JP2020012661A - Sample substrate - Google Patents

Abstract

To provide a sample substrate with a simple configuration used in a potential difference detecting device.SOLUTION: A sample substrate 10 used for a potential difference detecting device, includes: a first electrode 12 for measuring a first potential difference for a sample; a second electrode 11 for measuring the first potential difference; a cover 4 in which a sample injection hole 41 is located directly above the first electrode 12; and a sample absorber 3 for absorbing the sample when the sample is injected from the injection hole 41 and for bringing the first electrode 12 and the second electrode 11 into contact with the sample.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a sample substrate used in a potential difference detection device that detects a potential difference between samples.

  In general, in order to inspect a liquid such as blood, it is known to measure a potential difference generated by an oxidation-reduction potential (ORP, Oxidation Reduction Potential) or the like (for example, see Patent Documents 1 and 2).

Japanese Patent No. 5834319 Japanese Patent No. 5985739

However, once used, the sample substrate into which the liquid, which is the sample, is injected is often thrown away because it is difficult to wash the sample. Therefore, the sample substrate is required to have a simple configuration.
An object of an embodiment of the present invention is to provide a sample substrate having a simple configuration used in a potential difference detecting device.

  A sample substrate according to an aspect of the present invention is a sample substrate used for a potential difference detection device, and includes a first electrode for measuring a first potential difference with respect to a sample, and a first electrode for measuring the first potential difference. A second electrode, a cover in which the injection hole for the sample is located directly above the first electrode, and when the sample is injected from the injection hole, absorbs the sample and absorbs the sample. And a sample absorber for bringing the sample into contact with the second electrode.

  According to the embodiment of the present invention, it is possible to provide a sample substrate having a simple configuration used in a potential difference detecting device.

FIG. 1 is a configuration diagram showing a configuration of a sample substrate according to a first embodiment of the present invention. FIG. 2 is a top view showing the upper surface of the sample substrate according to the first embodiment. FIG. 2 is a cross-sectional view illustrating a cross section of the sample substrate according to the first embodiment, which is cut perpendicular to a longitudinal direction. FIG. 1 is a configuration diagram illustrating a configuration of a printer according to a first embodiment. FIG. 6 is a configuration diagram showing a configuration of a sample substrate according to a second embodiment of the present invention. FIG. 2 is a configuration diagram illustrating a configuration of a printer according to a second embodiment. FIG. 9 is a configuration diagram illustrating a configuration of a sample substrate according to a third embodiment of the present invention.

(First embodiment)
FIG. 1 is a configuration diagram showing a configuration of the sample substrate 10 according to the first embodiment of the present invention. FIG. 2 is a top view illustrating the upper surface of the sample substrate 10 according to the present embodiment. FIG. 3 is a cross-sectional view showing a cross section of the sample substrate 10 according to the present embodiment cut perpendicularly to the longitudinal direction. The same parts in the drawings are denoted by the same reference numerals, and different parts will be mainly described.

  The sample substrate 10 is used for a potential difference detecting device (oxidation-reduction potential detecting device) and is a substrate for generating a potential difference for measuring an oxidation-reduction potential (ORP) from a liquid sample to be tested. For example, the specimen is blood, but may be any liquid as long as it is liquid. The sample substrate 10 has a configuration in which a detection plate 1, a solution absorber 2, a sample absorber 3, and an electrode cover 4 are sequentially stacked from below. The sample substrate 10 is configured as a USB (universal serial bus) type. Here, the sample substrate 10 is described as a USB type configuration, but may be configured in any shape.

The oxidation-reduction potential is represented by the following equation.
Eh = Eo + (2.303RT / nF) · log ([Ox] / [Red]) ... Equation (1)
Here, Eh represents an oxidation-reduction potential, Eo represents a standard oxidation-reduction potential, R represents a gas constant, T represents an absolute temperature, F represents a Faraday constant, Ox represents an oxidized activity, and Red represents a reduced activity.

