JP2020105246A - Biofilm formation preventive agent for preventing biofilm formation of after cleaning, cleaning composition containing said agent, and method for preventing biofilm formation of after cleaning - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a biofilm formation preventing action after sufficient cleaning, unlike a bactericidal agent such as hypochlorite conventionally used for removing a biofilm, and to be safe for the human body and have an environmental load. It is convenient because it provides a biofilm formation inhibitor after cleaning, and it can prevent biofilm formation after cleaning and cleaning at the same time. It is an object of the present invention to provide a cleaning composition containing a biofilm formation inhibitor after cleaning, which can be prevented in advance. SOLUTION: The biofilm formation inhibitor for preventing the formation of a biofilm after washing, which contains a sophorose lipid, contains the biofilm formation inhibitor, and the above-mentioned biofilm formation inhibitor. The solution is a cleaning composition. [Selection diagram] None

Description

The present invention relates to a biofilm formation inhibitor for preventing biofilm formation after washing, a cleaning composition containing the agent, and a method for preventing biofilm formation after washing.

A biofilm is a structure formed by collecting microorganisms such as Pseudomonas aeruginosa and products such as extracellular polysaccharides produced by the microorganisms on the surface of a solid. In a biofilm, microorganisms are embedded in an extracellular matrix composed of products such as extracellular polysaccharides produced by themselves. Biofilms can cause microbial harm in a variety of locations, as they can occur in any location where water is present and microorganisms are prone to growth. For example, at home, a biofilm is formed around water in kitchen sinks, kitchens, washbasins, washing tubs, bathrooms, toilets, drains, drains, etc., causing slime and malodor, and in severe cases, It may cause clogging of drains and drains. In addition, if a biofilm is formed on things used around water such as tableware, cooking utensils, and washing tools, hygiene is impaired, which causes microbial contamination such as food poisoning. In factory plants that use water, the biofilms formed cause microbial contamination of products and deterioration of equipment due to metal corrosion. In hospitals, biofilms formed on medical devices cause nosocomial infections.

In order to remove the biofilm causing the damage by such microorganisms, a bactericide such as hypochlorite has been conventionally used, but the effect was insufficient. The reason is that, as described above, in the biofilm, the microorganism is embedded in the extracellular matrix made of a product such as extracellular polysaccharide produced by itself and protected from the bactericide. Further, hypochlorite is not safe for the human body because it is necessary to take care not to get it on the skin and mucous membranes, and the burden on the environment is high.

For sanitary use of household water, those used around water, and plant plants that use water, in addition to the removal of the biofilm described above, cleaning is required. The disinfectant such as chlorite and the cleaning agent are separately used. However, performing both separately is not only time-consuming and inconvenient, but also causes forgetting of maintenance and causes microbial damage due to the biofilm. Therefore, it is convenient if both can be performed at the same time, and at the same time, forgetting of care can be prevented and microbial damage due to biofilm can be prevented. In particular, it is possible to prevent the formation of biofilm after cleaning with a cleaning agent that is used for daily cleaning in household water such as tableware, cooking utensils, cleaning tools, etc. It is very convenient because it does not need to be removed, at the same time it can prevent forgetting to care, prevent slime and bad smell around water, clogging of drains and drain pipes, and after cleaning things used around water. Can keep the hygiene of. However, at present, the idea of simultaneously performing biofilm formation prevention and cleaning after cleaning is not found.

Sophorose lipid is known as a detergent that can be safely used by the human body and is excellent in biodegradability and has a low environmental load (Patent Documents 1 and 2). However, it is not known that sophorose lipid has an action of preventing biofilm formation after washing.

JP-A-2003-13093 JP, 2017-137242, A

The present invention, unlike bactericides such as hypochlorite, which are conventionally used to remove biofilms, has a biofilm formation preventing effect after sufficient washing, and is safe for humans and has a low environmental load. It is intended to provide a biofilm formation inhibitor after washing.

