JP2540566B2 - Hollow fiber membrane for blood cleaning and blood cleaning device - Google Patents

Description

TECHNICAL FIELD The present invention relates to a hollow fiber membrane for blood washing and a blood washing apparatus. More specifically, the present invention relates to a blood washing hollow fiber membrane and a blood washing apparatus for removing a protective solution added to blood to be cryopreserved.

(Prior art) In recent years, blood transfusion is a method of transfusing blood containing all the components obtained by donating blood, so-called whole blood, and is a component transfusion in which only the blood components required by the patient are fractionated from the blood of another person Have moved to. Such component transfusion has less burden on the circulatory system compared with whole blood transfusion, and avoids and reduces immunological side effects. Since only large amounts of necessary components can be transfused at one time, the percentage of whole blood is small. It is possible to expect sufficient effects even with ingredients, and it is possible to effectively use ingredients that are not necessary for one patient for other patients [Tanaka and Shimizu, Comprehensive clinical practice vol.35, N
O.11 (1986)], etc., but this component transfusion is not sufficient from the viewpoint of avoiding infection and sensitization, which are the two major side effects associated with transfusion.
Infection with AIDS, ATLA, etc., or sensitization, especially in children, has been regarded as a problem, and preventive measures against these have been earnestly desired.

Recently, as a method capable of solving such a problem, an autologous blood transfusion method of transfusing a blood sampled in advance has attracted attention. If you use your own blood, problems such as virus infection and sensitization as described above do not occur essentially, and even an extremely rare blood type person can be prepared for an emergency.

In such an autologous blood transfusion method, the collected blood is separated into its components and then stored frozen.
Currently, blood can be stored for up to 42 days if it remains a liquid, but such cryopreservation allows semi-permanent storage, thus establishing a long-term safe blood transfusion system.

(Problems to be Solved by the Invention) By the way, if blood that has been separated into its components is directly frozen and stored, each component such as red blood cells, white blood cells, and platelets will be destroyed. It is necessary to add a protective solution to. At present, this protective solution varies depending on each research institution, etc., and of course depending on the target blood component, but most of them contain glycerin, dimethyl sulfoxide, etc. When returning the stored blood to the body, it is necessary to prepare a deprotection liquid by diluting and washing the stored blood after thawing.

Examples of the dilution / washing method currently performed include, for example, red blood cell concentrated liquid, Huggins liquid (glycerin 79%, glucose 8%, fructose 1%, EDTA-2.
50% when slowly frozen by adding sodium salt 0.3%)
First wash with glucose solution + 5% fructose solution, second wash with 5% fructose solution, third wash with 5% fructose solution, fourth wash with physiological saline, then saline Re-floating to [Massachusetts General Hospital (Haggin
g), National Fukuoka Central Hospital (Sumida)] In addition, when red blood cell concentrate was added to a raw solution (glycerin 28%, mannitol 3%, sodium chloride 0.65%) and rapidly frozen, the first spin-off was performed. Discard the supernatant, the second time add 0.45% NaCl solution with 15% mannitol, spin down and discard the supernatant.
In the 4th and 4th rounds, a method of adding 0.9% NaCl solution, centrifuging, discarding the supernatant, and finally resuspending it in physiological saline is adopted [New York Blood Center (Rowe)].

As described above, currently, the stored blood is diluted / washed by the badge method, and requires centrifugation or centrifugation. The recovery efficiency of the components was also not sufficient.

Therefore, it is an object of the present invention to provide a blood washing hollow fiber membrane and a blood washing apparatus that facilitate the washing operation of frozen blood. It is another object of the present invention to provide a blood washing hollow fiber membrane and a blood washing apparatus that enable aseptic and continuous washing operations. It is another object of the present invention to provide a blood washing hollow fiber membrane and a blood washing device with high blood component recovery efficiency.

(Means for Solving Problems) The above-mentioned objects are that a hydrophilic thin layer formed by grafting a hydrophilic compound with γ-rays is formed on at least the inner surface and inner surface of pores of a hydrophobic porous hollow fiber membrane. And a hollow fiber membrane for blood washing.

