JP2021065834A - Bottom sediment environmental improvement material - Google Patents

Abstract

To provide a bottom sediment environmental improvement material which causes no change in quality in distribution and storage, is excellent in handling property, is hardly flown away with a water flow, exhibits a part where useful bacterial are aggregated with a comparatively high concentration in a bottom in a timed manner, and then is spread over the bottom and can give an improvement effect over a wide range.SOLUTION: A bottom sediment environmental improvement material stores liquid wetting peat moss obtained by impregnating 15-25 pts.wt. of dry peat moss with a bottom sediment improving microbial fermentation liquid in a supersaturated state, and 150-155 pts.wt. of particulate steel slag, in a mesh-like bag formed of a biodegradable resin.SELECTED DRAWING: None

Description

The present invention relates to materials for improving the environment of sediments such as seas, lakes, ponds and rivers.

It is known that the condition of the bottom of water such as the sea, lakes, ponds and rivers has a great influence on various fisheries including sea surface fisheries and inland fisheries.

In particular, when sludge of the bottom sediment progresses, the oxygen supply is insufficient and putrefaction progresses, causing bad odors such as hydrogen sulfide odor, causing landscape problems such as discoloration of the sea surface, and further making it difficult for ships to navigate. Sometimes.

Therefore, dredging work may be carried out to improve the sediment environment, but it is difficult to fundamentally improve the sediment environment because sludge accumulates again in the dents where sludge has been dredged. The current situation.

Therefore, in recent years, a sediment environment improving material configured to improve the sediment environment by submerging the microbial material in a water area has been proposed (see, for example, Patent Document 1).

According to such a sediment environment improving material, new environment improving microorganisms can be engrafted on the bottom, and the sediment can be improved.

Japanese Unexamined Patent Publication No. 2016-609594

However, since the above-mentioned conventional sediment environment improving material uses live bacteria, there is a problem that the quality is liable to change because the reproduction and fermentation proceed even during distribution and storage.

In addition, there is also a problem that it is difficult to keep the material in the part where the bottom sediment is to be improved because the material moves due to the water flow such as the ebb and flow of the tide and the flow of the river. However, even in this case, it is more desirable if the materials can be dissipated because it is desired to spread the environmental improvement effect of the sediment over as wide a range as possible after the lapse of a predetermined period.

The present invention has been made in view of such circumstances, and the quality does not easily change during distribution or storage, the handling is excellent, the water flow does not easily wash away, and the useful bacteria are relatively high. Provided is a sediment environment improving material capable of causing a part gathered at a concentration to appear at the bottom in a timely manner and then diffusing to the bottom to bring about a wide range of improvement effects.

In order to solve the above-mentioned conventional problems, the bottom sediment environment improving material according to the present invention includes liquid wet slag obtained by impregnating 15 to 25 parts by weight of dried moss with a fermentation broth for improving bottom sediment in a hypersaturated state. It was decided to house 150 to 155 parts by weight of granular steel slag in a mesh-shaped bag made of biodegradable resin.

In addition, the sediment-improving microbial fermentation broth contains at least 0.6 parts by weight of dried stevia stalk powder, 0.6 parts by weight of rice bran powder, 0.6 parts by weight of dried oak powder, and salt-tolerant yeast 11 to 13 A static step in which a mixed solution of a weight of steam water and a mixed solution is allowed to stand for a predetermined time, and a mixed solution that has undergone the static step are stored in a predetermined container, and the mixed solution is contained in a container shape. A mixed solution is formed through a container accommodating step of forming a weakly sterilized region which is 10 cm or more from any position on the outer surface and 17 cm or less from at least any position on the outer surface, and the container accommodating step. Place a predetermined container containing the above in a heating space, and raise the temperature of the heating space from normal temperature to about 2.5 atm for 150 to 160 ° C over 45 to 60 minutes at about 2.5 atm. Maintain the state of 150 to 160 ° C for 1 to 3 minutes, then set the heating space to a state of 115 to 125 ° C at about 2 atm for 3 to 5 minutes, and set the temperature to decrease at about 2 atm to 115 to 125 ° C. Is maintained for 20 to 40 minutes, and the temperature of the heating space is lowered to a state of normal temperature and pressure for 24 to 30 hours to leave the heat-selected microorganisms in the mixed solution, and at least the microorganisms A solid-liquid separation step of solid-liquid separation of the mixed solution that has undergone the microorganism selection step by means capable of shifting to a liquid phase to obtain a microorganism-containing liquid, and a solid-liquid separation step of adding a sugar source to the obtained microorganism-containing liquid at normal temperature and pressure. Ferment for a predetermined time, the pH of the microorganism-containing liquid is 4.5 or less, and the oxidation-reduction potential is -100 mV or less. It is also characterized by a second fermentation step in which it is added and fermented until the pH becomes 3.1 or less to obtain a defective fermentation inhibitor, and the fermented liquid obtained obtained.

According to the bottom sediment environment improving material according to the present invention, 15 to 25 parts by weight of dry water moss is impregnated with a bottom sediment improving microbial fermented liquid in a supersaturated state, and 150 to 155 parts by weight of granular water moss. Since the steel slag is housed in a mesh-like bag made of biodegradable resin, the quality does not change easily during distribution and storage, it is excellent in handling, and it is difficult to be washed away by water flow. Makes a site where useful bacteria gather at a relatively high concentration appear at the bottom in a timely manner, and acclimatizes the useful bacteria that can improve the environment at the same site while keeping the predominance, and then spreads to the bottom and improves extensively. It is possible to provide a bottom sediment environment improving material that can bring about an effect.

The present invention relates to a material for improving the environment of bottom sediments such as the sea, lakes, ponds and rivers, and in particular, the quality does not change easily during distribution and storage, is excellent in handling, and depends on the water flow. In addition, a site where useful bacteria are gathered at a relatively high concentration that is difficult to be washed away appears at the bottom in a timely manner, and useful bacteria that can improve the environment at the same site are acclimatized while maintaining predominance, and then spread to the bottom. It provides materials for improving the sediment environment that can bring about a wide range of improvement effects.

That is, the bottom sediment environment improving material according to the present embodiment is characterized in that it is configured by accommodating liquid-wet water moss and granular steel slag in a mesh-shaped bag.