Further, the present invention, since it is possible to perform the biofilm formation prevention and washing after washing at the same time, it is convenient and at the same time, prevent forgetting of care, and can prevent microbial damage due to biofilm in advance. It is an object of the present invention to provide a cleaning composition containing a film formation inhibitor.

The inventors of the present invention have searched for various substances that have sufficient biofilm formation-preventing action after washing, are safe for human bodies, and have low environmental load, and have found that sophorose lipid satisfies all of these requirements. Further, since the sophorose lipid also has a cleaning action, it was found that the biofilm formation after cleaning and the cleaning can be carried out at the same time.

That is, the present invention provides a biofilm formation inhibitor for preventing biofilm formation after washing, which contains sophorose lipid.

According to a preferred embodiment of the present invention, there is provided the biofilm formation inhibitor, wherein 20% by weight or more and 80% by weight or less of the sophorose lipid is a lactone type sophorose lipid.

The present invention also provides a cleaning composition for preventing biofilm formation after cleaning, the cleaning composition containing the biofilm formation inhibitor.

According to a preferred embodiment of the present invention, there is provided the above-mentioned cleaning composition, wherein the content of the above-mentioned sophorose lipid is 0.05 to 10.0% by weight.

Furthermore, the present invention provides a method of preventing biofilm formation after washing, comprising the step of washing using the cleaning composition containing the SOHO loin lipid, intended to provide a biofilm formation preventing method is there.

According to a preferred embodiment of the present invention, the biofilm formation preventing method, wherein 20% by weight or more and 80% by weight or less of the sophorose lipid is a lactone type sophorose lipid; and the content of the sophorose lipid is A method for preventing biofilm formation is provided, which is 0.05 to 10.0% by weight.

The biofilm formation inhibitor after washing of the present invention can sufficiently prevent the formation of biofilm after washing, and is safe for human body and low in environmental load.

In addition, the biofilm formation inhibitor after cleaning of the present invention also has a cleaning action, and when contained in a cleaning composition, biofilm formation prevention and cleaning after cleaning can be performed at the same time, which is convenient. At the same time, care forgetting can be prevented, and microbial damage due to biofilm can be prevented.

In particular, when it is contained in a cleaning composition for daily cleaning around household water such as tableware, cooking utensils, and cleaning tools, it is possible to prevent biofilm formation after cleaning and cleaning at the same time. It is very convenient because it eliminates the need to remove biofilms used around water and water, and at the same time prevents forgetting of care and prevents slime and foul odor around water, clogging of drains and drains. At the same time, it is possible to keep the sanitary condition after cleaning the product used around water.

In the present embodiment, sophorose lipid has an effect of preventing biofilm formation after washing. In addition, it is safe for the human body and has a low environmental impact. Furthermore, since it has a cleaning action, it is suitably blended in a cleaning composition containing a biofilm formation inhibitor after cleaning.

The structure of sophorose lipid is shown below. Sophorose lipid is a glycolipid in which glucose dimer sophorose and hydroxy fatty acid are bound. Sophorose lipid is a mixture of an acid form in which a carboxyl group of hydroxy fatty acid is released and a lactone form in which this carboxy group is bonded to the hydroxy group of sophorose in the molecule. Part of the hydroxy groups of sophorose may be acetylated.

In the present embodiment, preferably 20% by weight or more and 80% by weight or less of the sophorose lipid is a lactone type sophorose lipid. More preferably, 40% by weight or more and 80% by weight or less of sophorose lipid, and even more preferably 60% by weight or more and 80% by weight or less of sophorose lipid are lactone type sophorose lipids. When the lactone type sophorose lipid is within the above range, not only the effect of preventing biofilm formation after washing is improved, but also the stability of the liquid property is increased, and turbidity or separation is less likely to occur, which is preferable.

Biofilm formation inhibitor after washing according to the present embodiment, and the content of sophorose lipid in the cleaning composition containing a biofilm formation inhibitor after washing, biofilm formation preventing action after washing, and washing From the viewpoint of strength, the content is preferably 0.05 to 10.0% by weight, and more preferably 0.05 to 5% by weight.