  The solution absorber 2 is made of a material that absorbs a solution in a spherical shape. The solution absorber 2 absorbs a solution for stabilizing the potential of the reference electrode 12. The solution absorber 2 is housed so as to fit into a depression provided on the surface (upper surface) of the detection plate 1. The height of the solution absorber 2 may be slightly higher than the depression of the detection plate 1. The depression may be a hole penetrating the detection plate 1. Since the solution absorber 2 has elasticity, the solution absorber 2 is accommodated in the depression by being pressed from above. For example, the solution is potassium chloride (KCl). Here, the spherical shape of the solution absorber 2 is not limited to a shape in which any arbitrary cross section is a perfect circle, and may be an ellipse such as an oval or a polyhedron. The solution absorber 2 is not limited to a spherical shape, and may have any shape such as a donut shape. Further, the material of the solution absorber 2 may be any material as long as it absorbs liquid. For example, the material of the solution absorber 2 is Japanese paper, cellulose, cloth, resin, cotton, or the like.

  The sample absorber 3 is provided so that the working electrode 11 and the reference electrode 12 on the surface of the detection plate 1 are entirely covered. The sample absorber 3 is a circular Japanese paper having a diameter of about 5 to 7 mm. The sample absorber 3 penetrates the sample dropped on the surface, and brings the sample into contact with the working electrode 11 and the reference electrode 12. The sample absorber 3 may be of any material and shape as long as it absorbs the sample and brings the sample into contact with the working electrode 11 and the reference electrode 12.

  The electrode cover 4 is attached so as to cover a portion other than the output terminal 16 of the sample substrate 10. An injection hole 41 for injecting a sample is provided on the surface of the electrode cover 4. The injection hole 41 is provided so that the reference electrode 12 is located immediately below. That is, if the injection hole 41 is located directly above the reference electrode 12 and the sample absorber 3 is not provided, when a sample is injected from the injection hole 41, the injection hole 41 is located at a position where the sample directly hangs on the reference electrode 12. For example, the injection hole 41 has a diameter of about 1 to 5 mm. Here, that the injection hole 41 is located directly above the reference electrode 12 does not necessarily mean that the center of the injection hole 41 and the center of the reference electrode 12 are coincident with each other. It is sufficient that the reference electrode 12 and the injection hole 41 partially overlap.

The detection plate 1 is a substrate for generating an oxidation-reduction potential from a specimen. The detection plate 1 includes a working electrode 11, a reference electrode 12, two wires 13 and 14, a reference electrode terminal 15, and a plurality of output terminals 16.
The working electrode 11 and the reference electrode 12 are electrodes that detect a potential difference for measuring an oxidation-reduction potential. The working electrode 11 generates a potential according to the components of the sample. The reference electrode 12 generates a reference potential for measuring the potential generated at the working electrode 11. The test of the sample is performed by measuring the oxidation-reduction potential based on the potential difference (potential difference) generated between the working electrode 11 and the reference electrode 12.

  The working electrode 11 is formed in a ring shape on the surface of the detection plate 1 so as to surround the reference electrode 12. The working electrode 11 is formed in a ring shape so as to surround the entire periphery of the reference electrode 12, so that the sample reaches the working electrode 11 even if there is a bias in the sample penetration direction in the sample absorber 3. The working electrode 11 does not have to be configured so as to surround the entire circumference of the reference electrode 12 (360 degrees) like a ring, but is configured to surround the circumference of the reference electrode 12 by 180 degrees or more. desirable. The reference electrode 12 preferably has a circular or arc shape, but may have a polygonal shape, an elliptical shape, or a partial shape thereof. The working electrode 11 is a metal electrode using gold. Gold is preferably pure gold. The working electrode 11 may be a metal electrode using platinum or antimony, or may be an electrode of another type such as a glass electrode.

  Reference electrode 12 is a silver / silver chloride electrode. The reference electrode 12 is one in which paste-like silver chloride is embedded in the bottom surface of the depression provided in the detection plate 1. The depth of this depression is, for example, about 2 mm. The solution absorber 2 is arranged so as to fit on the paste-like silver chloride in the depression. When the solution absorber 2 is pressed from above via the sample absorber 3, the solution absorber 2 comes into close contact with the silver oxide of the reference electrode 12. The reference electrode 12 may be any electrode as long as it is configured to generate a reference potential for measuring the oxidation-reduction potential.

