JP2018095609A - Composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a composition that can improve nutritional state and has excellent anti-obesity effects.SOLUTION: The composition of the present invention is an oral composition containing a green leaf processed product and a high molecular weight polysaccharide. The value of Line Spread Test when adjusted to a solution in which the composition is dispersed or dissolved in water so that the solid content is 10% by mass or more and 15% by mass or less is 25 mm or more and 60 mm or less. It is preferable that the high molecular weight polysaccharide is at least one selected from xanthan gum and derivatives thereof, starch and derivatives thereof, and salts thereof.SELECTED DRAWING: None

Description

  The present invention relates to a composition comprising a processed green leaf product and a high molecular polysaccharide.

  Conventionally, compositions containing processed green leaves are known as health foods such as green juice. The importance of ingesting vegetables has attracted attention due to recent health consciousness and disturbances in eating habits, and compositions containing processed green leaves are known as one of the means for easily ingesting a certain amount of vegetables. (For example, patent document 1).

JP 2014-155437 A

On the other hand, the needs of consumers for health foods including compositions containing processed green leaf products have been diversified in recent years, and there is a demand for further improvement of the effect, particularly a request for further improvement of the health maintenance viewpoint. Is getting stronger and stronger.
However, the composition containing the conventional green leaf processed product does not sufficiently meet this requirement.

  Then, this inventor earnestly examined about the structure in which the further effect | action reinforcement | strengthening is obtained regarding health maintenance about the composition containing a green leaf processed material. As a result, surprisingly, a composition containing a high molecular polysaccharide in addition to a processed green leaf product and having a specific range of fluidity when used as an aqueous dispersion with a specific concentration is excellent in improving nutritional status or anti-obesity. As a result, the present invention has been completed.

  The present invention is based on the above findings, and is an oral composition containing a processed green leaf product and a high molecular polysaccharide, and is adjusted to an aqueous dispersion or solution having a concentration of 10% by mass to 15% by mass. A composition having a line spread test (hereinafter also referred to as “LST”) value of 25 mm or more and 60 mm or less is provided.

  ADVANTAGE OF THE INVENTION According to this invention, the composition which not only is excellent in a nutritional condition improvement or an anti-obesity effect but can satisfy both simultaneously can be provided.

Hereinafter, the composition of this invention is demonstrated based on the preferable embodiment. The composition of this embodiment is an oral composition.
The composition of the present embodiment includes a processed green leaf product. The green leaf in the green leaf processed product is not particularly limited as long as it is a leaf of a green plant suitable for food and drink applications. Gramineae plants such as sorghum and sugar cane; Asteraceae plants such as mugwort; Aceraceae plants such as Ashitaba, parsley, celery, button boowe; Mulaceae plants such as mulberry; Examples include Labiatae plants; cruciferous plants such as komatsuna, kale, cabbage, broccoli; liliaceae plants such as asparagus; lindenaceae plants such as Morohea; convolvulaceae plants such as sweet potato Can be used as food ingredients such as green juice, barley such as Nijo barley, Rokujo barley, bare barley, mugwort, ashitaba, button bow fu, mulberry, Lumpur, is a sweet potato. As green leaves, one or a combination of two or more of these can be used. The green leaf may include not only the leaf portion of the plant body but also the stem and other portions together with the leaf.

  In the case of barley, the green leaves are preferably harvested before the maturity period, that is, from the beginning of the splitting to the early stage of heading.

  The green leaves are preferably processed immediately after harvesting. When time is required for processing, it is stored by storage means commonly used by those skilled in the art, such as cold storage, in order to prevent deterioration of green leaves.

  In the composition of the present embodiment, various processed products obtained from the green leaves can be used as the green leaves. Examples of such processed products include dry powders of green leaves, fragmented green leaves and dry powders thereof, squeezed green leaves and dry powders thereof, green leaf extracts and dry powders thereof, and the like.