In the hollow fiber membrane for blood washing of the present invention, an embodiment in which the hydrophilic compound is glycerin is preferably shown. Further, in the blood washing hollow fiber membrane of the present invention, the hydrophobic porous hollow fiber membrane is a polyolefin-based, polyester-based, polyamide-based, polyurethane-based, poly (meth) acrylate-based, poly (meth) acrylonitrile-based, polysulfone-based, polysulfone-based A preferred embodiment is one which is made of any one selected from the group consisting of vinyl chloride type and polymer blends thereof, more preferably one made of polypropylene. Further, in the blood washing hollow fiber membrane of the present invention, the inner diameter is 100 to 500 μm, and the wall thickness is 5 to 30.
A preferred embodiment is μm. In the blood washing hollow fiber membrane of the present invention, the average pore diameter is 0.01 to 6.5 μm.
In the above, an embodiment having a porosity of 30 to 75% is preferable.

The above objects are also to provide a hollow fiber membrane for blood washing in which a hydrophilic thin layer obtained by grafting a hydrophilic compound by γ-rays is formed on at least the inner surface and inner surface of pores of a hydrophobic porous hollow fiber membrane in a housing. A large number of the hollow fiber membranes, the inner space of the hollow fiber membrane is communicated with the blood inlet and the blood outlet provided in the housing, and is formed by the inner peripheral surface of the housing and the outer surface of the porous hollow fiber membrane. This can also be achieved by a blood washing device in which a washing liquid inlet and a washing liquid outlet provided in the housing are connected to the space.

In the blood washing apparatus of the present invention, a mode in which it is γ-ray sterilized is preferably shown. Further, in the blood washing apparatus of the present invention, an embodiment in which the hydrophilic compound is glycerin is preferably shown. Furthermore, in the blood washing apparatus of the present invention, the hydrophobic porous hollow fiber membrane is a polyolefin type, polyester type, polyamide type, polyurethane type, poly (meth) acrylate type, poly (meth) acrylonitrile type, polysulfone type, polyvinyl chloride type. And those composed of any one selected from the group consisting of these polymer blends, more preferably polypropylene. Further, in the blood washing device of the present invention, the inner diameter of the blood washing hollow fiber membrane is 100 to 500.
An embodiment in which the thickness is μm and the wall thickness is 5 to 30 μm is preferably shown. Furthermore, in the blood washing device of the present invention, the hollow fiber membrane for blood washing has an average pore diameter of 0.01 to 6.5 μm and a porosity of 30.
A preferred embodiment is ~ 75%.

(Function) Thus, the blood washing hollow fiber membrane of the present invention has a hydrophilic thin layer formed by γ-ray grafting of a hydrophilic compound on at least the inner surface and the inner surface of the pores of the hydrophobic porous hollow fiber membrane. It is characterized by being present.

The present inventors have used a porous hollow fiber membrane as found in hemodialysis in an artificial kidney to enable continuous treatment as a method for washing stored blood after thawing in a cryopreservation system for blood. Came up with a cleaning method. That is, a washing device was prepared in which a large number of porous hollow fiber membranes were housed in a housing, and stored blood was sent to the internal space of the large number of porous hollow fiber membranes. A cleaning liquid is caused to flow in the space formed by the outer surface and the inner peripheral surface of the housing, and the blood protection liquid component such as glycerin contained in the stored blood is permeated and removed to the cleaning liquid side through the hollow fiber membrane due to the concentration difference Is. A plurality of such washing devices are connected in series (the blood outlet of one blood washing device is connected to the blood inlet of the next blood washing device to connect the blood flow paths of the plurality of blood devices). By doing so, it becomes possible to continuously perform the cleaning process in several stages in a closed system. However, when a hydrophilic porous hollow fiber membrane such as a copper ammonia regenerated cellulose membrane conventionally used for hemodialysis is used for such washing treatment, the hydrophilic porous hollow fiber membrane shortens the priming time. Water or 5 to be filled in the blood cleaning device until use
Protective liquid such as glycerin contained in stored blood because it swells immediately with a 10% glycerin aqueous solution or when it comes into contact with stored blood or a washing liquid, which causes the pores of the porous membrane to be blocked or reduced. It was impossible to sufficiently permeate and remove the components. Further, it has been concluded that such a hydrophilic porous hollow fiber membrane is not sufficient in terms of physical properties such as mechanical strength and is not very suitable as a blood washing hollow fiber membrane.

