JP2950698B2 - Automatic analyzer with washing function - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an automatic analyzer having a washing function suitable for performing a high-precision analysis of multiple items in a liquid sample such as blood or urine.

[0002]

2. Description of the Related Art For example, an automatic analyzer for analyzing many items among components of a liquid sample such as blood or urine has a washing function of washing a probe for dispensing a reagent or a sample and washing a reaction container. The washing of the probe is usually performed by discharging ion-exchanged water to the washing tank every dispensing operation. The washing of the reaction vessel is performed by discharging the ion-exchanged water into the reaction vessel at the end of the measurement and then sucking it.

Conventionally, in a sample to be analyzed, there are many items which do not affect the next sample even if only the probe or the like is washed with ion-exchanged water without using a detergent or the like. In recent years, higher measurement accuracy has been required for measuring immune-related substances and the like, and the influence of cross contamination on the next sample cannot be ignored. Particularly, recently developed reagents for automatic analyzers contain special reagents, so that it is necessary to further suppress the influence on the next sample.

[0004] There are a wide variety of items to be analyzed as described above and reagents for analyzing the items. For example, lipid-based substances are very effective in acidic detergents. In addition, immune-related H
CG (human chorionic gonadotropin) has a property of easily adsorbing to a sample probe or the like, and a chlorine-based detergent is effective for this. Furthermore, in the analysis method using the latex agglutination method, the latex present in the reagent has a property that it is easily adsorbed to the reaction container or the reagent probe, and it is known that this has a cleaning effect with a surfactant. However, in many automatic analyzers, the time interval of one dispensing operation is only 10 to 20 seconds, so that the washing of the probe is limited to only water washing or washing with one kind of detergent. When performing analysis, it is difficult to suppress the influence on the next sample by washing with water or washing with one kind of detergent. In addition, some items of the same type of detergent may not be effective unless they are at a high concentration.
Some items are effective even at low concentrations.

In order to avoid cross-contamination between items, Technical Data ACA Sheet No. 48, "Avoidance of Cross-Contamination by High Carinone Using Cleaning Program" (Measuring Instruments Division, Hitachi, Ltd., 1992) (February) was developed (this is referred to as a first prior art). In this cleaning program, the detergent to be used is put in the reagent container in advance, set on the reagent disk,
The items to be washed, the position of the detergent to be used on the reagent disk, the amount of the washing solution and the washing place (reaction container, reagent probe, etc.) are registered from the keyboard of the automatic analyzer. In this washing program, a reagent for an item registered for analysis is dispensed by a probe of a dispensing mechanism, and then the dispensing mechanism rotates and aspirates the detergent in a chamber of the reagent container to the probe. Further, the dispensing mechanism rotates and discharges the detergent into the cleaning tank, and then the cleaning water (ion exchange water) supplied from the water tank via the tube is discharged from the probe.

As another prior art relating to this type of automatic analyzer, as described in Japanese Patent Application Laid-Open No. 63-88461, water, detergent, and reagent blank are used in accordance with the combination of measurement items. There is a method of selectively dispensing and adjusting the content into a reaction vessel, supplying it to a measurement flow path in which an item is measured, and cleaning the measurement flow path. However, the detergent is one type and does not have a configuration in which a detergent can be selected for each item (this is referred to as a second conventional technique).

[0007]

The cleaning program according to the first prior art has a problem that it takes time and effort to register a cleaning method for each item, and a mistake is likely to occur in the installation of a detergent. In particular, the 21st Journal of Japan Society of Clinical Laboratory Automation (198
(9, vol. 14, p. 384-p. 385) Effective detergents for items have been studied in the abstracts 106 and 107, but no detergent has been established that can effectively wash new items in recent years. In some cases, it takes a long time to select a detergent.

[0008] In general, many detergents are stable in a high-concentration liquid, and some detergents are stable in a solid state such as a powder or a tablet. If used after the elapse of the period, denaturation or deterioration often occurs and the cleaning effect is lost. On the other hand, in the first prior art, it is assumed that the detergent is already used after being diluted or dissolved, and since the time after dilution or dissolution cannot be grasped, when the detergent is denatured or deteriorated, Reduces the cleaning effect.

Further, since some of the places where the reagent containers of the reagent disks should be placed in order to use the effective detergent for the items are occupied by the detergent containers, if the types of detergents are increased, the processing time and the like are limited. However, there is a disadvantage that the number of items that can be analyzed at the same time decreases accordingly.

In this washing program, the sample probe cannot be washed with a detergent, but can only be washed with water. Therefore, it is difficult to analyze a substance which easily adsorbs to the sample probe, such as HCG (human chorionic gonadotropin), by an automatic analyzer.

Further, in the second prior art, only one kind of detergent can be used, and an effective detergent cannot be selected for each item. Therefore, it is difficult to suppress the influence of cross-contamination on the next sample when performing multi-item analysis.

A first object of the present invention is to provide an automatic analyzer having a washing function which can carry out a high-precision analysis by suppressing carry-over of a sample or a reagent to a next sample in an automatic analyzer for analyzing multiple items. It is to provide.

A second object of the present invention is to provide an automatic analyzer for analyzing a large number of items, in which analysis and washing can be performed by simple operations without reducing the number of items that can be analyzed simultaneously, and
An object of the present invention is to provide an automatic analyzer with a washing function capable of performing high-precision analysis while suppressing carryover of a sample or a reagent to a next sample.

[0014]

Means for Solving the Problems To achieve the above object,
Therefore, the present invention is configured as follows. (1) It has a reagent probe that can absorb and
After reacting the drug in the reaction vessel, the reaction
In an automatic analyzer for analyzing a liquid, (a) a plurality of detergent accommodating sections accommodating a detergent liquid are kept in a rotating shaft.
(B) washing means respectively attached to the plurality of detergent accommodating portions.
Agent identification code for identification, and reading means for reading (c) said identification code, reading of the identification code by (d) said reading means
Control unit for managing the plurality of detergent storage units based on the
The control unit is configured to control the reagent probe after use.
Specific reagents that require detergent cleaning for
After dispensing from the reagent container to
Until the reagent dispensing of the above, the above identification on the detergent positioning device
The above identification code indicates that this is a detergent liquid for reagents.
The reagent solution is sucked by the reagent probe and the suction
The discharged detergent solution is discharged from the reagent probe to the discharge position.
Let

(2) Preferably, in the above (1),
High-concentration detergent or solid detergent is previously stored in each of the above-mentioned detergent storage sections.
The reagent probe is contained before starting use of the detergent.
Form a detergent solution by adding a diluting or dissolving solution
To achieve .

(3) Preferably, in the above (1),
And is not formed integrally with the reagent container for each analysis item.
A plurality of reagent container units,
Each is provided in each of the reagent container units, and
The means is disk-shaped, and each reagent container unit is
The arrangement direction of the agent container and the reagent container is the radius of the holding means.
It is arranged along the direction .