  For example, conventionally known methods can be used to dry green leaves. As such a method, a method combining drying treatment and pulverization treatment with respect to green leaves can be used. Either the drying process or the pulverization process may be performed first, but the drying process is preferably performed first. Dry powdering may be combined with one or more treatments selected from treatments such as blanching treatment and sterilization treatment, if necessary. In addition, the number of times of pulverization may be one time or a combination of two or more processes, but it is preferable to combine a fine pulverization process that finely pulverizes after the coarse pulverization process.

  The blanching process is a process for keeping the green color of the green leaves vivid, and examples of the blanching process include a hot water process and a steaming process. In the blanching treatment, the green leaves are preferably treated in hot water or steam at 80 to 100 ° C., preferably 90 to 100 ° C. for 60 to 180 seconds, preferably 90 to 120 seconds. In addition, when performing hydrothermal treatment as a blanching treatment, the green color of green leaves can be made more vivid by dissolving carbonates such as magnesium carbonate and hydrogen carbonates such as sodium bicarbonate in hot water. This is preferable because it is possible. Moreover, as a steaming process, the intermittent steaming process which repeats the process which steams a green leaf with water vapor | steam, and the process which cools under normal pressure or pressurization is preferable. In the intermittent steaming process, the steaming process is preferably performed for 20 to 40 seconds, more preferably 30 seconds. The cooling treatment after the steaming treatment is preferably performed immediately, and the method is not particularly limited, but the method is not particularly limited, soaking in cold water, refrigeration, cooling with cold air, evaporative cooling with hot air, evaporative cooling combining hot air and cold air Etc. are used. Among these, evaporative cooling combining hot air and cold air is preferable. Such cooling treatment is performed so that the temperature of the green leaves is preferably 60 ° C. or lower, more preferably 50 ° C. or lower, and most preferably 40 ° C. or lower. Moreover, in order to produce a green leaf powder rich in nutritional components such as vitamins, minerals, and chlorophyll, it is preferable to repeat the intermittent steaming treatment 2 to 5 times.

  The sterilization treatment is usually a treatment for physically and chemically killing microbial cells using temperature, pressure, electromagnetic waves, chemicals and the like. When the branching process is performed in addition to the drying process and the pulverization process, the branching process is preferably performed before the drying process. Moreover, when performing a sterilization treatment in addition to the drying treatment and the pulverization treatment, the sterilization treatment is preferably performed after the drying treatment or before or after the pulverization treatment.

  The drying treatment is preferably a treatment for drying so that the water content of the green leaves is 10% by mass or less, particularly 5% by mass or less. This drying treatment can be performed by any method known to those skilled in the art, such as hot air drying, high pressure steam drying, electromagnetic wave drying, freeze drying, and the like. Drying by heating can be performed at a temperature and a time at which green leaves are not discolored by heating, preferably at 40 ° C to 140 ° C, more preferably 80 to 130 ° C.

  Examples of the pulverization treatment include a pulverization treatment by an arbitrary method commonly used by those skilled in the art using a crusher, a mill, a blender, a stone mortar or the like. The pulverized green leaves are sieved as necessary.

  As a specific dry powdering method, for example, after cutting barley green leaves, a blanching treatment is performed, followed by drying so that the water content is 10% by mass or less, preferably 5% by mass or less. The method of grind | pulverizing is mentioned (refer Unexamined-Japanese-Patent No. 2004-000210). Further, for example, a method of cutting green leaves, performing branching treatment, then twisting, and then drying and pulverizing (see Japanese Patent Application Laid-Open No. 2002-0665204 and Japanese Patent No. 3428956) can also be mentioned. Further, for example, a method of drying green leaves, coarsely pulverizing them, heating them at 110 ° C. or higher, and further finely pulverizing them (see Japanese Patent Application Laid-Open No. 2003-033151 and Japanese Patent No. 3277181) can be mentioned.

  As a method for fragmenting the green leaves, methods commonly used by those skilled in the art to fragment a plant body, such as slicing, crushing, and chopping, can be used. As an example of fragmentation, a slurry may be used. Slurry is performed by putting barley green leaves through a mixer, a juicer, a blender, a mascoloider, etc., and making the barley green leaves into a cocoon (liquid and solid suspension). By slurrying in this way, the green leaves are preferably 80% by mass or more of the strips, preferably having an average diameter of 1 mm or less, more preferably 0.5 mm or less, even more preferably 0.1 mm or less, and most preferably 0.05 mm. It is made into pieces and has fluidity.

