JP2022147227A - Evaluation system and evaluation method - Google Patents

Abstract

To provide an evaluation system capable of evaluating a total amount of a plurality of polyphenol components in a solution relatively easily.SOLUTION: There is provided a system for evaluating a total amount of polyphenol components in a solution which includes, a plurality of polyphenol components which indicates absorption at a prescribed wavelength and which elutes from vegetable or fruit, the system comprises: measurement means 2 for measuring absorbance in the prescribed wavelength of the solution; and calculating means 4 for, on the basis of the absorbance measured by the measurement means 2, calculating information related to the total amount of the polyphenol components in the solution. The calculating means 4 calculates information related to the total amount of the polyphenol components in the solution, on the basis of a predetermined absorbance-total amount correlation relationship which indicates a relationship between the absorbance in the prescribed wavelength in the solution and information related to the total amount of the polyphenol components in the solution, and is predetermined on the basis of an inclusion ratio of the plurality of polyphenol components in vegetable or fruit.SELECTED DRAWING: Figure 1

Description

The present invention relates to systems and methods for assessing the total amount of polyphenolic components in aqueous solutions.

In recent years, attention has been focused on functional ingredients useful for the human body. For example, polyphenols, which are water-soluble functional ingredients, are said to be effective in improving blood flow and preventing lifestyle-related diseases and aging. Such polyphenols are used in various processed products. The contents are displayed.

Conventionally, as a method for measuring polyphenols, the Folin-Ciocalteu method has been used as an official method for measuring the amount of polyphenols contained in tea beverages and the like. As a method for measuring tannin, which is one of polyphenols, the Folin-Denis method is used as an official method for measuring the amount of tannin contained in wine, distilled spirits, and the like. These techniques are often used in research as techniques for measuring the amount of polyphenols contained in vegetables and fruits.

Further, Patent Document 1 proposes a polyphenol sensor having an oxygen electrode containing an enzyme that decomposes hydrogen peroxide and an electron mediator, and containing an enzyme that decomposes hydrogen peroxide and hydrogen peroxide in a reaction vessel. According to this polyphenol sensor, polyphenols can be quantified by electrochemically using an oxygen electrode to confirm the amount of decrease in hydrogen peroxide in a mixed solution of polyphenols and hydrogen peroxide.

JP-A-2000-321234

Both the above-mentioned Folin-Ciocalteu method and Folin-Denis method are colorimetric analyzes performed by coloring the polyphenols contained in the liquid with a reagent and measuring the absorbance, and a reagent for coloring the polyphenols is indispensable. be.

In the method using a polyphenol sensor described in Patent Document 1, measurement can be performed simply by inserting an electrode into an aqueous solution to be measured, but hydrogen peroxide or the like is required as a reagent.

Here, quercetin, which is one of polyphenols, has multiple glycosides. For example, onions contain quercetin 3-β-D-glucoside and quercetin 3,4'-diglucoside. Currently, when evaluating the total amount of quercetin eluted from onions in water, the respective amounts of quercetin 3-β-D-glucoside and quercetin 3,4′-diglucoside are measured using HPLC or the above colorimetric analysis. must be evaluated separately before the total amount of quercetin is evaluated. In other words, when multiple similar polyphenol components eluted from vegetables and fruits are contained in an aqueous solution, the total amount of multiple polyphenol components cannot be evaluated without first evaluating the amount of each similar polyphenol component. .

As described above, the colorimetric analysis and the method described in Patent Document 1 require pretreatment such as coloring with reagents and addition of reagents, so it is complicated to evaluate the amount of each of the multiple polyphenol components. Processing is required, and the time required for measurement tends to be long. In addition, the method using HPLC requires expensive equipment and takes time to evaluate the amount of each of the multiple polyphenol components.

Therefore, the method of evaluating the total amount of multiple polyphenol components after evaluating the amount of each polyphenol component has problems such as the need for pretreatment such as coloring with reagents and the addition of reagents, and the time required for measurement. There are problems such as the problem of requiring expensive equipment and the increase in cost due to the need for expensive equipment. Therefore, it is desired to develop a method that can evaluate the total amount of similar polyphenol components in an aqueous solution more simply than before without separately evaluating the amount of each polyphenol component.

