JP2022013759A - PCR test preprocessing automation device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an apparatus for automatically pretreating a container from which saliva is collected for a diagnostic test before a PCR test.
SOLUTION: A transfer robot 22 capable of moving in a three-dimensional direction, a transfer syringe 23, a saliva collection container 24, and a PCR inspection container 25 in which a mucilage dissolving agent for pretreatment is charged in advance. The transfer syringe 23 is used to grab the transfer syringe 23 and move it to the saliva collection container 24. The transfer syringe 23 sucks saliva from the saliva collection container 24, and the transfer syringe 23 that has sucked the saliva is transferred to the transfer robot. 22 is used to move to the PCR test container 25, and saliva is poured from the transfer syringe 23 into the PCR test container 25 to perform PCR test pretreatment.
[Selection diagram] FIG. 5

Description

The present invention relates to an apparatus that automatically pretreats a container from which saliva is collected for a diagnostic test before a PCR test.

As a prior art of a saliva collection container, Patent Document 1 describes a biological sample collecting device 130 shown in FIGS. 14 and 15. The collection device 130 includes a cylindrical sample container 131, one end 132 of the sample container 131 is closed, and the other end is formed with an opening 133 so that the sample can be collected inside. Has been done. The funnel 134 includes a tapered receiving portion 135 for collecting a sample from the subject, and the tube 137 having a passage 136 penetrating the receiving portion 135 can be fitted into the opening 133 of the sample container 131. The funnel 134 can be closed with a cover 138. Further, after collecting the sample in the sample container 131, it is provided with a base 139 for receiving the closed end 132 of the sample container 131 and supporting the container in a vertical position.

Further, Patent Document 2 describes the saliva pretreatment tool shown in FIGS. 16, 17, 18, 19, and 20. Using a cotton rod 142 having a soft synthetic resin sponge 141 at the tip of the grip rod 140 shown in FIG. 16, and an admixture container 147 having an opening 143, an introduction portion 144, a constricted portion 145 and a bag-shaped portion 146 shown in FIG. After inserting the cotton rod 142 in which saliva was absorbed into the soft synthetic resin sponge 141 into the mixing container 147, the soft synthetic resin sponge 141 was inserted into the constricted portion 145 of the mixing container 147 as shown in FIG. By pulling out the cotton rod 142 from the mixing container 147 while pressing the constricted portion 145 in the state, the saliva absorbed by the soft synthetic resin sponge 141 is squeezed into the bag-shaped portion 146, and further, as shown in FIG. 19, the mixing container is used. After dropping either the sodium hydroxide aqueous solution A or the acidic aqueous solution B into the 147, as shown in FIG. 20, the operation of applying finger pressure to the bag-shaped portion 146 to crush the soft synthetic resin sponge 141 is repeated. The saliva and the dropped sodium hydroxide aqueous solution A or acidic aqueous solution B may be agitated by discharge and inhalation to the soft synthetic resin sponge 141 to mix the saliva with the dropped sodium hydroxide aqueous solution A or acidic aqueous solution B. Are listed.

Further, Patent Document 3 describes the device for sample collection shown in FIGS. 21, 22 and 23. In FIG. 21, the sample collection device 150 has a lid 151 and a tube 152, and as shown in FIG. 22, which is a cross-sectional view taken along the line 1A-1A of FIG. 21, the lid 151 is an inner wall defined by an outer wall 153 and an inner wall 154. It has a chamber 155. The inner wall 154 has an internal space 156 and a blocking member 158, and the blocking member 158 can be fitted to the opening 157. The pipe 152 has a container 159, and the connecting member 161 firmly fixed to the inner surface of the pipe 152 by the cross member 160 shown in FIG. 23, which is a cross-sectional view taken along the line 1B-1B of FIG. 22, and the blocking member 158 can be connected to each other. It is possible. The fluid stored in the internal space 156 of the lid 151 can be discharged into the container 159 of the pipe 152 by separating the blocking member 158 from the opening 157.