  Examples of the method of squeezing green leaves include a method of squeezing green leaves or a fragmented product thereof, or a method of centrifuging or filtering a fragmented product of barley green leaves. A typical example is a method of squeezing juice by a mechanical crushing means such as a mixer or a juicer, and obtaining a squeezed liquid by removing coarse solids by means such as sieving or filtration, if necessary. . Specific examples include the methods described in JP-A-08-245408, JP-A-09-047252, JP-A-5-7471, JP-A-4-341153, and the like. Can be appropriately selected and implemented by those skilled in the art.

  As a method for obtaining a green leaf extract, an extraction solvent usually used by those skilled in the art, such as ethanol, water, hydrous ethanol, or the like, is added to green leaf or a fragmented product thereof, and extraction is performed by stirring or heating as necessary. be able to. The extract may be concentrated as necessary.

  Among the processed green leaves, it is particularly preferable to use a dry powder of green leaves (also referred to as pulverized powder) from the viewpoint of more easily obtaining nutritional effects and anti-obesity effects.

  In particular, the green leaf processed product is preferably a powder that passes through any sieve having a particle size of 30 to 250 mesh because uniform mixing of the green leaf powder and the polymer polysaccharide is easy. From the same viewpoint, it is more preferable that 90% by mass or more of the green leaf powder passes through 200 mesh.

  From the viewpoint of further enhancing the effects of the above-described nutritional improvement and anti-obesity, the content of the processed green leaf in the solid content of the composition is preferably 0.1% by mass or more, and 0.5% by mass or more in terms of dry mass. More preferably, it is more preferably 1% by mass or more, particularly preferably 3% by mass or more, particularly preferably 5% by mass or more, and most preferably 10% by mass or more. From the same viewpoint, the upper limit is preferably 99.9% by mass or less, more preferably 99% by mass or less, still more preferably 97% by mass or less, particularly preferably 90% by mass or less, and particularly preferably 75% by mass or less. Preferably, 50 mass% or less is the most preferable.

  The composition contains a polymeric polysaccharide. As a high molecular polysaccharide, it can be used as an edible material, and starch, other natural polysaccharides or derivatives thereof which are usually used as a thickener can be used. Specific high molecular polysaccharides include starch, dextrin, xanthan gum, guar gum, succinoglycan, carrageenan, locust bean gum, galactan, gum arabic, tragacanth gum, tamarind gum, psyllium seed gum, agar, agarose, mannan, curdlan , Alginic acid or salts thereof, gum arabic, pectin, quinseed, algae colloid, alkathylan, chitosan and derivatives thereof, hyaluronic acid or salts thereof, mucopolysaccharides such as chondroitin sulfate, and the like. A high molecular polysaccharide can also be used 1 type or in combination of 2 or more types among these. Among them, xanthan gum and derivatives thereof, starch and derivatives thereof, guar gum and derivatives thereof, tamarind gum and derivatives thereof, pectin and derivatives thereof, psyllium seed gum and derivatives thereof, and salts thereof are used later. It is preferable because the value of LST can be easily adjusted to a specific range described later. Examples of the xanthan gum derivative include cationized xanthan gum. Examples of the starch include starch derived from various plants such as corn, waxy corn, potato, sweet potato, tapioca, sago, wheat, rice, and glutinous rice. As starch derivatives, starches such as pregelatinized starch, solubilized starch, etherified starch, esterified starch, cross-linked starch, and grafted starch, which have been subjected to chemical and physical treatment, can be used. Examples of pregelatinized starch include starch that is gelatinized by heating with water and dried in that state. Examples of the guar gum derivative include hydroxypropyl guar gum, carboxymethylhydroxypropyl guar gum, and cationized guar gum. Examples of the salt include alkali metal salts such as sodium salt and potassium salt.