(4) Preferably, in the above (1),
The holding means moves each detergent accommodating portion to the detergent suction position.
Positioning multiple reagent storage units in a ring
A concentric circular disk with the reagent positioning device
I do .

(5) A suction device is provided for the reagent container and the detergent container.
Equipped with a positioning device for positioning the reagent probe.
Dispensed with more dispensed reagents and sample probe
The sample in the reaction vessel reacts with the sample
In an automatic analyzer for analyzing, of the rotary shaft a plurality of detergent storage portion for storing (a) a detergent solution or
(B) washing means respectively attached to the plurality of detergent accommodating portions.
Agent identification code for identification, and reading means for reading (c) said identification code, reading of the identification code by (d) said reading means
Control unit for managing the plurality of detergent storage units based on the
The control unit includes a detergent liquid storage for receiving the detergent liquid.
On the positioning device with the reagent probe relative to the depot
Identified from the detergent identification code above
The dispensed detergent solution is then dispensed, and then the dispensed detergent solution is stored.
The detergent liquid in the detention room is sucked into the sample probe,
Discharge the detergent liquid from this sample probe to the outlet
By doing so, the reagent probe and sample probe
Wash with the above detergent solution.

[0019]

[0020]

[0021]

[0022]

[0023]

[0024]

[0025]

[0026]

[0027]

[0028]

[0029]

According to the present invention constructed as described above, the reagent
After dispensing the reagent with the probe, until the next reagent dispensing
Then, aspirate the detergent solution with the reagent probe used, and
Discharge the liquid to the discharge position. At this time, on the detergent positioning device
Aspirate and discharge the above specified reagent detergent solution with a reagent probe
This means that the reagents to be dispensed must be washed with detergent after use.
It is possible to handle even specific reagents that are necessary.
Reliable cleaning of reagent probe using effective detergent
Can be performed. Therefore, when performing analysis of multiple items , carryover due to cross contamination to the next sample is suppressed , and highly accurate analysis becomes possible.

Further , the same analysis item is stored in the reagent positioning device.
Reagent storage and detergent storage corresponding to each other are integrally formed
Then, by arranging a plurality of reagent container units, a detergent container unit for each item may be used as a detergent capable of effectively washing the probe for each item,
There is no need to select one detergent from among various types of detergents. Therefore, complicated operations as in the case of the first prior art, that is, registration from the keyboard is not required, and there is no mistake in installation of the detergent, thereby facilitating the operation. In addition, since the above-mentioned reagent units contain containers for storing reagents and detergents corresponding to the respective items, some of the places where the reagent containers are placed are not occupied by the detergent containers, and the number of simultaneous analysis items is reduced. Nothing to do.

Also, it is stable in a state of a high concentration liquid or solid.
If a detergent is used, avoid denaturation and deterioration.
As it is in the detergent container of the reagent container unit.
Place, At the time of the start of the analysis, dilution Yai such diluent
Dissolved solution such as on-exchange water is supplied using the dispensing mechanism, and
Used after adjusting the concentration by diluting or dissolving the detergent
You . These diluents and lysates are used in the reagent container unit.
It can be stored in the storage unit or
It may be stored in a separate container other than the container unit.

[0032] The identification code (display hand) attached to the detergent accommodating room.
Read by a reader, and after dilution or dissolution of detergent.
By managing the time after the solution, based on the management information
In addition, it is easy to recognize and manage the state of each detergent for each item.

Further , the washing on the positioning device with the reagent probe is performed.
Dispense the detergent solution from the detergent container and sump the dispensed detergent solution
Suction into the sample probe and discharge from the sample probe
By discharging the detergent solution to the
Both probes of the sample probe can be
Washed.

Further, a detergent liquid storage chamber is disposed adjacent to the discharge section.
In addition, an on-off valve is provided on the outflow path of the detergent liquid storage chamber.
Detergent is sucked by the reagent probe and the detergent solution storage chamber
After the detergent is discharged, the detergent is kept in the detergent solution storage room for a certain period of time.
You can use this retained detergent as a sample
It can be used for cleaning the probe. And the reagent
Cleans both lobe and sample probes
Thereafter, the on-off valve is opened to discharge the detergent liquid.

[0035]

[0036]

[0037]

[0038]

[0039]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS A first embodiment of an automatic analyzer with a washing function according to the present invention will be described with reference to FIGS. First, the configuration of the automatic analyzer with a washing function of the present embodiment will be described. FIG. 1 is an overall view of an automatic analyzer with a washing function of the present embodiment, and FIG. 2 is a view showing a main part thereof, including a reaction disk, a sample disk, a reagent disk,
It is a figure which shows a dispensing mechanism etc. 1 and 2, 1
Is a disk-shaped reaction disk.
Is rotationally driven by the rotational drive mechanism 1A. A large number of reaction vessels 2 are arranged on the outer periphery of the reaction disk 1. Among them, a reference liquid reaction container and a reagent blank reaction container (described later) are arranged at regular intervals. The reaction disk 1 can be rotated by one pitch at a time by the rotary drive mechanism 1A, or can be rotated at a time by a specified number of pitches up to a predetermined position. Further, the reaction disk 1 is immersed in a reaction thermostat 3, whereby the reaction vessel 2 is maintained at a predetermined temperature, for example, 37 ° C. This automatic analyzer is of a batch type, and when one measurement is completed, the entire reaction vessel 2 is replaced. Further, a cleaning mechanism 30 for cleaning a refreshing component or an excessive reagent component contained in the reaction container 2 is disposed near the reaction disk 1.

A disk-shaped sample disk 4 and a reagent disk 5 are provided close to the reaction disk 1. These are rotationally driven synchronously around the same rotary shaft 6 by a rotary drive mechanism 4A. The sample disk 4 has a large number of sample containers 7 each containing a sample, and the reagent disk 5 has a large number of reagent container units 8 in which several types of reagents and detergents are grouped for each item as described later. Is arranged. These sample container 7 and reagent container unit 8 can be attached and detached in accordance with the items to be analyzed. In FIG. 2, the sample disk 4 is placed inside,
Although the reagent disk 5 is provided outside, the reagent disk 5 may be provided inside and the sample disk 4 may be provided outside.

The sample container 7 and the reagent container unit 8 can also be rotated one pitch at a time around the drive shaft 6 by the rotary drive mechanism 4A, or can be rotated at once at a specified pitch number up to a predetermined position. Further, the reagent disk 5 is housed in a reagent cool tank 9, whereby the reagent container unit 8 is maintained at a predetermined temperature.

