JP2017184737A - MFD inspection support system and inspection method using MFD - Google Patents

Abstract

PROBLEM TO BE SOLVED: To support the execution of an inspection using MFD at the site, and to appropriately notify and instruct the site of the judgment content for the inspection result and the response content based on the judgment content. SOLUTION: When the sample is unknown, a drug for sample identification is introduced into the MFD 40, and when the sample is known, a drug for sensitivity comparison is introduced, and the drug is pre-encapsulated. If so, the corresponding culture condition information is displayed, and the inspection device 30 is specified by the culture condition selected from a plurality of preset culture conditions or the culture condition information displayed on the MFD 40. Under the same culture conditions, the stored MFD 40 is cultured, the MFD 40 being cultured is photographed, image data, drug data, and culture condition data are transmitted to the determination server 10, and the determination server 10 is based on the image data. The sample is identified and / or the effect of the drug is determined, and the determination result is transmitted to the inspection device 30. [Selection diagram] Fig. 1

Description

  The present invention relates to an inspection technique using a microfluidic device (MFD), and in particular, an MFD inspection support system and an MFD that automatically make a determination based on data of an inspection performed on-site using an MFD. The present invention relates to a technique that is effective when applied to an inspection method that uses the above.

  As one of biochemical analysis substrates (biochips), micrometer (μm) level flow paths are used to perform drug sensitivity assessment, reagent analysis, reactivity assessment, etc. in a short time. Development of a microchannel device / microchannel chip (hereinafter may be collectively referred to as “MFD”) is progressing.

  As a technique related to an inspection method using MFD, for example, Japanese Unexamined Patent Application Publication No. 2015-177806 (Patent Document 1) includes an MFD having a flow channel into which a mixed liquid of a test bacteria solution and an antibacterial drug is introduced. It describes that an image obtained by culturing and observing the observation area of the flow path with a microscope or the like is obtained and analyzed to determine the antimicrobial susceptibility of bacteria or fungi derived from the test bacterial solution.

  In addition, as a technique related to inspection and diagnosis using a biochip, for example, in Japanese Translation of PCT International Publication No. 2010-525363 (Patent Document 2), measurement data for a result of analyzing a reaction performed on the biochip is recorded. A telemedicine diagnostic system is described that includes a bioanalyzer, a user terminal that transmits measurement data to a remote diagnostic server, and a remote diagnostic server. Here, on the user terminal, there is a virtual doctor consisting of software that outputs a diagnosis result by self-analysis based on the measurement data, and the remote diagnosis server connects the user to the virtual doctor or medical professional depending on the situation It is written to do so.

JP 2015-177806 A Special table 2010-525363 gazette

  For example, by using the technique described in Patent Document 1, the sensitivity of bacteria or fungi to antibacterial agents can be easily and quickly examined and confirmed. In addition, by applying a technique such as that described in Patent Document 2, it is possible to receive a judgment by a virtual doctor at the site of the examination or a judgment by a medical professional via a remote diagnosis server about the examination result. Yes, it is thought that it can support medical activities in the field.

  On the other hand, the site where the inspection of bacteria and the like that can be performed simply and quickly as described above is not limited to medical facilities such as hospitals and research facilities, but may be performed in various places. For example, it may be used in places that do not have sufficient environment and facilities, such as remote areas, isolated islands, battlefields, and epidemics. It can also be used in non-medical facilities such as airports. In these sites, there is a high possibility that a specialist or the like is absent. Therefore, it is necessary to perform the examination using the MFD more easily and quickly. On the other hand, it is necessary to make an appropriate determination on the inspection result, and to appropriately notify / instruct the determination content and the response content based on the determination content.

  Therefore, an object of the present invention is to support the execution of an inspection using MFD on the spot, and to support the MFD inspection that can appropriately notify / instruct the determination contents for the inspection result and the corresponding contents based on the determination result. An object is to provide an inspection method using a system and an MFD.

  The above and other objects and novel features of the present invention will be apparent from the description of this specification and the accompanying drawings.

  Of the inventions disclosed in this application, the outline of typical ones will be briefly described as follows.

  An MFD inspection support system according to a representative embodiment of the present invention includes an inspection apparatus that stores an MFD containing a specimen and a predetermined medicine and cultures the MFD under predetermined culture conditions, and the inspection apparatus and the network. A determination server that can be connected to the MFD, when the sample is unknown, a drug for sample identification is introduced as the drug, and when the sample is known, the MFD is sensitive as the drug. When a comparative drug is introduced and the drug is sealed in advance, culture condition information that can specify the corresponding culture condition is displayed.

  Further, the inspection device is stored in the culture condition selected from a plurality of preset culture conditions or the culture condition specified by the culture condition information displayed on the MFD. Incubating the MFD, photographing a predetermined observation area of the MFD being cultured, acquired image data, imaging situation data such as equipment and magnification when the image data was captured, the drug data, and the In the case where culture condition data and further attribute data relating to the specimen supply source are input, the attribute data (hereinafter, these may be collectively referred to as “culture information”) are determined as necessary. Send to server.