The present invention has been made in view of the above circumstances, and an object of the present invention is to provide an evaluation system and an evaluation method that can relatively simply evaluate the total amount of a plurality of polyphenol components in an aqueous solution.

The inventors of the present application have conducted intensive research on a system that can evaluate the total amount of polyphenol components in an aqueous solution containing multiple polyphenol components. By predetermining the relationship between the absorbance of a predetermined wavelength for an aqueous solution containing a plurality of polyphenol components eluted from and the information on the total amount of polyphenol components in the aqueous solution, information on the total amount of polyphenol components based on this relationship The present invention was completed by discovering that it can be calculated.

That is, the characteristic configuration of the evaluation system according to the present invention for achieving the above object is as follows:
A system for evaluating the total amount of polyphenol components in an aqueous solution containing a plurality of polyphenol components eluted from vegetables or fruits and exhibiting absorption at a predetermined wavelength,
measuring means for measuring the absorbance of the aqueous solution at the predetermined wavelength;
calculation means for calculating information on the total amount of the polyphenol component in the aqueous solution based on the absorbance measured by the measurement means;
and a display means for displaying information about the total amount of the polyphenol component calculated by the calculation means,
The calculation means expresses the relationship between the absorbance of the aqueous solution at the predetermined wavelength and the information on the total amount of the polyphenol components in the aqueous solution, based on the content ratio of the plurality of polyphenol components in the vegetables or fruits The point is that information on the total amount of the polyphenol component in the aqueous solution is calculated based on a predetermined absorbance-total amount correlation.

In addition, the characteristic configuration of the evaluation method according to the present invention for achieving the above object is
A method for evaluating the total amount of polyphenol components in an aqueous solution containing a plurality of polyphenol components eluted from vegetables or fruits and exhibiting absorption at a predetermined wavelength,
a measuring step of measuring the absorbance of the aqueous solution at the predetermined wavelength;
a calculating step of calculating information on the total amount of the polyphenol component in the aqueous solution based on the absorbance measured in the measuring step;
a display step of displaying information about the total amount of the polyphenol component calculated in the calculation step;
The calculating step expresses the relationship between the absorbance of the aqueous solution at the predetermined wavelength and the information on the total amount of the polyphenol components in the aqueous solution, based on the content ratio of the plurality of polyphenol components in the vegetables or fruits. The point is that information on the total amount of the polyphenol component in the aqueous solution is calculated based on a predetermined absorbance-total amount correlation.

According to the above characteristic configuration, by predetermining the absorbance-total amount correlation, the absorbance at a predetermined wavelength for an aqueous solution containing a plurality of polyphenol components that exhibit absorption in a similar wavelength range is measured, and the measured absorbance and the absorbance-total amount correlation, it is possible to obtain information on the total amount of a plurality of polyphenol components in the aqueous solution. In other words, the total amount of a plurality of polyphenol components can be relatively easily evaluated without separately evaluating the amounts of each of the plurality of polyphenol components contained in the aqueous solution.

In addition, since the total amount of multiple polyphenol components can be evaluated relatively easily, it is possible to measure the multiple polyphenol components contained in food and beverages at the site of food preparation and processing. The total amount can be easily evaluated.

A further characteristic configuration of the evaluation system according to the present invention is
The aqueous solution contains a first polyphenol component and a second polyphenol component as the plurality of polyphenol components,
The absorbance-total amount correlation is predetermined based on the content ratio of the first polyphenol component and the second polyphenol component in the vegetable or fruit.

According to the above characteristic configuration, information on the total amount of polyphenol components (the total amount of the first polyphenol component and the second polyphenol component) in the aqueous solution containing the first polyphenol component and the second polyphenol component eluted from vegetables or fruits is obtained. can be done.

