JP2021088524A - Liposome composition, skin external preparation containing the same, and method for producing liposome composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a liposome composition having an excellent skin improving effect by further enhancing the permeability to the skin, a skin external preparation containing the same, and a method for producing the liposome composition. SOLUTION: This liposome composition contains (a) phospholipid, (b) polyhydric alcohol, (c) fat and oil, (d) fermented rice liquor with yeast, and (e) vitamin C derivative as components, and is a liposome. Is formed. This liposome composition comprises a step of mixing and heating a raw material containing (a) phospholipid, (b) polyhydric alcohol, and (c) fat and oil to obtain a first raw material liquid, and (d) rice fermentation with yeast. A step of dissolving (e) a substance having a skin improving effect containing a vitamin C derivative in a liquid to obtain a second raw material liquid, and adding and mixing the second raw material liquid with the first raw material liquid for high-pressure emulsification. It can be manufactured by the process of processing. The external preparation for skin contains the above-mentioned liposome composition. [Selection diagram] Fig. 1

Description

The present invention relates to a liposome composition suitable for external preparations for skin such as cosmetics, an external preparation for skin containing the same, and a method for producing a liposome composition.

In recent years, a composition containing liposomes composed of lipid phosphates having a hydrophilic group and a novel oily group and organized as monolayered or multilayer vesicles has been blended into pharmaceuticals, quasi-drugs, cosmetics and the like. Various products have been proposed in which an active ingredient that causes healing or improvement in the health of the human body is allowed to permeate and be stored in the body through the surface, which is the boundary with the outside world of the human body, particularly the skin.

As such a prior art, in Patent Document 1 below, glycosphingolipids obtained from plant materials such as rice bran and rice germ are encapsulated in nanoliposomes or made into nanodisperse aqueous solutions, and such nano-prepared preparations are applied to the skin. It is described that the barrier function and the water retention function of the skin are efficiently exerted by increasing the endogenous ceramide in the skin.

Further, Patent Document 2 below contains (a) phospholipid, (b) cholesterol and / or phytosterol, (c) platinum group metal colloid, and (d) water as components, and the component (c) is a component. Described is a liposome composition characterized by being encapsulated in a liposome formed by (a) and component (b).

Japanese Unexamined Patent Publication No. 2012-184166 Japanese Unexamined Patent Publication No. 2008-266324

However, the technique of Patent Document 1 uses glycosphingolipids obtained from plant materials such as rice bran and rice germ, and does not pay attention to the water-soluble active ingredient contained in rice. Further, in the technique of Patent Document 2, water is used as a dispersion medium, and there is room for further improvement in terms of permeability to the skin.

Therefore, an object of the present invention is to provide a liposome composition having an excellent skin improving effect by further enhancing the permeability to the skin, a skin external preparation containing the same, and a method for producing the liposome composition. ..

As a result of diligent research to achieve the above object, the present inventors have found that liposomes having a small average particle size can be stably formed by using a rice fermentation broth made from yeast as a dispersion medium. It came to be completed.
That is, the liposome composition of the present invention contains the following components (a) to (f):
(A) Phosphorus (b) Polyhydric alcohol (c) Oil (d) Rice fermentation with yeast (e) Vitamin C derivatives are contained.
It is characterized in that liposomes are formed.

In the liposome composition of the present invention, the average particle size of the liposomes is preferably 80 to 150 nm.
The liposome composition of the present invention preferably contains hydrogenated lecithin and lysophosphatidic acid as the (a) phospholipid.

In the liposome composition of the present invention, it is preferable that the vitamin C derivative (e) is 3-O-ethylascorbic acid and the pH of the liposome composition is 3.0 to 5.0.

The liposome composition of the present invention preferably further contains (f) sterols and vitamin E.

The liposome composition of the present invention further comprises (g) Ryokuto growing point cultured cell extract, (h) apple fruit cultured cell extract, (i) white jellyfish polysaccharide, (j) low molecular weight hyaluronic acid, and (k) wild thyme extract. It is preferable to contain at least one selected from.

The external preparation for skin of the present invention is characterized by containing any of the above-mentioned liposome compositions.