However, the collecting device described in Patent Document 1 mainly includes a funnel 134 and a sample container 131, but when saliva is collected as a sample, the sample container 131 has a vertically long shape, so that the sample container 131 is viscous. High saliva may adhere to the inner wall surface of the sample container 131, making it difficult to collect the collected saliva. In addition, it is difficult to confirm the amount of saliva collected in the vertically elongated sample container 131.

Further, the saliva pretreatment tool described in Patent Document 2 requires a complicated operation of acupressure on the constricted portion 145 in order to squeeze the saliva into the bag-shaped portion 146.

Further, the device described in Patent Document 3 has a complicated structure and is complicated to operate.

As described above, the conventional saliva collection container has a drawback that the operation is complicated and it is difficult to confirm the amount of saliva collected. Therefore, the applicant has previously filed for a saliva collection container that is easy to operate and can easily confirm the amount of saliva collected. However, no proposal has been made for a device that automatically pretreats a container from which saliva is collected for a diagnostic test before a PCR test.

Japanese Unexamined Patent Publication No. 61-54423 Japanese Patent Application Laid-Open No. 2003-4605 Japanese Unexamined Patent Publication No. 2020-12849

The present invention is an improvement thereof in view of the above-mentioned problems of the prior art, and an object of the present invention is to provide an apparatus for automatically pretreating a container from which saliva is collected for a diagnostic test before a PCR test. Is to be.

In order to solve the above problems, the PCR test pretreatment automation device of the first invention of the present application includes a transfer robot capable of moving in a three-dimensional direction, a transfer syringe, a saliva collection container, and mucilage dissolution for pretreatment. It is equipped with a PCR test container in which the agent is pre-loaded, and the transfer syringe is used to grab the transfer syringe and move it to the saliva collection container. The transfer syringe sucks saliva from the saliva collection container and sucks saliva. The transfer syringe is moved to the PCR test container using a transfer robot, and saliva is poured from the transfer syringe into the PCR test container to perform PCR test pretreatment.

The PCR test pretreatment automation device of the second invention of the present application includes a transfer robot capable of moving in a three-dimensional direction, a transfer syringe, a saliva collection container, a PCR test container, and a mucolytic agent for pretreatment. Equipped with a dissolving agent charging device to be charged, a transfer robot is used to grab the transfer syringe and move it to the saliva collection container, suck saliva from the saliva collection container with the transfer syringe, and transfer the transfer syringe that has sucked saliva. Using a robot, the solubilizer charging device moves to the PCR test container in which the mucilage solubilizer for pretreatment is charged in advance, and saliva is charged from the transfer syringe into the PCR test container to perform PCR test pretreatment. Characterized by doing.

In the first or second invention of the present application, the PCR test pretreatment automation device of the third invention of the present application is the first barcode for associating with the subject information affixed to the saliva collection container and the first barcode information. A barcode reader, a second barcode for identifying the container attached to the PCR inspection container, a second barcode reader for reading the second barcode information, and a first barcode information and a second barcode. It is characterized by comprising an information management device having a function of associating information and a function of associating a PCR test result with subject information.

According to the first invention of the present application and the second invention of the present application, the pretreatment of the PCR test can be automatically performed. According to the third invention of the present application, it is possible to provide an accurate PCR test result without any discrepancy between the subject and the PCR test result.