  Then, the determination server performs identification of the sample and / or determination of the effect on the drug as determination for the sample based on at least the image data among the data received from the inspection apparatus, and determines the determination result. Is transmitted to an information processing terminal possessed by the inspection device or an inspection executor who uses the inspection device, or can be viewed from the information processing terminal.

  Among the inventions disclosed in the present application, effects obtained by typical ones will be briefly described as follows.

  That is, according to the representative embodiment of the present invention, the execution of the inspection using the MFD in the field is supported, and the determination contents for the inspection result and the corresponding contents based on the inspection result are appropriately notified / instructed to the field. It becomes possible.

It is the figure which showed the outline | summary about the structural example of the MFD test | inspection assistance system which is one embodiment of this invention. It is the perspective view which showed the outline | summary about the example of the principal part of MFD in one embodiment of this invention. (A), (b) is the figure which showed the example of the image data which image | photographed the state near a flow path in the test | inspection by MFD in one embodiment of this invention. It is the processing flow figure which showed the outline | summary about the example of the flow of the process in one embodiment of this invention. It is the figure which showed the outline | summary about the example of the data structure of the test | inspection data storage DB of the determination server in one embodiment of this invention.

  Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. Note that components having the same function are denoted by the same reference symbols throughout the drawings for describing the embodiment, and the repetitive description thereof will be omitted. On the other hand, parts described with reference numerals in some drawings may be referred to with the same reference numerals although not illustrated again in the description of other drawings.

<System configuration>
FIG. 1 is a diagram showing an outline of a configuration example of an MFD inspection support system according to an embodiment of the present invention. In the MFD inspection support system 1 according to the present embodiment, the inspection executor 2 supports the inspection performed by the MFD 40 at the site, and transmits inspection result data to the determination server 10 via the network 20 such as the Internet. And it is a system which makes it possible for the inspection executor 2 to appropriately perform the subsequent response by notifying / instructing the inspection executor 2 of the determination result in the determination server 10 and the corresponding content.

  The site where the inspection is performed may be various places including places where sufficient environments and facilities are not provided, such as remote areas, isolated islands, battlefields, and epidemics. Further, the test performer 2 does not necessarily need to be a doctor or the like with specialized knowledge.

  In order to perform an inspection in such an environment, it is desirable that equipment required at the site is as simple as possible. In the present embodiment, the inspection apparatus 30 is used as an apparatus for culturing the MFD 40 containing a specimen and a predetermined drug on site for inspection. The inspection apparatus 30 has at least a function of housing the MFD 40 and culturing based on a predetermined culture condition, and a function of photographing a culture history and recording it as image data. The inspection apparatus 30 may be configured to execute some functions by the portable terminal 50 by cooperating with the portable terminal 50 such as a smartphone, a tablet-type terminal, or a notebook PC, and an application introduced therein.

  In the example of FIG. 1, the inspection device 30 is a dedicated portable device having a box shape, and has an insertion port 31 into which the MFD 40 is inserted. The inspection executor 2 can be accommodated in the inspection apparatus 30 by inserting the MFD 40 into the insertion port 31. In addition, such a storage means is an example to the last, For example, it can employ | adopt suitably about other structures, such as what uses an opening-and-closing lid | cover.

  As shown in Patent Document 1 and the like, by analyzing an image obtained by observing the flow path of the MFD 40, analysis such as susceptibility evaluation of a drug to bacteria can be performed. Also in the present embodiment, the inspection is performed by acquiring image data of a predetermined observation area of the flow path of the MFD 40. Therefore, it is desirable that the inspection apparatus 30 includes a camera 32 that can capture the image data by photographing the flow path of the stored MFD 40. When the inspection apparatus 30 includes the camera 32, it is desirable that the image data of the observation area can be captured at once. However, the inspection apparatus 30 includes a plurality of cameras that can individually and simultaneously capture each flow path in the observation area. There may be.

  Even when the inspection apparatus 30 does not include the camera 32, for example, the MFD 40 is stored in the inspection apparatus 30 or taken out from the inspection apparatus 30 using a mobile terminal 50 such as a smartphone having a camera function. It is good also as a structure which image | photographs. In this case, it is desirable that the inspection apparatus 30 includes a close-up lens that can be used by being connected to the lens portion of the mobile terminal 50. It is also desirable to attach to the image data culture information including the type of equipment such as the portable terminal 50 that has taken the image, and shooting status data such as magnification.

  In the example of FIG. 1, for convenience of explanation, it is described as if the camera 32 is installed outside the casing of the inspection apparatus 30, but in reality, the MFD 40 being cultured inside the inspection apparatus 30. It is installed at a position where the flow path can be photographed. Further, the camera 32 (or the portable terminal 50) may capture a flow path of the MFD 40 being cultured at a predetermined time interval, and acquire image data as a sequence of still images, or an image as a moving image. Data may be acquired. The inspection apparatus 30 further preferably includes an optical system unit that can provide a phase difference observation image or a dark field observation image in the photographing.

  The inspection apparatus 30 further has a communication function that can connect to the network 20 and transmit inspection data and image data to the determination server 10. The inspection device 30 itself may have a function of directly connecting to the network 20 by wireless / wired communication. Alternatively, it may not have a function of directly connecting to the network 20 but may be configured to connect to a communication module (not shown) having the function or the portable terminal 50 and perform communication via these.