Here, in the present specification, bottom sediment improvement means improving the ORP value of water (hereinafter, also referred to as sample water) collected from the bottom soil of the target water bottom region for which the bottom sediment environment is to be improved. The main goal is to set the ORP value higher than the ORP value before the treatment, and to lower the concentration of hydrogen sulfide when it is detected, than the concentration before the improvement treatment.

To give an example, an ORP having a negative ORP value, for example, a bottom region where sample water of -500 to -150 mV has been separated, has a higher ORP value, preferably a positive value, for example, an ORP of 100 mV to 200 mV. The bottom region where the sample water showing the value is separated, or the bottom region where the sample water where 2 to 5 ppm of hydrogen sulfide is detected is separated is detected at a lower value, preferably 0.5 ppm or less. Or, it can be said that the water bottom region is not detected as being below the detection limit of the measuring device.

Here, the liquid-wet water moss is obtained by impregnating 15 to 25 parts by weight of dry water moss with a bottom sediment-improving microbial fermentation liquid in a supersaturated state.

As the dried water moss, dried water moss generally used for gardening can be used. As an example, dried products of aquatic plants classified in the family Sphagnaceae can be adopted.

The sediment-improving microbial fermented liquid is a microorganism capable of aerobically decomposing excess organic matter existing in the target region for improving the sediment environment, for example, a sludge-like region, to improve the sediment environment (hereinafter, sediment improvement). It is a liquid that is fermented by (also referred to as microorganisms) and contains metabolites of sludge-improving microorganisms, and functions as a starter for forming a flora of sludge-improving microorganisms on the bottom of the water while using dry aquatic plants as a foothold.

The type of the bottom sediment improving microorganism is not particularly limited, but for example, microorganisms selected according to the screening method described later from the group of microorganisms contained in the brackish water collected from the brackish water region described later, particularly favorable. It is desirable to use a bacterial flora rich in salt-resistant (salt-tolerant) yeast.

That is, the bottom sediment-improving microbial fermentation liquid is not particularly limited as long as it contains the fermentation metabolites produced by the fermentation of the above-mentioned bottom sediment-improving microorganisms and has an increased bacterial vigor and amount. It is possible to adopt the liquid produced through the predetermined standing step, the container containing step, the microorganism selection step, the solid-liquid separation step, the first fermentation step, and the second fermentation step described in 1. it can. Through these steps, a microbial flora that excels in decomposing organic substances on the bottom of the water by coexisting with brackish water-derived halophilic (salt-tolerant) yeast and other microbial groups derived from raw materials (for example, lactic acid bacteria group). Contributes to the efficient improvement effect of sediment.

Here, the standing step contains at least a material that is a raw material for extracting the extract contained in the defective fermentation inhibitor (hereinafter, also collectively referred to as an extract extraction material) and a salt-philic (salt-tolerant) yeast. It is a process of mixing brackish water and eluting the extract into brackish water while allowing it to stand. 0.6 parts by weight of dried stevia stem powder, 0.6 parts by weight of rice bran powder, and 0.6 parts by weight of dried oak powder. This is a step of allowing a mixed solution of 11 to 13 parts by weight of brackish water containing at least salt-philic (salt-tolerant) yeast to stand for a predetermined time. In this standing step, these extract extraction materials and brackish water are mixed in predetermined amounts and allowed to stand at room temperature for a predetermined time, for example. The time required for standing can be, for example, 15 to 20 days.

Further, in the container accommodating step, the mixed solution that has undergone the standing step is accommodated in a predetermined container, and the contained mixed solution is 10 cm or more from any position on the outer surface of the accommodating shape of the mixed solution. Moreover, it is a step of forming a weakly sterilized region of 17 cm or less from at least any position on the outer surface so that complete sterilization is not performed when heated in the microorganism selection step described later.

Further, in the microorganism selection step, a predetermined container containing the mixed solution is placed in the heating space through the container storage step, and the heating space is changed from a normal temperature and pressure state to a state of 150 to 160 ° C. at about 2.5 atm. The temperature is raised over 45 to 60 minutes, and the temperature is maintained at 150 to 160 ° C at about 2.5 atm for 1 to 3 minutes to perform the first heating step, and then the heating space is set to 115 to 125 ° C at about 2 atm. It takes 3 to 5 minutes to lower the temperature until it reaches the state, and the state of 115 to 125 ° C is maintained at about 2 atm for 20 to 40 minutes to perform the second heating step, and the heating space is further brought to the state of normal temperature and pressure. This is a step of lowering the temperature over 24 to 30 hours to perform a temperature lowering step, and to leave the heat-selected microorganisms in the mixed solution.

Further, the solid-liquid separation step is a step of obtaining a microorganism-containing liquid by solid-liquid separation of the mixed liquid that has undergone the microorganism selection step by at least a means capable of transferring the microorganism to the liquid phase. The solid-liquid separation does not necessarily have to completely remove the solid content, and is an image of roughly removing the solid content while transferring the selected microorganisms to the liquid phase to improve the fluidity. Such solid-liquid separation can be performed by storing the mixed liquid in a cloth bag or the like and using the dehydration function of the washing machine or the like. Alternatively, a general solid-liquid separation device may be used to apply centrifugal force sufficient to leave microorganisms in the liquid phase to perform solid-liquid separation, or the mixed solution may be contained in a cloth bag and squeezed. A liquid phase may be obtained by squeezing.

In the first fermentation step, a sugar source is added to the obtained microbial-containing liquid and fermented at normal temperature and pressure for a predetermined time, the pH of the microbial-containing liquid is 4.5 or less, and the redox potential is -100 mV or less. This is the process.

The second fermentation step is a step of adding a ripening liquid of Stevia stalk to the microorganism-containing liquid that has undergone the first fermentation step and fermenting it until the pH becomes 3.1 or less to obtain a defective fermentation inhibitor. The amount of the stevia stem aging liquid added to the microorganism-containing liquid can be about 0.38 to 0.4 parts by weight with respect to 0.38 parts by weight of the microorganism-containing liquid.

Here, the ripening liquid of the Stevia stem used in the second fermentation step was obtained by immersing the Stevia stem in water or the like to extract the extract and fermenting it with a microorganism derived from the Stevia stem. A fermented liquid (hereinafter, also referred to as a simple ripening liquid for Stevia stems) can also be used.