The external preparation for skin of the present invention is preferably used for improving the moisturizing property of the skin.

The external preparation for skin of the present invention is preferably used for improving the elasticity of the skin.

The external preparation for skin of the present invention is preferably used for improving the texture of the skin.

The method for producing a liposome composition of the present invention includes a step of mixing a raw material containing (a) a phospholipid, (b) a polyhydric alcohol, and (c) an oil and fat, and heating and dissolving the mixture to obtain a first raw material solution.

(D) A step of dissolving a substance having a skin improving effect, including a vitamin C derivative, in a rice fermented liquid made of yeast to obtain a second raw material liquid.
It is characterized by including a step of adding and mixing the second raw material liquid with the first raw material liquid to perform an emulsification treatment.

In the method for producing a liposome composition of the present invention, it is preferable that the emulsification treatment is performed by a high-pressure emulsification treatment.

In the method for producing a liposome composition of the present invention, it is preferable to use hydrogenated lecithin and lysophosphatidic acid as the phospholipid (a).

In the method for producing a liposome composition of the present invention, it is preferable that the vitamin C derivative (e) is 3-O-ethylascorbic acid and the pH of the liposome composition is adjusted to 3.0 to 5.0.

In the method for producing a liposome composition of the present invention, it is preferable that (f) sterol and vitamin E are further contained in the step of obtaining the first raw material solution.

In the method for producing the liposome composition of the present invention, in the step of obtaining the second raw material solution, as the substance having the skin improving action, (g) Ryokuto growing point cultured cell extract, (h) apple fruit cultured cell extract, It is preferable to use at least one selected from (i) white jellyfish polysaccharide, (j) low molecular weight hyaluronic acid, and (k) wild thyme extract.

According to the present invention, by using a rice fermented solution made of yeast as a dispersion medium, liposomes having a small average particle size can be stably formed, whereby the skin of a substance having a skin improving effect such as a vitamin C derivative can be formed. It is possible to enhance the penetration into the skin and enhance the skin improving effect.

It is a photograph by a transmission electron microscope of 200,000 times of the liposome composition obtained in the Example of this invention.

The liposome composition of the present invention contains the following components (a) to (f):
It is characterized by containing (a) phosphorus fat (b) polyhydric alcohol (c) oil (d) rice fermentation with yeast (e) vitamin C derivative, and liposomes are formed.

The component (a) phospholipid used in the present invention has a structure in which fatty acid and phosphoric acid are bonded to phosphoric acid with glycerin and sphingosine as the central skeleton, and alcohol is ester-bonded to phosphoric acid. There are different types of. Phospholipids are contained as main components that form the lipid bilayer membrane of the liposome composition. Such component (a) phospholipid is not particularly limited as long as it is used in ordinary cosmetics, and for example, phosphatidylcholine (that is, lecithin), phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol. , Glycerophospholipids such as diphosphatidylglycerol and phosphatidylic acid, and lysophosphatidic acid such as lysophosphatidic acid. In the present invention, it is particularly preferable to contain hydrogenated lecithin and lysophosphatidic acid. Lysophosphatidic acid is a component that induces the new expression of capillaries and supports healthy texture construction from the deep part of the skin. It also has the ability to increase ceramide production and regulate the barrier function. It can also be expected to have the effect of keeping the water retention of the skin and making pores inconspicuous.

Ingredient (b) The polyhydric alcohol is not particularly limited as long as it is used in ordinary cosmetics, and examples thereof include glycerin, propanediol, propylene glycol, butylene glycol, pentylene glycol, and hexylene glycol. , These can be used alone or in combination of two or more. In particular, glycerin, propanediol, butylene glycol, and pentylene glycol are preferably used.