A further characteristic configuration of the evaluation system according to the present invention is
The absorbance-total amount correlation is information about the absorbance at the predetermined wavelength of the first aqueous solution containing only one of the polyphenol components of the first polyphenol and the second polyphenol, and the amount of the polyphenol component in the first aqueous solution. and the absorbance at the predetermined wavelength for the second aqueous solution containing only the other polyphenol component, and the correlation with information on the amount of the polyphenol component in the second aqueous solution. .

According to the above characteristic configuration, information on the total amount of polyphenol components (the total amount of the first polyphenol component and the second polyphenol component) in the aqueous solution containing the first polyphenol component and the second polyphenol component eluted from vegetables or fruits is obtained. can be done.

A further characteristic configuration of the evaluation system according to the present invention is
The polyphenol component is a quercetin derivative.

The inventors of the present application have found that information on the total amount of quercetin derivatives in an aqueous solution can be obtained based on the absorbance at a predetermined wavelength measured for an aqueous solution containing a plurality of different quercetin derivatives and the above absorbance-total amount correlation. Confirmed by experiments. That is, according to the above characteristic configuration, the total amount of quercetin derivatives in an aqueous solution containing a plurality of different quercetin derivatives eluted from vegetables and fruits can be evaluated.

It is a figure which shows schematic structure of the evaluation system which concerns on embodiment. It is a graph showing the absorbance-total amount correlation. 1 is a graph summarizing calculated values and measured values of quercetin concentrations in aqueous solutions.

An evaluation system and an evaluation method according to an embodiment of the present invention will be described below with reference to the drawings. In this embodiment, a case where the user is cooking by heating food (onion) in water will be described as an example.

[Evaluation system]
FIG. 1 is a diagram showing a schematic configuration of an evaluation system 1 according to this embodiment. As shown in the figure, the evaluation system 1 is a system for evaluating the total amount of polyphenol components in an aqueous solution containing a plurality of polyphenol components eluted from vegetables or fruits and exhibiting absorption at a predetermined wavelength. A measuring device 2 (measuring means) for measuring absorbance at a predetermined wavelength, a calculating unit 4 (calculating means) for calculating information on the total amount of polyphenol components in the aqueous solution based on the absorbance measured by the measuring device 2, and calculating A display device 6 (display means) for displaying information on the total amount of polyphenol components calculated in the unit 4 is provided. The evaluation system 1 of this embodiment also includes a storage unit 5 that stores the absorbance-total amount correlation, which will be described later. In the present embodiment, the calculation unit 4 and the storage unit 5 are a control device realized using one or more computer devices having an information arithmetic processing function, an information input/output function, an information storage function, etc. 3. In this case, the function of the calculation unit 4 and the function of the storage unit 5 may be installed in the computer device as a program implemented by the computer device.

Examples of a plurality of polyphenol components that are eluted from vegetables or fruits and exhibit absorption at a predetermined wavelength include quercetin 3-β-D-glucoside and quercetin 3,4'-diglucoside, which are quercetin derivatives contained in onions, burdock, and eggplant. chlorogenic acid and caffeoylquinic acid contained in, etc., but in the present embodiment, quercetin 3-β-D-glucoside (first polyphenol component) and quercetin 3,4'- which are quercetin derivatives contained in onions The two polyphenolic components are diglucosides (the second polyphenolic component).

The measurement device 2 is a spectrophotometer, and in this embodiment, a quartz cell C for a spectrophotometer containing water (aqueous solution) for heating food is set, and the quartz cell C in this set The aqueous solution is irradiated with light of a predetermined wavelength, and the absorbance of the aqueous solution is measured. The predetermined wavelength is preferably a wavelength of light at which only the polyphenol component to be evaluated among the substances contained in the aqueous solution to be measured exhibits absorption. may be the wavelength of light that exhibits absorption. Specifically, the measuring device 2 of the present embodiment irradiates the aqueous solution with light of 360 nm, which quercetin 3-β-D-glucoside and quercetin 3,4′-diglucoside of quercetin absorb, and measures the absorbance.

In this embodiment, the calculator 4 is a functional unit that calculates information on the total amount of polyphenol components based on the absorbance at 360 nm measured by the measuring device 2 .