However, the aging liquid of Stevia stalk is a Stevia stalk dispersion preparation step, a Stevia stalk dispersion container storage step, a Stevia stalk-derived microorganism selection step, a Stevia stalk extract preparation step, an extract concentration step, and a ripening step. It is more desirable to prepare through.

The Stevia stalk dispersion preparation step is a step of adding water to the dried Stevia stalk powder to prepare the Stevia stalk dispersion. Specifically, 10 parts by weight ± 10 parts by weight with respect to 1 part by weight of the dried Stevia stalk powder. Prepare by adding about 2 parts by weight of water.

The Stevia stalk dispersion liquid container accommodating step is substantially the same as the above-mentioned container accommodating step, and is accommodating in a container capable of forming a weakly sterilized region so that complete sterilization is not performed when heated in the Stevia stalk-derived microorganism selection step described later. It is a process.

The weakly sterilized region in the step of accommodating the stevia stalk dispersion liquid container is 10 cm or more from any position on the outer surface of the accommodation shape of the stevia stalk dispersion liquid contained in the predetermined container, and is on the outer surface. It is an internal area that is 17 cm or less from at least one of the positions. More preferably, a container is used in which a non-selected region exceeding 17 cm is not formed from any position on the outer surface and a weakly sterilized region can be formed.

The Stevia stem-derived microorganism selection step is also a step of heating the Stevia stem dispersion to select Stevia stem-derived microorganisms, similarly to the above-mentioned microorganism selection step. In detail, the first Stevia stem heating step and the second Stevia stem heating step It consists of a stevia stem heating step.

In the first step of heating the stevia stalk, a predetermined container containing the stevia stalk dispersion liquid is placed in the heating space through the step of accommodating the stevia stalk dispersion liquid container, and the heating space is kept at about 2.5 atm from the state of normal temperature and pressure. The temperature is raised to a state of 150 to 160 ° C over 30 to 40 minutes, and the state of 150 to 160 ° C is maintained at about 2.5 atm for 1 to 3 minutes.

In the second stevia stalk heating step, following the first stevia stalk heating step, the heating space is set to lower the temperature to 115 to 125 ° C. at about 2 atm for 3 to 5 minutes, and the temperature is set to 115 to 115 at about 2 atm. Maintain at 125 ° C for 20-40 minutes.

Then, after passing through the first stevia stalk heating step and the second stevia stalk heating step, by cooling to 85 to 95 ° C., the dried stevia stalks derived from the dried stevia stalks heat-selected in the stevia stalk dispersion. Leave the microorganisms behind.

The Stevia stem extract preparation step is a step of obtaining a liquid phase by solid-liquid separation from the Stevia stem dispersion which has undergone the Stevia stem-derived microorganism selection step.

Specifically, following the second Stevia stem heating step described above, the Stevia stem dispersion liquid, which has been cooled to 85 to 95 ° C., is solid-liquid separated by at least a means capable of transferring the microorganism to the liquid phase, and the liquid phase is obtained. Is obtained as an extract of Stevia stalk. This Stevia stalk extract has a Brix value of 2.0 to 4.0 and contains Stevia stalk-derived microorganisms.

The extract concentration step is a step of obtaining a stevia stem concentrate by heating and boiling the stevia stem extract to a predetermined concentration. Specifically, the stevia stalk extract is heated and boiled down, and concentrated so that the Brix value is 4.0 to 7.0, the pH is 6.0 or less, and the ORP is 10 to 99 mV in a state of being cooled to about room temperature.

The aging step is a step of aging the Stevia stalk concentrate at room temperature with fermentation to obtain a Stevia stalk ripening solution having a pH of 5.0 or less and an ORP of -100 mV or less.

As described above, the step of preparing the stevia stalk dispersion, the step of accommodating the stevia stalk dispersion container, the step of selecting the microbial stem-derived microorganisms, the step of preparing the stevia stalk extract, the step of concentrating the extract, and the aging step are performed. Therefore, a ripening liquid of Stevia stalk suitable for producing a defective fermentation inhibitor can be obtained.

Then, the liquid produced through the above-mentioned standing step, container storage step, microbial selection step, solid-liquid separation step, first fermentation step, and second fermentation step is subjected to bottom sediment improvement microbial fermentation. If it is used as a liquid, it is possible to more steadily improve the bottom sediment in which hedro and the like have accumulated.

Although this sediment-improving microbial fermented liquid is obtained by the above-mentioned method, this does not limit the method for producing the sediment-improving microbial fermented liquid, and the sediment-improving microbial fermented liquid is used. It is specified by the production methods of the standing step, the container containing step, the microorganism selection step, the solid-liquid separation step, the first fermentation step, and the second fermentation step.

In general, natural products such as plant powder, rice bran, okara, and brackish water contain various components and microorganisms in a complicated state and compounding ratio, like other natural products. Then, under the above-mentioned screening environment, predetermined microorganisms that can utilize components that could not be used by other microorganisms or that can find utility value in metabolites of certain microorganisms are selected, and further, components constituting these raw materials. It is considered that it can function as a bottom sediment-improving microbial fermentation liquid capable of suitable fermentation due to the presence of.

However, identifying the individual bacteria that make up the microbial flora that cause such bottom sediment improvement, quantifying the number of the bacteria, and defining the type and concentration of the components derived from each raw material is a manufacturing process. Considering the fermentation metabolism by microorganisms and their products in the above, it is impossible or almost impractical to directly identify them with predetermined parameters or the like.

Therefore, the bottom sediment-improving microbial fermentation broth was specified by the production method because the bottom sediment-improving microbial fermentation broth can be specified only by the production method. The same applies to the sediment-improving microbial fermented liquid as a component of the sediment-environment-improving material according to the present embodiment. However, this does not prevent the applicant from intentionally limiting the interpretation of a part or all of the method for producing the bottom sediment-improving microbial fermentation broth when acquiring the rights of the present application.

The bottom sediment-improving microbial fermented liquid impregnates dried water moss in a supersaturated state. Here, the supersaturated state means that the amount of the bottom sediment-improving microbial fermented liquid that can be impregnated and retained by the dried water moss is added in excess, and if it is lifted while being picked by hand, the bottom sediment-improving microbial fermented liquid will be released from the water moss. The amount is about to drip. In this way, the liquid-wet water moss can be prepared by adding the bottom sediment-improving microbial fermentation liquid to the dried water moss in a supersaturated state.