Therefore, as a result of intensive studies, the present inventors filled the inner space of the hydrophobic porous hollow fiber membrane with a hydrophilic compound, and irradiating with γ-rays in this state to obtain at least the hydrophobic porous hollow fiber membrane. A hydrophilic thin layer is formed on the inner surface and the inner surface of the holes,
The hydrophilized porous hollow fiber membrane thus obtained has sufficient hydrophilicity and mechanical strength, and has the problem of pore clogging or reduction due to swelling as described above when used for blood washing applications. It has been found that the protective liquid components such as glycerin contained in the preserved blood can be sufficiently and efficiently removed with no problem, and with extremely good permeability characteristics. In addition, after arranging the hydrophobic porous hollow fiber membrane in the housing of the blood washing device, if the hydrophilic compound is filled in the internal space of the hydrophobic porous hollow fiber membrane to perform γ-ray treatment as described above. As described above, the present inventors have reached the conclusion that the method is extremely preferable because the hydrophobic porous hollow fiber membrane can be hydrophilized and the blood washing apparatus can be sterilized by γ-rays at the same time.

Hereinafter, the present invention will be described in more detail based on embodiments.

FIG. 1 is a drawing schematically showing the fine structure of the blood washing hollow fiber membrane of the present invention. As shown in FIG. 1, a blood washing hollow fiber membrane 1 of the present invention is a hydrophilic thin film obtained by grafting a hydrophilic compound on at least an inner surface 3 and a hole inner surface 4 of a hydrophobic porous hollow fiber membrane 2 with γ rays. It is characterized in that the layer 5 is formed.

The hydrophobic porous hollow fiber membrane 1 used as a substrate in the blood washing hollow fiber membrane of the present invention is not particularly limited, and examples thereof include polyolefin-based, polyester-based, polyamide-based, polyurethane-based, and poly (meth) acrylate-based. Various kinds of hydrophobic synthetic resins such as poly (meth) acrylonitrile-based, polysulfone-based, polyvinyl chloride-based and polymer blends thereof can be used, but especially polyolefin-based ones from the viewpoint of γ-ray resistance and mechanical strength. Is preferred, and polypropylene is most preferred.
Further, the method for producing a porous hollow fiber membrane composed of such a hydrophobic synthetic resin is not particularly limited,
It can be formed into a film by a conventionally known method, for example, a method of melt-spinning into a hollow fiber and then imparting porosity by hot drawing, a compound which is easily soluble in a solvent in a polymer dope and melt-spun and then hollow. A method of imparting porosity by contacting a substance with a solvent to extract and remove the compound,
Alternatively, in the polymer dope, a compound partially soluble in the polymer and easily soluble in the solvent is blended, melt-spun, the hollow material is brought into contact with the solvent, dried, and further stretched to increase porosity. Any method such as giving may be adopted. As a method for producing such a porous membrane, more specifically, polypropylene is melt-spun at a spinning temperature of 210 to 270 ° C. and a draft ratio of 180 to 600 using a nozzle for producing a hollow fiber, and then at 155 ° C. or less. After 1st stage heat treatment 1
A method of stretching 30 to 200% at a temperature of less than 10 ° C and then performing a second heat treatment at a temperature of the first stage heat treatment temperature or higher and 155 ° C or lower (Japanese Patent Publication No.
-52,123), a mixture of a polymer having stretchability and a compound partially compatible with the polymer and easily soluble in a solvent, and melt-spun to form a hollow fiber. ,
The molded body is treated with a solvent, dried and then uniaxially or biaxially stretched by 50 to 1500% to impart porosity (Japanese Patent Publication No. 57-20970), polyolefin, and the polyolefin under melting of the polyolefin. And an organic filler that is uniformly soluble in the extract and is easily soluble in the extract to be used and, if necessary, a crystal nucleating agent are kneaded, and the kneaded product thus obtained is discharged in a molten state from the annular spinning hole. Then, the hollow material is brought into contact with a cooling and solidifying liquid which does not dissolve the polyolefin to be cooled and solidified, and then the hollow solid which is cooled and solidified is brought into contact with an extract liquid which does not dissolve the polyolefin to extract and remove the organic filler. Examples thereof include methods (JP-A-61-90703 and JP-A-61-90705). The microstructure such as the pore diameter and the porosity of the hydrophobic porous hollow fiber membrane thus produced is determined according to the type of stored blood to be treated. For example, when the preserved blood to be treated is a concentrated red blood cell solution, the average pore diameter depends on the type of hydrophobic synthetic resin and the manufacturing method of the hollow fiber membrane.
It is desirable that the porosity is 0.01 to 6.5 μm, more preferably 1.0 to 6.0 μm, and the porosity is 30 to 75%, and more preferably 60 to 75%. That is, the average pore diameter is 0.01 μm
If it is less than this, the permeation of protective liquid components such as glycerin contained in stored blood may not be sufficient, and the blood treatment time may be prolonged, while the average pore diameter is 6.5 μm. This is because if it exceeds the range, even red blood cells may be permeated during the washing process. In addition,
If the stored blood to be processed is the platelet fraction,
The average pore diameter is 0.01 to 1.5 μm, more preferably 1.0 to 1.3
If the stored blood to be treated is a leukocyte fraction, the average pore diameter is 0.01 to 12.0.
m, more preferably about 1.0 to 10.0 m, respectively. If the porosity is less than 30%, the blood treatment efficiency will be reduced, while if the porosity is more than 75%, the mechanical strength of the hollow fiber membrane will be reduced to the extent that it cannot be practically used. This is because there is a fear. Further, in this hollow fiber membrane for blood washing, the inner diameter is 100 to 500 μm, more preferably 200 from the viewpoint of permeability, mechanical strength, or volume when assembled into a module such as a blood washing apparatus.
~ 300μm, wall thickness 5 ~ 30μm, more preferably 8 ~ 15
μm is desired.