A dispensing mechanism 10 is provided between the reaction disk 1 and the reagent disk 5, and the dispensing mechanism 10 stores the above-described sample, reagent, detergent, and a reference solution described later in a sample container 7
A probe 11 for dispensing from the reagent container unit 8 to the reaction container 2, and an arm 12 to which the probe 11 is attached at the tip and rotates about a rotation shaft 13.
The probe 11 is a dispensing control unit 92 (FIG. 1) having a built-in pump.
The sample, the reagent, the detergent, etc. are sucked by the action of (1), moved to the dispensing position 14 by the rotation of the arm 12, and discharged to the reaction vessel 2 at this position. At this time, the sample container 7 and the reagent container unit 8 are rotatably moved by the sample disk 4 and the reagent disk 6 and can be stopped on the movement locus of the probe 11. Further, the probe 11 can discharge cleaning water such as ion-exchanged water supplied from the outside via a tube (not shown). This control is also performed by the dispensing control unit 92.

A washing tank 15 for washing the probe 11 is provided on the movement trajectory of the probe 11 between the reaction disk 1 and the reagent disk 5. This washing tank 15
Is used to discharge the detergent sucked by the probe 11 as described later. Cleaning water is supplied to the probe 11 from a cleaning water supply unit (not shown) separately from the detergent, and the inside and outside of the probe 11 are cleaned by discharging the cleaning water from the probe 11 to the cleaning tank 15. .

A common reagent container 16 is provided at a position facing the washing tank 15 with respect to the rotating shaft 13 on the movement locus of the probe 11. The common reagent container 16 is divided into a substrate solution container 16a and a buffer solution container 16b, and contains a substrate solution and a buffer solution, respectively. These substrate solution and buffer solution are for causing a color reaction to occur in a sample reacted as a fluorescent expression reagent (common reagent).

The photometer 17 is a multi-wavelength simultaneous photometry type having a plurality of detectors. The reaction vessel located at the photometry position 18 on the reaction disk 1 opposite to the reaction vessel 2 emits a light beam from a light source lamp 19. 20. Excitation light of a predetermined wavelength is irradiated from above or from the bottom of the reaction vessel 2, fluorescence generated in the sample solution is taken out from the side of the reaction vessel, a predetermined wavelength is selected and detected by the photomultiplier tube 21 which is a photoelectric detector. . In FIG. 2, the configuration of the photometer 17 is schematically shown.

Returning to FIG. 1, the operation of each device is controlled by the microcomputer 90. The rotation drive mechanisms 1A and 4A for the reaction disk 1, sample disk 4 and reagent disk 5 are controlled by a microcomputer 90 via an interface 91. Similarly, the probe 1 in the reaction thermostat 3, the reagent cooler 9, and the dispensing mechanism 10
The dispensing control unit 92 for controlling the ascending / descending and rotating movement of the 1 and the suction and discharge of a syringe (not shown) and the washing water pump 93 of the washing mechanism 30 are also controlled by the microcomputer 90 via the interface 91. The signal from the photometer 17 is supplied to the A / D converter 94 and the interface 91.
Through the microcomputer 90. A CRT 95 and a printer 96 are for displaying processed data, and a keyboard 97 is used by an operator for inputting predetermined commands and analysis conditions, and is provided with a floppy disk 98 as storage means and an RA.
M99 is used as a means for reading the system or for storing analysis data collectively. The microcomputer 90, the interface 91, the dispensing control unit 92,
And the above-mentioned rotation drive mechanisms 1A and 4A constitute a control unit.

Next, an example of the configuration of the reagent container unit will be described. As shown in FIG. 3A, the reagent container unit 8 has a structure in which reagent storage units 81 to 84 and a detergent storage unit 85 that respectively store a reagent and a detergent for a specific analysis item are integrated. (Hereinafter, this accommodating part is referred to as a chamber). The upper part of the reagent container unit 8 is sealed with a vinyl sheet 86 so that it does not spill even if it falls down. When dispensing the reagent and the detergent, the probe 11 is connected to the vinyl sheet 8 as shown in FIG.
6 to enter the chambers 81-85. Also,
In FIG. 3B, the detergent 87 in the chamber 85 is a solid (tablet), and a certain amount of a dissolving agent such as ion-exchanged water is injected into the chamber 85 by the probe 11 and used after being dissolved in advance. This dissolving agent may be stored in one of the chambers 81 to 84, or may be stored in a separate container.

As shown in FIG. 3C, a bar code label 88 (identification code) as a display means for displaying the type of reagent or detergent is attached to the side of each reagent container unit 8. When the unit 8 is set on the reagent disk 5, it is read by a barcode reader (not shown) and stored in the microcomputer 90. As a result, the start time of use of the reagent, the amount of use of the reagent and the detergent, the time after dilution or dissolution of the detergent, and the like are recognized and managed.

In the above, the detergent is solid,
A highly concentrated liquid may be used. In this case as well, probe 1
It is used after being diluted with a certain amount of a diluent such as ion-exchanged water according to 1. The reason why the detergent is stored in a solid or high-concentration liquid state is that many of the detergents are more stable, so that denaturation and deterioration are avoided.
Of course, a stable detergent may be stored as it is even if it is not in a solid or high-concentration liquid state. Further, the types of reagents are not limited to four as shown in FIG. 3, but can be increased or decreased according to the analysis items.

When the cleaning effect is improved by mixing a plurality of detergents, or when a detergent that becomes unstable after mixing is used, a plurality of detergents are stored in a reagent container unit, and these are stored in a reagent container unit. The mixture may be used after being mixed by the probe 11. Further, when a reagent or a detergent necessary for one item cannot be accommodated in one reagent container unit, a plurality of reagent container units may be used.

Next, the basic operation will be described. A predetermined amount of sample is sampled from the sample container 7 on the sample disk 4 into the probe 1.
The liquid is aspirated at 1 and quantified, and is discharged to the reaction container 2 at the designated dispensing position 14 on the reaction disk 1. Then, after the sample is discharged, the probe 11 is washed by a method described later, then the reaction disk 1 is rotated by one pitch, and the sample disk 4 is rotated to the next position to be sucked. By repeating this operation sequentially, first, a required number of samples are transferred and dispensed into the reaction vessel 2.
Next, the probe 11 similarly aspirates from the reagent chamber of the reagent container unit 8 on the reagent disk 5 and
After the reagent is discharged, the probe 11 is washed by a method described later, and this operation is sequentially repeated to sequentially transfer and dispense the reagent. If the item requires a plurality of reagents, the same cycle of reagent transfer and dispensing as described above is repeated.
In this way, the designated rotation of the reaction disk 1, the sample disk 4, and the reagent disk 5 is performed, and the sample and the reagent are dispensed into the reaction container 2.

At the dispensing position 14, the sample sequentially dispensed into the reaction container 2 and a reagent composed of, for example, an antibody coated on magnetic particles, start a reaction in the reaction container 2.
The reaction proceeds until the reaction vessel 2 rotates on the reaction disk 1 one pitch at a time and reaches the washing mechanism 30, and the measurement target component contained in the sample binds to the antibody on the magnetic particles. In the washing mechanism 30, the plurality of reaction vessels 2 are simultaneously washed by the plurality of washing probes, and the fresh components and excess reagent components contained in the sample are discharged out of the reaction vessel 2, and the magnetic particles of the reaction solid phase are removed. It remains in the reaction vessel 2.