The steel slag is slag produced as a by-product in the manufacturing process of steel products, and is particularly preferably granulated with a size of about 3 to 10 mm.

The mixing ratio of the dried water moss and the granular steel slag is preferably 150 to 155 parts by weight of the granular steel slag with respect to 15 to 25 parts by weight of the dried water moss. If the ratio of steel slag to dried water moss is less than 150 parts by weight, there are problems that the adsorption force is lowered, the specific weight is insufficient, and the effect of improving the bottom sediment is poor. In addition, if the ratio of steel slag to dried water moss exceeds 155 parts by weight, iron peroxide may be formed, and the effect of improving the bottom sediment may be poor or the bottom sediment may be disturbed due to excessive supply of iron ions. There is a risk that it will end up.

The mesh-like bag that houses the wet water moss and granular steel slag is made of biodegradable resin. For example, when 3 to 5 months have passed at the bottom of the water, the liquid wet water moss and granular steel slag are removed. The strength is such that the function of assembling is lost. Such a mesh-shaped bag can be realized by, for example, a non-woven fabric having a thickness of about 0.1 mm formed of polylactic acid.

Then, the bottom sediment environment improving material according to the present embodiment is constructed by accommodating the liquid-wet water moss and the granular steel slag in such a mesh-shaped bag.

As described above, since the sediment environment improving material contains 150 to 155 parts by weight of granular steel slag with respect to 15 to 25 parts by weight of dried water moss, the sediment improving microbial fermentation added to the hypersaturated state. The growth of microorganisms in the liquid is suppressed by the elution component from steel slag, especially iron ions, and quality changes can be prevented during distribution and storage, and it is not relatively affected by storage time, etc. It is possible to start improving the environment of the sediment with a uniform number of initial bacteria.

In addition, the bottom sediment environment improvement material has a structure in which liquid-wet water moss and granular steel slag are housed in a mesh-like bag. It has a structure that can be thrown into a bag, and since it contains granular steel slag, it has a weight suitable for long-distance casting but has flexibility that allows it to be deformed. In this case as well, the worker has good handleability that the material for improving the sediment environment can be easily placed.

In addition, as described above, the liquid-wet water moss and the granular steel slag are put together in a mesh-shaped bag, and the granular steel slag spreads widely in the bottomed bag, and the sediment environment improving material is stably fixed to the bottom of the water. Therefore, it is possible to exert the effect that it is difficult to be washed away by the water flow.

In addition, since the mesh bag is made of biodegradable resin, the mesh bag spontaneously collapses due to microorganisms and the like after a predetermined period of time has passed after landing, and the wet water moss that was restrained by the bag. And granular steel slag naturally diffuses and dissipates due to water flow or the like.

That is, for a certain period of time after landing, liquid-wet water moss and granular steel slag are gathered and kept in place, so that a state in which sediment-improving microorganisms are present at a relatively high concentration appears in a timely manner. Therefore, during this period, the sediment-improving microorganisms are promoted to proliferate predominantly in comparison with the microorganisms existing in the sludge environment, and at the same time, the acclimatization of the sediment-improving microorganisms to the sludge environment progresses.

Then, the sediment-improving microorganisms that have been enriched and acclimated spread to the surrounding environment together with dried aquatic plants (liquid wet moss) along with the natural collapse of the mesh bag, and exert a sediment-improving effect over a wider area. It becomes. At the time of this diffusion, the bottom sediment improving microorganisms do not simply spread, but spread together with the aquatic plants that are the basis of the bottom sediment improving microorganisms, so that the settlement of the bottom sediment improving microorganisms at the diffused destination can be further promoted.

It should be noted that the introduction of the sediment environment improving material into water triggers the withdrawal of the sediment improving microorganisms from the suppressed state of reproduction and fermentation during distribution, and the sediment improving microorganisms start to grow and acclimatize as described above. That is, during distribution, iron ions derived from steel slag are present in high concentration through the bottom sediment improving microbial fermentation liquid impregnated in dry aquatic plants so that they drip, which suppresses the growth and fermentation of bottom sediment improving microorganisms, but in water. The addition causes dilution of iron ions, which triggers the start of growth and acclimatization of the sediment-improving microorganisms.

Hereinafter, the sediment environment improving material according to the present embodiment will be described in more detail with reference to specific manufacturing examples.

[1. Preparation of sediment-improving microbial fermented liquid]
(1-1) Screening for sediment-improving microorganisms
In a 20L capacity stainless steel container (32 cm in diameter and 38 cm in height), 0.85 kg of dried brackish stem powder, 0.85 kg of rice brackish powder, and 0.85 kg of dried brackish water powder are put into it, and then Abu River in Yamaguchi Prefecture. Prepare several batches of brackish water collected in the brackish water area of the river mouth (area with a salinity of about 0.8 to 1.0%), add 17 kg of steam water, stir and mix, and leave it at room temperature overnight (about 15 to 17 hours). (Standing process). Microbial examination of this stationary mixture confirmed the presence of at least Lactobacillus buchneri among several detected microorganisms.

In addition, a separate microbiological test was performed on the collected brackish water, and it was confirmed that the brackish water contained a group of salt-tolerant yeasts at a concentration of 150 cfu / ml.

Next, 20 L of the mixed solution that had undergone the static step was stored in a stainless steel container P1 with a lid (inner diameter 28 cm and height 33 cm) having a capacity of about 20.31 L, and a weakly sterilized region was formed in the container (container storage). Process). Similarly, in a stainless steel container Q1 with a lid having a capacity of about 21.04 L (cylindrical shape with an inner diameter of 20 cm and a height of 67 cm), 20.41 L of the mixed solution that has undergone the static process is stored, and the mixture is stored in the stainless steel container Q1. A weakly sterilized region was formed by forming the liquid storage shape into a cylinder with a diameter of 20 cm and a height of 65 cm. At the same time, 30.85 L of the mixed solution that has undergone the static process is stored in a stainless steel container R1 with a lid (inner diameter 34 cm and height 36 cm) having a capacity of about 32.67 L, and the mixed solution contained in the stainless steel container R1 is stored. A weakly sterilized region was formed with a cylindrical shape having a diameter of 34 cm and a height of 34 cm.