FIG. 1 (a) is a cross-sectional view of an embodiment of a lid of a saliva collection container pretreated by the PCR test pretreatment automation device of the present invention, and FIG. 1 (b) is a PCR test pretreatment automation device of the present invention. It is sectional drawing of one Embodiment of the container part of the saliva collection container to be pretreated. FIG. 2 is a cross-sectional view showing a state in which one embodiment of a container portion and a lid portion of a saliva collection container pretreated by the PCR test pretreatment automation device of the present invention is stacked vertically. FIG. 3A is a perspective view of an embodiment of a container portion of a saliva collection container pretreated by the PCR test pretreatment automation device of the present invention, and FIG. 3B is the PCR test pretreatment automation device of the present invention. It is a perspective view of one Embodiment of the lid part of the saliva collection container which is pretreated. FIG. 4A is a perspective view of one embodiment of the mouthpiece, and FIG. 4B is a perspective view of another embodiment of the mouthpiece. FIG. 5 is a schematic perspective view of an embodiment of the PCR test pretreatment automation device of the present invention. FIG. 6 is a perspective view showing a first step (start of work) in the PCR test pretreatment automation device of the present invention. FIG. 7 is a perspective view showing a second step (attachment of a transfer syringe) in the PCR test pretreatment automation device of the present invention. FIG. 8 is a perspective view showing a third step (movement to a sample collection container) in the PCR test pretreatment automation device of the present invention. FIG. 9 is a perspective view showing a fourth step (suction of a sample) in the PCR test pretreatment automation device of the present invention. FIG. 10 is a perspective view showing a fifth step (movement to a test container) in the PCR test pretreatment automation device of the present invention. FIG. 11 is a perspective view showing a sixth step (loading of a sample into a test container) in the PCR test pretreatment automation device of the present invention. FIG. 12 is a diagram showing an outline of an operation sequence of the PCR test preprocessing automation device of the present invention. FIG. 13 is a diagram showing the concept of an example of an information management system in a PCR test. FIG. 14 is an exploded perspective view of the biological sample collecting device described in Patent Document 1. FIG. 15 is a cross-sectional view showing a state in which the biological sample collecting device described in Patent Document 1 is assembled. FIG. 16 is a perspective view showing a cotton swab of the saliva pretreatment tool described in Patent Document 2. FIG. 17 is a perspective view showing a mixing container of the saliva pretreatment tool described in Patent Document 2. FIG. 18 is a diagram showing a state in which saliva absorbed by a soft synthetic resin sponge is squeezed out in the saliva pretreatment tool described in Patent Document 2. FIG. 19 is a diagram showing a state in which an alkaline aqueous solution or an acidic aqueous solution is dropped into a mixing container in the saliva pretreatment tool described in Patent Document 2. FIG. 20 is a diagram showing a state in which saliva and the dropped liquid are mixed by applying acupressure to the bag-shaped portion in the saliva pretreatment tool described in Patent Document 2. FIG. 21 is an exploded side view of the sample collection device described in Patent Document 3. FIG. 22 is a cross-sectional view taken along the line 1A-1A of FIG. FIG. 23 is a cross-sectional view taken along the line 1B-1B of FIG.

Hereinafter, embodiments of the present invention will be described with reference to the accompanying drawings, but various modifications and modifications can be made without departing from the technical scope of the present invention.

The present invention relates to a device that automatically performs pretreatment of a saliva collection container from which saliva is collected for PCR test before PCR test, and PCR test refers to Polymerase Chain Reaction, which is a gene. A specific region is amplified with a "pair of primers" and a "heat-resistant DNA polymerase", and if even a small amount of the pathogen gene is present, the pathogen can be identified by amplifying it. The PCR test is a sample. It is performed by the procedure of pretreatment, RNA extraction and PCR test.

The present invention relates to an apparatus that automatically performs pretreatment of a container for collecting a sample (saliva) to be subjected to sample pretreatment before a PCR test. FIG. 1 (a) is a cross-sectional view of an embodiment of a lid of a saliva collection container pretreated by the PCR test pretreatment automation device of the present invention, and FIG. 1 (b) is a PCR test pretreatment automation device of the present invention. It is sectional drawing of one Embodiment of the container part of the saliva collection container to be pretreated. As shown in FIG. 1 (b), the saliva collection container 1 includes a container portion 4 having a closed bottom portion 2 and an opening top portion 3. The lower part of the opening top 3 reaches the closed bottom 2 via the first inclined portion 5 which is gently inclined like a funnel, and then the second inclined portion 6 which is steeply inclined, and is between the second inclined portion 6 and the closed bottom portion 2. Is formed with a first recess 7 having a substantially U-shaped cross section.