The biofilm formation inhibitor after washing according to the present embodiment, and the biofilm formation inhibitor-containing cleaning composition after washing, as a component other than sophorose lipid, a biofilm formation preventing action after washing of the present invention As long as it does not interfere with the cleaning action, it may contain a biofilm formation inhibitor after cleaning and components generally contained in cleaning compositions.

As a component that may be contained in the biofilm formation inhibitor-containing cleaning composition after cleaning, a component generally contained in the cleaning composition, for example, an alkyl sulfate-based surfactant, a betaine-based surfactant, an alkyl Surfactants such as ether sulfate ester-based surfactants, alkylamine oxide-based surfactants, fatty acid salt-based surfactants, etc., solubilizing agents such as ethanol, viscosity adjusting agents, pH adjusting agents, sterilizing agents, preservatives Examples include agents, fragrances, solvents such as water, and the like.

The content of each component is 0.1 to 5.0% by weight for alkyl sulfate-based surfactants, betaine-based surfactants, alkyl ether sulfate ester-based surfactants, and alkyl amine oxide-based surfactants. 5.0 to 20.0% by weight, solubilizer such as ethanol, viscosity adjusting agent 1.0 to 5.0% by weight, pH adjusting agent 0.5 to 1.0% by weight, fatty acid salt-based surfactant The amount of the agent, the disinfectant, the preservative, and the fragrance is preferably 0.5% by weight or less, and the balance is a solvent such as water.

The biofilm formation inhibitor after washing according to the present embodiment can be used to prevent the formation of biofilm that occurs in a place where any washing is necessary, but is preferably a kitchen sink, a kitchen, a wash basin, a washing tub. , A bathroom, a toilet bowl, a drain, a drain pipe, and other household water sources, and tableware, cooking utensils, cleaning tools, and other household water components that can be used to prevent the formation of biofilms.

A cleaning composition containing a biofilm formation inhibitor after cleaning according to the present embodiment can be used in any scene where biofilm formation prevention and cleaning after cleaning are required, but preferably a kitchen sink, After washing the kitchen, wash basin, washing tub, bathroom, toilet, drainage, drain pipe, etc. used around the home, tableware, cooking utensils, cleaning tools, etc. It can be used for biofilm formation prevention and cleaning. According to such an embodiment, it is possible to prevent biofilm formation after cleaning and cleaning at the same time, which is convenient, and at the same time, it is possible to prevent forgetting of care and prevent microbial damage due to biofilm.

Cleaning composition containing a biofilm formation inhibitor after cleaning according to the present embodiment, more preferably, when used for things that are washed daily around household water, such as tableware, cooking utensils, cleaning tools, Preventing biofilm formation after cleaning and cleaning can be performed at the same time, eliminating the need to separately remove biofilm with or without a sterilizing agent, which is very convenient and prevents forgetting of care. It is possible to prevent slimy and bad odor around water, clogging of drains and drain pipes, and also to maintain hygiene of products used around water after cleaning.

The method of using the cleaning composition containing the biofilm formation inhibitor after cleaning according to the present embodiment is the same as that of a general cleaning composition, and can be used for hand washing, dishwashing with a dishwasher, and the like. After washing, air drying, wiping with water, dry wiping and the like may be performed as usual.

1. Production Example (1) Preparation of Dishwashing Detergent A sophorose lipid-containing dishwashing detergent (Example 1) was prepared according to the formulation shown in Table 1 below. In addition, as a comparative example, a dishwashing detergent not containing sophorose lipid (Comparative Example 1) was prepared. In addition, Tables 2 to 4 show the ingredients of various commercially available dishwashing detergents (Comparative Examples 2 to 4) which are said to have a sterilizing effect.