Next, the mixed solution is stored in the pressurized cooker together with each stainless steel container P1Q1 and R1, and the temperature is raised to 150 to 160 ° C. over 45 to 60 minutes at about 2.5 atm, and 150 to 150 at about 2.5 atm. The state of 160 ° C. was maintained for 1 to 3 minutes (first heating step).

Next, the temperature was subsequently lowered to a state of 115 to 125 ° C. at about 2 atm for 3 to 5 minutes, and the state of 115 to 125 ° C. was maintained at about 2 atm for 20 to 40 minutes (second heating step). ).

Next, the heating switch of the pressurized cooker was turned off, and the temperature was lowered over 24 to 30 hours until the internal temperature of the pressurized cooker became a state of normal temperature and pressure (temperature lowering step).

Then, the lid of the pressure cooker was opened to obtain a mixed solution in which the bottom sediment improving microorganisms were screened in each of the stainless steel containers P1, Q1 and R1. As a result of microbial examination of this mixed solution, the presence of at least Lactobacillus buchneri was confirmed among several detected microorganisms.

[2. Preparation of stevia stem ripening liquid]
First, 10 ± 2 kg of water was added to 1 kg of dried stevia stalk powder in a predetermined container and mixed evenly to prepare a stevia stalk dispersion (stevia stalk dispersion preparation step). Several batches of this Stevia stem dispersion were prepared.

Next, 12 L of Stevia stalk dispersion prepared in the Stevia stalk dispersion preparation step was housed in a stainless steel container P2 with a lid (inner diameter 25 cm and height 25 cm) having a capacity of about 12.27 L, and the Stevia stalk was housed in the container. A weakly sterilized region was formed in the dispersion liquid (stevia stem dispersion liquid container storage step). Similarly, a stainless steel container Q1 with a lid having a capacity of about 21.04 L (cylindrical shape with an inner diameter of 20 cm and a height of 67 cm) contains 12 L of the stevia stem dispersion prepared in the stevia stem dispersion preparation step, and the stainless container Q1 A weakly sterilized region was formed by forming a cylindrical shape of the Stevia stem dispersion liquid contained therein with a diameter of 20 cm and a height of 38 cm. At the same time, 30.85 L of Stevia stalk dispersion prepared in the Stevia stalk dispersion preparation step is stored in a stainless steel container R1 with a lid (inner diameter 34 cm and height 36 cm) having a capacity of about 32.67 L, and inside the stainless steel container R1. A weakly sterilized region was formed as a columnar shape with a diameter of 34 cm and a height of 34 cm.

Next, in the pressurized cooker, the stevia stalk dispersion liquid is stored together with the stainless steel containers P2, Q1 and R1, and the temperature is raised to 150 to 160 ° C over 30 to 40 minutes at about 2.5 atm, and the temperature is raised to about 2.5 atm. The temperature was maintained at 150 to 160 ° C. for 1 to 3 minutes (first stevia stalk heating step).

Next, the temperature was subsequently set to lower the temperature at about 2 atm for 3 to 5 minutes until the temperature was 115 to 125 ° C, and the temperature was maintained at about 2 atm at 115 to 125 ° C for 20 to 40 minutes (second stevia). Stevia heating process).

Next, the heating of the pressure cooker was completed, and the temperature inside the pressure cooker became approximately normal pressure (pressure that can be opened) and reached about 85 to 95 ° C. The containers P2, Q1 and R1 were taken out from the pressure cooker, and the stevia stalk dispersion was transferred into a cotton cloth bag.

The cloth bag containing the Stevia stalk dispersion was subjected to a dehydrator in a hot state and solid-liquid separated to obtain a Stevia stalk extract (Stevia stalk extract preparation step). This Stevia stalk extract has a Brix value of 2.0 to 4.0, and a microorganism of the genus Lactobacillus, which is a microorganism derived from Stevia stalk, is contained in all of the stainless steel containers P2, Q1 and R1 according to a separate microbiological test. Was confirmed.

Next, the obtained stevia stalk extract was placed in a heat-resistant container, placed on a gas stove, and boiled while heating for about 4 to 5 hours to prepare a stevia stalk concentrate (extraction). Liquid concentration step). After that, when heating was completed and the stevia stalk concentrate was allowed to cool to room temperature and then subjected to a physicochemical test, it was confirmed that the Brix value was 4.0 to 7.0, the pH was 6.0 or less, and the ORP was 10 to 99 mV. ..

Next, the obtained Stevia stalk concentrate was housed in a fermentation aging can (12 L capacity) with a stainless steel hook, and allowed to stand in a room temperature environment for aging (aging step). During aging, gas with a fermented odor is generated from any of the stevia stem concentrates of the stainless steel containers P2, Q1 and R1, and the fermentation is about the same regardless of the difference in the container shape of the stainless steel containers P2, Q1 and R1. It was confirmed that it was accompanied by.

Then, when the pH of the stevia stalk concentrate during aging was 5.0 or less and the ORP was -100 mV or less, the stevia stalk ripening solution was used. Further, at this time as well, no difference such as abnormal fermentation due to the container shape of the stainless steel containers P2, Q1 and R1 was observed, and the stevia stem aging liquid was treated as the same in the subsequent experiments.

[3. Preparation of sediment-improving microbial fermented liquid]
The above-mentioned [1. The mixed liquids in the stainless steel containers P1, Q1 and R1 that have been screened for the sediment-improving microorganisms through the temperature lowering step by [Screening for sediment-improving microorganisms] are separately housed in cotton cloth bags, and the cloth bags are dehydrated. The mixture was subjected to solid-liquid separation by using the apparatus to obtain a microorganism-containing liquid (solid-liquid separation step).

Next, in a fermentation and aging can with a hook (12 L capacity), about 9 kg of solid-liquid separated microbial-containing liquid was placed in separate stainless steel containers P1, Q1 and R1, and 0.1 kg of maple syrup was added as a sugar source and stirred uniformly. Then, it was allowed to stand at normal temperature and pressure for about 20 to 30 days for fermentation (first fermentation step).