The reference electrode terminal 15 is provided on the back side (lower side in FIG. 3) of the detection plate 1 so as to be always in contact with the paste-like silver chloride of the reference electrode 12 while being electrically connected thereto. The material of the reference electrode terminal 15 is, for example, gold.
The first wiring 13 electrically connects the working electrode 11 to one of the plurality of output terminals 16. For example, the first wiring 13 is provided as a pattern on the surface of the detection plate 1. The surface of the first wiring 13 is coated with an insulating paint so as to be electrically insulated from the outside.

  The second wiring 14 is electrically connected to the reference electrode terminal 15, and electrically connects the reference electrode 12 to one of the plurality of output terminals 16. For example, the second wiring 14 is provided as a pattern on the front surface and the back surface of the detection plate 1. Similarly to the first wiring 13, the surface of the second wiring 14 is coated with an insulating paint.

FIG. 4 is a configuration diagram illustrating a configuration of the printer 5 according to the present embodiment.
The printer 5 is a device that measures an oxidation-reduction potential from a sample substrate 10 and prints a test result of the sample based on the measured oxidation-reduction potential. The printer 5 is provided with a terminal (for example, a USB terminal) for connecting the sample substrate 10.

The printer 5 includes an arithmetic processing unit 51, an ORP amplifier 52, an operation unit 53, and an output unit 54.
The arithmetic processing unit 51 is a part that performs overall arithmetic processing in the printer 5. The arithmetic processing unit 51 performs measurement of the oxidation-reduction potential, arithmetic processing for inspecting a specimen based on the measurement result, control of devices or components constituting the printer 5, and the like. For example, the arithmetic processing unit 51 is a computer using a CPU (central processing unit) or the like.

  The ORP amplifier 52 is electrically connected to the working electrode 11 and the reference electrode 12 of the sample substrate 10 via the output terminal 16. The ORP amplifier 52 amplifies the potential difference generated between the working electrode 11 and the reference electrode 12, converts the amplified potential into a digital signal, and outputs the digital signal to the arithmetic processing unit 51.

  The operation unit 53 is a part for the operator who operates the printer 5 to make various inputs. For example, various inputs are input of information such as the start of inspection, the type of inspection, inspection conditions, or printer settings. The operation unit 53 may have a display function (display unit or the like) for displaying various information, such as a liquid crystal panel.

The output unit 54 performs printing in response to a command from the arithmetic processing unit 51. For example, the output unit 54 prints the inspection result calculated by the calculation processing unit 51 on a sheet. Note that the output unit 54 may display the inspection result on a display unit or the like without printing.
Next, an operation in which a specimen is tested by the potential difference detecting device according to the present embodiment will be described.

  When a sample (for example, blood) is injected into the injection hole 41 of the sample substrate 10, the sample penetrates the sample absorber 3 and spreads as a whole. As a result, the sample comes into contact with the working electrode 11 and the reference electrode 12, thereby generating an oxidation-reduction potential on the sample. The sample substrate 10 is connected to the printer 5 while the oxidation-reduction potential is generated. For example, the sample substrate 10 is connected to the printer 5 within about 30 seconds to 1 minute after the sample is injected into the sample substrate 10, and the oxidation-reduction potential is measured. Alternatively, the sample is injected into the sample substrate 10 after the sample substrate 10 is connected to the printer 5.

  The printer 5 measures a potential difference between two output terminals 16 electrically connected to the working electrode 11 and the reference electrode 12 of the sample substrate 10, respectively. Specifically, the potential difference between the two output terminals 16 is amplified by the ORP amplifier 52, converted into a digital signal, and input to the arithmetic processing unit 51. The arithmetic processing unit 51 measures a potential difference based on the input digital signal.

  The arithmetic processing unit 51 obtains an inspection result for the measured potential difference based on the determination procedure stored in the arithmetic processing unit 51 in advance. The arithmetic processing unit 51 outputs information on the obtained inspection result to the output unit 54. The output unit 54 prints the inspection result on a sheet based on the information received from the arithmetic processing unit 51.

According to the present embodiment, the configuration of the sample substrate 10 used for the potential difference detection device can be simplified.
In addition, by disposing the reference electrode 12 directly below the injection hole 41 on the sample substrate 10, the sample can be reliably brought into contact with the reference electrode 12.
Further, by disposing the working electrode 11 so as to surround the reference electrode 12, even if there is a bias in the direction in which the sample penetrates into the sample absorber 3 due to individual differences, the sample can be easily brought into contact with the working electrode 11. can do. Therefore, it is desirable that the working electrode 11 be provided so as to surround the reference electrode 12 by 180 degrees or more.