As shown in FIG. 1 (a), the central portion of the lid portion 8 that can be engaged with the opening top portion 3 of the container portion 4 is a substantially U-shaped cross section that faces downward and has a smaller curvature than the first recess portion 7. Two recessed portions 9 are formed. The first recessed portion 7 of the container portion 1 can be inserted into the second recessed portion 9 of the lid portion 8.

As shown in FIG. 1 (b), the upper end of the saliva collection container 1 is the opening top 3, and the top is open, so that the subject can easily inject saliva. When saliva is poured from the opening top 3, it is assumed that the highly viscous saliva passes through the gently inclined first inclined portion 5 and reaches the steeply inclined second inclined portion 6. In this way, since the inclination angle changes from the first inclined portion 5 to the second inclined portion 6, it is easy to visually confirm the amount of saliva input.

Since the first inclined portion 5 functions to introduce saliva introduced from the opening top 3 into the second inclined portion 6, it may be gently inclined. However, if the inclination angle of the first inclined portion 5 is too small, it takes time to introduce saliva into the second inclined portion 6. On the other hand, if the inclination angle of the first inclined portion 5 is large and the difference from the inclination angle of the second inclined portion 6 becomes small, it becomes difficult to confirm the amount of saliva input. Therefore, the inclination angle θ of the first inclined portion 5 with respect to the horizontal direction is preferably 10 to 40 °, more preferably 20 to 30 °.

The second inclined portion 6 is for collecting saliva in the first recess 7 having a substantially U-shaped cross section, and preferably has a function of facilitating the collection of saliva collected in the first recess 7. Therefore, the inclination angle of the inner surface 6a of the second inclined portion 6 with respect to the horizontal direction is preferably 60 to 90 °, more preferably 70 to 90 °, still more preferably 80 to 90 °. This is because the steeper the inclination angle of the inner surface 6a of the second inclined portion 6 with respect to the horizontal direction, the easier it is to collect saliva collected in the first recessed portion 7 having a substantially U-shaped cross section with an instrument such as a pipette. However, if the inclination angle of the inner surface 6a of the second inclined portion 6 with respect to the horizontal direction exceeds 90 °, it becomes difficult to collect saliva. Therefore, it is preferable that the inclination angle of the inner surface 6a of the second inclined portion 6 with respect to the horizontal direction is 90 ° or less.

The volume of the first recess 7 having a substantially U-shaped cross section may be any volume as long as it can collect the amount of saliva required for the PCR test, and may be, for example, a volume of about 100 to 300 mm ³ . If the volume of the first recess 7 becomes larger than necessary, even if the amount of saliva required for the PCR test is recovered from the first recess 7, a considerable amount of saliva remains in the first recess 7, so that various germs are present. Has the inconvenience of being easy to breed. Therefore, the volume of the first recess 7 is preferably a volume that can collect the minimum amount of saliva required for the PCR test.

It is preferable that the inner diameter D of the lid portion 8 shown in FIG. 1 (a) is the same as or slightly larger than the outer diameter d of the opening top portion 3 of the container portion 4 shown in FIG. 1 (b). This is to make it easy for the lid portion 8 to be firmly engaged (easily fitted) with the container portion 4.

The lid portion 8 can be engaged with the opening top portion 3 of the container portion 4, and the first recessed portion 7 of the container portion 4 can be inserted into the second recessed portion 9 of the lid portion 8, as shown in FIG. In addition, the lid portion 8 is engaged with the opening top portion 3 of the container portion 4, and the first recessed portion 7a of another container portion 4a is made into the second recessed portion 9 of the lid portion 8 (see FIG. 1A). It is possible to stack a large number of container portions and lid portions up and down by repeating the work of inserting and further engaging another lid portion 8a with the opening top portion 3a of another container portion 4a. Therefore, the efficiency of transporting the saliva collection container from the saliva collection site to the inspection site is increased. Moreover, since the second recess 9 (see FIG. 1A) has a smaller curvature than the first recess 7a (the radius of curvature of the tip of the first recess 7a is the radius of curvature of the tip of the second recess 9). (Because it is smaller), when the first recess 7a is inserted into the second recess 9, a gap is likely to be formed between the outer tip of the first recess 7a and the inner bottom surface of the second recess 9. Therefore, the container portion 4a and the lid portion 8 can be easily separated from each other.