The composition of this embodiment contains a green leaf processed product and a high molecular polysaccharide, and has a specific fluidity when it is an aqueous dispersion or aqueous solution having a solid content of 10 to 15% by mass. It is a feature. As a result of earnestly examining the relationship between the composition of the composition containing the green leaf processed product and its functionality, the present inventors have found that the fluidity when containing the high molecular weight polysaccharide is in a specific range, It has been found that not only the nutrition improvement effect in the case of and the anti-obesity effect in a high nutrition state are excellent, but both are satisfied simultaneously.
Specifically, in the composition of the present embodiment, the LST value of the aqueous dispersion is from 25 mm to 60 mm, more preferably from 26 mm to 55 mm, and particularly preferably from 27 mm to 50 mm. preferable.

In the LST, a sample is provided at the center of a measurement plate in which the distance from the center is displayed in a concentric manner, the distance at which the sample spreads within a predetermined time is measured, and the value is used as a thickening index. LST includes a method using a ring (cutting die) and a method using a syringe. In this embodiment, a method using a ring is adopted. Specifically, it is performed by the methods (1) to (3). The following (1) to (3) are performed at room temperature of 1 ° C. or higher and 30 ° C. or lower, preferably 20 ° C. or higher and 30 ° C. or lower.
(1) Place the measurement plate on a horizontal place, and use the measurement ring (Saraya Inc .: Simple Thickness Measurement Version (Line Spread Test) Set, Inner Diameter 3 cm, Capacity 20 mL, the position of the central axis is the center of the measurement plate Place on the measuring plate to match.
(2) Pour 20 mL of sample into the ring using a 100 mL beaker.
(3) After the flowed specimen spreads evenly in the ring, the ring is lifted vertically. The distance that the specimen spreads 1 minute after lifting (the distance between the position farthest from the center of the measurement plate and the center of the measurement plate among the portions where the specimen is present) is measured, and this is taken as the LST value. The LST value is the average of the values measured 10 times by the above method.

  Preparation of specimens for measurement is basically performed without heating. Specifically, for example, when the composition to be subjected to LST measurement is in a solid state, a dispersion liquid in which the solid content is dispersed or dissolved so as to have a concentration of 10 to 15% by mass is used as a specimen. In this case, for example, when the composition is in the form of powder or granules passing through 30 mesh, water is used as a means of dispersion or dissolution, and decomposition or dissolution treatment is performed at room temperature (1 to 30 ° C.). The LST test is performed within 60 minutes after the dispersion or dissolution treatment.

  On the other hand, the composition is in a solid state that does not pass 30 mesh, such as a tablet shape, a rod shape, a plate shape, a block shape, a solid shape, a round shape, a bowl shape, a tablet shape, a gummy shape, a wafer shape, a biscuit shape, a cookie shape, In the case of cake, chewable, syrup, stick, etc., this is pulverized and passed through 30 mesh, preferably into powder that does not pass 300 mesh, and then subjected to the same dispersion or dissolution treatment as above. And subject to the LST test within the aforementioned time from the treatment. Examples of the pulverization treatment in this case include dry pulverization. As a specific dry pulverization method, pulverization using a hammer mill, roll mill, ball mill, jet mill, or the like is preferably performed.

  On the other hand, when the composition is in a shape other than solid, for example, syrup, liquid, gel, jelly, paste, cream, it is dried to form a solid, and if necessary, the above After the same dry pulverization as above and passing through 30 mesh, preferably into a powder that does not pass 300 mesh, this is subjected to the same dispersion or dissolution treatment as described above. Take the test. Examples of the drying process in this case include a process of drying to a moisture content of 15% by mass or less using spray drying or freeze drying.

  When the composition is in the form of capsules or caplets such as hard capsules or soft capsules, the contents taken out from the capsules are dried or pulverized as necessary, or dispersed or dissolved without being processed. The sample is subjected to LST measurement.

