image signal of the migration pattern by performing a linear scan with an image sensor in a direction perpendicular to the direction in which the migration pattern is developed. An electrophoresis pattern analysis apparatus, comprising: a second detection unit that performs analysis; and an analysis unit that analyzes an electrophoresis pattern from the detected first and second image signals.