FIG. 3A is a perspective view of an embodiment of the container portion 4 of the saliva collection container preprocessed by the PCR test pretreatment automation device of the present invention, and FIG. 3B is the PCR test pretreatment of the present invention. FIG. 4A is a perspective view of an embodiment of a lid 8 of a saliva collection container preprocessed by an automation device, FIG. 4A is a perspective view of an embodiment of a mouthpiece, and FIG. 4B is a separate mouthpiece. It is a perspective view of the embodiment. The materials of the container portion 4 and the lid portion 8 are not limited, and for example, polypropylene, silicone resin, or the like can be used.

Further, it is preferable that the container portion 4 and the lid portion 8 are transparent. This is because the amount of saliva collected in the container portion 4 can be confirmed from the outside. Further, it is preferable that the opening top portion 3 of the container portion 4 is soft. This is because it is easy for the subject to put saliva in the mouth. In that respect, the member 11 that directly touches the mouth of the mouthpiece 10 shown in FIG. 4A has an opening 12 and is preferable because saliva can be easily put in through the opening 12. Further, the member 16 that directly touches the mouth of the mouthpiece 15 shown in FIG. 4B has an opening 17, which is preferable because saliva can be easily put in through the opening 17. However, since the members 11 and 16 that come into direct contact with the mouth are held in the mouth, examples of the materials of the members 11 and 16 that come into direct contact with the mouth include EVA (ethylene vinyl acetate) resin, polyurethane, polyethylene, polyethylene terephthalate, and polypropylene. , Silicone resin, paper, etc. can be used.

As described above, the saliva collection container 1 pretreated by the PCR test pretreatment automation device includes the container portion 4 having the closed bottom portion 2 and the opening top portion 3, and the lower portion of the opening top portion 3 is gently inclined like a funnel. Following the first inclined portion 5, the closed bottom portion 2 is reached through the second inclined portion 6 having a steep inclination, and the first recess portion 7 having a substantially U-shaped cross section is provided between the second inclined portion 6 and the closed bottom portion 2. Is formed. According to the saliva collection container having such characteristics, saliva can be directly injected from the opening top portion 3, and the second inclined portion 6 having a steep slope follows the first inclined portion 5 having a gentle inclination. Since the inclination angle of the saliva path is changing, it is easy to check the amount of saliva input, and since saliva collects in the first recess 7 of the substantially U-shaped cross section, it is easy to collect saliva with an instrument such as a pipette. There is an effect.

Further, as described above, the saliva collection container pretreated by the PCR test pretreatment automation device includes a lid portion 8a that can be engaged with the opening top portion 3a of the container portion, and is below the central portion of the lid portion 8a. A second recess 9a having a substantially U-shaped cross section having a smaller curvature than the first recess 7a is formed, and the first recess 7a of the container portion can be inserted into the second recess 9a of the lid portion. It is characterized by. According to the saliva collection container having such a feature, the lid portion 8a is engaged with the opening top portion 3a of the container portion, and the first recessed portion 7a of another container portion is inserted into the second recessed portion 9a of the lid portion. Further, by repeating the work of engaging the other lid 8a with the opening top 3a of the other container, a large number of containers and the lid can be stacked up and down, so that saliva Since the efficiency of transporting the saliva collection container from the collection site to the inspection site is high and the curvature of the second recess 9a is smaller than that of the first recess 7a, the first recess 7a is inserted into the second recess 9a. Sometimes a gap is likely to be created between the two, which has the effect of making it easy to separate the container and lid.