When the reaction vessel 2 after the washing is stepped to the reagent dispensing position 18, the fluorescent expression reagent (common reagent) is sucked by the probe 11 from the common reagent vessel 16 and dispensed into the reaction vessel 2. . Thereby, the color reaction proceeds. Then, the reaction vessel 2 is successively positioned at the photometric position 18 by the transfer operation of the reaction disk 1, and the photometer 17
The fluorescence intensity of each sample that has caused a color reaction is measured. The output of the photometer 17 is converted into a digital signal by the A / D converter 94, and is log-converted and input to the microcomputer 90 via the interface 91. Based on the signal input to the microcomputer 90, the concentration of the measurement target in the measurement sample is calculated,
The calculated density data is displayed on the screen of the CRT 23 via the interface 91 or the printer 9
6 is printed out. The result is stored in the floppy disk 98 and the RAM 99.

Next, the operation of the immunoassay using the automatic analyzer with a washing function of this embodiment will be described with reference to FIG.
As shown in FIG. 4, when the analysis operation of the automatic analyzer is started in step 101, the general sample is dispensed from the sample container 7 to the reaction container 2 by the probe 11 of the dispensing mechanism 10 in step 102. This operation will be described later with reference to FIG.

Thereafter, in step 103, the reagent is dispensed from the chamber of the reagent container unit 8 containing the reagent to the reaction container 2. This operation will be described later with reference to FIG. Then, in step 104, the reaction vessel 2
Is kept at a predetermined temperature of, for example, 37 ° C. (incubation), and the reaction proceeds. The reaction container 2 reaches the position of the washing mechanism 30 after a predetermined time (for example, 30 minutes) from the start of the reaction. The liquid containing the unreacted substance such as the reagent component is discharged, and only the magnetic particles of the reaction solid phase remain in the reaction vessel 2 (step 105).

On the other hand, the reference liquid is dispensed from the unillustrated container to the reaction container for the reference liquid in the row of the reaction containers 2 by the probe 11 of the dispensing mechanism 10 (step 106), and the fluorescence developing reagent is (Common reagent) is dispensed into a reagent blank reaction container (Step 107), and a common reagent is dispensed from the common reagent container 16 into the general sample reaction container 2 washed in Step 105 (Step 1).
08). As an example of the substrate of the fluorescent expression reagent, 4-methylumbelliferyl phosphate (4-methyluumbellifery)
lphosphate) solution or the like is used. This substrate generates a fluorescent substance, 4-methylumbelliferone, by causing an enzymatic reaction using alkaline phosphatase as an enzyme.

In step 109, it is determined whether or not the reaction container 2 positioned at the photometric position 18 is a reference liquid reaction container.
Go to 10. In step 110, similarly to step 109, it is determined whether or not the container is a reagent blank reaction container.
Proceed to. In step 111, the fluorescence intensity of the reaction liquid of the general sample is measured by the photometer 17.

If it is determined in step 109 that the reference liquid reaction container has reached the photometry position, the process proceeds from step 109 to step 112, and
At 12, the photometer 17 measures the fluorescence intensity of the reference solution. Then, in step 113, a correction coefficient is calculated from a preset value based on the measured value of the reference liquid.

Further, if it is determined in step 110 that the reagent blank reaction container has reached the photometric position, the process proceeds from step 110 to step 114, where the photometer 17 measures the fluorescence intensity of the reagent blank. Measured. The fluorescence intensity of this reagent blank is not affected by the antigen-antibody reaction complex, and in the next step 115, it is determined whether or not the fluorescence intensity measurement value is larger than a previously stored allowable value. If the fluorescence intensity measurement value of the reagent blank is larger than the allowable value, an alarm indicating that the fluorescent expression reagent has deteriorated is displayed on the CRT screen in step 116, and this is printed out by the printer in step 119. You.

If the measured value of the fluorescence intensity of the reagent blank is smaller than the allowable value in step 115, the process proceeds to step 117. In step 117, the fluorescence intensity measurement value of the general sample which is subsequently subjected to fluorescence photometry is corrected by the fluorescence intensity measurement value of the reagent blank.

In step 118, step 11
The corresponding component concentration is calculated from the correction count calculated in step 3 and the fluorescence intensity measurement value of the general sample corrected in step 117 and the calibration curve stored in advance, and step 11 is performed.
At 9, the analysis value of each sample is output to the printer. Then, when the analysis operation of all the samples is completed, the operation of the automatic analyzer is stopped (Step 120).

Next, the dispensing operation of the general sample in step 102 of FIG. 4 will be described with reference to FIG. When the dispensing operation of the general sample is started (Step 201), in Step 202, the probe 11 is rotated and moved to the position of the washing tank 15, and the ion-exchanged water is discharged and sufficiently washed. Next, proceeding to step 203, the probe 11 is rotated to the position of the sample container 7 for the sample to be dispensed. And
In step 204, it is determined whether or not the amount of the sample is sufficient, for example, by detecting the liquid level of the capacitance type. If it is determined that the amount of the sample is not sufficient, the dispensing operation is stopped in step 205, Proceed to step 212.

If it is determined in step 204 that the amount of the sample is sufficient, the process proceeds to step 206, where the sample necessary for the analysis of the item is stored in the probe 11 from the sample container 7.
Is sucked in. Next, the probe 11 is rotated as it is from the position of the sample container 7 on the sample disk 4 to the discharge position of the reaction container 2 on the reaction disk 1 (step 207), and the sucked sample is discharged to the reaction container 2 (step 207). Step 2
08). Then, in the next step 209, whether or not the analysis of the item requires the detergent cleaning of the probe 11 is determined based on the analysis parameter specific to the item stored in the microcomputer 90 in advance. If it is determined that detergent cleaning is not necessary, the process proceeds to step 212.

If it is determined in step 209 that detergent cleaning is necessary, the process proceeds to step 210, where the reagent disk 5 is moved from the chamber of the reagent container unit 8 containing the detergent to a position where the detergent can be sucked. And the probe 11 rotates. Then, the probe 11 breaks down the vinyl sheet 86 and descends, and a certain amount of detergent registered by the analysis parameters is sucked. Next, the probe 11 rotates and moves to the position of the cleaning tank 15 as it is, and discharges the detergent (step 211). The suction and discharge of the detergent are performed by a syringe or the like (not shown) provided in the dispensing mechanism 10, and the speed of the suction and discharge is controlled according to the above analysis parameters so as to be the most favorable condition for each item. Is done. After the detergent has been discharged, the probe 11 discharges ion-exchanged water and is sufficiently washed (step 21).
2).