Further, it has sufficient hydrophilicity as a hydrophilic compound grafted with γ-rays on at least the inner surface 3 and the inner surface 4 of the pores of the hydrophobic porous hollow fiber membrane 2 as described above, and desirably has high physiological safety. If it is one, it is not particularly limited,
For example, water-soluble alcohols such as methanol, ethanol, glycerin, low-molecular-weight sugars such as fructose, glucose, mannitol, alanine, arginine, cysteine and water-soluble amino acids, dicarboxylic acids such as oxalic acid, malonic acid, and succinic acid, glyoxyl Acid, pyruvic acid,
Ketoacetic acid such as acetoacetic acid, glycolic acid, lactic acid, α
-Hydroxy acids such as hydroxyacetic acid, acrylic acid,
Unsaturated carbonyl compounds such as maleic acid and methacrylic acid and their sodium or potassium salts can be used, but glycerin is particularly preferable. When glycerin is used, a concentrated glycerin solution may be used, but a glycerin aqueous solution having a concentration of 5 to 10% may be used, for example.

To form the hydrophilic thin layer 5 made of such a hydrophilic compound on the inner surface 3 and the inner surface 4 of the pores of the hydrophobic porous hollow fiber membrane 2 as described above, for example, the inside of the hydrophobic porous hollow fiber membrane 2 is formed. The space may be filled with a hydrophilic compound, and γ-rays may be applied to the hydrophobic porous hollow fiber membrane in this state. It should be noted that it is desired that the γ-ray irradiation condition is 0.1 to 25 Mrad, preferably about 2.5 to 10 Mrad. And after γ irradiation,
Excessive hydrophilic compound that has not been graft-bonded to the hydrophobic porous membrane 2 is discharged from the internal space of the hydrophobic porous membrane 2,
The desired hollow fiber membrane for blood washing 1 can be obtained by sufficiently removing the hydrophilic compound attached and remaining in the internal space of the hydrophobic porous membrane 2 by using aseptic distilled water or physiological saline. . Furthermore, when the hollow fiber membrane for blood washing of the present invention is used in a blood washing apparatus or the like, after the hydrophobic porous hollow fiber membrane 2 is assembled in the blood washing apparatus as described above, the hydrophilic compound is added in this way. It is extremely desirable to perform the operation of filling and irradiating with γ-rays, because the blood washing apparatus can be sterilized by γ-rays at the same time.