In the initial stage of this first fermentation step, a supernatant is formed on the surface of the microbial-containing liquid, which, in the experience of the inventors, was removed every few days to slow down the fermentation. In addition, after this initial stage, the microbial-containing liquid began to foam as if it were beer, and it was confirmed that fermentation was carried out by the microorganisms.

Then, the first fermentation step was completed when the pH of the microorganism-containing liquid was 4.5 or less and the redox potential was -100 mV or less. At this time, no difference such as abnormal fermentation due to the difference in container shape among the stainless steel containers P1, Q1 and R1 was confirmed.

Next, with respect to the microorganism-containing liquid that has undergone the first fermentation step, the above-mentioned [2. Preparation of Stevia stalk aging solution] The Stevia stalk aging solution obtained was added, stirred uniformly, and allowed to stand at normal temperature and pressure for about 20 to 25 days for fermentation (second fermentation step). ).

Specifically, a 0.75 kg stainless steel container P1, Q1, or R1 microbial-containing solution that has undergone the first fermentation step and 0.75 kg of stevia stalk aging solution are stored in a 10 L capacity secondary fermentation can. Further, water was added to make 10 kg, and the mixture was uniformly stirred to prepare a liquid to be fermented for the second fermentation step. If necessary, a sugar source of about 0.75 kg may be further added to the fermented liquid, and for example, oligosaccharides, more preferably sugar beet sugars can be added.

Then, when the fermentation was performed until the pH became 3.1 or less, the second fermentation step was completed, and the obtained fermentation broth was used as a sediment-improving microbial fermentation broth. In addition, at this point as well, no difference such as abnormal fermentation due to the container shape of the stainless steel containers P1, Q1 and R1 was observed, and in the subsequent tests, the bottom sediment-improving microbial fermentation liquids were all treated as the same. did. The number of photosynthetic bacteria belonging to the genus Chromatium in the fermented microbial solution for improving sediment was 20 cfu / ml or less. Furthermore, cell counts salt tolerance yeast group was 1,2 × 10 ⁶ cfu / ml.

[4. Steel slag]
Steel slag was obtained from a steel company and used as a raw material for manufacturing the sediment environment improvement material according to this embodiment. The steel slag was granular with a diameter of about 3 to 10 mm.

[5. Sphagnum]
A commercially available dried moss was obtained from a home center and used as a raw material for producing the sediment environment improving material according to the present embodiment.

[6. Mesh bag]
A polylactic acid non-woven fabric (model number 9051-89-2) manufactured by Shinwa Co., Ltd. was obtained, and a 25 cm × 25 cm rectangular bag with an opening on one side was formed using a heat sealer to form a mesh bag. The thickness of the polylactic acid non-woven fabric was about 0.1 mm, and the opening was large enough to prevent steel slag and water moss from falling off (approximately 1 mm or less).

[7. Sediment environment improvement material]
Next, a sediment environment improving material was formed using the above-mentioned sediment-improving microbial fermented liquid, steel slag, water moss, and mesh bag.

First, 15 to 25 g of dried water moss and 150 to 155 g of granular steel slag were placed in a mesh bag, and the opening was sealed with a heat sealer to form a mesh bag container.

Next, the obtained mesh bag container was immersed in the bottom sediment improving microbial fermented liquid to impregnate the dried water moss in the mesh bag containing body with the bottom sediment improving microbial fermented liquid to obtain a liquid wet water moss.

After 12 to 24 hours had passed, it was pulled up from the bottom sediment improvement microbial fermentation broth to obtain a bottom sediment environment improvement material. At this time, it was confirmed that the dried water moss had sufficiently completed the absorption of the bottom sediment-improving microbial fermentation liquid and was impregnated in a supersaturated state.

Three bags of the obtained sediment environment improving material are housed in a polyethylene-based polyamide composite resin outer bag, and a predetermined amount of the bottom sediment improving microbial fermented liquid is stored to seal the opening in order to maintain a supersaturated state. Then, it was used as a packaging material for improving the sediment environment. The amount of the sediment-improving microbial fermented liquid to be contained is not particularly limited in the range of, for example, about 200 to 2000 ml, but if the weight of the sediment environment-improving material package is to be reduced as much as possible, 400 to 600 ml is used. It can be in the range of 1300 to 1700 ml if there is another use for the excess sediment-improving microbial fermented liquid contained in the packaging of the sediment environment-improving material.

[8. Sediment environment improvement test (1)]
Next, using the manufactured sediment environment improvement material, a field test was conducted to confirm the effect of improving the sediment environment.

Specifically, Company Y, which operates a fishery wholesale business in Sasebo City, Nagasaki Prefecture, attempted to improve the bottom sediment of the sea, which is located under the floor of the floating pier used as a loading site. On the seabed directly under the loading site, a large amount of organic matter is accumulated due to the landing of seafood and the loading and unloading of fishery materials, and the sediment is deteriorated. According to this, the pH was 6.34, the ORP was -261 mV, and the hydrogen sulfide concentration was 2.0 ppm.

In order to improve the bottom sediment in this target area, in this test, the target area was divided into the following four areas, and the effects were confirmed under different conditions.

First, in the first section, a total of 12 sediment environmental materials according to the present embodiment, which can be said to be almost immediately after production and within 3 days of production, are approximately ^{1 in 30 m 2 (bottom).} 4 bags) were placed as a packaging material for improving the quality and environment.

Further, in order to compare with the first section, the second section contains the bottom sediment environmental material according to the present embodiment, which is stored in the warehouse for 12 months after production (hereinafter, also referred to as long-term storage material). refers.) was roughly a total of 12 in a ratio of one to 30 m ² (4 bags as long-term storage have been sediment environmental improvement materials packaging) arranged to separate the partitioned area of 360 m ² it was.

Further, as a further comparison area, the third section is a material which is substantially the same as the sediment environment improvement material according to the present embodiment but excluding only granular steel slag, and can be said to be manufactured almost immediately after production. 3 days within one (hereinafter, also referred to as slag no comparative.) a generally total of twelve in a ratio of one to 30 m ² for a region of 360 m ² which is partitioned separately (sediment environmental improvement materials like packaging As 4 bags) were placed.