The oil and fat of the component (c) is not particularly limited as long as it is used in ordinary cosmetics, and for example, avocado oil, flaxseed oil, camellia oil, almond oil, southern ka oil, macadamia nut oil, corn oil, and kyonin oil. , Grape seed oil, medfoam oil, jojoba oil, olive oil, sunflower oil, rapeseed oil, cinnamon oil, egg yolk oil, sesame oil, wheat germ oil, southern ka oil, sunflower oil, saflower oil, cottonseed oil, soybean oil, Pepper oil, tea seed oil, kaya oil, rice germ oil, rice bran oil, cacao butter, palm oil, evening primrose oil, shea butter, hydrogenated palm oil, palm oil, palm kernel oil, mokuro kernel oil, hydrogenated castor oil, etc. Can be mentioned. In addition, waxes such as lanolin acetate, liquid lanolin, sugarcane wax, reduced lanolin, POE lanolin alcohol ether, and carbonization of liquid paraffin, ozokelite, squalane, paraffin, isoparaffin, octane, decane, dodecane, isododecane, hexadecane, isohexadecan, etc. Hydrogen, lauric acid, myristic acid, palmitic acid, stearic acid, behen (behenin) acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tollic acid, isostearic acid, linoleic acid, linoleic acid, eikosapentaenoic acid (EPA ), Docosahexaenoic acid (DHA), arachidonic acid and other higher fatty acids can also be used. In the present invention, it is particularly preferable to use hydrogenated castor oil, squalane, sugar squalane, oleyl glyceryl, and soybean sterol.

Component (d) The fermented rice liquor with yeast is a component that forms the aqueous phase of the liposome and serves as a dispersion medium existing around the liposome. The rice fermented broth is obtained by culturing yeast in a medium containing a raw material derived from rice and collecting the yeast from the culture broth. Examples of the rice-derived raw material include whole grain rice flour, rice flour, rice bran, germinated brown rice, and rice jiuqu, and rice jiuqu is particularly preferably used. The yeast may be any commonly used yeast, and for example, yeast belonging to the genus Saccharomyces, the genus Schizosaccharomyces, or the like can be used. Of these, yeast belonging to the genus Saccharomyces is preferable, and Saccharomyces Veronae is particularly preferable. Rice fermented liquid includes, for example, the product name "Rice Extract Koken BG" (manufactured by Koken Co., Ltd.), the product name "Kome Extract Koken MPN" (manufactured by Koken Co., Ltd.), and the product name "Rice Fermented Liquid PD" (Maruzen Pharmaceuticals Co., Ltd.). Commercial products such as (manufactured by the company) can also be used. The fermented rice liquor contains abundant nutritional components such as various water-soluble amino acids, oil-soluble amino acids, trace minerals, and lactic acid, and can enhance the texture, firmness, and elasticity of the skin.

Ingredient (e) Vitamin C derivatives are derivatives in which vitamin C (L-ascorbic acid) is improved in order to enhance product stability and skin permeability, and are, for example, 3-O-ethylascorbic acid and ascorbyl palmitate. Examples thereof include trisodium phosphate, sodium ascorbyl phosphate, ascorbic magnesium phosphate, and 2-O-ethylascorbic acid, and 3-O-ethylascorbic acid is particularly preferably used. Vitamin C derivatives have a whitening effect on the skin, a direct reduction of melanin, and an effect of promoting epidermal hyaluronic acid production.

The liposome composition of the present invention preferably further contains the component (f) sterol and vitamin E. Examples of sterols include animal sterols, plant sterols (phytosterols), fungal sterols and the like. Animal sterols include, for example, cholesterol, cholestanol, and 7-dehydrocholesterol. Examples of plant sterols (phytosterols) include sitosterol, stigmasterol, fucosterol, spinasterol, and brassicasterol. In addition, phytostanol, which is a hydrogenated additive of plant sterols, can be mentioned. Examples of the fungal sterol include ergosterol. As the sterol used in the present invention, a commercially available commercially available product can be used. For example, the product name "Phytosterol S" (phytosterol) manufactured by Tama Biochemical Co., Ltd., the product name "Cholesterol JSCI" (cholesterol) manufactured by Nippon Seika Co., Ltd., and the product name "GENEROL 122N" (phytosterol) manufactured by Cognis Japan Co., Ltd. ), Product names manufactured by Lipid, such as "LIPOID P75, LIPOID P100".