Next, in step 213, it is determined whether or not there is a next sample. If it is determined that there is, the process returns to step 203, where the next sample is dispensed by the probe 11 and the probe 11 is washed. If it is determined in step 213 that there is no next sample, step 2
14, the sample dispensing is completed, and step 10 in FIG.
It proceeds to 3, that is, the reagent dispensing operation.

Next, the dispensing operation of the reagent in step 103 of FIG. 4 will be described with reference to FIG. When the dispensing operation of the reagent is started (step 301), step 302
In, the probe 11 is rotated and moved to the position of the washing tank 15, and the ion-exchanged water is discharged and sufficiently washed. Next, the process proceeds to step 303, where the probe 11 is rotated and moved to the position of the chamber in which the reagent of the reagent container unit 8 to be dispensed is stored. Then, the process proceeds to step 304, where the probe 11 aspirates the reagent necessary for the analysis of the item from the chamber of the reagent container unit 8 containing the reagent. Next, the probe 11 is rotated as it is from the position of the reagent container unit 8 of the reagent disk 5 to the position of discharge of the reaction container 2 of the reaction disk 1 (step 305), and the suction and holding is performed.
The stored reagent is discharged to the reaction container 2 (step 30).
6). Then, in the next step 307, whether or not the analysis of the item requires the detergent cleaning of the probe 11 is determined based on the analysis parameter peculiar to the item stored in the microcomputer 90 in advance. If it is determined that detergent cleaning is not necessary, the process proceeds to step 310.

If it is determined in step 307 that the detergent needs to be washed, the process proceeds to step 308, in which the reagent disk 5 is moved from the chamber of the reagent container unit 8 containing the detergent to a position where the detergent can be sucked. And the probe 11 rotates. Then, a certain amount of detergent registered by the above-mentioned analysis parameters is sucked by the probe 11. Next, the probe 11 rotates and moves to the position of the cleaning tank 15 as it is, and discharges the detergent (Step 309). The suction and discharge speeds of the detergent are controlled in accordance with the above analysis parameters so that the best conditions for each item are obtained, as in the case of the general sample. The ion-exchanged water is discharged from the probe 11 after discharging the detergent, and is sufficiently washed (step 310).

Next, in step 311, it is determined whether or not the next reagent is present. If it is determined that the next reagent is present, the process returns to step 303 to dispense the next reagent by the probe 11 and If cleaning is performed and it is determined in step 311 that there is no next sample, step 3 is performed.
Proceeding to 12, the dispensing operation of the reagent is completed, and the process proceeds to step 104 in FIG. 4, that is, to the heat retention (incubation) at a predetermined temperature.

An experimental example using the automatic analyzer with a washing function of the first embodiment will be described with reference to FIG. As described above, HCG (human chorionic gonadotropin), which is an immune-related item, has a property of easily adsorbing to a probe or the like. This adsorption has a great effect on the next sample when the discharge time of the washing water is about 5 to 10 seconds. For example, when the first sample is a high HCG sample and the probe is washed only with water, the carryover to the next sample is about 20 ppm.

In this experimental example, as shown in FIG. 7, a 0.5% sodium hypochlorite solution as a detergent was placed in chamber 1 of the reagent container unit, and a slurry (solid phase) and ALP (alkaline phosphatase) were placed in chambers 2 and 3 as reagents. ) Labeled antibodies are stored respectively. Then, after sucking and discharging the sample with the probe, 50 microliters of the detergent is sucked from the chamber 1 of the reagent container unit. The probe is rotated and discharged to the cleaning tank as it is, and further, ion-exchanged water is discharged from the inside of the probe to the cleaning tank to perform sufficient cleaning. Thereafter, the reagents in chambers 2 and 3 are dispensed in the second and third transfer dispensing cycles. In this way, the washing with the detergent reduced the carryover to the next sample to 1 to 5 ppm. However, HCG is almost nonexistent in a normal person, but has a considerably high value in a pregnant woman or the like. Therefore, the allowable range of carryover to the next sample needs to be suppressed to 0.1 ppm or less.

Then, the concentration of the sodium hypochlorite solution in the chamber 1 was increased to 4.5%, and the probe was washed in the same manner as described above. As a result, the carry-over amount to the next sample was 0.03 to 0.08.
ppm.

Another experimental example using the automatic analyzer with a washing function of the first embodiment will be described with reference to FIG. In this experimental example, thyroid hormone T4 (thyroxine) was measured. In this experimental example, as shown in FIG. 8, a 4.5% sodium hypochlorite solution as a detergent in chamber 1 of a reagent container unit, a slurry (solid phase) and a biotin-binding anti-source as a reagent in chamber 2, and a reagent in chamber 3 As a salicylate, a biotin-binding antigen as a reagent in chamber 4,
The chamber 5 contains an ALP- labeled antibody as a reagent and the chamber 6 contains an anionic surfactant as a detergent.

When the analysis operation starts, first, ion-exchanged water is supplied to the detergent (4.5% sodium hypochlorite solution) in the chamber 1 of the reagent container unit with a probe to dilute the detergent.
Reduce concentration to 5%. This is because this detergent is unstable at a low concentration and cannot be stored for a long time. Then, the sample was dispensed into the reaction vessel, and in the same manner as in the first experimental example, 60 microliters of the detergent in the chamber 1 was sucked by the probe, and then rotated and discharged as it was into the washing tank, and ion-exchanged water was further added. Discharge from the inside of the probe to the cleaning tank to perform sufficient cleaning.

After dispensing all samples, the slurry (solid phase) in chamber 2 and the biotin-bound antigen in the second transfer dispensing cycle were 250 microliters, and the salicylate in chamber 3 was dispensed in the third transfer dispensing cycle. 50 microliters, 100 microliters of biotin-conjugated antibody in chamber 4 in the fourth transfer and dispensing cycle, and 150 microliters of ALP-labeled antibody in chamber 5 in the fifth transfer and dispensing cycle to the reaction vessel I do. Immediately after the reagent dispensing in the fifth transfer dispensing cycle, the probe is rotated, 70 microliters of the detergent (anionic surfactant) in the chamber 6 is sucked, rotated and moved as it is, and discharged into the washing tank. Further, ion-exchanged water is discharged from the inside of the probe to sufficiently wash the probe.

As a result, when the allowable value of the amount carried over to the next sample is determined to be 0.1 ppm or less from the normal value range, the amount carried over to the next sample by the probe is 0.05 to 0.08 ppm, which is less than the allowable value. Results were obtained.