As a further comparison target, in the fourth section, a slag-free comparative product (hereinafter, also referred to as a long-term storage slag-free comparative product) stored in the warehouse for 12 months after production is separately divided into 360 m ² A total of 12 pieces (4 bags as a packaging material for sediment environment improvement materials) were placed at a ratio of about 1 piece per ^{30 m 2} to the area of.

For the materials and comparative products placed in each section, poles were placed as markers so that the initial positions could be known.

First, when the workability of each material and comparative product during the placement work was confirmed, in the first and second sections, due to the moderate weight of the granular steel slag mixed in the material, it was thrown to the target position. It was confirmed that the work could be performed and the workability was extremely excellent. On the other hand, in the third section and the fourth section, granular steel slag is not blended in the comparative products to be arranged, and the mesh bag lacks a high-density weight feeling like metal only with liquid-wet water moss. Although it is not impossible to arrange by throwing, it is difficult to control the target position, and the workability is lacking as compared with the sediment environment improvement material according to the present embodiment used in the first section and the second section. ..

Next, the changes over time after the placement work of each material and comparative product were examined. The evaluation was performed on the state of the mesh bag, the amount of movement of the mesh bag from the initial placement position, pH, ORP, and hydrogen sulfide concentration. First, Table 1 shows the results of the first section and the second section.

First, regarding the first section, as can be seen from Table 1, the state of the mesh bag showed almost no change until one month after placement, but it was a fragile part that seemed to be slightly unraveled in the second month. Has come to be seen. Although not shown in Table 1, the state of the mesh bag gradually unraveled, and a part of the mesh bag collapsed in the 4th month, and the 12 bottoms arranged in the 5th to 6th months. Most of the materials for improving the quality and environment had already been washed away by the ocean current along with the bags. From this, it was confirmed that the state of the mesh bag changes in a timely manner.

Regarding the movement of the mesh bag from the initial placement position, it was within 1 meter from the pole, which is the marker of the initial position, until the second month after the placement. Moreover, when the progress after that was observed, it was within the range of 2 meters even after 4 to 6 months had passed. From this, it was suggested that the sediment environment improving material according to the present embodiment is relatively difficult to be washed away by the water flow.

In addition, the pH of the sample water was 6.34 immediately after placement as described above, and this value was 6.35 without much change even after one month. However, after two months, the value changed significantly to 5.93.

The ORP value of the sample water was -261 mV immediately after placement, but the potential increased slightly to -201 mV after 1 month and further increased to -185 mV after 2 months. In the subsequent follow-up, the ORP value finally reached 128 mV after 6 months.

The hydrogen sulfide concentration in the sample water was 2.0 ppm immediately after placement, but decreased significantly to 0.2 ppm after 1 month and fell below the detection limit after 2 months. In the subsequent follow-up, hydrogen sulfide was not detected even after 6 months.

From the results of these first compartments, it is promoted that the sediment-improving microorganisms proliferate predominantly in comparison with the microorganisms existing in the sludge environment for a certain period after landing in which the mesh bag is maintained, and at the same time, the sediment The acclimatization of the improving microorganisms to the sludge environment progressed, and then the sediment-improving microorganisms that were enriched and acclimatized spread to the surrounding environment together with the dried aquatic plants (liquid wet water moss) with the natural collapse of the mesh bag, and spread more widely. It was suggested that the effect of improving the sediment was exhibited over the years.

In contrast to the results of the first section, as shown in the substantially right half of Table 1, in the case of the second section using long-term storage materials, the ORP value and hydrogen sulfide concentration after one month are the first section. Although it was slightly inferior to the result in, after 2 months, the result was almost the same as that in the first section.

It is thought that this is because the instantaneous power of microbial growth immediately after the start of the test decreased slightly due to long-term storage, but the decrease in the instantaneous power was also slight, and it was considered that the material was not stored for a long time after 2 months. The same effect could be produced. That is, the result of the second section was almost the same as the result of the first section, and it was considered that the influence of the storage period of 12 months on the quality of the product was small.

Next, the results of the third and fourth sections are shown in Table 2.

Regarding the results of the third section using the slag-free comparative product, it is in the state of a mesh bag as shown in Table 2, but when I visited the test site one month later, the slag-free comparative product placed in the third section Were all lost due to the tide. Therefore, we placed a slag-free comparison product at the position of the pole marker again and fixed it with a weight to prevent it from being lost.

As a result, although no change was confirmed at 2 months (1 month after relocation), a solution appeared at 3 months (2 months after relocation) in the subsequent follow-up, and 4 after relocation. A part of the mesh bag collapsed in the month, and most of the 12 sediment environment improving materials placed 5 to 6 months after the rearrangement had already been washed away by the ocean current. From this, it was confirmed that the timed change in the state of the mesh bag is the same as that of the bottom sediment environmental material or the long-term storage material according to the present embodiment.

Further, the movement of the mesh bag from the initial arrangement position is as described above, and since it does not have granular steel slag, the material itself seems to be easily washed away.

In addition, the pH of the sample water was 6.33 immediately after placement as described above, and since the material itself was lost, this value did not change much even after one month and was 6.35. In addition, although the value changed to 6.11 after 2 months, the degree of the change was smaller than that in the first and second compartments.

The ORP value of the sample water was -263 mV immediately after placement, but after 1 month the potential rose slightly to -248 mV despite the loss of the material itself, and after 2 months it was slightly -222 mV. Continued to rise little by little. This is because materials containing highly active microorganisms, which are difficult to settle but are shortly after production, are used, so even after the materials are lost due to ocean currents, they settle on the seabed and gradually settle in the sediment environment. It is probable that this was due to improvements. However, it was considered that the reason why the bottom sediment environment did not improve dramatically even after fixing with a weight was that there was no supply of iron ions from the steel slag.

The hydrogen sulfide concentration in the sample water was 2.1 ppm immediately after placement, 1.9 ppm after 1 month, and 1.6 ppm after 2 months. Similar to the ORP value, this hydrogen sulfide concentration also improved the sediment environment slightly by colonizing the bottom soil of microorganisms, but due to the absence of steel slag (insufficient iron ions), hydrogen sulfide was dramatically improved. It is probable that no reduction was seen.

From these facts, it was shown that the existence of steel slag is useful for making a dramatic improvement in the sediment environment.