The hydrophilic thin layer 5 formed in this manner is sufficient because the hydrophilic compound as described above is grafted onto the inner surface 3 and the inner surface 4 of the pores of the hydrophobic porous hollow fiber membrane 2 by γ rays. Even though it is very thin, even if it is in a wet state, it does not substantially change the membrane structure of the hydrophobic porous membrane even though it imparts such hydrophilicity to the porous hollow fiber membrane, and thus is stable. It shows the permeation performance.

The blood washing apparatus of the present invention has a large number of such hollow fiber membranes for blood washing arranged in the housing, and makes the internal space of the hollow fiber membranes communicate with the blood inlet and the blood outlet provided in the housing. On the other hand, a space formed by the inner peripheral surface of the housing and the outer surface of the porous hollow fiber membrane is connected to a washing liquid inlet and a washing liquid outlet provided in the housing, and the stored blood is stored in the inner space of the hollow fiber membrane. On the other hand, a washing solution is circulated to the outside of the blood washing hollow fiber membrane, and a protective liquid component such as glycerin that is permeable to the blood washing hollow fiber membrane contained in stored blood is moved to the washing liquid side due to a concentration difference. To clean.

FIG. 2 is a schematic cross-sectional view showing the structure of one embodiment of the blood cleaning device of the present invention. The blood cleaning apparatus 10 of the present embodiment shown in FIG. 2 has a cylindrical housing body 11 with both ends open, and a lid 12a and a lid 12a which are fitted in the openings at both ends of the housing body 11 in a liquid-tight manner. It has a housing 13 composed of 12b, a cleaning liquid inlet 14 is provided near one end of the housing body 11, and a cleaning liquid outlet 15 is provided near the other end thereof. The lid 12a is provided with a blood inlet 16 and the lid 12b is provided with a blood outlet 17. In the inner space of the housing 13, however, a large number, for example, about 10,000, of the above-mentioned blood washing hollow fiber membranes 1 are arranged along the axial direction of the housing 13 so as to be separated from each other. Both ends of the blood-cleaning hollow fiber membrane 1 are supported by the housing body 13 by partition walls 18a and 18b made of potting agent filled in the both ends of the housing body 13 in a state where the respective openings are not closed. . Further, the partition walls 18a and 18b are attached to the housing 13
The inner space of is divided into three. That is, the housing 13
The inner space of the housing body 11 is surrounded by the inner peripheral surface of the housing body 11 and the partition wall 18a, and communicates with the blood introducing port 16 and the inner space of the blood washing hollow fiber membrane 1. Surface and outer surface of blood cleaning hollow fiber membrane 1 and partition wall
It is surrounded by 18a and 18b, and is surrounded by the cleaning liquid flow space 20 communicating with the cleaning liquid inlet 14 and the cleaning liquid outlet 15, the inner peripheral surface of the housing body 11 and the partition wall 18b, and the blood outlet 17
In addition, a blood circulation space 19b communicating with the internal space of the blood washing hollow fiber membrane 1 is formed. In this way, the partition walls 18a, 18
b plays an important function of separating the inside and the outside of the blood washing hollow fiber membrane, and normally, the partition walls 18a, 1
8b can be formed by inflowing polyurethane, silicone, epoxy resin or the like into the inner wall surfaces of both ends of the housing body 11 using a centrifugal injection method or the like and curing.

The blood cleaning device having the above-mentioned configuration is sterilized before use, and as the sterilization method, an autoclave sterilization method, a γ-ray sterilization method, or the like can be preferably used, but in particular, as described above, blood After assembling the hydrophobic porous hollow fiber membrane, which is the base material of the hollow fiber membrane for washing, into the blood washing device, the inner space of the hydrophobic porous hollow fiber membrane was filled with a hydrophilic compound and irradiated with γ-rays. It is desirable to perform a treatment for forming a hydrophilic thin layer on the inner surface and the inner surface of the pores of the hydrophobic porous hollow fiber membrane, because the whole blood washing apparatus can be sterilized with γ-rays at the same time.