The liposome composition of the present invention further comprises (g) Ryokuto growing point cultured cell extract, (h) apple fruit cultured cell extract, (i) white jellyfish polysaccharide, (j) low molecular weight hyaluronic acid, and (k) wild thyme extract. It is preferable to contain at least one selected from.

Ingredient (g) Mung bean growth point Mung bean (green beans) refers to seeds that can be made into legumes called Yaenari (Yaesei), and is said to have been edible in Japan for a long time. Mung bean growth point cultured cell extract is a stem cell extract extracted and cultured from mung bean (mung bean) stem cells, exerts a high aging care effect, reduces light damage, and suppresses inflammation that attacks cells. The mung bean growth point cultured cell extract is commercially available, and for example, the trade name "Plant C Stem" of Innovacos can be used.

Ingredient (h) Apple fruit cultured cell extract is an extract obtained by culturing stem cells of the Swiss apple variety Uttwiler Spatolauber, which has excellent characteristics such as resistance to swelling and long-term storage, and is used as epidermal stem cells. It has an anti-aging effect that nourishes and helps the ability to regenerate the epidermis. The apple fruit cultured cell extract is commercially available, and for example, the trade name "Phytoceltec Mars domestica" manufactured by H. Holstein Co., Ltd. can be used.

Ingredient (i) The white fungus polysaccharide is a purely natural vegetable acidic polysaccharide with high moisturizing power extracted from the white fungus. Since the molecular weight is as high as 1 million or more, high viscosity and moisturizing effect can be expected. The white fungus polysaccharide is commercially available, for example, manufactured by Nippon Seika Co., Ltd., trade name "Tremoist-SL, Tremoist-SLB, Tremoist-TP", manufactured by Oriza Yuka Co., Ltd., trade name "White fungus polysaccharide-P". Can be used.

Ingredient (j) low molecular weight hyaluronic acid (oligo hyaluronic acid) not only covers the skin surface but also deeply penetrates into the stratum corneum, thereby contributing to moisture from the inside of the skin. Low-molecular-weight hyaluronic acid (oligo-hyaluronic acid) permeates the keratin and stays for a long time, so that the moisturizing power is maintained. Low-molecular-weight hyaluronic acid (oligohyaluronic acid) is commercially available, and for example, the trade name "hyalooligo R" manufactured by Kewpie Corp. can be used.

Ingredient (k) Wild thyme extract is an extract obtained by extracting from the whole plant of the Labiatae plant Wild thyme (scientific name: Thymus serpyllum English name: Creeping thyme) with water, ethanol, 1,3-butylene glycol or a mixture thereof. Is. It has an excellent effect of suppressing the protein "Xinen" that transports melanin to epidermal cells and surface it, and exerts a whitening effect by suppressing the transport of melanin.

In addition to the above compounds, the liposome composition of the present invention can usually contain components that can be used in the external composition for skin as long as the effects of the present invention are not impaired. For example, surfactants, greasing agents, silicones, moisturizers, polymers, UV absorbers, metal sequestering agents, solvents, pH regulators, vitamins, antioxidants, antioxidants, preservatives, fragrances. And so on.

The pH of the liposome composition of the present invention is preferably 3.0 to 8.0, more preferably 3.0 to 5.0.

The liposome composition of the present invention can stably form liposomes having a small average particle size by using a fermented rice solution as a dispersion medium. The average particle size of the liposomes is preferably 50 to 500 nm, more preferably 60 to 150 nm. When the average particle size of the liposomes is in the above range, it is possible to enhance the permeability of substances having a skin improving effect such as vitamin C derivatives to the skin. The average particle size of the liposome can be measured using a laser diffraction type particle size distribution measuring device, for example, the trade name "SALD-7500" (manufactured by Shimadzu Corporation), and can be obtained as the median diameter.

The external preparation for skin of the present invention is characterized by containing the above-mentioned liposome composition. Examples of the external skin preparation of the present invention include lotion, milky lotion, cream, beauty essence, gel cosmetic, facial mask, hair restorer, sunscreen cosmetic, foundation cosmetic, liquid foundation, cream foundation, hair treatment, etc. Examples include hair rinses and body cosmetics.