  In addition, it may have a function of inputting / outputting data and displaying information via an external interface (not shown), an input / output device such as a keyboard, operation buttons, a display and a touch panel. For example, the examination performer 2 may be able to input and instruct various setting information such as attribute information of a specimen (subject) to be examined using the MFD 40 and culture conditions of the MFD 40.

  The MFD 40 preferably includes a memory chip 41 and / or a barcode 42 in which information related to the target MFD 40 is converted into data and recorded in advance in a machine-readable format such as electronic data or a code. . The memory chip 41 is configured by, for example, an IC chip having a nonvolatile semiconductor memory, a magnetic recording chip, or the like. As the barcode 42, a known one-dimensional or two-dimensional barcode can be appropriately used.

  The information related to the MFD 40 includes, for example, identification information such as a serial number and manufacturing date of the MFD 40. Further, when a predetermined drug is enclosed in the MFD 40 in advance, the drug data, default culture condition data, image capturing condition data, and the like may be included.

  The culture condition data is information for controlling conditions when the inspection apparatus 30 cultures the MFD 40, and includes, for example, temperature, humidity, culture time, and the like. A plurality of combinations and patterns of these culture conditions may be set in advance in the inspection apparatus 30, and any one of them may be designated. When the inspection device 30 can read these pieces of information from the memory chip 41 or the barcode 42, based on the read contents, a temperature or humidity adjusting mechanism, a thermometer, a hygrometer, a timer, or the like (not shown) is used. Set and control the culture environment.

  The shooting condition data is information for controlling conditions for shooting an image of the MFD 40. For example, the shooting method (still image, moving image), shooting timing, shooting interval, shooting time, projection light according to the culture state Types (ultraviolet rays, infrared rays, visible light, etc.). When the inspection device 30 can read these pieces of information from the memory chip 41 or the barcode 42, the camera 32 is set and controlled based on the read contents to perform photographing. The imaging condition data may include control information for selecting an individual flow path in the observation area and capturing an image.

  The test apparatus 30 uses, as the culture information, in addition to drug data and culture condition data, if available, attribute data of the specimen (subject), test date and time, a built-in GPS (Global Positioning System) sensor (not shown), and the like. The acquired information such as the examination location is transmitted to the determination server 10 via the network 20 in association with the captured image data.

  At this time, for example, the information inserted in the MFD 40 and the culture information such as the culture conditions suitable for the drug among these data are copied in advance along with the flow path in the predetermined observation area of the MFD 40. It can be displayed in a possible area by text or a barcode (two-dimensional code). In addition, the culture information acquired at the time of culture (inspection) can be converted into a barcode (two-dimensional code) or the like, synthesized with the image data of MFD 40, and superimposed. Thereby, even if the inspection apparatus 30 does not have a function of reading information from the memory chip 41 or the barcode 42, it can be transmitted to the determination server 10 together with the image data.

  Specifically, for example, these are read from a barcode 42 in which data such as drug data, culture conditions, and imaging conditions are recorded, and culture is performed in the inspection apparatus 30 based on the read data. Then, the inspection device 30 generates an image obtained by converting the data on the culture state and the photographing state into a two-dimensional code, and electronically superimposes the predetermined observation area on the photographed image to obtain image data. It transmits to the determination server 10.

  When the inspection device 30 does not have such an image composition function, for example, the image data is read into a portable terminal 50 such as a smartphone with a built-in dedicated image composition / transmission application prepared in advance, and composition processing is performed. The synthesized image data may be transmitted from the portable terminal 50 to the determination server 10. Furthermore, in the inspection apparatus 30 that does not have an image composition function, a preset symbol or the like may be displayed and described in a blank portion of a predetermined observation area with a stamp or the like, and this may be copied together with the flow path. As described above, the configuration in which the inspection apparatus 30 is further superimposed and combined with the captured image of the MFD 40 can be simplified and can be promoted to be widely installed in many sites.

  In the case where the inspection apparatus 30 can read information from the memory chip 41 or the barcode 42 and can accept input of information such as the attribute data of the specimen (subject), the inspection date and time, the inspection location, etc. If the information can be handled as data, the information may be transmitted to the determination server 10 in association with the captured image data. In association, for example, the serial number of the MFD 40 may be associated as text data using a key, or may be added as metadata to image data.

  The determination server 10 is a server system that accumulates test result data acquired from the inspection apparatus 30 installed at each site, and performs processing such as specimen identification, drug efficacy determination, and treatment / prescription determination. Information on the obtained determination results and on-site correspondence contents are recorded together with the inspection result data, and notified / instructed to the inspection executor 2 (specifically, for example, the inspection device 30 or the portable terminal 50). .

  The determination server 10 is a server system configured by, for example, a server device installed in a data center or the like, a virtual server constructed in a cloud computing environment, or the like. Each unit such as a data acquisition unit 11, a determination unit 12, and a result response unit 13 implemented as a software program that runs on middleware such as an OS (Operating System), a DBMS (DataBase Management System), and a Web server program (not shown). Have Each database includes an examination data storage database (DB) 14 and a prescription data DB 15.