Here, the inventors of the present application obtained the following new knowledge as a result of earnest research. That is, information on the total amount of polyphenol components in an aqueous solution containing multiple polyphenol components eluted from a certain vegetable or fruit, and the relationship between the absorbance measured at the wavelength at which the multiple polyphenol components absorb the aqueous solution, By determining in advance based on the actual content ratio of a plurality of polyphenol components in vegetables or fruits, this predetermined relationship (absorbance - total amount correlation) and the absorbance at a predetermined wavelength measured for the aqueous solution to be measured. Based on this, it was found that information on the total amount of polyphenol components in the aqueous solution to be measured can be obtained.

Specifically, the absorbance-total amount correlation can be obtained as follows. First, using a sample of the first polyphenol component and a sample of the second polyphenol component, prepare a first polyphenol aqueous solution (first aqueous solution) and a second polyphenol aqueous solution (second aqueous solution) of various concentrations, For each of the aqueous solutions, the absorbance at the wavelengths at which the first and second polyphenol components absorb is measured beforehand. Then, the relationship between the measured absorbance and concentration is plotted to create a calibration curve for each of the first polyphenol component and the second polyphenol component. These two calibration curves correspond to the correlation between the absorbance of the first aqueous solution or the second aqueous solution at a predetermined wavelength and the information regarding the amount of polyphenol component in the first aqueous solution or the second aqueous solution.

Next, the content ratio of these two polyphenols actually contained in the vegetables or fruits containing the first polyphenol component and the second polyphenol component is obtained. In addition, the content ratio may be a content ratio obtained based on the measured value by measuring the amount of two polyphenol components contained in vegetables or fruits by a known method, or a content ratio of a certain vegetable or fruit. For a plurality of varieties, the content ratios may be calculated in the same manner as described above and averaged.

Next, the calibration curve of the first polyphenol and the calibration curve of the second polyphenol prepared as described above are synthesized based on the content ratio obtained as described above. The linear function expression of the synthetic calibration curve obtained in this manner is the absorbance-total amount correlation.

The absorbance-total amount correlation obtained as described above may be stored in advance in the storage unit 5 as a linear function expression of the synthetic calibration curve, or the data necessary for creating the synthetic calibration curve may be stored in the storage unit 5. , and when calculating the information on the total amount of polyphenols (concentration in this embodiment), a synthetic calibration curve may be created and used as appropriate based on these data. In the form, it is assumed that the absorbance-total amount correlation is stored in the storage unit 5 in advance.

In this embodiment, the first polyphenol is quercetin 3-β-D-glucoside, the second polyphenol is quercetin 3,4'-diglucoside, the predetermined wavelength is 360 nm, and the average content ratio of each onion of different varieties is used. The absorbance-total correlation obtained is used.

Therefore, the calculation unit 4 of the present embodiment acquires the absorbance at 360 nm measured by the measuring device 2, and based on the absorbance-total amount correlation, quercetin 3-β-D-glucoside (first polyphenol component) and quercetin 3,4′-diglucoside (second polyphenol component) are calculated as information on the total amount of polyphenol components.

The storage unit 5 is a functional unit that stores various information. Specifically, in this embodiment, in addition to the absorbance-total amount correlation, the absorbance at a predetermined wavelength measured by the measuring device 2 can be stored. In addition, the absorbance-total amount correlation is determined based on the relationship between the amount of each of the plurality of polyphenol components and the absorbance and the content ratio of the plurality of polyphenol components in vegetables and fruits, as described above. It varies depending on the type of polyphenols and the ingredients (that is, the content ratio) that contain these polyphenol components. Therefore, the storage unit 5 stores a plurality of predetermined absorbance-total amount correlations for cases where the type of polyphenol component and the content ratio of the polyphenol component are different, and the calculation unit 4 appropriately calculates the necessary absorbance-total amount Configured to reference correlations.

The display device 6 is a device for displaying information about the total amount of polyphenol components calculated by the calculation unit 4 as described above. Concentration of quercetin combined with β-D-glucoside (first polyphenol component) and quercetin 3,4′-diglucoside (second polyphenol component) is displayed.

