JP2598811B2 - Potato tuber formation inducer and method for inducing the same - Google Patents
Potato tuber formation inducer and method for inducing the sameInfo
- Publication number
- JP2598811B2 JP2598811B2 JP63242432A JP24243288A JP2598811B2 JP 2598811 B2 JP2598811 B2 JP 2598811B2 JP 63242432 A JP63242432 A JP 63242432A JP 24243288 A JP24243288 A JP 24243288A JP 2598811 B2 JP2598811 B2 JP 2598811B2
- Authority
- JP
- Japan
- Prior art keywords
- potato tuber
- tuber formation
- inducing
- potato
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【発明の詳細な説明】 （産業上の利用分野） 本発明は、馬鈴薯塊茎形成誘導剤及び同形成誘導方法
に関する。特に、組織培養方法を用いて馬鈴薯塊茎形成
誘導する際に有用な馬鈴薯塊茎形成誘導剤及び同形成誘
導方法に関する。Description: TECHNICAL FIELD The present invention relates to a potato tuber formation inducer and a method for inducing the same. In particular, the present invention relates to a potato tuber formation inducer useful for inducing potato tuber formation using a tissue culture method, and a method for inducing the same.
（従来の技術） 近年、馬鈴薯の組織培養によって得られる塊茎を馬鈴
薯の無菌的急速増殖方法に用いることが注目されてい
る。この方法においては、馬鈴薯植物を組織培養して、
塊茎を形成誘導する点にポイントがある。(Prior Art) In recent years, attention has been paid to using tubers obtained by tissue culture of potato in a method for aseptic rapid propagation of potato. In this method, a potato plant is tissue-cultured,
There is a point in inducing tuber formation.
塊茎を形成誘導するのに適する組織培養培地の組成
が、「園芸学会昭和62年秋季大会誌」第266、227頁（発
表者、秋田 求、高山真策）において、既に提案されて
いる。The composition of a tissue culture medium suitable for inducing the formation of tubers has already been proposed in “Journal of the Horticultural Society Fall Meeting 1987,” pp. 266, 227 (presenter, Noboru Akita, Makoto Takayama).
同刊行物では、まず、組織培養培地であるムラシゲ−
スクーグ（Murasige−Skoog）培地のシュークロース濃
度を３％に調整した培地で組織培養して、無菌シュート
を育成（Phase1）し、次に、同培地のシュークロース濃
度を高濃度（９％）に調整した培地で組織培養（Phase
2）して、塊茎の形成量を増大させたことが報告されて
いる。In this publication, first, Murashige, a tissue culture medium,
Tissue culture is performed on a medium in which the sucrose concentration of the sucrose (Murasige-Skoog) medium is adjusted to 3% to grow aseptic shoots (Phase 1). Then, the sucrose concentration of the medium is increased to 9%. Tissue culture (Phase
2) It was reported that tuber formation was increased.
この方法では、Phase1で育成された無菌シュートをPh
ase2の培地に移植するか、Phase2の培地に取り替えるこ
とを必要とし、この際、多大の労力を要する点に課題が
あった。In this method, aseptic shoots grown in Phase 1 are
It was necessary to transplant to an ase2 medium or replace it with a Phase2 medium, and at this time, there was a problem that a great deal of labor was required.
（発明が解決しようとする課題） 本発明は、従来技術に見られる上記課題を解決すると
ともに、一層有効な馬鈴薯塊茎形成誘導剤及び同剤を用
いた馬鈴薯塊茎形成誘導方法を提供せんとするものであ
る。(Problems to be Solved by the Invention) The present invention solves the above-mentioned problems found in the prior art and provides a more effective potato tuber formation inducer and a method for inducing potato tuber formation using the same. It is.