The mouthpiece 10 shown in FIG. 4A has a member 11 that directly touches the mouth, a cylindrical member 14, and a member 13 that connects the member 11 and the member 14, but the member 13 and the member 14 are directly inserted into the mouth. Since it is not, it may be made of the same material as the member 11 that directly touches the mouth, but it does not necessarily have to be the same material. Further, the mouthpiece 16 shown in FIG. 4B has a member 16 that directly touches the mouth and a cylindrical member 18, but since the member 18 cannot be directly put into the mouth, it is made of the same material as the member 16 that directly touches the mouth. It may be, but it does not necessarily have to be the same material. However, it is said that vinyl chloride and bisphenol A may have an adverse effect on the human body, so it is preferable not to use these materials.

FIG. 5 is a schematic perspective view of an embodiment of the PCR test pretreatment automation device of the present invention, and the PCR test is performed by the device shown in FIG. 5 using the saliva collection container shown in FIGS. 1 (a) and 1 (b). Preprocessing can be done automatically. In FIG. 5, the PCR test pretreatment automation device 21 includes a transfer robot 22, a disposable transfer syringe 23, and a sample (saline) collection container including a container portion and a lid portion shown in FIGS. 1 (a) and 1 (b). It has 24 and a container for PCR inspection 25. The transfer robot 22 can move in the vertical direction and can move in the horizontal direction of the support member 27 along the pair of rails 26a and 26b. Further, the support member 27 provided with the pair of rails 26a and 26b can be moved in the vertical direction along the pair of columnar members 28a and 28b to adjust the overall height. Further, the pair of columnar members 28a and 28b can move along the rails 29a and 29b, respectively. In this way, the transfer robot 22 can move up and down, left and right, and back and forth in three-dimensional directions.

The transfer syringe 23, the sample (saliva) collection container 24, and the PCR inspection container 25 are on the conveyor 30, the conveyor 31, and the conveyor 32, respectively, on the front side in the front-rear direction (right when FIG. 5 is viewed in the horizontally long direction). It is loaded from the bottom) and transferred to the back side in the front-rear direction (upper left direction when FIG. 5 is viewed in the horizontally long direction).

A barcode 33 for associating with subject information is affixed to the side surface of the sample (saliva) collection container 24. The barcode reader 34 reads the barcode 33 while rotating the sample (saliva) collection container 24 around the vertical axis. Further, the vibrating device 35 vibrates the sample (saliva) collecting container 24 to promote the saliva to fall into the first recess 7 (see FIG. 1 (b)). Further, above the PCR inspection container 25, a camera 37 for observing the progress of the pretreatment is provided.

Next, the step of automating the pretreatment of the PCR test will be described with reference to FIGS. 6 to 11. FIG. 6 is a diagram showing the start of work. First, a sample (saliva) collection container is used with a lid attachment / detachment device (not shown) and a transfer robot 22 equipped in the PCR test pretreatment automation device 21 shown in FIG. The operation of removing the lid portion of 24 is performed. Next, as shown in FIG. 7, the transfer robot 22 is used to grab one transfer syringe 23, move it to the sample (saliva) collection container 24 as shown in FIG. 8, and as shown in FIG. , Saliva is sucked from the container portion of the sample (saliva) collection container 24 to raise the transfer syringe 23. With the transfer syringe 23 raised, the liquid leak detector 36 confirms the presence or absence of liquid leakage from the tip of the transfer syringe 23. After confirming that there is no liquid leakage, move to the PCR test container 25 as shown in FIG. A predetermined amount of the mucous lysing agent for pretreatment is previously charged into the PCR test container 25 by a solubilizer charging device (not shown). On top of that, as shown in FIG. 11, the sample (saliva) is put into the PCR test container 25 from the transfer syringe 23. When the mucolytic agent and the sample (saliva) are added, the pretreatment proceeds in the PCR test container 25. By observing the PCR inspection container 25 with the camera 37 shown in FIG. 5, the end of the pretreatment is determined. A cap (not shown) is attached to the PCR inspection container 25 determined to be completed in the pretreatment by a cap desorbing device (not shown). As described above, the PCR test container 25 containing the sample (saliva) and the mucolytic agent for pretreatment is moved to the PCR test device to perform the PCR test. FIG. 12 shows an outline of the operation sequence of the PCR test preprocessing automation device as described above. In FIG. 12, * 1 is performed by a transfer robot, * 2 is performed by a lid attachment / detachment device, * 3 is performed by a cap attachment / detachment device, and * 4 is performed by a solubilizer charging device.