(Example) Example 1 10000 polypropylene porous hollow fiber membranes having an inner diameter of 200 µm and a wall thickness of 10 µm (average pore diameter 1.0 µm, porosity 70%) were used in a blood washing apparatus 10 as shown in Fig. 2. It is placed in a cylindrical housing 13, both ends are fixed by polyurethane potting material and the porous hollow fiber membrane is assembled in the housing 13, and an effective length of 230 mm, an effective membrane area of 1.6 m ² of the blood cleaning device 10 is installed. Created. In this way, four blood cleaning devices 10 having the same structure are created, and subsequently, the blood outlet port 17 of one device is connected to the blood inlet port 16 of the next device using a polyethylene connecting tube. This created a circuit in which four blood cleaning devices 10 were connected in series. Glycerin was caused to flow from the blood inlet 16 of the first blood cleaning device 10 in this circuit, and the inner space of the porous hollow fiber membrane 2 in each blood cleaning device 10 was filled with glycerin. In this state, the whole circuit was irradiated with γ-rays at an irradiation intensity of 8 Mrad. After irradiating with γ-ray, glycerin was eliminated from the system and further thoroughly washed with physiological saline to remove the glycerin remaining on the hollow fiber membrane.

The stored blood was washed using the circuit thus treated. That is, as the stored blood to be washed first,
Equal volume of Huggins liquid (79% glycerin, 8% glucose,
Fructose 1%, EDTA-2 sodium salt 0.3%) was added -8
Prepare a concentrated red blood cell concentrate stored at 5 ° C and
Thaw at ° C. On the other hand, after priming the circuit with physiological saline, the cleaning solution shown in Table 1 is introduced into the cleaning solution flow space 20 through the cleaning solution inlet 14 of each blood cleaning device 10 of the circuit at a flow rate of 500 ml / min. Continued to discharge from outlet 15. In this state, preserved blood 40 thawed as described above
0 ml was fed into the above circuit at a flow rate of 50 ml / min from the blood inlet of the first blood washing device 10. The washed blood flowing through the circuit and flowing out from the blood outlet 17 of the fourth blood washing device 10 was collected. The time required for this cleaning treatment was 8 minutes. The recovery rate of red blood cells was calculated by measuring the number of red blood cells before and after the washing treatment, and it was 99% .Glycerin contained in the blood after the washing treatment was 0.01 ppm, which means that the blood cells were substantially completely removed. It was

(Effects of the Invention) As described above, the present invention has a hydrophilic thin layer formed by grafting a hydrophilic compound with γ-rays on at least the inner surface and inner surface of pores of a hydrophobic porous hollow fiber membrane. Since it is a hollow fiber membrane for blood washing characterized by the following, it has sufficient hydrophilicity or water permeability, and since the hydrophilicity is imparted by the extremely thin hydrophilic thin layer as described above, even when wet. Since it does not cause a problem such as a change in membrane characteristics due to swelling and is excellent in terms of mechanical strength and the like, for example, a blood cleaning device in which a large number of the blood cleaning hollow fiber membranes are assembled is used. This makes it possible to efficiently and easily wash and remove the protective liquid component such as glycerin contained in the frozen stored blood in the frozen stored blood washing operation.

The present invention also provides a hollow fiber membrane for blood washing, in which a hydrophilic thin layer obtained by grafting a hydrophilic compound by γ-rays is formed on at least the inner surface and the inner surface of pores of a hydrophobic porous hollow fiber membrane in a housing. A large number of the hollow fiber membranes are arranged so that the inner space of the hollow fiber membranes communicates with the blood inlet port and the blood outlet port provided in the housing, while the space formed by the inner peripheral surface of the housing and the outer surface of the porous hollow fiber membranes Is a blood washing device in which a washing liquid inlet and a washing liquid outlet provided in the housing are communicated with each other. Therefore, it is possible to perform the washing operation of frozen preserved blood by using the hollow fiber membrane having the above-mentioned excellent characteristics. Therefore, the cleaning efficiency and the blood component recovery rate are extremely high, and by connecting a plurality of blood cleaning devices, the frozen stored blood cleaning operation that was conventionally performed in a batch system can be performed. Continued manner it becomes possible to carry out aseptically, and thus to greatly develop cryopreservation system of blood. Furthermore, in the blood washing apparatus of the present invention, if the hydrophilic treatment of the inner surface of the porous hollow fiber membrane housed inside the housing and the inner surface of the pores with γ-rays is carried out after assembling the apparatus, γ-ray sterilization of the blood washing apparatus can be performed at the same time. It is more convenient because it can be done.