（課題を解決するための手段）及び（作用） 本発明は、アスコルビン酸とジャスモン酸類化合物と
を有効成分として含有することを特徴とする馬鈴薯塊茎
形成誘導剤、アスコルビン酸とジャスモン酸類化合物と
サイトカイニン酸化合物とを有効成分として含有するこ
とを特徴とする馬鈴薯塊茎形成誘導剤及び前記二剤のい
ずれかを組織培養培地中に添加することを特徴とする馬
鈴薯塊茎形成誘導方法を要旨とするものである。(Means for Solving the Problems) and (Action) The present invention provides a potato tuber formation inducer comprising ascorbic acid and a jasmonic acid compound as active ingredients, ascorbic acid, a jasmonic acid compound, and cytokinic acid. A potato tuber formation inducer characterized by containing a compound as an active ingredient and a potato tuber formation induction method characterized by adding any of the two agents to a tissue culture medium. .
本発明に用いられるジャスモン酸類化合物とは、次の
一般式Ｉ又はIIで表される化合物である。The jasmonic acid compound used in the present invention is a compound represented by the following general formula I or II.
上記ジャスモン酸類化合物は、好ましくは、12−β−
ｏ−Ｄ−グルコピラノシロキシ−ジャスモン酸、メチル
ジャスモン酸、ジャスモン酸又は６−ヒドロキシ−ジャ
スモン酸である。 The jasmonic acid compound is preferably 12-β-
o-D-glucopyranosyloxy-jasmonic acid, methyljasmonic acid, jasmonic acid or 6-hydroxy-jasmonic acid.
本発明に用いられるサイトカイニン類化合物とは、カ
イネチン、セニルアミノプリン、フェニルアミノプリ
ン、ベンジルアミノプリン、シクロヘキシルアミノプリ
ン、ｏ−クロロベンジルアミノプリン、ｏ−メチルベン
ジルアミノプリン、ジフェニル尿素、４−ピリジルフェ
ニル尿素、４−ベンジルアミノベンズイミダゾール、６
−イソペンテニルアミノプリン、トランス−ゼアチン、
シス−ゼアチン、トランスゼアチンリボシド、トランス
ゼアチンモノリボチド、ジヒドロゼアチンなどである。Cytokinins used in the present invention include kinetin, phenylaminopurine, phenylaminopurine, benzylaminopurine, cyclohexylaminopurine, o-chlorobenzylaminopurine, o-methylbenzylaminopurine, diphenylurea, 4-pyridyl Phenylurea, 4-benzylaminobenzimidazole, 6
-Isopentenylaminopurine, trans-zeatin,
Cis-zeatin, transzeatin riboside, transzeatin monoribotide, dihydrozeatin and the like.
馬齢薯塊茎を形成誘導するためには、まず、馬鈴薯植
物の茎頂点培養又は発根移法により育成した頂芽又は節
を含む茎断片（以下、これを「切片」という。）を組織
培養培地で約４週間育成して、無菌シュートを得る。次
に、無菌シュートの培地中に、アスコルビン酸100〜500
0ppm、好ましくは500〜2000ppmとジャスモン酸類化合物
0.3〜12ppm、好ましくは１〜5ppmとを添加し、さらに２
〜４週間培養すると無菌シュートの節に塊茎が形成誘導
されるのである。In order to induce the formation of potato tubers, first, a stem fragment containing apical buds or nodes (hereinafter, referred to as “slice”) grown by potato plant stem apex culture or root transfer method is used as a tissue culture medium. And grow for about 4 weeks to obtain aseptic shoots. Next, in a medium of aseptic shoots, ascorbic acid 100-500
0 ppm, preferably 500-2000 ppm and jasmonic acid compounds
0.3 to 12 ppm, preferably 1 to 5 ppm, and 2
After culturing for up to 4 weeks, tuber formation is induced in the nodes of the aseptic shoot.