The content of the component (a) phospholipid in the external preparation for skin is preferably 0.1 to 1.0% by mass, more preferably 0.1 to 0.5% by mass. The content of the component (b) polyhydric alcohol is preferably 1 to 20% by mass, more preferably 1 to 8% by mass. The content of the component (c) fat and oil is preferably 0.05 to 30.0% by mass, more preferably 0.05 to 10.0% by mass. Further, the content of the rice fermentation broth produced by the component (d) yeast is preferably 40 to 99.0% by mass, more preferably 80 to 99.0% by mass. Furthermore, the content of the component (e) vitamin C derivative is preferably 0.1 to 5.0% by mass, more preferably 0.3 to 2.0% by mass.

As shown in the test examples described later, the external preparation for skin of the present invention is excellent in improving the moisturizing property of the skin, improving the elasticity of the skin, and improving the texture of the skin, and is used for improving the moisturizing property of the skin and the elasticity of the skin. It can be used as a composition for improving the sex and improving the texture of the skin.

The amount of the external preparation for skin of the present invention to be used per day for an adult is preferably 1 to 10 g, more preferably 1 to 3 g, for example, in terms of the amount used per area of the entire face.

Next, the method for producing the liposome composition of the present invention includes a step of mixing and heating a raw material containing (a) phospholipid, (b) polyhydric alcohol, and (c) fat and oil to obtain a first raw material liquid. , (D) A step of dissolving a substance having a skin improving effect containing a vitamin C derivative in a rice fermented liquid containing yeast to obtain a second raw material liquid, and the second raw material liquid in the first raw material liquid. Is included, and the step of emulsifying the mixture is included.

In the step of obtaining the first raw material liquid, for example, a raw material containing (a) phospholipid, (b) polyhydric alcohol, and (c) fat and oil is put into a stirrer such as a propeller stirrer, and at 80 to 85 ° C., 10 This can be done by stirring for ~ 15 minutes.

In the step of obtaining the second raw material liquid, (d) a rice fermented liquid containing yeast, (e) a substance having a skin improving effect including a vitamin C derivative, and other water-soluble raw materials are used in a stirrer such as a propeller stirrer. It can be carried out by charging and stirring and dissolving. The step of obtaining the second raw material liquid can be divided into several raw materials and performed in a plurality of steps.

The step of adding and mixing the second raw material liquid with the first raw material liquid and performing the emulsification treatment can be carried out by, for example, a high-pressure emulsification treatment, an ultrasonic treatment method, a freeze-thaw method, or the like. When performing high-pressure emulsification treatment, for example, "Nanomizer" or "NanoVeta" (trade name, manufactured by Nanomizer Co., Ltd.) or "Microfluidizer" (trade name, manufactured by Paulec Co., Ltd.) is used, preferably with a pressure of 50 Mpa ~. It can be carried out under the condition of 200 Mpa. When performing ultrasonic processing, for example, "Handheld ultrasonic device UP50H (50W, 30kHz)" (trade name, manufactured by BRANSON), "VialTweeter ultrasonic reactor UTR200" (trade name, manufactured by hielScher Ultrasound Technology) Can be used for preparation. In this case, as described above, the step of obtaining the second raw material liquid may be divided into several raw materials and performed in a plurality of steps, and each raw material may be sequentially added to the first raw material liquid.

<Example 1> (Production of beauty essence)
A liposome composition was prepared by the formulation shown in Table 1 below.

手順としては、まず、グリセリン、ペンチレングリコールを、プロペラ攪拌機を有するミキサーに投入して攪拌混合し、これに、水添加レシチン、リゾホスファチジン酸を添加混合して、加熱溶解した。
更に、上記混合液に、オクチルドデカノール、スクワラン、オレイルグリセリルエーテル、フィトステロール、水添ヒマシ油、トコフェロールを添加混合して、８０℃で加熱溶解し、第１原料液を得た。

As a procedure, first, glycerin and pentylene glycol were put into a mixer having a propeller stirrer and stirred and mixed, and then water-added lecithin and lysophosphatidic acid were added and mixed, and the mixture was heated and dissolved.
Further, octyldodecanol, squalane, oleylglyceryl ether, phytosterol, hydrogenated castor oil, and tocopherol were added to and mixed with the above mixed solution, and the mixture was heated and dissolved at 80 ° C. to obtain a first raw material solution.