  A composition having an LST in the above range can be obtained by adjusting the type and amount of the polymeric polysaccharide. The kind of high molecular polysaccharide is as above-mentioned. Further, the amount of the high molecular polysaccharide in the composition is preferably 0.1% by mass or more and 99.9% by mass or less as a dry mass in the solid content of the composition, and preferably 1% by mass or more and 99% by mass or less. More preferably, it is more preferably 2% by mass or more and 98% by mass or less, further preferably 2.5% by mass or more and 90% by mass or less, and more preferably 5% by mass or more and 80% by mass or less. It is particularly preferable that the content be 10% by mass or more and 70% by mass or less, and it is most preferable that the content be 20% by mass or more and 60% by mass or less.

In the present embodiment, LST may be measured with a solution or dispersion having a solid content concentration of 10% by mass or more and 15% by mass or less, and LST may be measured at any concentration within the range. Although it may be in the above range, it is preferably measured for a solution or dispersion having a solid content in the range of 11% by mass to 14% by mass, more preferably 11.5% by mass to 13% by mass. Measure for a range of solution or dispersion.
In addition, solid content here is the mass after drying for 24 hours at 30 degreeC or more and 50 degrees C or less in the state of humidity (RH) 40-60%, when disperse | distributing or melt | dissolving a solid substance in water. To do.

  The composition of the present embodiment may contain other components in addition to the green leaves and the polymer polysaccharide. Examples of the other components include vitamins, proteins, oligosaccharides, minerals, dairy products, processed plant products, microorganisms such as lactic acid bacteria, sugars, sweeteners, citric acid, acidulants, colorants, and brighteners. In addition, production agents such as talc, silicon dioxide, cellulose, and calcium stearate can be blended. Examples of other components include various excipients, binders, lubricants, stabilizers, diluents, extenders, emulsifiers, coloring agents, fragrances, food additives, seasonings and the like. Can do. The content of other components can be appropriately selected according to the form of the composition. In this embodiment, it is preferable that components other than the green leaf processed material and polymer polysaccharide contained in a composition are 99 mass% or less in solid content, and it is especially preferable that it is 97 mass% or less.

  The composition of this embodiment can be in the various forms described above. Moreover, as a composition of this embodiment, it is preferable to use as food-drinking compositions, such as a green juice and a smoothie, and it is especially preferable to use as a food-drinking composition for green juices. The composition for eating and drinking for green juice is a beverage containing green leaves as various processed products, and the composition for eating and drinking for green juice is a solid that is dispersed or dissolved in a liquid to obtain this beverage and this beverage. Is mentioned. In particular, when the composition is in the form of powder or granules and is ingested orally with a mixture mixed with water, it is suitable for long-term storage while preventing spoilage, and when the composition for eating and drinking is mixed with water. Is preferable because it is vivid. Further, when the composition is in a solid form, as described above, it is made into a liquid mixed with water, and can be taken orally, such as drinking the liquid. Accordingly, it may be taken orally as a solid. Further, not only water but also milk, soy milk, fruit juice drink, whey drink, soft drink, yogurt, hot cake mix and the like may be used. Moreover, it cannot be overemphasized that you may use as a supplement, health food, functional nutrition food, functional indication food, food for specified health, and a pharmaceutical.

  The composition of the present embodiment, as described in the examples described later, includes both a green leaf processed product and a high molecular weight polysaccharide and fulfills the LST value in the above-described range, thereby providing both a nutritional state improvement effect and an anti-obesity effect. Can do. Specifically, by ingesting the composition of this embodiment under low nutrient conditions such as low protein and low calcium, the protein mass and calcium content in the blood can be maintained or increased, and the low protein Undernutrition such as condition and low calcium condition is improved. Moreover, weight loss can be suppressed by orally ingesting the composition of this embodiment under low nutrient conditions such as low protein and low calcium. In addition, in a satiety state or an overnutrition state, by taking the composition of this embodiment orally, weight gain can be suppressed and obesity can be prevented or reduced. Therefore, the composition of this embodiment can suppress an increase or decrease in body weight due to malnutrition or overnutrition due to disturbance of eating habits by ingesting this, and is effective in physical condition management and health maintenance. Therefore, for example, the composition of the present embodiment can be taken orally to prevent and improve undernutrition such as aging, unbalanced diets, hypoproteinosis and hypocalcemia associated with extreme diets, You can easily limit your weight while maintaining your health. In particular, elderly people are prone to hypoproteinosis and hypocalcemia due to a decrease in appetite, a decrease in food intake, a biased diet, etc., and the composition of this embodiment is used to suppress or improve such symptoms. it can. Furthermore, conventionally, when dietary health is restricted by dieting and health food is ingested in a low nutritional state, the lack of nutrition has not been improved. However, according to the composition of the present embodiment, it is possible to achieve both nutritional status improvement and obesity prevention or improvement, making it easy to diet while maintaining health. In addition, since the composition of the present invention has both nutritional status improvement and obesity prevention or improvement action, it can be taken in a sufficient amount even during dieting, and the effect of improving vegetable shortage due to green leaves can be expected. The composition of this embodiment is used for one or more uses selected from anti-obesity, nutrition improvement, hypoproteinemia prevention or improvement, hypocalcemia prevention or improvement, and vegetable deficiency improvement. be able to.