  In addition, contrary to the present embodiment, even if the working electrode 11 is arranged at the center and the reference electrode 12 is arranged so as to surround the working electrode 11, the same operation and effect as in the present embodiment can be obtained. .

(Second embodiment)
FIG. 5 is a configuration diagram showing a configuration of the sample substrate 10A according to the second embodiment of the present invention.
The sample substrate 10A is obtained by adding the pH working electrode 17 and the third wiring 18 to the sample substrate 10 according to the first embodiment shown in FIG. 1 and replacing the detection plate 1 with the detection plate 1A. The detection plate 1A is obtained by replacing the working electrode 11 in the detection plate 1 with a working electrode 11A. Other points are the same as those of the sample substrate 10 according to the first embodiment.

  When the detection electrode 1A is viewed from above, the working electrode 11A has an arc shape or a horseshoe shape in which a ring-shaped portion is missing. The working electrode 11A is provided for an oxidation-reduction potential. Other points are the same as the working electrode 11 according to the first embodiment. Therefore, as in the first embodiment, the shape of the working electrode 11A is not limited to the above-described shape, but the following description will be made assuming that the ring-shaped part is partially missing.

  When the pH working electrode 17 is connected to the reference electrode 12 by a straight line, the pH working electrode 17 is disposed at a position where the connected straight line passes through a part of the working electrode 11A where a ring-shaped part is missing. That is, the straight line connecting the pH working electrode 17 and the reference electrode 12 does not overlap with the working electrode 11A. The working electrode 17 for pH is disposed at a position where the sample absorber 3 covers when the center of the sample absorber 3 is the reference electrode 12.

  The working electrode 17 for pH is an electrode for detecting a potential difference for measuring pH together with the reference electrode 12. That is, the reference electrode 12 is used as a reference potential for measuring the oxidation-reduction potential and the pH, respectively. The pH working electrode 17 generates an electric potential according to the components of the sample. The pH of the sample is measured based on the difference in potential (potential difference) generated between the working electrode 17 for pH and the reference electrode 12. The working electrode 17 for pH is a metal electrode using antimony. The pH working electrode 17 may be a metal electrode using gold or platinum, or may be another type of electrode such as a glass electrode.

  The third wiring 18 electrically connects the pH working electrode 17 to one of the plurality of output terminals 16. For example, the third wiring 18 is provided as a pattern on the surface of the detection plate 1. Similarly to the first wiring 13 according to the first embodiment, the surface of the third wiring 18 is coated with an insulating paint. The second wiring 14 is the same as that of the first embodiment, but may be provided so as to connect the reference electrode 12 to the two output terminals 16 and may be selectively used for measuring the oxidation-reduction potential and measuring the pH.

FIG. 6 is a configuration diagram illustrating a configuration of the printer 5A according to the present embodiment.
The printer 5A has a configuration in which a pH amplifier 55 is added to the printer 5 according to the first embodiment shown in FIG. 4 and the arithmetic processing unit 51 is replaced with an arithmetic processing unit 51A. Other points are the same as those of the printer 5 according to the first embodiment.

  The pH amplifier 55 is electrically connected to the pH working electrode 17 and the reference electrode 12 of the sample substrate 10 via the output terminal 16. The pH amplifier 55 amplifies the potential difference between the working electrode 17 for pH and the reference electrode 12 to measure the potential difference, converts the amplified potential into a digital signal, and outputs the digital signal to the arithmetic processing unit 51A. The pH amplifier 55 and the ORP amplifier 52 may be configured to select either one by a switch.

  The arithmetic processing unit 51A performs arithmetic processing for measuring a pH or for testing a specimen based on the measurement result. The arithmetic processing unit 51A outputs information on the pH measurement result or the obtained test result to the output unit 54. Other points are the same as those of the arithmetic processing unit 51 according to the first embodiment.