  The data acquisition unit 11 has a function of receiving inspection data and image data transmitted from the inspection device 30 and the portable terminal 50 at each site via the network 20 and recording / accumulating them in the inspection data storage DB 14. The determination unit 12 has a function of determining the inspection content based on the data recorded in the inspection data storage DB 14 and outputting the result. Here, the determination unit 12 performs primary determination such as automatically identifying the virus type of the specimen or determining the medicinal efficacy of the drug by matching with data stored in the test data storage DB 14 in the past. If necessary, another examination under different culture conditions may be instructed. At this time, for example, based on the past accumulated data, weighting specific data such as the number of affected persons in the region, the age of the subject, setting the drug used for reexamination, that is, MFD40 and culture conditions, etc. The determination unit 12 is preferably provided with a learning function.

  Furthermore, based on the result of the above primary determination, the prescription data of the medicine registered in advance in the prescription data DB 15 is referred to as necessary, information related to the prescription is acquired, and this is instructed. The next determination may be performed. Not only automatic determination by the determination unit 12 but also information such as examination data, image data, determination results, etc. is presented to doctors with specialized knowledge, and inputs of determination results and instructions for corresponding contents are accepted. There may be.

  The result response unit 13 has a function of responding information such as the determination result in the determination unit 12 to the site. Data may be sent to the inspection device 30 or the portable terminal 50 by e-mail or other means. Moreover, it is good also as a structure which receives the access from the tester 2 via the portable terminal 50 etc., displays a determination result etc. on a screen, or transmits data.

  Further, it has an interface function (not shown) for performing access control such as determination of permission / refusal and reference range restriction in response to an access request from an external system or a user for data such as the inspection data storage DB 14. Good. For example, it is possible to make it possible to refer to and browse test result data stored in the test data storage DB 14 not only for a specific specimen (subject) but also for a doctor with specialized knowledge. . In addition, information may be disclosed to the government, the media, other medical institutions, etc., limited to data relating to specific virus types.

  According to the test result data stored in the test data storage DB 14, for example, for specific epidemic diseases, it is possible to quickly obtain endemic areas, types of epidemic diseases, and drug information with high medicinal properties for the diseases. Therefore, it is possible to predict the prevalence of the disease at an early stage, select preventive drugs and prescription drugs with high medicinal effects, and adjust their production.

<MFD>
FIG. 2 is a perspective view showing an outline of an example of a main part of the MFD 40. The MFD 40 shown in the example of FIG. 2 includes, for example, an introduction port 43 for introducing a test solution containing a specimen (also referred to as a bacterial suspension when the specimen is a bacterium), and four flow paths 44 branched therefrom. Each configuration of the pocket portion 45 and the discharge port 46 corresponding to each flow path 44 is provided. In the example of FIG. 2, for the sake of convenience, the concave groove and the like are all exposed, but in reality, all parts other than the introduction port 43 and the discharge port 46 are covered and sealed with resin or the like. Note that the configuration of the MFD 40 is not limited to the above, and MFDs having other configurations including those generally used can be used as appropriate. In the example of FIG. 2, an example in which one set of the above-described configuration is provided is shown. However, one MFD 40 may include a plurality of sets of the above-described configuration.

  Since the detailed principle of the inspection by the MFD 40 is the same as that of the known technique, the description is omitted. In the present embodiment, for example, in each of the pocket portions 45 and the corresponding flow paths 44, for comparison, those in which drugs are not introduced (standard flow paths) and drugs having different conditions are introduced. And have. The specimen of the test solution introduced from the introduction port 43 reacts with each drug or the like on each channel 44. By comparing the state of the sample in the standard channel at that time and the state of the sample in the other channel 44 that reacts with the drug based on image data obtained by photographing a predetermined observation area near the channel 44. The presence or absence of the drug effect is determined. In addition, when the specimen can be predicted to some extent and the reaction to the drug is clearly apparent, it is not always necessary to use the standard flow path. Similarly, when the specimen can be predicted to some extent, a known image stored in the determination server can be used for comparison instead of an image of the shape, form, and operation of the specimen in the standard channel. .

  Note that the subject to be tested is not limited to humans, and may be living things such as livestock and pets, plants such as trees and crops, soil and seawater. In addition, as described above, a medicine to be introduced into the pocket portion 45 of the MFD 40 may be pre-filled with a predetermined medicine, or a medicine according to the situation (for example, one instructed from the determination server 10). You may make it introduce | transduce from the discharge port 46 (drug | medicine introduction port) at the time of a test | inspection.

  FIG. 3 is a diagram illustrating an example of image data obtained by photographing the state near the flow path 44 in the inspection by the MFD 40. 3 (a) and 3 (b) both show the state of the four flow paths 44 in the MFD 40, and the left end flow path 44 is a standard flow path having no effect of the drug, and is from left to right. It shows that the effect of the drug is greater in the flow path 44. In addition, a bar code 47 such as a two-dimensional code in which the culture information is converted is synthesized.

  In the example of FIG. 3A, an example in which presence / absence of a drug effect is determined based on a shape difference between specimens is shown. Here, in contrast to the rounded-shaped bacteria in the standard flow path where there is no drug effect, in the flow path 44 where the other drug effects are effective, cell division is inhibited from the left side to the right side. It grows in the shape of a rod because the growth proceeds without being able to divide. → The morphological difference is observed that the cell membrane of the fungus cannot withstand the elongation and ruptures (dissolves) and disappears. By determining such a geometric difference or change by image processing in the determination unit 12 of the determination server 10, it is possible to perform the presence or absence of the effect of the drug, the identification of the bacterial species based on this, and the like.