FIG. 13 is a diagram showing the concept of an example of an information management system in a PCR test. A barcode 33 reflecting subject information is affixed to the saliva collection container 24, and the barcode 33 is read by the barcode reader 34 and transmitted to the information management device 40. On the other hand, a bar code 38 is affixed to the PCR test container 25, and when a sample (salmon) is charged from the saliva collection container 24 into the PCR test container 25 by the transfer robot 22, the bar code reader 39 is used to bar. The code 38 is read and transmitted to the information management device 40, and the information of the barcode 33 and the information of the barcode 38 are associated with each other. The above is executed in the PCR test preprocessing automation device 21. In the subsequent PCR inspection device 50, the barcode 38 attached to the PCR inspection container 25 is read by the barcode reader 51 and transmitted to the information management device 40, and the inspection result and the like are associated with the subject information.

The barcode 33 may be attached to the upper surface of the lid 8 instead of the side surface of the sample (saliva) collection container 24. In this case, the barcode reader 34 can be provided on the lid attachment / detachment device (not shown). Further, the barcode 38 may be attached to the upper surface of the cap (not shown) instead of the side surface of the PCR test container 25. In this case, the barcode reader 39 can be provided in the cap attachment / detachment device (not shown).

INDUSTRIAL APPLICABILITY The present invention is useful as a device that automatically performs pretreatment of a saliva collection container in a saliva PCR test system before a PCR test.

1 Saliva collection container 2 Closed bottom 3, 3a Open top 4, 4a Container 5 First tilt 6 Second tilt 7, 7a First recess 8 Lid 9, 9a Second recess 10 Mouthpiece 15 Mouthpiece 21 PCR test preprocessing automation device 22 Transfer robot 23 Transfer syringe 24 Saliva collection container 25 PCR test container 33 Bar code 34 Bar code reader 36 Liquid leak detector 37 Camera 38 Bar code 39 Bar code reader 40 Information management device 50 PCR Inspection device 51 Bar code reader

Claims (3)

It is equipped with a transfer robot capable of moving in three-dimensional directions, a transfer syringe, a saliva collection container, and a PCR test container in which a mucus lysing agent for pretreatment is pre-loaded.
Grab the transfer syringe using the transfer robot and move it to the saliva collection container, suck saliva from the saliva collection container with the transfer syringe, and move the transfer syringe that sucked the saliva to the PCR test container using the transfer robot. Then, a PCR test pretreatment automation device characterized in that saliva is poured from a transfer syringe into a PCR test container to perform PCR test pretreatment. It is equipped with a transfer robot capable of moving in three dimensions, a transfer syringe, a saliva collection container, a PCR test container, and a solubilizer input device for charging a mucus lysing agent for pretreatment.
Grab the transfer syringe using the transfer robot and move it to the saliva collection container, suck saliva from the saliva collection container with the transfer syringe, and use the transfer robot to suck the saliva into the transfer syringe by the dissolving agent injection device. The PCR test pretreatment is characterized by moving to a PCR test container in which a mucilage dissolving agent for pretreatment is charged in advance, and saliva is poured from a transfer syringe into the PCR test container to perform the PCR test pretreatment. Automation device. The first barcode to be associated with the subject information attached to the saliva collection container, the first barcode reader to read the information of the first barcode, and the second barcode to identify the container attached to the PCR test container. , A second barcode reader that reads information on the second barcode, an information management device that has a function of linking information of the first barcode and information of the second barcode, and a function of linking PCR test results and subject information. The PCR test preprocessing automation device according to claim 1 or 2, wherein the device comprises.

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