As described above, according to the present embodiment, the cleaning agent that can be effectively cleaned for each item is suctioned by the probe 11 and discharged to the cleaning tank 15 to perform cleaning, so that the cross-contamination to the next sample is performed. Carryover can be suppressed, and highly accurate analysis can be performed. In addition, since the reagent and the detergent are integrated together corresponding to each item, and this is configured as the reagent container unit 8 and arranged on the reagent disk 5, it is not necessary to select each detergent from among various types of detergents. This eliminates the need for complicated operations such as registering from the keyboard, and does not cause a mistake in the installation of the detergent. Further, unlike the conventional case, some of the places where reagent containers are placed are not occupied by detergent containers, and the number of simultaneous analysis items does not decrease.

When the reagent container unit 8 is placed on the reagent disk 5, the state of the reagent and the detergent can be easily recognized and managed by an identification means such as a barcode.

Further, since the detergent is accommodated in the reagent container unit 8 in a state of a liquid or a solid having a high concentration, denaturation and deterioration can be avoided. Then, at the start of the analysis, a diluent such as a diluent or a solution such as ion-exchanged water can be supplied by the probe 11 to adjust the concentration.

Next, a second embodiment of the automatic analyzer with a washing function according to the present invention will be described with reference to FIG. In this embodiment, as shown in FIG. 9, the reagent disk 5a dedicated to the reagent is mounted on the same rotation shaft 6 as the reagent disk 4.
And a detergent disk 5b dedicated to detergents. A large number of reagent container units 8a in which only reagents are grouped for each item are arranged on the reagent disk 5a, and a large number of detergent containers ( A detergent accommodating portion) 8b is provided. The reagent disk 4, the reagent disk 5a, and the detergent disk 5b are driven to rotate around the rotating shaft 6, but they may be rotated in synchronization with each other or independently without synchronization depending on conditions. Other configurations and operations are the same as those of the first embodiment.

With the configuration as in the present embodiment as described above, a detergent that can be effectively cleaned for an item to be analyzed can be appropriately selected from a large number of detergents for cleaning. In addition, one kind of detergent can be used for many items, and a plurality of detergents can be selected and used for one item. Further, unlike the related art, some of the places where reagent containers are placed are not occupied by detergent containers, and the number of simultaneous analysis items does not decrease.

Next, a third embodiment of the automatic analyzer with a washing function according to the present invention will be described with reference to FIGS. FIG. 10 is a diagram showing a main part of the automatic analyzer with a washing function of the present embodiment. In the present embodiment, as shown in FIG.
The two reagent disks of the reagent disks 50 are provided so as to rotate around separate rotation axes, respectively, and the sample disk 60 is also provided so as to rotate around separate rotation axes. Similarly to the first embodiment, the first reagent disk 40 and the second reagent disk 50 are provided with a large number of reagent container units 41 and 51 in which several types of reagents and detergents are grouped for each item. A large number of sample containers 61 are arranged on the sample disk 60.

Further, a first reagent dispensing mechanism 43 having a first reagent probe 42 and a second reagent having a second reagent probe 52 are attached to the first reagent disk 40, the second reagent disk 50, and the sample disk 60. A reagent dispensing mechanism 53 and a sample dispensing mechanism 63 having a sample probe 62 are provided, respectively. When the probes 42, 52, and 62 rotate, the reaction vessels 71 arranged on the reaction disk 70 are moved. The sample, the reagent, and the detergent can be ejected to the apparatus. Further, a washing tank 54 for washing the second reagent probe 52, the first reagent probe 42 and the sample probe 6
A cleaning tank 44 for cleaning both of them is provided. still,
This automatic analyzer is of a continuous type, not a batch type as in the first embodiment, and the reaction mechanism 71 disposed on the reaction disk 70 is washed by a washing mechanism 73 when a series of measurements by a photometer 72 is completed. And then used again in the next measurement.

The cleaning tank 44 is composed of two tanks 44a and 44b as shown in FIG. 11. An electromagnetic valve 44c as a holding means is provided at the outlet of the tank 44b. In the tank 44b, when the detergent is discharged from the first reagent probe 42 as described later, the electromagnetic valve 44c
Is closed and the detergent is held for a certain period of time. Then, the retained detergent is sucked by the sample probe 62 after dispensing the sample, and thereafter, the electromagnetic valve 44 is opened and the remaining detergent is discharged.

The configuration other than the above is the same as that of the first embodiment, and the basic operation of the automatic analyzer with a cleaning function having the above configuration will be described below. For analytical items that use detergents,
In synchronization with the dispensing operation in which the sample is sucked from the sample container 61 by the sample probe 62 and discharged to the reaction container 71,
The detergent for cleaning the sample probe is sucked from the chamber of the reagent container unit 41 containing the detergent by the first reagent probe 42 and discharged to the tank 44b of the probe cleaning tank 44. At that time, the electromagnetic valve 44c is closed, and the detergent is held in the tank 44b for a certain time. In addition, the first reagent probe 42 moves to the tank 44a, and the ion-exchanged water is discharged and sufficiently washed. In the meantime, the sample probe 62 rotates and moves to the tank 44b, and the held detergent is sucked. Then, the electromagnetic valve 44 is opened and the remaining detergent is discharged, while the sample probe 62 is connected to the tank 4 of the cleaning tank 44.
4a, and the detergent is discharged. Further, ion-exchanged water is discharged, and the sample probe 62 is sufficiently washed. At this time, the discharge amount and the discharge speed of the detergent, the amount of ion-exchanged water, and the like are all controlled by the analysis parameters described above.

When a detergent is used for washing the first reagent probe 42 after dispensing the first reagent, the washing is carried out in the same manner as in the first embodiment using only the tank 44a. The washing of the second reagent probe 52 after dispensing is performed in the washing tank 54 in the same manner.

While the reaction disk 70 rotates counterclockwise in the figure, the sample, the first reagent, and the second reagent are dispensed into the reaction container 71 in an amount necessary for analysis, and the reaction proceeds.
Then, photometry is performed by a photometer 72 to measure the concentration of the target component. Since the present embodiment is of the continuous type as described above, the reaction vessel 71 after one measurement is washed by the washing mechanism 73 and is used again in the next measurement. That is, a plurality of cleaning probes are installed in the cleaning mechanism 73, the reaction solution in the reaction container 71 after the measurement is sucked by the first cleaning probe, and ion-exchanged water is discharged by the next cleaning probe,
This is repeated three times to wash the reaction vessel 71.

The reaction vessel 71 is moved to the cleaning mechanism 73 as described above.
When washing with water is not sufficient, ie, the reaction vessel 7
In the case where the remaining reagents and the like in 1 affect the next sample, the reaction liquid is sucked by the washing mechanism 73 and then the reaction disk 70 is
Rotate quickly to the reagent dispensing position. Then, the probe 52 sucks the detergent from the chamber containing the detergent in the reagent container unit 51 on the second reagent disk 50 and discharges the detergent to the reaction container 71. Further, the reaction disk 70
Rotates, the detergent is first sucked by the first washing probe of the plurality of probes of the washing mechanism 73, and ion-exchanged water is discharged by the next washing probe, and this is repeated three times to wash the reaction vessel 71. The detergent discharged from the probe 52 to the reaction vessel 71 is supplied to the cleaning mechanism 73 as described above.
The reaction disk 70 is not
May be further rotated once to soak the detergent during that time, and then cleaned by the cleaning mechanism 73, thereby further improving the cleaning effect.