  On the other hand, the example of FIG. 3B shows an example in which the presence / absence of the effect of the drug is determined based on the difference in the activity level of the specimen, that is, the degree of movement. Therefore, the image data used for the determination is acquired by continuous shooting of a moving image or a still image at a short interval so that the motion can be determined. In the example in the figure, one frame is shown for convenience. In contrast to the bacteria that are actively moving in the standard flow path where there is no drug effect, the particle shape does not change in the other flow path 44 where the drug effect is effective, but gradually increases as the drug effect increases. There is a difference that the bacteria stop working. Similar to the case of FIG. 3A, such a difference or change in the activity level is determined by image processing in the determination unit 12 of the determination server 10, so that the presence or absence of the effect of the drug and the bacterial species based on this Can be identified.

<Process flow>
FIG. 4 is a process flow diagram showing an overview of an example of a process flow in the MFD inspection support system 1 of the present embodiment. First, at various sites, the test executor 2 acquires a biological tissue or the like as a specimen from a specimen provider (subject or the like) (S01). Then, the test performer 2 selects the MFD 40 to be used for the test according to the contents of the acquired sample (S02). For example, when the specimen is unknown, the MFD 40 in which the specimen identifying drug is enclosed is selected (or the specimen identifying drug is introduced into the MFD 40). On the other hand, when the specimen is known, the MFD 40 in which the drug for sensitivity comparison is enclosed is selected (or the drug for sensitivity comparison is introduced into the MFD 40).

  As the drug for specimen identification, for example, a drug for Gram-negative bacteria or Gram-positive bacteria can be used. In this case, the bacterial species as the specimen is roughly classified based on the result of the first examination (primary examination), and further, a second examination (secondary examination) by the MFD 40 in which the medicine for each classified bacterial species is encapsulated. By performing (inspection), it is possible to identify a detailed bacterial species. Further, a reagent for antigen-antibody reaction may be used as a drug for specimen identification. In this case, the virus type can be identified based on the result of the primary inspection.

  On the other hand, as a drug for sensitivity comparison, for example, a drug for Pseudomonas aeruginosa can be used. In this case, even if it is known to be Pseudomonas aeruginosa, there are various subspecies of Pseudomonas aeruginosa. Therefore, in order to examine the sensitivity of which drug has an effect, a plurality of kinds of Pseudomonas aeruginosa drugs and Pseudomonas aeruginosa drugs with different concentrations are used. Thereby, it is possible to know the type and amount of the drug that is highly sensitive to the specimen, that is, has a high effect.

  When the MFD 40 to be used is selected, a sample (and a drug as necessary) is introduced into the MFD 40, the MFD 40 is prepared, inserted into the inspection apparatus 30, and cultured under predetermined culture conditions (S03). In order to simplify the on-site procedure and the configuration of the inspection apparatus 30, it is desirable to select and use a predetermined culture condition from a plurality of preset culture conditions. Although the test performer 2 may input / select the culture conditions, even in this case, information for specifying the culture conditions is displayed / described on the MFD 40 to be used so that the test performer 2 can easily grasp the culture conditions. Is desirable. Furthermore, information for specifying the culture conditions is recorded and displayed on the memory chip 41 and the barcode 42 of the MFD 40, and the inspection apparatus 30 reads this and performs culture by setting the culture conditions according to the contents of the information. If so, it is more desirable.

  During the culture, the camera 32 of the inspection apparatus 30 automatically acquires image data such as still images and moving images near the flow path 44 of the MFD 40 and still images taken continuously. Alternatively, the examination executor 2 performs imaging using the mobile terminal 50. In addition, when the attribute data and the like related to the sample provider can be obtained and can be input to the inspection apparatus 30, the test performer 2 inputs these data. For example, it is possible to input information such as the age, sex, and examination location of the subject.

  When the culture in the inspection apparatus 30 is completed, as the test result, the drug data, the culture condition data, and the attribute data (that is, the culture information) related to the sample provider if acquired are associated with the image data of the MFD 40 Is transmitted to the determination server 10 (S04). As described above, the culture information may be converted into a barcode (two-dimensional code) and electronically synthesized with the image data of the MFD 40. In the determination server 10 that has received the data, the data acquisition unit 11 records and accumulates them in the inspection data accumulation DB 14 (S11). Then, the determination unit 12 performs primary determination on the inspection result (S12_1).

  In the primary determination, for example, as shown in FIG. 3 described above, the presence or absence and the degree of the effect of the drug are determined based on the image data, and the bacterial species and virus species are identified. And / or the drug effective for the specimen, that is, the drug to be prescribed, the amount thereof, and the like are specified. The determination result is reflected in the inspection data storage DB 14.