[Brief description of drawings]

FIG. 1 is a sectional view schematically showing the fine structure of the blood washing hollow fiber membrane of the present invention, and FIG. 2 is a schematic sectional view showing the structure of an embodiment of the blood washing apparatus of the present invention. . 1 ... Hollow fiber membrane for blood cleaning, 2 ... Hydrophobic porous hollow fiber membrane, 3 ... Inner surface, 4 ... Inner surface of pore, 5 ... Hydrophilic thin layer, 10 ... Blood cleaning device, 11 ... Housing body, 12a, 12b ... Lid, 13 ... Housing, 14 ... Wash solution inlet, 15 ... Wash solution outlet, 16 ... Blood inlet, 17 ... Blood outlet, 18a, 18b ... Septa , 19a, 19b …… Blood circulation space, 20 …… Washing liquid circulation space.

Claims (13)

(57) [Claims] 1. A hollow fiber membrane for blood washing, characterized in that a hydrophilic thin layer formed by grafting a hydrophilic compound by γ-rays is formed on at least the inner surface and the inner surface of pores of a hydrophobic porous hollow fiber membrane. . 2. The blood washing hollow fiber membrane according to claim 1, wherein the hydrophilic compound is glycerin. 3. A hydrophobic porous hollow fiber membrane comprising a polyolefin type, polyester type, polyamide type, polyurethane type, poly (meth) acrylate type, poly (meth) acrylonitrile type, polysulfone type, polyvinyl chloride type and these The hollow fiber membrane for blood washing according to claim 1 or 2, which is composed of any one selected from the group consisting of polymer blends. 4. The blood washing hollow fiber membrane according to claim 3, wherein the hydrophobic porous hollow fiber membrane is made of polypropylene. 5. The blood washing hollow fiber membrane according to any one of claims 1 to 4, which has an inner diameter of 100 to 500 μm and a wall thickness of 5 to 30 μm. 6. An average pore diameter of 0.01 to 6.5 μm and a porosity of 3
The hollow fiber membrane for blood washing according to claim 1 or 2, which is 0 to 75%. 7. A hollow fiber membrane for blood washing, wherein a hydrophilic thin layer formed by grafting a hydrophilic compound with γ-rays on at least the inner surface and the inner surface of pores of a hydrophobic porous hollow fiber membrane is formed in a housing. A large number of the hollow fiber membranes are arranged so that the inner space of the hollow fiber membranes communicates with the blood inlet port and the blood outlet port provided in the housing, while the space formed by the inner peripheral surface of the housing and the outer surface of the porous hollow fiber membranes. Is a blood washing device in which a washing liquid inlet and a washing liquid outlet provided in the housing are communicated with each other. 8. The blood cleaning apparatus according to claim 1, which is sterilized by γ-rays. 9. The blood washing apparatus according to claim 7, wherein the hydrophilic compound is glycerin. 10. A hydrophobic porous hollow fiber membrane is a polyolefin-based, polyester-based, polyamide-based, polyurethane-based,
The invention according to claim 7, which is composed of any one selected from the group consisting of poly (meth) acrylate-based, poly (meth) acrylonitrile-based, polysulfone-based, polyvinyl chloride-based, and polymer blends thereof.
Item 10. A blood cleaning apparatus according to any one of items 9 to 9. 11. The blood washing apparatus according to claim 10, wherein the hydrophobic porous hollow fiber membrane is made of polypropylene. 12. An inner diameter of a blood washing hollow fiber membrane is 100 to 500 μm.
m, and the wall thickness is 5 to 30 μm.
13. The blood cleaning device according to any one of 11 items. 13. A blood cleaning hollow fiber membrane having an average pore diameter of 0.01.
The blood cleaning apparatus according to any one of claims 7 to 12, which has a porosity of 30 to 75% and a porosity of 30 to 75%.