In contrast to the results of the third section, as shown in the substantially right half of Table 2, in the fourth section using the long-term storage slag-free comparative product, the state of the mesh bag and the movement from the initial placement position Although the points were the same, the result was even inferior to the result of the third section in terms of the effect of improving the sediment environment.

That is, it was observed that the pH value continued to decrease slightly from 6.33 (immediately after) → 6.28 (1 month later) → 6.25 (2 months later), but for ORP, the value was -255 mV immediately after placement. It was -262 mV after 1 month and -248 mV after 2 months, and almost no improvement tendency was observed.

In addition, the hydrogen sulfide concentration did not show a remarkable improvement tendency from 2.1 ppm (immediately after) → 2.0 ppm (1 month later) → 1.9 ppm (2 months later).

Taking these results into consideration, the long-term storage slag-free comparative product has the effect of improving the bottom sediment due to the absence of steel slag, which is the source of iron ions, and the markedly inferior fixation to the bottom soil due to the decrease in microbial activity due to long-term storage. Was considered to have been rarely seen.

[9. Sediment environment improvement test (2)]
Next, a second field test was conducted to confirm the effect of improving the environment of the sediment using the manufactured material for improving the environment of the sediment.

Company O, a fishery seedling company, tried to improve the bottom sediment in the tidal flats near the drainage outlet in front of the company building. According to the sample water collected from the bottom soil of the target area, the pH of the tidal flat before the environmental improvement test was 6.22, the ORP was -151 mV, and the hydrogen sulfide concentration was below the detection limit (0.1 ppm or less).

The test was carried out by dividing the target area into the first to fourth sections as in the above-mentioned sediment environment improvement test (1).

As a result, the same tendency as in the above-mentioned test was observed in the state of the mesh bag and the movement from the initial arrangement position in the first section. The pH was 6.22 (immediately after) → 6.42 (1 month later) → 5.85 (2 months later), and the pH tended to decrease between immediately after the start of the test and 2 months later, but 1 month. The reason for the rise once later is currently being clarified.

In addition, the ORP value steadily increased from -151 mV (immediately after) → 41 mV (1 month later) → 101 mV (2 months later). Of particular note was the improvement of the sediment environment to a potential exceeding 100 mV after 2 months. The hydrogen sulfide concentration was below the detection limit from the beginning, and this continued until the end of the test.

On the other hand, for the other plots, the same results as in the above-mentioned sediment environment improvement test (1) were obtained. That is, it was confirmed that the second section has an effect of improving the sediment environment, which is almost the same as that of the first section. In addition, although a slight effect of improving the sediment environment was observed in the third section, the effect was significantly lower than that of the first and second sections. Further, the effect of improving the sediment environment was lower in the fourth section than in the third section.

From these results, the sediment environment improving material according to the present embodiment is less likely to change in quality during distribution and storage, is excellent in handling, is not easily washed away by water flow, and has a relatively high amount of useful bacteria. It was suggested that it is possible to make the site gathered at the concentration appear at the bottom in a timely manner and then diffuse to the bottom to bring about a wide range of improvement effects.

As described above, according to the bottom sediment environment improving material according to the present embodiment, 15 to 25 parts by weight of dry water moss impregnated with the bottom sediment improving microbial fermented liquid in a hypersaturated state, and 150 ~ 155 parts by weight of granular steel slag and granulated steel slag are housed in a mesh-like bag made of biodegradable resin, so the quality does not change easily during distribution and storage, and it is excellent in handling. In addition, it is difficult to be washed away by water flow, and a site where useful bacteria are gathered at a relatively high concentration appears at the bottom in a timely manner, and then diffuses to the bottom to bring about a wide range of improvement effects. Improvement materials can be provided.

Finally, the description of each embodiment described above is an example of the present invention, and the present invention is not limited to the above-described embodiment. Therefore, it goes without saying that various changes can be made according to the design and the like as long as the technical idea of the present invention is not deviated from the above-described embodiments.

Claims (2)

A liquid-wet water moss obtained by impregnating 15 to 25 parts by weight of a dry water moss with a bottom sediment-improving microbial fermented liquid in a hypersaturated state and 150 to 155 parts by weight of granular steel slag were formed of a biodegradable resin. A material for improving the bottom sediment environment that is housed in a mesh-shaped bag. The sediment-improving microbial fermented liquid is
A mixture of 0.6 parts by weight of dried stevia stalk powder, 0.6 parts by weight of rice bran powder, 0.6 parts by weight of dried okara powder, and 11 to 13 parts by weight of brackish water containing at least salt-tolerant yeast was prepared. A standing process that allows the product to stand for a predetermined period of time,
The mixed solution that has undergone the standing step is contained in a predetermined container, and the contained mixed solution is 10 cm or more from any position on the outer surface of the containing shape of the mixed solution, and at least on the outer surface. A container storage process that forms a weakly sterilized area that is 17 cm or less from any position,
A predetermined container containing the mixed solution is placed in the heating space through the container storage step, and the heating space is heated from a normal temperature and pressure state to a state of 150 to 160 ° C. at about 2.5 atm for 45 to 60 minutes. The temperature is raised, maintained at about 2.5 atm at 150 to 160 ° C for 1 to 3 minutes, and then the heating space is set to a state of 115 to 125 ° C at about 2 atm over 3 to 5 minutes, and the temperature is set to decrease to about 2. The state of 115 to 125 ° C. at atmospheric pressure is maintained for 20 to 40 minutes, and the heating space is further lowered to a state of normal temperature and pressure for 24 to 30 hours to leave the heat-selected microorganisms in the mixed solution. Microbial selection process and
A solid-liquid separation step of obtaining a microorganism-containing liquid by solid-liquid separation of the mixture that has undergone the microorganism selection step by at least a means capable of transferring the microorganism to the liquid phase.
A first fermentation step in which a sugar source is added to the obtained microbial-containing liquid and fermented at normal temperature and pressure for a predetermined time to set the pH of the microbial-containing liquid to 4.5 or less and the redox potential to -100 mV or less.
Fermentation obtained by adding a ripening solution of Stevia stalk to a microbial-containing liquid that has undergone the first fermentation step and fermenting it until the pH becomes 3.1 or less to obtain a second fermentation step as a defective fermentation inhibitor. The sediment environment improving material according to claim 1, which is a liquid.