  After that, if necessary, secondary determination for determining the contents of treatment, prescription, reexamination, etc. at the site may be performed (S12_2). For example, when the bacterial species or virus species cannot be identified in the above primary determination, the bacterial species or virus identified in the past determination results accumulated in the examination data storage DB 14 by the determination unit 12 is further used. Matching may be performed based on the correspondence relationship between the species and the type or effect of the drug, and identification or the like may be determined. Furthermore, it is possible to further improve the matching accuracy by using attribute data information relating to the sample provider (subject, etc.), information on the examination site, and the like as targets of matching.

  If the identification is completed, information on the prescription may be acquired and instructed by referring to prescription data of a drug registered in the prescription data DB 15 in advance. If identification is not possible even in the secondary determination, a second inspection (secondary inspection) may be instructed as a determination result. At this time, for example, it may be instructed to use the medicine or the culture condition of the one determined to be the closest in the matching in the secondary examination.

  In addition, the secondary determination is not limited to the automatic determination as described above, but presents the contents of the examination data storage DB 14 to a doctor or the like having specialized knowledge in the relevant field, so that treatment and prescription can be performed. You may make it receive the input of instruction | indications, such as the content and the necessity of a secondary inspection. A doctor or the like accesses the determination server 10 via a network such as the network 20 or a LAN (Local Area Network) using an information processing terminal such as a PC (not shown), and browses or instructs the contents of the examination data storage DB 14. The contents can be input.

  Thereafter, the result response unit 13 notifies the examination performer 2 of the determination result (S13). The determination result may be notified to the inspection device 30 and displayed on a display (not shown) or may be notified to the portable terminal 50 of the inspection executor 2 by e-mail or other means.

  In the field, when the determination result is acquired (S05), the content thereof, that is, the information of the identified bacterial species and virus species, the information on the effect of the drug, the treatment content instructed by the determination server 10 or the specialist, Based on information such as prescription, the test executor 2 performs appropriate medical treatment such as appropriate treatment or drug prescription (S06). When a second examination (secondary examination) is instructed by the determination server 10 or a specialist, a secondary examination is performed based on the other specified medicine and culture conditions. In this case, the same inspection is repeated again by returning to the above-described step S01 or step S02.

<Data structure>
FIG. 5 is a diagram showing an outline of an example of the data configuration of the inspection data storage DB 14 of the determination server 10. The inspection data storage DB 14 is a table that holds the contents of inspection performed by the inspection device 30 at each site, the result data thereof, and information on the determination results for the contents. For example, MFD serial number, date of manufacture, drug data, culture condition data, image data, determination result, data disclosure availability, test date / time, test site information, test performer information, test target type, test subject age, test subject sex, etc. Has each item.

  The MFD serial number item holds serial number information that uniquely identifies the MFD 40 used for the target inspection. Moreover, the item of a manufacture date hold | maintains the information of the date on which the said MFD40 was manufactured. Such information is recorded in, for example, the memory chip 41 or the barcode 42 of the MFD 40.

The item of drug data holds information such as the type and concentration of the drug used for the target examination.
As shown in the example of FIG. 2, when there are a plurality of flow paths 44 in the MFD 40 and a plurality of drugs can be used at the same time, information on each drug is held. The item of the culture condition data holds information relating to the contents of the culture conditions set in the inspection apparatus 30 in the target inspection. The item of image data holds information related to image data in the vicinity of the flow path 44 of the MFD 40 taken by the inspection device 30 or the portable terminal 50 in the target inspection. The image data itself may be held, or the image data may be recorded on the hard disk as a file, and the file name and path information may be held.

  The item of the determination result holds information on the result determined by the determination server 10 or the specialist with respect to the result of the target examination. For example, information regarding the results of identification of bacterial species and virus species, drug efficacy, treatment and prescription content, secondary examination instructions, and the like may be included. The data disclosure availability item holds information such as a code value and a flag for specifying whether or not the data relating to the subject inspection can be disclosed to the outside. When the information can be disclosed, information related to access control such as which items may be disclosed up to which range may be held.

  Each item of the inspection date / time, the inspection site information, and the inspection person information specifies the date / time when the target inspection was performed, information such as a code value for specifying the inspection area or the inspection area, and the inspection person 2. Holds information such as name and ID. In addition, each item of the test target type, the age of the subject, and the sex of the test subject is the type of the sample providing source (human, livestock, crop, etc.) in the target test, and the test target type is human. Holds subject's age and gender information.

  Note that the data structure (items) of the table shown in FIG. 5 is merely an example, and any other table structure or data structure may be used as long as similar data can be held and managed. Good.

  As described above, according to the MFD examination support system 1 which is an embodiment of the present invention, the examination executor 2 inserts the MFD 40 into which the specimen and the predetermined medicine are introduced into the examination apparatus 30 at the site. Thus, an example operation of culturing and transmitting the test result to the determination server 10 can be performed simply and quickly. In addition, since the inspection device 30 can be configured simply, it is possible to perform the inspection without selecting a site. In addition, by notifying and instructing the inspection executor 2 of the determination result and the corresponding content in the determination server 10, it is possible for the inspection executor 2 having no specialized knowledge to appropriately perform the subsequent response. It becomes.