2. Confirmation test of biofilm formation preventing action after washing (1) Outline of test Sophorose lipid-containing dishwashing detergent (Example 1), sophorose lipid-unblended dishwashing detergent (Comparative Example 1), and various commercially available detergents ( The preventive action against the Pseudomonas aeruginosa biofilm (BF) of Comparative Examples 2 to 4) was verified by evaluation by the CV staining method using a 96-well plate.

(2) Test bacteria
Pseudomonas aeruginosa NBRC 13275 (Pseudomonas aeruginosa)

(3) Test method
(3-1) Confirmation test of BF formation on the surface of the test product soaked
(a) Surface treatment of microplate with test product The test product was prepared to a specified concentration using sterile purified water, and 300 μl of each test product was added to the 96-well microplate column (8 holes). After allowing this to stand at room temperature for 3 hours, the test product was collected by a suction device, and further washed by adding sterile purified water and suctioning, and this was repeated 3 times. The surface-treated microplate was dried at room temperature.

(b) BF formation on surface-treated microplate The test strain was reconstituted from a cryopreserved product, and after pre-incubation with SCD agar for 24 hours, it was adjusted to 10 ⁶ to 10 ⁷ cfu/mL with sterile saline. Prepared. This was diluted with an original concentration of tryptosoy broth (TSB) so that the order was 1.0×10 ⁶ cfu/mL, and used as a bacterial-added TSB. 200 μl of this was added to each well of the surface-treated microplate, and the lid was placed on the surface of the plate, followed by incubation in a thermostat at 35° C. for 24 hours to form BF.

(c) Staining and extraction of BF by CV staining method
The BF-forming plate was washed from the wells by collecting the suspension of culture medium and proliferating bacteria from each hole with a suction device, adding sterile purified water to suction, and repeating this twice, then thoroughly drying at room temperature. It was On the dried plate, 220 μl of 0.1% (w/v) crystal violet (CV) aqueous solution was added to each well to stain the formed BF. After leaving still for 15 minutes, remove by aspiration, wash twice with sterile purified water and dry thoroughly, then add 250 μl of 99.5% ethanol to extract the residual CV and transfer it to a new microplate from each hole. 200 μl of each extract was collected and subjected to measurement by a microplate reader.

(4) Test results
(4-1) Confirmation test of BF formation on the surface of the test product immersion treatment Table 5 and Table 6 show the results of the CV staining test of BF formed on the 96-well microplate surface-treated by immersing the test product.

Regarding the BF formation prevention effect, the BF formation prevention rate (%) = {1-(average absorbance of sample/average absorbance of sterilized purified water) )}×100 was calculated and evaluated.

The dishwashing detergent containing sophorose lipid (Example 1) exhibited a biofilm formation-preventing action after washing, while the dishwashing detergent containing no sophorose lipid (Comparative Example 1) exhibited a biofilm-forming inhibiting action after washing. Since it did not show, it was confirmed that sophorose lipid has a biofilm formation-preventing action after washing. It should be noted that the commercial dishwashing detergents (Comparative Examples 2 to 4), which are said to have a sterilizing effect, did not show a biofilm formation preventing action after washing.

Claims (7)

A biofilm formation inhibitor for preventing biofilm formation after washing,
A biofilm formation inhibitor containing sophorose lipid. The biofilm formation inhibitor according to claim 1, wherein 20% by weight or more and 80% by weight or less of the sophorose lipid is a lactone type sophorose lipid. A cleaning composition for preventing biofilm formation after cleaning,
A cleaning composition comprising the biofilm formation inhibitor according to claim 1 or 2. The cleaning composition according to claim 3, wherein the content of the sophorose lipid is 0.05 to 10.0% by weight. A method for preventing biofilm formation after washing, comprising:
A method for preventing biofilm formation, comprising a step of washing with a washing composition containing sophorose lipid. The biofilm formation preventing method according to claim 5, wherein 20% by weight or more and 80% by weight or less of the sophorose lipid is a lactone type sophorose lipid. The biofilm formation preventing method according to claim 5 or 6, wherein the content of the sophorose lipid is 0.05 to 10.0% by weight.