同様にして、無菌シュートの培地中に、アスコルビン
酸100〜5000ppm、好ましくは500〜2000ppmとジャスモン
酸類化合物0.3〜12ppm、好ましくは１〜5ppmとサイトカ
イニン類化合物0.5〜10ppm、好ましくは１〜5ppmを添加
し、さらに２〜４週間培養すると無菌シュートの節に塊
茎が形成誘導されるのである。Similarly, in the medium of the aseptic shoot, 100-5000 ppm of ascorbic acid, preferably 500-2000 ppm and jasmonic acid compound 0.3-12 ppm, preferably 1-5 ppm and cytokinin compound 0.5-10 ppm, preferably 1-5 ppm are added. When the culture is further continued for 2 to 4 weeks, tuber formation is induced in the node of the aseptic shoot.
（実施例） 馬鈴薯切片を組織培養する培地として、第１表に示す
組成を有するリンスマイヤー−スクーグ（Linsmaier＆S
koog）培地（以下、「LS培地」と略称する。）を用い
た。(Example) As a medium for tissue culture of potato slices, Linsmaier & Skoog (Linsmaier & S) having the composition shown in Table 1 was used.
koog) medium (hereinafter abbreviated as "LS medium").
組織培養は、直径2.2cm、高さ15cmの管ビン中にLS培
地10mlを入れ、20℃連続明条件で４週間培養し、平均茎
長12cmの無菌シュートを育成した。無菌シュートを切断
して得た切片を、さらに、同様の条件で培養を繰り返
し、供試無菌シュートを必要数育成した。 For tissue culture, 10 ml of LS medium was placed in a tube bottle having a diameter of 2.2 cm and a height of 15 cm, and cultured under continuous light conditions at 20 ° C. for 4 weeks to grow aseptic shoots having an average stem length of 12 cm. The sections obtained by cutting the aseptic shoots were further cultured under the same conditions, and the required number of test aseptic shoots were grown.
このようにして得た無菌シュートの管ビン中に、予め
第２表に示す組成に調整した水溶液各100μｌを添加し
た。さらに、20℃連続暗条件に置き、２週間後及び４週
間後に塊茎の形成数を数えた。Into the vial of the thus-obtained aseptic shoot, 100 μl of each aqueous solution adjusted in advance to the composition shown in Table 2 was added. Furthermore, the tuber was placed in a continuous dark condition at 20 ° C., and the number of tubers formed was counted after 2 weeks and 4 weeks.
対照区は、Ｌ−アスコルビン酸、12−β−ｏ−Ｄ−グ
ルコピラノシロキシ−ジャスモン酸、メチルジャスモン
酸、ジャスモン酸、６−ヒドロキシ−ジャスモン酸及び
カイネチンをそれぞれ単独に、同様にして添加した単独
使用区並びにシュークロース濃度を９％に調整したLS培
地に、無菌シュートを移植した従来法区とし、組織培養
条件は、いずれも同一とした。 In the control group, L-ascorbic acid, 12-β-o-D-glucopyranosyloxy-jasmonic acid, methyljasmonic acid, jasmonic acid, 6-hydroxy-jasmonic acid, and kinetin were separately added in the same manner. A conventional method in which a sterile shoot was transplanted to a single use group and an LS medium in which the sucrose concentration was adjusted to 9% was used, and the tissue culture conditions were the same.
その結果を第３表に示す。 Table 3 shows the results.
第２表から明らかな通り、本発明区１〜５は、いずれ
も２及び４週間後の塊茎形成数で、従来法区を上回り、
優れた塊茎形成能があることを示した。単独使用区は、
いずれも塊茎をほとんど形成しなかった。 As is clear from Table 2, the present invention plots 1 to 5 all exceeded the conventional plots in the number of tubers formed after 2 and 4 weeks.
It showed excellent tuber-forming ability. Single use district,
Neither formed tubers almost.