  EXAMPLES Hereinafter, although an Example is shown and this invention is demonstrated further more concretely, the scope of the present invention is not limited to these Examples.

[Examples 1 to 4 and Comparative Examples 1 to 5]
As the green leaf processed product, the dry powder of barley green leaf produced in Production Example 1 below was used. As the polymer polysaccharide, pregelatinized starch in which a 5% by mass aqueous solution exhibits a viscosity of 100 to 600 mPa · s at 30 ° C. or xanthan gum in which a 1% by mass aqueous solution exhibits a viscosity of 500 to 2000 mPa · s at 25 ° C. was used. Only the dry powder of green leaves was used as the composition of Comparative Example 1.
Moreover, the composition of Comparative Examples 2 and 3 and Examples 1-3 was a mixture containing green leaf dry powder and starch, and the composition of Example 4 was a mixture containing green leaf dry powder and xanthan gum. The composition of Comparative Example 4 was starch only, and the composition of Comparative Example 5 was xanthan gum only. The compositions of Examples 1 to 4 and Comparative Examples 1 to 5 were all in powder form and passed through a 30-mesh sieve and did not pass through a 250-mesh sieve. In the blending of the green leaf dry powders of the compositions of Comparative Examples 2 and 3 and Examples 1 to 4 and starch or xanthan gum, the LST of the aqueous dispersion or solution having a solid content of 12% by mass is the value shown in Table 1 below. It was a ratio. LST uses a measuring plate and a measuring ring of a simple thickening measurement version (line spread test) set manufactured by Saraya Co., Ltd. at room temperature (20 ° C. or higher and 30 ° C. or lower) for an aqueous dispersion or solution having a solid content of 12% by mass for each sample. Measured. Dispersion or dissolution treatment in water for preparing specimens from the powder compositions of each Example and Comparative Example was performed by natural dissolution at room temperature for 0.5 hours.

(Production Example 1)
As a raw material, green leaves containing barley stems cut before heading were used. This was washed with water to remove adhering mud and the like, and a pretreatment was performed to cut it to a size of about 5 to 10 cm. The pretreated green leaves were blanched only once with hot water at 90 to 100 ° C. for 90 to 120 seconds, and then cooled with cold water. Subsequently, the obtained green leaves were dried with warm air at 80 ° C. to 130 ° C. for 20 minutes to 180 minutes in a dryer until the water content became 5% by mass or less. The dried green leaves were roughly crushed to a size of about 1 mm. The obtained green leaves of barley were finely pulverized so that 90% by mass or more passed through the 200 mesh section to obtain dry powder samples of green leaves. The green leaf powder sample that passed through 200 mesh was 90% by mass or more.