Next, in the rice fermented liquor, amino acid mixture, apple fruit cultured cell extract, rhododendron fergineum leaf cultured cell extract, adenosine triphosphate 2Na, wild thyme extract, 1,3 butylene glucol, phenoxyethanol, white jellyfish polysaccharide, Hyalo-oligosaccharide, 3-O-ethylascorbic acid, and citric acid were added and mixed, and the mixture was heated and dissolved at 80 to 85 ° C. to obtain a second raw material solution.

The second raw material liquid was added to the first raw material liquid, and a high-pressure emulsification treatment was carried out at 50 to 150 mPa using a "nanomizer" (trade name, manufactured by Nanomizer Co., Ltd.) to obtain a liposome composition. A 200,000x transmission electron micrograph of this liposome composition is shown in FIG. As shown in this photograph, it can be seen that this liposome composition has liposomes organized as monolayered or multilayer vesicles. The average particle size (median diameter) measured using the laser diffraction type particle size distribution measuring devices "SALD-7500" and "SALD-7100" (manufactured by Shimadzu Corporation) was in the range of 60 to 150 nm. ..

On the other hand, the compounding raw materials shown in Table 2 below were dissolved by heating and stirring at 80 ° C. to prepare a viscosity adjusting solution.

次に、表１の配合からなるリポソーム組成物と、表２の配合からなる粘度調整液とを、質量比で２．５：１．０の割合で混合して、美容液を製造した。

Next, the liposome composition composed of the formulations shown in Table 1 and the viscosity adjusting solution comprising the formulations shown in Table 2 were mixed at a mass ratio of 2.5: 1.0 to produce a beauty essence.

<Test example>
The beauty essence obtained in Example 1 was used by a total of 22 Japanese women, 11 from 40 to 49 years old and 11 from 50 to 59 years old, before the start of use and 4 weeks after the start of use. After that, the condition of the skin was observed, and the skin moisture content, skin elasticity, and texture replica image were numerically analyzed.

The skin water content was measured using a skin measuring instrument "Corneometer CM825" (trade name, manufactured by Integral Co., Ltd.) at three locations on the cheekbone and 5 mm to the left and right, and expressed as the average value.

For skin elasticity, use the skin viscoelasticity measuring device "Cute Meter MPA580" (trade name, manufactured by Integral Co., Ltd.), which is 4 cm from the base of the earlobe on the straight line connecting the base of the earlobe and the edge of the lips. The site was measured three times, and the maximum amplitude, minimum amplitude, and return rate of skin elasticity were determined by statistical analysis.
Numerical analysis of the texture replica image involves connecting the base of the earlobe and the nose, collecting a replica 7 cm from the base of the earlobe, and using the statistical P test to determine the texture volume fraction, texture average depth, and texture number. It was measured.

The texture image was taken by connecting the base of the earlobe and the nose, and using a 60x magnifying camera, a reflected image was taken at a portion 7 cm from the base of the earlobe.

The transition of skin water content is shown in Table 3 below.

平均値±標準偏差 ｎ＝２２
塗布前と塗布４週間後を対応のある検体で統計解析を行った。
＊：P＜0.05 ＊＊：P＜0.01 ＊＊＊：P＜0.001

Mean ± standard deviation n = 22
Statistical analysis was performed on the corresponding samples before and 4 weeks after application.
*: P <0.05 **: P <0.01 ***: P <0.001

The transition of skin elasticity is shown in Table 4 below.