An experimental example using the automatic analyzer with a washing function of the third embodiment will be described with reference to FIG. In this experimental example, neutral fat (triglyceride) in serum was measured. In this experimental example, as shown in FIG.
0.3 Mol (hereinafter referred to as M) of citric acid as a detergent in chamber 1 of a reagent container unit installed on a reagent disk;
64 mM Tris-citrate buffer, 26.6 mM magnesium sulfate, 0.22 mM NADH as reagents in chamber 2
(Nicotinamide adenine dinucleotide) and the like, while 19 mM sodium chloride and 0.75 IU (International Unit) glycerol kinase, which is an enzyme, are placed in chamber 1 of the reagent container unit installed on the second reagent disk. (Enzyme solution). Then, a serum serving as a sample is put in a sample container and set on a sample disk, and a sample container unit containing a reagent and a detergent shown in FIG. 12 is set on the first reagent disk and the second reagent disk.

When the analysis operation starts, first, 10 ml of ion-exchanged water is dispensed and dissolved in the chamber 2 because the reagent in the chamber 2 of the reagent container unit set on the first reagent disk is a tablet. . Then, 5 microliters of the sample is aspirated from the sample container with the sample probe, and the sample probe is rotated and discharged to the reaction container. This operation is repeated while rotating the reaction disk one pitch at a time.

In synchronization with this, 160 μl of the detergent is sucked from the chamber 1 of the same reagent container unit by the first reagent probe, and the detergent is rotated to the position of the tank b of the washing tank 44 and discharged. This detergent is held in the tank b for a certain period of time by closing the electromagnetic valve. After the detergent is discharged, ion-exchanged water is discharged from the first reagent probe into the tank 44a to sufficiently clean the tank.

The sample probe, which has been discharged into the reaction vessel, is rotated to the position of the tank 44b, and the retained detergent is dispensed for 7 minutes.
0 microliter is sucked and discharged to the tank 44a. Further, ion-exchanged water is discharged from the sample probe and sufficiently washed.

When the reaction container rotates and reaches the first reagent dispensing position, the first reagent probe sucks 350 microliters of the reagent from the chamber 1 of the reagent container unit on the first reagent disk and discharges the reagent into the reaction container. I do. The first reagent probe performs only water washing at the position of the tank 44a of the washing tank 44. Thereafter, when the reaction container reaches the second reagent dispensing position, 50 microliters of the enzyme solution is sucked from the chamber 1 of the reagent container unit on the second reagent disk by the second reagent probe and discharged into the reaction container. And the reaction proceeds 5
After a minute, the concentration is determined by photometry with a photometer.

As a result, when the allowable value of the carryover amount to the next sample is set to 0.5 mg / deciliter or less, the carryover amount to the next sample by the sample probe in the case of only water washing is about 6 mg / deciliter, which is the allowable value. Exceeded 0.08 by washing the sample probe with detergent.
Milligram / deciliter, which was below the allowable value.

Another experimental example using the automatic analyzer with a washing function of the third embodiment will be described with reference to FIG. In this experimental example, ASO (anti-streptolysin-O) was measured using a latex immunoturbidimetry. In this experimental example, as shown in FIG. 13, bovine albumin, lactose, and phosphate buffer were stored as reagents in chamber 1 of a reagent container unit installed on the first reagent disk, while
A streptolysin-O-sensitized latex suspension as a latex reagent and a phosphate buffer solution are contained in chamber 1 of a reagent container unit provided on a reagent disk, and 0.5% anionic surfactant is contained as a detergent in chamber 2. deep. Then, a reagent and a sample are set at respective locations as in Experimental Example 3.

When the analysis operation starts, first, 3 microliters of the sample is sucked from the sample container by the sample probe, and the sample is rotated and moved, and the sample is discharged to the reaction container. After discharging the sample, the sample probe is cleaned by discharging ion-exchanged water at the position of the tank 44a of the cleaning tank 44. That is, in the case of this sample, only water washing is required. Thereafter, the sample is dispensed while rotating the reaction disk by one pitch, and when the reaction container reaches the first reagent dispensing position, the first reagent probe causes the reagent to be dispensed from the chamber 1 of the reagent container unit on the first reagent disk. Is sucked out and discharged into the reaction vessel. The first reagent probe also performs only water washing similarly to the sample probe.

After dispensing the first reagent, when the reaction disk rotates and the reaction container reaches the second reagent dispensing position, the second reagent probe removes the latex reagent from the chamber 1 of the reagent container unit on the second reagent disk. Aspirate 100 microliters and discharge into reaction vessel. Thereafter, 65 microliters of the detergent is sucked from the chamber 2 of the same reagent container unit and discharged into the washing tank. Further, ion-exchanged water is discharged from the second reagent probe to sufficiently wash. Then, the reaction proceeds, and the concentration is determined by irradiating light having a wavelength of, for example, 700 nm with a photometer.

In this experimental example, the sample and the first reagent are not carried over to the next sample only by washing with water, and the carry over of the latex reagent as the second reagent to the next sample becomes a problem. Therefore, washing with a detergent was performed after dispensing the reagent by the second reagent probe as described above.

As described above, according to the present embodiment, the same effects as those of the first embodiment can be obtained. In addition, both the sample probe 62 and the first reagent probe 42 can be added to the respective items in the cleaning tank 44. It can be washed with a detergent that can be effectively washed. Further, since the detergent sucked by the second reagent probe 52 is further discharged to the reaction container 71 and washed by the washing mechanism 73, the reaction container 71 can also be washed with a detergent that can effectively wash each item.

[0099]

According to the present invention, at least one kind of detergent which can be effectively washed with respect to a specific reagent or analysis item is suctioned and discharged by a probe to perform washing, so that cross contamination to the next sample is performed. Carryover due to the nation can be suppressed, and highly accurate analysis can be performed.

Further, since the reagents and the detergents are integrated together corresponding to each item and are constituted as a reagent container unit, it is not necessary to select one detergent from among various types of detergents, and the operation is easy. Perform analysis. Further, unlike the conventional case, some of the places where reagent containers are placed are not occupied by detergent containers, and the number of simultaneous analysis items does not decrease.

Further, since the identification code is provided in the reagent container unit, the state of the reagent and the detergent can be easily recognized and managed.

Further, since the sample container, the reagent container, and the detergent container are arranged concentrically on dedicated disks rotating around the same rotation axis, one detergent can be used for a plurality of items. Or multiple detergents can be used for a single item.