The powder compositions of Examples 1 to 4 and Comparative Examples 1 to 5 were subjected to the following animal test 1.
[Animal test 1: weight gain in a fed state]
Male KK-Ay mice (21-25 weeks old) were allowed to acclimatize for 2 days or more, and then body weight was measured on test day 0 and divided into 10 groups. Group 10 was an intake group for each of the control group 1 and the compositions of Examples 1 to 4 and Comparative Examples 1 to 5. In the acclimatization period, MF powder feed (produced by Oriental Yeast Co., Ltd.) was freely ingested. For 3 days from the 0th day of the test, the control group was fed with MF powder feed, and each of the other 9 groups was fed with a mixture of 3% by weight of the corresponding composition (test substance) and 97% by weight of MF powder feed. . The body weight was measured again in the afternoon on the third day of the test, and the weight fluctuation g1 = the weight on the third day of the test−the weight on the 0th day of the test was determined and shown in Table 1. Also, based on the weight fluctuation g1, those evaluated as having a high weight gain inhibitory effect are indicated as ◎, those evaluated as having a slightly high weight gain inhibitory effect are indicated as ◯, and those evaluated as having a low weight gain inhibitory effect are indicated as ×. It is shown in 1.

  As shown in Table 1, the composition of each Example containing a green leaf processed product and a polymer polysaccharide and having an LST of 25 mm or more and 60 mm or less does not contain any one of the green leaf processed product and the polymer polysaccharide. Even if both were contained, LST was superior to the compositions of Comparative Examples 1 to 5 outside the scope of the present invention, and was excellent in suppressing weight gain when ingested under satiety conditions without nutritional restrictions.

Furthermore, the powdery compositions of Examples 1 to 4 and Comparative Examples 1 to 5 were subjected to the following animal test 2.
[Animal test 2: weight gain / decrease, blood protein concentration, Ca concentration in undernutrition]
Male C57BL / 6J (DIO) mice (11-14 weeks old) were provided with a acclimation period of 2 days or more as described above, and then body weight was measured on test day 0 and divided into 10 groups. Group 10 was an intake group for each of the control group 1 and the compositions of Examples 1 to 4 and Comparative Examples 1 to 5. For 3 days from the 0th day of the test, the control group is fed the test feed (low protein diet) having the composition shown in Table 2 below, and the other 9 groups each have a corresponding composition (test substance) of 3.5% by mass. A 96.5% by weight mixture of the test feed was ingested. Body weight was measured in the afternoon of test day 3, and Δg2 = weight on test day 3−weight on test day 0 was determined. In addition, blood was collected from the tail vein and serum was collected, and the protein concentration in serum and the calcium concentration in serum were measured using a commercially available kit. Table 3 shows body weight fluctuation g2, protein concentration in serum, and calcium concentration in serum. In Table 3, the structural components and LST of the powdery compositions of Examples 1 to 4 and Comparative Examples 1 to 5 shown in Table 1 are shown again.
In Table 3, based on the weight fluctuation g2, those evaluated as having a high weight loss inhibitory effect are indicated by ◎, those evaluated as slightly high by ◯, and those evaluated by low as x.
In addition, based on the serum protein concentration, ◎ indicates that the serum protein decrease inhibitory effect is high, ◯ indicates that it is evaluated as slightly high, and x indicates that it is evaluated as low.
In addition, based on the serum Ca concentration, those evaluated as having a high serum Ca decrease inhibitory effect are indicated by ◎, those evaluated as slightly high by ◯, and those evaluated by low, by ×.

  As shown in Table 3, the composition of each example is superior to the composition of each comparative example in the effect of suppressing weight loss when ingested under a low protein diet condition, and under the low protein diet condition. The serum protein mass and the serum calcium content in were higher than the compositions of the respective comparative examples.

From the above, it can be seen that the composition of the present invention is excellent in both nutritional status improvement and anti-obesity effects.

Claims (3)

  An oral composition containing a processed green leaf product and a high molecular weight polysaccharide, and the line spread test when adjusted to a liquid dispersed or dissolved in water so as to have a solid content of 10% by mass to 15% by mass Is a composition having a value of 25 mm or more and 60 mm or less.   The high molecular polysaccharide is at least one selected from xanthan gum and derivatives thereof, starch and derivatives thereof, guar gum and derivatives thereof, tamarind gum and derivatives thereof, pectin and derivatives thereof, psyllium seed gum and derivatives thereof, and salts thereof The composition of claim 1. The composition according to claim 1 or 2, which is used for anti-obesity, nutrition improvement, or vegetable deficiency improvement.