平均値±標準偏差 ｎ＝２２
塗布前と塗布４週間後を対応のある検体で統計解析を行った。
＊：P＜0.05 ＊＊：P＜0.01 ＊＊＊P＜0.001
表４に示されるように、塗布前よりも塗布後の方が、皮膚弾力性の振幅最大値、振幅最小値も小さくなっており、また、戻り率も大きくなっているので、皮膚の弾力性が向上したことがわかる。

Mean ± standard deviation n = 22
Statistical analysis was performed on the corresponding samples before and 4 weeks after application.
*: P <0.05 **: P <0.01 *** P <0.001
As shown in Table 4, the maximum amplitude value and the minimum amplitude value of the skin elasticity are smaller after the application than before the application, and the return rate is also larger, so that the elasticity of the skin is large. Can be seen to have improved.

Table 5 below shows the transition of texture replica image / numerical analysis.

平均値±標準偏差 ｎ＝２２
塗布前と塗布４週間後を対応のある検体で統計解析を行った。
＊：P＜0.05 ＊＊：P＜0.01 ＊＊＊：P＜0.001
表５に示されるように、塗布前よりも塗布４週間後の方が、キメ体積率、キメ平均深度、キメ個数が大きくなっており、肌のキメが細かくなっていることがわかる。

Mean ± standard deviation n = 22
Statistical analysis was performed on the corresponding samples before and 4 weeks after application.
*: P <0.05 **: P <0.01 ***: P <0.001
As shown in Table 5, it can be seen that the texture volume ratio, the average texture depth, and the number of textures are larger after 4 weeks of application than before application, and the texture of the skin is finer.

Claims (16)

The following components (a) to (f):
(A) Phosphorus (b) Multivalent alcohol (c) Oil (d) Fermented rice with yeast (e) Contains vitamin C derivatives
A liposome composition characterized in that liposomes are formed. The liposome composition according to claim 1, wherein the liposome has an average particle size of 80 to 150 nm. The liposome composition according to claim 1 or 2, which contains hydrogenated lecithin and lysophosphatidic acid as the phospholipid (a). The liposome composition according to any one of claims 1 to 3, wherein the vitamin C derivative (e) is 3-O-ethylascorbic acid, and the pH of the liposome composition is 3.0 to 5.0. Stuff. The liposome composition according to any one of claims 1 to 4, further comprising (f) sterol and vitamin E. Further, at least one selected from (g) Ryokuto growth point cultured cell extract, (h) apple fruit cultured cell extract, (i) white jellyfish polysaccharide, (j) low molecular weight hyaluronic acid, and (k) wild time extract. The liposome composition according to any one of claims 1 to 5, which is contained. An external preparation for skin, which comprises the liposome composition according to any one of claims 1 to 6. The external preparation for skin according to claim 7, which is used for improving the moisturizing property of the skin. The external preparation for skin according to claim 7, which is used for improving the elasticity of the skin. The external preparation for skin according to claim 7, which is used for improving the texture of the skin. A step of mixing raw materials containing (a) phospholipids, (b) polyhydric alcohols, and (c) fats and oils and heating and dissolving them to obtain a first raw material liquid, and (d) a rice fermented liquid with yeast, and (e) vitamins. A step of dissolving a substance having a skin improving effect including a C derivative to obtain a second raw material liquid, and
A method for producing a liposome composition, which comprises a step of adding and mixing the second raw material liquid with the first raw material liquid to perform an emulsification treatment. The method for producing a liposome composition according to claim 11, wherein the emulsification treatment is performed by a high-pressure emulsification treatment. The method for producing a liposome composition according to claim 11 or 12, wherein hydrogenated lecithin and lysophosphatidic acid are used as the phospholipid (a). The liposome according to any one of claims 11 to 13, wherein the vitamin C derivative (e) is 3-O-ethylascorbic acid, and the pH of the liposome composition is adjusted to 3.0 to 5.0. A method for producing a composition. The method for producing a liposome composition according to any one of claims 11 to 14, further comprising (f) sterol and vitamin E in the step of obtaining the first raw material liquid. In the step of obtaining the second raw material solution, as the substance having the skin improving action, (g) Ryokuto growth point cultured cell extract, (h) apple fruit cultured cell extract, (i) white jellyfish polysaccharide, (j) low The method for producing a liposome composition according to any one of claims 11 to 15, wherein at least one selected from molecular hyaluronic acid and (k) wild thyme extract is used.

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