  As mentioned above, the invention made by the present inventor has been specifically described based on the embodiments. However, the present invention is not limited to the above-described embodiments, and various modifications can be made without departing from the scope of the invention. Needless to say. For example, the above-described embodiment has been described in detail for easy understanding of the present invention, and is not necessarily limited to the one having all the configurations described. In addition, it is possible to add, delete, and replace other configurations for a part of the configuration of the above-described embodiment.

  For example, in the present embodiment, when the specimen is unknown, after identifying the bacterial species using the MFD in which the chemical for specimen identification is encapsulated, it is again used for sensitivity comparison corresponding to the identified bacterial species. In the MFD in which the drug is encapsulated, a highly effective drug is selected for the identified bacterial species. However, increasing the number of MFD channels (using MFDs with a large number of channels) ), From the identification of the bacterial species to the selection of a drug with high medicinal effect (sensitivity test), it may be performed simultaneously or continuously in the inspection apparatus.

  Further, the inspection apparatus may have a mechanism capable of receiving the instruction signal from the determination server and controlling the imaging conditions. In that case, the inspection apparatus acquires image data as a moving image or a plurality of continuous still images and transmits the acquired image data to the determination server in the initial stage of the inspection. Based on the image data acquired from the inspection device, this determination server or a person with specialized knowledge in the field (hereinafter, also referred to as a determination server or the like) Select one bacterium). The determination server or the like transmits imaging conditions that can clearly determine the shape, state, and operation of the specific individual to the inspection apparatus. The inspection device that has received the imaging condition can acquire a suitable image for the specific individual by controlling the imaging condition based on the received imaging condition. In addition, when the inspection apparatus has a function capable of autonomously controlling preferable imaging conditions, the inspection apparatus autonomously controls the imaging conditions for the specific individual selected by the determination server or the like, and A suitable image can be acquired for an individual. As a result, clear photographed images can be acquired sequentially even for fast-moving bacterial species, small-diameter bacterial species, etc., regardless of the superiority or inferiority of the skill of the photographer, and highly accurate determination is possible. .

  As described above, it may have a mechanism for controlling the imaging conditions of the inspection apparatus from the determination server side in real time to track and image a specific individual. Even when there is a change in movement or growth in the bacteria of a specific individual in the specimen, the change is tracked with an image and can be determined in a short time. The setting of the shooting conditions when shooting by tracking the specific individual is controlled so as to be associated with the target bacterial species.

  In addition, for a camera built in the inspection apparatus, a general bacterial disease can be obtained by setting the depth of field to ½ or less (for example, 20 μm or less) of the flow path height of the MFD to be used. In the identification and susceptibility evaluation of the bacteria causing the cause, it is possible to clearly distinguish the non-photographing bacteria that overlap behind (flow path height direction) from the photographing target bacteria, thus simplifying the camera focus adjustment. be able to. Here, the channel height refers to the inner dimension of the channel along the observation (photographing) direction. The setting of the depth of field is controlled so as to be associated according to the target bacterial species and the like.

  For example, in the present embodiment, the determination result for the inspection performed at the site using the MFD 40 is notified and fed back to the inspection performer 2 at the site, but the configuration is limited to the configuration that feeds back to the site. I can't. For example, when traveling abroad, the specimen acquired before the movement from the moving person is cultured in the inspection apparatus 30 installed at the base of the movement source (airport, port, etc.), and the determination is made by transmitting the inspection result to the determination server 10. Do. Then, based on the determination result, whether to permit or reject the movement may be determined, and the information may be notified to the destination site in advance. Thereby, the movement of the mover who has been notified of the movement non-permission can be regulated at the beach at the destination site.

  In the present embodiment, the description has been mainly made on the test for bacteria, but it goes without saying that the present invention can also be applied to viruses. In addition, the above-described MFD 40 is generally used for culture (inspection) using the channel 44 having a channel height of micrometer (μm) level, and the channel height is several tens to several hundreds of nanometers. It can also be diverted to the MFD 40 having the level channel 44.

  INDUSTRIAL APPLICABILITY The present invention can be used for an MFD inspection support system that automatically performs determination based on data of an inspection performed on site using an MFD and an inspection method that uses an MFD.

DESCRIPTION OF SYMBOLS 1 ... MFD inspection support system, 2 ... Examiner, 10 ... Determination server, 11 ... Data acquisition part, 12 ... Determination part, 13 ... Result response part, 14 ... Examination data storage DB, 15 ... Prescription data DB, 20 ... Network, 30 ... Inspection device, 31 ... Insertion port, 32 ... Camera, 40 ... MFD, 41 ... Memory chip, 42 ... Bar code, 43 ... Introduction port, 44 ... Flow path, 45 ... Pocket part, 46 ... Discharge port, 47 ... Barcode,
50 ... Mobile terminal

Claims (16)