Next, a method for evaluating the total amount of quercetin contained in water (aqueous solution) after heating onions in water for a predetermined time in the evaluation system 1 having the above configuration will be described.

First, put the onions into the water and start heating. The onions to be added may be cut into appropriate sizes, or may be left as they are without being cut.

After heating for a predetermined time, part of the water containing the onion is put into the quartz cell C, and the absorbance at 360 nm is measured by the measuring device 2 (measurement step).

Next, the absorbance measured by the measuring device 2 is sent to the calculating unit 4 of the control device 3, and the calculating unit 4 calculates the concentration of quercetin contained in water from the measured absorbance and the absorbance-total amount correlation. (calculation step).

Thereafter, the quercetin concentration calculated by the calculator 4 is transmitted to the display device 6, and the quercetin concentration is displayed on the screen of the display device 6 (display step). Therefore, the total amount of quercetin contained in the aqueous solution can be evaluated as the concentration.

By checking the concentration of quercetin displayed on the screen of the display device 6, the user can recognize the total amount of quercetin eluted from the onions into the water at the present time.

Thus, according to the evaluation system and evaluation method according to the present embodiment, by utilizing the absorbance-total amount correlation, even if the aqueous solution contains a plurality of polyphenols that exhibit absorption at a predetermined wavelength. However, information on the total amount of a plurality of polyphenol components in the aqueous solution can be relatively easily evaluated without separately obtaining the amounts of the plurality of polyphenol components.

[Another embodiment]
[1] In the above embodiment, two types of polyphenol components, the first polyphenol component and the second polyphenol component, are contained in vegetables and fruits as a plurality of polyphenol components that exhibit absorption at a predetermined wavelength, and these are eluted in water. , but not limited to these. Vegetables and fruits may contain three or more types of polyphenol components that exhibit absorption at a predetermined wavelength, and these components may be eluted into water.

[2] In the above embodiment, the absorbance-total amount correlation is based on the two correlations regarding the first aqueous solution and the second aqueous solution, and the content ratio of the first polyphenol component and the second polyphenol component in the vegetable or fruit. is predetermined, but it is not limited to this. Vegetables or fruits contain 3 or more types of polyphenol components that show absorption at a predetermined wavelength, and if these are eluted in water, 3 or more for an aqueous solution prepared using a sample of each polyphenol component and the content ratio of three or more polyphenol components, the absorbance-total amount correlation is determined in advance.

[3] In the above embodiment, the concentration of quercetin is displayed on the display device 6, but the present invention is not limited to this. For example, by inputting the amount of heated water to the display device 6, the total mass of quercetin contained in the water is calculated based on the concentration of quercetin, and the total mass of quercetin is displayed on the display device 6. It may be in a displayed mode.

The configurations disclosed in the above embodiments (including other embodiments) can be applied in combination with configurations disclosed in other embodiments unless there is a contradiction. The described embodiment is an example, and the embodiment of the present invention is not limited to this, and can be modified as appropriate without departing from the object of the present invention.

The present invention will now be described in more detail based on examples. In addition, it cannot be overemphasized that this invention is not limited to an Example.

[Derivation of absorbance-total amount correlation]
First, using quercetin 3-β-D-glucoside and quercetin 3,4′-diglucoside manufactured by Sigma-Aldrich, quercetin 3-β-D-glucoside aqueous solutions of 10, 20, 30, 40 and 50 μg/mL and A quercetin 3,4′-diglucoside aqueous solution was prepared. Next, the absorbance at a wavelength of 360 nm was measured for these aqueous solutions, and the relationship between absorbance and concentration was plotted to prepare a calibration curve for quercetin 3-β-D-glucoside and quercetin 3,4′-diglucoside. (See Figure 2).

Next, quercetin 3-β-D-glucoside and quercetin 3,4'-diglucoside contained in five different varieties of onions (variety A to variety E) were analyzed by high performance liquid chromatography (HPLC). The ratio (% by mass) was measured, and the average of these was taken as the content ratio of quercetin 3-β-D-glucoside and quercetin 3,4′-diglucoside in onion (see Table 1).