Further, since the detergent is contained in the reagent container unit in a state of a liquid or a solid having a high concentration, denaturation and deterioration can be avoided. Furthermore, the concentration can be adjusted in the reagent container unit or with a separate diluent or dissolution solution.

For one item, a plurality of detergents can be mixed and used, or a plurality of reagent container units can be used.

When the dispensing mechanism for dispensing a sample and a reagent is different, both probes can be washed with a detergent capable of effectively washing each item by the washing tank.

Further, since the detergent sucked by the probe is further discharged to the reaction container, the reaction container can be cleaned with a detergent effective for each item. Also,
A number of detergent compartments are marked with an identification code for detergent identification.
Read the identification code and wash with a suitable detergent.
If it is configured to manage detergents, the type of detergent
The operation of inputting the type by the operator can be omitted. Further
Wash the sample probe with the appropriate detergent
So that a very small amount of residue remains on the sample probe.
Material and remove trace residues on the sample probe.
Cross contamination between samples caused by presence
This greatly reduces the number of options.

[Brief description of the drawings]

FIG. 1 is a first view of an automatic analyzer with a washing function according to the present invention.
1 is an overall view of an embodiment of FIG.

FIG. 2 is a diagram showing a main part of the automatic analyzer with a washing function of FIG. 1 and showing a reaction disk, a sample disk, a reagent disk, a dispensing mechanism, and the like.

3A is a diagram showing an example of a configuration of a reagent container unit used in the automatic analyzer with a washing function of FIG. 1, and FIG. 3B is a cross-sectional view of the reagent container unit shown in FIG. (C) is a diagram showing a state where a barcode label is attached to the side surface of the reagent container unit shown in (a).

FIG. 4 is a flowchart showing an operation of an immunoassay using the automatic analyzer with a washing function of FIG. 1;

FIG. 5 is a flowchart showing a dispensing operation of the general sample in FIG. 4;

6 is a flowchart showing the dispensing operation of the reagent of FIG.

FIG. 7 is a diagram showing an experimental example using the automatic analyzer with a washing function of FIG. 1;

FIG. 8 is a view showing another experimental example using the automatic analyzer with a washing function of FIG. 1;

FIG. 9 shows a second example of the automatic analyzer with a washing function according to the present invention.
It is a figure showing the principal part of an example of.

FIG. 10 is a view showing a main part of a third embodiment of the automatic analyzer with a washing function according to the present invention.

FIG. 11 is a sectional view of a cleaning tank 44 in FIG.

FIG. 12 is a diagram showing an experimental example using the automatic analyzer with a washing function of FIG.

FIG. 13 is a view showing another experimental example using the automatic analyzer with a washing function of FIG. 10;

[Explanation of symbols]

 Reference Signs List 1 reaction disk 1A rotation drive mechanism 2 reaction vessel 4 sample disk 4A rotation drive mechanism 5 reagent disk 5a reagent disk 5b detergent disk 6 rotation axis 7 sample container 8 sample container unit 8a sample container unit 8b detergent container 10 dispensing mechanism 11 probe 12 Arm 15 Cleaning tank 16 Common reagent container 17 Photometer 30 Cleaning mechanism 40 First reagent disk 41 Reagent container unit 42 First reagent probe 43 First reagent dispensing mechanism 44 Cleaning tank 44a, 44b tank 44c Electromagnetic valve 50 Second reagent disk Reference Signs List 51 reagent container unit 52 second reagent probe 53 second reagent dispensing mechanism 54 cleaning tank 60 sample disk 61 sample container 62 sample probe 63 sample dispensing mechanism 70 reaction disk 71 reaction vessel 72 photometer 73 cleaning mechanism 81, 82, 83 84 and 85 chambers 87 detergent (solid) 88 bar code label 90 microcomputer 91 interfaces 92 minutes Note controller

──────────────────────────────────────────────────続 き Continued on the front page (58) Field surveyed (Int.Cl. ⁶ , DB name) G01N 35/10 G01N 1/00 G01N 35/04

Claims (5)

(57) [Claims] 1. A has the reagent probe reagent capable of sucking and discharging, after reaction of the sample and the reagent in a reaction vessel, in the automatic analyzer for analyzing liquid in the reaction vessel after the reaction, (a) detergent solution The multiple detergent storage sections for storing
(B) washing means respectively attached to the plurality of detergent accommodating portions.
Agent identification code for identification, and reading means for reading (c) said identification code, reading of the identification code by (d) said reading means
Control unit for managing the plurality of detergent storage units based on the
When, with the said control unit, the specific reagents required detergent cleaning of the reagent probes after use, from the reagent container by the reagent probe After dispensed into the reaction vessel, until the next reagent dispensing In the meantime , the identification code indicates that the detergent liquid is for the specific reagent on the detergent positioning device.
The reagent solution is sucked into the reagent probe, and the
The detergent solution was then discharged into a discharge position from the reagent probe
Cleaning function automatic analyzer, characterized in that that. 2. The automatic analyzer with a washing function according to claim 1, wherein each of said detergent accommodating portions has a high-concentration detergent or a solid solution.
Body wash is contained and before starting use of the detergent
By adding diluent or lysate from the reagent probe
An automatic analyzer with a washing function, which forms a detergent solution . 3. The automatic analyzer with a washing function according to claim 1, wherein the analyzer is formed integrally with the reagent container for each analysis item.
And a plurality of reagent container units
Each is provided in each of the reagent container units,
The holding means is disk-shaped, and each of the above-mentioned reagent container units
Means that the arrangement direction of the detergent accommodating section and the reagent accommodating section is
An automatic analyzer with a washing function, which is arranged along the radial direction of the sample . 4. An automatic analyzer with a washing function according to claim 1.
In the above, the holding means moves each detergent accommodating portion to the detergent suction position.
The plurality of reagent storage sections are annular.
Concentric annular disk with reagent positioning device to be arranged
An automatic analyzer with a washing function, comprising: 5. The suction device according to claim 1, wherein the reagent storage section and the detergent storage section are located in a suction device.
Equipped with a positioning device that can be placed
Dispensed reagent and sample dispensed by sample probe
In the reaction vessel and analyze the liquid in the reaction vessel
In an automatic analyzer that, the rotation axis a plurality of detergent storage portion for storing (a) a detergent solution or
(B) washing means respectively attached to the plurality of detergent accommodating portions.
Agent identification code for identification, and reading means for reading (c) said identification code, reading of the identification code by (d) said reading means
Control unit for managing the plurality of detergent storage units based on the
And the control unit includes a detergent liquid storage for receiving the detergent liquid.
Chamber on the positioning device with the reagent probe
Identify from the detergent storage unit with the above-mentioned detergent identification code.
The detergent solution, and then store the dispensed detergent solution
Aspirate the detergent solution in the room into the sample probe, and
Of the detergent liquid from the sample probe to the outlet.
Up the reagent probe and sample probe
An automatic analyzer with a washing function characterized by washing with a detergent solution .