An MFD inspection support system that supports inspection by a microchannel device (MFD),
A test apparatus that stores the MFD containing the sample and a predetermined medicine and cultures the MFD under a predetermined culture condition; and a determination server that can be connected to the test apparatus via a network,
In the MFD, when the sample is unknown, a drug for sample identification is introduced as the drug. When the sample is known, a drug for sensitivity comparison is introduced as the drug. When the drug is pre-encapsulated, culture condition information that can specify the corresponding culture condition is displayed,
The inspection apparatus stores the MFD stored under the culture condition selected from a plurality of preset culture conditions or the culture condition specified by the culture condition information displayed on the MFD. If the acquired image data, the drug data, the culture condition data, and the attribute data relating to the specimen supply source are input, the MFD in culture is photographed. Sending attribute data to the determination server;
The determination server performs identification of the sample and / or determination of an effect relating to the medicine as determination for the sample based on at least the image data among each data received from the inspection apparatus, and determines the determination result as the determination result. An MFD inspection support system which transmits to an information processing terminal possessed by an inspection device or an inspection executor who uses the inspection device, or enables browsing from the information processing terminal. The MFD inspection support system according to claim 1,
In place of or in addition to the display of the culture condition information, the MFD displays the culture condition information in a machine-readable code and / or recorded on a memory chip included in the MFD. ,
The MFD inspection support system, wherein the inspection apparatus sets the culture condition based on the culture condition information read from the MFD. The MFD inspection support system according to claim 1,
In the MFD, information relating to imaging conditions when the inspection apparatus images the MFD being cultured is displayed in a machine-readable code and / or recorded on a memory chip provided in the MFD,
The inspection apparatus is an MFD inspection support system that images the MFD based on information related to the imaging condition read from the MFD. The MFD inspection support system according to claim 2,
The MFD examination support system, wherein the culture condition includes at least information related to setting of temperature, humidity, and culture time. In the MFD inspection support system according to claim 3,
The MFD inspection support system, wherein the photographing condition includes at least information on a classification of a moving image or a still image, a photographing interval when using a still image, and a type of projection light. The MFD inspection support system according to claim 1,
In the MFD, information including the drug data in addition to the culture condition information is displayed, and when the MFD photographs the MFD being cultured, the culture condition information displayed on the MFD and An MFD examination support system that obtains the image data by copying information including the drug data together. The MFD inspection support system according to claim 1,
The determination server is an MFD inspection support system that records each data received from the inspection apparatus and data related to the determination result in a database. The MFD inspection support system according to claim 7,
In addition to the determination for the specimen, the determination server further determines whether or not the inspection apparatus needs to be tested again based on the data accumulated in the database, or treatment and / or medicine for the specimen provider MFD inspection support system that determines prescription information. The MFD inspection support system according to claim 8,
The determination server is an MFD inspection support system that selects and outputs the medicine and the culture condition used in the second inspection when the second inspection is necessary in the inspection apparatus. The MFD inspection support system according to claim 7,
In addition to the determination on the sample, the determination server further accepts the input of the doctor's necessity for a second test in the test apparatus or information on treatment and / or drug prescription for the sample provider. , MFD inspection support system. The MFD inspection support system according to claim 10,
The determination server is an MFD inspection support system that receives and outputs an input of the medicine and the culture condition used by a doctor when the inspection apparatus needs to be inspected again. The MFD inspection support system according to claim 1,
The determination server analyzes shape characteristics of the state of the specimen in a flow channel into which a drug is introduced among a plurality of flow channels in the MFD based on the image data acquired from the inspection apparatus, and based on the analysis result An MFD examination support system that determines whether or not the drug has an effect on the specimen. The MFD inspection support system according to claim 1,
The inspection apparatus acquires the image data as a moving image or a plurality of continuous still images,
The determination server analyzes the state and the activity level of the sample in a flow channel into which a drug is injected among a plurality of flow channels in the MFD based on the image data acquired from the inspection apparatus, and based on the analysis result An MFD examination support system that determines whether or not the drug has an effect on the specimen. The MFD inspection support system according to claim 1,
The inspection apparatus acquires the image data as a moving image or a plurality of continuous still images,
The determination server selects a specific individual of the specimen in each flow channel in the MFD based on the image data acquired from the inspection apparatus, and can clearly determine the shape, state, and operation of the specific individual An MFD inspection support system that transmits imaging conditions to the inspection apparatus, and the inspection apparatus acquires the image data based on the received imaging conditions. The MFD inspection support system according to claim 1,
The determination server controls the depth of field to be one half or less of the flow path height of the flow path of the MFD as one of imaging conditions when the inspection apparatus captures the MFD. , MFD inspection support system. An inspection method using a microchannel device (MFD),
When the sample is unknown, the MFD into which the drug for sample identification is introduced is selected. When the sample is known, the MFD into which the drug for sensitivity comparison is introduced is selected, and the sample is selected in the selected MFD. A first step of inserting and inserting into the inspection device;
As the culture condition selected from a plurality of culture conditions set in advance by the inspection device, or when the drug is pre-encapsulated in the MFD, the corresponding culture condition is specified. In the culture conditions specified by the displayed culture condition information,
A second step of culturing the inserted MFD;
When the MFD is being imaged by the inspection device, and the acquired image data, the drug data, the culture condition data, and the attribute data relating to the sample provider are input A third step of transmitting the attribute data to the determination server via a network;
A fourth step in which the determination server performs identification of the sample and / or determination of an effect on the medicine as determination on the sample based on at least the image data among the data received from the examination apparatus; ,
The determination server transmits the determination result in the fourth step to the information processing terminal possessed by the inspection apparatus or an inspection executor who uses the inspection apparatus, or allows the information processing terminal to browse the information processing terminal. The inspection method using MFD which has these processes.