Next, by synthesizing the calibration curve of quercetin 3-β-D-glucoside and the calibration curve of quercetin 3,4'-diglucoside prepared above based on the above content ratio, the absorbance-total correlation is obtained by synthesizing the synthetic calibration curve. was derived as a linear function of

[Concentration measurement of quercetin derivative]
Two types of onions of different varieties (variety A and variety B) were cut into 2 cm square test pieces, and about 10 g of the test pieces of each variety were placed in 30 g of separate water and heated. After 5 minutes from the start of heating, the broth was recovered.

Next, the recovered decoction is placed in a quartz cell, the absorbance at 360 nm is measured with a measuring device, and the concentration of the quercetin derivative in the decoction as a whole is calculated based on the measured absorbance and the above-derived absorbance-total amount correlation. did. 10 samples (samples A1 to A10 and samples B1 to B10) were prepared for each of the varieties A and B, and the concentration of each sample was calculated.

For comparison, the concentrations of samples A1 to A10 and samples B1 to B10 were measured by HPLC.

FIG. 3 is a graph summarizing calculated values and measured values. As can be seen from the figure, the calculated values are in good agreement with the measured values, with an average error of 5.26%.

From this, by using the absorbance-total amount correlation derived as described above, information on the total amount of the polyphenol component in an aqueous solution containing a plurality of polyphenol components that show absorption at a predetermined wavelength eluted from vegetables or fruits ( In the example, it was confirmed that the concentration) could be calculated.

The evaluation system and evaluation method of the present invention are used to evaluate the total amount of multiple polyphenol components in an aqueous solution.

1: Evaluation system 2: Measuring device (measuring means)
4: Calculation unit (calculation means)
6: display device (display means)

Claims (5)

A system for evaluating the total amount of polyphenol components in an aqueous solution containing a plurality of polyphenol components eluted from vegetables or fruits and exhibiting absorption at a predetermined wavelength,
measuring means for measuring the absorbance of the aqueous solution at the predetermined wavelength;
calculation means for calculating information on the total amount of the polyphenol component in the aqueous solution based on the absorbance measured by the measurement means;
and a display means for displaying information about the total amount of the polyphenol component calculated by the calculation means,
The calculation means expresses the relationship between the absorbance of the aqueous solution at the predetermined wavelength and the information on the total amount of the polyphenol components in the aqueous solution, based on the content ratio of the plurality of polyphenol components in the vegetables or fruits An evaluation system for calculating information on the total amount of the polyphenol component in the aqueous solution based on a predetermined absorbance-total amount correlation. The aqueous solution contains a first polyphenol component and a second polyphenol component as the plurality of polyphenol components,
2. The evaluation system according to claim 1, wherein the absorbance-total amount correlation is predetermined based on the content ratio of the first polyphenol component and the second polyphenol component in the vegetable or fruit. The absorbance-total amount correlation is information about the absorbance at the predetermined wavelength of the first aqueous solution containing only one of the polyphenol components of the first polyphenol and the second polyphenol, and the amount of the polyphenol component in the first aqueous solution. and the correlation between the absorbance at the predetermined wavelength for the second aqueous solution containing only the other polyphenol component and the information on the amount of the polyphenol component in the second aqueous solution. The rating system described in . The evaluation system according to any one of claims 1 to 3, wherein the polyphenol component is a quercetin derivative. A method for evaluating the total amount of polyphenol components in an aqueous solution containing a plurality of polyphenol components eluted from vegetables or fruits and exhibiting absorption at a predetermined wavelength,
a measuring step of measuring the absorbance of the aqueous solution at the predetermined wavelength;
a calculating step of calculating information on the total amount of the polyphenol component in the aqueous solution based on the absorbance measured in the measuring step;
a display step of displaying information about the total amount of the polyphenol component calculated in the calculation step;
The calculating step expresses the relationship between the absorbance of the aqueous solution at the predetermined wavelength and the information on the total amount of the polyphenol components in the aqueous solution, based on the content ratio of the plurality of polyphenol components in the vegetables or fruits. An evaluation method for calculating information on the total amount of the polyphenol component in the aqueous solution based on a predetermined absorbance-total amount correlation.

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