（効果） 本発明の馬鈴薯塊茎形成誘導剤及び馬鈴薯塊茎形成誘
導方法によって、馬鈴薯植物の組織培養によって、大量
の塊茎を確実に形成誘導することができる。(Effect) By the potato tuber formation inducer and the potato tuber formation induction method of the present invention, formation of a large amount of tubers can be reliably induced by tissue culture of a potato plant.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 幸田 泰則 北海道札幌市白石区もみじ台西７丁目４ 番４号 (72)発明者 吉原 照彦 北海道札幌市豊平区西岡四条14丁目４番 43号 審査官 郡山 順 (56)参考文献 特開 昭62−55075（ＪＰ，Ａ) 特開 昭62−48376（ＪＰ，Ａ) 特開 昭60−62997（ＪＰ，Ａ) ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Yasunori Koda 7-4-4 Momijidainishi, Shiroishi-ku, Sapporo-city, Hokkaido (56) References JP-A-62-55075 (JP, A) JP-A-62-48376 (JP, A) JP-A-60-62997 (JP, A)
Claims (5)
を有効成分として含有することを特徴とする馬鈴薯塊茎
形成誘導剤。1. An agent for inducing potato tuber formation, comprising ascorbic acid and a jasmonic acid compound as active ingredients.
グルコピラノシロキシ−ジャスモン酸、メチルジャスモ
ン酸、ジャスモン酸又は６−ヒドロキシ−ジャスモン酸
である請求項１の馬鈴薯塊茎形成誘導剤。2. Jasmonic acid compound is 12-β-o-D-
The potato tuber tube formation agent according to claim 1, which is glucopyranosiloxy-jasmonic acid, methyljasmonic acid, jasmonic acid or 6-hydroxy-jasmonic acid.
て含有する請求項１又は２の馬鈴薯塊茎形成誘導剤。3. The potato tuber formation inducer according to claim 1, further comprising a cytokinic acid compound as an active ingredient.
る請求項３の馬鈴薯塊茎形成誘導剤。4. The potato tuber formation inducer according to claim 3, wherein the cytokinic acid compound is kinetin.
の馬鈴薯塊茎形成誘導剤を添加することを特徴とする馬
鈴薯塊茎形成誘導方法。5. The method according to claim 1, 2, 3 or 4 in a tissue culture medium.
A method for inducing potato tuber formation, which comprises adding a potato tuber formation inducer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63242432A JP2598811B2 (en) | 1988-09-29 | 1988-09-29 | Potato tuber formation inducer and method for inducing the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63242432A JP2598811B2 (en) | 1988-09-29 | 1988-09-29 | Potato tuber formation inducer and method for inducing the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0292220A JPH0292220A (en) | 1990-04-03 |
| JP2598811B2 true JP2598811B2 (en) | 1997-04-09 |
Family
ID=17089008
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63242432A Expired - Lifetime JP2598811B2 (en) | 1988-09-29 | 1988-09-29 | Potato tuber formation inducer and method for inducing the same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2598811B2 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5436226A (en) * | 1993-11-03 | 1995-07-25 | The United States Of America, As Represented By The Secretary Of Agriculture | Natural suppression of sprouting in stored potatoes using jasmonates |
| NL1001620C2 (en) | 1995-06-22 | 1996-12-24 | Instituut Voor Agrobiologisch | Improvement in activity of plant growth regulators |
| GB9703628D0 (en) * | 1997-02-21 | 1997-04-09 | Abdelrahman Layla Z | Sugar cane production |
| CA2415062A1 (en) * | 2002-12-23 | 2004-06-23 | Unknown | Methods and genetic sequences for modulating tuber formation in tuber-producing plants, and plants genetically modified to alter tuber size and number |
| CN102405831B (en) * | 2011-08-15 | 2012-12-05 | 山东省农业科学院蔬菜研究所 | Solid culture medium for callus seedlings of stem tips of potatoes |
-
1988
- 1988-09-29 JP JP63242432A patent/JP2598811B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0292220A (en) | 1990-04-03 |
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