According to the present embodiment, in addition to the function and effect according to the first embodiment, the pH can be measured.
The working electrode 11, the reference electrode 12, and the working electrode 17 for pH may be arbitrarily interchanged in position or shape, and the material may be arbitrarily selected.

(Third embodiment)
FIG. 7 is a configuration diagram showing a configuration of the sample substrate 10B according to the third embodiment of the present invention.
The sample substrate 10B is obtained by replacing the detection plate 1 with the detection plate 1B in the sample substrate 10 according to the first embodiment shown in FIG. The detection plate 1B is obtained by replacing the working electrode 11 in the detection plate 1 with a working electrode 11B. Other points are the same as those of the sample substrate 10 according to the first embodiment.

The working electrode 11B is the same as the working electrode 11 according to the first embodiment except that the working electrode 11B has a different shape and arrangement from the working electrode 11 according to the first embodiment.
The working electrode 11 </ b> B is provided near the reference electrode 12 on the surface of the detection plate 1. For example, the working electrode 11B extends in the longitudinal direction of the detection plate 1 and is provided such that a straight line passing through the center of the reference electrode 12 or a straight line passing through the center of the detection plate 1 passes through the center of the working electrode 11B. The working electrode 11B may be provided at any position as long as it is within a range covered by the sample absorber 3. The shape of the working electrode 11B is, for example, a circle or an ellipse, but may be any shape.

According to the present embodiment, by providing the working electrode 11B near the reference electrode 12, even if there is a slight bias in the direction in which the sample penetrates into the sample absorber 3, the working electrode 11B is almost equivalent to the first embodiment. The sample can be brought into contact with the working electrode 11B.
For example, as shown in FIG. 7, the sample absorber 3 is formed in a circular shape such that the injection hole 41 is formed immediately above the center, and the working electrode 11 </ b> B is provided within a range covered by the sample absorber 3. By penetrating the absorber 3 so as to spread concentrically, the specimen can almost certainly come into contact with the working electrode 11B.

With such a configuration, the working electrode 11B does not need to have a shape surrounding the reference electrode 12, and can be formed into an arbitrary shape. Therefore, the working electrode 11B can be manufactured more easily than the first embodiment.
Note that the present invention is not limited to the above-described embodiment, and components may be deleted, added, or changed. Further, a new embodiment may be achieved by combining or exchanging constituent elements of a plurality of embodiments. Even if such an embodiment is directly different from the above-described embodiment, those having the same purpose as the present invention are described as the embodiment of the present invention, and the description thereof is omitted.

  DESCRIPTION OF SYMBOLS 1 ... Detection plate, 2 ... Solution absorber, 3 ... Sample absorber, 4 ... Electrode cover, 10 ... Sample substrate, 11 ... Working electrode, 12 ... Reference electrode, 13, 14 ... Wiring, 16 ... Output terminal.

Claims (8)

A sample substrate used for a potential difference detection device,
A first electrode for measuring a first potential difference for the analyte;
A second electrode for measuring the first potential difference;
A cover in which the sample injection hole is located directly above the first electrode;
A sample absorbing means for absorbing the sample when the sample is injected from the injection hole and bringing the sample into contact with the first electrode and the second electrode; substrate. The specimen substrate according to claim 1, wherein the second electrode is arranged so as to surround the first electrode by 180 degrees or more. The specimen substrate according to claim 1, wherein the first electrode is an electrode for measuring a reference potential of the first potential difference. The specimen substrate according to claim 1, wherein the first potential difference is a potential difference for measuring an oxidation-reduction potential. 2. The sample substrate according to claim 1, further comprising a solution absorber provided to be in contact with the first electrode and absorbing a solution for stabilizing a potential of the first electrode. 3. The specimen substrate according to claim 1, further comprising a third electrode for measuring a second potential difference of the specimen. The specimen substrate according to claim 6, wherein the second potential difference is a potential difference for measuring pH. A sample substrate for injecting the sample,
Test result output means for outputting a test result of the sample,
The specimen substrate,
A first electrode for measuring a first potential difference for the sample,
A second electrode for measuring the first potential difference;
A cover in which the sample injection hole is located directly above the first electrode;
When the sample is injected from the injection hole, the sample absorbs the sample and includes a sample absorber for bringing the sample into contact with the first electrode and the second electrode. Detection device.

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